Belongs to the glycosyltransferase 2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1453 |
| Acc36I | ACCTGC | 2 cut(s) | 215, 1453 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 640 |
| AccBSI | CCGCTC | 1 cut(s) | 2054 |
| AccI | GTMKAC | 4 cut(s) | 765, 879, 2077, 2213 |
| AccIII | TCCGGA | 1 cut(s) | 1435 |
| AciI | CCGC | 3 cut(s) | 1295, 1498, 2052 |
| AclI | AACGTT | 2 cut(s) | 147, 492 |
| AclWI | GGATC | 5 cut(s) | 50, 1433, 1446, 1452, 1465 |
| AcsI | RAATTY | 6 cut(s) | 462, 1210, 1577, 1978, 1988, 1994 |
| AcuI | CTGAAG | 1 cut(s) | 249 |
| AfaI | GTAC | 5 cut(s) | 590, 1328, 1361, 1711, 1782 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 640, 1408, 1472, 1552, 1553, 1952 |
| AflIII | ACRYGT | 2 cut(s) | 867, 888 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 676, 708 |
| AleI | CACNNNNGTG | 1 cut(s) | 1724 |
| Alw21I | GWGCWC | 1 cut(s) | 508 |
| Alw26I | GTCTC | 4 cut(s) | 732, 844, 1745, 1913 |
| AlwI | GGATC | 5 cut(s) | 50, 1433, 1446, 1452, 1465 |
| Ama87I | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| Aor13HI | TCCGGA | 1 cut(s) | 1435 |
| AoxI | GGCC | 2 cut(s) | 91, 1605 |
| ApaI | GGGCCC | 1 cut(s) | 1609 |
| ApeKI | GCWGC | 2 cut(s) | 1495, 2228 |
| ApoI | RAATTY | 6 cut(s) | 462, 1210, 1577, 1978, 1988, 1994 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 641, 673, 1005, 1037 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 264, 1150 |
| AspS9I | GGNCC | 4 cut(s) | 1064, 1605, 1606, 1697 |
| AsuC2I | CCSGG | 1 cut(s) | 2022 |
| AsuHPI | GGTGA | 3 cut(s) | 315, 1237, 2053 |
| AvaI | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| AvaII | GGWCC | 2 cut(s) | 1064, 1697 |
| BaeGI | GKGCMC | 2 cut(s) | 300, 1609 |
| BamHI | GGATCC | 2 cut(s) | 1438, 1457 |
| BanII | GRGCYC | 3 cut(s) | 472, 508, 1609 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 572, 604 |
| BauI | CACGAG | 1 cut(s) | 1387 |
| BbsI | GAAGAC | 1 cut(s) | 1143 |
| Bbv12I | GWGCWC | 1 cut(s) | 508 |
| BbvCI | CCTCAGC | 1 cut(s) | 1182 |
| BbvI | GCAGC | 2 cut(s) | 1507, 2215 |
| BccI | CCATC | 5 cut(s) | 33, 401, 1407, 1497, 1954 |
| BceAI | ACGGC | 2 cut(s) | 318, 1004 |
| BclI | TGATCA | 1 cut(s) | 688 |
| BcnI | CCSGG | 1 cut(s) | 2022 |
| BcoDI | GTCTC | 4 cut(s) | 732, 844, 1745, 1913 |
| BfaI | CTAG | 5 cut(s) | 386, 1098, 1419, 1827, 2057 |
| BfmI | CTRYAG | 3 cut(s) | 72, 1485, 1744 |
| BfuAI | ACCTGC | 2 cut(s) | 215, 1453 |
| BglII | AGATCT | 1 cut(s) | 76 |
| BisI | GCNGC | 5 cut(s) | 1296, 1496, 1499, 2052, 2229 |
| BlsI | GCNGC | 5 cut(s) | 1297, 1497, 1500, 2053, 2230 |
| Bme1390I | CCNGG | 1 cut(s) | 2022 |
| Bme18I | GGWCC | 2 cut(s) | 1064, 1697 |
| BmeT110I | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| BmgT120I | GGNCC | 4 cut(s) | 1064, 1605, 1606, 1697 |
| BmiI | GGNNCC | 6 cut(s) | 471, 817, 1440, 1459, 1607, 1698 |
| BmrFI | CCNGG | 1 cut(s) | 2022 |
| BmsI | GCATC | 7 cut(s) | 607, 634, 1076, 1231, 1722, 1854, 2211 |
| BpiI | GAAGAC | 1 cut(s) | 1143 |
| BpmI | CTGGAG | 1 cut(s) | 834 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1182, 1632 |
| BpuEI | CTTGAG | 5 cut(s) | 422, 1140, 1284, 1847, 1935 |
| BpuMI | CCSGG | 1 cut(s) | 2022 |
| Bsa29I | ATCGAT | 1 cut(s) | 1031 |
| BsaAI | YACGTR | 1 cut(s) | 592 |
| BsaI | GGTCTC | 1 cut(s) | 1913 |
| BsaJI | CCNNGG | 6 cut(s) | 300, 1060, 1348, 1564, 1692, 1768 |
| BsaWI | WCCGGW | 1 cut(s) | 1435 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 640, 1408, 1472, 1552, 1553, 1952 |
| Bse1I | ACTGG | 2 cut(s) | 817, 1335 |
| Bse3DI | GCAATG | 2 cut(s) | 930, 1244 |
| BseAI | TCCGGA | 1 cut(s) | 1435 |
| BseCI | ATCGAT | 1 cut(s) | 1031 |
| BseDI | CCNNGG | 6 cut(s) | 300, 1060, 1348, 1564, 1692, 1768 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 640, 1408, 1472, 1552, 1553, 1952 |
| BseMI | GCAATG | 2 cut(s) | 930, 1244 |
| BseMII | CTCAG | 3 cut(s) | 855, 1173, 1623 |
| BseNI | ACTGG | 2 cut(s) | 817, 1335 |
| BseRI | GAGGAG | 4 cut(s) | 98, 408, 1059, 2118 |
| BseSI | GKGCMC | 2 cut(s) | 300, 1609 |
| BseXI | GCAGC | 2 cut(s) | 1507, 2215 |
| BshFI | GGCC | 2 cut(s) | 93, 1607 |
| BshVI | ATCGAT | 1 cut(s) | 1031 |
| BsiHKAI | GWGCWC | 1 cut(s) | 508 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| BsiSI | CCGG | 3 cut(s) | 604, 1436, 2022 |
| BslFI | GGGAC | 3 cut(s) | 737, 1053, 1710 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 640, 1408, 1472, 1552, 1553, 1952 |
| BsmAI | GTCTC | 4 cut(s) | 732, 844, 1745, 1913 |
| BsmBI | CGTCTC | 1 cut(s) | 732 |
| BsmFI | GGGAC | 3 cut(s) | 737, 1053, 1710 |
| BsnI | GGCC | 2 cut(s) | 93, 1607 |
| Bso31I | GGTCTC | 1 cut(s) | 1913 |
| BsoBI | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| Bsp120I | GGGCCC | 1 cut(s) | 1605 |
| Bsp1286I | GDGCHC | 4 cut(s) | 300, 472, 508, 1609 |
| Bsp13I | TCCGGA | 1 cut(s) | 1435 |
| Bsp1407I | TGTACA | 1 cut(s) | 1709 |
| Bsp143I | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| BspACI | CCGC | 3 cut(s) | 1295, 1498, 2052 |
| BspANI | GGCC | 2 cut(s) | 93, 1607 |
| BspCNI | CTCAG | 3 cut(s) | 854, 1174, 1624 |
| BspDI | ATCGAT | 1 cut(s) | 1031 |
| BspEI | TCCGGA | 1 cut(s) | 1435 |
| BspHI | TCATGA | 2 cut(s) | 268, 1761 |
| BspLI | GGNNCC | 6 cut(s) | 471, 817, 1440, 1459, 1607, 1698 |
| BspMI | ACCTGC | 2 cut(s) | 215, 1453 |
| BspPI | GGATC | 5 cut(s) | 50, 1433, 1446, 1452, 1465 |
| BspTNI | GGTCTC | 1 cut(s) | 1913 |
| BsrBI | CCGCTC | 1 cut(s) | 2054 |
| BsrDI | GCAATG | 2 cut(s) | 930, 1244 |
| BsrGI | TGTACA | 1 cut(s) | 1709 |
| BsrI | ACTGG | 2 cut(s) | 817, 1335 |
| BssECI | CCNNGG | 6 cut(s) | 300, 1060, 1348, 1564, 1692, 1768 |
| BssMI | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| BssNAI | GTATAC | 1 cut(s) | 766 |
| BssSI | CACGAG | 1 cut(s) | 1387 |
| BssT1I | CCWWGG | 3 cut(s) | 1060, 1692, 1768 |
| Bst1107I | GTATAC | 1 cut(s) | 766 |
| Bst2BI | CACGAG | 1 cut(s) | 1387 |
| Bst4CI | ACNGT | 8 cut(s) | 313, 337, 346, 884, 1739, 1748, 2085, 2242 |
| Bst6I | CTCTTC | 2 cut(s) | 575, 1096 |
| BstAUI | TGTACA | 1 cut(s) | 1709 |
| BstBAI | YACGTR | 1 cut(s) | 592 |
| BstC8I | GCNNGC | 1 cut(s) | 1656 |
| BstDEI | CTNAG | 4 cut(s) | 450, 841, 1182, 1632 |
| BstDSI | CCRYGG | 2 cut(s) | 300, 1564 |
| BstKTI | GATC | 5 cut(s) | 45, 79, 691, 1441, 1460 |
| BstMAI | GTCTC | 4 cut(s) | 732, 844, 1745, 1913 |
| BstMBI | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1514, 2057 |
| BstNSI | RCATGY | 1 cut(s) | 892 |
| BstSCI | CCNGG | 1 cut(s) | 2020 |
| BstSFI | CTRYAG | 3 cut(s) | 72, 1485, 1744 |
| BstSLI | GKGCMC | 2 cut(s) | 300, 1609 |
| BstV1I | GCAGC | 2 cut(s) | 1507, 2215 |
| BstV2I | GAAGAC | 1 cut(s) | 1143 |
| BstX2I | RGATCY | 4 cut(s) | 42, 76, 1438, 1457 |
| BstYI | RGATCY | 4 cut(s) | 42, 76, 1438, 1457 |
| BstZ17I | GTATAC | 1 cut(s) | 766 |
| Bsu15I | ATCGAT | 1 cut(s) | 1031 |
| BsuRI | GGCC | 2 cut(s) | 93, 1607 |
| BsuTUI | ATCGAT | 1 cut(s) | 1031 |
| BtgI | CCRYGG | 2 cut(s) | 300, 1564 |
| BtgZI | GCGATG | 1 cut(s) | 1516 |
| BtsIMutI | CAGTG | 4 cut(s) | 333, 342, 1374, 1735 |
| BveI | ACCTGC | 2 cut(s) | 215, 1453 |
| Cac8I | GCNNGC | 1 cut(s) | 1656 |
| CciI | TCATGA | 2 cut(s) | 268, 1761 |
| Cfr13I | GGNCC | 4 cut(s) | 1064, 1605, 1606, 1697 |
| ClaI | ATCGAT | 1 cut(s) | 1031 |
| Csp6I | GTAC | 5 cut(s) | 589, 1327, 1360, 1710, 1781 |
| CviAII | CATG | 8 cut(s) | 269, 820, 889, 1552, 1612, 1762, 2099, 2189 |
| CviQI | GTAC | 5 cut(s) | 589, 1327, 1360, 1710, 1781 |
| DdeI | CTNAG | 4 cut(s) | 450, 841, 1182, 1632 |
| DpnI | GATC | 5 cut(s) | 44, 78, 690, 1440, 1459 |
| DpnII | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| Eam1104I | CTCTTC | 2 cut(s) | 575, 1096 |
| EarI | CTCTTC | 2 cut(s) | 575, 1096 |
| Ecl136II | GAGCTC | 1 cut(s) | 506 |
| Eco130I | CCWWGG | 3 cut(s) | 1060, 1692, 1768 |
| Eco24I | GRGCYC | 3 cut(s) | 472, 508, 1609 |
| Eco31I | GGTCTC | 1 cut(s) | 1913 |
| Eco32I | GATATC | 1 cut(s) | 1855 |
| Eco47I | GGWCC | 2 cut(s) | 1064, 1697 |
| Eco53kI | GAGCTC | 1 cut(s) | 506 |
| Eco57I | CTGAAG | 1 cut(s) | 249 |
| Eco88I | CYCGRG | 3 cut(s) | 1916, 2005, 2113 |
| EcoICRI | GAGCTC | 1 cut(s) | 506 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1606, 1697 |
| EcoRV | GATATC | 1 cut(s) | 1855 |
| EcoT14I | CCWWGG | 3 cut(s) | 1060, 1692, 1768 |
| EcoT22I | ATGCAT | 1 cut(s) | 1246 |
| EcoT38I | GRGCYC | 3 cut(s) | 472, 508, 1609 |
| ErhI | CCWWGG | 3 cut(s) | 1060, 1692, 1768 |
| Esp3I | CGTCTC | 1 cut(s) | 732 |
| FaeI | CATG | 8 cut(s) | 272, 823, 892, 1555, 1615, 1765, 2102, 2192 |
| FaqI | GGGAC | 3 cut(s) | 737, 1053, 1710 |
| FatI | CATG | 8 cut(s) | 268, 819, 888, 1551, 1611, 1761, 2098, 2188 |
| FbaI | TGATCA | 1 cut(s) | 688 |
| FblI | GTMKAC | 4 cut(s) | 765, 879, 2077, 2213 |
| Fnu4HI | GCNGC | 5 cut(s) | 1296, 1496, 1499, 2052, 2229 |
| FriOI | GRGCYC | 3 cut(s) | 472, 508, 1609 |
| Fsp4HI | GCNGC | 5 cut(s) | 1296, 1496, 1499, 2052, 2229 |
| FspBI | CTAG | 5 cut(s) | 386, 1098, 1419, 1827, 2057 |
| GluI | GCNGC | 5 cut(s) | 1296, 1496, 1499, 2052, 2229 |
| GsuI | CTGGAG | 1 cut(s) | 834 |
| HaeIII | GGCC | 2 cut(s) | 93, 1607 |
| HapII | CCGG | 3 cut(s) | 604, 1436, 2022 |
| Hin1II | CATG | 8 cut(s) | 272, 823, 892, 1555, 1615, 1765, 2102, 2192 |
| HincII | GTYRAC | 1 cut(s) | 1586 |
| HindII | GTYRAC | 1 cut(s) | 1586 |
| HindIII | AAGCTT | 3 cut(s) | 444, 663, 1865 |
| HpaII | CCGG | 3 cut(s) | 604, 1436, 2022 |
| HphI | GGTGA | 3 cut(s) | 315, 1237, 2053 |
| Hpy166II | GTNNAC | 6 cut(s) | 340, 766, 880, 1586, 2078, 2214 |
| Hpy188I | TCNGA | 2 cut(s) | 403, 1126 |
| Hpy188III | TCNNGA | 6 cut(s) | 82, 269, 774, 1301, 1436, 1762 |
| Hpy8I | GTNNAC | 6 cut(s) | 340, 766, 880, 1586, 2078, 2214 |
| Hpy99I | CGWCG | 2 cut(s) | 1471, 1513 |
| HpyAV | CCTTC | 6 cut(s) | 104, 284, 288, 484, 806, 2171 |
| HpyCH4III | ACNGT | 8 cut(s) | 313, 337, 346, 884, 1739, 1748, 2085, 2242 |
| HpyCH4IV | ACGT | 7 cut(s) | 60, 147, 492, 591, 869, 876, 1469 |
| HpyCH4V | TGCA | 8 cut(s) | 480, 539, 647, 860, 1244, 1249, 1280, 1735 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1514, 2057 |
| HpyF3I | CTNAG | 4 cut(s) | 450, 841, 1182, 1632 |
| HpySE526I | ACGT | 7 cut(s) | 60, 147, 492, 591, 869, 876, 1469 |
| Hsp92II | CATG | 8 cut(s) | 272, 823, 892, 1555, 1615, 1765, 2102, 2192 |
| Kpn2I | TCCGGA | 1 cut(s) | 1435 |
| Ksp22I | TGATCA | 1 cut(s) | 688 |
| Kzo9I | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| LmnI | GCTCC | 4 cut(s) | 475, 511, 815, 1514 |
| Lsp1109I | GCAGC | 2 cut(s) | 1507, 2215 |
| LweI | GCATC | 7 cut(s) | 607, 634, 1076, 1231, 1722, 1854, 2211 |
| MaeI | CTAG | 5 cut(s) | 386, 1098, 1419, 1827, 2057 |
| MaeII | ACGT | 7 cut(s) | 60, 147, 492, 591, 869, 876, 1469 |
| MaeIII | GTNAC | 3 cut(s) | 321, 884, 1717 |
| MalI | GATC | 5 cut(s) | 44, 78, 690, 1440, 1459 |
| MbiI | CCGCTC | 1 cut(s) | 2054 |
| MboI | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| MflI | RGATCY | 4 cut(s) | 42, 76, 1438, 1457 |
| MhlI | GDGCHC | 4 cut(s) | 300, 472, 508, 1609 |
| MlyI | GAGTC | 5 cut(s) | 229, 329, 846, 1670, 1946 |
| MmeI | TCCRAC | 4 cut(s) | 381, 621, 1334, 1375 |
| Mph1103I | ATGCAT | 1 cut(s) | 1246 |
| MroI | TCCGGA | 1 cut(s) | 1435 |
| MroXI | GAANNNNTTC | 2 cut(s) | 264, 1150 |
| MseI | TTAA | 9 cut(s) | 65, 255, 459, 524, 705, 833, 1533, 1619, 1976 |
| MslI | CAYNNNNRTG | 3 cut(s) | 190, 1556, 1724 |
| MspA1I | CMGCKG | 1 cut(s) | 1498 |
| MspI | CCGG | 3 cut(s) | 604, 1436, 2022 |
| MspR9I | CCNGG | 1 cut(s) | 2022 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1514, 2057 |
| NciI | CCSGG | 1 cut(s) | 2022 |
| NdeII | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| NlaIII | CATG | 8 cut(s) | 272, 823, 892, 1555, 1615, 1765, 2102, 2192 |
| NlaIV | GGNNCC | 6 cut(s) | 471, 817, 1440, 1459, 1607, 1698 |
| NmeAIII | GCCGAG | 1 cut(s) | 69 |
| NmuCI | GTSAC | 3 cut(s) | 321, 884, 1717 |
| NsiI | ATGCAT | 1 cut(s) | 1246 |
| NspI | RCATGY | 1 cut(s) | 892 |
| OliI | CACNNNNGTG | 1 cut(s) | 1724 |
| PagI | TCATGA | 2 cut(s) | 268, 1761 |
| PaqCI | CACCTGC | 1 cut(s) | 1453 |
| PciI | ACATGT | 1 cut(s) | 888 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1017 |
| PdmI | GAANNNNTTC | 2 cut(s) | 264, 1150 |
| PflFI | GACNNNGTC | 2 cut(s) | 1701, 1924 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 640 |
| PkrI | GCNGC | 5 cut(s) | 1297, 1497, 1500, 2053, 2230 |
| PleI | GAGTC | 5 cut(s) | 228, 328, 845, 1670, 1945 |
| PpsI | GAGTC | 5 cut(s) | 228, 328, 845, 1670, 1945 |
| Ppu21I | YACGTR | 1 cut(s) | 592 |
| PpuMI | RGGWCCY | 1 cut(s) | 1697 |
| PscI | ACATGT | 1 cut(s) | 888 |
| Psp124BI | GAGCTC | 1 cut(s) | 508 |
| Psp1406I | AACGTT | 2 cut(s) | 147, 492 |
| Psp5II | RGGWCCY | 1 cut(s) | 1697 |
| PspN4I | GGNNCC | 6 cut(s) | 471, 817, 1440, 1459, 1607, 1698 |
| PspOMI | GGGCCC | 1 cut(s) | 1605 |
| PspPI | GGNCC | 4 cut(s) | 1064, 1605, 1606, 1697 |
| PspPPI | RGGWCCY | 1 cut(s) | 1697 |
| PsuI | RGATCY | 4 cut(s) | 42, 76, 1438, 1457 |
| PsyI | GACNNNGTC | 2 cut(s) | 1701, 1924 |
| RsaI | GTAC | 5 cut(s) | 590, 1328, 1361, 1711, 1782 |
| RsaNI | GTAC | 5 cut(s) | 589, 1327, 1360, 1710, 1781 |
| RseI | CAYNNNNRTG | 3 cut(s) | 190, 1556, 1724 |
| SacI | GAGCTC | 1 cut(s) | 508 |
| SaqAI | TTAA | 9 cut(s) | 65, 255, 459, 524, 705, 833, 1533, 1619, 1976 |
| SatI | GCNGC | 5 cut(s) | 1296, 1496, 1499, 2052, 2229 |
| Sau3AI | GATC | 5 cut(s) | 42, 76, 688, 1438, 1457 |
| Sau96I | GGNCC | 4 cut(s) | 1064, 1605, 1606, 1697 |
| SchI | GAGTC | 5 cut(s) | 229, 329, 846, 1670, 1946 |
| ScrFI | CCNGG | 1 cut(s) | 2022 |
| SduI | GDGCHC | 4 cut(s) | 300, 472, 508, 1609 |
| SfaNI | GCATC | 7 cut(s) | 607, 634, 1076, 1231, 1722, 1854, 2211 |
| SfcI | CTRYAG | 3 cut(s) | 72, 1485, 1744 |
| SinI | GGWCC | 2 cut(s) | 1064, 1697 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 190, 1556, 1724 |
| SmlI | CTYRAG | 5 cut(s) | 437, 1155, 1299, 1862, 1950 |
| SmoI | CTYRAG | 5 cut(s) | 437, 1155, 1299, 1862, 1950 |
| SsiI | CCGC | 3 cut(s) | 1295, 1498, 2052 |
| SspI | AATATT | 1 cut(s) | 1150 |
| SspMI | CTAG | 5 cut(s) | 386, 1098, 1419, 1827, 2057 |
| SstI | GAGCTC | 1 cut(s) | 508 |
| StyD4I | CCNGG | 1 cut(s) | 2020 |
| StyI | CCWWGG | 3 cut(s) | 1060, 1692, 1768 |
| TaaI | ACNGT | 8 cut(s) | 313, 337, 346, 884, 1739, 1748, 2085, 2242 |
| TaiI | ACGT | 7 cut(s) | 63, 150, 495, 594, 872, 879, 1472 |
| TaqI | TCGA | 4 cut(s) | 118, 947, 1031, 1521 |
| TatI | WGTACW | 2 cut(s) | 1709, 1780 |
| TauI | GCSGC | 3 cut(s) | 1298, 1501, 2054 |
| Tru1I | TTAA | 9 cut(s) | 65, 255, 459, 524, 705, 833, 1533, 1619, 1976 |
| Tru9I | TTAA | 9 cut(s) | 65, 255, 459, 524, 705, 833, 1533, 1619, 1976 |
| TscAI | CASTG | 4 cut(s) | 340, 349, 1374, 1742 |
| TseFI | GTSAC | 3 cut(s) | 321, 884, 1717 |
| TseI | GCWGC | 2 cut(s) | 1495, 2228 |
| Tsp45I | GTSAC | 3 cut(s) | 321, 884, 1717 |
| TspGWI | ACGGA | 2 cut(s) | 1158, 1571 |
| TspRI | CASTG | 4 cut(s) | 340, 349, 1374, 1742 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1701, 1924 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 640 |
| VpaK11BI | GGWCC | 2 cut(s) | 1064, 1697 |
| XapI | RAATTY | 6 cut(s) | 462, 1210, 1577, 1978, 1988, 1994 |
| XceI | RCATGY | 1 cut(s) | 892 |
| XmiI | GTMKAC | 4 cut(s) | 765, 879, 2077, 2213 |
| XmnI | GAANNNNTTC | 2 cut(s) | 264, 1150 |
| XspI | CTAG | 5 cut(s) | 386, 1098, 1419, 1827, 2057 |
| Zsp2I | ATGCAT | 1 cut(s) | 1246 |