Rh4AG003100

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001874
Physical Location & Seq
Reverse (-)
544719 .. 548737
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG003100.1

Sequence Viewer

Length: 1035 bp
ATGGCTGCTTTCTATGCTGGCTTCCCTGAAACTGTGCCGGTTAGAACTGTGAACAGACAATGTTCGTCTGGGCTTCAAGCAGTTGCTGATGTAGCTGCTTCTATAAGAGCAGGGTTTTATGACATTGGTATTGGAGCTGGGTTGGAATCCATGACTGCAAACCCAATGGCATGGGAAGGGGATGTTAATCCTAAAGTAAAGATCTTTGAACAAGCCCAGAATTGCCTTCTTCCTATGGGGATCACCTCAGAAAATGTTGCTCATCGTTTTGGTGTTTCAAGGCAGGAGCAAGATCAGGCTGCAGTTGACTCTCATAGAAAGGCAGCTGCTGCTACTGCTTCTGGTAGATTTAAAGATGAAATTATCCCTGTGGCAACCAAGATTGTTGATCCAAAAACTGGTGATGAGAAACCTGTTACAATCTCTGTTGATGATGGGATTCGAAACACAACATTGTCGGACCTAGCAAAGCTGAAGCCTGTGTTTAAGAAAGATGGGACCACCACTGCTGGTAATTCTAGTCAAGTTAGTGATGGTGCTGGAGCTGTTCTCTTGATGAAGAGAAGTGTTGCCGACCAAAAAGGAATTCCAATTCTTGGTGTATTCAGGAGTTTCGTTGCTGTTGGTGTGGATCCTGCCATCATGGGTGTTGGCCCAGCTGCTGCAATTCCAGTTGCAGTCAAGGCAGCTGGTTTAGAGCTTGATGATATTGACCTTTTTGAGATAAATGAGGCTTTTGCTTCCCAATTTGTGTATTGCCGTAACAAGCTAGGACTTGATCCAGAAAAAATCAATGTTAATGGAGGTGCAATGGCCATTGGCCATCCACTTGGTGCAACAGGTGCCCGTTGTGTTGCCACTCTTTTGCACGAGATGAAGCGTCGGGGAAAAGACTGCCGCTATGGAGTGATCTCAATGTGCATAGGCACAGGGATGGGTGCAGCCGCTGTTTTTGAAAGGGGGGACCACACTGATGAACTCTGCAATGCTCGCAAGGTTGACTCCCTCAATTTTTTATCCAAGGATGCTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

344

Amino Acids

36.23

Weight (kDa)

6.54

Isoelectric Point (pI)

23.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 2 - 189 1.2e-58 Thiolase, N-terminal domain
Thiolase_C PF02803 198 - 320 1.8e-48 Thiolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 2 cut(s) 398, 596
AciI CCGC 2 cut(s) 898, 945
AclWI GGATC 5 cut(s) 248, 383, 626, 639, 773
AcoI YGGCCR 2 cut(s) 813, 820
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 494
AdeI CACNNNGTG 1 cut(s) 833
AfiI CCNNNNNNNGG 2 cut(s) 398, 596
AgsI TTSAA 4 cut(s) 77, 209, 279, 956
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
AluBI AGCT 9 cut(s) 95, 137, 326, 472, 545, 659, 689, 700, 769
AluI AGCT 9 cut(s) 95, 137, 326, 472, 545, 659, 689, 700, 769
AlwI GGATC 5 cut(s) 248, 383, 626, 639, 773
AlwNI CAGNNNCTG 4 cut(s) 86, 329, 662, 947
AoxI GGCC 3 cut(s) 652, 813, 820
ApoI RAATTY 1 cut(s) 585
AspS9I GGNCC 4 cut(s) 460, 498, 653, 964
AsuHPI GGTGA 2 cut(s) 235, 413
AsuII TTCGAA 1 cut(s) 442
AvaII GGWCC 3 cut(s) 460, 498, 964
BaeGI GKGCMC 1 cut(s) 847
BalI TGGCCA 2 cut(s) 815, 822
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 842
BauI CACGAG 1 cut(s) 869
BbvI GCAGC 9 cut(s) 82, 286, 313, 316, 335, 646, 649, 698, 953
BccI CCATC 6 cut(s) 428, 488, 527, 647, 831, 928
BceAI ACGGC 1 cut(s) 744
BfaI CTAG 3 cut(s) 464, 519, 770
BfmI CTRYAG 1 cut(s) 300
BglII AGATCT 1 cut(s) 201
Bme18I GGWCC 3 cut(s) 460, 498, 964
BmgT120I GGNCC 4 cut(s) 460, 498, 653, 964
BmiI GGNNCC 4 cut(s) 499, 633, 844, 965
BmsI GCATC 1 cut(s) 1015
BplI GAGNNNNNCTC 2 cut(s) 534, 566
BpmI CTGGAG 1 cut(s) 561
Bpu14I TTCGAA 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 1020
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 596
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 403, 671
Bse3DI GCAATG 2 cut(s) 816, 991
Bse8I GATNNNNATC 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 4 cut(s) 187, 823, 939, 1030
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 2 cut(s) 398, 596
BseMI GCAATG 2 cut(s) 816, 991
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 403, 671
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 9 cut(s) 82, 286, 313, 316, 335, 646, 649, 698, 953
BseYI CCCAGC 2 cut(s) 137, 655
BsgI GTGCAG 1 cut(s) 960
BshFI GGCC 3 cut(s) 654, 815, 822
BshNI GGYRCC 1 cut(s) 842
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 2 cut(s) 511, 977
BslI CCNNNNNNNGG 2 cut(s) 398, 596
BsmFI GGGAC 2 cut(s) 511, 977
BsnI GGCC 3 cut(s) 654, 815, 822
Bsp119I TTCGAA 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 847
Bsp143I GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
BspACI CCGC 2 cut(s) 898, 945
BspANI GGCC 3 cut(s) 654, 815, 822
BspCNI CTCAG 1 cut(s) 260
BspLI GGNNCC 4 cut(s) 499, 633, 844, 965
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 5 cut(s) 248, 383, 626, 639, 773
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 842
BsrDI GCAATG 2 cut(s) 816, 991
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 2 cut(s) 403, 671
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
BssSI CACGAG 1 cut(s) 869
BssT1I CCWWGG 1 cut(s) 1020
Bst2BI CACGAG 1 cut(s) 869
Bst4CI ACNGT 2 cut(s) 34, 49
Bst6I CTCTTC 1 cut(s) 554
BstAPI GCANNNNNTGC 2 cut(s) 329, 842
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 19, 991
BstDEI CTNAG 1 cut(s) 247
BstF5I GGATG 4 cut(s) 187, 823, 939, 1030
BstKTI GATC 7 cut(s) 204, 243, 295, 391, 634, 781, 912
BstMBI GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
BstMWI GCNNNNNNNGC 7 cut(s) 14, 92, 329, 335, 683, 842, 990
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 847
BstV1I GCAGC 9 cut(s) 82, 286, 313, 316, 335, 646, 649, 698, 953
BstX2I RGATCY 2 cut(s) 201, 631
BstXI CCANNNNNNTGG 2 cut(s) 171, 830
BstYI RGATCY 2 cut(s) 201, 631
BsuRI GGCC 3 cut(s) 654, 815, 822
BtsCI GGATG 4 cut(s) 187, 823, 939, 1030
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 504, 969
Cac8I GCNNGC 2 cut(s) 19, 991
CaiI CAGNNNCTG 4 cut(s) 86, 329, 662, 947
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 460, 498, 653, 964
CseI GACGC 1 cut(s) 869
CviAII CATG 3 cut(s) 151, 171, 643
DdeI CTNAG 1 cut(s) 247
DpnI GATC 7 cut(s) 203, 242, 294, 390, 633, 780, 911
DpnII GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
DraI TTTAAA 1 cut(s) 352
DraIII CACNNNGTG 1 cut(s) 833
EaeI YGGCCR 2 cut(s) 813, 820
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 1020
Eco47I GGWCC 3 cut(s) 460, 498, 964
Eco57I CTGAAG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 585
EcoT14I CCWWGG 1 cut(s) 1020
ErhI CCWWGG 1 cut(s) 1020
FaeI CATG 3 cut(s) 154, 174, 646
FaqI GGGAC 2 cut(s) 511, 977
FatI CATG 3 cut(s) 150, 170, 642
FokI GGATG 3 cut(s) 194, 810, 946
FspBI CTAG 3 cut(s) 464, 519, 770
GsaI CCCAGC 2 cut(s) 141, 659
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 3 cut(s) 654, 815, 822
HapII CCGG 1 cut(s) 38
HgaI GACGC 1 cut(s) 869
Hin1II CATG 3 cut(s) 154, 174, 646
HincII GTYRAC 2 cut(s) 307, 1000
HindII GTYRAC 2 cut(s) 307, 1000
HinfI GANTC 4 cut(s) 146, 308, 439, 1001
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 235, 413
Hpy166II GTNNAC 3 cut(s) 52, 307, 1000
Hpy188I TCNGA 2 cut(s) 250, 460
Hpy188III TCNNGA 3 cut(s) 553, 607, 782
Hpy8I GTNNAC 3 cut(s) 52, 307, 1000
Hpy99I CGWCG 1 cut(s) 885
HpyAV CCTTC 2 cut(s) 170, 236
HpyCH4III ACNGT 2 cut(s) 34, 49
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 92, 329, 335, 683, 842, 990
HpyF3I CTNAG 1 cut(s) 247
Hsp92II CATG 3 cut(s) 154, 174, 646
Kzo9I GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
LmnI GCTCC 3 cut(s) 134, 286, 542
Lsp1109I GCAGC 9 cut(s) 82, 286, 313, 316, 335, 646, 649, 698, 953
LweI GCATC 1 cut(s) 1015
MaeI CTAG 3 cut(s) 464, 519, 770
MaeIII GTNAC 2 cut(s) 415, 761
MalI GATC 7 cut(s) 203, 242, 294, 390, 633, 780, 911
MboI GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
MboII GAAGA 2 cut(s) 221, 571
MflI RGATCY 2 cut(s) 201, 631
MhlI GDGCHC 1 cut(s) 847
MlsI TGGCCA 2 cut(s) 815, 822
MluCI AATT 8 cut(s) 220, 360, 514, 585, 591, 666, 746, 1009
MluNI TGGCCA 2 cut(s) 815, 822
MlyI GAGTC 2 cut(s) 302, 995
MmeI TCCRAC 2 cut(s) 123, 438
MnlI CCTC 4 cut(s) 256, 724, 797, 1016
Mox20I TGGCCA 2 cut(s) 815, 822
MscI TGGCCA 2 cut(s) 815, 822
MseI TTAA 4 cut(s) 186, 351, 486, 798
MslI CAYNNNNRTG 2 cut(s) 932, 972
Msp20I TGGCCA 2 cut(s) 815, 822
MspA1I CMGCKG 4 cut(s) 326, 659, 689, 947
MspI CCGG 1 cut(s) 38
MwoI GCNNNNNNNGC 7 cut(s) 14, 92, 329, 335, 683, 842, 990
NdeII GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
NlaIII CATG 3 cut(s) 154, 174, 646
NlaIV GGNNCC 4 cut(s) 499, 633, 844, 965
NspV TTCGAA 1 cut(s) 442
PfeI GAWTC 2 cut(s) 146, 439
PflMI CCANNNNNTGG 2 cut(s) 398, 596
PleI GAGTC 2 cut(s) 302, 995
PpsI GAGTC 2 cut(s) 302, 995
PspFI CCCAGC 2 cut(s) 137, 655
PspN4I GGNNCC 4 cut(s) 499, 633, 844, 965
PspPI GGNCC 4 cut(s) 460, 498, 653, 964
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 4 cut(s) 86, 329, 662, 947
PsuI RGATCY 2 cut(s) 201, 631
PvuII CAGCTG 3 cut(s) 326, 659, 689
RseI CAYNNNNRTG 2 cut(s) 932, 972
SaqAI TTAA 4 cut(s) 186, 351, 486, 798
Sau3AI GATC 7 cut(s) 201, 240, 292, 388, 631, 778, 909
Sau96I GGNCC 4 cut(s) 460, 498, 653, 964
SchI GAGTC 2 cut(s) 302, 995
SduI GDGCHC 1 cut(s) 847
SfaNI GCATC 1 cut(s) 1015
SfcI CTRYAG 1 cut(s) 300
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 3 cut(s) 460, 498, 964
SmiMI CAYNNNNRTG 2 cut(s) 932, 972
Sse9I AATT 8 cut(s) 220, 360, 514, 585, 591, 666, 746, 1009
SsiI CCGC 2 cut(s) 898, 945
SspMI CTAG 3 cut(s) 464, 519, 770
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 2 cut(s) 34, 49
TaqI TCGA 1 cut(s) 442
TasI AATT 8 cut(s) 220, 360, 514, 585, 591, 666, 746, 1009
TauI GCSGC 2 cut(s) 900, 947
TfiI GAWTC 2 cut(s) 146, 439
Tru1I TTAA 4 cut(s) 186, 351, 486, 798
Tru9I TTAA 4 cut(s) 186, 351, 486, 798
TscAI CASTG 2 cut(s) 511, 976
TspDTI ATGAA 4 cut(s) 372, 572, 890, 990
TspRI CASTG 2 cut(s) 511, 976
Van91I CCANNNNNTGG 2 cut(s) 398, 596
VpaK11BI GGWCC 3 cut(s) 460, 498, 964
XapI RAATTY 1 cut(s) 585
XspI CTAG 3 cut(s) 464, 519, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.