Rroxscaffold_5G00375280

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002199
Physical Location & Seq
Reverse (-)
56125916 .. 56128677
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375280.1

Sequence Viewer

Length: 873 bp
ATGTCATTTACAATCTGCCAACCCCATGCGCACCTTAGCCCACCCCTCCTCCCTCCCATGTCTATCCTACGCCGCCTCAGCCCCTCGCACCTAACCCTCCACCGTCACCGCCGCCGCCTCGCCACCGTCGCAGCCGCCGTACGCCAAGACACCACTCTCTGGACCCCGGCCCCGCTCTCCCGGGTTGAGCCTGCCGCCGAGTCCCTCTTCCACGTCCGCGTCGACCTCTCCGACGCGCCCGACCTCGCGTCCTCCTACACGTGCGCCGGGCAGTACCTCCAGCTCCGCGTCCCGGACGTTGAAAAGCCCTCGTTCTTGGCCATCGCGTCGCCGCCGGCCCTGGCTGCGAAGGGCGTGTTCGAGTTCTTGGTGAAGAGCGTGGAAGGGTCCATCGCTGAGCTGCTGTGCGGGCTGAAGAAGGGGGACGTTGTGGAGCTGACCCAGGCCATGGGGAAAGGCTTCGAAATCGATCGGATTGAGCCGCCGGAGAATTATCCTACGGTTCTAATTTTCGCCACGGGATCTGGAATCAGTCCAATCCGGTCTCTGATTGAATCAGGGTTTAACGCAGATAAGCGATCCGACGTGAAGCTCTTCTATGGGGCCAGAAACCTTGACAGAATGGCTTATCAGGATAGATTTAAAGATTGGGAAGCTTCAGGTGTAAAGATAGTTCCTGTATTGTCACAACCACACGACGGCTGGACTGGACAAAGTGGTTATGTGCAGGCTGCTTTTTCTAGAGCCAAGCAAGTTTATAACCCTCTCTCCACCGCTGCTGTTCTCTGTGGGCAGAAACAGATGGCTGAGGAAGTTACCTCAATTCTTTTAGCAGATGGAGTTGCAAGTGAAAAAATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

290

Amino Acids

31.68

Weight (kDa)

8.3

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 172 - 272 2e-16 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
Acc16I TGCGCA 1 cut(s) 30
AccB7I CCANNNNNTGG 2 cut(s) 159, 448
AccBSI CCGCTC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 222
AccII CGCG 5 cut(s) 219, 236, 248, 288, 326
AclWI GGATC 2 cut(s) 529, 573
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 865
AcuI CTGAAG 2 cut(s) 434, 642
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 2 cut(s) 141, 275
AfiI CCNNNNNNNGG 4 cut(s) 159, 292, 448, 698
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 2 cut(s) 302, 554
AhdI GACNNNNNGTC 1 cut(s) 247
AjiI CACGTC 2 cut(s) 214, 586
AjnI CCWGG 2 cut(s) 339, 441
AluBI AGCT 5 cut(s) 283, 400, 436, 592, 656
AluI AGCT 5 cut(s) 283, 400, 436, 592, 656
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 2 cut(s) 529, 573
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 5 cut(s) 168, 318, 336, 444, 603
ApeKI GCWGC 5 cut(s) 131, 344, 400, 731, 776
ApoI RAATTY 1 cut(s) 865
Asp700I GAANNNNTTC 3 cut(s) 458, 593, 866
AspLEI GCGC 3 cut(s) 31, 238, 266
AspS9I GGNCC 5 cut(s) 162, 169, 337, 387, 603
AsuC2I CCSGG 5 cut(s) 167, 181, 182, 268, 293
AsuHPI GGTGA 2 cut(s) 98, 382
AsuII TTCGAA 1 cut(s) 462
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 2 cut(s) 162, 387
BalI TGGCCA 1 cut(s) 320
BbrPI CACGTG 1 cut(s) 261
BbvCI CCTCAGC 2 cut(s) 77, 807
BbvI GCAGC 5 cut(s) 143, 331, 387, 718, 763
BccI CCATC 4 cut(s) 329, 398, 796, 830
BceAI ACGGC 2 cut(s) 122, 715
BciT130I CCWGG 2 cut(s) 341, 443
BcnI CCSGG 5 cut(s) 167, 181, 182, 268, 293
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 1 cut(s) 741
BlpI GCTNAGC 1 cut(s) 396
Bme1390I CCNGG 7 cut(s) 167, 181, 182, 268, 293, 341, 443
Bme18I GGWCC 2 cut(s) 162, 387
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 2 cut(s) 214, 586
BmgT120I GGNCC 5 cut(s) 162, 169, 337, 387, 603
BmiI GGNNCC 4 cut(s) 164, 171, 388, 604
BmrFI CCNGG 7 cut(s) 167, 181, 182, 268, 293, 341, 443
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 3 cut(s) 35, 77, 807
Bpu1102I GCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 462
BpuMI CCSGG 5 cut(s) 167, 181, 182, 268, 293
Bsa29I ATCGAT 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 6 cut(s) 165, 180, 339, 441, 447, 516
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 752, 782
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 292, 448, 698
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 712
BseBI CCWGG 2 cut(s) 341, 443
BseCI ATCGAT 1 cut(s) 468
BseDI CCNNGG 6 cut(s) 165, 180, 339, 441, 447, 516
BseLI CCNNNNNNNGG 4 cut(s) 159, 292, 448, 698
BseMII CTCAG 3 cut(s) 91, 387, 798
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 5 cut(s) 143, 331, 387, 718, 763
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 5 cut(s) 219, 236, 248, 288, 326
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 5 cut(s) 170, 320, 338, 446, 605
BshVI ATCGAT 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 7 cut(s) 167, 181, 267, 293, 335, 485, 541
BsiWI CGTACG 1 cut(s) 139
BslFI GGGAC 3 cut(s) 187, 275, 437
BslI CCNNNNNNNGG 4 cut(s) 159, 292, 448, 698
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 3 cut(s) 187, 275, 437
BsnI GGCC 5 cut(s) 170, 320, 338, 446, 605
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 180
Bsp119I TTCGAA 1 cut(s) 462
Bsp143I GATC 3 cut(s) 469, 521, 578
Bsp1720I GCTNAGC 1 cut(s) 396
Bsp19I CCATGG 1 cut(s) 447
BspANI GGCC 5 cut(s) 170, 320, 338, 446, 605
BspCNI CTCAG 3 cut(s) 90, 388, 799
BspDI ATCGAT 1 cut(s) 468
BspFNI CGCG 5 cut(s) 219, 236, 248, 288, 326
BspLI GGNNCC 4 cut(s) 164, 171, 388, 604
BspPI GGATC 2 cut(s) 529, 573
BspQI GCTCTTC 2 cut(s) 368, 599
BspT104I TTCGAA 1 cut(s) 462
BspTNI GGTCTC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 6 cut(s) 165, 180, 339, 441, 447, 516
BssMI GATC 3 cut(s) 469, 521, 578
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 2 cut(s) 341, 443
Bst4CI ACNGT 3 cut(s) 104, 127, 502
Bst6I CTCTTC 3 cut(s) 212, 368, 599
BstBAI YACGTR 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 462
BstC8I GCNNGC 4 cut(s) 192, 336, 410, 729
BstDEI CTNAG 4 cut(s) 35, 77, 396, 807
BstDSI CCRYGG 2 cut(s) 447, 516
BstFNI CGCG 5 cut(s) 219, 236, 248, 288, 326
BstHHI GCGC 3 cut(s) 31, 238, 266
BstKTI GATC 3 cut(s) 472, 524, 581
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 3 cut(s) 469, 521, 578
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 4 cut(s) 78, 128, 344, 409
BstNI CCWGG 2 cut(s) 341, 443
BstSCI CCNGG 7 cut(s) 165, 179, 180, 266, 291, 339, 441
BstUI CGCG 5 cut(s) 219, 236, 248, 288, 326
BstV1I GCAGC 5 cut(s) 143, 331, 387, 718, 763
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 468
BsuRI GGCC 5 cut(s) 170, 320, 338, 446, 605
BsuTUI ATCGAT 1 cut(s) 468
BtgI CCRYGG 2 cut(s) 447, 516
BtgZI GCGATG 2 cut(s) 307, 376
BtrI CACGTC 2 cut(s) 214, 586
Cac8I GCNNGC 4 cut(s) 192, 336, 410, 729
CfoI GCGC 3 cut(s) 31, 238, 266
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 5 cut(s) 162, 169, 337, 387, 603
Cfr9I CCCGGG 1 cut(s) 180
ClaI ATCGAT 1 cut(s) 468
CseI GACGC 5 cut(s) 208, 237, 242, 277, 315
Csp6I GTAC 2 cut(s) 140, 274
CviAII CATG 3 cut(s) 26, 58, 448
CviQI GTAC 2 cut(s) 140, 274
DdeI CTNAG 4 cut(s) 35, 77, 396, 807
DpnI GATC 3 cut(s) 471, 523, 580
DpnII GATC 3 cut(s) 469, 521, 578
DraI TTTAAA 1 cut(s) 643
DriI GACNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 3 cut(s) 212, 368, 599
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 3 cut(s) 212, 368, 599
Eco130I CCWWGG 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 162, 387
Eco57I CTGAAG 2 cut(s) 434, 642
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 339, 441
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 3 cut(s) 29, 61, 451
FaiI YATR 6 cut(s) 27, 59, 449, 600, 723, 759
FaqI GGGAC 3 cut(s) 187, 275, 437
FatI CATG 3 cut(s) 25, 57, 447
FauI CCCGC 2 cut(s) 180, 401
FblI GTMKAC 1 cut(s) 222
FspAI RTGCGCAY 1 cut(s) 30
FspBI CTAG 1 cut(s) 741
FspI TGCGCA 1 cut(s) 30
GlaI GCGC 3 cut(s) 30, 237, 265
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 5 cut(s) 170, 320, 338, 446, 605
HapII CCGG 7 cut(s) 167, 181, 267, 293, 335, 485, 541
HgaI GACGC 5 cut(s) 208, 237, 242, 277, 315
HhaI GCGC 3 cut(s) 31, 238, 266
Hin1II CATG 3 cut(s) 29, 61, 451
Hin6I GCGC 3 cut(s) 29, 236, 264
HinP1I GCGC 3 cut(s) 29, 236, 264
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 3 cut(s) 200, 528, 554
HpaII CCGG 7 cut(s) 167, 181, 267, 293, 335, 485, 541
HphI GGTGA 2 cut(s) 98, 382
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 4 cut(s) 232, 474, 549, 583
Hpy188III TCNNGA 4 cut(s) 160, 525, 632, 741
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 6 cut(s) 131, 224, 236, 331, 587, 701
HpyAV CCTTC 3 cut(s) 343, 377, 412
HpyCH4III ACNGT 3 cut(s) 104, 127, 502
HpyCH4IV ACGT 5 cut(s) 213, 260, 297, 426, 585
HpyCH4V TGCA 2 cut(s) 727, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 128, 344, 409
HpyF3I CTNAG 4 cut(s) 35, 77, 396, 807
HpySE526I ACGT 5 cut(s) 213, 260, 297, 426, 585
Hsp92II CATG 3 cut(s) 29, 61, 451
HspAI GCGC 3 cut(s) 29, 236, 264
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 3 cut(s) 469, 521, 578
LguI GCTCTTC 2 cut(s) 368, 599
LmnI GCTCC 2 cut(s) 288, 433
Lsp1109I GCAGC 5 cut(s) 143, 331, 387, 718, 763
MaeI CTAG 1 cut(s) 741
MaeII ACGT 5 cut(s) 213, 260, 297, 426, 585
MaeIII GTNAC 3 cut(s) 104, 684, 814
MalI GATC 3 cut(s) 471, 523, 580
MbiI CCGCTC 1 cut(s) 175
MboI GATC 3 cut(s) 469, 521, 578
MboII GAAGA 4 cut(s) 199, 385, 427, 586
MflI RGATCY 1 cut(s) 521
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 4 cut(s) 490, 507, 822, 865
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 2 cut(s) 255, 606
Mox20I TGGCCA 1 cut(s) 320
MroNI GCCGGC 1 cut(s) 334
MroXI GAANNNNTTC 3 cut(s) 458, 593, 866
MscI TGGCCA 1 cut(s) 320
MseI TTAA 2 cut(s) 564, 642
Msp20I TGGCCA 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 7 cut(s) 167, 181, 267, 293, 335, 485, 541
MspR9I CCNGG 7 cut(s) 167, 181, 182, 268, 293, 341, 443
MvaI CCWGG 2 cut(s) 341, 443
MvnI CGCG 5 cut(s) 219, 236, 248, 288, 326
MwoI GCNNNNNNNGC 4 cut(s) 78, 128, 344, 409
NaeI GCCGGC 1 cut(s) 336
NciI CCSGG 5 cut(s) 167, 181, 182, 268, 293
NcoI CCATGG 1 cut(s) 447
NdeII GATC 3 cut(s) 469, 521, 578
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 3 cut(s) 29, 61, 451
NlaIV GGNNCC 4 cut(s) 164, 171, 388, 604
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 104, 684
NsbI TGCGCA 1 cut(s) 30
NspV TTCGAA 1 cut(s) 462
PciSI GCTCTTC 2 cut(s) 368, 599
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 219, 228
PdiI GCCGGC 1 cut(s) 336
PdmI GAANNNNTTC 3 cut(s) 458, 593, 866
PfeI GAWTC 2 cut(s) 528, 554
Pfl23II CGTACG 1 cut(s) 139
PflMI CCANNNNNTGG 2 cut(s) 159, 448
PfoI TCCNGGA 1 cut(s) 291
Ple19I CGATCG 1 cut(s) 472
PleI GAGTC 1 cut(s) 208
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 1 cut(s) 208
Ppu21I YACGTR 1 cut(s) 261
PsiI TTATAA 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 339, 441
PspCI CACGTG 1 cut(s) 261
PspGI CCWGG 2 cut(s) 339, 441
PspLI CGTACG 1 cut(s) 139
PspN4I GGNNCC 4 cut(s) 164, 171, 388, 604
PspPI GGNCC 5 cut(s) 162, 169, 337, 387, 603
PsrI GAACNNNNNNTAC 2 cut(s) 657, 689
PsuI RGATCY 1 cut(s) 521
PvuI CGATCG 1 cut(s) 472
RsaI GTAC 2 cut(s) 141, 275
RsaNI GTAC 2 cut(s) 140, 274
SalI GTCGAC 1 cut(s) 221
SapI GCTCTTC 2 cut(s) 368, 599
SaqAI TTAA 2 cut(s) 564, 642
Sau3AI GATC 3 cut(s) 469, 521, 578
Sau96I GGNCC 5 cut(s) 162, 169, 337, 387, 603
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 7 cut(s) 167, 181, 182, 268, 293, 341, 443
SfuI TTCGAA 1 cut(s) 462
SinI GGWCC 2 cut(s) 162, 387
SmaI CCCGGG 1 cut(s) 182
Sse9I AATT 4 cut(s) 490, 507, 822, 865
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 7 cut(s) 165, 179, 180, 266, 291, 339, 441
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 104, 127, 502
TaiI ACGT 5 cut(s) 216, 263, 300, 429, 588
TaqI TCGA 4 cut(s) 222, 360, 462, 468
TasI AATT 4 cut(s) 490, 507, 822, 865
TauI GCSGC 7 cut(s) 75, 114, 117, 137, 197, 334, 484
TfiI GAWTC 2 cut(s) 528, 554
Tru1I TTAA 2 cut(s) 564, 642
Tru9I TTAA 2 cut(s) 564, 642
TseFI GTSAC 2 cut(s) 104, 684
TseI GCWGC 5 cut(s) 131, 344, 400, 731, 776
Tsp45I GTSAC 2 cut(s) 104, 684
TspMI CCCGGG 1 cut(s) 180
Van91I CCANNNNNTGG 2 cut(s) 159, 448
VpaK11BI GGWCC 2 cut(s) 162, 387
XapI RAATTY 1 cut(s) 865
XbaI TCTAGA 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 699
XmaI CCCGGG 1 cut(s) 180
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 3 cut(s) 458, 593, 866
XspI CTAG 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.