MD16G1033500.v1.1

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002199
Physical Location & Seq
Reverse (-)
2423209 .. 2426003
2795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1033500.v1.1.491

Sequence Viewer

Length: 888 bp
ATGTCACTCGCAATCTTCCAACCCGCATCGGTCACCCTCCACTCCCATGCGCACCTTAGCTCCCCCCTCCACCCCATGTCTATCCTACGCCGCCTCAACCCCCACCACCTAAAACTACGCCGCCACCGCTTCGCCACCGTCGCCGCCGCCGTACGCCAAGACACCGCCCTCTGGACCCCCGCCCCGCTCTCCGAGATAGAACCGGCCGCCGAGTCGCTCTTCCACGTCCGCGTCGACCTGTCCGACGCCCCCGACCTCGCCTCGTCCCACACGCGAGCCGGCCAGTACCTCCAGCTTCGCGTCCCGGACGAGTCGAAGCCCTCGTTTCTGGCCATCGCGTCGCCGCCATCGTTGGCGTCCACAAAAGGGGTGTTTGAGTTTTTGGTGAAGAGCGTGGCCGGGTCCACGGCGGAGCTTCTGTGCGGGCTCAAGAGAGGGGCCGTGGTGGAGCTCAGCCAGGTCATGGGAAAAGGCTTCGACGTTGAACGGATCGAACCGCCGGAAAATTACCCTACGGTTCTAATTTTCGCCACGGGATCTGGAATCAGTCCAATCCGGTCTCTGATCGAATCGGGGTTTAGCGCCGATAAGAGATCCGCCGTTAAGCTATTTTATGGCGCCAGAAACCTTGACAGAATGGCGTATCAGGATAGATTTAAGGACTGGGAATCTTCAGGTGTTGAGATTGTGCCGGTACTATCACAACCCCACGGCGGTTGGACGGGACAAAGCGGCTATGTGCAGGCTGCCTTTTCCAGAGCCAAGCAAATTGATAACCCTCCCTCTACAGCTGCTGTTTTATGTGGGCAGAAGCAGATGACTGAGGAGGTTACCTCAATTCTTGTAGCAGATGGTGTATCAACTGAAAACATACTGAAGAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

296

Amino Acids

32.01

Weight (kDa)

7.86

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 177 - 277 4.2e-16 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 617
AccB7I CCANNNNNTGG 1 cut(s) 463
AccBSI CCGCTC 1 cut(s) 187
AccI GTMKAC 1 cut(s) 234
AccII CGCG 4 cut(s) 231, 274, 300, 338
AclWI GGATC 3 cut(s) 497, 544, 588
AcoI YGGCCR 4 cut(s) 204, 280, 330, 396
AcsI RAATTY 1 cut(s) 880
AcuI CTGAAG 1 cut(s) 657
AcyI GRCGYC 3 cut(s) 246, 356, 618
AfaI GTAC 3 cut(s) 153, 287, 696
AfiI CCNNNNNNNGG 4 cut(s) 171, 366, 463, 713
AgsI TTSAA 1 cut(s) 485
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 6 cut(s) 60, 295, 415, 451, 607, 791
AluI AGCT 6 cut(s) 60, 295, 415, 451, 607, 791
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 3 cut(s) 497, 544, 588
AlwNI CAGNNNCTG 1 cut(s) 794
AoxI GGCC 5 cut(s) 204, 280, 330, 396, 438
ApeKI GCWGC 2 cut(s) 746, 791
ApoI RAATTY 1 cut(s) 880
Asp700I GAANNNNTTC 1 cut(s) 881
AspLEI GCGC 3 cut(s) 52, 584, 620
AspS9I GGNCC 3 cut(s) 174, 402, 438
AsuC2I CCSGG 2 cut(s) 305, 400
AsuHPI GGTGA 2 cut(s) 25, 397
AvaII GGWCC 2 cut(s) 174, 402
BalI TGGCCA 1 cut(s) 332
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 429, 453
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 733, 778
BccI CCATC 3 cut(s) 341, 355, 845
BceAI ACGGC 5 cut(s) 134, 423, 425, 584, 727
BciT130I CCWGG 1 cut(s) 458
BcnI CCSGG 2 cut(s) 305, 400
BcoDI GTCTC 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 2 cut(s) 585, 621
BisI GCNGC 9 cut(s) 91, 121, 144, 147, 207, 344, 733, 747, 792
BlpI GCTNAGC 1 cut(s) 452
BlsI GCNGC 9 cut(s) 92, 122, 145, 148, 208, 345, 734, 748, 793
Bme1390I CCNGG 3 cut(s) 305, 400, 458
Bme18I GGWCC 2 cut(s) 174, 402
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 174, 402, 438
BmiI GGNNCC 4 cut(s) 176, 403, 439, 619
BmrFI CCNGG 3 cut(s) 305, 400, 458
BmrI ACTGGG 1 cut(s) 673
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 673
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 56
Bpu1102I GCTNAGC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 2 cut(s) 305, 400
BsaHI GRCGYC 3 cut(s) 246, 356, 618
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 4 cut(s) 405, 441, 531, 709
BsaWI WCCGGW 1 cut(s) 555
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 404, 434
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 366, 463, 713
Bse118I RCCGGY 3 cut(s) 202, 278, 691
Bse1I ACTGG 2 cut(s) 283, 668
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 4 cut(s) 405, 441, 531, 709
BseLI CCNNNNNNNGG 4 cut(s) 171, 366, 463, 713
BseMII CTCAG 2 cut(s) 466, 813
BseNI ACTGG 2 cut(s) 283, 668
BseRI GAGGAG 1 cut(s) 839
BseX3I CGGCCG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 733, 778
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 4 cut(s) 231, 274, 300, 338
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 5 cut(s) 206, 282, 332, 398, 440
BshNI GGYRCC 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 7 cut(s) 203, 279, 305, 399, 500, 556, 692
BsiWI CGTACG 1 cut(s) 151
BslFI GGGAC 3 cut(s) 250, 287, 738
BslI CCNNNNNNNGG 4 cut(s) 171, 366, 463, 713
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 3 cut(s) 250, 287, 738
BsnI GGCC 5 cut(s) 206, 282, 332, 398, 440
Bso31I GGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 2 cut(s) 429, 453
Bsp143I GATC 4 cut(s) 489, 536, 564, 593
Bsp1720I GCTNAGC 1 cut(s) 452
BspANI GGCC 5 cut(s) 206, 282, 332, 398, 440
BspCNI CTCAG 2 cut(s) 465, 814
BspFNI CGCG 4 cut(s) 231, 274, 300, 338
BspLI GGNNCC 4 cut(s) 176, 403, 439, 619
BspPI GGATC 3 cut(s) 497, 544, 588
BspQI GCTCTTC 2 cut(s) 224, 383
BspT107I GGYRCC 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 187
BsrFI RCCGGY 3 cut(s) 202, 278, 691
BsrI ACTGG 2 cut(s) 283, 668
BssAI RCCGGY 3 cut(s) 202, 278, 691
BssECI CCNNGG 4 cut(s) 405, 441, 531, 709
BssMI GATC 4 cut(s) 489, 536, 564, 593
BssNI GRCGYC 3 cut(s) 246, 356, 618
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 2 cut(s) 139, 517
Bst6I CTCTTC 2 cut(s) 224, 383
BstACI GRCGYC 3 cut(s) 246, 356, 618
BstC8I GCNNGC 4 cut(s) 276, 280, 425, 744
BstDEI CTNAG 3 cut(s) 56, 452, 822
BstDSI CCRYGG 4 cut(s) 405, 441, 531, 709
BstEII GGTNACC 2 cut(s) 31, 829
BstFNI CGCG 4 cut(s) 231, 274, 300, 338
BstH2I RGCGCY 2 cut(s) 585, 621
BstHHI GCGC 3 cut(s) 52, 584, 620
BstKTI GATC 4 cut(s) 492, 539, 567, 596
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 4 cut(s) 489, 536, 564, 593
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 2 cut(s) 126, 140
BstNI CCWGG 1 cut(s) 458
BstPI GGTNACC 2 cut(s) 31, 829
BstSCI CCNGG 3 cut(s) 303, 398, 456
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 4 cut(s) 231, 274, 300, 338
BstV1I GCAGC 2 cut(s) 733, 778
BstX2I RGATCY 2 cut(s) 536, 593
BstYI RGATCY 2 cut(s) 536, 593
BstZI CGGCCG 1 cut(s) 204
BsuRI GGCC 5 cut(s) 206, 282, 332, 398, 440
BtgI CCRYGG 4 cut(s) 405, 441, 531, 709
BtgZI GCGATG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 226
Cac8I GCNNGC 4 cut(s) 276, 280, 425, 744
CaiI CAGNNNCTG 1 cut(s) 794
CfoI GCGC 3 cut(s) 52, 584, 620
Cfr10I RCCGGY 3 cut(s) 202, 278, 691
Cfr13I GGNCC 3 cut(s) 174, 402, 438
CseI GACGC 5 cut(s) 220, 254, 289, 327, 345
Csp6I GTAC 3 cut(s) 152, 286, 695
CspCI CAANNNNNGTGG 2 cut(s) 698, 733
CviAII CATG 3 cut(s) 47, 76, 463
CviQI GTAC 3 cut(s) 152, 286, 695
DdeI CTNAG 3 cut(s) 56, 452, 822
DinI GGCGCC 1 cut(s) 619
DpnI GATC 4 cut(s) 491, 538, 566, 595
DpnII GATC 4 cut(s) 489, 536, 564, 593
EaeI YGGCCR 4 cut(s) 204, 280, 330, 396
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 2 cut(s) 224, 383
EarI CTCTTC 2 cut(s) 224, 383
EciI GGCGGA 2 cut(s) 425, 586
Ecl136II GAGCTC 1 cut(s) 451
EclXI CGGCCG 1 cut(s) 204
Eco24I GRGCYC 2 cut(s) 429, 453
Eco31I GGTCTC 1 cut(s) 564
Eco47I GGWCC 2 cut(s) 174, 402
Eco52I CGGCCG 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 657
Eco91I GGTNACC 2 cut(s) 31, 829
EcoICRI GAGCTC 1 cut(s) 451
EcoO65I GGTNACC 2 cut(s) 31, 829
EcoRII CCWGG 1 cut(s) 456
EcoT38I GRGCYC 2 cut(s) 429, 453
EgeI GGCGCC 1 cut(s) 619
EheI GGCGCC 1 cut(s) 619
FaeI CATG 3 cut(s) 50, 79, 466
FaiI YATR 7 cut(s) 48, 77, 464, 615, 738, 802, 872
FaqI GGGAC 3 cut(s) 250, 287, 738
FatI CATG 3 cut(s) 46, 75, 462
FauI CCCGC 4 cut(s) 31, 187, 192, 416
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 9 cut(s) 91, 121, 144, 147, 207, 344, 733, 747, 792
FriOI GRGCYC 2 cut(s) 429, 453
Fsp4HI GCNGC 9 cut(s) 91, 121, 144, 147, 207, 344, 733, 747, 792
FspAI RTGCGCAY 1 cut(s) 51
FspI TGCGCA 1 cut(s) 51
GlaI GCGC 3 cut(s) 51, 583, 619
GluI GCNGC 9 cut(s) 91, 121, 144, 147, 207, 344, 733, 747, 792
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 585, 621
HaeIII GGCC 5 cut(s) 206, 282, 332, 398, 440
HapII CCGG 7 cut(s) 203, 279, 305, 399, 500, 556, 692
HgaI GACGC 5 cut(s) 220, 254, 289, 327, 345
HhaI GCGC 3 cut(s) 52, 584, 620
Hin1I GRCGYC 3 cut(s) 246, 356, 618
Hin1II CATG 3 cut(s) 50, 79, 466
Hin6I GCGC 3 cut(s) 50, 582, 618
HinP1I GCGC 3 cut(s) 50, 582, 618
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 5 cut(s) 212, 311, 543, 569, 668
HpaII CCGG 7 cut(s) 203, 279, 305, 399, 500, 556, 692
HphI GGTGA 2 cut(s) 25, 397
Hpy166II GTNNAC 3 cut(s) 235, 360, 405
Hpy188I TCNGA 4 cut(s) 193, 244, 564, 887
Hpy188III TCNNGA 5 cut(s) 172, 430, 540, 647, 756
Hpy8I GTNNAC 3 cut(s) 235, 360, 405
Hpy99I CGWCG 5 cut(s) 143, 236, 248, 343, 482
HpyCH4III ACNGT 2 cut(s) 139, 517
HpyCH4IV ACGT 2 cut(s) 225, 480
HpyCH4V TGCA 1 cut(s) 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 140
HpyF3I CTNAG 3 cut(s) 56, 452, 822
HpySE526I ACGT 2 cut(s) 225, 480
Hsp92I GRCGYC 3 cut(s) 246, 356, 618
Hsp92II CATG 3 cut(s) 50, 79, 466
HspAI GCGC 3 cut(s) 50, 582, 618
KasI GGCGCC 1 cut(s) 617
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 4 cut(s) 489, 536, 564, 593
LguI GCTCTTC 2 cut(s) 224, 383
LmnI GCTCC 3 cut(s) 65, 412, 448
Lsp1109I GCAGC 2 cut(s) 733, 778
LweI GCATC 1 cut(s) 35
MaeII ACGT 2 cut(s) 225, 480
MaeIII GTNAC 3 cut(s) 3, 31, 829
MalI GATC 4 cut(s) 491, 538, 566, 595
MbiI CCGCTC 1 cut(s) 187
MboI GATC 4 cut(s) 489, 536, 564, 593
MboII GAAGA 4 cut(s) 7, 211, 400, 663
MflI RGATCY 2 cut(s) 536, 593
MhlI GDGCHC 2 cut(s) 429, 453
MlsI TGGCCA 1 cut(s) 332
MluCI AATT 5 cut(s) 505, 522, 768, 837, 880
MluNI TGGCCA 1 cut(s) 332
Mly113I GGCGCC 1 cut(s) 618
MlyI GAGTC 2 cut(s) 221, 320
MmeI TCCRAC 3 cut(s) 43, 267, 698
Mox20I TGGCCA 1 cut(s) 332
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 881
MscI TGGCCA 1 cut(s) 332
MseI TTAA 2 cut(s) 603, 657
MslI CAYNNNNRTG 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 7 cut(s) 203, 279, 305, 399, 500, 556, 692
MspR9I CCNGG 3 cut(s) 305, 400, 458
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 4 cut(s) 231, 274, 300, 338
MwoI GCNNNNNNNGC 2 cut(s) 126, 140
NaeI GCCGGC 1 cut(s) 280
NarI GGCGCC 1 cut(s) 618
NciI CCSGG 2 cut(s) 305, 400
NdeII GATC 4 cut(s) 489, 536, 564, 593
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 3 cut(s) 50, 79, 466
NlaIV GGNNCC 4 cut(s) 176, 403, 439, 619
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 2 cut(s) 3, 31
NsbI TGCGCA 1 cut(s) 51
PciSI GCTCTTC 2 cut(s) 224, 383
PcsI WCGNNNNNNNCGW 5 cut(s) 147, 231, 240, 320, 347
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 3 cut(s) 543, 569, 668
Pfl23II CGTACG 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 463
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 9 cut(s) 92, 122, 145, 148, 208, 345, 734, 748, 793
PleI GAGTC 2 cut(s) 220, 319
PluTI GGCGCC 1 cut(s) 621
PpsI GAGTC 2 cut(s) 220, 319
Psp124BI GAGCTC 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 456
PspEI GGTNACC 2 cut(s) 31, 829
PspGI CCWGG 1 cut(s) 456
PspLI CGTACG 1 cut(s) 151
PspN4I GGNNCC 4 cut(s) 176, 403, 439, 619
PspPI GGNCC 3 cut(s) 174, 402, 438
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 2 cut(s) 536, 593
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 3 cut(s) 153, 287, 696
RsaNI GTAC 3 cut(s) 152, 286, 695
RseI CAYNNNNRTG 1 cut(s) 45
SacI GAGCTC 1 cut(s) 453
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 2 cut(s) 224, 383
SaqAI TTAA 2 cut(s) 603, 657
SatI GCNGC 9 cut(s) 91, 121, 144, 147, 207, 344, 733, 747, 792
Sau3AI GATC 4 cut(s) 489, 536, 564, 593
Sau96I GGNCC 3 cut(s) 174, 402, 438
SchI GAGTC 2 cut(s) 221, 320
ScrFI CCNGG 3 cut(s) 305, 400, 458
SduI GDGCHC 2 cut(s) 429, 453
SfaNI GCATC 1 cut(s) 35
SfcI CTRYAG 1 cut(s) 786
SfoI GGCGCC 1 cut(s) 619
SinI GGWCC 2 cut(s) 174, 402
SmiMI CAYNNNNRTG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 5 cut(s) 505, 522, 768, 837, 880
SspDI GGCGCC 1 cut(s) 617
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 3 cut(s) 303, 398, 456
TaaI ACNGT 2 cut(s) 139, 517
TaiI ACGT 2 cut(s) 228, 483
TaqI TCGA 5 cut(s) 234, 314, 477, 492, 567
TaqII GACCGA 1 cut(s) 19
TasI AATT 5 cut(s) 505, 522, 768, 837, 880
TauI GCSGC 7 cut(s) 93, 123, 146, 149, 209, 346, 735
TfiI GAWTC 3 cut(s) 543, 569, 668
Tru1I TTAA 2 cut(s) 603, 657
Tru9I TTAA 2 cut(s) 603, 657
TseFI GTSAC 2 cut(s) 3, 31
TseI GCWGC 2 cut(s) 746, 791
Tsp45I GTSAC 2 cut(s) 3, 31
TspGWI ACGGA 1 cut(s) 502
Van91I CCANNNNNTGG 1 cut(s) 463
VpaK11BI GGWCC 2 cut(s) 174, 402
XapI RAATTY 1 cut(s) 880
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.