FvH4_4g26280

Fruit protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002199
Physical Location & Seq
Reverse (-)
27740574 .. 27743618
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26280.t1

Sequence Viewer

Length: 879 bp
ATGTCATTTACAATCTCCCACGCTCCGTTGGCGCCTCATGCGCACCTTAGCCCCTCCCTCCCTCCCATGTCTATCCTACGCCGCCTCAACCCCTCCCGCCTAACCCTCCGCCTCCACCGCCGCCGCCTCGCCACCGTCGCCGCCGCCGTACGCCAGGACACCACTCTCTGGACCCCGGCCCCGCTCTCCCGCGTTGAGCCCGCCGCCGAGTCGCTGTTCCACGTCCGCGTCGACCTGTCCGACGCGCCCGACCTCGCGTCCTCCTACACGCGCCCCGGGCAGTACCTCCAGCTGCGCGTCCCGGACGTCGAGAAGCCCTCGTTCCTGGCCATCGCGTCTCCGCCGGCGCTGGCGGCGAAGGGCGTGTTTGAGTTTTTGGTGAAGAGCGTGGAGGGGTCGATTGCGGAGCTGCTGTGCGGGATGAAGAAGGGAGACGTGGTGGAGCTGACGCAGGCTATGGGGAAAGGCTTCGAAATCGATCAGATCCCGCCGGAGAATTATCCGACGGTTTTGATTTTCGCGACGGGATCTGGAATCAGCCCAATTCGGTCTCTGATTGAATCAGGGTTTAACGCCGATAAGCGATCCGACGTGAAGCTCTTCTATGGGGCCAGAAACCTGGACAGAATGGCTTATCAGGATAGGTTTAAAGACTGGGAAGCTTCAGGTGTAAACATAGTTCCTGTATTATCACAACCACAAGACGGTTGGACTGGACAAAGCGGTTATGTACAGGCTGCTTTTTCTAGAGCCAAGCAAGCTTATAATCCTCTTTCCACCGCTGCTGTTCTCTGTGGGCAGAAACAGATGGCTGAGGAAGTTACCTCCATTCTTTTAGCTGATGGAGTTGCAAGTGAAAAGATACTGAAGAATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

293

Amino Acids

31.82

Weight (kDa)

8.81

Isoelectric Point (pI)

55.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 174 - 274 6.1e-17 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
AatII GACGTC 1 cut(s) 309
Acc16I TGCGCA 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 231
AccII CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
AclWI GGATC 3 cut(s) 478, 535, 579
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 1 cut(s) 871
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 2 cut(s) 32, 306
AfaI GTAC 3 cut(s) 150, 284, 732
AfiI CCNNNNNNNGG 2 cut(s) 168, 704
AgsI TTSAA 1 cut(s) 560
AhdI GACNNNNNGTC 1 cut(s) 256
AjiI CACGTC 3 cut(s) 223, 436, 592
AjnI CCWGG 3 cut(s) 153, 324, 618
AluBI AGCT 7 cut(s) 292, 409, 445, 598, 662, 761, 839
AluI AGCT 7 cut(s) 292, 409, 445, 598, 662, 761, 839
Alw26I GTCTC 3 cut(s) 342, 426, 555
AlwI GGATC 3 cut(s) 478, 535, 579
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 3 cut(s) 177, 327, 609
ApeKI GCWGC 4 cut(s) 292, 409, 737, 782
ApoI RAATTY 1 cut(s) 871
Asp700I GAANNNNTTC 3 cut(s) 467, 599, 872
AspLEI GCGC 6 cut(s) 34, 43, 247, 273, 297, 349
AspS9I GGNCC 3 cut(s) 171, 178, 609
AsuC2I CCSGG 4 cut(s) 176, 276, 277, 302
AsuHPI GGTGA 1 cut(s) 391
AsuII TTCGAA 1 cut(s) 471
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 329
BanI GGYRCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 4 cut(s) 279, 396, 724, 769
BccI CCATC 3 cut(s) 338, 802, 836
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 3 cut(s) 155, 326, 620
BcnI CCSGG 4 cut(s) 176, 276, 277, 302
BcoDI GTCTC 3 cut(s) 342, 426, 555
BfaI CTAG 1 cut(s) 747
BfoI RGCGCY 2 cut(s) 35, 350
Bme1390I CCNGG 7 cut(s) 155, 176, 276, 277, 302, 326, 620
Bme18I GGWCC 1 cut(s) 171
BmeRI GACNNNNNGTC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 275
BmgBI CACGTC 3 cut(s) 223, 436, 592
BmgT120I GGNCC 3 cut(s) 171, 178, 609
BmiI GGNNCC 4 cut(s) 33, 173, 180, 610
BmrFI CCNGG 7 cut(s) 155, 176, 276, 277, 302, 326, 620
BmrI ACTGGG 1 cut(s) 664
BmuI ACTGGG 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 2 cut(s) 47, 813
Bpu14I TTCGAA 1 cut(s) 471
BpuMI CCSGG 4 cut(s) 176, 276, 277, 302
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 2 cut(s) 32, 306
BsaI GGTCTC 1 cut(s) 555
BsaJI CCNNGG 3 cut(s) 174, 274, 275
BsaXI ACNNNNNCTCC 4 cut(s) 398, 423, 428, 453
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 704
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 2 cut(s) 659, 718
BseBI CCWGG 3 cut(s) 155, 326, 620
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 3 cut(s) 174, 274, 275
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 168, 704
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 2 cut(s) 659, 718
BseXI GCAGC 4 cut(s) 279, 396, 724, 769
Bsh1236I CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
BshFI GGCC 3 cut(s) 179, 329, 611
BshNI GGYRCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 5 cut(s) 176, 276, 302, 344, 491
BsiWI CGTACG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 2 cut(s) 168, 704
BsmAI GTCTC 3 cut(s) 342, 426, 555
BsmBI CGTCTC 2 cut(s) 342, 426
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 3 cut(s) 179, 329, 611
Bso31I GGTCTC 1 cut(s) 555
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 201
Bsp1407I TGTACA 1 cut(s) 730
Bsp143I GATC 4 cut(s) 478, 483, 527, 584
Bsp68I TCGCGA 1 cut(s) 521
BspANI GGCC 3 cut(s) 179, 329, 611
BspCNI CTCAG 1 cut(s) 805
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
BspLI GGNNCC 4 cut(s) 33, 173, 180, 610
BspPI GGATC 3 cut(s) 478, 535, 579
BspQI GCTCTTC 2 cut(s) 377, 605
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 555
BsrBI CCGCTC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 343
BsrGI TGTACA 1 cut(s) 730
BsrI ACTGG 2 cut(s) 659, 718
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 174, 274, 275
BssMI GATC 4 cut(s) 478, 483, 527, 584
BssNI GRCGYC 2 cut(s) 32, 306
Bst2UI CCWGG 3 cut(s) 155, 326, 620
Bst4CI ACNGT 3 cut(s) 136, 508, 707
Bst6I CTCTTC 2 cut(s) 377, 605
BstACI GRCGYC 2 cut(s) 32, 306
BstAUI TGTACA 1 cut(s) 730
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 5 cut(s) 201, 345, 351, 453, 759
BstDEI CTNAG 2 cut(s) 47, 813
BstF5I GGATG 1 cut(s) 426
BstFNI CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
BstH2I RGCGCY 2 cut(s) 35, 350
BstHHI GCGC 6 cut(s) 34, 43, 247, 273, 297, 349
BstKTI GATC 4 cut(s) 481, 486, 530, 587
BstMAI GTCTC 3 cut(s) 342, 426, 555
BstMBI GATC 4 cut(s) 478, 483, 527, 584
BstMWI GCNNNNNNNGC 8 cut(s) 29, 38, 40, 117, 137, 277, 353, 758
BstNI CCWGG 3 cut(s) 155, 326, 620
BstSCI CCNGG 7 cut(s) 153, 174, 274, 275, 300, 324, 618
BstUI CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
BstV1I GCAGC 4 cut(s) 279, 396, 724, 769
BstX2I RGATCY 2 cut(s) 483, 527
BstXI CCANNNNNNTGG 1 cut(s) 619
BstYI RGATCY 2 cut(s) 483, 527
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 3 cut(s) 179, 329, 611
BsuTUI ATCGAT 1 cut(s) 477
BtgZI GCGATG 1 cut(s) 316
BtrI CACGTC 3 cut(s) 223, 436, 592
BtsCI GGATG 1 cut(s) 426
BtuMI TCGCGA 1 cut(s) 521
Cac8I GCNNGC 5 cut(s) 201, 345, 351, 453, 759
CfoI GCGC 6 cut(s) 34, 43, 247, 273, 297, 349
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 3 cut(s) 171, 178, 609
Cfr9I CCCGGG 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 6 cut(s) 217, 246, 251, 286, 324, 457
Csp6I GTAC 3 cut(s) 149, 283, 731
CviAII CATG 2 cut(s) 38, 67
CviQI GTAC 3 cut(s) 149, 283, 731
DdeI CTNAG 2 cut(s) 47, 813
DinI GGCGCC 1 cut(s) 33
DpnI GATC 4 cut(s) 480, 485, 529, 586
DpnII GATC 4 cut(s) 478, 483, 527, 584
DraI TTTAAA 1 cut(s) 649
DriI GACNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 377, 605
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 2 cut(s) 377, 605
EciI GGCGGA 2 cut(s) 98, 330
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 153, 324, 618
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 33
EheI GGCGCC 1 cut(s) 33
Esp3I CGTCTC 2 cut(s) 342, 426
FaeI CATG 2 cut(s) 41, 70
FaiI YATR 7 cut(s) 39, 68, 458, 606, 677, 729, 765
FaqI GGGAC 1 cut(s) 284
FatI CATG 2 cut(s) 37, 66
FauI CCCGC 6 cut(s) 104, 189, 197, 208, 410, 495
FblI GTMKAC 1 cut(s) 231
FokI GGATG 1 cut(s) 433
FriOI GRGCYC 1 cut(s) 201
FspAI RTGCGCAY 1 cut(s) 42
FspBI CTAG 1 cut(s) 747
FspI TGCGCA 1 cut(s) 42
GlaI GCGC 6 cut(s) 33, 42, 246, 272, 296, 348
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 2 cut(s) 35, 350
HaeIII GGCC 3 cut(s) 179, 329, 611
HapII CCGG 5 cut(s) 176, 276, 302, 344, 491
HgaI GACGC 6 cut(s) 217, 246, 251, 286, 324, 457
HhaI GCGC 6 cut(s) 34, 43, 247, 273, 297, 349
Hin1I GRCGYC 2 cut(s) 32, 306
Hin1II CATG 2 cut(s) 41, 70
Hin6I GCGC 6 cut(s) 32, 41, 245, 271, 295, 347
HinP1I GCGC 6 cut(s) 32, 41, 245, 271, 295, 347
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 660, 759
HinfI GANTC 3 cut(s) 209, 534, 560
HpaII CCGG 5 cut(s) 176, 276, 302, 344, 491
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 2 cut(s) 232, 673
Hpy188I TCNGA 5 cut(s) 241, 483, 504, 555, 589
Hpy188III TCNNGA 6 cut(s) 169, 310, 520, 531, 638, 747
Hpy8I GTNNAC 2 cut(s) 232, 673
Hpy99I CGWCG 7 cut(s) 140, 233, 245, 311, 508, 526, 593
HpyAV CCTTC 2 cut(s) 352, 421
HpyCH4III ACNGT 3 cut(s) 136, 508, 707
HpyCH4IV ACGT 4 cut(s) 222, 306, 435, 591
HpyCH4V TGCA 1 cut(s) 851
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 38, 40, 117, 137, 277, 353, 758
HpyF3I CTNAG 2 cut(s) 47, 813
HpySE526I ACGT 4 cut(s) 222, 306, 435, 591
Hsp92I GRCGYC 2 cut(s) 32, 306
Hsp92II CATG 2 cut(s) 41, 70
HspAI GCGC 6 cut(s) 32, 41, 245, 271, 295, 347
KasI GGCGCC 1 cut(s) 31
KroI GCCGGC 1 cut(s) 343
KroNI GCCGGC 1 cut(s) 345
Kzo9I GATC 4 cut(s) 478, 483, 527, 584
LguI GCTCTTC 2 cut(s) 377, 605
LmnI GCTCC 3 cut(s) 28, 406, 442
Lsp1109I GCAGC 4 cut(s) 279, 396, 724, 769
MaeI CTAG 1 cut(s) 747
MaeII ACGT 4 cut(s) 222, 306, 435, 591
MaeIII GTNAC 1 cut(s) 820
MalI GATC 4 cut(s) 480, 485, 529, 586
MbiI CCGCTC 1 cut(s) 184
MboI GATC 4 cut(s) 478, 483, 527, 584
MboII GAAGA 3 cut(s) 394, 436, 592
MflI RGATCY 2 cut(s) 483, 527
MhlI GDGCHC 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 3 cut(s) 496, 543, 871
MluNI TGGCCA 1 cut(s) 329
Mly113I GGCGCC 1 cut(s) 32
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 4 cut(s) 264, 527, 612, 689
Mox20I TGGCCA 1 cut(s) 329
MreI CGCCGGCG 1 cut(s) 343
MroNI GCCGGC 1 cut(s) 343
MroXI GAANNNNTTC 3 cut(s) 467, 599, 872
MscI TGGCCA 1 cut(s) 329
MseI TTAA 2 cut(s) 570, 648
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 2 cut(s) 292, 782
MspI CCGG 5 cut(s) 176, 276, 302, 344, 491
MspR9I CCNGG 7 cut(s) 155, 176, 276, 277, 302, 326, 620
MvaI CCWGG 3 cut(s) 155, 326, 620
MvnI CGCG 8 cut(s) 192, 228, 245, 257, 271, 297, 335, 521
MwoI GCNNNNNNNGC 8 cut(s) 29, 38, 40, 117, 137, 277, 353, 758
NaeI GCCGGC 1 cut(s) 345
NarI GGCGCC 1 cut(s) 32
NciI CCSGG 4 cut(s) 176, 276, 277, 302
NdeII GATC 4 cut(s) 478, 483, 527, 584
NgoMIV GCCGGC 1 cut(s) 343
NlaIII CATG 2 cut(s) 41, 70
NlaIV GGNNCC 4 cut(s) 33, 173, 180, 610
NmeAIII GCCGAG 1 cut(s) 232
NruI TCGCGA 1 cut(s) 521
NsbI TGCGCA 1 cut(s) 42
NspV TTCGAA 1 cut(s) 471
PciSI GCTCTTC 2 cut(s) 377, 605
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 228, 237
PdiI GCCGGC 1 cut(s) 345
PdmI GAANNNNTTC 3 cut(s) 467, 599, 872
PfeI GAWTC 2 cut(s) 534, 560
Pfl23II CGTACG 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 168
PfoI TCCNGGA 1 cut(s) 300
PleI GAGTC 1 cut(s) 217
PluTI GGCGCC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 217
PsiI TTATAA 1 cut(s) 765
Psp6I CCWGG 3 cut(s) 153, 324, 618
PspGI CCWGG 3 cut(s) 153, 324, 618
PspLI CGTACG 1 cut(s) 148
PspN4I GGNNCC 4 cut(s) 33, 173, 180, 610
PspPI GGNCC 3 cut(s) 171, 178, 609
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PsuI RGATCY 2 cut(s) 483, 527
PvuII CAGCTG 1 cut(s) 292
RruI TCGCGA 1 cut(s) 521
RsaI GTAC 3 cut(s) 150, 284, 732
RsaNI GTAC 3 cut(s) 149, 283, 731
SalI GTCGAC 1 cut(s) 230
SapI GCTCTTC 2 cut(s) 377, 605
SaqAI TTAA 2 cut(s) 570, 648
Sau3AI GATC 4 cut(s) 478, 483, 527, 584
Sau96I GGNCC 3 cut(s) 171, 178, 609
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 7 cut(s) 155, 176, 276, 277, 302, 326, 620
SduI GDGCHC 1 cut(s) 201
SfoI GGCGCC 1 cut(s) 33
SfuI TTCGAA 1 cut(s) 471
SgrAI CRCCGGYG 1 cut(s) 343
SinI GGWCC 1 cut(s) 171
SmaI CCCGGG 1 cut(s) 277
Sse9I AATT 3 cut(s) 496, 543, 871
SspDI GGCGCC 1 cut(s) 31
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 7 cut(s) 153, 174, 274, 275, 300, 324, 618
TaaI ACNGT 3 cut(s) 136, 508, 707
TaiI ACGT 4 cut(s) 225, 309, 438, 594
TaqI TCGA 5 cut(s) 231, 309, 398, 471, 477
TaqII GACCGA 1 cut(s) 537
TasI AATT 3 cut(s) 496, 543, 871
TatI WGTACW 1 cut(s) 730
TauI GCSGC 7 cut(s) 84, 123, 126, 143, 146, 206, 356
TfiI GAWTC 2 cut(s) 534, 560
Tru1I TTAA 2 cut(s) 570, 648
Tru9I TTAA 2 cut(s) 570, 648
TseI GCWGC 4 cut(s) 292, 409, 737, 782
TspDTI ATGAA 1 cut(s) 437
TspGWI ACGGA 1 cut(s) 15
TspMI CCCGGG 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 171
XapI RAATTY 1 cut(s) 871
XbaI TCTAGA 1 cut(s) 746
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XmaI CCCGGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 231
XmnI GAANNNNTTC 3 cut(s) 467, 599, 872
XspI CTAG 1 cut(s) 747
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.