Rw4G000620

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001874
Physical Location & Seq
Forward (+)
1237012 .. 1240467
3456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000620.1

Sequence Viewer

Length: 1392 bp
ATGGAGAAAGCGATCAACAGGCAGAAGGTTCTCCTTGACCACCTCCGACCTTCTTCTTCTTCTTCTTCTTCTTCTACCGACGACTCTTCTCTCTCCGCGTCGGCATGTGCCGCTGGGGATAGTGCTGCCTATGCAAGGACAAACGTGTTTGGGGATGATGTCGTTATTGTAGCAGCTTATCGCACTCCACTCTGCAAGGCCAAACGCGGCGGCTTCAAAGATACTCATGCCGATGATCTTCTTGCGCCTGTTCTCAAGGCAGTGATTGAGAAAACCAATCTGAATCCGAAGGAAGTTGGGGATATTGTTGTCGGTACCGTGTTGGCTCCCGGATCTCAAAGAGCTAGCGAATGCAGGATGGCTGCTTTCTATGCTGGCTTCCCTGAAACTGTGCCGGTTAGAACTGTGAACAGACAATGTTCGTCTGGGCTTCAAGCAGTTGCTGATGTAGCTGCTTCTATAAGAGCAGGGTTTTATGACATTGGTATTGGAGCCGGGTTGGAATCCATGACTGTAAACCCAATGGCATGGGAAGGGGATGTTAATCCTAAAGTAAAGATCTTTGAACAAGCCCAGAATTGCCTTCTTCCTATGGGGGTCACCTCGGAAAATGTTGCTCATCGTTTTGGTGTTTCAAGGCAGGAGCAAGATCAGGCTGCAGTTGACTCTCATAGAAAGGCAGCTGCTGCTACTGCTGCTGGTAGATTTAAAGATGAAATTATCCCTGTGGCAACCAAGATTGTTGATCCAAAATCTGGTGATGAGAAACCTGTTACAATCTCTGTTGATGATGGGATTCGAAACACAACATTGTCGGACCTAGCAAAGCTGAAGCCTGTGTTTAAGAAAGATGGGACCACCACTGCTGGTAATTCTAGTCAAGTTAGTGATGGTGCTGGAGCTGTTCTCTTGATGAAGAGAAGTGTTGCCGACCAAAAAGGAATTCCAATTCTTGGTGTATTCAGGAGTTTTGTTGCTGTTGGTGTGGATCCTGCCATCATGGGTGTTGGCCCAGCTGCTGCAATTCCAGTTGCAGTCAAGGCAGCTGGTTTAGAGCTTGATGATATTGACCTTTTTGAGATAAATGAGGCTTTTGCTTCCCAATTTGTGTATTGCCGTAACAAGCTAGGACTTGATCCAGAAAAAATCAATGTTAATGGAGGTGCATTGGCCATTGGCCATCCACTTGGTGCAACAGGTGCCCGTTGTGTTGCCACTCTTTTGCACGAGATGAAGCGTCGGGGAAAAGACTGTCGCTATGGAGTGATCTCAATGTGCATAGGCACAGGGATGGGTGCAGCCGCTGTTTTTGAAAGGGGGGACTGCACTGATGAACTCTGTAATGCTCGCAAGGTTGAATCCCTCAATTTTTTATCCAAGGATGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

463

Amino Acids

48.62

Weight (kDa)

6.99

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 54 - 308 9.2e-78 Thiolase, N-terminal domain
Thiolase_C PF02803 317 - 439 2.6e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 2 cut(s) 314, 1199
AccB7I CCANNNNNTGG 2 cut(s) 755, 953
AccII CGCG 2 cut(s) 98, 207
AciI CCGC 5 cut(s) 96, 111, 207, 210, 1302
AclWI GGATC 5 cut(s) 340, 740, 983, 996, 1130
AcoI YGGCCR 2 cut(s) 1170, 1177
AcsI RAATTY 1 cut(s) 942
AcuI CTGAAG 1 cut(s) 851
AdeI CACNNNGTG 1 cut(s) 1190
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 3 cut(s) 135, 755, 953
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 6 cut(s) 217, 434, 566, 636, 1313, 1358
AjuI GAANNNNNNNTTGG 2 cut(s) 927, 959
AlwI GGATC 5 cut(s) 340, 740, 983, 996, 1130
AlwNI CAGNNNCTG 4 cut(s) 443, 686, 1019, 1304
AoxI GGCC 4 cut(s) 198, 1009, 1170, 1177
ApoI RAATTY 1 cut(s) 942
Asp718I GGTACC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 817, 855, 1010
AsuC2I CCSGG 2 cut(s) 330, 496
AsuHPI GGTGA 2 cut(s) 592, 770
AsuII TTCGAA 1 cut(s) 799
AsuNHI GCTAGC 1 cut(s) 344
AvaII GGWCC 2 cut(s) 817, 855
BaeGI GKGCMC 1 cut(s) 1204
BalI TGGCCA 2 cut(s) 1172, 1179
BamHI GGATCC 1 cut(s) 988
BanI GGYRCC 2 cut(s) 314, 1199
BauI CACGAG 1 cut(s) 1226
BccI CCATC 7 cut(s) 352, 785, 845, 884, 1004, 1188, 1285
BceAI ACGGC 1 cut(s) 1101
BcnI CCSGG 2 cut(s) 330, 496
BfaI CTAG 4 cut(s) 345, 821, 876, 1127
BfmI CTRYAG 1 cut(s) 657
BglII AGATCT 1 cut(s) 558
Bme1390I CCNGG 2 cut(s) 330, 496
Bme18I GGWCC 2 cut(s) 817, 855
BmgT120I GGNCC 3 cut(s) 817, 855, 1010
BmiI GGNNCC 6 cut(s) 316, 327, 493, 856, 990, 1201
BmrFI CCNGG 2 cut(s) 330, 496
BmsI GCATC 1 cut(s) 1372
BmtI GCTAGC 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpmI CTGGAG 1 cut(s) 918
Bpu14I TTCGAA 1 cut(s) 799
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 330, 496
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 603, 1377
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 755, 953
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 1028
Bse8I GATNNNNATC 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 603, 1377
BseGI GGATG 6 cut(s) 160, 363, 544, 1180, 1296, 1387
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 135, 755, 953
BseNI ACTGG 1 cut(s) 1028
BseSI GKGCMC 1 cut(s) 1204
BseYI CCCAGC 2 cut(s) 113, 1012
BsgI GTGCAG 2 cut(s) 1309, 1317
Bsh1236I CGCG 2 cut(s) 98, 207
BshFI GGCC 4 cut(s) 200, 1011, 1172, 1179
BshNI GGYRCC 2 cut(s) 314, 1199
BsiSI CCGG 3 cut(s) 330, 395, 495
BslFI GGGAC 2 cut(s) 868, 1334
BslI CCNNNNNNNGG 3 cut(s) 135, 755, 953
BsmFI GGGAC 2 cut(s) 868, 1334
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 4 cut(s) 200, 1011, 1172, 1179
Bsp119I TTCGAA 1 cut(s) 799
Bsp1286I GDGCHC 1 cut(s) 1204
Bsp143I GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
BspACI CCGC 5 cut(s) 96, 111, 207, 210, 1302
BspANI GGCC 4 cut(s) 200, 1011, 1172, 1179
BspFNI CGCG 2 cut(s) 98, 207
BspLI GGNNCC 6 cut(s) 316, 327, 493, 856, 990, 1201
BspMAI CTGCAG 1 cut(s) 661
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 5 cut(s) 340, 740, 983, 996, 1130
BspT104I TTCGAA 1 cut(s) 799
BspT107I GGYRCC 2 cut(s) 314, 1199
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 1 cut(s) 1028
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 603, 1377
BssMI GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
BssSI CACGAG 1 cut(s) 1226
BssT1I CCWWGG 1 cut(s) 1377
Bst2BI CACGAG 1 cut(s) 1226
Bst4CI ACNGT 5 cut(s) 319, 391, 406, 514, 1253
Bst6I CTCTTC 2 cut(s) 91, 911
BstAPI GCANNNNNTGC 2 cut(s) 686, 1199
BstBI TTCGAA 1 cut(s) 799
BstC8I GCNNGC 3 cut(s) 346, 376, 1348
BstEII GGTNACC 1 cut(s) 598
BstENI CCTNNNNNAGG 1 cut(s) 133
BstF5I GGATG 6 cut(s) 160, 363, 544, 1180, 1296, 1387
BstFNI CGCG 2 cut(s) 98, 207
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 9 cut(s) 15, 238, 335, 561, 652, 748, 991, 1138, 1269
BstMBI GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
BstMWI GCNNNNNNNGC 9 cut(s) 110, 131, 371, 449, 686, 692, 695, 1040, 1199
BstNSI RCATGY 1 cut(s) 108
BstPI GGTNACC 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 328, 494
BstSFI CTRYAG 1 cut(s) 657
BstSLI GKGCMC 1 cut(s) 1204
BstUI CGCG 2 cut(s) 98, 207
BstX2I RGATCY 3 cut(s) 332, 558, 988
BstXI CCANNNNNNTGG 2 cut(s) 528, 1187
BstYI RGATCY 3 cut(s) 332, 558, 988
BsuRI GGCC 4 cut(s) 200, 1011, 1172, 1179
BtsCI GGATG 6 cut(s) 160, 363, 544, 1180, 1296, 1387
BtsI GCAGTG 2 cut(s) 267, 861
BtsIMutI CAGTG 3 cut(s) 267, 861, 1326
Cac8I GCNNGC 3 cut(s) 346, 376, 1348
CaiI CAGNNNCTG 4 cut(s) 443, 686, 1019, 1304
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 3 cut(s) 817, 855, 1010
CseI GACGC 2 cut(s) 87, 1226
Csp6I GTAC 1 cut(s) 315
CviAII CATG 5 cut(s) 105, 227, 508, 528, 1000
CviQI GTAC 1 cut(s) 315
DpnI GATC 9 cut(s) 14, 237, 334, 560, 651, 747, 990, 1137, 1268
DpnII GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
DraI TTTAAA 1 cut(s) 709
DraIII CACNNNGTG 1 cut(s) 1190
EaeI YGGCCR 2 cut(s) 1170, 1177
Eam1104I CTCTTC 2 cut(s) 91, 911
EarI CTCTTC 2 cut(s) 91, 911
Eco130I CCWWGG 1 cut(s) 1377
Eco47I GGWCC 2 cut(s) 817, 855
Eco57I CTGAAG 1 cut(s) 851
Eco91I GGTNACC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 942
EcoT14I CCWWGG 1 cut(s) 1377
ErhI CCWWGG 1 cut(s) 1377
FaeI CATG 5 cut(s) 108, 230, 511, 531, 1003
FaqI GGGAC 2 cut(s) 868, 1334
FatI CATG 5 cut(s) 104, 226, 507, 527, 999
FokI GGATG 5 cut(s) 167, 370, 551, 1167, 1303
FspBI CTAG 4 cut(s) 345, 821, 876, 1127
GlaI GCGC 1 cut(s) 246
GsaI CCCAGC 2 cut(s) 117, 1016
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 4 cut(s) 200, 1011, 1172, 1179
HapII CCGG 3 cut(s) 330, 395, 495
HgaI GACGC 2 cut(s) 87, 1226
HhaI GCGC 1 cut(s) 247
Hin1II CATG 5 cut(s) 108, 230, 511, 531, 1003
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 6 cut(s) 83, 283, 503, 665, 796, 1358
HpaII CCGG 3 cut(s) 330, 395, 495
HphI GGTGA 2 cut(s) 592, 770
Hpy166II GTNNAC 3 cut(s) 409, 517, 664
Hpy188I TCNGA 5 cut(s) 47, 282, 288, 607, 817
Hpy188III TCNNGA 3 cut(s) 910, 964, 1139
Hpy8I GTNNAC 3 cut(s) 409, 517, 664
Hpy99I CGWCG 3 cut(s) 83, 103, 1242
HpyAV CCTTC 5 cut(s) 19, 60, 283, 527, 593
HpyCH4III ACNGT 5 cut(s) 319, 391, 406, 514, 1253
HpyCH4IV ACGT 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 131, 371, 449, 686, 692, 695, 1040, 1199
HpySE526I ACGT 1 cut(s) 144
Hsp92II CATG 5 cut(s) 108, 230, 511, 531, 1003
HspAI GCGC 1 cut(s) 245
KpnI GGTACC 1 cut(s) 318
Kzo9I GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
LmnI GCTCC 4 cut(s) 331, 491, 643, 899
LweI GCATC 1 cut(s) 1372
MaeI CTAG 4 cut(s) 345, 821, 876, 1127
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 3 cut(s) 598, 772, 1118
MalI GATC 9 cut(s) 14, 237, 334, 560, 651, 747, 990, 1137, 1268
MboI GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
MflI RGATCY 3 cut(s) 332, 558, 988
MhlI GDGCHC 1 cut(s) 1204
MlsI TGGCCA 2 cut(s) 1172, 1179
MluCI AATT 8 cut(s) 577, 717, 871, 942, 948, 1023, 1103, 1366
MluNI TGGCCA 2 cut(s) 1172, 1179
MlyI GAGTC 2 cut(s) 77, 659
MmeI TCCRAC 3 cut(s) 70, 480, 795
MnlI CCTC 5 cut(s) 53, 613, 1081, 1154, 1373
Mox20I TGGCCA 2 cut(s) 1172, 1179
MscI TGGCCA 2 cut(s) 1172, 1179
MseI TTAA 4 cut(s) 543, 708, 843, 1155
MslI CAYNNNNRTG 2 cut(s) 231, 1289
Msp20I TGGCCA 2 cut(s) 1172, 1179
MspA1I CMGCKG 5 cut(s) 113, 683, 1016, 1046, 1304
MspI CCGG 3 cut(s) 330, 395, 495
MspR9I CCNGG 2 cut(s) 330, 496
Mva1269I GAATGC 1 cut(s) 356
MvnI CGCG 2 cut(s) 98, 207
MwoI GCNNNNNNNGC 9 cut(s) 110, 131, 371, 449, 686, 692, 695, 1040, 1199
NciI CCSGG 2 cut(s) 330, 496
NdeII GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 5 cut(s) 108, 230, 511, 531, 1003
NlaIV GGNNCC 6 cut(s) 316, 327, 493, 856, 990, 1201
NmuCI GTSAC 1 cut(s) 598
NspI RCATGY 1 cut(s) 108
NspV TTCGAA 1 cut(s) 799
PctI GAATGC 1 cut(s) 356
PfeI GAWTC 4 cut(s) 283, 503, 796, 1358
PflMI CCANNNNNTGG 2 cut(s) 755, 953
PfoI TCCNGGA 1 cut(s) 328
PleI GAGTC 2 cut(s) 77, 659
PpsI GAGTC 2 cut(s) 77, 659
PspEI GGTNACC 1 cut(s) 598
PspFI CCCAGC 2 cut(s) 113, 1012
PspN4I GGNNCC 6 cut(s) 316, 327, 493, 856, 990, 1201
PspPI GGNCC 3 cut(s) 817, 855, 1010
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 4 cut(s) 443, 686, 1019, 1304
PsuI RGATCY 3 cut(s) 332, 558, 988
PvuII CAGCTG 3 cut(s) 683, 1016, 1046
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 2 cut(s) 231, 1289
SaqAI TTAA 4 cut(s) 543, 708, 843, 1155
Sau3AI GATC 9 cut(s) 12, 235, 332, 558, 649, 745, 988, 1135, 1266
Sau96I GGNCC 3 cut(s) 817, 855, 1010
SchI GAGTC 2 cut(s) 77, 659
ScrFI CCNGG 2 cut(s) 330, 496
SduI GDGCHC 1 cut(s) 1204
SfaNI GCATC 1 cut(s) 1372
SfcI CTRYAG 1 cut(s) 657
SfuI TTCGAA 1 cut(s) 799
SinI GGWCC 2 cut(s) 817, 855
SmiMI CAYNNNNRTG 2 cut(s) 231, 1289
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 8 cut(s) 577, 717, 871, 942, 948, 1023, 1103, 1366
SsiI CCGC 5 cut(s) 96, 111, 207, 210, 1302
SspMI CTAG 4 cut(s) 345, 821, 876, 1127
StyD4I CCNGG 2 cut(s) 328, 494
StyI CCWWGG 1 cut(s) 1377
TaaI ACNGT 5 cut(s) 319, 391, 406, 514, 1253
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 799
TasI AATT 8 cut(s) 577, 717, 871, 942, 948, 1023, 1103, 1366
TauI GCSGC 4 cut(s) 113, 210, 213, 1304
TfiI GAWTC 4 cut(s) 283, 503, 796, 1358
Tru1I TTAA 4 cut(s) 543, 708, 843, 1155
Tru9I TTAA 4 cut(s) 543, 708, 843, 1155
TscAI CASTG 3 cut(s) 267, 868, 1333
TseFI GTSAC 1 cut(s) 598
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 4 cut(s) 729, 929, 1247, 1347
TspRI CASTG 3 cut(s) 267, 868, 1333
Van91I CCANNNNNTGG 2 cut(s) 755, 953
VpaK11BI GGWCC 2 cut(s) 817, 855
XagI CCTNNNNNAGG 1 cut(s) 133
XapI RAATTY 1 cut(s) 942
XceI RCATGY 1 cut(s) 108
XspI CTAG 4 cut(s) 345, 821, 876, 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.