RLG00000006720

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002199
Physical Location & Seq
Forward (+)
9922890 .. 9925259
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006720

Sequence Viewer

Length: 816 bp
ATGTCTATCCTACGCCGCCTCAACCCCTCGCACCTAACCCTCCACCGCCACCGCCGCCGCCTCGCAACCGTCGCAGCCGCCGTACGCCAAGACACCAGTCTCTGGACCCCGGCCCCGCTCTCCCGGGTTGAGCCTGCCGCCGAGTCCCTCTTCCATGTCCGCGTCGACCTCTCCGACGCGCCCGACCTCGCGTCCTCCTACACGCGCGCCGGGCAGTACCTCCAGCTCCGCGTCCCGGACGTTGAAAAGCCCTCGTTCTTGGCCATTGCGTCGCCGCCGTCCCTGGCGGCGAAGGGCGTGTTCGAGTTCTTGGTGAAGAGCGTGGAGGGGTCCATCGCTGAGCTGCTGTGCGGGTTGAAGAAGGGGGATGTTGTGGAGCTGACCCAGGCCATGGGGAAAGGCTTCGAGATTGATCAGATCGAGCCGCCGGAGAATTATCCTACGGTTCTAATTTTCGCCACGGGGTCTGGAATCAGTCCAATCAGGTCTCTGATTGAATCAGGGTTTGACGCCGACAAGCGATCCGACGTGAAGCTCTTCTATGGGGCCAGAAACCTTGACAGAATGGCTTATCAGGATAGATTTAAAGACTGGGAAGCTTCAGGTGTAAAGATAGTTCCGGTATTGTCACAACCACACGACGGCTGGACTGGACAAAGTGGTTATGTGCAGGCTGCTTTTACTAGAGCCAAGCAAGTTTATAACCCTCTCTCCACCGCTGCTGTTCTCTGTGGGCAGAAACAGATGGCTGAGGAAGTTACCTCAGTTCTTTTAGCAGATGGAGTTGCAAGTGAAAAAATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

272

Amino Acids

29.67

Weight (kDa)

7.77

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 153 - 253 1.3e-16 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AccB7I CCANNNNNTGG 2 cut(s) 102, 391
AccBSI CCGCTC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 165
AccII CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
AclWI GGATC 1 cut(s) 516
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 510
AfaI GTAC 2 cut(s) 84, 218
AfiI CCNNNNNNNGG 4 cut(s) 102, 235, 391, 641
AgsI TTSAA 3 cut(s) 245, 358, 497
AhdI GACNNNNNGTC 1 cut(s) 190
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 2 cut(s) 282, 384
AluBI AGCT 5 cut(s) 226, 343, 379, 535, 599
AluI AGCT 5 cut(s) 226, 343, 379, 535, 599
Alw26I GTCTC 2 cut(s) 104, 492
AlwI GGATC 1 cut(s) 516
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 123
AoxI GGCC 4 cut(s) 111, 261, 387, 546
ApeKI GCWGC 4 cut(s) 74, 343, 674, 719
ApoI RAATTY 1 cut(s) 808
Asp700I GAANNNNTTC 3 cut(s) 401, 536, 809
AspLEI GCGC 3 cut(s) 181, 207, 209
AspS9I GGNCC 4 cut(s) 105, 112, 330, 546
AsuC2I CCSGG 5 cut(s) 110, 124, 125, 211, 236
AsuHPI GGTGA 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 123
AvaII GGWCC 2 cut(s) 105, 330
BalI TGGCCA 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 750
BbvI GCAGC 4 cut(s) 86, 330, 661, 706
BccI CCATC 3 cut(s) 341, 739, 773
BceAI ACGGC 3 cut(s) 65, 262, 658
BciT130I CCWGG 2 cut(s) 284, 386
BclI TGATCA 1 cut(s) 412
BcnI CCSGG 5 cut(s) 110, 124, 125, 211, 236
BcoDI GTCTC 2 cut(s) 104, 492
BfaI CTAG 1 cut(s) 684
BlpI GCTNAGC 1 cut(s) 339
Bme1390I CCNGG 7 cut(s) 110, 124, 125, 211, 236, 284, 386
Bme18I GGWCC 2 cut(s) 105, 330
BmeRI GACNNNNNGTC 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 123
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 4 cut(s) 105, 112, 330, 546
BmiI GGNNCC 4 cut(s) 107, 114, 331, 547
BmrFI CCNGG 7 cut(s) 110, 124, 125, 211, 236, 284, 386
BmrI ACTGGG 1 cut(s) 601
BmuI ACTGGG 1 cut(s) 601
BoxI GACNNNNGTC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 339
BpuMI CCSGG 5 cut(s) 110, 124, 125, 211, 236
BsaHI GRCGYC 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 6 cut(s) 108, 123, 282, 384, 390, 459
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 2 cut(s) 695, 725
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 235, 391, 641
Bse1I ACTGG 3 cut(s) 96, 596, 655
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 2 cut(s) 284, 386
BseDI CCNNGG 6 cut(s) 108, 123, 282, 384, 390, 459
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 4 cut(s) 102, 235, 391, 641
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 3 cut(s) 330, 741, 777
BseNI ACTGG 3 cut(s) 96, 596, 655
BsePI GCGCGC 1 cut(s) 205
BseXI GCAGC 4 cut(s) 86, 330, 661, 706
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
BshFI GGCC 4 cut(s) 113, 263, 389, 548
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 6 cut(s) 110, 124, 210, 236, 428, 620
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 3 cut(s) 130, 218, 265
BslI CCNNNNNNNGG 4 cut(s) 102, 235, 391, 641
BsmAI GTCTC 2 cut(s) 104, 492
BsmFI GGGAC 3 cut(s) 130, 218, 265
BsnI GGCC 4 cut(s) 113, 263, 389, 548
Bso31I GGTCTC 1 cut(s) 492
BsoBI CYCGRG 1 cut(s) 123
Bsp143I GATC 3 cut(s) 412, 417, 521
Bsp1720I GCTNAGC 1 cut(s) 339
Bsp19I CCATGG 1 cut(s) 390
BspANI GGCC 4 cut(s) 113, 263, 389, 548
BspCNI CTCAG 3 cut(s) 331, 742, 776
BspFNI CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
BspLI GGNNCC 4 cut(s) 107, 114, 331, 547
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 2 cut(s) 311, 542
BspTNI GGTCTC 1 cut(s) 492
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 3 cut(s) 96, 596, 655
BssECI CCNNGG 6 cut(s) 108, 123, 282, 384, 390, 459
BssHII GCGCGC 1 cut(s) 205
BssMI GATC 3 cut(s) 412, 417, 521
BssNI GRCGYC 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 2 cut(s) 284, 386
Bst4CI ACNGT 2 cut(s) 70, 445
Bst6I CTCTTC 3 cut(s) 155, 311, 542
BstACI GRCGYC 1 cut(s) 510
BstC8I GCNNGC 3 cut(s) 135, 207, 672
BstDEI CTNAG 3 cut(s) 339, 750, 763
BstDSI CCRYGG 2 cut(s) 390, 459
BstF5I GGATG 1 cut(s) 373
BstFNI CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
BstHHI GCGC 3 cut(s) 181, 207, 209
BstKTI GATC 3 cut(s) 415, 420, 524
BstMAI GTCTC 2 cut(s) 104, 492
BstMBI GATC 3 cut(s) 412, 417, 521
BstMWI GCNNNNNNNGC 3 cut(s) 54, 71, 211
BstNI CCWGG 2 cut(s) 284, 386
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 7 cut(s) 108, 122, 123, 209, 234, 282, 384
BstUI CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
BstV1I GCAGC 4 cut(s) 86, 330, 661, 706
BsuRI GGCC 4 cut(s) 113, 263, 389, 548
BtgI CCRYGG 2 cut(s) 390, 459
BtgZI GCGATG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 373
Cac8I GCNNGC 3 cut(s) 135, 207, 672
CaiI CAGNNNCTG 1 cut(s) 102
CfoI GCGC 3 cut(s) 181, 207, 209
Cfr13I GGNCC 4 cut(s) 105, 112, 330, 546
Cfr9I CCCGGG 1 cut(s) 123
CseI GACGC 6 cut(s) 151, 180, 185, 220, 258, 518
Csp6I GTAC 2 cut(s) 83, 217
CviAII CATG 2 cut(s) 155, 391
CviQI GTAC 2 cut(s) 83, 217
DdeI CTNAG 3 cut(s) 339, 750, 763
DpnI GATC 3 cut(s) 414, 419, 523
DpnII GATC 3 cut(s) 412, 417, 521
DraI TTTAAA 1 cut(s) 586
DriI GACNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 3 cut(s) 155, 311, 542
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 3 cut(s) 155, 311, 542
Eco130I CCWWGG 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 105, 330
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 282, 384
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 2 cut(s) 158, 394
FaiI YATR 5 cut(s) 156, 392, 543, 666, 702
FaqI GGGAC 3 cut(s) 130, 218, 265
FatI CATG 2 cut(s) 154, 390
FauI CCCGC 2 cut(s) 123, 344
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 165
FokI GGATG 1 cut(s) 380
FspBI CTAG 1 cut(s) 684
GlaI GCGC 3 cut(s) 180, 206, 208
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 4 cut(s) 113, 263, 389, 548
HapII CCGG 6 cut(s) 110, 124, 210, 236, 428, 620
HgaI GACGC 6 cut(s) 151, 180, 185, 220, 258, 518
HhaI GCGC 3 cut(s) 181, 207, 209
Hin1I GRCGYC 1 cut(s) 510
Hin1II CATG 2 cut(s) 158, 394
Hin6I GCGC 3 cut(s) 179, 205, 207
HinP1I GCGC 3 cut(s) 179, 205, 207
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 3 cut(s) 143, 471, 497
HpaII CCGG 6 cut(s) 110, 124, 210, 236, 428, 620
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 4 cut(s) 175, 417, 492, 526
Hpy188III TCNNGA 4 cut(s) 103, 406, 468, 575
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 6 cut(s) 74, 167, 179, 274, 530, 644
HpyAV CCTTC 2 cut(s) 286, 355
HpyCH4III ACNGT 2 cut(s) 70, 445
HpyCH4IV ACGT 2 cut(s) 240, 528
HpyCH4V TGCA 2 cut(s) 670, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 71, 211
HpyF3I CTNAG 3 cut(s) 339, 750, 763
HpySE526I ACGT 2 cut(s) 240, 528
Hsp92I GRCGYC 1 cut(s) 510
Hsp92II CATG 2 cut(s) 158, 394
HspAI GCGC 3 cut(s) 179, 205, 207
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 3 cut(s) 412, 417, 521
LguI GCTCTTC 2 cut(s) 311, 542
LmnI GCTCC 2 cut(s) 231, 376
Lsp1109I GCAGC 4 cut(s) 86, 330, 661, 706
MaeI CTAG 1 cut(s) 684
MaeII ACGT 2 cut(s) 240, 528
MaeIII GTNAC 2 cut(s) 627, 757
MalI GATC 3 cut(s) 414, 419, 523
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 412, 417, 521
MboII GAAGA 4 cut(s) 142, 328, 370, 529
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 3 cut(s) 433, 450, 808
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 198, 549
Mox20I TGGCCA 1 cut(s) 263
MroXI GAANNNNTTC 3 cut(s) 401, 536, 809
MscI TGGCCA 1 cut(s) 263
MseI TTAA 1 cut(s) 585
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 6 cut(s) 110, 124, 210, 236, 428, 620
MspR9I CCNGG 7 cut(s) 110, 124, 125, 211, 236, 284, 386
MvaI CCWGG 2 cut(s) 284, 386
MvnI CGCG 6 cut(s) 162, 179, 191, 205, 207, 231
MwoI GCNNNNNNNGC 3 cut(s) 54, 71, 211
NciI CCSGG 5 cut(s) 110, 124, 125, 211, 236
NcoI CCATGG 1 cut(s) 390
NdeII GATC 3 cut(s) 412, 417, 521
NlaIII CATG 2 cut(s) 158, 394
NlaIV GGNNCC 4 cut(s) 107, 114, 331, 547
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 627
PauI GCGCGC 1 cut(s) 205
PciSI GCTCTTC 2 cut(s) 311, 542
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 171
PdmI GAANNNNTTC 3 cut(s) 401, 536, 809
PfeI GAWTC 2 cut(s) 471, 497
Pfl23II CGTACG 1 cut(s) 82
PflMI CCANNNNNTGG 2 cut(s) 102, 391
PfoI TCCNGGA 1 cut(s) 234
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PshAI GACNNNNGTC 1 cut(s) 96
PsiI TTATAA 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 282, 384
PspGI CCWGG 2 cut(s) 282, 384
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 4 cut(s) 107, 114, 331, 547
PspPI GGNCC 4 cut(s) 105, 112, 330, 546
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstNI CAGNNNCTG 1 cut(s) 102
PteI GCGCGC 1 cut(s) 205
RsaI GTAC 2 cut(s) 84, 218
RsaNI GTAC 2 cut(s) 83, 217
SalI GTCGAC 1 cut(s) 164
SapI GCTCTTC 2 cut(s) 311, 542
SaqAI TTAA 1 cut(s) 585
Sau3AI GATC 3 cut(s) 412, 417, 521
Sau96I GGNCC 4 cut(s) 105, 112, 330, 546
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 7 cut(s) 110, 124, 125, 211, 236, 284, 386
SinI GGWCC 2 cut(s) 105, 330
SmaI CCCGGG 1 cut(s) 125
Sse9I AATT 3 cut(s) 433, 450, 808
SspMI CTAG 1 cut(s) 684
StyD4I CCNGG 7 cut(s) 108, 122, 123, 209, 234, 282, 384
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 70, 445
TaiI ACGT 2 cut(s) 243, 531
TaqI TCGA 4 cut(s) 165, 303, 405, 420
TasI AATT 3 cut(s) 433, 450, 808
TauI GCSGC 8 cut(s) 18, 57, 60, 80, 140, 277, 290, 427
TfiI GAWTC 2 cut(s) 471, 497
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 4 cut(s) 74, 343, 674, 719
Tsp45I GTSAC 1 cut(s) 627
TspMI CCCGGG 1 cut(s) 123
Van91I CCANNNNNTGG 2 cut(s) 102, 391
VpaK11BI GGWCC 2 cut(s) 105, 330
XapI RAATTY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XmaI CCCGGG 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 3 cut(s) 401, 536, 809
XspI CTAG 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.