Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 95 - 399 | 1.8e-63 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 930 |
| AccI | GTMKAC | 1 cut(s) | 255 |
| AccIII | TCCGGA | 1 cut(s) | 859 |
| AciI | CCGC | 4 cut(s) | 71, 801, 891, 930 |
| AclWI | GGATC | 2 cut(s) | 1077, 1109 |
| AdeI | CACNNNGTG | 2 cut(s) | 494, 1467 |
| AfaI | GTAC | 5 cut(s) | 7, 69, 905, 1109, 1171 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1269 |
| AflIII | ACRYGT | 1 cut(s) | 739 |
| AgsI | TTSAA | 1 cut(s) | 530 |
| AjiI | CACGTC | 2 cut(s) | 245, 617 |
| AjnI | CCWGG | 3 cut(s) | 91, 310, 876 |
| AluBI | AGCT | 4 cut(s) | 134, 725, 784, 1136 |
| AluI | AGCT | 4 cut(s) | 134, 725, 784, 1136 |
| Alw21I | GWGCWC | 1 cut(s) | 1197 |
| Alw26I | GTCTC | 1 cut(s) | 430 |
| Alw44I | GTGCAC | 1 cut(s) | 1193 |
| AlwI | GGATC | 2 cut(s) | 1077, 1109 |
| Aor13HI | TCCGGA | 1 cut(s) | 859 |
| AoxI | GGCC | 5 cut(s) | 239, 313, 619, 1042, 1357 |
| ApaI | GGGCCC | 1 cut(s) | 1361 |
| ApaLI | GTGCAC | 1 cut(s) | 1193 |
| ApeKI | GCWGC | 1 cut(s) | 734 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 671, 703 |
| AseI | ATTAAT | 1 cut(s) | 729 |
| AspS9I | GGNCC | 5 cut(s) | 209, 239, 483, 1357, 1358 |
| AsuC2I | CCSGG | 1 cut(s) | 238 |
| AsuHPI | GGTGA | 5 cut(s) | 858, 1105, 1309, 1441, 1453 |
| AsuII | TTCGAA | 1 cut(s) | 261 |
| AvaII | GGWCC | 2 cut(s) | 209, 483 |
| BaeGI | GKGCMC | 2 cut(s) | 1197, 1361 |
| BanII | GRGCYC | 1 cut(s) | 1361 |
| BauI | CACGAG | 1 cut(s) | 489 |
| BbsI | GAAGAC | 3 cut(s) | 89, 619, 1252 |
| Bbv12I | GWGCWC | 1 cut(s) | 1197 |
| BbvI | GCAGC | 1 cut(s) | 746 |
| BccI | CCATC | 5 cut(s) | 200, 383, 563, 1084, 1406 |
| BciT130I | CCWGG | 3 cut(s) | 93, 312, 878 |
| BciVI | GTATCC | 1 cut(s) | 1205 |
| BclI | TGATCA | 1 cut(s) | 1299 |
| BcnI | CCSGG | 1 cut(s) | 238 |
| BcoDI | GTCTC | 1 cut(s) | 430 |
| BfaI | CTAG | 4 cut(s) | 59, 827, 1143, 1235 |
| BfmI | CTRYAG | 2 cut(s) | 705, 796 |
| BfuI | GTATCC | 1 cut(s) | 1205 |
| BglII | AGATCT | 1 cut(s) | 970 |
| BisI | GCNGC | 1 cut(s) | 735 |
| BlsI | GCNGC | 1 cut(s) | 736 |
| BmcAI | AGTACT | 1 cut(s) | 7 |
| Bme1390I | CCNGG | 4 cut(s) | 93, 238, 312, 878 |
| Bme18I | GGWCC | 2 cut(s) | 209, 483 |
| BmgBI | CACGTC | 2 cut(s) | 245, 617 |
| BmgT120I | GGNCC | 5 cut(s) | 209, 239, 483, 1357, 1358 |
| BmiI | GGNNCC | 3 cut(s) | 484, 1359, 1360 |
| BmrFI | CCNGG | 4 cut(s) | 93, 238, 312, 878 |
| BmrI | ACTGGG | 1 cut(s) | 389 |
| BmsI | GCATC | 6 cut(s) | 82, 154, 596, 1000, 1022, 1071 |
| BmuI | ACTGGG | 1 cut(s) | 389 |
| BpiI | GAAGAC | 3 cut(s) | 89, 619, 1252 |
| BpmI | CTGGAG | 1 cut(s) | 1428 |
| Bpu14I | TTCGAA | 1 cut(s) | 261 |
| BpuEI | CTTGAG | 2 cut(s) | 905, 1088 |
| BpuMI | CCSGG | 1 cut(s) | 238 |
| Bsa29I | ATCGAT | 1 cut(s) | 657 |
| BsaJI | CCNNGG | 3 cut(s) | 212, 429, 478 |
| BsaWI | WCCGGW | 1 cut(s) | 859 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 576, 606 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1269 |
| Bse1I | ACTGG | 3 cut(s) | 384, 1222, 1445 |
| Bse3DI | GCAATG | 4 cut(s) | 116, 1153, 1209, 1429 |
| BseAI | TCCGGA | 1 cut(s) | 859 |
| BseBI | CCWGG | 3 cut(s) | 93, 312, 878 |
| BseCI | ATCGAT | 1 cut(s) | 657 |
| BseDI | CCNNGG | 3 cut(s) | 212, 429, 478 |
| BseGI | GGATG | 3 cut(s) | 211, 1317, 1441 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1269 |
| BseMI | GCAATG | 4 cut(s) | 116, 1153, 1209, 1429 |
| BseMII | CTCAG | 2 cut(s) | 153, 1196 |
| BseNI | ACTGG | 3 cut(s) | 384, 1222, 1445 |
| BseRI | GAGGAG | 2 cut(s) | 579, 597 |
| BseSI | GKGCMC | 2 cut(s) | 1197, 1361 |
| BseXI | GCAGC | 1 cut(s) | 746 |
| BsgI | GTGCAG | 2 cut(s) | 981, 1209 |
| BshFI | GGCC | 5 cut(s) | 241, 315, 621, 1044, 1359 |
| BshVI | ATCGAT | 1 cut(s) | 657 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1197 |
| BsiSI | CCGG | 2 cut(s) | 237, 860 |
| BslFI | GGGAC | 2 cut(s) | 484, 496 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1269 |
| BsmAI | GTCTC | 1 cut(s) | 430 |
| BsmFI | GGGAC | 2 cut(s) | 484, 496 |
| BsmI | GAATGC | 1 cut(s) | 525 |
| BsnI | GGCC | 5 cut(s) | 241, 315, 621, 1044, 1359 |
| Bsp119I | TTCGAA | 1 cut(s) | 261 |
| Bsp120I | GGGCCC | 1 cut(s) | 1357 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1197, 1361 |
| Bsp13I | TCCGGA | 1 cut(s) | 859 |
| Bsp1407I | TGTACA | 1 cut(s) | 1169 |
| Bsp143I | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| BspACI | CCGC | 4 cut(s) | 71, 801, 891, 930 |
| BspANI | GGCC | 5 cut(s) | 241, 315, 621, 1044, 1359 |
| BspCNI | CTCAG | 2 cut(s) | 152, 1195 |
| BspDI | ATCGAT | 1 cut(s) | 657 |
| BspEI | TCCGGA | 1 cut(s) | 859 |
| BspLI | GGNNCC | 3 cut(s) | 484, 1359, 1360 |
| BspPI | GGATC | 2 cut(s) | 1077, 1109 |
| BspQI | GCTCTTC | 1 cut(s) | 977 |
| BspT104I | TTCGAA | 1 cut(s) | 261 |
| BsrBI | CCGCTC | 1 cut(s) | 930 |
| BsrDI | GCAATG | 4 cut(s) | 116, 1153, 1209, 1429 |
| BsrGI | TGTACA | 1 cut(s) | 1169 |
| BsrI | ACTGG | 3 cut(s) | 384, 1222, 1445 |
| BssECI | CCNNGG | 3 cut(s) | 212, 429, 478 |
| BssMI | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| BssNAI | GTATAC | 1 cut(s) | 256 |
| BssSI | CACGAG | 1 cut(s) | 489 |
| BssT1I | CCWWGG | 3 cut(s) | 212, 429, 478 |
| Bst1107I | GTATAC | 1 cut(s) | 256 |
| Bst2BI | CACGAG | 1 cut(s) | 489 |
| Bst2UI | CCWGG | 3 cut(s) | 93, 312, 878 |
| Bst4CI | ACNGT | 6 cut(s) | 10, 176, 325, 424, 897, 1199 |
| Bst6I | CTCTTC | 4 cut(s) | 365, 705, 977, 1336 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 959 |
| BstAUI | TGTACA | 1 cut(s) | 1169 |
| BstBI | TTCGAA | 1 cut(s) | 261 |
| BstC8I | GCNNGC | 3 cut(s) | 279, 283, 832 |
| BstDEI | CTNAG | 3 cut(s) | 139, 414, 1182 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1267 |
| BstF5I | GGATG | 3 cut(s) | 211, 1317, 1441 |
| BstKTI | GATC | 8 cut(s) | 33, 192, 535, 973, 1072, 1117, 1302, 1461 |
| BstMAI | GTCTC | 1 cut(s) | 430 |
| BstMBI | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1187 |
| BstNI | CCWGG | 3 cut(s) | 93, 312, 878 |
| BstNSI | RCATGY | 2 cut(s) | 281, 743 |
| BstSCI | CCNGG | 4 cut(s) | 91, 236, 310, 876 |
| BstSFI | CTRYAG | 2 cut(s) | 705, 796 |
| BstSLI | GKGCMC | 2 cut(s) | 1197, 1361 |
| BstV1I | GCAGC | 1 cut(s) | 746 |
| BstV2I | GAAGAC | 3 cut(s) | 89, 619, 1252 |
| BstX2I | RGATCY | 2 cut(s) | 970, 1114 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 464 |
| BstYI | RGATCY | 2 cut(s) | 970, 1114 |
| BstZ17I | GTATAC | 1 cut(s) | 256 |
| Bsu15I | ATCGAT | 1 cut(s) | 657 |
| BsuI | GTATCC | 1 cut(s) | 1205 |
| BsuRI | GGCC | 5 cut(s) | 241, 315, 621, 1044, 1359 |
| BsuTUI | ATCGAT | 1 cut(s) | 657 |
| BtgZI | GCGATG | 1 cut(s) | 827 |
| BtrI | CACGTC | 2 cut(s) | 245, 617 |
| BtsCI | GGATG | 3 cut(s) | 211, 1317, 1441 |
| BtsI | GCAGTG | 2 cut(s) | 699, 1197 |
| BtsIMutI | CAGTG | 6 cut(s) | 147, 181, 699, 1197, 1215, 1234 |
| Cac8I | GCNNGC | 3 cut(s) | 279, 283, 832 |
| Cfr13I | GGNCC | 5 cut(s) | 209, 239, 483, 1357, 1358 |
| ClaI | ATCGAT | 1 cut(s) | 657 |
| CseI | GACGC | 1 cut(s) | 1140 |
| Csp6I | GTAC | 5 cut(s) | 6, 68, 904, 1108, 1170 |
| CviAII | CATG | 6 cut(s) | 278, 740, 937, 988, 1126, 1292 |
| CviQI | GTAC | 5 cut(s) | 6, 68, 904, 1108, 1170 |
| DdeI | CTNAG | 3 cut(s) | 139, 414, 1182 |
| DpnI | GATC | 8 cut(s) | 32, 191, 534, 972, 1071, 1116, 1301, 1460 |
| DpnII | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| DraIII | CACNNNGTG | 2 cut(s) | 494, 1467 |
| Eam1104I | CTCTTC | 4 cut(s) | 365, 705, 977, 1336 |
| EarI | CTCTTC | 4 cut(s) | 365, 705, 977, 1336 |
| EciI | GGCGGA | 1 cut(s) | 906 |
| Eco130I | CCWWGG | 3 cut(s) | 212, 429, 478 |
| Eco24I | GRGCYC | 1 cut(s) | 1361 |
| Eco32I | GATATC | 1 cut(s) | 454 |
| Eco47I | GGWCC | 2 cut(s) | 209, 483 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1267 |
| EcoO109I | RGGNCCY | 2 cut(s) | 483, 1358 |
| EcoRII | CCWGG | 3 cut(s) | 91, 310, 876 |
| EcoRV | GATATC | 1 cut(s) | 454 |
| EcoT14I | CCWWGG | 3 cut(s) | 212, 429, 478 |
| EcoT22I | ATGCAT | 1 cut(s) | 1015 |
| EcoT38I | GRGCYC | 1 cut(s) | 1361 |
| ErhI | CCWWGG | 3 cut(s) | 212, 429, 478 |
| FaeI | CATG | 6 cut(s) | 281, 743, 940, 991, 1129, 1295 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 140, 172, 242, 274 |
| FaqI | GGGAC | 2 cut(s) | 484, 496 |
| FatI | CATG | 6 cut(s) | 277, 739, 936, 987, 1125, 1291 |
| FauI | CCCGC | 1 cut(s) | 794 |
| FbaI | TGATCA | 1 cut(s) | 1299 |
| FblI | GTMKAC | 1 cut(s) | 255 |
| Fnu4HI | GCNGC | 1 cut(s) | 735 |
| FokI | GGATG | 3 cut(s) | 218, 1324, 1448 |
| FriOI | GRGCYC | 1 cut(s) | 1361 |
| Fsp4HI | GCNGC | 1 cut(s) | 735 |
| FspBI | CTAG | 4 cut(s) | 59, 827, 1143, 1235 |
| GluI | GCNGC | 1 cut(s) | 735 |
| GsuI | CTGGAG | 1 cut(s) | 1428 |
| HaeIII | GGCC | 5 cut(s) | 241, 315, 621, 1044, 1359 |
| HapII | CCGG | 2 cut(s) | 237, 860 |
| HgaI | GACGC | 1 cut(s) | 1140 |
| Hin1II | CATG | 6 cut(s) | 281, 743, 940, 991, 1129, 1295 |
| HincII | GTYRAC | 1 cut(s) | 520 |
| HindII | GTYRAC | 1 cut(s) | 520 |
| HindIII | AAGCTT | 1 cut(s) | 782 |
| HpaII | CCGG | 2 cut(s) | 237, 860 |
| HphI | GGTGA | 5 cut(s) | 858, 1105, 1309, 1441, 1453 |
| Hpy166II | GTNNAC | 8 cut(s) | 199, 256, 520, 553, 844, 1172, 1195, 1240 |
| Hpy188I | TCNGA | 7 cut(s) | 82, 249, 268, 773, 1083, 1156, 1185 |
| Hpy188III | TCNNGA | 6 cut(s) | 59, 489, 500, 530, 646, 860 |
| Hpy8I | GTNNAC | 8 cut(s) | 199, 256, 520, 553, 844, 1172, 1195, 1240 |
| HpyAV | CCTTC | 5 cut(s) | 98, 244, 875, 881, 950 |
| HpyCH4III | ACNGT | 6 cut(s) | 10, 176, 325, 424, 897, 1199 |
| HpyCH4IV | ACGT | 2 cut(s) | 244, 616 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1187 |
| HpyF3I | CTNAG | 3 cut(s) | 139, 414, 1182 |
| HpySE526I | ACGT | 2 cut(s) | 244, 616 |
| Hsp92II | CATG | 6 cut(s) | 281, 743, 940, 991, 1129, 1295 |
| Kpn2I | TCCGGA | 1 cut(s) | 859 |
| Ksp22I | TGATCA | 1 cut(s) | 1299 |
| Kzo9I | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| LguI | GCTCTTC | 1 cut(s) | 977 |
| LmnI | GCTCC | 1 cut(s) | 584 |
| Lsp1109I | GCAGC | 1 cut(s) | 746 |
| LweI | GCATC | 6 cut(s) | 82, 154, 596, 1000, 1022, 1071 |
| MaeI | CTAG | 4 cut(s) | 59, 827, 1143, 1235 |
| MaeII | ACGT | 2 cut(s) | 244, 616 |
| MaeIII | GTNAC | 8 cut(s) | 289, 497, 864, 932, 1019, 1053, 1415, 1447 |
| MalI | GATC | 8 cut(s) | 32, 191, 534, 972, 1071, 1116, 1301, 1460 |
| MbiI | CCGCTC | 1 cut(s) | 930 |
| MboI | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| MflI | RGATCY | 2 cut(s) | 970, 1114 |
| MhlI | GDGCHC | 2 cut(s) | 1197, 1361 |
| MluCI | AATT | 1 cut(s) | 606 |
| MlyI | GAGTC | 3 cut(s) | 428, 872, 912 |
| MmeI | TCCRAC | 1 cut(s) | 1142 |
| Mph1103I | ATGCAT | 1 cut(s) | 1015 |
| MroI | TCCGGA | 1 cut(s) | 859 |
| MseI | TTAA | 5 cut(s) | 147, 318, 729, 1032, 1077 |
| MslI | CAYNNNNRTG | 4 cut(s) | 204, 462, 671, 812 |
| MspA1I | CMGCKG | 1 cut(s) | 801 |
| MspI | CCGG | 2 cut(s) | 237, 860 |
| MspR9I | CCNGG | 4 cut(s) | 93, 238, 312, 878 |
| Mva1269I | GAATGC | 1 cut(s) | 525 |
| MvaI | CCWGG | 3 cut(s) | 93, 312, 878 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1187 |
| NciI | CCSGG | 1 cut(s) | 238 |
| NdeII | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| NlaIII | CATG | 6 cut(s) | 281, 743, 940, 991, 1129, 1295 |
| NlaIV | GGNNCC | 3 cut(s) | 484, 1359, 1360 |
| NmeAIII | GCCGAG | 2 cut(s) | 775, 1371 |
| NmuCI | GTSAC | 5 cut(s) | 497, 864, 932, 1053, 1447 |
| NsiI | ATGCAT | 1 cut(s) | 1015 |
| NspI | RCATGY | 2 cut(s) | 281, 743 |
| NspV | TTCGAA | 1 cut(s) | 261 |
| PaeI | GCATGC | 1 cut(s) | 281 |
| PciI | ACATGT | 1 cut(s) | 739 |
| PciSI | GCTCTTC | 1 cut(s) | 977 |
| PctI | GAATGC | 1 cut(s) | 525 |
| PfeI | GAWTC | 7 cut(s) | 220, 263, 540, 642, 856, 992, 1129 |
| PkrI | GCNGC | 1 cut(s) | 736 |
| PleI | GAGTC | 3 cut(s) | 428, 871, 912 |
| PpsI | GAGTC | 3 cut(s) | 428, 871, 912 |
| PpuMI | RGGWCCY | 1 cut(s) | 483 |
| PscI | ACATGT | 1 cut(s) | 739 |
| PshBI | ATTAAT | 1 cut(s) | 729 |
| Psp5II | RGGWCCY | 1 cut(s) | 483 |
| Psp6I | CCWGG | 3 cut(s) | 91, 310, 876 |
| PspGI | CCWGG | 3 cut(s) | 91, 310, 876 |
| PspN4I | GGNNCC | 3 cut(s) | 484, 1359, 1360 |
| PspOMI | GGGCCC | 1 cut(s) | 1357 |
| PspPI | GGNCC | 5 cut(s) | 209, 239, 483, 1357, 1358 |
| PspPPI | RGGWCCY | 1 cut(s) | 483 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1091, 1123 |
| PsuI | RGATCY | 2 cut(s) | 970, 1114 |
| RsaI | GTAC | 5 cut(s) | 7, 69, 905, 1109, 1171 |
| RsaNI | GTAC | 5 cut(s) | 6, 68, 904, 1108, 1170 |
| RseI | CAYNNNNRTG | 4 cut(s) | 204, 462, 671, 812 |
| SapI | GCTCTTC | 1 cut(s) | 977 |
| SaqAI | TTAA | 5 cut(s) | 147, 318, 729, 1032, 1077 |
| SatI | GCNGC | 1 cut(s) | 735 |
| Sau3AI | GATC | 8 cut(s) | 30, 189, 532, 970, 1069, 1114, 1299, 1458 |
| Sau96I | GGNCC | 5 cut(s) | 209, 239, 483, 1357, 1358 |
| ScaI | AGTACT | 1 cut(s) | 7 |
| SchI | GAGTC | 3 cut(s) | 428, 872, 912 |
| ScrFI | CCNGG | 4 cut(s) | 93, 238, 312, 878 |
| SduI | GDGCHC | 2 cut(s) | 1197, 1361 |
| SfaNI | GCATC | 6 cut(s) | 82, 154, 596, 1000, 1022, 1071 |
| SfcI | CTRYAG | 2 cut(s) | 705, 796 |
| SfuI | TTCGAA | 1 cut(s) | 261 |
| SinI | GGWCC | 2 cut(s) | 209, 483 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 204, 462, 671, 812 |
| SmlI | CTYRAG | 2 cut(s) | 920, 1103 |
| SmoI | CTYRAG | 2 cut(s) | 920, 1103 |
| SphI | GCATGC | 1 cut(s) | 281 |
| Sse9I | AATT | 1 cut(s) | 606 |
| SsiI | CCGC | 4 cut(s) | 71, 801, 891, 930 |
| SspMI | CTAG | 4 cut(s) | 59, 827, 1143, 1235 |
| StyD4I | CCNGG | 4 cut(s) | 91, 236, 310, 876 |
| StyI | CCWWGG | 3 cut(s) | 212, 429, 478 |
| TaaI | ACNGT | 6 cut(s) | 10, 176, 325, 424, 897, 1199 |
| TaiI | ACGT | 2 cut(s) | 247, 619 |
| TaqI | TCGA | 6 cut(s) | 261, 535, 657, 980, 1132, 1334 |
| TasI | AATT | 1 cut(s) | 606 |
| TatI | WGTACW | 4 cut(s) | 5, 903, 1107, 1169 |
| TfiI | GAWTC | 7 cut(s) | 220, 263, 540, 642, 856, 992, 1129 |
| Tru1I | TTAA | 5 cut(s) | 147, 318, 729, 1032, 1077 |
| Tru9I | TTAA | 5 cut(s) | 147, 318, 729, 1032, 1077 |
| TscAI | CASTG | 6 cut(s) | 147, 181, 706, 1197, 1222, 1234 |
| TseFI | GTSAC | 5 cut(s) | 497, 864, 932, 1053, 1447 |
| TseI | GCWGC | 1 cut(s) | 734 |
| Tsp45I | GTSAC | 5 cut(s) | 497, 864, 932, 1053, 1447 |
| TspDTI | ATGAA | 4 cut(s) | 212, 285, 796, 1112 |
| TspGWI | ACGGA | 2 cut(s) | 1257, 1292 |
| TspRI | CASTG | 6 cut(s) | 147, 181, 706, 1197, 1222, 1234 |
| VneI | GTGCAC | 1 cut(s) | 1193 |
| VpaK11BI | GGWCC | 2 cut(s) | 209, 483 |
| VspI | ATTAAT | 1 cut(s) | 729 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1267 |
| XbaI | TCTAGA | 1 cut(s) | 58 |
| XceI | RCATGY | 2 cut(s) | 281, 743 |
| XmiI | GTMKAC | 1 cut(s) | 255 |
| XspI | CTAG | 4 cut(s) | 59, 827, 1143, 1235 |
| ZrmI | AGTACT | 1 cut(s) | 7 |
| Zsp2I | ATGCAT | 1 cut(s) | 1015 |