RLG00000010254

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001874
Physical Location & Seq
Forward (+)
64696686 .. 64700081
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010254

Sequence Viewer

Length: 1152 bp
ATGGAGAAAGCGATCAACAGGCAGAAGGTTCTCCTTGACCACCTCCGACCTTCTTCTTCTTCTTCTACCGACCACTCTCCTCTCTCCGCGTCGGCATGTGCCGCTGGGGATAGTGCTGCATATGCAAGGACAAACGTGTTTGGGGATGATGTCGTTATTGTAGCAGCTTATCGCACTCCACTCTGCAAGGCCAAACGCGGTGGCTTCAAAGATACTCATGCCGATGATCTTCTTGCACCTGTTCTCAAGGTTACTCCCTTGCTTTTTGCACTTCTGCTCTTCATGATTCTTCAATATCTCATTGCTTTGGCACTCATACAAGGAGGATGTCTGTCGTTTGCAGAAACTGTGCCGGTTAGAACTGTGAACAGACAATGTTCGTCTGGGCTTCAAGCAATCTTTGAACAAGCCCAGAATTGCCTTCTTCCTATGGGGGTCACCTCGGAAAATGTTGCTCATCGTTTTGGTGTTTCAAGGCAGGAGCAAGATCAGGCTGCAATTGTTGATCCAAAATCTGGTGATGAGAAACCTGTTACAATCTCTGTTGATGATGGGATTCGAAACACAACCTTGTCGGACCTAGCAAAGCTGAAGCCTGTGTTTAAGAAAGATGGGACCACCACTGCTGGTAATTCTAGTCAAGTTAGTGATGGTGCTGGAGCTGTTCTCTTGATGAAGAGAAGTGTTGCCGACCAAAAAGGAATTCCAATTCTTGGTGTATTCAGGAGTTTCGTTGCTGTTGGTGTGGATCCTGCCATCATGGGTGTTGGCCCAGCTGCTGCAATTCCAGTTGCAGTCAAGGCAGCTGGTTTAGAGCTTGATGATATTGACCTTTTTGAGATAAATGAGGCTTTTGCTTCCCAATTTGTGTATTGCCGTAACAAGCTAGGACTTGATCCAGAAAAAATCAATGTTAATGGAGGTGCAATGGCCATTGGCCATCCACTTGGTGCAACAGGTGCCCGTTGTGTTGCCACTCTTTTGCACGAGATGAAGCGTCGGGGAAAAGACTGCCGCTATGGAGTGATCTCAATGTGCATAGGCACAGGGATGGGTGCAGCCGCTGTTTTTGAAAGGGGGGACCGCACTGATGAACTCTGTAATGCTCGCAAGGTTGAATCCCTCAATTTTTTATCCAAGGATGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

384

Amino Acids

40.58

Weight (kDa)

7.99

Isoelectric Point (pI)

27.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 143 - 228 1.2e-20 Thiolase, N-terminal domain
Thiolase_C PF02803 237 - 359 2.3e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 959
AccB7I CCANNNNNTGG 2 cut(s) 515, 713
AccII CGCG 2 cut(s) 89, 198
AciI CCGC 6 cut(s) 87, 102, 198, 1015, 1062, 1084
AclWI GGATC 4 cut(s) 500, 743, 756, 890
AcoI YGGCCR 2 cut(s) 930, 937
AcsI RAATTY 1 cut(s) 702
AcuI CTGAAG 1 cut(s) 611
AdeI CACNNNGTG 1 cut(s) 950
AfiI CCNNNNNNNGG 2 cut(s) 515, 713
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 7 cut(s) 208, 293, 392, 404, 474, 1073, 1118
AjuI GAANNNNNNNTTGG 2 cut(s) 687, 719
AluBI AGCT 7 cut(s) 167, 589, 662, 776, 806, 817, 886
AluI AGCT 7 cut(s) 167, 589, 662, 776, 806, 817, 886
AlwI GGATC 4 cut(s) 500, 743, 756, 890
AlwNI CAGNNNCTG 3 cut(s) 347, 779, 1064
AoxI GGCC 4 cut(s) 189, 769, 930, 937
ApeKI GCWGC 7 cut(s) 116, 164, 494, 776, 779, 803, 1058
ApoI RAATTY 1 cut(s) 702
AspS9I GGNCC 4 cut(s) 577, 615, 770, 1081
AsuHPI GGTGA 2 cut(s) 430, 530
AsuII TTCGAA 1 cut(s) 559
AvaII GGWCC 3 cut(s) 577, 615, 1081
BaeGI GKGCMC 1 cut(s) 964
BalI TGGCCA 2 cut(s) 932, 939
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 959
BauI CACGAG 1 cut(s) 986
BbvI GCAGC 7 cut(s) 103, 176, 481, 763, 766, 815, 1070
BccI CCATC 6 cut(s) 545, 605, 644, 764, 948, 1045
BceAI ACGGC 1 cut(s) 861
BfaI CTAG 3 cut(s) 581, 636, 887
Bme18I GGWCC 3 cut(s) 577, 615, 1081
BmgT120I GGNCC 4 cut(s) 577, 615, 770, 1081
BmiI GGNNCC 4 cut(s) 616, 750, 961, 1082
BmsI GCATC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 651, 683
BpmI CTGGAG 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 441, 1137
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 713
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 1 cut(s) 788
Bse3DI GCAATG 2 cut(s) 300, 933
BseDI CCNNGG 2 cut(s) 441, 1137
BseGI GGATG 5 cut(s) 151, 332, 940, 1056, 1147
BseLI CCNNNNNNNGG 2 cut(s) 515, 713
BseMI GCAATG 2 cut(s) 300, 933
BseNI ACTGG 1 cut(s) 788
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 964
BseXI GCAGC 7 cut(s) 103, 176, 481, 763, 766, 815, 1070
BseYI CCCAGC 2 cut(s) 104, 772
BsgI GTGCAG 1 cut(s) 1077
Bsh1236I CGCG 2 cut(s) 89, 198
BshFI GGCC 4 cut(s) 191, 771, 932, 939
BshNI GGYRCC 1 cut(s) 959
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 2 cut(s) 628, 1094
BslI CCNNNNNNNGG 2 cut(s) 515, 713
BsmFI GGGAC 2 cut(s) 628, 1094
BsnI GGCC 4 cut(s) 191, 771, 932, 939
Bsp119I TTCGAA 1 cut(s) 559
Bsp1286I GDGCHC 1 cut(s) 964
Bsp143I GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
BspACI CCGC 6 cut(s) 87, 102, 198, 1015, 1062, 1084
BspANI GGCC 4 cut(s) 191, 771, 932, 939
BspFNI CGCG 2 cut(s) 89, 198
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 4 cut(s) 616, 750, 961, 1082
BspPI GGATC 4 cut(s) 500, 743, 756, 890
BspQI GCTCTTC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 959
BsrDI GCAATG 2 cut(s) 300, 933
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 1 cut(s) 788
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 441, 1137
BssMI GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
BssSI CACGAG 1 cut(s) 986
BssT1I CCWWGG 1 cut(s) 1137
Bst2BI CACGAG 1 cut(s) 986
Bst4CI ACNGT 2 cut(s) 349, 364
Bst6I CTCTTC 2 cut(s) 284, 671
BstAPI GCANNNNNTGC 1 cut(s) 959
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 1 cut(s) 1108
BstEII GGTNACC 1 cut(s) 436
BstF5I GGATG 5 cut(s) 151, 332, 940, 1056, 1147
BstFNI CGCG 2 cut(s) 89, 198
BstKTI GATC 7 cut(s) 15, 229, 490, 508, 751, 898, 1029
BstMBI GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
BstMWI GCNNNNNNNGC 4 cut(s) 101, 122, 800, 959
BstNSI RCATGY 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 436
BstSLI GKGCMC 1 cut(s) 964
BstUI CGCG 2 cut(s) 89, 198
BstV1I GCAGC 7 cut(s) 103, 176, 481, 763, 766, 815, 1070
BstX2I RGATCY 1 cut(s) 748
BstXI CCANNNNNNTGG 1 cut(s) 947
BstYI RGATCY 1 cut(s) 748
BsuRI GGCC 4 cut(s) 191, 771, 932, 939
BtsCI GGATG 5 cut(s) 151, 332, 940, 1056, 1147
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 2 cut(s) 621, 1086
Cac8I GCNNGC 1 cut(s) 1108
CaiI CAGNNNCTG 3 cut(s) 347, 779, 1064
CciI TCATGA 1 cut(s) 282
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 4 cut(s) 577, 615, 770, 1081
CseI GACGC 2 cut(s) 78, 986
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 4 cut(s) 96, 218, 283, 760
DpnI GATC 7 cut(s) 14, 228, 489, 507, 750, 897, 1028
DpnII GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
DraIII CACNNNGTG 1 cut(s) 950
EaeI YGGCCR 2 cut(s) 930, 937
Eam1104I CTCTTC 2 cut(s) 284, 671
EarI CTCTTC 2 cut(s) 284, 671
Eco130I CCWWGG 1 cut(s) 1137
Eco47I GGWCC 3 cut(s) 577, 615, 1081
Eco57I CTGAAG 1 cut(s) 611
Eco91I GGTNACC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 1137
ErhI CCWWGG 1 cut(s) 1137
FaeI CATG 4 cut(s) 99, 221, 286, 763
FaqI GGGAC 2 cut(s) 628, 1094
FatI CATG 4 cut(s) 95, 217, 282, 759
FauNDI CATATG 1 cut(s) 121
FokI GGATG 4 cut(s) 158, 339, 927, 1063
FspBI CTAG 3 cut(s) 581, 636, 887
GsaI CCCAGC 2 cut(s) 108, 776
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 4 cut(s) 191, 771, 932, 939
HapII CCGG 1 cut(s) 353
HgaI GACGC 2 cut(s) 78, 986
Hin1II CATG 4 cut(s) 99, 221, 286, 763
HinfI GANTC 3 cut(s) 286, 556, 1118
HpaII CCGG 1 cut(s) 353
HphI GGTGA 2 cut(s) 430, 530
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 3 cut(s) 47, 445, 577
Hpy188III TCNNGA 4 cut(s) 283, 670, 724, 899
Hpy8I GTNNAC 1 cut(s) 367
Hpy99I CGWCG 2 cut(s) 94, 1002
HpyAV CCTTC 3 cut(s) 19, 60, 431
HpyCH4III ACNGT 2 cut(s) 349, 364
HpyCH4IV ACGT 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 122, 800, 959
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 4 cut(s) 99, 221, 286, 763
Kzo9I GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 481, 659
Lsp1109I GCAGC 7 cut(s) 103, 176, 481, 763, 766, 815, 1070
LweI GCATC 1 cut(s) 1132
MaeI CTAG 3 cut(s) 581, 636, 887
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 4 cut(s) 250, 436, 532, 878
MalI GATC 7 cut(s) 14, 228, 489, 507, 750, 897, 1028
MboI GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
MboII GAAGA 9 cut(s) 45, 48, 51, 54, 221, 271, 281, 416, 688
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 1 cut(s) 964
MlsI TGGCCA 2 cut(s) 932, 939
MluCI AATT 8 cut(s) 415, 498, 631, 702, 708, 783, 863, 1126
MluNI TGGCCA 2 cut(s) 932, 939
MmeI TCCRAC 2 cut(s) 70, 555
MnlI CCTC 7 cut(s) 53, 90, 317, 451, 841, 914, 1133
Mox20I TGGCCA 2 cut(s) 932, 939
MscI TGGCCA 2 cut(s) 932, 939
MseI TTAA 2 cut(s) 603, 915
MslI CAYNNNNRTG 2 cut(s) 222, 1049
Msp20I TGGCCA 2 cut(s) 932, 939
MspA1I CMGCKG 4 cut(s) 104, 776, 806, 1064
MspI CCGG 1 cut(s) 353
MunI CAATTG 1 cut(s) 498
MvnI CGCG 2 cut(s) 89, 198
MwoI GCNNNNNNNGC 4 cut(s) 101, 122, 800, 959
NdeI CATATG 1 cut(s) 121
NdeII GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
NlaIII CATG 4 cut(s) 99, 221, 286, 763
NlaIV GGNNCC 4 cut(s) 616, 750, 961, 1082
NmuCI GTSAC 1 cut(s) 436
NspI RCATGY 1 cut(s) 99
NspV TTCGAA 1 cut(s) 559
PagI TCATGA 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 286, 556, 1118
PflMI CCANNNNNTGG 2 cut(s) 515, 713
PspEI GGTNACC 1 cut(s) 436
PspFI CCCAGC 2 cut(s) 104, 772
PspN4I GGNNCC 4 cut(s) 616, 750, 961, 1082
PspPI GGNCC 4 cut(s) 577, 615, 770, 1081
PstNI CAGNNNCTG 3 cut(s) 347, 779, 1064
PsuI RGATCY 1 cut(s) 748
PvuII CAGCTG 2 cut(s) 776, 806
RseI CAYNNNNRTG 2 cut(s) 222, 1049
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 603, 915
Sau3AI GATC 7 cut(s) 12, 226, 487, 505, 748, 895, 1026
Sau96I GGNCC 4 cut(s) 577, 615, 770, 1081
SduI GDGCHC 1 cut(s) 964
SfaNI GCATC 1 cut(s) 1132
SfuI TTCGAA 1 cut(s) 559
SinI GGWCC 3 cut(s) 577, 615, 1081
SmiMI CAYNNNNRTG 2 cut(s) 222, 1049
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 8 cut(s) 415, 498, 631, 702, 708, 783, 863, 1126
SsiI CCGC 6 cut(s) 87, 102, 198, 1015, 1062, 1084
SspMI CTAG 3 cut(s) 581, 636, 887
StyI CCWWGG 1 cut(s) 1137
TaaI ACNGT 2 cut(s) 349, 364
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 559
TasI AATT 8 cut(s) 415, 498, 631, 702, 708, 783, 863, 1126
TauI GCSGC 3 cut(s) 104, 1017, 1064
TfiI GAWTC 3 cut(s) 286, 556, 1118
Tru1I TTAA 2 cut(s) 603, 915
Tru9I TTAA 2 cut(s) 603, 915
TscAI CASTG 2 cut(s) 628, 1093
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 7 cut(s) 116, 164, 494, 776, 779, 803, 1058
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 271, 689, 1007, 1107
TspRI CASTG 2 cut(s) 628, 1093
Van91I CCANNNNNTGG 2 cut(s) 515, 713
VpaK11BI GGWCC 3 cut(s) 577, 615, 1081
XapI RAATTY 1 cut(s) 702
XceI RCATGY 1 cut(s) 99
XspI CTAG 3 cut(s) 581, 636, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.