Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 139 - 445 | 2.3e-62 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1013 |
| AatII | GACGTC | 1 cut(s) | 204 |
| Acc36I | ACCTGC | 1 cut(s) | 1013 |
| AccB1I | GGYRCC | 1 cut(s) | 605 |
| AccI | GTMKAC | 1 cut(s) | 1302 |
| AccII | CGCG | 1 cut(s) | 379 |
| AciI | CCGC | 6 cut(s) | 22, 70, 73, 147, 377, 528 |
| AclWI | GGATC | 4 cut(s) | 1247, 1261, 1490, 1503 |
| AcoI | YGGCCR | 3 cut(s) | 3, 631, 1066 |
| AcsI | RAATTY | 3 cut(s) | 141, 510, 777 |
| AcuI | CTGAAG | 2 cut(s) | 61, 1110 |
| AcyI | GRCGYC | 1 cut(s) | 201 |
| AdeI | CACNNNGTG | 1 cut(s) | 629 |
| AfaI | GTAC | 4 cut(s) | 107, 488, 709, 1040 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 15, 615, 976, 1567 |
| AflIII | ACRYGT | 2 cut(s) | 428, 492 |
| AgsI | TTSAA | 5 cut(s) | 96, 398, 881, 1046, 1169 |
| AjiI | CACGTC | 1 cut(s) | 368 |
| AjnI | CCWGG | 2 cut(s) | 445, 1542 |
| AluBI | AGCT | 6 cut(s) | 722, 872, 947, 1108, 1162, 1511 |
| AluI | AGCT | 6 cut(s) | 722, 872, 947, 1108, 1162, 1511 |
| Alw26I | GTCTC | 2 cut(s) | 696, 1414 |
| AlwI | GGATC | 4 cut(s) | 1247, 1261, 1490, 1503 |
| AoxI | GGCC | 4 cut(s) | 3, 631, 754, 1066 |
| ApeKI | GCWGC | 3 cut(s) | 24, 50, 869 |
| ApoI | RAATTY | 3 cut(s) | 141, 510, 777 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 806, 838 |
| AseI | ATTAAT | 2 cut(s) | 678, 864 |
| AspLEI | GCGC | 1 cut(s) | 1097 |
| AspS9I | GGNCC | 2 cut(s) | 374, 1204 |
| AsuHPI | GGTGA | 4 cut(s) | 56, 163, 1354, 1423 |
| AvaII | GGWCC | 2 cut(s) | 374, 1204 |
| BaeGI | GKGCMC | 2 cut(s) | 134, 1459 |
| BalI | TGGCCA | 2 cut(s) | 5, 633 |
| BamHI | GGATCC | 1 cut(s) | 1495 |
| BanI | GGYRCC | 1 cut(s) | 605 |
| BanII | GRGCYC | 1 cut(s) | 162 |
| BauI | CACGAG | 1 cut(s) | 624 |
| BbsI | GAAGAC | 1 cut(s) | 204 |
| BbvI | GCAGC | 3 cut(s) | 11, 37, 881 |
| BccI | CCATC | 2 cut(s) | 617, 1413 |
| BceAI | ACGGC | 2 cut(s) | 1081, 1475 |
| BciT130I | CCWGG | 2 cut(s) | 447, 1544 |
| BciVI | GTATCC | 2 cut(s) | 524, 1343 |
| BcoDI | GTCTC | 2 cut(s) | 696, 1414 |
| BfmI | CTRYAG | 1 cut(s) | 931 |
| BfuAI | ACCTGC | 1 cut(s) | 1013 |
| BfuI | GTATCC | 2 cut(s) | 524, 1343 |
| BisI | GCNGC | 4 cut(s) | 25, 51, 73, 870 |
| BlsI | GCNGC | 4 cut(s) | 26, 52, 74, 871 |
| BmcAI | AGTACT | 1 cut(s) | 107 |
| Bme1390I | CCNGG | 2 cut(s) | 447, 1544 |
| Bme18I | GGWCC | 2 cut(s) | 374, 1204 |
| BmgBI | CACGTC | 1 cut(s) | 368 |
| BmgT120I | GGNCC | 2 cut(s) | 374, 1204 |
| BmiI | GGNNCC | 3 cut(s) | 20, 607, 1497 |
| BmrFI | CCNGG | 2 cut(s) | 447, 1544 |
| BmsI | GCATC | 1 cut(s) | 1298 |
| BpiI | GAAGAC | 1 cut(s) | 204 |
| BsaHI | GRCGYC | 1 cut(s) | 201 |
| BsaJI | CCNNGG | 3 cut(s) | 14, 126, 613 |
| BsaWI | WCCGGW | 1 cut(s) | 805 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 107, 137, 711, 741 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 15, 615, 976, 1567 |
| Bse118I | RCCGGY | 1 cut(s) | 173 |
| Bse1I | ACTGG | 1 cut(s) | 1360 |
| Bse3DI | GCAATG | 2 cut(s) | 1291, 1347 |
| BseBI | CCWGG | 2 cut(s) | 447, 1544 |
| BseDI | CCNNGG | 3 cut(s) | 14, 126, 613 |
| BseGI | GGATG | 3 cut(s) | 222, 588, 609 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 15, 615, 976, 1567 |
| BseMI | GCAATG | 2 cut(s) | 1291, 1347 |
| BseNI | ACTGG | 1 cut(s) | 1360 |
| BseRI | GAGGAG | 1 cut(s) | 860 |
| BseSI | GKGCMC | 2 cut(s) | 134, 1459 |
| BseXI | GCAGC | 3 cut(s) | 11, 37, 881 |
| BsgI | GTGCAG | 1 cut(s) | 1291 |
| Bsh1236I | CGCG | 1 cut(s) | 379 |
| BshFI | GGCC | 4 cut(s) | 5, 633, 756, 1068 |
| BshNI | GGYRCC | 1 cut(s) | 605 |
| BsiSI | CCGG | 2 cut(s) | 174, 806 |
| BslFI | GGGAC | 2 cut(s) | 188, 631 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 15, 615, 976, 1567 |
| BsmAI | GTCTC | 2 cut(s) | 696, 1414 |
| BsmBI | CGTCTC | 1 cut(s) | 696 |
| BsmFI | GGGAC | 2 cut(s) | 188, 631 |
| BsmI | GAATGC | 2 cut(s) | 660, 746 |
| BsnI | GGCC | 4 cut(s) | 5, 633, 756, 1068 |
| Bsp1286I | GDGCHC | 3 cut(s) | 134, 162, 1459 |
| Bsp143I | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| BspACI | CCGC | 6 cut(s) | 22, 70, 73, 147, 377, 528 |
| BspANI | GGCC | 4 cut(s) | 5, 633, 756, 1068 |
| BspFNI | CGCG | 1 cut(s) | 379 |
| BspLI | GGNNCC | 3 cut(s) | 20, 607, 1497 |
| BspMI | ACCTGC | 1 cut(s) | 1013 |
| BspPI | GGATC | 4 cut(s) | 1247, 1261, 1490, 1503 |
| BspT107I | GGYRCC | 1 cut(s) | 605 |
| BsrDI | GCAATG | 2 cut(s) | 1291, 1347 |
| BsrFI | RCCGGY | 1 cut(s) | 173 |
| BsrI | ACTGG | 1 cut(s) | 1360 |
| BssAI | RCCGGY | 1 cut(s) | 173 |
| BssECI | CCNNGG | 3 cut(s) | 14, 126, 613 |
| BssMI | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| BssNAI | GTATAC | 1 cut(s) | 1303 |
| BssNI | GRCGYC | 1 cut(s) | 201 |
| BssSI | CACGAG | 1 cut(s) | 624 |
| BssT1I | CCWWGG | 2 cut(s) | 126, 613 |
| Bst1107I | GTATAC | 1 cut(s) | 1303 |
| Bst2BI | CACGAG | 1 cut(s) | 624 |
| Bst2UI | CCWGG | 2 cut(s) | 447, 1544 |
| Bst4CI | ACNGT | 5 cut(s) | 18, 460, 707, 935, 1366 |
| BstACI | GRCGYC | 1 cut(s) | 201 |
| BstC8I | GCNNGC | 5 cut(s) | 184, 967, 1124, 1128, 1389 |
| BstDSI | CCRYGG | 1 cut(s) | 14 |
| BstF5I | GGATG | 3 cut(s) | 222, 588, 609 |
| BstFNI | CGCG | 1 cut(s) | 379 |
| BstHHI | GCGC | 1 cut(s) | 1097 |
| BstKTI | GATC | 6 cut(s) | 64, 168, 232, 1255, 1269, 1498 |
| BstMAI | GTCTC | 2 cut(s) | 696, 1414 |
| BstMBI | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 78, 183, 857, 866, 953, 962, 1094 |
| BstNI | CCWGG | 2 cut(s) | 447, 1544 |
| BstNSI | RCATGY | 4 cut(s) | 420, 432, 496, 1391 |
| BstSCI | CCNGG | 2 cut(s) | 445, 1542 |
| BstSFI | CTRYAG | 1 cut(s) | 931 |
| BstSLI | GKGCMC | 2 cut(s) | 134, 1459 |
| BstUI | CGCG | 1 cut(s) | 379 |
| BstV1I | GCAGC | 3 cut(s) | 11, 37, 881 |
| BstV2I | GAAGAC | 1 cut(s) | 204 |
| BstX2I | RGATCY | 2 cut(s) | 1252, 1495 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 599 |
| BstYI | RGATCY | 2 cut(s) | 1252, 1495 |
| BstZ17I | GTATAC | 1 cut(s) | 1303 |
| BsuI | GTATCC | 2 cut(s) | 524, 1343 |
| BsuRI | GGCC | 4 cut(s) | 5, 633, 756, 1068 |
| BtgI | CCRYGG | 1 cut(s) | 14 |
| BtrI | CACGTC | 1 cut(s) | 368 |
| BtsCI | GGATG | 3 cut(s) | 222, 588, 609 |
| BtsI | GCAGTG | 2 cut(s) | 774, 834 |
| BtsIMutI | CAGTG | 4 cut(s) | 774, 834, 940, 1353 |
| BveI | ACCTGC | 1 cut(s) | 1013 |
| Cac8I | GCNNGC | 5 cut(s) | 184, 967, 1124, 1128, 1389 |
| CfoI | GCGC | 1 cut(s) | 1097 |
| Cfr10I | RCCGGY | 1 cut(s) | 173 |
| Cfr13I | GGNCC | 2 cut(s) | 374, 1204 |
| CseI | GACGC | 3 cut(s) | 188, 368, 1154 |
| CsiI | ACCWGGT | 1 cut(s) | 1542 |
| Csp6I | GTAC | 4 cut(s) | 106, 487, 708, 1039 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 633, 668 |
| CviAII | CATG | 9 cut(s) | 417, 429, 493, 635, 875, 1072, 1264, 1334, 1388 |
| CviQI | GTAC | 4 cut(s) | 106, 487, 708, 1039 |
| DpnI | GATC | 6 cut(s) | 63, 167, 231, 1254, 1268, 1497 |
| DpnII | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| DraIII | CACNNNGTG | 1 cut(s) | 629 |
| EaeI | YGGCCR | 3 cut(s) | 3, 631, 1066 |
| EciI | GGCGGA | 2 cut(s) | 136, 543 |
| Eco130I | CCWWGG | 2 cut(s) | 126, 613 |
| Eco24I | GRGCYC | 1 cut(s) | 162 |
| Eco47I | GGWCC | 2 cut(s) | 374, 1204 |
| Eco57I | CTGAAG | 2 cut(s) | 61, 1110 |
| EcoRI | GAATTC | 2 cut(s) | 510, 777 |
| EcoRII | CCWGG | 2 cut(s) | 445, 1542 |
| EcoT14I | CCWWGG | 2 cut(s) | 126, 613 |
| EcoT22I | ATGCAT | 1 cut(s) | 1291 |
| EcoT38I | GRGCYC | 1 cut(s) | 162 |
| ErhI | CCWWGG | 2 cut(s) | 126, 613 |
| Esp3I | CGTCTC | 1 cut(s) | 696 |
| FaeI | CATG | 9 cut(s) | 420, 432, 496, 638, 878, 1075, 1267, 1337, 1391 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1095, 1127, 1193, 1225, 1375, 1407 |
| FaqI | GGGAC | 2 cut(s) | 188, 631 |
| FatI | CATG | 9 cut(s) | 416, 428, 492, 634, 874, 1071, 1263, 1333, 1387 |
| FauI | CCCGC | 1 cut(s) | 77 |
| FblI | GTMKAC | 1 cut(s) | 1302 |
| Fnu4HI | GCNGC | 4 cut(s) | 25, 51, 73, 870 |
| FokI | GGATG | 3 cut(s) | 209, 575, 596 |
| FriOI | GRGCYC | 1 cut(s) | 162 |
| Fsp4HI | GCNGC | 4 cut(s) | 25, 51, 73, 870 |
| GlaI | GCGC | 1 cut(s) | 1096 |
| GluI | GCNGC | 4 cut(s) | 25, 51, 73, 870 |
| HaeIII | GGCC | 4 cut(s) | 5, 633, 756, 1068 |
| HapII | CCGG | 2 cut(s) | 174, 806 |
| HgaI | GACGC | 3 cut(s) | 188, 368, 1154 |
| HhaI | GCGC | 1 cut(s) | 1097 |
| Hin1I | GRCGYC | 1 cut(s) | 201 |
| Hin1II | CATG | 9 cut(s) | 420, 432, 496, 638, 878, 1075, 1267, 1337, 1391 |
| Hin6I | GCGC | 1 cut(s) | 1095 |
| HinP1I | GCGC | 1 cut(s) | 1095 |
| HindIII | AAGCTT | 1 cut(s) | 1160 |
| HinfI | GANTC | 4 cut(s) | 991, 998, 1135, 1172 |
| HpaII | CCGG | 2 cut(s) | 174, 806 |
| HphI | GGTGA | 4 cut(s) | 56, 163, 1354, 1423 |
| Hpy166II | GTNNAC | 5 cut(s) | 505, 655, 688, 710, 1303 |
| Hpy188I | TCNGA | 5 cut(s) | 157, 217, 384, 1294, 1500 |
| Hpy8I | GTNNAC | 5 cut(s) | 505, 655, 688, 710, 1303 |
| Hpy99I | CGWCG | 3 cut(s) | 182, 369, 1370 |
| HpyAV | CCTTC | 6 cut(s) | 276, 379, 1010, 1085, 1377, 1495 |
| HpyCH4III | ACNGT | 5 cut(s) | 18, 460, 707, 935, 1366 |
| HpyCH4IV | ACGT | 4 cut(s) | 189, 201, 367, 598 |
| HpyCH4V | TGCA | 7 cut(s) | 416, 744, 767, 869, 1289, 1308, 1340 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 78, 183, 857, 866, 953, 962, 1094 |
| HpySE526I | ACGT | 4 cut(s) | 189, 201, 367, 598 |
| Hsp92I | GRCGYC | 1 cut(s) | 201 |
| Hsp92II | CATG | 9 cut(s) | 420, 432, 496, 638, 878, 1075, 1267, 1337, 1391 |
| HspAI | GCGC | 1 cut(s) | 1095 |
| Kzo9I | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| LmnI | GCTCC | 2 cut(s) | 719, 1019 |
| Lsp1109I | GCAGC | 3 cut(s) | 11, 37, 881 |
| LweI | GCATC | 1 cut(s) | 1298 |
| MabI | ACCWGGT | 1 cut(s) | 1542 |
| MaeII | ACGT | 4 cut(s) | 189, 201, 367, 598 |
| MaeIII | GTNAC | 2 cut(s) | 424, 1411 |
| MalI | GATC | 6 cut(s) | 63, 167, 231, 1254, 1268, 1497 |
| MboI | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| MboII | GAAGA | 4 cut(s) | 209, 1157, 1207, 1538 |
| MflI | RGATCY | 2 cut(s) | 1252, 1495 |
| MhlI | GDGCHC | 3 cut(s) | 134, 162, 1459 |
| MlsI | TGGCCA | 2 cut(s) | 5, 633 |
| MluCI | AATT | 8 cut(s) | 91, 141, 462, 510, 558, 675, 777, 1627 |
| MluNI | TGGCCA | 2 cut(s) | 5, 633 |
| MlyI | GAGTC | 1 cut(s) | 1007 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 633 |
| Mph1103I | ATGCAT | 1 cut(s) | 1291 |
| MscI | TGGCCA | 2 cut(s) | 5, 633 |
| MslI | CAYNNNNRTG | 1 cut(s) | 597 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 633 |
| MspA1I | CMGCKG | 1 cut(s) | 24 |
| MspI | CCGG | 2 cut(s) | 174, 806 |
| MspR9I | CCNGG | 2 cut(s) | 447, 1544 |
| Mva1269I | GAATGC | 2 cut(s) | 660, 746 |
| MvaI | CCWGG | 2 cut(s) | 447, 1544 |
| MvnI | CGCG | 1 cut(s) | 379 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 78, 183, 857, 866, 953, 962, 1094 |
| NdeII | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| NlaIII | CATG | 9 cut(s) | 420, 432, 496, 638, 878, 1075, 1267, 1337, 1391 |
| NlaIV | GGNNCC | 3 cut(s) | 20, 607, 1497 |
| NmeAIII | GCCGAG | 1 cut(s) | 995 |
| NmuCI | GTSAC | 2 cut(s) | 424, 1411 |
| NsiI | ATGCAT | 1 cut(s) | 1291 |
| NspI | RCATGY | 4 cut(s) | 420, 432, 496, 1391 |
| PaeI | GCATGC | 1 cut(s) | 1391 |
| PaqCI | CACCTGC | 1 cut(s) | 1013 |
| PciI | ACATGT | 2 cut(s) | 428, 492 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 186, 300 |
| PctI | GAATGC | 2 cut(s) | 660, 746 |
| PfeI | GAWTC | 3 cut(s) | 991, 1135, 1172 |
| PflFI | GACNNNGTC | 1 cut(s) | 378 |
| PkrI | GCNGC | 4 cut(s) | 26, 52, 74, 871 |
| PleI | GAGTC | 1 cut(s) | 1006 |
| PpsI | GAGTC | 1 cut(s) | 1006 |
| PscI | ACATGT | 2 cut(s) | 428, 492 |
| PshBI | ATTAAT | 2 cut(s) | 678, 864 |
| Psp6I | CCWGG | 2 cut(s) | 445, 1542 |
| PspGI | CCWGG | 2 cut(s) | 445, 1542 |
| PspN4I | GGNNCC | 3 cut(s) | 20, 607, 1497 |
| PspPI | GGNCC | 2 cut(s) | 374, 1204 |
| PsuI | RGATCY | 2 cut(s) | 1252, 1495 |
| PsyI | GACNNNGTC | 1 cut(s) | 378 |
| RsaI | GTAC | 4 cut(s) | 107, 488, 709, 1040 |
| RsaNI | GTAC | 4 cut(s) | 106, 487, 708, 1039 |
| RseI | CAYNNNNRTG | 1 cut(s) | 597 |
| SatI | GCNGC | 4 cut(s) | 25, 51, 73, 870 |
| Sau3AI | GATC | 6 cut(s) | 61, 165, 229, 1252, 1266, 1495 |
| Sau96I | GGNCC | 2 cut(s) | 374, 1204 |
| ScaI | AGTACT | 1 cut(s) | 107 |
| SchI | GAGTC | 1 cut(s) | 1007 |
| ScrFI | CCNGG | 2 cut(s) | 447, 1544 |
| SduI | GDGCHC | 3 cut(s) | 134, 162, 1459 |
| SexAI | ACCWGGT | 1 cut(s) | 1542 |
| SfaNI | GCATC | 1 cut(s) | 1298 |
| SfcI | CTRYAG | 1 cut(s) | 931 |
| SgrAI | CRCCGGYG | 1 cut(s) | 173 |
| SinI | GGWCC | 2 cut(s) | 374, 1204 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 597 |
| SphI | GCATGC | 1 cut(s) | 1391 |
| Sse9I | AATT | 8 cut(s) | 91, 141, 462, 510, 558, 675, 777, 1627 |
| SsiI | CCGC | 6 cut(s) | 22, 70, 73, 147, 377, 528 |
| SspI | AATATT | 1 cut(s) | 732 |
| StyD4I | CCNGG | 2 cut(s) | 445, 1542 |
| StyI | CCWWGG | 2 cut(s) | 126, 613 |
| TaaI | ACNGT | 5 cut(s) | 18, 460, 707, 935, 1366 |
| TaiI | ACGT | 4 cut(s) | 192, 204, 370, 601 |
| TaqI | TCGA | 4 cut(s) | 30, 195, 303, 364 |
| TasI | AATT | 8 cut(s) | 91, 141, 462, 510, 558, 675, 777, 1627 |
| TatI | WGTACW | 3 cut(s) | 105, 486, 1038 |
| TauI | GCSGC | 1 cut(s) | 75 |
| TfiI | GAWTC | 3 cut(s) | 991, 1135, 1172 |
| TscAI | CASTG | 4 cut(s) | 774, 841, 940, 1360 |
| TseFI | GTSAC | 2 cut(s) | 424, 1411 |
| TseI | GCWGC | 3 cut(s) | 24, 50, 869 |
| Tsp45I | GTSAC | 2 cut(s) | 424, 1411 |
| TspDTI | ATGAA | 2 cut(s) | 1148, 1322 |
| TspGWI | ACGGA | 2 cut(s) | 1259, 1430 |
| TspRI | CASTG | 4 cut(s) | 774, 841, 940, 1360 |
| Tth111I | GACNNNGTC | 1 cut(s) | 378 |
| VpaK11BI | GGWCC | 2 cut(s) | 374, 1204 |
| VspI | ATTAAT | 2 cut(s) | 678, 864 |
| XapI | RAATTY | 3 cut(s) | 141, 510, 777 |
| XceI | RCATGY | 4 cut(s) | 420, 432, 496, 1391 |
| XcmI | CCANNNNNNNNNTGG | 5 cut(s) | 133, 512, 599, 973, 1547 |
| XmiI | GTMKAC | 1 cut(s) | 1302 |
| ZraI | GACGTC | 1 cut(s) | 202 |
| ZrmI | AGTACT | 1 cut(s) | 107 |
| Zsp2I | ATGCAT | 1 cut(s) | 1291 |