pycom13g02740

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002199
Physical Location & Seq
Reverse (-)
1887874 .. 1888605
732 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g02740.1

Sequence Viewer

Length: 546 bp
ATGTCACTTGCAATCTTCCAACCCGCATCGGTGCCCCTCCGCTCCCATGCACACCTTAGCTCCCCCCTCCCTCCGATCGTTGCCGCCGCCGTACGCCAAGACACCGCGCTCTGGACCCCGGCCCCTCTCTCCGAGATAGAACCGGCCGCAGAGTCGCTCTTCCACGTCCGCGTCGACCTGTCCGACGCCCCCGACCTCGCCTCGTCCCACACGCGAGCCGGCCAGTACCTCCAGCTTCGCGTCCCGGACGAGTCAAAGCCCTCGTTTCTGGCCATCGCGTCGCCTCCATCGTTGGCGGACGTAAAAGGCGTGTTTGAGTTTTTGGTGAAAAGCGTGGCAGGGTCCACGGCGGAGCTTCTGTGCAGGCTCAAGAGAGGGGACGTGGTGGAGCTCAGCCAGGTTATGGGGAAAGGCTTCGACATTGATCGGATCGAACCGCCGGAGAATTACCCTACGGTTCTCATATTCGCCACAGGATCTGGAATCAGTCCAATCCGGTCTCTGATCGAATCGGGGTTTAGCGCCGATAAGATCGATCCGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

182

Amino Acids

19.31

Weight (kDa)

5.14

Isoelectric Point (pI)

58.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 403
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 174
AccII CGCG 5 cut(s) 107, 171, 214, 240, 278
AclWI GGATC 3 cut(s) 437, 484, 530
AcoI YGGCCR 3 cut(s) 144, 220, 270
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 93, 227
AfiI CCNNNNNNNGG 2 cut(s) 111, 403
AjiI CACGTC 2 cut(s) 166, 382
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 4 cut(s) 60, 235, 355, 391
AluI AGCT 4 cut(s) 60, 235, 355, 391
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 3 cut(s) 437, 484, 530
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 4 cut(s) 120, 144, 220, 270
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 2 cut(s) 109, 524
AspS9I GGNCC 3 cut(s) 114, 121, 342
AsuC2I CCSGG 2 cut(s) 119, 245
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 2 cut(s) 114, 342
BaeGI GKGCMC 1 cut(s) 36
BalI TGGCCA 1 cut(s) 272
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 393
BccI CCATC 2 cut(s) 281, 295
BceAI ACGGC 3 cut(s) 74, 363, 526
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 2 cut(s) 119, 245
BcoDI GTCTC 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 525
BisI GCNGC 3 cut(s) 84, 87, 147
BlpI GCTNAGC 1 cut(s) 392
BlsI GCNGC 3 cut(s) 85, 88, 148
Bme1390I CCNGG 3 cut(s) 119, 245, 398
Bme18I GGWCC 2 cut(s) 114, 342
BmgBI CACGTC 2 cut(s) 166, 382
BmgT120I GGNCC 3 cut(s) 114, 121, 342
BmiI GGNNCC 4 cut(s) 33, 116, 123, 343
BmrFI CCNGG 3 cut(s) 119, 245, 398
BmsI GCATC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 56
Bpu1102I GCTNAGC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 2 cut(s) 119, 245
Bsa29I ATCGAT 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 117, 345
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 403
Bse118I RCCGGY 2 cut(s) 142, 218
Bse1I ACTGG 1 cut(s) 223
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 117, 345
BseLI CCNNNNNNNGG 2 cut(s) 111, 403
BseMII CTCAG 1 cut(s) 406
BseNI ACTGG 1 cut(s) 223
BseSI GKGCMC 1 cut(s) 36
BseX3I CGGCCG 1 cut(s) 144
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 5 cut(s) 107, 171, 214, 240, 278
Bsh1285I CGRYCG 2 cut(s) 78, 147
BshFI GGCC 4 cut(s) 122, 146, 222, 272
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 2 cut(s) 78, 147
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 6 cut(s) 119, 143, 219, 245, 440, 496
BsiWI CGTACG 1 cut(s) 91
BslFI GGGAC 3 cut(s) 190, 227, 392
BslI CCNNNNNNNGG 2 cut(s) 111, 403
BsmAI GTCTC 1 cut(s) 504
BsmFI GGGAC 3 cut(s) 190, 227, 392
BsnI GGCC 4 cut(s) 122, 146, 222, 272
Bso31I GGTCTC 1 cut(s) 504
Bsp1286I GDGCHC 2 cut(s) 36, 393
Bsp143I GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
Bsp1720I GCTNAGC 1 cut(s) 392
BspANI GGCC 4 cut(s) 122, 146, 222, 272
BspCNI CTCAG 1 cut(s) 405
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 5 cut(s) 107, 171, 214, 240, 278
BspLI GGNNCC 4 cut(s) 33, 116, 123, 343
BspPI GGATC 3 cut(s) 437, 484, 530
BspQI GCTCTTC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 504
BsrBI CCGCTC 1 cut(s) 42
BsrFI RCCGGY 2 cut(s) 142, 218
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 2 cut(s) 142, 218
BssECI CCNNGG 2 cut(s) 117, 345
BssMI GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 3 cut(s) 216, 220, 365
BstDEI CTNAG 2 cut(s) 56, 392
BstDSI CCRYGG 1 cut(s) 345
BstFNI CGCG 5 cut(s) 107, 171, 214, 240, 278
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 2 cut(s) 109, 524
BstKTI GATC 7 cut(s) 78, 427, 432, 479, 507, 534, 538
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
BstMCI CGRYCG 2 cut(s) 78, 147
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 3 cut(s) 117, 243, 396
BstSLI GKGCMC 1 cut(s) 36
BstUI CGCG 5 cut(s) 107, 171, 214, 240, 278
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BstZI CGGCCG 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 4 cut(s) 122, 146, 222, 272
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 259
BtrI CACGTC 2 cut(s) 166, 382
Cac8I GCNNGC 3 cut(s) 216, 220, 365
CaiI CAGNNNCTG 1 cut(s) 479
CfoI GCGC 2 cut(s) 109, 524
Cfr10I RCCGGY 2 cut(s) 142, 218
Cfr13I GGNCC 3 cut(s) 114, 121, 342
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 4 cut(s) 160, 194, 229, 267
Csp6I GTAC 2 cut(s) 92, 226
CviAII CATG 1 cut(s) 47
CviQI GTAC 2 cut(s) 92, 226
DdeI CTNAG 2 cut(s) 56, 392
DpnI GATC 7 cut(s) 77, 426, 431, 478, 506, 533, 537
DpnII GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
EaeI YGGCCR 3 cut(s) 144, 220, 270
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 3 cut(s) 311, 365, 528
Ecl136II GAGCTC 1 cut(s) 391
EclXI CGGCCG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 114, 342
Eco52I CGGCCG 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 393
FaeI CATG 1 cut(s) 50
FaiI YATR 3 cut(s) 48, 404, 464
FaqI GGGAC 3 cut(s) 190, 227, 392
FatI CATG 1 cut(s) 46
FauI CCCGC 1 cut(s) 31
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 84, 87, 147
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 84, 87, 147
GlaI GCGC 2 cut(s) 108, 523
GluI GCNGC 3 cut(s) 84, 87, 147
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 4 cut(s) 122, 146, 222, 272
HapII CCGG 6 cut(s) 119, 143, 219, 245, 440, 496
HgaI GACGC 4 cut(s) 160, 194, 229, 267
HhaI GCGC 2 cut(s) 109, 524
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 1 cut(s) 50
Hin6I GCGC 2 cut(s) 107, 522
HinP1I GCGC 2 cut(s) 107, 522
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 4 cut(s) 152, 251, 483, 509
HpaII CCGG 6 cut(s) 119, 143, 219, 245, 440, 496
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 2 cut(s) 175, 345
Hpy188I TCNGA 5 cut(s) 75, 133, 184, 429, 504
Hpy188III TCNNGA 3 cut(s) 112, 370, 480
Hpy8I GTNNAC 2 cut(s) 175, 345
Hpy99I CGWCG 3 cut(s) 176, 188, 283
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 3 cut(s) 165, 300, 381
HpyCH4V TGCA 3 cut(s) 11, 50, 363
HpyF3I CTNAG 2 cut(s) 56, 392
HpySE526I ACGT 3 cut(s) 165, 300, 381
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 1 cut(s) 50
HspAI GCGC 2 cut(s) 107, 522
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 4 cut(s) 47, 65, 352, 388
LweI GCATC 1 cut(s) 35
MaeII ACGT 3 cut(s) 165, 300, 381
MaeIII GTNAC 1 cut(s) 3
MalI GATC 7 cut(s) 77, 426, 431, 478, 506, 533, 537
MbiI CCGCTC 1 cut(s) 42
MboI GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
MboII GAAGA 2 cut(s) 7, 151
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 2 cut(s) 36, 393
MlsI TGGCCA 1 cut(s) 272
MluCI AATT 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 272
MlyI GAGTC 2 cut(s) 161, 260
MmeI TCCRAC 2 cut(s) 43, 207
Mox20I TGGCCA 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 218
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 272
MspI CCGG 6 cut(s) 119, 143, 219, 245, 440, 496
MspR9I CCNGG 3 cut(s) 119, 245, 398
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 5 cut(s) 107, 171, 214, 240, 278
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 2 cut(s) 119, 245
NdeII GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
NgoMIV GCCGGC 1 cut(s) 218
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 4 cut(s) 33, 116, 123, 343
NmuCI GTSAC 1 cut(s) 3
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 4 cut(s) 171, 180, 287, 306
PdiI GCCGGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 483, 509
Pfl23II CGTACG 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 403
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 3 cut(s) 85, 88, 148
Ple19I CGATCG 1 cut(s) 78
PleI GAGTC 2 cut(s) 160, 259
PpsI GAGTC 2 cut(s) 160, 259
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspLI CGTACG 1 cut(s) 91
PspN4I GGNNCC 4 cut(s) 33, 116, 123, 343
PspPI GGNCC 3 cut(s) 114, 121, 342
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 476
PvuI CGATCG 1 cut(s) 78
RsaI GTAC 2 cut(s) 93, 227
RsaNI GTAC 2 cut(s) 92, 226
SacI GAGCTC 1 cut(s) 393
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 164
SatI GCNGC 3 cut(s) 84, 87, 147
Sau3AI GATC 7 cut(s) 75, 424, 429, 476, 504, 531, 535
Sau96I GGNCC 3 cut(s) 114, 121, 342
SchI GAGTC 2 cut(s) 161, 260
ScrFI CCNGG 3 cut(s) 119, 245, 398
SduI GDGCHC 2 cut(s) 36, 393
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 2 cut(s) 114, 342
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 1 cut(s) 445
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 117, 243, 396
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 3 cut(s) 168, 303, 384
TaqI TCGA 5 cut(s) 174, 417, 432, 507, 534
TasI AATT 1 cut(s) 445
TauI GCSGC 3 cut(s) 86, 89, 149
TfiI GAWTC 2 cut(s) 483, 509
TseFI GTSAC 1 cut(s) 3
Tsp45I GTSAC 1 cut(s) 3
Van91I CCANNNNNTGG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 114, 342
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.