Rh4AG125400

peroxisome organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001014
Physical Location & Seq
Forward (+)
27206475 .. 27207198
724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG125400.1

Sequence Viewer

Length: 471 bp
ATGTTGCCGGACGGCGATGTCTACACCTGCTGCGCCGGTGAGAATGGAGCCTCCAACGGTGATCAAGCCGAAGATCTGGTGTGCGTGCATTACAGGAAATCTGATCCCTCTCAGTCATCGTTTTGCTATTCCTCTTCGCTCAAATACGTTCCTGTGTCGTCGAGCTATTCCGAGAAGCTACGGTGGGTCGTGACTGCTTCAATTAAGGGTTTTACCATTGGCGCCGGCCTCATAGGCGGCCTTGCCGTCTTCTCCATCCTAGCTAGACTCAAAAAGAGAAAGCTCTTAGCCTCTCTCAGGACTGAGGGTGCGATTACGAATAATGAATCAATTGTTACTGTTTTGAAGGAGACCTTGAGATATGGTCTGTTTCTTGGGACTTTTGCAGGCACATTTGTTTCAGTGGATGAGATTATTGCTTCTTTGGGAGGCCACTGTAGTTCAGGACAGCTAAGTGGAGGGCTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich

Protein Analysis

156

Amino Acids

16.46

Weight (kDa)

7.57

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 35
AasI GACNNNNNNGTC 1 cut(s) 17
Acc36I ACCTGC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 21
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 222
AfiI CCNNNNNNNGG 1 cut(s) 297
AgsI TTSAA 2 cut(s) 201, 346
AluBI AGCT 5 cut(s) 165, 178, 263, 283, 451
AluI AGCT 5 cut(s) 165, 178, 263, 283, 451
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 98
AoxI GGCC 3 cut(s) 226, 238, 430
ApeKI GCWGC 1 cut(s) 30
AspLEI GCGC 2 cut(s) 35, 224
AsuHPI GGTGA 2 cut(s) 50, 71
BanI GGYRCC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 241
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 263
BceAI ACGGC 2 cut(s) 28, 230
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 2 cut(s) 260, 264
BfmI CTRYAG 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 35
BglI GCCNNNNNGGC 1 cut(s) 234
BglII AGATCT 1 cut(s) 73
BisI GCNGC 2 cut(s) 31, 238
BlsI GCNGC 2 cut(s) 32, 239
BmiI GGNNCC 2 cut(s) 49, 223
BpiI GAAGAC 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 2 cut(s) 35, 224
BseGI GGATG 2 cut(s) 255, 412
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMII CTCAG 3 cut(s) 125, 294, 310
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 3 cut(s) 228, 240, 432
BshNI GGYRCC 1 cut(s) 221
BsiSI CCGG 3 cut(s) 8, 36, 225
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 3 cut(s) 228, 240, 432
Bso31I GGTCTC 1 cut(s) 344
Bsp143I GATC 3 cut(s) 61, 73, 103
BspACI CCGC 1 cut(s) 237
BspANI GGCC 3 cut(s) 228, 240, 432
BspCNI CTCAG 3 cut(s) 124, 295, 309
BspLI GGNNCC 2 cut(s) 49, 223
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 344
BsrFI RCCGGY 2 cut(s) 35, 224
BssAI RCCGGY 2 cut(s) 35, 224
BssMI GATC 3 cut(s) 61, 73, 103
BssNI GRCGYC 1 cut(s) 222
Bst4CI ACNGT 4 cut(s) 59, 183, 340, 437
Bst6I CTCTTC 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 3 cut(s) 86, 226, 388
BstDEI CTNAG 5 cut(s) 111, 286, 296, 303, 452
BstENI CCTNNNNNAGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 255, 412
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 35, 224
BstKTI GATC 3 cut(s) 64, 76, 106
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 3 cut(s) 61, 73, 103
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 3 cut(s) 228, 240, 432
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 2 cut(s) 255, 412
BtsIMutI CAGTG 2 cut(s) 408, 433
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 3 cut(s) 86, 226, 388
CfoI GCGC 2 cut(s) 35, 224
Cfr10I RCCGGY 2 cut(s) 35, 224
DdeI CTNAG 5 cut(s) 111, 286, 296, 303, 452
DinI GGCGCC 1 cut(s) 223
DpnI GATC 3 cut(s) 63, 75, 105
DpnII GATC 3 cut(s) 61, 73, 103
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 344
EcoNI CCTNNNNNAGG 1 cut(s) 295
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaiI YATR 2 cut(s) 233, 363
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 391
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 2 cut(s) 31, 238
FokI GGATG 2 cut(s) 242, 419
Fsp4HI GCNGC 2 cut(s) 31, 238
FspBI CTAG 2 cut(s) 260, 264
GlaI GCGC 2 cut(s) 34, 223
GluI GCNGC 2 cut(s) 31, 238
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 228, 240, 432
HapII CCGG 3 cut(s) 8, 36, 225
HhaI GCGC 2 cut(s) 35, 224
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 2 cut(s) 33, 222
HinP1I GCGC 2 cut(s) 33, 222
HinfI GANTC 2 cut(s) 267, 326
HpaII CCGG 3 cut(s) 8, 36, 225
HphI GGTGA 2 cut(s) 50, 71
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 2 cut(s) 103, 172
Hpy188III TCNNGA 3 cut(s) 190, 298, 444
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 4 cut(s) 59, 183, 340, 437
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 88, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 5 cut(s) 111, 286, 296, 303, 452
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 2 cut(s) 33, 222
KasI GGCGCC 1 cut(s) 221
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 61, 73, 103
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 2 cut(s) 260, 264
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 190, 334
MalI GATC 3 cut(s) 63, 75, 105
MboI GATC 3 cut(s) 61, 73, 103
MboII GAAGA 3 cut(s) 83, 126, 241
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 73
MluCI AATT 2 cut(s) 201, 330
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 8 cut(s) 61, 118, 142, 239, 298, 301, 422, 452
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 1 cut(s) 204
MspI CCGG 3 cut(s) 8, 36, 225
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 234
NaeI GCCGGC 1 cut(s) 226
NarI GGCGCC 1 cut(s) 222
NdeII GATC 3 cut(s) 61, 73, 103
NgoMIV GCCGGC 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 49, 223
NmuCI GTSAC 1 cut(s) 190
PaqCI CACCTGC 1 cut(s) 35
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 32, 239
PleI GAGTC 1 cut(s) 261
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 49, 223
PsuI RGATCY 1 cut(s) 73
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 31, 238
Sau3AI GATC 3 cut(s) 61, 73, 103
SchI GAGTC 1 cut(s) 261
SetI ASST 8 cut(s) 29, 150, 167, 180, 265, 285, 356, 453
SfcI CTRYAG 1 cut(s) 436
SfoI GGCGCC 1 cut(s) 223
SgrAI CRCCGGYG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 2 cut(s) 201, 330
SsiI CCGC 1 cut(s) 237
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 2 cut(s) 260, 264
TaaI ACNGT 4 cut(s) 59, 183, 340, 437
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 161
TasI AATT 2 cut(s) 201, 330
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 408, 440
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 2 cut(s) 408, 440
XagI CCTNNNNNAGG 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 260, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.