AT1G34630

N-terminal cysteine-rich region of Transmembrane protein 135

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001014
Physical Location & Seq
Forward (+)
12685176 .. 12687641
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G34630.5

Sequence Viewer

Length: 1446 bp
ATGTCGCCGTCCGACGAATCGACGAACGGTTCTCGTTGCTCATCATGCATCTCCAGCGACAACGATGGCAACGATTTCGGTAAATCGGATGAGTTTACATGCAAACAATGCAAAAACTCCGATTCGAAATCGAAATCAAAATCGGTGAATGATTCGGATAAGCTTCGAAGAATCATCGTCGCTTCCGTCAAAGGATTCACTATAGGTACTGGTTTAAAAGGTGGTTTAGCTATTTTCTCAATCGTCGCTCGTTTCGCTCGTCGTCGTAGATCTTCTCCTCAATCTAGGAAAACCGGAGAGTTTTCGAATAGTGAAGCTATCGCTATGGGGATTAAGGAGACGTTAAGATATGGATTGTTCTTAGGAACTTTTGCTGGGACTTTTGTTTCTGTAGATGAAGCTATTGCTGCTTTAGCAGGAGACAAGAGAACTGCAAAATGGAGGGCCCTGTTTGCTGGATTGGTGGCTGGACCATCTATGCTTCTCACAGGGCCAAATACGCAACATACGAGTTTGGCTGTTTATATATTGATGCGTGCTGCGGTTCTCGCATCGCGTTGCGGTATTAAAAGTAAACGCTTTGGTACCATTTGCAAGCCACTTACTTGGAAACATGGAGATTTATTCCTCATGTGTCTCTCTTCCTCACAGATTTTGTCTGCTTACATTTTGAAACAAGAAAGCCTTCCTTCATCGTACAAGTCTTTCCTTAACAAACAAGGTGGGAAGGATCTTTCAATCTTACAAGGCGTAAAAGATATCGCAACCGCGCAACCGTTCACTAATTTAAGAGCGATTGAGAAATATTACAAGTCTGTTGGAGTTGATATCAAACTGGATCCTACCATGAAAGTCCCCTGTACGATAATACATGGAAATGAGTCATGTGTTAAGCATGGTGTTACATTTTTCCTTCAAGCTTACAAGAGGGCGTTACCTGTCTATGTCCCTGTGTATTTGATTCCAGCGCTAATAGTCCATCGTCAAGACCTGCTAAAAAAGCAATACTCTATACTTGGCAAGGGTCTTCTGGGCACAGCGAGATCAAGTTTGTTTCTCGCTACTTACTGCTCTTCTGCCTGGGCTTGGACTTGTCTACTATTCCGCACTTTTGAGACATGCAACATACCGCTTGTGGCCATAGCAACATTCCCAACAGGTCTGGCTTTAGCTATTGAGAAGAAAAGCAGAAGGATAGAGATATCACTATACTGCTTAGCGAGGGCGATAGAGAGCTTCTTTACTTGCATGACAGAGGCAGGATACATTCGACCGCCCAAGAGTTTAAGAAGAGCGGATGTGGTTGTGTTCAGCGTTTCGACAGCCATTATAATGCATTGTTACGCACAAGAAAGAGACGTATTTCGATCAAAATACTTGAACGTACTAGATTGGGTCTTCGGTGTTCCTCCTCCCCCTCCTGCTCCTTCTTGCGAAACAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich

Protein Analysis

481

Amino Acids

52.78

Weight (kDa)

9.6

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM135_C_rich PF15982 136 - 256 8.8e-08 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
Acc36I ACCTGC 1 cut(s) 999
Acc65I GGTACC 1 cut(s) 584
AccB1I GGYRCC 1 cut(s) 584
AccBSI CCGCTC 1 cut(s) 1295
AccI GTMKAC 1 cut(s) 1096
AccII CGCG 2 cut(s) 556, 770
AciI CCGC 7 cut(s) 542, 561, 768, 1105, 1130, 1274, 1295
AclWI GGATC 3 cut(s) 738, 833, 846
AcoI YGGCCR 1 cut(s) 1137
AfaI GTAC 5 cut(s) 208, 586, 698, 862, 1386
AfeI AGCGCT 1 cut(s) 969
AfiI CCNNNNNNNGG 1 cut(s) 1086
AgsI TTSAA 4 cut(s) 673, 738, 917, 1381
AjnI CCWGG 1 cut(s) 1079
AluBI AGCT 7 cut(s) 163, 230, 317, 401, 920, 1172, 1236
AluI AGCT 7 cut(s) 163, 230, 317, 401, 920, 1172, 1236
Alw26I GTCTC 5 cut(s) 332, 414, 641, 1109, 1350
AlwI GGATC 3 cut(s) 738, 833, 846
Aor51HI AGCGCT 1 cut(s) 969
AoxI GGCC 3 cut(s) 444, 491, 1137
ApaI GGGCCC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 407, 539
Asp700I GAANNNNTTC 1 cut(s) 684
Asp718I GGTACC 1 cut(s) 584
AspLEI GCGC 2 cut(s) 772, 970
AspS9I GGNCC 4 cut(s) 444, 445, 470, 491
AsuHPI GGTGA 1 cut(s) 157
AsuII TTCGAA 3 cut(s) 125, 166, 305
AvaII GGWCC 1 cut(s) 470
BaeGI GKGCMC 2 cut(s) 448, 1037
BalI TGGCCA 1 cut(s) 1139
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 448
BbsI GAAGAC 2 cut(s) 1019, 1390
BbvI GCAGC 2 cut(s) 394, 526
BccI CCATC 3 cut(s) 59, 481, 987
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 1256
BcoDI GTCTC 5 cut(s) 332, 414, 641, 1109, 1350
BfaI CTAG 3 cut(s) 285, 1388, 1444
BfmI CTRYAG 2 cut(s) 201, 390
BfoI RGCGCY 1 cut(s) 971
BfuAI ACCTGC 1 cut(s) 999
BfuI GTATCC 1 cut(s) 1256
BglII AGATCT 1 cut(s) 269
BisI GCNGC 2 cut(s) 408, 540
BlpI GCTNAGC 1 cut(s) 1216
BlsI GCNGC 2 cut(s) 409, 541
Bme1390I CCNGG 1 cut(s) 1081
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 4 cut(s) 444, 445, 470, 491
BmiI GGNNCC 3 cut(s) 446, 586, 840
BmrFI CCNGG 1 cut(s) 1081
BmsI GCATC 3 cut(s) 57, 522, 560
BpiI GAAGAC 2 cut(s) 1019, 1390
BpmI CTGGAG 1 cut(s) 37
Bpu1102I GCTNAGC 1 cut(s) 1216
Bpu14I TTCGAA 3 cut(s) 125, 166, 305
BsaJI CCNNGG 1 cut(s) 1080
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 1086
Bse1I ACTGG 2 cut(s) 214, 840
BseBI CCWGG 1 cut(s) 1081
BseDI CCNNGG 1 cut(s) 1080
BseGI GGATG 2 cut(s) 94, 1303
BseLI CCNNNNNNNGG 1 cut(s) 1086
BseNI ACTGG 2 cut(s) 214, 840
BseRI GAGGAG 2 cut(s) 267, 1401
BseSI GKGCMC 2 cut(s) 448, 1037
BseXI GCAGC 2 cut(s) 394, 526
BseYI CCCAGC 1 cut(s) 374
Bsh1236I CGCG 2 cut(s) 556, 770
Bsh1285I CGRYCG 1 cut(s) 1274
BshFI GGCC 3 cut(s) 446, 493, 1139
BshNI GGYRCC 1 cut(s) 584
BsiEI CGRYCG 1 cut(s) 1274
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 3 cut(s) 391, 839, 932
BslI CCNNNNNNNGG 1 cut(s) 1086
BsmAI GTCTC 5 cut(s) 332, 414, 641, 1109, 1350
BsmBI CGTCTC 2 cut(s) 332, 1350
BsmFI GGGAC 3 cut(s) 391, 839, 932
BsnI GGCC 3 cut(s) 446, 493, 1139
Bsp119I TTCGAA 3 cut(s) 125, 166, 305
Bsp120I GGGCCC 1 cut(s) 444
Bsp1286I GDGCHC 2 cut(s) 448, 1037
Bsp143I GATC 5 cut(s) 269, 730, 838, 1043, 1367
Bsp1720I GCTNAGC 1 cut(s) 1216
BspACI CCGC 7 cut(s) 542, 561, 768, 1105, 1130, 1274, 1295
BspANI GGCC 3 cut(s) 446, 493, 1139
BspFNI CGCG 2 cut(s) 556, 770
BspLI GGNNCC 3 cut(s) 446, 586, 840
BspMI ACCTGC 1 cut(s) 999
BspPI GGATC 3 cut(s) 738, 833, 846
BspQI GCTCTTC 2 cut(s) 1078, 1285
BspT104I TTCGAA 3 cut(s) 125, 166, 305
BspT107I GGYRCC 1 cut(s) 584
BsrBI CCGCTC 1 cut(s) 1295
BsrI ACTGG 2 cut(s) 214, 840
BssECI CCNNGG 1 cut(s) 1080
BssMI GATC 5 cut(s) 269, 730, 838, 1043, 1367
Bst2UI CCWGG 1 cut(s) 1081
Bst4CI ACNGT 2 cut(s) 29, 777
Bst6I CTCTTC 3 cut(s) 646, 1078, 1285
BstAPI GCANNNNNTGC 1 cut(s) 108
BstBI TTCGAA 3 cut(s) 125, 166, 305
BstC8I GCNNGC 2 cut(s) 537, 596
BstDEI CTNAG 2 cut(s) 361, 1216
BstF5I GGATG 2 cut(s) 94, 1303
BstFNI CGCG 2 cut(s) 556, 770
BstH2I RGCGCY 1 cut(s) 971
BstHHI GCGC 2 cut(s) 772, 970
BstKTI GATC 5 cut(s) 272, 733, 841, 1046, 1370
BstMAI GTCTC 5 cut(s) 332, 414, 641, 1109, 1350
BstMBI GATC 5 cut(s) 269, 730, 838, 1043, 1367
BstMCI CGRYCG 1 cut(s) 1274
BstNI CCWGG 1 cut(s) 1081
BstNSI RCATGY 2 cut(s) 102, 1122
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 2 cut(s) 201, 390
BstSLI GKGCMC 2 cut(s) 448, 1037
BstUI CGCG 2 cut(s) 556, 770
BstV1I GCAGC 2 cut(s) 394, 526
BstV2I GAAGAC 2 cut(s) 1019, 1390
BstX2I RGATCY 3 cut(s) 269, 730, 838
BstXI CCANNNNNNTGG 1 cut(s) 606
BstYI RGATCY 3 cut(s) 269, 730, 838
BsuI GTATCC 1 cut(s) 1256
BsuRI GGCC 3 cut(s) 446, 493, 1139
BtgZI GCGATG 1 cut(s) 537
BtsCI GGATG 2 cut(s) 94, 1303
BveI ACCTGC 1 cut(s) 999
Cac8I GCNNGC 2 cut(s) 537, 596
CfoI GCGC 2 cut(s) 772, 970
Cfr13I GGNCC 4 cut(s) 444, 445, 470, 491
Csp6I GTAC 5 cut(s) 207, 585, 697, 861, 1385
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviQI GTAC 5 cut(s) 207, 585, 697, 861, 1385
DdeI CTNAG 2 cut(s) 361, 1216
DpnI GATC 5 cut(s) 271, 732, 840, 1045, 1369
DpnII GATC 5 cut(s) 269, 730, 838, 1043, 1367
DraI TTTAAA 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 1137
Eam1104I CTCTTC 3 cut(s) 646, 1078, 1285
EarI CTCTTC 3 cut(s) 646, 1078, 1285
Eco24I GRGCYC 1 cut(s) 448
Eco32I GATATC 3 cut(s) 760, 829, 1203
Eco47I GGWCC 1 cut(s) 470
Eco47III AGCGCT 1 cut(s) 969
EcoO109I RGGNCCY 2 cut(s) 444, 445
EcoRII CCWGG 1 cut(s) 1079
EcoRV GATATC 3 cut(s) 760, 829, 1203
EcoT22I ATGCAT 2 cut(s) 50, 1338
EcoT38I GRGCYC 1 cut(s) 448
Esp3I CGTCTC 2 cut(s) 332, 1350
FalI AAGNNNNNCTT 4 cut(s) 669, 701, 673, 705
FaqI GGGAC 3 cut(s) 391, 839, 932
FblI GTMKAC 1 cut(s) 1096
Fnu4HI GCNGC 2 cut(s) 408, 540
FokI GGATG 2 cut(s) 101, 1310
FriOI GRGCYC 1 cut(s) 448
Fsp4HI GCNGC 2 cut(s) 408, 540
FspBI CTAG 3 cut(s) 285, 1388, 1444
GlaI GCGC 2 cut(s) 771, 969
GluI GCNGC 2 cut(s) 408, 540
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 37
HaeII RGCGCY 1 cut(s) 971
HaeIII GGCC 3 cut(s) 446, 493, 1139
HapII CCGG 1 cut(s) 294
HhaI GCGC 2 cut(s) 772, 970
Hin6I GCGC 2 cut(s) 770, 968
HinP1I GCGC 2 cut(s) 770, 968
HindIII AAGCTT 2 cut(s) 161, 918
HinfI GANTC 7 cut(s) 17, 122, 152, 171, 195, 881, 961
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 4 cut(s) 96, 575, 780, 1097
Hpy188I TCNGA 4 cut(s) 13, 88, 121, 157
Hpy188III TCNNGA 1 cut(s) 986
Hpy8I GTNNAC 4 cut(s) 96, 575, 780, 1097
Hpy99I CGWCG 6 cut(s) 17, 25, 182, 248, 264, 267
HpyAV CCTTC 6 cut(s) 695, 699, 721, 923, 1185, 1437
HpyCH4III ACNGT 2 cut(s) 29, 777
HpyCH4IV ACGT 3 cut(s) 341, 1359, 1383
HpyCH4V TGCA 8 cut(s) 48, 102, 111, 434, 594, 1122, 1248, 1336
HpyF3I CTNAG 2 cut(s) 361, 1216
HpySE526I ACGT 3 cut(s) 341, 1359, 1383
HspAI GCGC 2 cut(s) 770, 968
KpnI GGTACC 1 cut(s) 588
Kzo9I GATC 5 cut(s) 269, 730, 838, 1043, 1367
LguI GCTCTTC 2 cut(s) 1078, 1285
LmnI GCTCC 1 cut(s) 1429
Lsp1109I GCAGC 2 cut(s) 394, 526
LweI GCATC 3 cut(s) 57, 522, 560
MaeI CTAG 3 cut(s) 285, 1388, 1444
MaeII ACGT 3 cut(s) 341, 1359, 1383
MaeIII GTNAC 3 cut(s) 901, 933, 1340
MalI GATC 5 cut(s) 271, 732, 840, 1045, 1369
MbiI CCGCTC 1 cut(s) 1295
MboI GATC 5 cut(s) 269, 730, 838, 1043, 1367
MboII GAAGA 8 cut(s) 180, 264, 633, 1019, 1065, 1192, 1302, 1390
MflI RGATCY 3 cut(s) 269, 730, 838
MhlI GDGCHC 2 cut(s) 448, 1037
MlsI TGGCCA 1 cut(s) 1139
MluCI AATT 1 cut(s) 784
MluNI TGGCCA 1 cut(s) 1139
MlyI GAGTC 1 cut(s) 890
MmeI TCCRAC 2 cut(s) 36, 799
Mox20I TGGCCA 1 cut(s) 1139
Mph1103I ATGCAT 2 cut(s) 50, 1338
MroXI GAANNNNTTC 1 cut(s) 684
MscI TGGCCA 1 cut(s) 1139
MseI TTAA 8 cut(s) 215, 333, 344, 567, 711, 788, 891, 1286
MslI CAYNNNNRTG 2 cut(s) 876, 1331
Msp20I TGGCCA 1 cut(s) 1139
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 1081
MvaI CCWGG 1 cut(s) 1081
MvnI CGCG 2 cut(s) 556, 770
NdeII GATC 5 cut(s) 269, 730, 838, 1043, 1367
NlaIV GGNNCC 3 cut(s) 446, 586, 840
NsiI ATGCAT 2 cut(s) 50, 1338
NspI RCATGY 2 cut(s) 102, 1122
NspV TTCGAA 3 cut(s) 125, 166, 305
PciSI GCTCTTC 2 cut(s) 1078, 1285
PcsI WCGNNNNNNNCGW 4 cut(s) 69, 183, 256, 506
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 6 cut(s) 17, 122, 152, 171, 195, 961
PkrI GCNGC 2 cut(s) 409, 541
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
PsiI TTATAA 1 cut(s) 1331
Psp6I CCWGG 1 cut(s) 1079
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 1 cut(s) 1079
PspN4I GGNNCC 3 cut(s) 446, 586, 840
PspOMI GGGCCC 1 cut(s) 444
PspPI GGNCC 4 cut(s) 444, 445, 470, 491
PsuI RGATCY 3 cut(s) 269, 730, 838
RsaI GTAC 5 cut(s) 208, 586, 698, 862, 1386
RsaNI GTAC 5 cut(s) 207, 585, 697, 861, 1385
RseI CAYNNNNRTG 2 cut(s) 876, 1331
SapI GCTCTTC 2 cut(s) 1078, 1285
SaqAI TTAA 8 cut(s) 215, 333, 344, 567, 711, 788, 891, 1286
SatI GCNGC 2 cut(s) 408, 540
Sau3AI GATC 5 cut(s) 269, 730, 838, 1043, 1367
Sau96I GGNCC 4 cut(s) 444, 445, 470, 491
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 1 cut(s) 1081
SduI GDGCHC 2 cut(s) 448, 1037
SfaNI GCATC 3 cut(s) 57, 522, 560
SfcI CTRYAG 2 cut(s) 201, 390
SfuI TTCGAA 3 cut(s) 125, 166, 305
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 876, 1331
Sse9I AATT 1 cut(s) 784
SsiI CCGC 7 cut(s) 542, 561, 768, 1105, 1130, 1274, 1295
SspI AATATT 1 cut(s) 806
SspMI CTAG 3 cut(s) 285, 1388, 1444
StyD4I CCNGG 1 cut(s) 1079
TaaI ACNGT 2 cut(s) 29, 777
TaiI ACGT 3 cut(s) 344, 1362, 1386
TaqI TCGA 8 cut(s) 20, 125, 131, 166, 305, 1270, 1319, 1366
TasI AATT 1 cut(s) 784
TfiI GAWTC 6 cut(s) 17, 122, 152, 171, 195, 961
Tru1I TTAA 8 cut(s) 215, 333, 344, 567, 711, 788, 891, 1286
Tru9I TTAA 8 cut(s) 215, 333, 344, 567, 711, 788, 891, 1286
TseI GCWGC 2 cut(s) 407, 539
TspDTI ATGAA 3 cut(s) 411, 681, 863
TspGWI ACGGA 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 470
XceI RCATGY 2 cut(s) 102, 1122
XmiI GTMKAC 1 cut(s) 1096
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 3 cut(s) 285, 1388, 1444
Zsp2I ATGCAT 2 cut(s) 50, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.