Rroxscaffold_5G00355890

Belongs to the thiolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001874
Physical Location & Seq
Forward (+)
35019631 .. 35030878
11248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355890.1

Sequence Viewer

Length: 951 bp
ATGAATTTGCATTATATTCAGGTAAAGATCTTTAAACAAACCCATAATTGCCTTCTTCCTATGGTGGTCACCTCAGAAAATGTTGCTCATTGTTTTACTGTTTCAAGGCAGGAGCAAGATCAGGCTGCAGTTGACTCTCATAGAAAGGTAGTTGTTGTTACTGCTGCTGGTAGATTTAAAGATGAAATTATCCCTGTGGCAACCAAGGTAACTATTTTCCAGGTGCTAGAGTCGAGGGAGGCTGAGGATGGGCCTGGATATGTCTTTGACAAGTTGTTGGTTGTTTGCTTGAGCAGGATTGATGAGAATGATTCAGTAGCTATGATGAGGACTACTAAGGAAGCCTTGGAAGCACAGAAAAATTGGATTGATGATTTTGTGCCACGGAACTTGAGTTCTCCACGAATGGTTGTAAATTCCCGAGCAGATTTCCTGAAGAAGCTGACTTTTAGAGCAACTCTGATGCCAAGCTTTTATCAGGAGCCTCCGCCTCCTAAGGTGGTGCTAAAGAAGAGGCTGCCGATCAAATCCCTAATTCGCTTCACCTGCGTTTCAAAACGCCGGCGCTTCATGATTTTGTCCGACCCAAATTTCGCCAAATCCCAATTCCGGATAGCTTCCGAGATGCAAACCACGAGTCGCGAGCCCTCCATGATTCTCGACCGTGAATTCCTAGACTTTGAGAAACCGATGGATTCCGGAAAGGTTAGGTGTCCATTCAAGCAACCGGGCCTTCATGCCGAATCGTGCTCTGCAATGGTTTGGTATTTGCAGCTTCTAGCAATTCAGATATGTATATCCGGAACCCATCAACTGGCTTCTTCAAAAAAAAAAATTACCGACCCGGGTGCTCTTCCCTCTGCAATTCTATGGTTTTATGGTTTTGGCTATGTGTCCACCATTGACGACTACAAAGTTATTATCAGTTTTGATGTCTCGGGAAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003988 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0006950 GO:0006996 GO:0007031 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009062 GO:0009507 GO:0009514 GO:0009536 GO:0009611 GO:0009657 GO:0009694 GO:0009695 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010111 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016042 GO:0016043 GO:0016053 GO:0016054 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019395 GO:0019752 GO:0023051 GO:0023056 GO:0030258 GO:0031090 GO:0031974 GO:0031981 GO:0032787 GO:0034440 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044283 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046395 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0072329 GO:0072330 GO:0098588 GO:0098805 GO:1901419 GO:1901421 GO:1901575 GO:1901576 GO:1905957 GO:1905959
KEGG Pathways
Metabolic & Signaling
ko00071 ko00280 ko00592 ko01040 ko01100 ko01110 ko01130 ko01212 ko03320 ko04146 map00071 map00280 map00592 map01040 map01100 map01110 map01130 map01212 map03320 map04146
Pfam Domains
Protein Families
Thiolase_C Thiolase_N

Protein Analysis

316

Amino Acids

36.02

Weight (kDa)

9.32

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thiolase_N PF00108 20 - 72 3.3e-12 Thiolase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 554
AccB7I CCANNNNNTGG 1 cut(s) 814
AccII CGCG 1 cut(s) 642
AccIII TCCGGA 3 cut(s) 609, 698, 800
AciI CCGC 1 cut(s) 488
AcsI RAATTY 4 cut(s) 4, 415, 589, 668
AcuI CTGAAG 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 609, 814
AgsI TTSAA 4 cut(s) 105, 555, 721, 825
AjnI CCWGG 2 cut(s) 219, 253
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
AloI GAACNNNNNNTCC 2 cut(s) 379, 411
AluBI AGCT 5 cut(s) 320, 442, 471, 617, 775
AluI AGCT 5 cut(s) 320, 442, 471, 617, 775
Alw21I GWGCWC 2 cut(s) 752, 853
Alw26I GTCTC 1 cut(s) 940
Ama87I CYCGRG 3 cut(s) 420, 844, 937
Aor13HI TCCGGA 3 cut(s) 609, 698, 800
AoxI GGCC 2 cut(s) 251, 730
ApeKI GCWGC 4 cut(s) 125, 164, 517, 772
ApoI RAATTY 4 cut(s) 4, 415, 589, 668
ArsI GACNNNNNNTTYG 4 cut(s) 575, 607, 622, 654
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 251, 730
AsuC2I CCSGG 3 cut(s) 729, 845, 846
AsuHPI GGTGA 2 cut(s) 61, 535
AvaI CYCGRG 3 cut(s) 420, 844, 937
AxyI CCTNAGG 1 cut(s) 495
BanII GRGCYC 1 cut(s) 648
BauI CACGAG 1 cut(s) 634
Bbv12I GWGCWC 2 cut(s) 752, 853
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 112, 151, 504, 784
BccI CCATC 3 cut(s) 242, 685, 816
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 2 cut(s) 221, 255
BcnI CCSGG 3 cut(s) 729, 845, 846
BcoDI GTCTC 1 cut(s) 940
BfaI CTAG 3 cut(s) 227, 674, 779
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 554
BglII AGATCT 1 cut(s) 27
BisI GCNGC 4 cut(s) 126, 165, 518, 773
BlsI GCNGC 4 cut(s) 127, 166, 519, 774
Bme1390I CCNGG 5 cut(s) 221, 255, 729, 845, 846
BmeT110I CYCGRG 3 cut(s) 420, 844, 937
BmgT120I GGNCC 2 cut(s) 251, 730
BmiI GGNNCC 2 cut(s) 483, 805
BmrFI CCNGG 5 cut(s) 221, 255, 729, 845, 846
BmsI GCATC 2 cut(s) 453, 615
Bpu10I CCTNAGC 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 310, 412
BpuMI CCSGG 3 cut(s) 729, 845, 846
BsaJI CCNNGG 4 cut(s) 204, 345, 383, 844
BsaWI WCCGGW 3 cut(s) 609, 698, 800
Bsc4I CCNNNNNNNGG 2 cut(s) 609, 814
Bse118I RCCGGY 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 819
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 1 cut(s) 762
BseAI TCCGGA 3 cut(s) 609, 698, 800
BseBI CCWGG 2 cut(s) 221, 255
BseDI CCNNGG 4 cut(s) 204, 345, 383, 844
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 2 cut(s) 609, 814
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 2 cut(s) 87, 234
BseNI ACTGG 1 cut(s) 819
BseXI GCAGC 4 cut(s) 112, 151, 504, 784
Bsh1236I CGCG 1 cut(s) 642
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 2 cut(s) 253, 732
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 752, 853
BsiHKCI CYCGRG 3 cut(s) 420, 844, 937
BsiSI CCGG 6 cut(s) 562, 610, 699, 728, 801, 845
BslI CCNNNNNNNGG 2 cut(s) 609, 814
BsmAI GTCTC 1 cut(s) 940
BsnI GGCC 2 cut(s) 253, 732
BsoBI CYCGRG 3 cut(s) 420, 844, 937
Bsp1286I GDGCHC 3 cut(s) 648, 752, 853
Bsp13I TCCGGA 3 cut(s) 609, 698, 800
Bsp143I GATC 3 cut(s) 27, 118, 522
Bsp68I TCGCGA 1 cut(s) 642
BspACI CCGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 253, 732
BspCNI CTCAG 2 cut(s) 86, 235
BspEI TCCGGA 3 cut(s) 609, 698, 800
BspFNI CGCG 1 cut(s) 642
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 483, 805
BspMAI CTGCAG 1 cut(s) 130
BspMI ACCTGC 1 cut(s) 554
BspQI GCTCTTC 1 cut(s) 858
BsrDI GCAATG 1 cut(s) 762
BsrFI RCCGGY 1 cut(s) 561
BsrI ACTGG 1 cut(s) 819
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 4 cut(s) 204, 345, 383, 844
BssMI GATC 3 cut(s) 27, 118, 522
BssSI CACGAG 1 cut(s) 634
BssT1I CCWWGG 2 cut(s) 204, 345
Bst2BI CACGAG 1 cut(s) 634
Bst2UI CCWGG 2 cut(s) 221, 255
Bst4CI ACNGT 2 cut(s) 100, 665
Bst6I CTCTTC 2 cut(s) 506, 858
BstC8I GCNNGC 2 cut(s) 563, 644
BstDEI CTNAG 4 cut(s) 73, 243, 336, 495
BstDSI CCRYGG 1 cut(s) 383
BstEII GGTNACC 1 cut(s) 67
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 642
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 3 cut(s) 30, 121, 525
BstMAI GTCTC 1 cut(s) 940
BstMBI GATC 3 cut(s) 27, 118, 522
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 2 cut(s) 350, 546
BstNI CCWGG 2 cut(s) 221, 255
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 5 cut(s) 219, 253, 727, 843, 844
BstSFI CTRYAG 1 cut(s) 126
BstUI CGCG 1 cut(s) 642
BstV1I GCAGC 4 cut(s) 112, 151, 504, 784
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 2 cut(s) 253, 732
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 1 cut(s) 253
BtuMI TCGCGA 1 cut(s) 642
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 2 cut(s) 563, 644
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 2 cut(s) 251, 730
Cfr9I CCCGGG 1 cut(s) 844
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 3 cut(s) 571, 652, 737
DdeI CTNAG 4 cut(s) 73, 243, 336, 495
DpnI GATC 3 cut(s) 29, 120, 524
DpnII GATC 3 cut(s) 27, 118, 522
DraI TTTAAA 2 cut(s) 34, 178
Eam1104I CTCTTC 2 cut(s) 506, 858
EarI CTCTTC 2 cut(s) 506, 858
EciI GGCGGA 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 204, 345
Eco24I GRGCYC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 455
Eco81I CCTNAGG 1 cut(s) 495
Eco88I CYCGRG 3 cut(s) 420, 844, 937
Eco91I GGTNACC 1 cut(s) 67
EcoO65I GGTNACC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 219, 253
EcoT14I CCWWGG 2 cut(s) 204, 345
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 2 cut(s) 204, 345
FaeI CATG 3 cut(s) 574, 655, 740
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FatI CATG 3 cut(s) 570, 651, 736
Fnu4HI GCNGC 4 cut(s) 126, 165, 518, 773
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 4 cut(s) 126, 165, 518, 773
FspBI CTAG 3 cut(s) 227, 674, 779
GlaI GCGC 1 cut(s) 566
GluI GCNGC 4 cut(s) 126, 165, 518, 773
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 2 cut(s) 253, 732
HapII CCGG 6 cut(s) 562, 610, 699, 728, 801, 845
HhaI GCGC 1 cut(s) 567
Hin1II CATG 3 cut(s) 574, 655, 740
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 469
HinfI GANTC 7 cut(s) 134, 230, 311, 637, 655, 695, 743
HpaII CCGG 6 cut(s) 562, 610, 699, 728, 801, 845
HphI GGTGA 2 cut(s) 61, 535
Hpy166II GTNNAC 2 cut(s) 133, 897
Hpy188I TCNGA 5 cut(s) 76, 462, 583, 622, 789
Hpy8I GTNNAC 2 cut(s) 133, 897
HpyAV CCTTC 2 cut(s) 62, 743
HpyCH4III ACNGT 2 cut(s) 100, 665
HpyCH4V TGCA 6 cut(s) 10, 128, 628, 755, 772, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 546
HpyF3I CTNAG 4 cut(s) 73, 243, 336, 495
Hsp92II CATG 3 cut(s) 574, 655, 740
HspAI GCGC 1 cut(s) 565
Kpn2I TCCGGA 3 cut(s) 609, 698, 800
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
Kzo9I GATC 3 cut(s) 27, 118, 522
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 2 cut(s) 112, 481
Lsp1109I GCAGC 4 cut(s) 112, 151, 504, 784
LweI GCATC 2 cut(s) 453, 615
MaeI CTAG 3 cut(s) 227, 674, 779
MaeIII GTNAC 3 cut(s) 67, 157, 208
MalI GATC 3 cut(s) 29, 120, 524
MboI GATC 3 cut(s) 27, 118, 522
MboII GAAGA 5 cut(s) 47, 448, 523, 813, 845
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 3 cut(s) 648, 752, 853
MlyI GAGTC 3 cut(s) 128, 239, 646
MmeI TCCRAC 1 cut(s) 606
MreI CGCCGGCG 1 cut(s) 561
MroI TCCGGA 3 cut(s) 609, 698, 800
MroNI GCCGGC 1 cut(s) 561
MseI TTAA 2 cut(s) 33, 177
MspI CCGG 6 cut(s) 562, 610, 699, 728, 801, 845
MspR9I CCNGG 5 cut(s) 221, 255, 729, 845, 846
MvaI CCWGG 2 cut(s) 221, 255
MvnI CGCG 1 cut(s) 642
MwoI GCNNNNNNNGC 2 cut(s) 350, 546
NaeI GCCGGC 1 cut(s) 563
NciI CCSGG 3 cut(s) 729, 845, 846
NdeII GATC 3 cut(s) 27, 118, 522
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 3 cut(s) 574, 655, 740
NlaIV GGNNCC 2 cut(s) 483, 805
NmuCI GTSAC 1 cut(s) 67
NruI TCGCGA 1 cut(s) 642
PagI TCATGA 1 cut(s) 570
PaqCI CACCTGC 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 858
PdiI GCCGGC 1 cut(s) 563
PfeI GAWTC 4 cut(s) 311, 655, 695, 743
PflMI CCANNNNNTGG 1 cut(s) 814
PkrI GCNGC 4 cut(s) 127, 166, 519, 774
PleI GAGTC 3 cut(s) 128, 238, 645
PpsI GAGTC 3 cut(s) 128, 238, 645
Psp6I CCWGG 2 cut(s) 219, 253
PspEI GGTNACC 1 cut(s) 67
PspGI CCWGG 2 cut(s) 219, 253
PspN4I GGNNCC 2 cut(s) 483, 805
PspPI GGNCC 2 cut(s) 251, 730
PstI CTGCAG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 27
RruI TCGCGA 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 2 cut(s) 33, 177
SatI GCNGC 4 cut(s) 126, 165, 518, 773
Sau3AI GATC 3 cut(s) 27, 118, 522
Sau96I GGNCC 2 cut(s) 251, 730
SchI GAGTC 3 cut(s) 128, 239, 646
ScrFI CCNGG 5 cut(s) 221, 255, 729, 845, 846
SduI GDGCHC 3 cut(s) 648, 752, 853
SfaNI GCATC 2 cut(s) 453, 615
SfcI CTRYAG 1 cut(s) 126
SgrAI CRCCGGYG 1 cut(s) 561
SmaI CCCGGG 1 cut(s) 846
SmlI CTYRAG 2 cut(s) 289, 391
SmoI CTYRAG 2 cut(s) 289, 391
SsiI CCGC 1 cut(s) 488
SspMI CTAG 3 cut(s) 227, 674, 779
StyD4I CCNGG 5 cut(s) 219, 253, 727, 843, 844
StyI CCWWGG 2 cut(s) 204, 345
TaaI ACNGT 2 cut(s) 100, 665
TaqI TCGA 2 cut(s) 233, 660
TfiI GAWTC 4 cut(s) 311, 655, 695, 743
Tru1I TTAA 2 cut(s) 33, 177
Tru9I TTAA 2 cut(s) 33, 177
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 4 cut(s) 125, 164, 517, 772
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 17, 198, 559, 725
TspGWI ACGGA 1 cut(s) 400
TspMI CCCGGG 1 cut(s) 844
Van91I CCANNNNNTGG 1 cut(s) 814
XapI RAATTY 4 cut(s) 4, 415, 589, 668
XmaI CCCGGG 1 cut(s) 844
XspI CTAG 3 cut(s) 227, 674, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.