Rroxscaffold_1G00042190

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002199
Physical Location & Seq
Reverse (-)
60278945 .. 60283932
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00042190.1

Sequence Viewer

Length: 699 bp
ATGGTACTCATCGCTGAGCTGATGTGTGGGTTGAAGAAGGGGAGCGTTGTGGAGCTAACCCTGGTCACGGGGATAGGCTTCGAGATTGATCAGATCGAACTGCCAAAGAATTATCCTATGTTTCTTCTTTTCACAACGGGATCTGGAATCAGGTCTCTGATTGCGTCAGGGTTTAATGCTGGTAAGGGATCCGACGTGAAACTCTTCTATGGGGCCAGAAATCTTGACAGAATGGCTTATCAGAGCGAATCAAGAAGAGTAGTAAATGGTGGAGAGATGGGTTCAGCCTTGACAACAGCGGGATCAATGTATATGATTGAAGGTGAGAAGGGAATTTCGTATATGCATTTGTTTGTTGAGCATGAAAATGTTACAAACAGTAATTGGAGTTGCCTTACAGAGGGCATTACCATTATTTTCATACATCCTGGAGCTTTCAATATTCATCACAAATTAAATGTTCTTGGAGTGGATTTGGAAAAGGTGGCAGAAATAGCAACAACATCATCTGCTTTGAAAGAGGAAGAGATCAAGCTGCGTGTCAAAATAGCAATTCACCAAGATGAAAATCTGGTTCAGAAAATGGCAGACTGGGCCAATTCTATTGTGGAGGAGCTAATGAATGATGAGCAGCCAAATACCATGGAAGTTGGTGACTGGAATGCTGCTTATTCAGATTTTGAGCACTTAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

232

Amino Acids

25.68

Weight (kDa)

4.93

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 299
AclWI GGATC 4 cut(s) 148, 183, 196, 310
AcsI RAATTY 1 cut(s) 333
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 2 cut(s) 67, 400
AgsI TTSAA 4 cut(s) 34, 320, 439, 517
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 2 cut(s) 60, 427
AluBI AGCT 5 cut(s) 19, 55, 434, 535, 616
AluI AGCT 5 cut(s) 19, 55, 434, 535, 616
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 4 cut(s) 148, 183, 196, 310
AoxI GGCC 2 cut(s) 213, 594
ApeKI GCWGC 3 cut(s) 535, 631, 665
ApoI RAATTY 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 213, 594
AsuHPI GGTGA 3 cut(s) 335, 548, 665
BamHI GGATCC 1 cut(s) 188
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 3 cut(s) 522, 643, 652
BccI CCATC 1 cut(s) 271
BciT130I CCWGG 2 cut(s) 62, 429
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 159
BisI GCNGC 3 cut(s) 536, 632, 666
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 3 cut(s) 537, 633, 667
Bme1390I CCNGG 2 cut(s) 62, 429
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 213, 594
BmiI GGNNCC 2 cut(s) 190, 214
BmrFI CCNGG 2 cut(s) 62, 429
BmrI ACTGGG 1 cut(s) 601
BmuI ACTGGG 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 450
Bpu1102I GCTNAGC 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 60, 642
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 400
Bse1I ACTGG 2 cut(s) 596, 662
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 62, 429
BseDI CCNNGG 2 cut(s) 60, 642
BseGI GGATG 1 cut(s) 424
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 67, 400
BseMII CTCAG 1 cut(s) 6
BseNI ACTGG 2 cut(s) 596, 662
BseRI GAGGAG 1 cut(s) 626
BseXI GCAGC 3 cut(s) 522, 643, 652
BshFI GGCC 2 cut(s) 215, 596
BsiHKAI GWGCWC 1 cut(s) 687
BslI CCNNNNNNNGG 2 cut(s) 67, 400
BsmAI GTCTC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 2 cut(s) 215, 596
Bso31I GGTCTC 1 cut(s) 159
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 6 cut(s) 88, 93, 140, 188, 302, 528
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 1 cut(s) 299
BspANI GGCC 2 cut(s) 215, 596
BspCNI CTCAG 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 190, 214
BspPI GGATC 4 cut(s) 148, 183, 196, 310
BspTNI GGTCTC 1 cut(s) 159
BsrI ACTGG 2 cut(s) 596, 662
BssECI CCNNGG 2 cut(s) 60, 642
BssMI GATC 6 cut(s) 88, 93, 140, 188, 302, 528
BssT1I CCWWGG 1 cut(s) 642
Bst2UI CCWGG 2 cut(s) 62, 429
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 3 cut(s) 209, 250, 519
BstDEI CTNAG 2 cut(s) 15, 688
BstDSI CCRYGG 1 cut(s) 642
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 424
BstKTI GATC 6 cut(s) 91, 96, 143, 191, 305, 531
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 6 cut(s) 88, 93, 140, 188, 302, 528
BstMWI GCNNNNNNNGC 2 cut(s) 494, 593
BstNI CCWGG 2 cut(s) 62, 429
BstSCI CCNGG 2 cut(s) 60, 427
BstV1I GCAGC 3 cut(s) 522, 643, 652
BstX2I RGATCY 2 cut(s) 140, 188
BstYI RGATCY 2 cut(s) 140, 188
BsuRI GGCC 2 cut(s) 215, 596
BtgI CCRYGG 1 cut(s) 642
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 213, 594
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 5
CviAII CATG 2 cut(s) 362, 643
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 2 cut(s) 15, 688
DpnI GATC 6 cut(s) 90, 95, 142, 190, 304, 530
DpnII GATC 6 cut(s) 88, 93, 140, 188, 302, 528
Eam1104I CTCTTC 3 cut(s) 209, 250, 519
EarI CTCTTC 3 cut(s) 209, 250, 519
Eco130I CCWWGG 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRII CCWGG 2 cut(s) 60, 427
EcoT14I CCWWGG 1 cut(s) 642
EcoT22I ATGCAT 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 2 cut(s) 365, 646
FaiI YATR 9 cut(s) 119, 210, 312, 314, 342, 344, 363, 422, 644
FatI CATG 2 cut(s) 361, 642
FauI CCCGC 1 cut(s) 292
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 3 cut(s) 536, 632, 666
FokI GGATG 1 cut(s) 411
Fsp4HI GCNGC 3 cut(s) 536, 632, 666
GluI GCNGC 3 cut(s) 536, 632, 666
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 215, 596
HgaI GACGC 1 cut(s) 153
Hin1II CATG 2 cut(s) 365, 646
HinfI GANTC 2 cut(s) 147, 248
HphI GGTGA 3 cut(s) 335, 548, 665
Hpy188I TCNGA 6 cut(s) 93, 159, 193, 243, 579, 676
Hpy188III TCNNGA 4 cut(s) 82, 144, 224, 252
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 3 cut(s) 31, 314, 322
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 593
HpyF3I CTNAG 2 cut(s) 15, 688
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 2 cut(s) 365, 646
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 6 cut(s) 88, 93, 140, 188, 302, 528
LmnI GCTCC 4 cut(s) 42, 52, 431, 613
Lsp1109I GCAGC 3 cut(s) 522, 643, 652
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 64, 370, 653
MalI GATC 6 cut(s) 90, 95, 142, 190, 304, 530
MboI GATC 6 cut(s) 88, 93, 140, 188, 302, 528
MboII GAAGA 5 cut(s) 46, 116, 196, 267, 536
MflI RGATCY 2 cut(s) 140, 188
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 6 cut(s) 109, 333, 382, 452, 552, 598
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 3 cut(s) 394, 514, 604
Mph1103I ATGCAT 1 cut(s) 348
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 2 cut(s) 174, 455
MslI CAYNNNNRTG 2 cut(s) 366, 561
MspA1I CMGCKG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 62, 429
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 2 cut(s) 62, 429
MwoI GCNNNNNNNGC 2 cut(s) 494, 593
NcoI CCATGG 1 cut(s) 642
NdeII GATC 6 cut(s) 88, 93, 140, 188, 302, 528
NlaIII CATG 2 cut(s) 365, 646
NlaIV GGNNCC 2 cut(s) 190, 214
NmuCI GTSAC 2 cut(s) 64, 653
NsiI ATGCAT 1 cut(s) 348
PctI GAATGC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 147, 248
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 3 cut(s) 537, 633, 667
Psp6I CCWGG 2 cut(s) 60, 427
PspGI CCWGG 2 cut(s) 60, 427
PspN4I GGNNCC 2 cut(s) 190, 214
PspPI GGNCC 2 cut(s) 213, 594
PsuI RGATCY 2 cut(s) 140, 188
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
RseI CAYNNNNRTG 2 cut(s) 366, 561
SaqAI TTAA 2 cut(s) 174, 455
SatI GCNGC 3 cut(s) 536, 632, 666
Sau3AI GATC 6 cut(s) 88, 93, 140, 188, 302, 528
Sau96I GGNCC 2 cut(s) 213, 594
ScrFI CCNGG 2 cut(s) 62, 429
SduI GDGCHC 1 cut(s) 687
SetI ASST 9 cut(s) 21, 57, 155, 198, 325, 436, 486, 537, 618
SmiMI CAYNNNNRTG 2 cut(s) 366, 561
Sse9I AATT 6 cut(s) 109, 333, 382, 452, 552, 598
SsiI CCGC 1 cut(s) 299
SspI AATATT 1 cut(s) 442
StyD4I CCNGG 2 cut(s) 60, 427
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 1 cut(s) 198
TaqI TCGA 2 cut(s) 81, 96
TasI AATT 6 cut(s) 109, 333, 382, 452, 552, 598
TfiI GAWTC 2 cut(s) 147, 248
Tru1I TTAA 2 cut(s) 174, 455
Tru9I TTAA 2 cut(s) 174, 455
TseFI GTSAC 2 cut(s) 64, 653
TseI GCWGC 3 cut(s) 535, 631, 665
Tsp45I GTSAC 2 cut(s) 64, 653
TspDTI ATGAA 5 cut(s) 378, 409, 434, 579, 635
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 1 cut(s) 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.