AT2G33150

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001874
Physical Location & Seq
Reverse (-)
14047478 .. 14051400
3923 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33150.1

Sequence Viewer

Length: 1389 bp
ATGGAGAAAGCGATCGAGAGACAACGCGTTCTTCTTGAGCATCTCCGACCTTCTTCTTCTTCTTCGCACAATTACGAGGCTTCTCTATCTGCTTCTGCTTGCTTGGCTGGGGACAGTGCTGCATATCAGAGGACCTCTCTCTATGGAGATGATGTTGTCATTGTCGCGGCACATAGGACTCCACTATGCAAGTCCAAACGTGGCAATTTCAAGGATACATATCCCGATGATTTGCTCGCACCTGTTTTGAGGGCATTGATAGAGAAGACGAATCTAAACCCAAGTGAAGTAGGTGACATTGTTGTGGGTACTGTTTTGGCACCTGGATCTCAGAGAGCCAGTGAATGCAGGATGGCTGCGTTCTATGCTGGTTTCCCTGAAACCGTGGCTGTCAGAACTGTGAATAGACAGTGCTCATCTGGGCTTCAGGCTGTTGCTGATGTAGCCGCTGCCATTAAAGCGGGATTTTATGACATTGGTATCGGGGCTGGTTTGGAGTCCATGACTACCAATCCAATGGCATGGGAAGGGTCAGTCAACCCAGCGGTGAAGAAGTTTGCACAAGCGCAGAATTGTCTTCTTCCTATGGGTGTTACGTCAGAAAATGTAGCACAACGCTTTGGTGTCTCAAGGCAGGAGCAAGATCAAGCTGCTGTTGACTCGCACAGAAAGGCAGCTGCTGCTACTGCTGCTGGTAAATTCAAGGATGAGATCATTCCTGTTAAGACCAAGCTTGTTGACCCGAAGACTGGTGATGAGAAACCCATTACAGTTTCTGTTGATGATGGTATCCGACCAACCACAACTCTTGCTTCTCTTGGGAAGCTGAAGCCAGTGTTTAAGAAGGATGGCACCACTACTGCTGGAAATTCCAGCCAAGTAAGTGATGGTGCAGGAGCGGTTCTCCTAATGAAGAGAAGTGTTGCAATGCAAAAAGGACTTCCCGTTCTTGGTGTATTCAGGACATTTGCTGCAGTTGGTGTTGACCCTGCAATCATGGGTATCGGTCCAGCAGTTGCCATTCCTGCTGCAGTTAAGGCGGCTGGTTTAGAACTTGATGACATCGACTTGTTTGAGATCAATGAGGCATTTGCATCTCAGTTTGTTTATTGCCGTAACAAATTGGGACTTGACCCAGAGAAAATCAATGTCAACGGAGGTGCAATGGCCATAGGCCATCCTTTGGGCGCTACAGGAGCGCGTTGTGTTGCTACATTGTTGCACGAGATGAAACGCCGTGGTAAAGACTGCCGTTTTGGAGTAGTGTCAATGTGCATTGGGACGGGGATGGGTGCAGCAGCTGTGTTTGAGAGAGGAGATGGAGTTGATGAGCTTCGCAACGCAAGGAAAGTTGAAGCGCAAGGTCTTTTGTCCAAGGACGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

462

Amino Acids

48.58

Weight (kDa)

8.62

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 52 - 307 3e-78 Thiolase, N-terminal domain
Thiolase_C PF02803 316 - 438 1.2e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 319, 851
AccB7I CCANNNNNTGG 1 cut(s) 1183
AccBSI CCGCTC 1 cut(s) 899
AccII CGCG 3 cut(s) 27, 167, 1201
AciI CCGC 6 cut(s) 167, 447, 461, 545, 899, 1040
AclWI GGATC 1 cut(s) 334
AcoI YGGCCR 1 cut(s) 1167
AcsI RAATTY 2 cut(s) 698, 868
AcuI CTGAAG 2 cut(s) 410, 848
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 3 cut(s) 749, 950, 1183
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 3 cut(s) 211, 703, 1355
AjnI CCWGG 1 cut(s) 322
AloI GAACNNNNNNTCC 2 cut(s) 930, 962
AluBI AGCT 6 cut(s) 650, 677, 733, 826, 1301, 1333
AluI AGCT 6 cut(s) 650, 677, 733, 826, 1301, 1333
Alw21I GWGCWC 1 cut(s) 416
Alw26I GTCTC 2 cut(s) 13, 631
AlwI GGATC 1 cut(s) 334
AlwNI CAGNNNCTG 3 cut(s) 680, 776, 1301
AoxI GGCC 2 cut(s) 1167, 1174
ApoI RAATTY 2 cut(s) 698, 868
ArsI GACNNNNNNTTYG 2 cut(s) 1238, 1270
AspLEI GCGC 4 cut(s) 568, 1190, 1201, 1360
AspS9I GGNCC 2 cut(s) 132, 1007
AsuHPI GGTGA 3 cut(s) 305, 559, 764
AvaII GGWCC 2 cut(s) 132, 1007
BaeI ACNNNNGTAYC 2 cut(s) 463, 496
BalI TGGCCA 1 cut(s) 1169
BanI GGYRCC 2 cut(s) 319, 851
BauI CACGAG 1 cut(s) 1223
BbsI GAAGAC 3 cut(s) 272, 569, 752
Bbv12I GWGCWC 1 cut(s) 416
BccI CCATC 7 cut(s) 346, 779, 842, 881, 1185, 1282, 1313
BceAI ACGGC 3 cut(s) 1098, 1221, 1236
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 2 cut(s) 208, 800
BcoDI GTCTC 2 cut(s) 13, 631
BfaI CTAG 1 cut(s) 1387
BfmI CTRYAG 3 cut(s) 972, 1029, 1191
BfoI RGCGCY 1 cut(s) 1191
BfuI GTATCC 2 cut(s) 208, 800
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 2 cut(s) 132, 1007
BmgT120I GGNCC 2 cut(s) 132, 1007
BmiI GGNNCC 2 cut(s) 321, 853
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 49, 1103
BpiI GAAGAC 3 cut(s) 272, 569, 752
BplI GAGNNNNNCTC 4 cut(s) 121, 153, 888, 920
BpuEI CTTGAG 2 cut(s) 56, 613
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 384, 1237, 1374
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 1308, 1338
Bsc4I CCNNNNNNNGG 3 cut(s) 749, 950, 1183
Bse1I ACTGG 3 cut(s) 339, 754, 833
Bse3DI GCAATG 2 cut(s) 933, 1170
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 3 cut(s) 384, 1237, 1374
BseGI GGATG 5 cut(s) 357, 712, 853, 1177, 1293
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 3 cut(s) 749, 950, 1183
BseMI GCAATG 2 cut(s) 933, 1170
BseMII CTCAG 2 cut(s) 344, 1112
BseNI ACTGG 3 cut(s) 339, 754, 833
BseRI GAGGAG 1 cut(s) 1329
BseYI CCCAGC 2 cut(s) 107, 541
BsgI GTGCAG 2 cut(s) 912, 1314
Bsh1236I CGCG 3 cut(s) 27, 167, 1201
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 2 cut(s) 1169, 1176
BshNI GGYRCC 2 cut(s) 319, 851
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 416
BslFI GGGAC 3 cut(s) 125, 1140, 1294
BslI CCNNNNNNNGG 3 cut(s) 749, 950, 1183
BsmAI GTCTC 2 cut(s) 13, 631
BsmFI GGGAC 3 cut(s) 125, 1140, 1294
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 2 cut(s) 1169, 1176
Bsp1286I GDGCHC 1 cut(s) 416
Bsp143I GATC 5 cut(s) 12, 326, 643, 711, 1077
BspACI CCGC 6 cut(s) 167, 447, 461, 545, 899, 1040
BspANI GGCC 2 cut(s) 1169, 1176
BspCNI CTCAG 2 cut(s) 343, 1111
BspFNI CGCG 3 cut(s) 27, 167, 1201
BspLI GGNNCC 2 cut(s) 321, 853
BspMAI CTGCAG 2 cut(s) 976, 1033
BspPI GGATC 1 cut(s) 334
BspT107I GGYRCC 2 cut(s) 319, 851
BsrBI CCGCTC 1 cut(s) 899
BsrDI GCAATG 2 cut(s) 933, 1170
BsrI ACTGG 3 cut(s) 339, 754, 833
BssECI CCNNGG 3 cut(s) 384, 1237, 1374
BssMI GATC 5 cut(s) 12, 326, 643, 711, 1077
BssSI CACGAG 1 cut(s) 1223
BssT1I CCWWGG 1 cut(s) 1374
Bst2BI CACGAG 1 cut(s) 1223
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 6 cut(s) 116, 313, 385, 400, 411, 772
Bst6I CTCTTC 1 cut(s) 908
BstAPI GCANNNNNTGC 1 cut(s) 680
BstC8I GCNNGC 3 cut(s) 100, 237, 1384
BstDEI CTNAG 2 cut(s) 330, 1098
BstDSI CCRYGG 2 cut(s) 384, 1237
BstF5I GGATG 5 cut(s) 357, 712, 853, 1177, 1293
BstFNI CGCG 3 cut(s) 27, 167, 1201
BstH2I RGCGCY 1 cut(s) 1191
BstHHI GCGC 4 cut(s) 568, 1190, 1201, 1360
BstKTI GATC 5 cut(s) 15, 329, 646, 714, 1080
BstMAI GTCTC 2 cut(s) 13, 631
BstMBI GATC 5 cut(s) 12, 326, 643, 711, 1077
BstMCI CGRYCG 1 cut(s) 15
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 3 cut(s) 972, 1029, 1191
BstUI CGCG 3 cut(s) 27, 167, 1201
BstV2I GAAGAC 3 cut(s) 272, 569, 752
BstX2I RGATCY 1 cut(s) 326
BstXI CCANNNNNNTGG 2 cut(s) 517, 522
BstYI RGATCY 1 cut(s) 326
BsuI GTATCC 2 cut(s) 208, 800
BsuRI GGCC 2 cut(s) 1169, 1176
BtgI CCRYGG 2 cut(s) 384, 1237
BtsCI GGATG 5 cut(s) 357, 712, 853, 1177, 1293
BtsIMutI CAGTG 4 cut(s) 121, 346, 416, 840
Cac8I GCNNGC 3 cut(s) 100, 237, 1384
CaiI CAGNNNCTG 3 cut(s) 680, 776, 1301
CfoI GCGC 4 cut(s) 568, 1190, 1201, 1360
Cfr13I GGNCC 2 cut(s) 132, 1007
Csp6I GTAC 1 cut(s) 309
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 3 cut(s) 502, 522, 997
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 2 cut(s) 330, 1098
DpnI GATC 5 cut(s) 14, 328, 645, 713, 1079
DpnII GATC 5 cut(s) 12, 326, 643, 711, 1077
EaeI YGGCCR 1 cut(s) 1167
Eam1104I CTCTTC 1 cut(s) 908
EarI CTCTTC 1 cut(s) 908
Eco130I CCWWGG 1 cut(s) 1374
Eco47I GGWCC 2 cut(s) 132, 1007
Eco57I CTGAAG 2 cut(s) 410, 848
EcoO109I RGGNCCY 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 1374
ErhI CCWWGG 1 cut(s) 1374
FaeI CATG 3 cut(s) 505, 525, 1000
FaqI GGGAC 3 cut(s) 125, 1140, 1294
FatI CATG 3 cut(s) 501, 521, 996
FauI CCCGC 1 cut(s) 454
FokI GGATG 5 cut(s) 364, 719, 860, 1164, 1300
FspBI CTAG 1 cut(s) 1387
GlaI GCGC 4 cut(s) 567, 1189, 1200, 1359
GsaI CCCAGC 2 cut(s) 111, 545
HaeII RGCGCY 1 cut(s) 1191
HaeIII GGCC 2 cut(s) 1169, 1176
HhaI GCGC 4 cut(s) 568, 1190, 1201, 1360
Hin1II CATG 3 cut(s) 505, 525, 1000
Hin6I GCGC 4 cut(s) 566, 1188, 1199, 1358
HinP1I GCGC 4 cut(s) 566, 1188, 1199, 1358
HincII GTYRAC 5 cut(s) 538, 658, 739, 985, 1153
HindII GTYRAC 5 cut(s) 538, 658, 739, 985, 1153
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 4 cut(s) 178, 271, 497, 659
HphI GGTGA 3 cut(s) 305, 559, 764
Hpy166II GTNNAC 5 cut(s) 538, 658, 739, 985, 1153
Hpy188I TCNGA 6 cut(s) 47, 129, 333, 395, 601, 794
Hpy188III TCNNGA 4 cut(s) 16, 35, 224, 961
Hpy8I GTNNAC 5 cut(s) 538, 658, 739, 985, 1153
HpyAV CCTTC 3 cut(s) 60, 521, 838
HpyCH4III ACNGT 6 cut(s) 116, 313, 385, 400, 411, 772
HpyCH4IV ACGT 2 cut(s) 199, 596
HpyF3I CTNAG 2 cut(s) 330, 1098
HpySE526I ACGT 2 cut(s) 199, 596
Hsp92II CATG 3 cut(s) 505, 525, 1000
HspAI GCGC 4 cut(s) 566, 1188, 1199, 1358
Kzo9I GATC 5 cut(s) 12, 326, 643, 711, 1077
LmnI GCTCC 3 cut(s) 637, 896, 1196
LweI GCATC 2 cut(s) 49, 1103
MaeI CTAG 1 cut(s) 1387
MaeII ACGT 2 cut(s) 199, 596
MaeIII GTNAC 3 cut(s) 293, 592, 1115
MalI GATC 5 cut(s) 14, 328, 645, 713, 1079
MbiI CCGCTC 1 cut(s) 899
MboI GATC 5 cut(s) 12, 326, 643, 711, 1077
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 1 cut(s) 416
MlsI TGGCCA 1 cut(s) 1169
MluCI AATT 6 cut(s) 70, 205, 571, 698, 868, 1121
MluI ACGCGT 1 cut(s) 25
MluNI TGGCCA 1 cut(s) 1169
MlyI GAGTC 3 cut(s) 172, 506, 653
MmeI TCCRAC 2 cut(s) 70, 817
MnlI CCTC 7 cut(s) 70, 123, 145, 243, 1078, 1151, 1307
Mox20I TGGCCA 1 cut(s) 1169
MscI TGGCCA 1 cut(s) 1169
MseI TTAA 4 cut(s) 456, 723, 840, 1035
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 1169
MspA1I CMGCKG 4 cut(s) 449, 545, 677, 1301
MspR9I CCNGG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 350
MvaI CCWGG 1 cut(s) 324
MvnI CGCG 3 cut(s) 27, 167, 1201
NdeII GATC 5 cut(s) 12, 326, 643, 711, 1077
NlaIII CATG 3 cut(s) 505, 525, 1000
NlaIV GGNNCC 2 cut(s) 321, 853
NmuCI GTSAC 1 cut(s) 293
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 1183
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 3 cut(s) 172, 505, 653
PpsI GAGTC 3 cut(s) 172, 505, 653
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 2 cut(s) 107, 541
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 321, 853
PspPI GGNCC 2 cut(s) 132, 1007
PspPPI RGGWCCY 1 cut(s) 132
PstI CTGCAG 2 cut(s) 976, 1033
PstNI CAGNNNCTG 3 cut(s) 680, 776, 1301
PsuI RGATCY 1 cut(s) 326
PvuI CGATCG 1 cut(s) 15
PvuII CAGCTG 2 cut(s) 677, 1301
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 4 cut(s) 456, 723, 840, 1035
Sau3AI GATC 5 cut(s) 12, 326, 643, 711, 1077
Sau96I GGNCC 2 cut(s) 132, 1007
SchI GAGTC 3 cut(s) 172, 506, 653
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 416
SfaNI GCATC 2 cut(s) 49, 1103
SfcI CTRYAG 3 cut(s) 972, 1029, 1191
SinI GGWCC 2 cut(s) 132, 1007
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 2 cut(s) 35, 628
SmoI CTYRAG 2 cut(s) 35, 628
Sse9I AATT 6 cut(s) 70, 205, 571, 698, 868, 1121
SsiI CCGC 6 cut(s) 167, 447, 461, 545, 899, 1040
SspMI CTAG 1 cut(s) 1387
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 1374
TaaI ACNGT 6 cut(s) 116, 313, 385, 400, 411, 772
TaiI ACGT 2 cut(s) 202, 599
TaqI TCGA 2 cut(s) 15, 1065
TaqII GACCGA 1 cut(s) 995
TasI AATT 6 cut(s) 70, 205, 571, 698, 868, 1121
TauI GCSGC 3 cut(s) 170, 449, 1043
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 4 cut(s) 456, 723, 840, 1035
Tru9I TTAA 4 cut(s) 456, 723, 840, 1035
TscAI CASTG 4 cut(s) 121, 346, 416, 840
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 926, 1244
TspGWI ACGGA 1 cut(s) 1170
TspRI CASTG 4 cut(s) 121, 346, 416, 840
Van91I CCANNNNNTGG 1 cut(s) 1183
VpaK11BI GGWCC 2 cut(s) 132, 1007
XapI RAATTY 2 cut(s) 698, 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 884
XspI CTAG 1 cut(s) 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.