RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1360 |
| Acc16I | TGCGCA | 2 cut(s) | 182, 497 |
| Acc36I | ACCTGC | 1 cut(s) | 1360 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1201, 2810 |
| AccBSI | CCGCTC | 1 cut(s) | 756 |
| AccI | GTMKAC | 2 cut(s) | 1497, 1608 |
| AccII | CGCG | 1 cut(s) | 2851 |
| AciI | CCGC | 8 cut(s) | 220, 754, 2227, 2296, 2434, 2700, 2816, 2849 |
| AclI | AACGTT | 1 cut(s) | 2122 |
| AclWI | GGATC | 3 cut(s) | 1194, 1414, 2067 |
| AcoI | YGGCCR | 2 cut(s) | 1989, 2580 |
| AcsI | RAATTY | 6 cut(s) | 7, 846, 1211, 1268, 1571, 1644 |
| AcuI | CTGAAG | 9 cut(s) | 63, 396, 734, 893, 1272, 1326, 1821, 1883, 2412 |
| AcvI | CACGTG | 1 cut(s) | 1010 |
| AfaI | GTAC | 3 cut(s) | 218, 313, 1351 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1201, 1507, 2260, 2457, 2810 |
| AflII | CTTAAG | 1 cut(s) | 931 |
| AflIII | ACRYGT | 1 cut(s) | 1007 |
| AjnI | CCWGG | 7 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1931, 1963 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2658, 2690 |
| Alw26I | GTCTC | 5 cut(s) | 642, 1150, 1323, 1380, 1658 |
| AlwI | GGATC | 3 cut(s) | 1194, 1414, 2067 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1667, 2717 |
| Ama87I | CYCGRG | 1 cut(s) | 1377 |
| AoxI | GGCC | 7 cut(s) | 371, 516, 1989, 2337, 2576, 2580, 2735 |
| ApoI | RAATTY | 6 cut(s) | 7, 846, 1211, 1268, 1571, 1644 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 768, 1647, 2375 |
| AspA2I | CCTAGG | 1 cut(s) | 2678 |
| AspLEI | GCGC | 5 cut(s) | 183, 498, 1050, 2190, 2748 |
| AspS9I | GGNCC | 2 cut(s) | 517, 2835 |
| AsuC2I | CCSGG | 1 cut(s) | 2705 |
| AsuHPI | GGTGA | 3 cut(s) | 274, 1652, 1975 |
| AsuII | TTCGAA | 1 cut(s) | 586 |
| AsuNHI | GCTAGC | 1 cut(s) | 863 |
| AvaI | CYCGRG | 1 cut(s) | 1377 |
| AvaII | GGWCC | 1 cut(s) | 2835 |
| AvrII | CCTAGG | 1 cut(s) | 2678 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1270, 1302 |
| BbrPI | CACGTG | 1 cut(s) | 1010 |
| BbsI | GAAGAC | 1 cut(s) | 2803 |
| BccI | CCATC | 7 cut(s) | 197, 811, 817, 1054, 1203, 1541, 1946 |
| BceAI | ACGGC | 2 cut(s) | 549, 1976 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 66, 100, 1358, 1392, 2306, 2340 |
| BciT130I | CCWGG | 7 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664 |
| BclI | TGATCA | 4 cut(s) | 51, 264, 1000, 1558 |
| BcnI | CCSGG | 1 cut(s) | 2705 |
| BcoDI | GTCTC | 5 cut(s) | 642, 1150, 1323, 1380, 1658 |
| BfaI | CTAG | 8 cut(s) | 699, 864, 1632, 1637, 1698, 2222, 2574, 2679 |
| BfmI | CTRYAG | 4 cut(s) | 472, 1668, 2607, 2709 |
| BfoI | RGCGCY | 2 cut(s) | 2191, 2749 |
| BfrI | CTTAAG | 1 cut(s) | 931 |
| BfuAI | ACCTGC | 1 cut(s) | 1360 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2705 |
| BglII | AGATCT | 4 cut(s) | 379, 489, 1121, 2387 |
| BlnI | CCTAGG | 1 cut(s) | 2678 |
| BlpI | GCTNAGC | 2 cut(s) | 300, 2177 |
| Bme1390I | CCNGG | 8 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664, 2705 |
| Bme18I | GGWCC | 1 cut(s) | 2835 |
| BmeT110I | CYCGRG | 1 cut(s) | 1377 |
| BmgT120I | GGNCC | 2 cut(s) | 517, 2835 |
| BmiI | GGNNCC | 1 cut(s) | 1859 |
| BmrFI | CCNGG | 8 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664, 2705 |
| BmsI | GCATC | 7 cut(s) | 64, 289, 968, 1453, 1637, 2278, 2692 |
| BmtI | GCTAGC | 1 cut(s) | 867 |
| BpiI | GAAGAC | 1 cut(s) | 2803 |
| BpmI | CTGGAG | 3 cut(s) | 1223, 1349, 2369 |
| Bpu10I | CCTNAGC | 2 cut(s) | 804, 2519 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 300, 2177 |
| Bpu14I | TTCGAA | 1 cut(s) | 586 |
| BpuEI | CTTGAG | 4 cut(s) | 306, 506, 1180, 1766 |
| BpuMI | CCSGG | 1 cut(s) | 2705 |
| BsaAI | YACGTR | 2 cut(s) | 311, 1010 |
| BsaI | GGTCTC | 2 cut(s) | 642, 1150 |
| BsaJI | CCNNGG | 8 cut(s) | 198, 252, 315, 446, 1173, 1596, 2678, 2703 |
| BsaWI | WCCGGW | 2 cut(s) | 19, 2596 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1842, 1872, 1887, 1917 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1201, 1507, 2260, 2457, 2810 |
| Bse118I | RCCGGY | 1 cut(s) | 2578 |
| Bse1I | ACTGG | 2 cut(s) | 1146, 2352 |
| Bse3DI | GCAATG | 2 cut(s) | 77, 2367 |
| BseBI | CCWGG | 7 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664 |
| BseDI | CCNNGG | 8 cut(s) | 198, 252, 315, 446, 1173, 1596, 2678, 2703 |
| BseGI | GGATG | 8 cut(s) | 208, 1065, 1422, 1468, 1957, 2235, 2500, 2840 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1201, 1507, 2260, 2457, 2810 |
| BseMI | GCAATG | 2 cut(s) | 77, 2367 |
| BseMII | CTCAG | 7 cut(s) | 282, 291, 380, 663, 1569, 1827, 2168 |
| BseNI | ACTGG | 2 cut(s) | 1146, 2352 |
| BseRI | GAGGAG | 4 cut(s) | 1119, 1605, 1789, 2476 |
| BseYI | CCCAGC | 1 cut(s) | 2749 |
| BsgI | GTGCAG | 3 cut(s) | 651, 963, 2634 |
| Bsh1236I | CGCG | 1 cut(s) | 2851 |
| BshFI | GGCC | 7 cut(s) | 373, 518, 1991, 2339, 2578, 2582, 2737 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1377 |
| BsiSI | CCGG | 4 cut(s) | 20, 2579, 2597, 2705 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1201, 1507, 2260, 2457, 2810 |
| BsmAI | GTCTC | 5 cut(s) | 642, 1150, 1323, 1380, 1658 |
| BsnI | GGCC | 7 cut(s) | 373, 518, 1991, 2339, 2578, 2582, 2737 |
| Bso31I | GGTCTC | 2 cut(s) | 642, 1150 |
| BsoBI | CYCGRG | 1 cut(s) | 1377 |
| Bsp119I | TTCGAA | 1 cut(s) | 586 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 300, 2177 |
| BspACI | CCGC | 8 cut(s) | 220, 754, 2227, 2296, 2434, 2700, 2816, 2849 |
| BspANI | GGCC | 7 cut(s) | 373, 518, 1991, 2339, 2578, 2582, 2737 |
| BspCNI | CTCAG | 7 cut(s) | 283, 292, 381, 664, 1570, 1828, 2169 |
| BspFNI | CGCG | 1 cut(s) | 2851 |
| BspHI | TCATGA | 2 cut(s) | 91, 2146 |
| BspLI | GGNNCC | 1 cut(s) | 1859 |
| BspMAI | CTGCAG | 1 cut(s) | 2713 |
| BspMI | ACCTGC | 1 cut(s) | 1360 |
| BspOI | GCTAGC | 1 cut(s) | 867 |
| BspPI | GGATC | 3 cut(s) | 1194, 1414, 2067 |
| BspQI | GCTCTTC | 2 cut(s) | 1134, 2307 |
| BspT104I | TTCGAA | 1 cut(s) | 586 |
| BspTI | CTTAAG | 1 cut(s) | 931 |
| BspTNI | GGTCTC | 2 cut(s) | 642, 1150 |
| BsrBI | CCGCTC | 1 cut(s) | 756 |
| BsrDI | GCAATG | 2 cut(s) | 77, 2367 |
| BsrFI | RCCGGY | 1 cut(s) | 2578 |
| BsrI | ACTGG | 2 cut(s) | 1146, 2352 |
| BssAI | RCCGGY | 1 cut(s) | 2578 |
| BssECI | CCNNGG | 8 cut(s) | 198, 252, 315, 446, 1173, 1596, 2678, 2703 |
| BssT1I | CCWWGG | 4 cut(s) | 198, 252, 446, 2678 |
| Bst2UI | CCWGG | 7 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664 |
| Bst4CI | ACNGT | 6 cut(s) | 481, 687, 738, 1251, 1317, 1669 |
| BstAFI | CTTAAG | 1 cut(s) | 931 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 277 |
| BstBAI | YACGTR | 2 cut(s) | 311, 1010 |
| BstBI | TTCGAA | 1 cut(s) | 586 |
| BstDEI | CTNAG | 9 cut(s) | 291, 300, 389, 672, 804, 1578, 1836, 2177, 2519 |
| BstEII | GGTNACC | 1 cut(s) | 1963 |
| BstF5I | GGATG | 8 cut(s) | 208, 1065, 1422, 1468, 1957, 2235, 2500, 2840 |
| BstFNI | CGCG | 1 cut(s) | 2851 |
| BstH2I | RGCGCY | 2 cut(s) | 2191, 2749 |
| BstHHI | GCGC | 5 cut(s) | 183, 498, 1050, 2190, 2748 |
| BstMAI | GTCTC | 5 cut(s) | 642, 1150, 1323, 1380, 1658 |
| BstNI | CCWGG | 7 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664 |
| BstNSI | RCATGY | 1 cut(s) | 2282 |
| BstPI | GGTNACC | 1 cut(s) | 1963 |
| BstSCI | CCNGG | 8 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662, 2703 |
| BstSFI | CTRYAG | 4 cut(s) | 472, 1668, 2607, 2709 |
| BstUI | CGCG | 1 cut(s) | 2851 |
| BstV2I | GAAGAC | 1 cut(s) | 2803 |
| BstX2I | RGATCY | 5 cut(s) | 379, 489, 1121, 1186, 2387 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1603, 2056, 2347 |
| BstYI | RGATCY | 5 cut(s) | 379, 489, 1121, 1186, 2387 |
| BsuRI | GGCC | 7 cut(s) | 373, 518, 1991, 2339, 2578, 2582, 2737 |
| BtgZI | GCGATG | 1 cut(s) | 542 |
| BtsCI | GGATG | 8 cut(s) | 208, 1065, 1422, 1468, 1957, 2235, 2500, 2840 |
| BtsI | GCAGTG | 1 cut(s) | 2791 |
| BtsIMutI | CAGTG | 4 cut(s) | 406, 486, 1247, 2791 |
| BveI | ACCTGC | 1 cut(s) | 1360 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1667, 2717 |
| CciI | TCATGA | 2 cut(s) | 91, 2146 |
| CfoI | GCGC | 5 cut(s) | 183, 498, 1050, 2190, 2748 |
| Cfr10I | RCCGGY | 1 cut(s) | 2578 |
| Cfr13I | GGNCC | 2 cut(s) | 517, 2835 |
| Csp6I | GTAC | 3 cut(s) | 217, 312, 1350 |
| CviQI | GTAC | 3 cut(s) | 217, 312, 1350 |
| DdeI | CTNAG | 9 cut(s) | 291, 300, 389, 672, 804, 1578, 1836, 2177, 2519 |
| DraI | TTTAAA | 1 cut(s) | 154 |
| EaeI | YGGCCR | 2 cut(s) | 1989, 2580 |
| EciI | GGCGGA | 1 cut(s) | 2311 |
| Eco130I | CCWWGG | 4 cut(s) | 198, 252, 446, 2678 |
| Eco147I | AGGCCT | 2 cut(s) | 373, 2737 |
| Eco31I | GGTCTC | 2 cut(s) | 642, 1150 |
| Eco47I | GGWCC | 1 cut(s) | 2835 |
| Eco57I | CTGAAG | 9 cut(s) | 63, 396, 734, 893, 1272, 1326, 1821, 1883, 2412 |
| Eco72I | CACGTG | 1 cut(s) | 1010 |
| Eco88I | CYCGRG | 1 cut(s) | 1377 |
| Eco91I | GGTNACC | 1 cut(s) | 1963 |
| EcoO109I | RGGNCCY | 1 cut(s) | 517 |
| EcoO65I | GGTNACC | 1 cut(s) | 1963 |
| EcoRII | CCWGG | 7 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662 |
| EcoT14I | CCWWGG | 4 cut(s) | 198, 252, 446, 2678 |
| EcoT22I | ATGCAT | 3 cut(s) | 79, 282, 889 |
| ErhI | CCWWGG | 4 cut(s) | 198, 252, 446, 2678 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 324, 356, 1175, 1207, 2234, 2266 |
| FauI | CCCGC | 2 cut(s) | 2441, 2809 |
| FauNDI | CATATG | 1 cut(s) | 1503 |
| FbaI | TGATCA | 4 cut(s) | 51, 264, 1000, 1558 |
| FblI | GTMKAC | 2 cut(s) | 1497, 1608 |
| FokI | GGATG | 8 cut(s) | 215, 1072, 1429, 1475, 1964, 2242, 2507, 2827 |
| FseI | GGCCGGCC | 1 cut(s) | 2582 |
| FspBI | CTAG | 8 cut(s) | 699, 864, 1632, 1637, 1698, 2222, 2574, 2679 |
| FspI | TGCGCA | 2 cut(s) | 182, 497 |
| GlaI | GCGC | 5 cut(s) | 182, 497, 1049, 2189, 2747 |
| GsaI | CCCAGC | 1 cut(s) | 2753 |
| GsuI | CTGGAG | 3 cut(s) | 1223, 1349, 2369 |
| HaeII | RGCGCY | 2 cut(s) | 2191, 2749 |
| HaeIII | GGCC | 7 cut(s) | 373, 518, 1991, 2339, 2578, 2582, 2737 |
| HapII | CCGG | 4 cut(s) | 20, 2579, 2597, 2705 |
| HhaI | GCGC | 5 cut(s) | 183, 498, 1050, 2190, 2748 |
| Hin6I | GCGC | 5 cut(s) | 181, 496, 1048, 2188, 2746 |
| HinP1I | GCGC | 5 cut(s) | 181, 496, 1048, 2188, 2746 |
| HincII | GTYRAC | 6 cut(s) | 175, 233, 288, 1254, 1498, 1742 |
| HindII | GTYRAC | 6 cut(s) | 175, 233, 288, 1254, 1498, 1742 |
| HindIII | AAGCTT | 2 cut(s) | 916, 1343 |
| HinfI | GANTC | 9 cut(s) | 211, 610, 1541, 1580, 1769, 1832, 2087, 2332, 2730 |
| HpaII | CCGG | 4 cut(s) | 20, 2579, 2597, 2705 |
| HphI | GGTGA | 3 cut(s) | 274, 1652, 1975 |
| Hpy166II | GTNNAC | 8 cut(s) | 175, 233, 288, 826, 1254, 1498, 1609, 1742 |
| Hpy8I | GTNNAC | 8 cut(s) | 175, 233, 288, 826, 1254, 1498, 1609, 1742 |
| HpyCH4III | ACNGT | 6 cut(s) | 481, 687, 738, 1251, 1317, 1669 |
| HpyCH4IV | ACGT | 6 cut(s) | 310, 1009, 1738, 1759, 2122, 2152 |
| HpyF3I | CTNAG | 9 cut(s) | 291, 300, 389, 672, 804, 1578, 1836, 2177, 2519 |
| HpySE526I | ACGT | 6 cut(s) | 310, 1009, 1738, 1759, 2122, 2152 |
| HspAI | GCGC | 5 cut(s) | 181, 496, 1048, 2188, 2746 |
| KroI | GCCGGC | 1 cut(s) | 2578 |
| KroNI | GCCGGC | 1 cut(s) | 2580 |
| Ksp22I | TGATCA | 4 cut(s) | 51, 264, 1000, 1558 |
| LguI | GCTCTTC | 2 cut(s) | 1134, 2307 |
| LmnI | GCTCC | 8 cut(s) | 163, 1204, 1368, 1863, 2098, 2623, 2632, 2665 |
| LweI | GCATC | 7 cut(s) | 64, 289, 968, 1453, 1637, 2278, 2692 |
| MaeI | CTAG | 8 cut(s) | 699, 864, 1632, 1637, 1698, 2222, 2574, 2679 |
| MaeII | ACGT | 6 cut(s) | 310, 1009, 1738, 1759, 2122, 2152 |
| MaeIII | GTNAC | 3 cut(s) | 439, 1526, 1963 |
| MbiI | CCGCTC | 1 cut(s) | 756 |
| MflI | RGATCY | 5 cut(s) | 379, 489, 1121, 1186, 2387 |
| MlyI | GAGTC | 5 cut(s) | 1550, 1589, 1778, 1841, 2096 |
| MmeI | TCCRAC | 1 cut(s) | 492 |
| Mph1103I | ATGCAT | 3 cut(s) | 79, 282, 889 |
| MroNI | GCCGGC | 1 cut(s) | 2578 |
| MroXI | GAANNNNTTC | 3 cut(s) | 768, 1647, 2375 |
| MseI | TTAA | 9 cut(s) | 153, 792, 873, 921, 932, 953, 1080, 1470, 2036 |
| MslI | CAYNNNNRTG | 4 cut(s) | 882, 1487, 1991, 2151 |
| MspA1I | CMGCKG | 2 cut(s) | 712, 2164 |
| MspCI | CTTAAG | 1 cut(s) | 931 |
| MspI | CCGG | 4 cut(s) | 20, 2579, 2597, 2705 |
| MspR9I | CCNGG | 8 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664, 2705 |
| MvaI | CCWGG | 7 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664 |
| MvnI | CGCG | 1 cut(s) | 2851 |
| NaeI | GCCGGC | 1 cut(s) | 2580 |
| NciI | CCSGG | 1 cut(s) | 2705 |
| NdeI | CATATG | 1 cut(s) | 1503 |
| NgoMIV | GCCGGC | 1 cut(s) | 2578 |
| NheI | GCTAGC | 1 cut(s) | 863 |
| NlaIV | GGNNCC | 1 cut(s) | 1859 |
| NmeAIII | GCCGAG | 1 cut(s) | 2476 |
| NmuCI | GTSAC | 1 cut(s) | 1963 |
| NsbI | TGCGCA | 2 cut(s) | 182, 497 |
| NsiI | ATGCAT | 3 cut(s) | 79, 282, 889 |
| NspI | RCATGY | 1 cut(s) | 2282 |
| NspV | TTCGAA | 1 cut(s) | 586 |
| PaeI | GCATGC | 1 cut(s) | 2282 |
| PagI | TCATGA | 2 cut(s) | 91, 2146 |
| PaqCI | CACCTGC | 1 cut(s) | 1360 |
| PceI | AGGCCT | 2 cut(s) | 373, 2737 |
| PciSI | GCTCTTC | 2 cut(s) | 1134, 2307 |
| PdiI | GCCGGC | 1 cut(s) | 2580 |
| PdmI | GAANNNNTTC | 3 cut(s) | 768, 1647, 2375 |
| PfeI | GAWTC | 4 cut(s) | 211, 610, 2332, 2730 |
| PflFI | GACNNNGTC | 1 cut(s) | 2833 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1201, 2810 |
| PfoI | TCCNGGA | 3 cut(s) | 1200, 2253, 2662 |
| PleI | GAGTC | 5 cut(s) | 1549, 1588, 1777, 1840, 2095 |
| PmaCI | CACGTG | 1 cut(s) | 1010 |
| PmlI | CACGTG | 1 cut(s) | 1010 |
| PpsI | GAGTC | 5 cut(s) | 1549, 1588, 1777, 1840, 2095 |
| Ppu21I | YACGTR | 2 cut(s) | 311, 1010 |
| Psp1406I | AACGTT | 1 cut(s) | 2122 |
| Psp6I | CCWGG | 7 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662 |
| PspCI | CACGTG | 1 cut(s) | 1010 |
| PspEI | GGTNACC | 1 cut(s) | 1963 |
| PspFI | CCCAGC | 1 cut(s) | 2749 |
| PspGI | CCWGG | 7 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662 |
| PspN4I | GGNNCC | 1 cut(s) | 1859 |
| PspPI | GGNCC | 2 cut(s) | 517, 2835 |
| PstI | CTGCAG | 1 cut(s) | 2713 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1667, 2717 |
| PsuI | RGATCY | 5 cut(s) | 379, 489, 1121, 1186, 2387 |
| PsyI | GACNNNGTC | 1 cut(s) | 2833 |
| PvuII | CAGCTG | 2 cut(s) | 712, 2164 |
| RigI | GGCCGGCC | 1 cut(s) | 2582 |
| RsaI | GTAC | 3 cut(s) | 218, 313, 1351 |
| RsaNI | GTAC | 3 cut(s) | 217, 312, 1350 |
| RseI | CAYNNNNRTG | 4 cut(s) | 882, 1487, 1991, 2151 |
| SalI | GTCGAC | 1 cut(s) | 1496 |
| SapI | GCTCTTC | 2 cut(s) | 1134, 2307 |
| SaqAI | TTAA | 9 cut(s) | 153, 792, 873, 921, 932, 953, 1080, 1470, 2036 |
| Sau96I | GGNCC | 2 cut(s) | 517, 2835 |
| SchI | GAGTC | 5 cut(s) | 1550, 1589, 1778, 1841, 2096 |
| ScrFI | CCNGG | 8 cut(s) | 317, 1175, 1202, 1597, 2255, 2341, 2664, 2705 |
| SfaNI | GCATC | 7 cut(s) | 64, 289, 968, 1453, 1637, 2278, 2692 |
| SfcI | CTRYAG | 4 cut(s) | 472, 1668, 2607, 2709 |
| SfuI | TTCGAA | 1 cut(s) | 586 |
| SinI | GGWCC | 1 cut(s) | 2835 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 882, 1487, 1991, 2151 |
| SmlI | CTYRAG | 5 cut(s) | 321, 485, 931, 1159, 1781 |
| SmoI | CTYRAG | 5 cut(s) | 321, 485, 931, 1159, 1781 |
| SphI | GCATGC | 1 cut(s) | 2282 |
| SseBI | AGGCCT | 2 cut(s) | 373, 2737 |
| SsiI | CCGC | 8 cut(s) | 220, 754, 2227, 2296, 2434, 2700, 2816, 2849 |
| SspI | AATATT | 1 cut(s) | 2376 |
| SspMI | CTAG | 8 cut(s) | 699, 864, 1632, 1637, 1698, 2222, 2574, 2679 |
| StuI | AGGCCT | 2 cut(s) | 373, 2737 |
| StyD4I | CCNGG | 8 cut(s) | 315, 1173, 1200, 1595, 2253, 2339, 2662, 2703 |
| StyI | CCWWGG | 4 cut(s) | 198, 252, 446, 2678 |
| TaaI | ACNGT | 6 cut(s) | 481, 687, 738, 1251, 1317, 1669 |
| TaiI | ACGT | 6 cut(s) | 313, 1012, 1741, 1762, 2125, 2155 |
| TaqII | GACCGA | 1 cut(s) | 666 |
| TauI | GCSGC | 1 cut(s) | 2702 |
| TfiI | GAWTC | 4 cut(s) | 211, 610, 2332, 2730 |
| Tru1I | TTAA | 9 cut(s) | 153, 792, 873, 921, 932, 953, 1080, 1470, 2036 |
| Tru9I | TTAA | 9 cut(s) | 153, 792, 873, 921, 932, 953, 1080, 1470, 2036 |
| TscAI | CASTG | 4 cut(s) | 406, 486, 1254, 2791 |
| TseFI | GTSAC | 1 cut(s) | 1963 |
| Tsp45I | GTSAC | 1 cut(s) | 1963 |
| TspGWI | ACGGA | 1 cut(s) | 593 |
| TspRI | CASTG | 4 cut(s) | 406, 486, 1254, 2791 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2833 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1201, 2810 |
| Vha464I | CTTAAG | 1 cut(s) | 931 |
| VpaK11BI | GGWCC | 1 cut(s) | 2835 |
| XapI | RAATTY | 6 cut(s) | 7, 846, 1211, 1268, 1571, 1644 |
| XbaI | TCTAGA | 1 cut(s) | 1631 |
| XceI | RCATGY | 1 cut(s) | 2282 |
| XmaJI | CCTAGG | 1 cut(s) | 2678 |
| XmiI | GTMKAC | 2 cut(s) | 1497, 1608 |
| XmnI | GAANNNNTTC | 3 cut(s) | 768, 1647, 2375 |
| XspI | CTAG | 8 cut(s) | 699, 864, 1632, 1637, 1698, 2222, 2574, 2679 |
| Zsp2I | ATGCAT | 3 cut(s) | 79, 282, 889 |