RchiOBHm_Chr7g0238981

peroxisome organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001014
Physical Location & Seq
Reverse (-)
64477184 .. 64481072
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21413

Sequence Viewer

Length: 1278 bp
ATGTCGCCGGACGGCGACGTCTGCACCTGCTCCGCCGGCGATCACGCCGAAGATCCTGTGTGCGCGCATTGCAGGAAATCCGATCCCTCTCCGTCGTCGTTTTGCTATTCCTCTTCGCTCAAATACGTTCCTGTGCCGTTGAGCGATTCCGAGAAGCTACGGCGGGTCGTGACTGCTTCGATTAAGGGCTTCACCATCGGCGCCGGCCTCAAAGGCGGCCTCGCCGTCTTCTCCATCCTAGCTAGAATCAGAAAGAGAAAGCTCTTAGCCTCTCTCAGGACTGAGGGTGCGATTACGAATAATGAAGCAATTGTTACTGCTTTGAAGGAGACCTTGAGATATGGTCTGTTTCTTGGGACTTTTGCAGGCACATTTGTTTCAGTGGATGAGATTATTGCTTCTTTGGGAGGCCACCGCAGGACAGCTAAGTGGAGGGCTTTGTTAGCAGGGCTTATAGCAGGACCGTCTATGCTTCTTACCGGGCATAACACGCAGCATACGAGCTTGGCCGTATACATACTTATGCGTGCTGCCGTGTTGGCCACGCGTTGTGGGATTAAAAGCAAACGGTTTGGTCGTGTTTGTAGACCTCTAACTTGGAAGCACGGTGACATTTTCCTTATGTGTCTCTCCTCTTCGCAAATTCTGTCTGCTTACATATTGAAGCAGGAGAGTTTGCCTCTATCATATAAATCCTTTCTCAACAAACATGGGGGAAAGGATATCACAATTTTGCAAGGTGTTAAAGAGATTGCAAGTGGCTTACCGTTTACTAATTTGGAAGCAATAGAAAGATACTACAAATCTGTGGGAACAAATATTAAACTAGATCCAGCCATGAAAGTTCCCTGCACGATTGTACACGGAGGTCAGTCATGTAGCGGGCATATTATCTCTTTCCTAATTCAAGCCTACAAAAGAGCATTACCGGTTTATCTTCCAGTATATCTGATTCCAGCGCTTATAGTTCATCGGCAAGACCTCTTGAAAAGGCATTACACTATACTACTAAAGGGTCTTCTTGGCACTGCAAGATCAAGCTTGTTTCTTTCCGTATATTGCACATCTGCCTGGATGTGGACATGCTTGCTTTTTAGGATTTTCAAGATGTGTAATGTACCAATGGTAGCAATGGGGACGTTCCCGACTGGCCTGGCCTTGGGTATTGAGAAGAAAAGCAGGCGTATTGAGATATCACTCTACTGTCTCGCTCGAGCGATTGAGAGCTTTTTCACCTGCATGGCTGATACTGGATACTTGCCACAAACAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM135_C_rich

Protein Analysis

425

Amino Acids

46.6

Weight (kDa)

9.68

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM135_C_rich PF15982 292 - 412 5.1e-07 N-terminal cysteine-rich region of Transmembrane protein 135
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 35, 1244
AasI GACNNNNNNGTC 1 cut(s) 17
AatII GACGTC 1 cut(s) 21
Acc36I ACCTGC 2 cut(s) 35, 1244
AccB1I GGYRCC 1 cut(s) 200
AccI GTMKAC 2 cut(s) 513, 586
AccII CGCG 2 cut(s) 65, 547
AciI CCGC 5 cut(s) 33, 163, 216, 415, 882
AclWI GGATC 3 cut(s) 47, 77, 824
AcoI YGGCCR 2 cut(s) 507, 540
AcsI RAATTY 1 cut(s) 642
AcyI GRCGYC 2 cut(s) 18, 201
AfaI GTAC 2 cut(s) 861, 1119
AfeI AGCGCT 1 cut(s) 960
AfiI CCNNNNNNNGG 3 cut(s) 276, 1077, 1159
AflIII ACRYGT 1 cut(s) 545
AgeI ACCGGT 1 cut(s) 928
AgsI TTSAA 5 cut(s) 325, 664, 908, 988, 1105
AjnI CCWGG 2 cut(s) 1070, 1152
AluBI AGCT 7 cut(s) 157, 242, 262, 425, 504, 1041, 1227
AluI AGCT 7 cut(s) 157, 242, 262, 425, 504, 1041, 1227
Alw26I GTCTC 3 cut(s) 323, 632, 1211
AlwI GGATC 3 cut(s) 47, 77, 824
Ama87I CYCGRG 1 cut(s) 1212
Aor51HI AGCGCT 1 cut(s) 960
AoxI GGCC 7 cut(s) 205, 217, 409, 507, 540, 1150, 1155
ApeKI GCWGC 2 cut(s) 493, 530
ApoI RAATTY 1 cut(s) 642
AsiGI ACCGGT 1 cut(s) 928
AspLEI GCGC 4 cut(s) 65, 67, 203, 961
AspS9I GGNCC 1 cut(s) 461
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 184, 620, 1225
AvaI CYCGRG 1 cut(s) 1212
AvaII GGWCC 1 cut(s) 461
BalI TGGCCA 1 cut(s) 542
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 2 cut(s) 220, 1010
BbvI GCAGC 2 cut(s) 505, 517
BccI CCATC 2 cut(s) 203, 242
BceAI ACGGC 6 cut(s) 28, 121, 176, 209, 494, 518
BciT130I CCWGG 2 cut(s) 1072, 1154
BciVI GTATCC 1 cut(s) 1247
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 3 cut(s) 323, 632, 1211
BfaI CTAG 3 cut(s) 239, 243, 827
BfoI RGCGCY 2 cut(s) 204, 962
BfuAI ACCTGC 2 cut(s) 35, 1244
BfuI GTATCC 1 cut(s) 1247
BglI GCCNNNNNGGC 2 cut(s) 213, 539
BisI GCNGC 3 cut(s) 217, 494, 531
BlsI GCNGC 3 cut(s) 218, 495, 532
Bme1390I CCNGG 3 cut(s) 481, 1072, 1154
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 1 cut(s) 1212
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 3 cut(s) 481, 1072, 1154
BpiI GAAGAC 2 cut(s) 220, 1010
BplI GAGNNNNNCTC 2 cut(s) 664, 696
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 1 cut(s) 481
BsaHI GRCGYC 2 cut(s) 18, 201
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 1 cut(s) 1158
BsaWI WCCGGW 1 cut(s) 928
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 1077, 1159
Bse118I RCCGGY 3 cut(s) 35, 203, 928
Bse1I ACTGG 3 cut(s) 941, 1153, 1255
Bse3DI GCAATG 2 cut(s) 67, 1137
BseBI CCWGG 2 cut(s) 1072, 1154
BseDI CCNNGG 1 cut(s) 1158
BseGI GGATG 3 cut(s) 234, 391, 1080
BseLI CCNNNNNNNGG 3 cut(s) 276, 1077, 1159
BseMI GCAATG 2 cut(s) 67, 1137
BseMII CTCAG 2 cut(s) 273, 289
BseNI ACTGG 3 cut(s) 941, 1153, 1255
BsePI GCGCGC 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 622
BseXI GCAGC 2 cut(s) 505, 517
BsgI GTGCAG 2 cut(s) 7, 835
Bsh1236I CGCG 2 cut(s) 65, 547
BshFI GGCC 7 cut(s) 207, 219, 411, 509, 542, 1152, 1157
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 928
BsiHKCI CYCGRG 1 cut(s) 1212
BsiSI CCGG 5 cut(s) 8, 36, 204, 480, 929
BslFI GGGAC 2 cut(s) 370, 1150
BslI CCNNNNNNNGG 3 cut(s) 276, 1077, 1159
BsmAI GTCTC 3 cut(s) 323, 632, 1211
BsmFI GGGAC 2 cut(s) 370, 1150
BsnI GGCC 7 cut(s) 207, 219, 411, 509, 542, 1152, 1157
Bso31I GGTCTC 1 cut(s) 323
BsoBI CYCGRG 1 cut(s) 1212
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 5 cut(s) 40, 52, 82, 829, 1034
BspACI CCGC 5 cut(s) 33, 163, 216, 415, 882
BspANI GGCC 7 cut(s) 207, 219, 411, 509, 542, 1152, 1157
BspCNI CTCAG 2 cut(s) 274, 288
BspFNI CGCG 2 cut(s) 65, 547
BspLI GGNNCC 1 cut(s) 202
BspMI ACCTGC 2 cut(s) 35, 1244
BspPI GGATC 3 cut(s) 47, 77, 824
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 67, 1137
BsrFI RCCGGY 3 cut(s) 35, 203, 928
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 3 cut(s) 941, 1153, 1255
BssAI RCCGGY 3 cut(s) 35, 203, 928
BssECI CCNNGG 1 cut(s) 1158
BssHII GCGCGC 1 cut(s) 63
BssMI GATC 5 cut(s) 40, 52, 82, 829, 1034
BssNAI GTATAC 1 cut(s) 514
BssNI GRCGYC 2 cut(s) 18, 201
BssT1I CCWWGG 1 cut(s) 1158
Bst1107I GTATAC 1 cut(s) 514
Bst2UI CCWGG 2 cut(s) 1072, 1154
Bst4CI ACNGT 5 cut(s) 465, 570, 608, 768, 1205
Bst6I CTCTTC 2 cut(s) 118, 640
BstACI GRCGYC 2 cut(s) 18, 201
BstAUI TGTACA 1 cut(s) 859
BstC8I GCNNGC 8 cut(s) 37, 65, 205, 367, 528, 884, 1088, 1181
BstDEI CTNAG 4 cut(s) 265, 275, 282, 426
BstENI CCTNNNNNAGG 1 cut(s) 274
BstF5I GGATG 3 cut(s) 234, 391, 1080
BstFNI CGCG 2 cut(s) 65, 547
BstH2I RGCGCY 2 cut(s) 204, 962
BstHHI GCGC 4 cut(s) 65, 67, 203, 961
BstKTI GATC 5 cut(s) 43, 55, 85, 832, 1037
BstMAI GTCTC 3 cut(s) 323, 632, 1211
BstMBI GATC 5 cut(s) 40, 52, 82, 829, 1034
BstMWI GCNNNNNNNGC 7 cut(s) 21, 36, 69, 213, 443, 490, 539
BstNI CCWGG 2 cut(s) 1072, 1154
BstNSI RCATGY 1 cut(s) 1086
BstSCI CCNGG 3 cut(s) 479, 1070, 1152
BstUI CGCG 2 cut(s) 65, 547
BstV1I GCAGC 2 cut(s) 505, 517
BstV2I GAAGAC 2 cut(s) 220, 1010
BstX2I RGATCY 2 cut(s) 52, 829
BstYI RGATCY 2 cut(s) 52, 829
BstZ17I GTATAC 1 cut(s) 514
BsuI GTATCC 1 cut(s) 1247
BsuRI GGCC 7 cut(s) 207, 219, 411, 509, 542, 1152, 1157
BtsCI GGATG 3 cut(s) 234, 391, 1080
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 2 cut(s) 387, 1026
BveI ACCTGC 2 cut(s) 35, 1244
Cac8I GCNNGC 8 cut(s) 37, 65, 205, 367, 528, 884, 1088, 1181
CfoI GCGC 4 cut(s) 65, 67, 203, 961
Cfr10I RCCGGY 3 cut(s) 35, 203, 928
Cfr13I GGNCC 1 cut(s) 461
Csp6I GTAC 2 cut(s) 860, 1118
CspAI ACCGGT 1 cut(s) 928
CviAII CATG 5 cut(s) 710, 838, 876, 1083, 1240
CviQI GTAC 2 cut(s) 860, 1118
DdeI CTNAG 4 cut(s) 265, 275, 282, 426
DinI GGCGCC 1 cut(s) 202
DpnI GATC 5 cut(s) 42, 54, 84, 831, 1036
DpnII GATC 5 cut(s) 40, 52, 82, 829, 1034
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 2 cut(s) 507, 540
Eam1104I CTCTTC 2 cut(s) 118, 640
EarI CTCTTC 2 cut(s) 118, 640
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 1158
Eco31I GGTCTC 1 cut(s) 323
Eco32I GATATC 2 cut(s) 724, 1194
Eco47I GGWCC 1 cut(s) 461
Eco47III AGCGCT 1 cut(s) 960
Eco88I CYCGRG 1 cut(s) 1212
EcoNI CCTNNNNNAGG 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 1070, 1152
EcoRV GATATC 2 cut(s) 724, 1194
EcoT14I CCWWGG 1 cut(s) 1158
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 1158
FaeI CATG 5 cut(s) 713, 841, 879, 1086, 1243
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 2 cut(s) 370, 1150
FatI CATG 5 cut(s) 709, 837, 875, 1082, 1239
FauI CCCGC 2 cut(s) 156, 875
FblI GTMKAC 2 cut(s) 513, 586
Fnu4HI GCNGC 3 cut(s) 217, 494, 531
FokI GGATG 3 cut(s) 221, 398, 1087
Fsp4HI GCNGC 3 cut(s) 217, 494, 531
FspBI CTAG 3 cut(s) 239, 243, 827
GlaI GCGC 4 cut(s) 64, 66, 202, 960
GluI GCNGC 3 cut(s) 217, 494, 531
HaeII RGCGCY 2 cut(s) 204, 962
HaeIII GGCC 7 cut(s) 207, 219, 411, 509, 542, 1152, 1157
HapII CCGG 5 cut(s) 8, 36, 204, 480, 929
HhaI GCGC 4 cut(s) 65, 67, 203, 961
Hin1I GRCGYC 2 cut(s) 18, 201
Hin1II CATG 5 cut(s) 713, 841, 879, 1086, 1243
Hin6I GCGC 4 cut(s) 63, 65, 201, 959
HinP1I GCGC 4 cut(s) 63, 65, 201, 959
HindIII AAGCTT 1 cut(s) 1039
HinfI GANTC 3 cut(s) 146, 246, 952
HpaII CCGG 5 cut(s) 8, 36, 204, 480, 929
HphI GGTGA 3 cut(s) 184, 620, 1225
Hpy166II GTNNAC 5 cut(s) 514, 587, 771, 862, 1080
Hpy188I TCNGA 4 cut(s) 82, 151, 251, 951
Hpy188III TCNNGA 5 cut(s) 169, 277, 985, 1105, 1144
Hpy8I GTNNAC 5 cut(s) 514, 587, 771, 862, 1080
Hpy99I CGWCG 3 cut(s) 20, 97, 100
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 5 cut(s) 465, 570, 608, 768, 1205
HpyCH4IV ACGT 3 cut(s) 18, 126, 1139
HpyCH4V TGCA 9 cut(s) 24, 72, 365, 736, 755, 852, 1031, 1062, 1239
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 36, 69, 213, 443, 490, 539
HpyF3I CTNAG 4 cut(s) 265, 275, 282, 426
HpySE526I ACGT 3 cut(s) 18, 126, 1139
Hsp92I GRCGYC 2 cut(s) 18, 201
Hsp92II CATG 5 cut(s) 713, 841, 879, 1086, 1243
HspAI GCGC 4 cut(s) 63, 65, 201, 959
KasI GGCGCC 1 cut(s) 200
KroI GCCGGC 2 cut(s) 35, 203
KroNI GCCGGC 2 cut(s) 37, 205
Kzo9I GATC 5 cut(s) 40, 52, 82, 829, 1034
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 2 cut(s) 505, 517
MaeI CTAG 3 cut(s) 239, 243, 827
MaeII ACGT 3 cut(s) 18, 126, 1139
MaeIII GTNAC 3 cut(s) 169, 313, 608
MalI GATC 5 cut(s) 42, 54, 84, 831, 1036
MboI GATC 5 cut(s) 40, 52, 82, 829, 1034
MboII GAAGA 7 cut(s) 62, 105, 220, 627, 929, 1010, 1183
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 2 cut(s) 52, 829
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 6 cut(s) 309, 642, 729, 775, 903, 1273
MluI ACGCGT 1 cut(s) 545
MluNI TGGCCA 1 cut(s) 542
Mly113I GGCGCC 1 cut(s) 201
Mox20I TGGCCA 1 cut(s) 542
MreI CGCCGGCG 1 cut(s) 35
MroNI GCCGGC 2 cut(s) 35, 203
MscI TGGCCA 1 cut(s) 542
MseI TTAA 4 cut(s) 183, 558, 744, 822
MslI CAYNNNNRTG 2 cut(s) 521, 1238
Msp20I TGGCCA 1 cut(s) 542
MspI CCGG 5 cut(s) 8, 36, 204, 480, 929
MspR9I CCNGG 3 cut(s) 481, 1072, 1154
MunI CAATTG 1 cut(s) 309
MvaI CCWGG 2 cut(s) 1072, 1154
MvnI CGCG 2 cut(s) 65, 547
MwoI GCNNNNNNNGC 7 cut(s) 21, 36, 69, 213, 443, 490, 539
NaeI GCCGGC 2 cut(s) 37, 205
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 1 cut(s) 481
NdeII GATC 5 cut(s) 40, 52, 82, 829, 1034
NgoMIV GCCGGC 2 cut(s) 35, 203
NlaIII CATG 5 cut(s) 713, 841, 879, 1086, 1243
NlaIV GGNNCC 1 cut(s) 202
NmuCI GTSAC 2 cut(s) 169, 608
NspI RCATGY 1 cut(s) 1086
PaeR7I CTCGAG 1 cut(s) 1212
PaqCI CACCTGC 2 cut(s) 35, 1244
PauI GCGCGC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 2 cut(s) 497, 574
PdiI GCCGGC 2 cut(s) 37, 205
PfeI GAWTC 3 cut(s) 146, 246, 952
PinAI ACCGGT 1 cut(s) 928
PkrI GCNGC 3 cut(s) 218, 495, 532
PluTI GGCGCC 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 1070, 1152
PspGI CCWGG 2 cut(s) 1070, 1152
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 1212
PsuI RGATCY 2 cut(s) 52, 829
PteI GCGCGC 1 cut(s) 63
RsaI GTAC 2 cut(s) 861, 1119
RsaNI GTAC 2 cut(s) 860, 1118
RseI CAYNNNNRTG 2 cut(s) 521, 1238
SaqAI TTAA 4 cut(s) 183, 558, 744, 822
SatI GCNGC 3 cut(s) 217, 494, 531
Sau3AI GATC 5 cut(s) 40, 52, 82, 829, 1034
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 481, 1072, 1154
SfoI GGCGCC 1 cut(s) 202
Sfr274I CTCGAG 1 cut(s) 1212
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 461
SlaI CTCGAG 1 cut(s) 1212
SmiMI CAYNNNNRTG 2 cut(s) 521, 1238
SmlI CTYRAG 2 cut(s) 334, 1212
SmoI CTYRAG 2 cut(s) 334, 1212
Sse9I AATT 6 cut(s) 309, 642, 729, 775, 903, 1273
SsiI CCGC 5 cut(s) 33, 163, 216, 415, 882
SspDI GGCGCC 1 cut(s) 200
SspI AATATT 1 cut(s) 820
SspMI CTAG 3 cut(s) 239, 243, 827
StyD4I CCNGG 3 cut(s) 479, 1070, 1152
StyI CCWWGG 1 cut(s) 1158
TaaI ACNGT 5 cut(s) 465, 570, 608, 768, 1205
TaiI ACGT 3 cut(s) 21, 129, 1142
TaqI TCGA 2 cut(s) 179, 1213
TasI AATT 6 cut(s) 309, 642, 729, 775, 903, 1273
TatI WGTACW 1 cut(s) 859
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 3 cut(s) 146, 246, 952
Tru1I TTAA 4 cut(s) 183, 558, 744, 822
Tru9I TTAA 4 cut(s) 183, 558, 744, 822
TscAI CASTG 2 cut(s) 387, 1033
TseFI GTSAC 2 cut(s) 169, 608
TseI GCWGC 2 cut(s) 493, 530
Tsp45I GTSAC 2 cut(s) 169, 608
TspDTI ATGAA 3 cut(s) 318, 854, 959
TspGWI ACGGA 3 cut(s) 81, 879, 1042
TspRI CASTG 2 cut(s) 387, 1033
VpaK11BI GGWCC 1 cut(s) 461
XagI CCTNNNNNAGG 1 cut(s) 274
XapI RAATTY 1 cut(s) 642
XceI RCATGY 1 cut(s) 1086
XhoI CTCGAG 1 cut(s) 1212
XmiI GTMKAC 2 cut(s) 513, 586
XspI CTAG 3 cut(s) 239, 243, 827
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.