RchiOBHm_Chr6g0248701

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001063
Physical Location & Seq
Reverse (-)
4642719 .. 4645468
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22292

Sequence Viewer

Length: 1152 bp
ATGATTTCCGCGGCGATTGCGATTCTCACCAGATCTAAAATATGTGTTTGTGGCTCAGATGACAGAACAGTGCTTGAGATCTTGCGCAACAACTCAAAGTTGGAGGCCCTTTATGCGATGACGGCACATCGAGCCTTTCAGTTCTGCAAGCAATACAAACGAACTACGGAGTTTCGAAGGTTATGTGAAATCATCAGGAATCATCTGGCTAATCTGAACAAGTATAGAGATCAAAGAGACCGACCTGACCTATCTGCACCTGAGAGCTTGCAACTGTATCTTGATACTAGATTTGAGCAGCTGAAGATAGCTACTGAACTTGAACTGTGGCAGGAAGCGTTCCGCTCTGTTGAAGATATTCATGGATTGATGTGCATGGTTAAGAAAACCCCTAAACCATCGTTGATGGTGGTTTACTATGCGAAGCTCACAGAAATTTTTTGGATTTCTGCTAGCCACCTTAATCATGCTTATGCATGGTTCAAGCTATTCTCGCTTCAGAAAAGCTTTAACAAAAACTTAAGCCAGAAGGATTTGCAGTTAATAGCATCATCTGTTGTCTCCAAAGGTGTCTTGAGCTGTGCAACCCAGGAAGTAAAGGATCTCTTCCATCTCCTGGAGCATGAATTTCTACCTCTCGACCTTGCTGTAAAGATACAACCACTGTTGACTAAAATCTCAAAATTTGGGGGTAAGCTGGCTTCAGCTTCTTCTGTTCCTGAAGTGCAACTGTCTCAATATGTTCCTGCACTTGAAAAGCTTGGTACTTTGAGGTTGCTCCAGCAGGCGTTTATCCACCCAACTGTTGACGTGGCAGAAATTATCAGGCGTTGGACACATGCTTTACAGCGTATTCATAAACAGTCACTTCATATGGCAAAAGCTAATGAGGGAGAGGGTCCAGAAATTCTAAGAAGTGCGCATGAAGGCAGTTCAAGTGGTCATGCCGAGTCATTAGCTGCAACACTAGCTGAACATCAGCAGCACTTGGTTAGCTTTCAGGTCCTCATTAATCAACTGAAGGAAGTTGCTCCAGCAATACAAAAGTCAATATTAGAATGTACAGATAAAGTAGATAGCATTTCCTCTAATCTGCCTCCAATGACTAAGCATCCTGGCCAGATGGAGAAGGTAACAATCAAGGCACTATGA

Protein Analysis

383

Amino Acids

43.34

Weight (kDa)

9.01

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3a_PCI_TPR-like PF22591 22 - 195 4.5e-66 eIF3a, PCI domain, TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 86, 921
AccB7I CCANNNNNTGG 1 cut(s) 616
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 9, 11, 343
AclWI GGATC 1 cut(s) 609
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 4 cut(s) 435, 626, 683, 906
AcuI CTGAAG 5 cut(s) 323, 482, 687, 741, 1040
AfaI GTAC 2 cut(s) 766, 1063
AfiI CCNNNNNNNGG 1 cut(s) 616
AflII CTTAAG 1 cut(s) 520
AgsI TTSAA 5 cut(s) 323, 353, 484, 755, 936
AjiI CACGTC 1 cut(s) 811
AjnI CCWGG 3 cut(s) 588, 615, 1114
Alw26I GTCTC 3 cut(s) 231, 565, 738
AlwI GGATC 1 cut(s) 609
AoxI GGCC 2 cut(s) 105, 1117
ApeKI GCWGC 3 cut(s) 298, 959, 982
ApoI RAATTY 4 cut(s) 435, 626, 683, 906
AseI ATTAAT 1 cut(s) 1011
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 2 cut(s) 87, 922
AspS9I GGNCC 3 cut(s) 106, 899, 1003
AsuHPI GGTGA 1 cut(s) 19
AsuII TTCGAA 1 cut(s) 175
AsuNHI GCTAGC 1 cut(s) 452
AvaII GGWCC 2 cut(s) 899, 1003
BalI TGGCCA 1 cut(s) 1119
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BbvI GCAGC 3 cut(s) 310, 946, 994
BccI CCATC 4 cut(s) 400, 406, 618, 1117
BceAI ACGGC 1 cut(s) 138
BciT130I CCWGG 3 cut(s) 590, 617, 1116
BcoDI GTCTC 3 cut(s) 231, 565, 738
BfaI CTAG 3 cut(s) 288, 453, 968
BfrI CTTAAG 1 cut(s) 520
BglII AGATCT 2 cut(s) 32, 78
BisI GCNGC 4 cut(s) 12, 299, 960, 983
BlsI GCNGC 4 cut(s) 13, 300, 961, 984
Bme1390I CCNGG 3 cut(s) 590, 617, 1116
Bme18I GGWCC 2 cut(s) 899, 1003
BmgBI CACGTC 1 cut(s) 811
BmgT120I GGNCC 3 cut(s) 106, 899, 1003
BmiI GGNNCC 1 cut(s) 900
BmrFI CCNGG 3 cut(s) 590, 617, 1116
BmsI GCATC 2 cut(s) 557, 1120
BmtI GCTAGC 1 cut(s) 456
BpmI CTGGAG 3 cut(s) 638, 764, 1017
Bpu14I TTCGAA 1 cut(s) 175
BpuEI CTTGAG 2 cut(s) 95, 595
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 9, 588
Bsc4I CCNNNNNNNGG 1 cut(s) 616
BseBI CCWGG 3 cut(s) 590, 617, 1116
BseDI CCNNGG 2 cut(s) 9, 588
BseGI GGATG 1 cut(s) 1111
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMII CTCAG 2 cut(s) 69, 252
BseXI GCAGC 3 cut(s) 310, 946, 994
BsgI GTGCAG 2 cut(s) 240, 732
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 107, 1119
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 3 cut(s) 231, 565, 738
BsnI GGCC 2 cut(s) 107, 1119
Bso31I GGTCTC 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 4 cut(s) 32, 78, 229, 601
BspACI CCGC 3 cut(s) 9, 11, 343
BspANI GGCC 2 cut(s) 107, 1119
BspCNI CTCAG 2 cut(s) 68, 253
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 900
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 1 cut(s) 609
BspT104I TTCGAA 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 345
BsrGI TGTACA 1 cut(s) 1061
BssECI CCNNGG 2 cut(s) 9, 588
BssMI GATC 4 cut(s) 32, 78, 229, 601
Bst2UI CCWGG 3 cut(s) 590, 617, 1116
Bst4CI ACNGT 7 cut(s) 70, 276, 327, 666, 732, 805, 864
Bst6I CTCTTC 1 cut(s) 611
BstAFI CTTAAG 1 cut(s) 520
BstAUI TGTACA 1 cut(s) 1061
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 5 cut(s) 149, 269, 454, 699, 786
BstDEI CTNAG 4 cut(s) 55, 261, 911, 1107
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 1111
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 2 cut(s) 87, 922
BstKTI GATC 4 cut(s) 35, 81, 232, 604
BstMAI GTCTC 3 cut(s) 231, 565, 738
BstMBI GATC 4 cut(s) 32, 78, 229, 601
BstMWI GCNNNNNNNGC 6 cut(s) 17, 113, 122, 131, 493, 968
BstNI CCWGG 3 cut(s) 590, 617, 1116
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 3 cut(s) 588, 615, 1114
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 310, 946, 994
BstX2I RGATCY 3 cut(s) 32, 78, 601
BstYI RGATCY 3 cut(s) 32, 78, 601
BsuRI GGCC 2 cut(s) 107, 1119
BtgI CCRYGG 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 131
BtrI CACGTC 1 cut(s) 811
BtsCI GGATG 1 cut(s) 1111
BtsIMutI CAGTG 2 cut(s) 75, 662
Cac8I GCNNGC 5 cut(s) 149, 269, 454, 699, 786
CfoI GCGC 2 cut(s) 87, 922
Cfr13I GGNCC 3 cut(s) 106, 899, 1003
Cfr42I CCGCGG 1 cut(s) 12
Csp6I GTAC 2 cut(s) 765, 1062
CviAII CATG 8 cut(s) 362, 376, 467, 477, 623, 839, 923, 944
CviQI GTAC 2 cut(s) 765, 1062
DdeI CTNAG 4 cut(s) 55, 261, 911, 1107
DpnI GATC 4 cut(s) 34, 80, 231, 603
DpnII GATC 4 cut(s) 32, 78, 229, 601
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 899, 1003
Eco57I CTGAAG 5 cut(s) 323, 482, 687, 741, 1040
EcoO109I RGGNCCY 2 cut(s) 106, 1003
EcoRII CCWGG 3 cut(s) 588, 615, 1114
EcoT22I ATGCAT 1 cut(s) 478
FaeI CATG 8 cut(s) 365, 379, 470, 480, 626, 842, 926, 947
FalI AAGNNNNNCTT 2 cut(s) 590, 622
FatI CATG 8 cut(s) 361, 375, 466, 476, 622, 838, 922, 943
FauNDI CATATG 1 cut(s) 873
Fnu4HI GCNGC 4 cut(s) 12, 299, 960, 983
FokI GGATG 1 cut(s) 1098
Fsp4HI GCNGC 4 cut(s) 12, 299, 960, 983
FspAI RTGCGCAY 1 cut(s) 921
FspBI CTAG 3 cut(s) 288, 453, 968
FspI TGCGCA 2 cut(s) 86, 921
GlaI GCGC 2 cut(s) 86, 921
GluI GCNGC 4 cut(s) 12, 299, 960, 983
GsuI CTGGAG 3 cut(s) 638, 764, 1017
HaeIII GGCC 2 cut(s) 107, 1119
HhaI GCGC 2 cut(s) 87, 922
Hin1II CATG 8 cut(s) 365, 379, 470, 480, 626, 842, 926, 947
Hin6I GCGC 2 cut(s) 85, 920
HinP1I GCGC 2 cut(s) 85, 920
HincII GTYRAC 2 cut(s) 669, 808
HindII GTYRAC 2 cut(s) 669, 808
HindIII AAGCTT 2 cut(s) 505, 758
HinfI GANTC 3 cut(s) 22, 199, 950
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 3 cut(s) 415, 669, 808
Hpy188I TCNGA 3 cut(s) 58, 216, 501
Hpy188III TCNNGA 6 cut(s) 196, 281, 574, 638, 719, 902
Hpy8I GTNNAC 3 cut(s) 415, 669, 808
HpyAV CCTTC 5 cut(s) 171, 523, 920, 1015, 1123
HpyCH4III ACNGT 7 cut(s) 70, 276, 327, 666, 732, 805, 864
HpyCH4IV ACGT 1 cut(s) 810
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 113, 122, 131, 493, 968
HpyF3I CTNAG 4 cut(s) 55, 261, 911, 1107
HpySE526I ACGT 1 cut(s) 810
Hsp92II CATG 8 cut(s) 365, 379, 470, 480, 626, 842, 926, 947
HspAI GCGC 2 cut(s) 85, 920
KspI CCGCGG 1 cut(s) 12
Kzo9I GATC 4 cut(s) 32, 78, 229, 601
LmnI GCTCC 3 cut(s) 619, 783, 1036
Lsp1109I GCAGC 3 cut(s) 310, 946, 994
LweI GCATC 2 cut(s) 557, 1120
MaeI CTAG 3 cut(s) 288, 453, 968
MaeII ACGT 1 cut(s) 810
MaeIII GTNAC 2 cut(s) 864, 1132
MalI GATC 4 cut(s) 34, 80, 231, 603
MbiI CCGCTC 1 cut(s) 345
MboI GATC 4 cut(s) 32, 78, 229, 601
MboII GAAGA 4 cut(s) 316, 365, 598, 702
MflI RGATCY 3 cut(s) 32, 78, 601
MlsI TGGCCA 1 cut(s) 1119
MluCI AATT 5 cut(s) 435, 626, 683, 819, 906
MluNI TGGCCA 1 cut(s) 1119
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 2 cut(s) 81, 812
MnlI CCTC 8 cut(s) 97, 645, 765, 883, 889, 1016, 1096, 1107
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 1 cut(s) 478
MroXI GAANNNNTTC 1 cut(s) 357
MscI TGGCCA 1 cut(s) 1119
MseI TTAA 6 cut(s) 381, 462, 510, 521, 542, 1011
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 2 cut(s) 11, 301
MspCI CTTAAG 1 cut(s) 520
MspR9I CCNGG 3 cut(s) 590, 617, 1116
MvaI CCWGG 3 cut(s) 590, 617, 1116
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 6 cut(s) 17, 113, 122, 131, 493, 968
NdeI CATATG 1 cut(s) 873
NdeII GATC 4 cut(s) 32, 78, 229, 601
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 8 cut(s) 365, 379, 470, 480, 626, 842, 926, 947
NlaIV GGNNCC 1 cut(s) 900
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 1 cut(s) 864
NsbI TGCGCA 2 cut(s) 86, 921
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 1 cut(s) 842
NspV TTCGAA 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 22, 199
PflMI CCANNNNNTGG 1 cut(s) 616
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 4 cut(s) 13, 300, 961, 984
PleI GAGTC 1 cut(s) 958
PpsI GAGTC 1 cut(s) 958
PpuMI RGGWCCY 1 cut(s) 1003
PshBI ATTAAT 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 1003
Psp6I CCWGG 3 cut(s) 588, 615, 1114
PspGI CCWGG 3 cut(s) 588, 615, 1114
PspN4I GGNNCC 1 cut(s) 900
PspPI GGNCC 3 cut(s) 106, 899, 1003
PspPPI RGGWCCY 1 cut(s) 1003
PsuI RGATCY 3 cut(s) 32, 78, 601
PvuII CAGCTG 1 cut(s) 301
RsaI GTAC 2 cut(s) 766, 1063
RsaNI GTAC 2 cut(s) 765, 1062
RseI CAYNNNNRTG 1 cut(s) 471
SacII CCGCGG 1 cut(s) 12
SaqAI TTAA 6 cut(s) 381, 462, 510, 521, 542, 1011
SatI GCNGC 4 cut(s) 12, 299, 960, 983
Sau3AI GATC 4 cut(s) 32, 78, 229, 601
Sau96I GGNCC 3 cut(s) 106, 899, 1003
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 3 cut(s) 590, 617, 1116
SfaNI GCATC 2 cut(s) 557, 1120
Sfr303I CCGCGG 1 cut(s) 12
SfuI TTCGAA 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 12
SinI GGWCC 2 cut(s) 899, 1003
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 3 cut(s) 74, 520, 574
SmoI CTYRAG 3 cut(s) 74, 520, 574
Sse9I AATT 5 cut(s) 435, 626, 683, 819, 906
SsiI CCGC 3 cut(s) 9, 11, 343
SspI AATATT 1 cut(s) 1053
SspMI CTAG 3 cut(s) 288, 453, 968
StyD4I CCNGG 3 cut(s) 588, 615, 1114
TaaI ACNGT 7 cut(s) 70, 276, 327, 666, 732, 805, 864
TaiI ACGT 1 cut(s) 813
TaqI TCGA 3 cut(s) 130, 175, 639
TaqII GACCGA 1 cut(s) 255
TasI AATT 5 cut(s) 435, 626, 683, 819, 906
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 22, 199
Tru1I TTAA 6 cut(s) 381, 462, 510, 521, 542, 1011
Tru9I TTAA 6 cut(s) 381, 462, 510, 521, 542, 1011
TscAI CASTG 2 cut(s) 75, 669
TseFI GTSAC 1 cut(s) 864
TseI GCWGC 3 cut(s) 298, 959, 982
Tsp45I GTSAC 1 cut(s) 864
TspDTI ATGAA 5 cut(s) 350, 639, 845, 860, 939
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 2 cut(s) 75, 669
Van91I CCANNNNNTGG 1 cut(s) 616
Vha464I CTTAAG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 899, 1003
VspI ATTAAT 1 cut(s) 1011
XapI RAATTY 4 cut(s) 435, 626, 683, 906
XceI RCATGY 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 3 cut(s) 288, 453, 968
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.