Rh4DG002800

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001874
Physical Location & Seq
Reverse (-)
525337 .. 529782
4446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG002800.1

Sequence Viewer

Length: 1386 bp
ATGGAGAAAGCGATCAACAGGCAGAAGGTTCTCCTTGACCACCTCCGACCTTCTTCTTCTTCTTCTTCTACCGACGACTCTTCTCTCTCCGCGTCGGCATGTGCCGCTGGGGATAGTGCTGCATATGCAAGGACAAACGTGTTTGGGGATGATGTCGTTATTGTAGCAGCTTATCGCACTCCACTCTGCAAGGCCAAACGCGGTGGCTTCAAAGATACTCATGCCGATGATCTTCTTGCACCTGTTCTCAAGGCAGTGATTGAGAAAACCAATCTGAATCCAAAGGAAGTTGGGGATATTGTTGTCGGTTCCGTGCTGGCTCCCGGATCTCAAAGAGCTAGCGAATGCAGGATGGCTGCTTTCTATGCTGGCTTCCCTGAAACTGTGCCGGTTAGAACTGTGAACAGACAATGTTCGTCTGGGCTTCAAGCAGTTGCTGATGTAGCTGCTTCTATAAGAGCAGGGTTTTATGACATTGGTATTGGAGCTGGGTTGGAATCCATGACTGTAAACCCAATGGCATGGGAAGGGGATGTTAATCCTAAAGTAAAGATCTTTGAACAAGCCCAGAATTGCCTTCTTCCTATGGGGATCACCTCGGAAAATGTTGCTCATCGTTTTGGTGTTTCAAGGCAGGAGCAAGATCAGGCTGCAGTTGACTCTCATAGAAAGGCAGCTGCTGCTACTGCTGCTGGTAGATTTAAAGATGAAATTATCCCTGTGGCAACCAAGATTGTTGATCCAAAATCTGGTGATGAGAAACCTGTTACAATCTCTGTTGATGATGGGATTCGAAACACAACATTGTCGGACCTAGCAAAGCTGAAGCCTGTGTTTAAGAAAGATGGGACCACCACTGCTGGTAATTCTAGTCAAGTTAGTGATGGTGCTGGAGCTGTTCTCTTGATGAAGAGAAGTGTTGCCGACCAAAAAGGAATTCCAATTCTTGGTGTATTCAGGAGTTTCGTTGCTGTTGGTGTGGATCCTGCCATCATGGGTGTTGGCCCAGCTGCTGCAATTCCAGTTGCAGTCAAGGCAGCTGGTTTAGAGCTTGATGATATTGACCTTTTTGAGATAAATGAGGCTTTTGCTTCCCAATTTGTGTATTGCCGTAACAAGCTAGGACTTGATCCAGAAAAAATCAATGTTAATGGAGGTGCAATGGCCATTGGCCATCCACTTGGTGCAACAGGTGCCCGTTGTGTTGCCACTCTTTTGCACGAGATGAAGCGTCGGGGAAAAGACTGCCGCTATGGAGTGATCTCAATGTGCATAGGCACAGGGATGGGTGCAGCTGCTGTTTTTGAAAGGGGGGACCGCACTGATGAACTCTGCAATGCTCGCAAGGTTGAATCCCTCAATTTTTTATCCAAGGATGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

461

Amino Acids

48.51

Weight (kDa)

7.52

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 52 - 306 1e-77 Thiolase, N-terminal domain
Thiolase_C PF02803 315 - 437 3.3e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1193
AccB7I CCANNNNNTGG 2 cut(s) 749, 947
AccII CGCG 2 cut(s) 92, 201
AciI CCGC 5 cut(s) 90, 105, 201, 1249, 1318
AclWI GGATC 6 cut(s) 334, 599, 734, 977, 990, 1124
AcoI YGGCCR 2 cut(s) 1164, 1171
AcsI RAATTY 1 cut(s) 936
AcuI CTGAAG 1 cut(s) 845
AdeI CACNNNGTG 1 cut(s) 1184
AfiI CCNNNNNNNGG 2 cut(s) 749, 947
AflIII ACRYGT 1 cut(s) 138
AgsI TTSAA 6 cut(s) 211, 428, 560, 630, 1307, 1352
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AlwI GGATC 6 cut(s) 334, 599, 734, 977, 990, 1124
AlwNI CAGNNNCTG 4 cut(s) 437, 680, 1013, 1298
AoxI GGCC 4 cut(s) 192, 1003, 1164, 1171
ApoI RAATTY 1 cut(s) 936
AspS9I GGNCC 4 cut(s) 811, 849, 1004, 1315
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 2 cut(s) 586, 764
AsuII TTCGAA 1 cut(s) 793
AsuNHI GCTAGC 1 cut(s) 338
AvaII GGWCC 3 cut(s) 811, 849, 1315
BaeGI GKGCMC 1 cut(s) 1198
BalI TGGCCA 2 cut(s) 1166, 1173
BamHI GGATCC 1 cut(s) 982
BanI GGYRCC 1 cut(s) 1193
BauI CACGAG 1 cut(s) 1220
BccI CCATC 7 cut(s) 346, 779, 839, 878, 998, 1182, 1279
BceAI ACGGC 1 cut(s) 1095
BcnI CCSGG 1 cut(s) 324
BfaI CTAG 4 cut(s) 339, 815, 870, 1121
BfmI CTRYAG 1 cut(s) 651
BglII AGATCT 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 3 cut(s) 811, 849, 1315
BmgT120I GGNCC 4 cut(s) 811, 849, 1004, 1315
BmiI GGNNCC 6 cut(s) 310, 321, 850, 984, 1195, 1316
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 1 cut(s) 1366
BmtI GCTAGC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 885, 917
BpmI CTGGAG 1 cut(s) 912
Bpu14I TTCGAA 1 cut(s) 793
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 597, 1371
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 947
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 1 cut(s) 1022
Bse3DI GCAATG 2 cut(s) 1167, 1342
Bse8I GATNNNNATC 1 cut(s) 537
BseDI CCNNGG 2 cut(s) 597, 1371
BseGI GGATG 6 cut(s) 154, 357, 538, 1174, 1290, 1381
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 2 cut(s) 749, 947
BseMI GCAATG 2 cut(s) 1167, 1342
BseNI ACTGG 1 cut(s) 1022
BseSI GKGCMC 1 cut(s) 1198
BseYI CCCAGC 3 cut(s) 107, 488, 1006
BsgI GTGCAG 1 cut(s) 1311
Bsh1236I CGCG 2 cut(s) 92, 201
BshFI GGCC 4 cut(s) 194, 1005, 1166, 1173
BshNI GGYRCC 1 cut(s) 1193
BsiSI CCGG 2 cut(s) 324, 389
BslFI GGGAC 2 cut(s) 862, 1328
BslI CCNNNNNNNGG 2 cut(s) 749, 947
BsmFI GGGAC 2 cut(s) 862, 1328
BsmI GAATGC 1 cut(s) 350
BsnI GGCC 4 cut(s) 194, 1005, 1166, 1173
Bsp119I TTCGAA 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 1198
BspACI CCGC 5 cut(s) 90, 105, 201, 1249, 1318
BspANI GGCC 4 cut(s) 194, 1005, 1166, 1173
BspFNI CGCG 2 cut(s) 92, 201
BspLI GGNNCC 6 cut(s) 310, 321, 850, 984, 1195, 1316
BspMAI CTGCAG 1 cut(s) 655
BspOI GCTAGC 1 cut(s) 342
BspPI GGATC 6 cut(s) 334, 599, 734, 977, 990, 1124
BspT104I TTCGAA 1 cut(s) 793
BspT107I GGYRCC 1 cut(s) 1193
BsrDI GCAATG 2 cut(s) 1167, 1342
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 1 cut(s) 1022
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 597, 1371
BssSI CACGAG 1 cut(s) 1220
BssT1I CCWWGG 1 cut(s) 1371
Bst2BI CACGAG 1 cut(s) 1220
Bst4CI ACNGT 3 cut(s) 385, 400, 508
Bst6I CTCTTC 2 cut(s) 85, 905
BstAPI GCANNNNNTGC 2 cut(s) 680, 1193
BstBI TTCGAA 1 cut(s) 793
BstC8I GCNNGC 4 cut(s) 318, 340, 370, 1342
BstF5I GGATG 6 cut(s) 154, 357, 538, 1174, 1290, 1381
BstFNI CGCG 2 cut(s) 92, 201
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 1198
BstUI CGCG 2 cut(s) 92, 201
BstX2I RGATCY 3 cut(s) 326, 552, 982
BstXI CCANNNNNNTGG 2 cut(s) 522, 1181
BstYI RGATCY 3 cut(s) 326, 552, 982
BsuRI GGCC 4 cut(s) 194, 1005, 1166, 1173
BtsCI GGATG 6 cut(s) 154, 357, 538, 1174, 1290, 1381
BtsI GCAGTG 2 cut(s) 261, 855
BtsIMutI CAGTG 3 cut(s) 261, 855, 1320
Cac8I GCNNGC 4 cut(s) 318, 340, 370, 1342
CaiI CAGNNNCTG 4 cut(s) 437, 680, 1013, 1298
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 4 cut(s) 811, 849, 1004, 1315
CseI GACGC 2 cut(s) 81, 1220
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 5 cut(s) 99, 221, 502, 522, 994
DraI TTTAAA 1 cut(s) 703
DraIII CACNNNGTG 1 cut(s) 1184
EaeI YGGCCR 2 cut(s) 1164, 1171
Eam1104I CTCTTC 2 cut(s) 85, 905
EarI CTCTTC 2 cut(s) 85, 905
Eco130I CCWWGG 1 cut(s) 1371
Eco47I GGWCC 3 cut(s) 811, 849, 1315
Eco57I CTGAAG 1 cut(s) 845
EcoRI GAATTC 1 cut(s) 936
EcoT14I CCWWGG 1 cut(s) 1371
ErhI CCWWGG 1 cut(s) 1371
FaeI CATG 5 cut(s) 102, 224, 505, 525, 997
FaqI GGGAC 2 cut(s) 862, 1328
FatI CATG 5 cut(s) 98, 220, 501, 521, 993
FauNDI CATATG 1 cut(s) 124
FokI GGATG 5 cut(s) 161, 364, 545, 1161, 1297
FspBI CTAG 4 cut(s) 339, 815, 870, 1121
GsaI CCCAGC 3 cut(s) 111, 492, 1010
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 4 cut(s) 194, 1005, 1166, 1173
HapII CCGG 2 cut(s) 324, 389
HgaI GACGC 2 cut(s) 81, 1220
Hin1II CATG 5 cut(s) 102, 224, 505, 525, 997
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 6 cut(s) 77, 277, 497, 659, 790, 1352
HpaII CCGG 2 cut(s) 324, 389
HphI GGTGA 2 cut(s) 586, 764
Hpy166II GTNNAC 3 cut(s) 403, 511, 658
Hpy188I TCNGA 4 cut(s) 47, 276, 601, 811
Hpy188III TCNNGA 3 cut(s) 904, 958, 1133
Hpy8I GTNNAC 3 cut(s) 403, 511, 658
Hpy99I CGWCG 3 cut(s) 77, 97, 1236
HpyAV CCTTC 4 cut(s) 19, 60, 521, 587
HpyCH4III ACNGT 3 cut(s) 385, 400, 508
HpyCH4IV ACGT 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 5 cut(s) 102, 224, 505, 525, 997
LmnI GCTCC 4 cut(s) 325, 485, 637, 893
LweI GCATC 1 cut(s) 1366
MaeI CTAG 4 cut(s) 339, 815, 870, 1121
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 766, 1112
MboII GAAGA 9 cut(s) 45, 48, 51, 54, 57, 72, 224, 572, 922
MflI RGATCY 3 cut(s) 326, 552, 982
MhlI GDGCHC 1 cut(s) 1198
MlsI TGGCCA 2 cut(s) 1166, 1173
MluCI AATT 8 cut(s) 571, 711, 865, 936, 942, 1017, 1097, 1360
MluNI TGGCCA 2 cut(s) 1166, 1173
MlyI GAGTC 2 cut(s) 71, 653
MmeI TCCRAC 3 cut(s) 70, 474, 789
MnlI CCTC 5 cut(s) 53, 607, 1075, 1148, 1367
Mox20I TGGCCA 2 cut(s) 1166, 1173
MscI TGGCCA 2 cut(s) 1166, 1173
MseI TTAA 4 cut(s) 537, 702, 837, 1149
MslI CAYNNNNRTG 2 cut(s) 225, 1283
Msp20I TGGCCA 2 cut(s) 1166, 1173
MspA1I CMGCKG 5 cut(s) 107, 677, 1010, 1040, 1295
MspI CCGG 2 cut(s) 324, 389
MspR9I CCNGG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 350
MvnI CGCG 2 cut(s) 92, 201
NciI CCSGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 124
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 5 cut(s) 102, 224, 505, 525, 997
NlaIV GGNNCC 6 cut(s) 310, 321, 850, 984, 1195, 1316
NspI RCATGY 1 cut(s) 102
NspV TTCGAA 1 cut(s) 793
PctI GAATGC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 277, 497, 790, 1352
PflMI CCANNNNNTGG 2 cut(s) 749, 947
PfoI TCCNGGA 1 cut(s) 322
PleI GAGTC 2 cut(s) 71, 653
PpsI GAGTC 2 cut(s) 71, 653
PspFI CCCAGC 3 cut(s) 107, 488, 1006
PspN4I GGNNCC 6 cut(s) 310, 321, 850, 984, 1195, 1316
PspPI GGNCC 4 cut(s) 811, 849, 1004, 1315
PstI CTGCAG 1 cut(s) 655
PstNI CAGNNNCTG 4 cut(s) 437, 680, 1013, 1298
PsuI RGATCY 3 cut(s) 326, 552, 982
PvuII CAGCTG 4 cut(s) 677, 1010, 1040, 1295
RseI CAYNNNNRTG 2 cut(s) 225, 1283
SaqAI TTAA 4 cut(s) 537, 702, 837, 1149
Sau96I GGNCC 4 cut(s) 811, 849, 1004, 1315
SchI GAGTC 2 cut(s) 71, 653
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 1 cut(s) 1198
SfaNI GCATC 1 cut(s) 1366
SfcI CTRYAG 1 cut(s) 651
SfuI TTCGAA 1 cut(s) 793
SinI GGWCC 3 cut(s) 811, 849, 1315
SmiMI CAYNNNNRTG 2 cut(s) 225, 1283
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 8 cut(s) 571, 711, 865, 936, 942, 1017, 1097, 1360
SsiI CCGC 5 cut(s) 90, 105, 201, 1249, 1318
SspMI CTAG 4 cut(s) 339, 815, 870, 1121
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 1371
TaaI ACNGT 3 cut(s) 385, 400, 508
TaiI ACGT 1 cut(s) 141
TaqI TCGA 1 cut(s) 793
TasI AATT 8 cut(s) 571, 711, 865, 936, 942, 1017, 1097, 1360
TauI GCSGC 2 cut(s) 107, 1251
TfiI GAWTC 4 cut(s) 277, 497, 790, 1352
Tru1I TTAA 4 cut(s) 537, 702, 837, 1149
Tru9I TTAA 4 cut(s) 537, 702, 837, 1149
TscAI CASTG 3 cut(s) 261, 862, 1327
TspDTI ATGAA 4 cut(s) 723, 923, 1241, 1341
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 3 cut(s) 261, 862, 1327
Van91I CCANNNNNTGG 2 cut(s) 749, 947
VpaK11BI GGWCC 3 cut(s) 811, 849, 1315
XapI RAATTY 1 cut(s) 936
XceI RCATGY 1 cut(s) 102
XspI CTAG 4 cut(s) 339, 815, 870, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.