Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0000454

Physical Location & Seq

Genomic Coordinates Forward (+)

60355559 .. 60360084

Gene Structure 4526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0472300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGACCGCCAGGACTCCGTCACGGCCCGCTTCACTCTCGGCAGGCAGTCCTCGCTCGCCCCCGACCTGGACGACGACGACTCGGTGGTCGAGCCGGCCGACGGGGCCCTTGACCCGAGGGTCCGCCTCATGTACTTGGCCAACGAGGGCGACTTGGAAGGCATTCAGGAGCTCCTGGACTCCGGAACTAATGTCAATTTCACTGACATAGATGGACGCACCGCTCTCCACGTCGCCGCCTGTCAGGGCCGGACCGACGTCGTTCAGTTGCTCCTCCAAAGAGGCGCCGACGTCAACCTCCAGGACCGCTGGGGCAGTACGCCTCTTGCGGATGCGGTCTACTATAAGAATGAGGATGTGATCAAGCTTTTAGAGCAGCACGGAGCTAAACTTCCGATGGCTCCTATGCATGTGGAGAATGCACGTGAAGTCCCGGAGTATGAGATTGATCCCAAGGAGCTTGATTTTTCAGATAGTGTTGACATAACCAAGGGAACCTACTGCATTGCATCTTGGCGTGGAATTCAAGTTGCAGTTAAGACGCTTGAGGAGAAAGTATTTGCTGATGAAGATAAAGTAAATGCATTCAGGGATGAGCTTGCTTTACTTCAGAAGATACGACATCCCAATGTTGTCCAATTTTTGGGTGCTGTAACACAAAGCAGTCCAATGATGATTGTCACAGAGTATCTACCCAAGGGAGATTTTCGTTTATATCTTAAAAGAAAAGGTCCCTTAAAGCCAACAGCAGCAGTGAAGTTTGCACTTGACATTGCCAGGGGGATGAATTATCTGCATGAGCATAAACCTGAAGCAATAATTCATCGAGATCTTGAGCCCTCAAATATATTGCGGGATGATTCTGGGCATCTGAAAGTTGCAGACTTTGGGGTGAGCAAGCTACTGATAGTCGCAAAGACAGTTAAAGAAGACAGACCTGTGACTACTGAAGACACTTCTTGGAGATACGTGGCTTCTGAAGTTTACAGAAATGAAGAATATGATACCAAAGTGGATGTTTTTTCATTTGCCTTGATCTTGCAAGAGATGATTGAAGGTTGTCTACCATTTTCTACAAAACCAGAAAAAGAAGTACCTAAAGCATATGCTGCAAATGAGCGCCCACCTTTTAGAGCACCACCAAAGCTTTATGCACATGGATTAAAGGAGCATGGTGTTGACCTTTCCTGTCAAGAAGATTATATTGGTGATGCCACTTATGTGTCTCCAGAACCTGGAGAACATGGTGAAGAAGGGTCGCTCAAGTCCCAGCAGCCATTCATCCTGCTCTACGATGAGATGAGTAACTATTGCCTTCATGCAGGATTTCAGCTGTTTTTCCTCCTCGATTTGAGGAAGGTTTTTCTAGCGGAGTTTATAGATTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009653 GO:0009832 GO:0009833 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016049 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0021700 GO:0022402 GO:0022622 GO:0030154 GO:0030243 GO:0030244 GO:0032501 GO:0032502 GO:0033692 GO:0034637 GO:0034645 GO:0040007 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0051273 GO:0051274 GO:0051301 GO:0060560 GO:0061640 GO:0071554 GO:0071669 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:1901576 GO:1903047 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt

Protein Analysis

462

Amino Acids

52.04

Weight (kDa)

4.86

Isoelectric Point (pI)

38.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	43 - 130	1.1e-18	Ankyrin repeats (3 copies)
Ank_4	PF13637	43 - 92	1.9e-08	Ankyrin repeats (many copies)
Ank_5	PF13857	64 - 109	7.9e-09	Ankyrin repeats (many copies)
Ank	PF00023	71 - 103	8.2e-11	Ankyrin repeat
Ank_4	PF13637	82 - 124	1.7e-06	Ankyrin repeats (many copies)
PK_Tyr_Ser-Thr	PF07714	162 - 377	6.3e-45	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	168 - 364	3.9e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32530 AT2G32540 AT2G32620 AT2G32620 AT2G32620 AT2G32620 AT4G15290 AT4G15320 AT4G15320 AT4G15320 AT4G15320 AT4G15320
fragaria_vesca	FvH4_6g42780 FvH4_6g42780 FvH4_6g42780 FvH4_6g42780 FvH4_6g42780 FvH4_6g42780 FvH4_6g42780 FvH4_6g42800 FvH4_6g42840 FvH4_6g42840
malus_domestica	MD09G1072200.v1.1 MD17G1099500.v1.1 MD17G1099600.v1.1 MD17G1099800.v1.1
prunus_persica	Prupe.3G214200_v2.0.a1 Prupe.3G214200_v2.0.a1 Prupe.3G214200_v2.0.a1 Prupe.3G214200_v2.0.a1 Prupe.3G214200_v2.0.a1 Prupe.3G214200_v2.0.a1 Prupe.3G214400_v2.0.a1 Prupe.3G214400_v2.0.a1
pyrus_communis	pycom09g03400 pycom17g09490 pycom17g09510
rosa_chinensis	RchiOBHm_Chr2g0158721 RchiOBHm_Chr2g0158731 RchiOBHm_Chr2g0158741 RchiOBHm_Chr2g0158761 RchiOBHm_Chr2g0158771
rosa_laevigata	RLG00000021109 RLG00000021110 RLG00000021111
rosa_multiflora	Rmu_co8470225.1_g000001 Rmu_sc0003664.1_g000005 Rmu_sc0003664.1_g000006 Rmu_sc0003664.1_g000007 Rmu_sc0003664.1_g000012 Rmu_sc0004188.1_g000001 Rmu_sc0004188.1_g000006 Rmu_sc0004188.1_g000008 Rmu_sc0024429.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091020 Rroxscaffold_2G00091030 Rroxscaffold_2G00091040 Rroxscaffold_2G00091050 Rroxscaffold_2G00091060 Rroxscaffold_2G00091080
rosa_rugosa	Rorug02G0471500 Rorug02G0471600 Rorug02G0471800 Rorug02G0471900 Rorug02G0471900 Rorug02G0472200 Rorug02G0472300 Rorug07G0260700
rosa_samantha	Rh1BG072200 Rh2AG537300 Rh2AG537400 Rh2AG537500 Rh2AG538000 Rh2AG538100 Rh2AG538200 Rh2BG550500 Rh2BG550600 Rh2BG550800 Rh2CG521000 Rh2CG521100 Rh2CG521200 Rh2CG521300 Rh2DG560200 Rh2DG560300 Rh2DG560400 Rh2DG560500 Rh2DG560600
rosa_wichuraiana	Rw2G044490 Rw2G044500 Rw2G044510 Rw2G044520 Rw2G044530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	86, 119
AatII	GACGTC	2 cut(s)	261, 294
AccB1I	GGYRCC	1 cut(s)	284
AccB7I	CCANNNNNTGG	2 cut(s)	643, 1235
AccBSI	CCGCTC	1 cut(s)	224
AccI	GTMKAC	2 cut(s)	339, 1063
AccIII	TCCGGA	1 cut(s)	182
AclWI	GGATC	1 cut(s)	443
AcoI	YGGCCR	2 cut(s)	96, 138
AcsI	RAATTY	1 cut(s)	522
AcuI	CTGAAG	4 cut(s)	593, 831, 969, 999
AcvI	CACGTG	1 cut(s)	425
AcyI	GRCGYC	3 cut(s)	258, 285, 291
AfaI	GTAC	3 cut(s)	134, 319, 1095
AfiI	CCNNNNNNNGG	4 cut(s)	67, 101, 643, 1235
AgsI	TTSAA	2 cut(s)	527, 1055
AjiI	CACGTC	1 cut(s)	232
AjnI	CCWGG	6 cut(s)	9, 66, 174, 300, 776, 1234
AjuI	GAANNNNNNNTTGG	2 cut(s)	1188, 1220
AleI	CACNNNNGTG	1 cut(s)	1220
AluBI	AGCT	8 cut(s)	172, 367, 386, 460, 598, 901, 1147, 1333
AluI	AGCT	8 cut(s)	172, 367, 386, 460, 598, 901, 1147, 1333
Alw21I	GWGCWC	2 cut(s)	174, 1138
Alw26I	GTCTC	1 cut(s)	1230
AlwI	GGATC	1 cut(s)	443
AlwNI	CAGNNNCTG	1 cut(s)	1235
Ama87I	CYCGRG	1 cut(s)	115
Aor13HI	TCCGGA	1 cut(s)	182
AoxI	GGCC	5 cut(s)	24, 96, 105, 138, 247
ApaI	GGGCCC	1 cut(s)	109
ApeKI	GCWGC	4 cut(s)	376, 749, 1109, 1273
ApoI	RAATTY	1 cut(s)	522
Asp700I	GAANNNNTTC	1 cut(s)	162
AspLEI	GCGC	2 cut(s)	287, 1122
AspS9I	GGNCC	9 cut(s)	4, 25, 105, 106, 121, 247, 252, 304, 731
AsuC2I	CCSGG	1 cut(s)	434
AsuHPI	GGTGA	3 cut(s)	904, 1220, 1259
AvaI	CYCGRG	1 cut(s)	115
AvaII	GGWCC	5 cut(s)	4, 121, 252, 304, 731
BaeGI	GKGCMC	1 cut(s)	109
BaeI	ACNNNNGTAYC	2 cut(s)	996, 1029
BalI	TGGCCA	1 cut(s)	140
BanI	GGYRCC	1 cut(s)	284
BanII	GRGCYC	3 cut(s)	109, 174, 840
BarI	GAAGNNNNNNTAC	2 cut(s)	1047, 1079
BbrPI	CACGTG	1 cut(s)	425
BbsI	GAAGAC	2 cut(s)	936, 957
Bbv12I	GWGCWC	2 cut(s)	174, 1138
BbvI	GCAGC	4 cut(s)	388, 761, 1096, 1285
BccI	CCATC	2 cut(s)	206, 391
BceAI	ACGGC	1 cut(s)	39
BciT130I	CCWGG	6 cut(s)	11, 68, 176, 302, 778, 1236
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	1 cut(s)	434
BcoDI	GTCTC	1 cut(s)	1230
BfaI	CTAG	1 cut(s)	1367
BfoI	RGCGCY	2 cut(s)	288, 1123
BglI	GCCNNNNNGGC	1 cut(s)	104
BglII	AGATCT	1 cut(s)	829
BisI	GCNGC	5 cut(s)	237, 377, 750, 1110, 1274
BlsI	GCNGC	5 cut(s)	238, 378, 751, 1111, 1275
Bme1390I	CCNGG	7 cut(s)	11, 68, 176, 302, 434, 778, 1236
Bme18I	GGWCC	5 cut(s)	4, 121, 252, 304, 731
BmeT110I	CYCGRG	1 cut(s)	115
BmgBI	CACGTC	1 cut(s)	232
BmgT120I	GGNCC	9 cut(s)	4, 25, 105, 106, 121, 247, 252, 304, 731
BmiI	GGNNCC	7 cut(s)	106, 107, 122, 286, 402, 496, 733
BmrFI	CCNGG	7 cut(s)	11, 68, 176, 302, 434, 778, 1236
BmsI	GCATC	4 cut(s)	322, 518, 877, 1201
BoxI	GACNNNNGTC	1 cut(s)	257
BpiI	GAAGAC	2 cut(s)	936, 957
BpmI	CTGGAG	3 cut(s)	284, 1212, 1257
BpuEI	CTTGAG	3 cut(s)	566, 854, 1247
BpuMI	CCSGG	1 cut(s)	434
BsaAI	YACGTR	2 cut(s)	425, 970
BsaHI	GRCGYC	3 cut(s)	258, 285, 291
BsaJI	CCNNGG	5 cut(s)	116, 453, 489, 696, 777
BsaWI	WCCGGW	1 cut(s)	182
Bsc4I	CCNNNNNNNGG	4 cut(s)	67, 101, 643, 1235
Bse118I	RCCGGY	1 cut(s)	94
Bse3DI	GCAATG	2 cut(s)	504, 771
BseAI	TCCGGA	1 cut(s)	182
BseBI	CCWGG	6 cut(s)	11, 68, 176, 302, 778, 1236
BseDI	CCNNGG	5 cut(s)	116, 453, 489, 696, 777
BseGI	GGATG	8 cut(s)	337, 361, 598, 622, 789, 862, 1021, 1281
BseLI	CCNNNNNNNGG	4 cut(s)	67, 101, 643, 1235
BseMI	GCAATG	2 cut(s)	504, 771
BseRI	GAGGAG	3 cut(s)	263, 563, 1334
BseSI	GKGCMC	1 cut(s)	109
BseX3I	CGGCCG	1 cut(s)	96
BseXI	GCAGC	4 cut(s)	388, 761, 1096, 1285
BseYI	CCCAGC	2 cut(s)	309, 1269
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	5 cut(s)	26, 98, 107, 140, 249
BshNI	GGYRCC	1 cut(s)	284
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	2 cut(s)	174, 1138
BsiHKCI	CYCGRG	1 cut(s)	115
BsiSI	CCGG	4 cut(s)	95, 183, 250, 434
BslFI	GGGAC	3 cut(s)	416, 717, 1252
BslI	CCNNNNNNNGG	4 cut(s)	67, 101, 643, 1235
BsmAI	GTCTC	1 cut(s)	1230
BsmFI	GGGAC	3 cut(s)	416, 717, 1252
BsmI	GAATGC	3 cut(s)	162, 424, 584
BsnI	GGCC	5 cut(s)	26, 98, 107, 140, 249
BsoBI	CYCGRG	1 cut(s)	115
Bsp120I	GGGCCC	1 cut(s)	105
Bsp1286I	GDGCHC	4 cut(s)	109, 174, 840, 1138
Bsp13I	TCCGGA	1 cut(s)	182
Bsp143I	GATC	4 cut(s)	360, 448, 829, 1035
BspANI	GGCC	5 cut(s)	26, 98, 107, 140, 249
BspEI	TCCGGA	1 cut(s)	182
BspLI	GGNNCC	7 cut(s)	106, 107, 122, 286, 402, 496, 733
BspPI	GGATC	1 cut(s)	443
BspT107I	GGYRCC	1 cut(s)	284
BsrBI	CCGCTC	1 cut(s)	224
BsrDI	GCAATG	2 cut(s)	504, 771
BsrFI	RCCGGY	1 cut(s)	94
BssAI	RCCGGY	1 cut(s)	94
BssECI	CCNNGG	5 cut(s)	116, 453, 489, 696, 777
BssMI	GATC	4 cut(s)	360, 448, 829, 1035
BssNI	GRCGYC	3 cut(s)	258, 285, 291
BssT1I	CCWWGG	3 cut(s)	453, 489, 696
Bst2UI	CCWGG	6 cut(s)	11, 68, 176, 302, 778, 1236
Bst4CI	ACNGT	1 cut(s)	922
BstACI	GRCGYC	3 cut(s)	258, 285, 291
BstAPI	GCANNNNNTGC	1 cut(s)	1109
BstBAI	YACGTR	2 cut(s)	425, 970
BstC8I	GCNNGC	6 cut(s)	28, 44, 57, 96, 600, 899
BstF5I	GGATG	8 cut(s)	337, 361, 598, 622, 789, 862, 1021, 1281
BstH2I	RGCGCY	2 cut(s)	288, 1123
BstHHI	GCGC	2 cut(s)	287, 1122
BstKTI	GATC	4 cut(s)	363, 451, 832, 1038
BstMAI	GTCTC	1 cut(s)	1230
BstMBI	GATC	4 cut(s)	360, 448, 829, 1035
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	4 cut(s)	52, 104, 373, 1109
BstNI	CCWGG	6 cut(s)	11, 68, 176, 302, 778, 1236
BstNSI	RCATGY	1 cut(s)	413
BstPAI	GACNNNNGTC	1 cut(s)	257
BstSCI	CCNGG	7 cut(s)	9, 66, 174, 300, 432, 776, 1234
BstSLI	GKGCMC	1 cut(s)	109
BstV1I	GCAGC	4 cut(s)	388, 761, 1096, 1285
BstV2I	GAAGAC	2 cut(s)	936, 957
BstX2I	RGATCY	1 cut(s)	829
BstYI	RGATCY	1 cut(s)	829
BstZI	CGGCCG	1 cut(s)	96
BsuRI	GGCC	5 cut(s)	26, 98, 107, 140, 249
BtrI	CACGTC	1 cut(s)	232
BtsCI	GGATG	8 cut(s)	337, 361, 598, 622, 789, 862, 1021, 1281
BtsI	GCAGTG	1 cut(s)	759
BtsIMutI	CAGTG	2 cut(s)	201, 759
Cac8I	GCNNGC	6 cut(s)	28, 44, 57, 96, 600, 899
CaiI	CAGNNNCTG	1 cut(s)	1235
CfoI	GCGC	2 cut(s)	287, 1122
Cfr10I	RCCGGY	1 cut(s)	94
Cfr13I	GGNCC	9 cut(s)	4, 25, 105, 106, 121, 247, 252, 304, 731
CpoI	CGGWCCG	1 cut(s)	252
CseI	GACGC	2 cut(s)	225, 550
Csp6I	GTAC	3 cut(s)	133, 318, 1094
CspI	CGGWCCG	1 cut(s)	252
CviAII	CATG	7 cut(s)	130, 410, 797, 1157, 1172, 1244, 1319
CviQI	GTAC	3 cut(s)	133, 318, 1094
DinI	GGCGCC	1 cut(s)	286
DpnI	GATC	4 cut(s)	362, 450, 831, 1037
DpnII	GATC	4 cut(s)	360, 448, 829, 1035
DrdI	GACNNNNNNGTC	2 cut(s)	86, 119
DseDI	GACNNNNNNGTC	2 cut(s)	86, 119
EaeI	YGGCCR	2 cut(s)	96, 138
EagI	CGGCCG	1 cut(s)	96
EciI	GGCGGA	1 cut(s)	113
Ecl136II	GAGCTC	1 cut(s)	172
EclXI	CGGCCG	1 cut(s)	96
Eco130I	CCWWGG	3 cut(s)	453, 489, 696
Eco24I	GRGCYC	3 cut(s)	109, 174, 840
Eco47I	GGWCC	5 cut(s)	4, 121, 252, 304, 731
Eco52I	CGGCCG	1 cut(s)	96
Eco53kI	GAGCTC	1 cut(s)	172
Eco57I	CTGAAG	4 cut(s)	593, 831, 969, 999
Eco72I	CACGTG	1 cut(s)	425
Eco88I	CYCGRG	1 cut(s)	115
EcoICRI	GAGCTC	1 cut(s)	172
EcoO109I	RGGNCCY	3 cut(s)	105, 106, 731
EcoRI	GAATTC	1 cut(s)	522
EcoRII	CCWGG	6 cut(s)	9, 66, 174, 300, 776, 1234
EcoT14I	CCWWGG	3 cut(s)	453, 489, 696
EcoT22I	ATGCAT	2 cut(s)	411, 586
EcoT38I	GRGCYC	3 cut(s)	109, 174, 840
EgeI	GGCGCC	1 cut(s)	286
EheI	GGCGCC	1 cut(s)	286
ErhI	CCWWGG	3 cut(s)	453, 489, 696
FaeI	CATG	7 cut(s)	133, 413, 800, 1160, 1175, 1247, 1322
FaqI	GGGAC	3 cut(s)	416, 717, 1252
FatI	CATG	7 cut(s)	129, 409, 796, 1156, 1171, 1243, 1318
FauI	CCCGC	2 cut(s)	35, 846
FauNDI	CATATG	1 cut(s)	1105
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	2 cut(s)	339, 1063
Fnu4HI	GCNGC	5 cut(s)	237, 377, 750, 1110, 1274
FokI	GGATG	8 cut(s)	344, 368, 605, 609, 796, 869, 1028, 1268
FriOI	GRGCYC	3 cut(s)	109, 174, 840
Fsp4HI	GCNGC	5 cut(s)	237, 377, 750, 1110, 1274
FspBI	CTAG	1 cut(s)	1367
GlaI	GCGC	2 cut(s)	286, 1121
GluI	GCNGC	5 cut(s)	237, 377, 750, 1110, 1274
GsaI	CCCAGC	2 cut(s)	313, 1273
GsuI	CTGGAG	3 cut(s)	284, 1212, 1257
HaeII	RGCGCY	2 cut(s)	288, 1123
HaeIII	GGCC	5 cut(s)	26, 98, 107, 140, 249
HapII	CCGG	4 cut(s)	95, 183, 250, 434
HgaI	GACGC	2 cut(s)	225, 550
HhaI	GCGC	2 cut(s)	287, 1122
Hin1I	GRCGYC	3 cut(s)	258, 285, 291
Hin1II	CATG	7 cut(s)	133, 413, 800, 1160, 1175, 1247, 1322
Hin6I	GCGC	2 cut(s)	285, 1120
HinP1I	GCGC	2 cut(s)	285, 1120
HincII	GTYRAC	3 cut(s)	295, 481, 1180
HindII	GTYRAC	3 cut(s)	295, 481, 1180
HindIII	AAGCTT	2 cut(s)	365, 1145
HinfI	GANTC	4 cut(s)	14, 80, 179, 860
HpaII	CCGG	4 cut(s)	95, 183, 250, 434
HphI	GGTGA	3 cut(s)	904, 1220, 1259
Hpy166II	GTNNAC	6 cut(s)	295, 340, 481, 985, 1064, 1180
Hpy188I	TCNGA	5 cut(s)	396, 472, 612, 873, 979
Hpy188III	TCNNGA	6 cut(s)	167, 183, 827, 833, 1193, 1229
Hpy8I	GTNNAC	6 cut(s)	295, 340, 481, 985, 1064, 1180
Hpy99I	CGWCG	7 cut(s)	77, 80, 104, 236, 260, 263, 293
HpyAV	CCTTC	5 cut(s)	152, 1049, 1247, 1325, 1351
HpyCH4III	ACNGT	1 cut(s)	922
HpyCH4IV	ACGT	5 cut(s)	231, 258, 291, 424, 969
HpyF10VI	GCNNNNNNNGC	4 cut(s)	52, 104, 373, 1109
HpySE526I	ACGT	5 cut(s)	231, 258, 291, 424, 969
Hsp92I	GRCGYC	3 cut(s)	258, 285, 291
Hsp92II	CATG	7 cut(s)	133, 413, 800, 1160, 1175, 1247, 1322
HspAI	GCGC	2 cut(s)	285, 1120
KasI	GGCGCC	1 cut(s)	284
Kpn2I	TCCGGA	1 cut(s)	182
KroI	GCCGGC	1 cut(s)	94
KroNI	GCCGGC	1 cut(s)	96
Ksp22I	TGATCA	1 cut(s)	360
Kzo9I	GATC	4 cut(s)	360, 448, 829, 1035
LmnI	GCTCC	7 cut(s)	169, 177, 276, 383, 406, 457, 1168
Lsp1109I	GCAGC	4 cut(s)	388, 761, 1096, 1285
LweI	GCATC	4 cut(s)	322, 518, 877, 1201
MaeI	CTAG	1 cut(s)	1367
MaeII	ACGT	5 cut(s)	231, 258, 291, 424, 969
MaeIII	GTNAC	5 cut(s)	19, 652, 679, 940, 1304
MalI	GATC	4 cut(s)	362, 450, 831, 1037
MbiI	CCGCTC	1 cut(s)	224
MboI	GATC	4 cut(s)	360, 448, 829, 1035
MboII	GAAGA	7 cut(s)	581, 625, 941, 962, 1007, 1208, 1262
MflI	RGATCY	1 cut(s)	829
MhlI	GDGCHC	4 cut(s)	109, 174, 840, 1138
MlsI	TGGCCA	1 cut(s)	140
MluCI	AATT	5 cut(s)	196, 522, 638, 787, 819
MluNI	TGGCCA	1 cut(s)	140
Mly113I	GGCGCC	1 cut(s)	285
MlyI	GAGTC	3 cut(s)	8, 74, 173
Mox20I	TGGCCA	1 cut(s)	140
Mph1103I	ATGCAT	2 cut(s)	411, 586
MroI	TCCGGA	1 cut(s)	182
MroNI	GCCGGC	1 cut(s)	94
MroXI	GAANNNNTTC	1 cut(s)	162
MscI	TGGCCA	1 cut(s)	140
MseI	TTAA	5 cut(s)	537, 720, 737, 924, 1163
MslI	CAYNNNNRTG	2 cut(s)	627, 1220
Msp20I	TGGCCA	1 cut(s)	140
MspA1I	CMGCKG	2 cut(s)	309, 1333
MspI	CCGG	4 cut(s)	95, 183, 250, 434
MspR9I	CCNGG	7 cut(s)	11, 68, 176, 302, 434, 778, 1236
Mva1269I	GAATGC	3 cut(s)	162, 424, 584
MvaI	CCWGG	6 cut(s)	11, 68, 176, 302, 778, 1236
MwoI	GCNNNNNNNGC	4 cut(s)	52, 104, 373, 1109
NaeI	GCCGGC	1 cut(s)	96
NarI	GGCGCC	1 cut(s)	285
NciI	CCSGG	1 cut(s)	434
NdeI	CATATG	1 cut(s)	1105
NdeII	GATC	4 cut(s)	360, 448, 829, 1035
NgoMIV	GCCGGC	1 cut(s)	94
NlaIII	CATG	7 cut(s)	133, 413, 800, 1160, 1175, 1247, 1322
NlaIV	GGNNCC	7 cut(s)	106, 107, 122, 286, 402, 496, 733
NmeAIII	GCCGAG	1 cut(s)	18
NmuCI	GTSAC	3 cut(s)	19, 679, 940
NsiI	ATGCAT	2 cut(s)	411, 586
NspI	RCATGY	1 cut(s)	413
OliI	CACNNNNGTG	1 cut(s)	1220
PcsI	WCGNNNNNNNCGW	1 cut(s)	96
PctI	GAATGC	3 cut(s)	162, 424, 584
PdiI	GCCGGC	1 cut(s)	96
PdmI	GAANNNNTTC	1 cut(s)	162
PfeI	GAWTC	1 cut(s)	860
PflFI	GACNNNGTC	1 cut(s)	17
PflMI	CCANNNNNTGG	2 cut(s)	643, 1235
PfoI	TCCNGGA	3 cut(s)	174, 300, 432
PkrI	GCNGC	5 cut(s)	238, 378, 751, 1111, 1275
PleI	GAGTC	3 cut(s)	8, 74, 173
PluTI	GGCGCC	1 cut(s)	288
PmaCI	CACGTG	1 cut(s)	425
PmlI	CACGTG	1 cut(s)	425
PpsI	GAGTC	3 cut(s)	8, 74, 173
Ppu21I	YACGTR	2 cut(s)	425, 970
PpuMI	RGGWCCY	1 cut(s)	731
PshAI	GACNNNNGTC	1 cut(s)	257
Psp124BI	GAGCTC	1 cut(s)	174
Psp5II	RGGWCCY	1 cut(s)	731
Psp6I	CCWGG	6 cut(s)	9, 66, 174, 300, 776, 1234
PspCI	CACGTG	1 cut(s)	425
PspFI	CCCAGC	2 cut(s)	309, 1269
PspGI	CCWGG	6 cut(s)	9, 66, 174, 300, 776, 1234
PspN4I	GGNNCC	7 cut(s)	106, 107, 122, 286, 402, 496, 733
PspOMI	GGGCCC	1 cut(s)	105
PspPI	GGNCC	9 cut(s)	4, 25, 105, 106, 121, 247, 252, 304, 731
PspPPI	RGGWCCY	1 cut(s)	731
PstNI	CAGNNNCTG	1 cut(s)	1235
PsuI	RGATCY	1 cut(s)	829
PsyI	GACNNNGTC	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	1333
RsaI	GTAC	3 cut(s)	134, 319, 1095
RsaNI	GTAC	3 cut(s)	133, 318, 1094
RseI	CAYNNNNRTG	2 cut(s)	627, 1220
Rsr2I	CGGWCCG	1 cut(s)	252
RsrII	CGGWCCG	1 cut(s)	252
SacI	GAGCTC	1 cut(s)	174
SaqAI	TTAA	5 cut(s)	537, 720, 737, 924, 1163
SatI	GCNGC	5 cut(s)	237, 377, 750, 1110, 1274
Sau3AI	GATC	4 cut(s)	360, 448, 829, 1035
Sau96I	GGNCC	9 cut(s)	4, 25, 105, 106, 121, 247, 252, 304, 731
SchI	GAGTC	3 cut(s)	8, 74, 173
ScrFI	CCNGG	7 cut(s)	11, 68, 176, 302, 434, 778, 1236
SduI	GDGCHC	4 cut(s)	109, 174, 840, 1138
SfaNI	GCATC	4 cut(s)	322, 518, 877, 1201
SfiI	GGCCNNNNNGGCC	1 cut(s)	104
SfoI	GGCGCC	1 cut(s)	286
SinI	GGWCC	5 cut(s)	4, 121, 252, 304, 731
SmiMI	CAYNNNNRTG	2 cut(s)	627, 1220
SmlI	CTYRAG	3 cut(s)	545, 833, 1262
SmoI	CTYRAG	3 cut(s)	545, 833, 1262
Sse9I	AATT	5 cut(s)	196, 522, 638, 787, 819
SspDI	GGCGCC	1 cut(s)	284
SspMI	CTAG	1 cut(s)	1367
SstI	GAGCTC	1 cut(s)	174
StyD4I	CCNGG	7 cut(s)	9, 66, 174, 300, 432, 776, 1234
StyI	CCWWGG	3 cut(s)	453, 489, 696
TaaI	ACNGT	1 cut(s)	922
TaiI	ACGT	5 cut(s)	234, 261, 294, 427, 972
TaqI	TCGA	3 cut(s)	90, 826, 1347
TaqII	GACCGA	1 cut(s)	269
TasI	AATT	5 cut(s)	196, 522, 638, 787, 819
TatI	WGTACW	1 cut(s)	132
TauI	GCSGC	1 cut(s)	239
TfiI	GAWTC	1 cut(s)	860
Tru1I	TTAA	5 cut(s)	537, 720, 737, 924, 1163
Tru9I	TTAA	5 cut(s)	537, 720, 737, 924, 1163
TscAI	CASTG	2 cut(s)	208, 759
TseFI	GTSAC	3 cut(s)	19, 679, 940
TseI	GCWGC	4 cut(s)	376, 749, 1109, 1273
Tsp45I	GTSAC	3 cut(s)	19, 679, 940
TspDTI	ATGAA	7 cut(s)	582, 800, 812, 1008, 1014, 1270, 1307
TspGWI	ACGGA	2 cut(s)	7, 396
TspRI	CASTG	2 cut(s)	208, 759
Tth111I	GACNNNGTC	1 cut(s)	17
Van91I	CCANNNNNTGG	2 cut(s)	643, 1235
VpaK11BI	GGWCC	5 cut(s)	4, 121, 252, 304, 731
XapI	RAATTY	1 cut(s)	522
XceI	RCATGY	1 cut(s)	413
XmiI	GTMKAC	2 cut(s)	339, 1063
XmnI	GAANNNNTTC	1 cut(s)	162
XspI	CTAG	1 cut(s)	1367
ZraI	GACGTC	2 cut(s)	259, 292
Zsp2I	ATGCAT	2 cut(s)	411, 586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.