RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 605 |
| AarI | CACCTGC | 1 cut(s) | 2605 |
| Acc36I | ACCTGC | 1 cut(s) | 2605 |
| Acc65I | GGTACC | 1 cut(s) | 1420 |
| AccB1I | GGYRCC | 1 cut(s) | 1420 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1201 |
| AccBSI | CCGCTC | 1 cut(s) | 2819 |
| AccI | GTMKAC | 1 cut(s) | 2281 |
| AccII | CGCG | 1 cut(s) | 29 |
| AciI | CCGC | 7 cut(s) | 134, 320, 977, 2557, 2640, 2777, 2817 |
| AclWI | GGATC | 3 cut(s) | 1566, 1737, 2916 |
| AcsI | RAATTY | 7 cut(s) | 7, 916, 928, 1094, 1211, 1528, 1654 |
| AcuI | CTGAAG | 4 cut(s) | 65, 1772, 1821, 2892 |
| AfaI | GTAC | 3 cut(s) | 566, 635, 1422 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 334, 436, 1201, 2772, 2783, 2873, 2946 |
| AflII | CTTAAG | 1 cut(s) | 1781 |
| AflIII | ACRYGT | 1 cut(s) | 27 |
| AgeI | ACCGGT | 1 cut(s) | 2832 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2761 |
| AhlI | ACTAGT | 1 cut(s) | 2840 |
| AjnI | CCWGG | 2 cut(s) | 2939, 2945 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2213, 2245 |
| Alw21I | GWGCWC | 2 cut(s) | 1683, 2674 |
| Alw26I | GTCTC | 7 cut(s) | 318, 461, 633, 1813, 1907, 2725, 2874 |
| AlwI | GGATC | 3 cut(s) | 1566, 1737, 2916 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 671, 1667, 2753 |
| AoxI | GGCC | 5 cut(s) | 1995, 2725, 2774, 2942, 2948 |
| ApoI | RAATTY | 7 cut(s) | 7, 916, 928, 1094, 1211, 1528, 1654 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 686, 718 |
| AseI | ATTAAT | 1 cut(s) | 779 |
| AsiGI | ACCGGT | 1 cut(s) | 2832 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 750, 917 |
| Asp718I | GGTACC | 1 cut(s) | 1420 |
| AspLEI | GCGC | 4 cut(s) | 88, 1016, 1926, 2706 |
| AspS9I | GGNCC | 8 cut(s) | 649, 1995, 2726, 2763, 2774, 2861, 2897, 2943 |
| AsuC2I | CCSGG | 2 cut(s) | 1120, 2784 |
| AsuHPI | GGTGA | 3 cut(s) | 433, 1208, 1981 |
| AvaII | GGWCC | 4 cut(s) | 649, 2763, 2861, 2897 |
| AxyI | CCTNAGG | 1 cut(s) | 375 |
| BanI | GGYRCC | 1 cut(s) | 1420 |
| BanII | GRGCYC | 1 cut(s) | 1009 |
| BbsI | GAAGAC | 1 cut(s) | 2134 |
| Bbv12I | GWGCWC | 2 cut(s) | 1683, 2674 |
| BbvCI | CCTCAGC | 1 cut(s) | 1288 |
| BccI | CCATC | 7 cut(s) | 197, 814, 876, 1054, 1541, 2056, 2737 |
| BceAI | ACGGC | 1 cut(s) | 1793 |
| BciT130I | CCWGG | 2 cut(s) | 2941, 2947 |
| BciVI | GTATCC | 1 cut(s) | 1620 |
| BclI | TGATCA | 3 cut(s) | 51, 706, 1498 |
| BcnI | CCSGG | 2 cut(s) | 1120, 2784 |
| BcoDI | GTCTC | 7 cut(s) | 318, 461, 633, 1813, 1907, 2725, 2874 |
| BcuI | ACTAGT | 1 cut(s) | 2840 |
| BfaI | CTAG | 5 cut(s) | 864, 1637, 1748, 2841, 2901 |
| BfmI | CTRYAG | 2 cut(s) | 908, 2420 |
| BfoI | RGCGCY | 2 cut(s) | 89, 2707 |
| BfrI | CTTAAG | 1 cut(s) | 1781 |
| BfuAI | ACCTGC | 1 cut(s) | 2605 |
| BfuI | GTATCC | 1 cut(s) | 1620 |
| BglII | AGATCT | 3 cut(s) | 846, 1186, 2855 |
| BlpI | GCTNAGC | 1 cut(s) | 300 |
| Bme1390I | CCNGG | 4 cut(s) | 1120, 2784, 2941, 2947 |
| Bme18I | GGWCC | 4 cut(s) | 649, 2763, 2861, 2897 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2761 |
| BmgT120I | GGNCC | 8 cut(s) | 649, 1995, 2726, 2763, 2774, 2861, 2897, 2943 |
| BmiI | GGNNCC | 4 cut(s) | 650, 1234, 1422, 2815 |
| BmrFI | CCNGG | 4 cut(s) | 1120, 2784, 2941, 2947 |
| BmrI | ACTGGG | 1 cut(s) | 2806 |
| BmsI | GCATC | 6 cut(s) | 64, 313, 1025, 1178, 1453, 1637 |
| BmuI | ACTGGG | 1 cut(s) | 2806 |
| BpiI | GAAGAC | 1 cut(s) | 2134 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1396, 1428 |
| BpmI | CTGGAG | 3 cut(s) | 1358, 2850, 2923 |
| Bpu10I | CCTNAGC | 2 cut(s) | 198, 1288 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 300 |
| BpuEI | CTTGAG | 5 cut(s) | 158, 350, 506, 1997, 2450 |
| BpuMI | CCSGG | 2 cut(s) | 1120, 2784 |
| BsaI | GGTCTC | 4 cut(s) | 318, 633, 2725, 2874 |
| BsaJI | CCNNGG | 4 cut(s) | 252, 446, 2770, 2945 |
| BsaWI | WCCGGW | 1 cut(s) | 2832 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1953, 1983, 2608, 2638 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 334, 436, 1201, 2772, 2783, 2873, 2946 |
| Bse118I | RCCGGY | 1 cut(s) | 2832 |
| Bse1I | ACTGG | 4 cut(s) | 550, 1911, 2136, 2801 |
| Bse21I | CCTNAGG | 1 cut(s) | 375 |
| BseBI | CCWGG | 2 cut(s) | 2941, 2947 |
| BseDI | CCNNGG | 4 cut(s) | 252, 446, 2770, 2945 |
| BseGI | GGATG | 4 cut(s) | 208, 328, 1016, 1065 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 334, 436, 1201, 2772, 2783, 2873, 2946 |
| BseNI | ACTGG | 4 cut(s) | 550, 1911, 2136, 2801 |
| BseRI | GAGGAG | 6 cut(s) | 347, 1781, 1889, 2333, 2826, 2892 |
| Bsh1236I | CGCG | 1 cut(s) | 29 |
| BshFI | GGCC | 5 cut(s) | 1997, 2727, 2776, 2944, 2950 |
| BshNI | GGYRCC | 1 cut(s) | 1420 |
| BshTI | ACCGGT | 1 cut(s) | 2832 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1683, 2674 |
| BsiSI | CCGG | 3 cut(s) | 1120, 2784, 2833 |
| BslFI | GGGAC | 2 cut(s) | 662, 1601 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 334, 436, 1201, 2772, 2783, 2873, 2946 |
| BsmAI | GTCTC | 7 cut(s) | 318, 461, 633, 1813, 1907, 2725, 2874 |
| BsmFI | GGGAC | 2 cut(s) | 662, 1601 |
| BsnI | GGCC | 5 cut(s) | 1997, 2727, 2776, 2944, 2950 |
| Bso31I | GGTCTC | 4 cut(s) | 318, 633, 2725, 2874 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1009, 1683, 2674 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 300 |
| Bsp19I | CCATGG | 1 cut(s) | 446 |
| BspACI | CCGC | 7 cut(s) | 134, 320, 977, 2557, 2640, 2777, 2817 |
| BspANI | GGCC | 5 cut(s) | 1997, 2727, 2776, 2944, 2950 |
| BspFNI | CGCG | 1 cut(s) | 29 |
| BspHI | TCATGA | 2 cut(s) | 91, 2197 |
| BspLI | GGNNCC | 4 cut(s) | 650, 1234, 1422, 2815 |
| BspMAI | CTGCAG | 2 cut(s) | 912, 2424 |
| BspMI | ACCTGC | 1 cut(s) | 2605 |
| BspPI | GGATC | 3 cut(s) | 1566, 1737, 2916 |
| BspQI | GCTCTTC | 3 cut(s) | 84, 1134, 2562 |
| BspT107I | GGYRCC | 1 cut(s) | 1420 |
| BspTI | CTTAAG | 1 cut(s) | 1781 |
| BspTNI | GGTCTC | 4 cut(s) | 318, 633, 2725, 2874 |
| BsrBI | CCGCTC | 1 cut(s) | 2819 |
| BsrFI | RCCGGY | 1 cut(s) | 2832 |
| BsrI | ACTGG | 4 cut(s) | 550, 1911, 2136, 2801 |
| BssAI | RCCGGY | 1 cut(s) | 2832 |
| BssECI | CCNNGG | 4 cut(s) | 252, 446, 2770, 2945 |
| BssT1I | CCWWGG | 2 cut(s) | 252, 446 |
| Bst2UI | CCWGG | 2 cut(s) | 2941, 2947 |
| Bst4CI | ACNGT | 8 cut(s) | 431, 481, 1284, 1669, 2620, 2763, 2771, 2885 |
| BstAFI | CTTAAG | 1 cut(s) | 1781 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 524, 1835, 2597 |
| BstDSI | CCRYGG | 2 cut(s) | 446, 2770 |
| BstEII | GGTNACC | 1 cut(s) | 439 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 332 |
| BstF5I | GGATG | 4 cut(s) | 208, 328, 1016, 1065 |
| BstFNI | CGCG | 1 cut(s) | 29 |
| BstH2I | RGCGCY | 2 cut(s) | 89, 2707 |
| BstHHI | GCGC | 4 cut(s) | 88, 1016, 1926, 2706 |
| BstMAI | GTCTC | 7 cut(s) | 318, 461, 633, 1813, 1907, 2725, 2874 |
| BstNI | CCWGG | 2 cut(s) | 2941, 2947 |
| BstPI | GGTNACC | 1 cut(s) | 439 |
| BstSCI | CCNGG | 4 cut(s) | 1118, 2782, 2939, 2945 |
| BstSFI | CTRYAG | 2 cut(s) | 908, 2420 |
| BstUI | CGCG | 1 cut(s) | 29 |
| BstV2I | GAAGAC | 1 cut(s) | 2134 |
| BstX2I | RGATCY | 3 cut(s) | 846, 1186, 2855 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2062, 2239 |
| BstYI | RGATCY | 3 cut(s) | 846, 1186, 2855 |
| Bsu36I | CCTNAGG | 1 cut(s) | 375 |
| BsuI | GTATCC | 1 cut(s) | 1620 |
| BsuRI | GGCC | 5 cut(s) | 1997, 2727, 2776, 2944, 2950 |
| BtgI | CCRYGG | 2 cut(s) | 446, 2770 |
| BtgZI | GCGATG | 1 cut(s) | 542 |
| BtsCI | GGATG | 4 cut(s) | 208, 328, 1016, 1065 |
| BtsI | GCAGTG | 1 cut(s) | 2914 |
| BtsIMutI | CAGTG | 3 cut(s) | 406, 2794, 2914 |
| BveI | ACCTGC | 1 cut(s) | 2605 |
| CaiI | CAGNNNCTG | 3 cut(s) | 671, 1667, 2753 |
| CciI | TCATGA | 2 cut(s) | 91, 2197 |
| CfoI | GCGC | 4 cut(s) | 88, 1016, 1926, 2706 |
| Cfr10I | RCCGGY | 1 cut(s) | 2832 |
| Cfr13I | GGNCC | 8 cut(s) | 649, 1995, 2726, 2763, 2774, 2861, 2897, 2943 |
| CseI | GACGC | 3 cut(s) | 948, 1727, 2183 |
| Csp6I | GTAC | 3 cut(s) | 565, 634, 1421 |
| CspAI | ACCGGT | 1 cut(s) | 2832 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1255, 1290 |
| CviQI | GTAC | 3 cut(s) | 565, 634, 1421 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2761 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2761 |
| EciI | GGCGGA | 1 cut(s) | 335 |
| Eco130I | CCWWGG | 2 cut(s) | 252, 446 |
| Eco24I | GRGCYC | 1 cut(s) | 1009 |
| Eco31I | GGTCTC | 4 cut(s) | 318, 633, 2725, 2874 |
| Eco32I | GATATC | 2 cut(s) | 769, 2365 |
| Eco47I | GGWCC | 4 cut(s) | 649, 2763, 2861, 2897 |
| Eco57I | CTGAAG | 4 cut(s) | 65, 1772, 1821, 2892 |
| Eco81I | CCTNAGG | 1 cut(s) | 375 |
| Eco91I | GGTNACC | 1 cut(s) | 439 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 332 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1995 |
| EcoO65I | GGTNACC | 1 cut(s) | 439 |
| EcoRII | CCWGG | 2 cut(s) | 2939, 2945 |
| EcoRV | GATATC | 2 cut(s) | 769, 2365 |
| EcoT14I | CCWWGG | 2 cut(s) | 252, 446 |
| EcoT22I | ATGCAT | 1 cut(s) | 889 |
| EcoT38I | GRGCYC | 1 cut(s) | 1009 |
| ErhI | CCWWGG | 2 cut(s) | 252, 446 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 63, 95, 1098, 1130, 1299, 1331 |
| FaqI | GGGAC | 2 cut(s) | 662, 1601 |
| FauI | CCCGC | 1 cut(s) | 2550 |
| FauNDI | CATATG | 1 cut(s) | 1023 |
| FbaI | TGATCA | 3 cut(s) | 51, 706, 1498 |
| FblI | GTMKAC | 1 cut(s) | 2281 |
| FokI | GGATG | 4 cut(s) | 215, 335, 1003, 1072 |
| FriOI | GRGCYC | 1 cut(s) | 1009 |
| FspBI | CTAG | 5 cut(s) | 864, 1637, 1748, 2841, 2901 |
| GlaI | GCGC | 4 cut(s) | 87, 1015, 1925, 2705 |
| GsuI | CTGGAG | 3 cut(s) | 1358, 2850, 2923 |
| HaeII | RGCGCY | 2 cut(s) | 89, 2707 |
| HaeIII | GGCC | 5 cut(s) | 1997, 2727, 2776, 2944, 2950 |
| HapII | CCGG | 3 cut(s) | 1120, 2784, 2833 |
| HgaI | GACGC | 3 cut(s) | 948, 1727, 2183 |
| HhaI | GCGC | 4 cut(s) | 88, 1016, 1926, 2706 |
| Hin6I | GCGC | 4 cut(s) | 86, 1014, 1924, 2704 |
| HinP1I | GCGC | 4 cut(s) | 86, 1014, 1924, 2704 |
| HincII | GTYRAC | 2 cut(s) | 175, 2282 |
| HindII | GTYRAC | 2 cut(s) | 175, 2282 |
| HindIII | AAGCTT | 7 cut(s) | 747, 1343, 1784, 1899, 2039, 2048, 2505 |
| HinfI | GANTC | 8 cut(s) | 192, 211, 1077, 1240, 1541, 1580, 1857, 2301 |
| HpaII | CCGG | 3 cut(s) | 1120, 2784, 2833 |
| HphI | GGTGA | 3 cut(s) | 433, 1208, 1981 |
| Hpy166II | GTNNAC | 4 cut(s) | 175, 2162, 2282, 2838 |
| Hpy8I | GTNNAC | 4 cut(s) | 175, 2162, 2282, 2838 |
| Hpy99I | CGWCG | 2 cut(s) | 2283, 2763 |
| HpyCH4III | ACNGT | 8 cut(s) | 431, 481, 1284, 1669, 2620, 2763, 2771, 2885 |
| HpyCH4IV | ACGT | 1 cut(s) | 1759 |
| HpySE526I | ACGT | 1 cut(s) | 1759 |
| HspAI | GCGC | 4 cut(s) | 86, 1014, 1924, 2704 |
| KpnI | GGTACC | 1 cut(s) | 1424 |
| Ksp22I | TGATCA | 3 cut(s) | 51, 706, 1498 |
| LguI | GCTCTTC | 3 cut(s) | 84, 1134, 2562 |
| LweI | GCATC | 6 cut(s) | 64, 313, 1025, 1178, 1453, 1637 |
| MaeI | CTAG | 5 cut(s) | 864, 1637, 1748, 2841, 2901 |
| MaeII | ACGT | 1 cut(s) | 1759 |
| MaeIII | GTNAC | 4 cut(s) | 439, 1969, 2204, 2620 |
| MbiI | CCGCTC | 1 cut(s) | 2819 |
| MflI | RGATCY | 3 cut(s) | 846, 1186, 2855 |
| MhlI | GDGCHC | 3 cut(s) | 1009, 1683, 2674 |
| MluI | ACGCGT | 1 cut(s) | 27 |
| MlyI | GAGTC | 2 cut(s) | 1550, 1589 |
| MmeI | TCCRAC | 4 cut(s) | 40, 492, 2611, 2790 |
| Mph1103I | ATGCAT | 1 cut(s) | 889 |
| MroXI | GAANNNNTTC | 2 cut(s) | 750, 917 |
| MseI | TTAA | 7 cut(s) | 153, 453, 779, 894, 932, 1782, 2000 |
| MslI | CAYNNNNRTG | 2 cut(s) | 882, 2202 |
| MspA1I | CMGCKG | 2 cut(s) | 685, 2870 |
| MspCI | CTTAAG | 1 cut(s) | 1781 |
| MspI | CCGG | 3 cut(s) | 1120, 2784, 2833 |
| MspR9I | CCNGG | 4 cut(s) | 1120, 2784, 2941, 2947 |
| MvaI | CCWGG | 2 cut(s) | 2941, 2947 |
| MvnI | CGCG | 1 cut(s) | 29 |
| NciI | CCSGG | 2 cut(s) | 1120, 2784 |
| NcoI | CCATGG | 1 cut(s) | 446 |
| NdeI | CATATG | 1 cut(s) | 1023 |
| NlaIV | GGNNCC | 4 cut(s) | 650, 1234, 1422, 2815 |
| NmeAIII | GCCGAG | 1 cut(s) | 1603 |
| NmuCI | GTSAC | 3 cut(s) | 439, 1969, 2204 |
| NsiI | ATGCAT | 1 cut(s) | 889 |
| PagI | TCATGA | 2 cut(s) | 91, 2197 |
| PaqCI | CACCTGC | 1 cut(s) | 2605 |
| PciSI | GCTCTTC | 3 cut(s) | 84, 1134, 2562 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2767 |
| PdmI | GAANNNNTTC | 2 cut(s) | 750, 917 |
| PfeI | GAWTC | 6 cut(s) | 192, 211, 1077, 1240, 1857, 2301 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1201 |
| PfoI | TCCNGGA | 1 cut(s) | 2782 |
| PinAI | ACCGGT | 1 cut(s) | 2832 |
| PleI | GAGTC | 2 cut(s) | 1549, 1588 |
| PpsI | GAGTC | 2 cut(s) | 1549, 1588 |
| PshBI | ATTAAT | 1 cut(s) | 779 |
| PsiI | TTATAA | 1 cut(s) | 605 |
| Psp6I | CCWGG | 2 cut(s) | 2939, 2945 |
| PspEI | GGTNACC | 1 cut(s) | 439 |
| PspGI | CCWGG | 2 cut(s) | 2939, 2945 |
| PspN4I | GGNNCC | 4 cut(s) | 650, 1234, 1422, 2815 |
| PspPI | GGNCC | 8 cut(s) | 649, 1995, 2726, 2763, 2774, 2861, 2897, 2943 |
| PstI | CTGCAG | 2 cut(s) | 912, 2424 |
| PstNI | CAGNNNCTG | 3 cut(s) | 671, 1667, 2753 |
| PsuI | RGATCY | 3 cut(s) | 846, 1186, 2855 |
| PvuII | CAGCTG | 2 cut(s) | 685, 2870 |
| RsaI | GTAC | 3 cut(s) | 566, 635, 1422 |
| RsaNI | GTAC | 3 cut(s) | 565, 634, 1421 |
| RseI | CAYNNNNRTG | 2 cut(s) | 882, 2202 |
| SalI | GTCGAC | 1 cut(s) | 2280 |
| SapI | GCTCTTC | 3 cut(s) | 84, 1134, 2562 |
| SaqAI | TTAA | 7 cut(s) | 153, 453, 779, 894, 932, 1782, 2000 |
| Sau96I | GGNCC | 8 cut(s) | 649, 1995, 2726, 2763, 2774, 2861, 2897, 2943 |
| SchI | GAGTC | 2 cut(s) | 1550, 1589 |
| ScrFI | CCNGG | 4 cut(s) | 1120, 2784, 2941, 2947 |
| SduI | GDGCHC | 3 cut(s) | 1009, 1683, 2674 |
| SfaNI | GCATC | 6 cut(s) | 64, 313, 1025, 1178, 1453, 1637 |
| SfcI | CTRYAG | 2 cut(s) | 908, 2420 |
| SinI | GGWCC | 4 cut(s) | 649, 2763, 2861, 2897 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 882, 2202 |
| SmlI | CTYRAG | 6 cut(s) | 137, 329, 485, 1781, 2012, 2465 |
| SmoI | CTYRAG | 6 cut(s) | 137, 329, 485, 1781, 2012, 2465 |
| SpeI | ACTAGT | 1 cut(s) | 2840 |
| SsiI | CCGC | 7 cut(s) | 134, 320, 977, 2557, 2640, 2777, 2817 |
| SspI | AATATT | 1 cut(s) | 2256 |
| SspMI | CTAG | 5 cut(s) | 864, 1637, 1748, 2841, 2901 |
| StyD4I | CCNGG | 4 cut(s) | 1118, 2782, 2939, 2945 |
| StyI | CCWWGG | 2 cut(s) | 252, 446 |
| TaaI | ACNGT | 8 cut(s) | 431, 481, 1284, 1669, 2620, 2763, 2771, 2885 |
| TaiI | ACGT | 1 cut(s) | 1762 |
| TaqI | TCGA | 2 cut(s) | 190, 2281 |
| TaqII | GACCGA | 3 cut(s) | 300, 666, 2772 |
| TatI | WGTACW | 2 cut(s) | 564, 633 |
| TauI | GCSGC | 3 cut(s) | 980, 2779, 2819 |
| TfiI | GAWTC | 6 cut(s) | 192, 211, 1077, 1240, 1857, 2301 |
| Tru1I | TTAA | 7 cut(s) | 153, 453, 779, 894, 932, 1782, 2000 |
| Tru9I | TTAA | 7 cut(s) | 153, 453, 779, 894, 932, 1782, 2000 |
| TscAI | CASTG | 3 cut(s) | 406, 2801, 2914 |
| TseFI | GTSAC | 3 cut(s) | 439, 1969, 2204 |
| Tsp45I | GTSAC | 3 cut(s) | 439, 1969, 2204 |
| TspDTI | ATGAA | 6 cut(s) | 60, 80, 1224, 1505, 1520, 1943 |
| TspGWI | ACGGA | 3 cut(s) | 575, 1974, 2324 |
| TspRI | CASTG | 3 cut(s) | 406, 2801, 2914 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1201 |
| Vha464I | CTTAAG | 1 cut(s) | 1781 |
| VpaK11BI | GGWCC | 4 cut(s) | 649, 2763, 2861, 2897 |
| VspI | ATTAAT | 1 cut(s) | 779 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 332 |
| XapI | RAATTY | 7 cut(s) | 7, 916, 928, 1094, 1211, 1528, 1654 |
| XmiI | GTMKAC | 1 cut(s) | 2281 |
| XmnI | GAANNNNTTC | 2 cut(s) | 750, 917 |
| XspI | CTAG | 5 cut(s) | 864, 1637, 1748, 2841, 2901 |
| Zsp2I | ATGCAT | 1 cut(s) | 889 |