Rmu_sc0003086.1_g000005

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003086.1
OG0001874
Physical Location & Seq
Forward (+)
22977 .. 26446
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003086.1_g000005.1.cds

Sequence Viewer

Length: 1395 bp
atggagaaagcgatcaacaggcagaaggttctccttgaccacctccgaccttcttcttcttcttcttcttcttcttctaccgacgactcttctctctccgcttcggcatgtgccgctggggatagtgctgcctatgcaaggacaaacgtgtttggggatgatgtcgttattgtagcagcttatcgcactccactctgcaaggccaaacgcggcggcttcaaagatactcatgccgatgatcttcttgcacctgttctcaaggcagtgattgagaaaaccaatctgaatccaaaggaagttggggatattgttgtcggtaccgtgttggctcccggatctcaaagagctagcgaatgcaggatggctgctttctatgctggcttccctgaaactgtgccggttagaactgtgaacagacaatgttcgtctgggcttcaagcagttgctgatgtagctgcttctataagagcagggttttatgacattggtattggagccgggttggaatccatgactgtaaacccaatggcatgggaaggggatgttaatcctaaagtaaagatctttgaacaagcccagaattgccttcttcctatgggggtcacctcggaaaatgttgctcatcgttttggtgtttcaaggcaggagcaagatcaggctgcagttgactctcatagaaaggcagctgctgctactgctgctggtagatttaaagatgaaattatccctgtggcaaccaagattgttgatccaaaatctggtgatgagaaacctgttacaatctctgttgatgatgggattcgaaacacaacattgtcggacctagcaaagctgaagcctgtgtttaagaaagatgggaccaccactgctggtaattctagtcaagttagtgatggtgctggagctgttctcttgatgaagagaagtgttgccgaccaaaaaggaattccaattcttggtgtattcaggagtttcgttgctgttggtgtggatcctgccatcatgggtgttggcccagctgctgcaattccagttgcagtcaaggcagctggtttagagcttgatgatattgacctttttgagataaatgaggcttttgcttcccaatttgtgtattgccgtaacaagctaggacttgatccagaaaaaatcaatgttaatggaggtgcattggccattggccatccacttggtgcaacaggtgcccgttgtgttgccactcttttgcacgagatgaagcgtcggggaaaagactgccgctatggagtgatctcaatgtgtatgggcacagggatgggtgcagccgcagtttttgaaaggggggaccgcactgatgaactctgtaatgctcgcaaggttgaatccctcaattttttatccaaggatgctcggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

464

Amino Acids

48.78

Weight (kDa)

7.52

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 317
AccB1I GGYRCC 2 cut(s) 317, 1202
AccB7I CCANNNNNTGG 2 cut(s) 758, 956
AccII CGCG 1 cut(s) 210
AciI CCGC 7 cut(s) 99, 114, 210, 213, 1258, 1305, 1327
AclWI GGATC 5 cut(s) 343, 743, 986, 999, 1133
AcoI YGGCCR 2 cut(s) 1173, 1180
AcsI RAATTY 1 cut(s) 945
AcuI CTGAAG 1 cut(s) 854
AdeI CACNNNGTG 1 cut(s) 1193
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 3 cut(s) 138, 758, 956
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 6 cut(s) 220, 437, 569, 639, 1316, 1361
AjuI GAANNNNNNNTTGG 2 cut(s) 930, 962
AlwI GGATC 5 cut(s) 343, 743, 986, 999, 1133
AlwNI CAGNNNCTG 3 cut(s) 446, 689, 1022
AoxI GGCC 4 cut(s) 201, 1012, 1173, 1180
ApoI RAATTY 1 cut(s) 945
Asp718I GGTACC 1 cut(s) 317
AspS9I GGNCC 4 cut(s) 820, 858, 1013, 1324
AsuC2I CCSGG 2 cut(s) 333, 499
AsuHPI GGTGA 2 cut(s) 595, 773
AsuII TTCGAA 1 cut(s) 802
AsuNHI GCTAGC 1 cut(s) 347
AvaII GGWCC 3 cut(s) 820, 858, 1324
BaeGI GKGCMC 2 cut(s) 1207, 1289
BalI TGGCCA 2 cut(s) 1175, 1182
BamHI GGATCC 1 cut(s) 991
BanI GGYRCC 2 cut(s) 317, 1202
BauI CACGAG 1 cut(s) 1229
BccI CCATC 7 cut(s) 355, 788, 848, 887, 1007, 1191, 1288
BceAI ACGGC 1 cut(s) 1104
BcnI CCSGG 2 cut(s) 333, 499
BfaI CTAG 4 cut(s) 348, 824, 879, 1130
BfmI CTRYAG 1 cut(s) 660
BglII AGATCT 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 333, 499
Bme18I GGWCC 3 cut(s) 820, 858, 1324
BmgT120I GGNCC 4 cut(s) 820, 858, 1013, 1324
BmiI GGNNCC 7 cut(s) 319, 330, 496, 859, 993, 1204, 1325
BmrFI CCNGG 2 cut(s) 333, 499
BmsI GCATC 1 cut(s) 1375
BmtI GCTAGC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 894, 926
BpmI CTGGAG 1 cut(s) 921
Bpu14I TTCGAA 1 cut(s) 802
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 333, 499
BsaBI GATNNNNATC 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 606, 1380
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 758, 956
Bse118I RCCGGY 1 cut(s) 397
Bse1I ACTGG 1 cut(s) 1031
Bse8I GATNNNNATC 1 cut(s) 546
BseDI CCNNGG 2 cut(s) 606, 1380
BseGI GGATG 6 cut(s) 163, 366, 547, 1183, 1299, 1390
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 3 cut(s) 138, 758, 956
BseNI ACTGG 1 cut(s) 1031
BseSI GKGCMC 2 cut(s) 1207, 1289
BseYI CCCAGC 2 cut(s) 116, 1015
BsgI GTGCAG 1 cut(s) 1320
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 4 cut(s) 203, 1014, 1175, 1182
BshNI GGYRCC 2 cut(s) 317, 1202
BsiSI CCGG 3 cut(s) 333, 398, 498
BslFI GGGAC 2 cut(s) 871, 1337
BslI CCNNNNNNNGG 3 cut(s) 138, 758, 956
BsmFI GGGAC 2 cut(s) 871, 1337
BsmI GAATGC 1 cut(s) 359
BsnI GGCC 4 cut(s) 203, 1014, 1175, 1182
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 2 cut(s) 1207, 1289
Bsp143I GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
BspACI CCGC 7 cut(s) 99, 114, 210, 213, 1258, 1305, 1327
BspANI GGCC 4 cut(s) 203, 1014, 1175, 1182
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 7 cut(s) 319, 330, 496, 859, 993, 1204, 1325
BspMAI CTGCAG 1 cut(s) 664
BspOI GCTAGC 1 cut(s) 351
BspPI GGATC 5 cut(s) 343, 743, 986, 999, 1133
BspT104I TTCGAA 1 cut(s) 802
BspT107I GGYRCC 2 cut(s) 317, 1202
BsrFI RCCGGY 1 cut(s) 397
BsrI ACTGG 1 cut(s) 1031
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 2 cut(s) 606, 1380
BssMI GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
BssSI CACGAG 1 cut(s) 1229
BssT1I CCWWGG 1 cut(s) 1380
Bst2BI CACGAG 1 cut(s) 1229
Bst4CI ACNGT 4 cut(s) 322, 394, 409, 517
Bst6I CTCTTC 2 cut(s) 94, 914
BstAPI GCANNNNNTGC 2 cut(s) 689, 1202
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 3 cut(s) 349, 379, 1351
BstEII GGTNACC 1 cut(s) 601
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 6 cut(s) 163, 366, 547, 1183, 1299, 1390
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 9 cut(s) 15, 241, 338, 564, 655, 751, 994, 1141, 1272
BstMBI GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
BstMWI GCNNNNNNNGC 9 cut(s) 113, 134, 374, 452, 689, 695, 698, 1043, 1202
BstNSI RCATGY 1 cut(s) 111
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 2 cut(s) 331, 497
BstSFI CTRYAG 1 cut(s) 660
BstSLI GKGCMC 2 cut(s) 1207, 1289
BstUI CGCG 1 cut(s) 210
BstX2I RGATCY 3 cut(s) 335, 561, 991
BstXI CCANNNNNNTGG 2 cut(s) 531, 1190
BstYI RGATCY 3 cut(s) 335, 561, 991
BsuRI GGCC 4 cut(s) 203, 1014, 1175, 1182
BtsCI GGATG 6 cut(s) 163, 366, 547, 1183, 1299, 1390
BtsI GCAGTG 2 cut(s) 270, 864
BtsIMutI CAGTG 3 cut(s) 270, 864, 1329
Cac8I GCNNGC 3 cut(s) 349, 379, 1351
CaiI CAGNNNCTG 3 cut(s) 446, 689, 1022
Cfr10I RCCGGY 1 cut(s) 397
Cfr13I GGNCC 4 cut(s) 820, 858, 1013, 1324
CseI GACGC 1 cut(s) 1229
Csp6I GTAC 1 cut(s) 318
CviAII CATG 5 cut(s) 108, 230, 511, 531, 1003
CviQI GTAC 1 cut(s) 318
DpnI GATC 9 cut(s) 14, 240, 337, 563, 654, 750, 993, 1140, 1271
DpnII GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
DraI TTTAAA 1 cut(s) 712
DraIII CACNNNGTG 1 cut(s) 1193
EaeI YGGCCR 2 cut(s) 1173, 1180
Eam1104I CTCTTC 2 cut(s) 94, 914
EarI CTCTTC 2 cut(s) 94, 914
Eco130I CCWWGG 1 cut(s) 1380
Eco47I GGWCC 3 cut(s) 820, 858, 1324
Eco57I CTGAAG 1 cut(s) 854
Eco91I GGTNACC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 945
EcoT14I CCWWGG 1 cut(s) 1380
ErhI CCWWGG 1 cut(s) 1380
FaeI CATG 5 cut(s) 111, 233, 514, 534, 1006
FaqI GGGAC 2 cut(s) 871, 1337
FatI CATG 5 cut(s) 107, 229, 510, 530, 1002
FokI GGATG 5 cut(s) 170, 373, 554, 1170, 1306
FspBI CTAG 4 cut(s) 348, 824, 879, 1130
GsaI CCCAGC 2 cut(s) 120, 1019
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 4 cut(s) 203, 1014, 1175, 1182
HapII CCGG 3 cut(s) 333, 398, 498
HgaI GACGC 1 cut(s) 1229
Hin1II CATG 5 cut(s) 111, 233, 514, 534, 1006
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 6 cut(s) 86, 286, 506, 668, 799, 1361
HpaII CCGG 3 cut(s) 333, 398, 498
HphI GGTGA 2 cut(s) 595, 773
Hpy166II GTNNAC 3 cut(s) 412, 520, 667
Hpy188I TCNGA 4 cut(s) 47, 285, 610, 820
Hpy188III TCNNGA 3 cut(s) 913, 967, 1142
Hpy8I GTNNAC 3 cut(s) 412, 520, 667
Hpy99I CGWCG 2 cut(s) 86, 1245
HpyAV CCTTC 4 cut(s) 19, 60, 530, 596
HpyCH4III ACNGT 4 cut(s) 322, 394, 409, 517
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 9 cut(s) 113, 134, 374, 452, 689, 695, 698, 1043, 1202
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 5 cut(s) 111, 233, 514, 534, 1006
KpnI GGTACC 1 cut(s) 321
Kzo9I GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
LmnI GCTCC 4 cut(s) 334, 494, 646, 902
LweI GCATC 1 cut(s) 1375
MaeI CTAG 4 cut(s) 348, 824, 879, 1130
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 601, 775, 1121
MalI GATC 9 cut(s) 14, 240, 337, 563, 654, 750, 993, 1140, 1271
MboI GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
MflI RGATCY 3 cut(s) 335, 561, 991
MhlI GDGCHC 2 cut(s) 1207, 1289
MlsI TGGCCA 2 cut(s) 1175, 1182
MluCI AATT 8 cut(s) 580, 720, 874, 945, 951, 1026, 1106, 1369
MluNI TGGCCA 2 cut(s) 1175, 1182
MlyI GAGTC 2 cut(s) 80, 662
MmeI TCCRAC 3 cut(s) 70, 483, 798
MnlI CCTC 5 cut(s) 53, 616, 1084, 1157, 1376
Mox20I TGGCCA 2 cut(s) 1175, 1182
MscI TGGCCA 2 cut(s) 1175, 1182
MseI TTAA 4 cut(s) 546, 711, 846, 1158
MslI CAYNNNNRTG 2 cut(s) 234, 1292
Msp20I TGGCCA 2 cut(s) 1175, 1182
MspA1I CMGCKG 4 cut(s) 116, 686, 1019, 1049
MspI CCGG 3 cut(s) 333, 398, 498
MspR9I CCNGG 2 cut(s) 333, 499
Mva1269I GAATGC 1 cut(s) 359
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 9 cut(s) 113, 134, 374, 452, 689, 695, 698, 1043, 1202
NciI CCSGG 2 cut(s) 333, 499
NdeII GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
NheI GCTAGC 1 cut(s) 347
NlaIII CATG 5 cut(s) 111, 233, 514, 534, 1006
NlaIV GGNNCC 7 cut(s) 319, 330, 496, 859, 993, 1204, 1325
NmuCI GTSAC 1 cut(s) 601
NspI RCATGY 1 cut(s) 111
NspV TTCGAA 1 cut(s) 802
PctI GAATGC 1 cut(s) 359
PfeI GAWTC 4 cut(s) 286, 506, 799, 1361
PflMI CCANNNNNTGG 2 cut(s) 758, 956
PfoI TCCNGGA 1 cut(s) 331
PleI GAGTC 2 cut(s) 80, 662
PpsI GAGTC 2 cut(s) 80, 662
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 2 cut(s) 116, 1015
PspN4I GGNNCC 7 cut(s) 319, 330, 496, 859, 993, 1204, 1325
PspPI GGNCC 4 cut(s) 820, 858, 1013, 1324
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 3 cut(s) 446, 689, 1022
PsuI RGATCY 3 cut(s) 335, 561, 991
PvuII CAGCTG 3 cut(s) 686, 1019, 1049
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 2 cut(s) 234, 1292
SaqAI TTAA 4 cut(s) 546, 711, 846, 1158
Sau3AI GATC 9 cut(s) 12, 238, 335, 561, 652, 748, 991, 1138, 1269
Sau96I GGNCC 4 cut(s) 820, 858, 1013, 1324
SchI GAGTC 2 cut(s) 80, 662
ScrFI CCNGG 2 cut(s) 333, 499
SduI GDGCHC 2 cut(s) 1207, 1289
SfaNI GCATC 1 cut(s) 1375
SfcI CTRYAG 1 cut(s) 660
SfuI TTCGAA 1 cut(s) 802
SinI GGWCC 3 cut(s) 820, 858, 1324
SmiMI CAYNNNNRTG 2 cut(s) 234, 1292
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 8 cut(s) 580, 720, 874, 945, 951, 1026, 1106, 1369
SsiI CCGC 7 cut(s) 99, 114, 210, 213, 1258, 1305, 1327
SspMI CTAG 4 cut(s) 348, 824, 879, 1130
StyD4I CCNGG 2 cut(s) 331, 497
StyI CCWWGG 1 cut(s) 1380
TaaI ACNGT 4 cut(s) 322, 394, 409, 517
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 802
TasI AATT 8 cut(s) 580, 720, 874, 945, 951, 1026, 1106, 1369
TauI GCSGC 5 cut(s) 116, 213, 216, 1260, 1307
TfiI GAWTC 4 cut(s) 286, 506, 799, 1361
Tru1I TTAA 4 cut(s) 546, 711, 846, 1158
Tru9I TTAA 4 cut(s) 546, 711, 846, 1158
TscAI CASTG 3 cut(s) 270, 871, 1336
TseFI GTSAC 1 cut(s) 601
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 4 cut(s) 732, 932, 1250, 1350
TspRI CASTG 3 cut(s) 270, 871, 1336
Van91I CCANNNNNTGG 2 cut(s) 758, 956
VpaK11BI GGWCC 3 cut(s) 820, 858, 1324
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 1 cut(s) 945
XceI RCATGY 1 cut(s) 111
XspI CTAG 4 cut(s) 348, 824, 879, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.