Rroxscaffold_2G00104240

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000762
Physical Location & Seq
Reverse (-)
27319599 .. 27325537
5939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104240.1

Sequence Viewer

Length: 1671 bp
ATGAGAATGGCCAACCCTGCTTCTTCCATTTCTTCCACCACTCCTCGGTTCCGCTGCTTCGACCCCACCAACGCCTACGGTGCGACGCCCCATCACCCCCGCCGCTTCAGCACCTCCTCCAATTCAACTCCCAGGAACTACAACAACAACAACGACAACAACAACTCCAAGGGCACTATGGGAATTTCCGCCAACTCCCAGCCCAAGATCGTCCCCGGCGACGCTGGCTACGTTCTCGAAGACGTCCCTCACTTGTCCGATTACATCCCCGATCTCCCTGCTGCTCGATCTGCTCGCTCACAATTACTTACATATCCCAATCCATTACAGGACAATCCTTCCTATTCAGTTGTTAAGCAGTATTTCGTTAATGTCGATGATAGTGTTGCCCAGACGATAGTAGTCCAGAAAGATAGTCCAAGAGGCACACATTTTCGTCGTGCTGGACCGCGTCAGAAGGTCTATTTTGAACCAGATGAGGTTCATGCGTGTATTGTGACATGTGGTGGTTTATGTCCAGGCTTAAATACAGTAATTAGAGAAATAGTATGTGGGTTGTACCACATGTATGGTGTCCACCGAATTCTTGGAATAGAGGGAGGATACAGAGGATTTTATGCCAGAAATACAATTCCCTTAACGCCAAAGGTTGTGAATGACATCCATAAACGTGGTGGCACCATCCTTGGGACATCACGAGGTGGCCATGATACCTCAAAGATTGTGGACAGCATTCAGGACAGAGGAATTAATCAGGTTTACATACTCGGGGGAGATGGTACACAGAAAGGAGCTTCCGTAATATTTGAGGAATGCAGGAGGCGTGGCCTGAAAGTTGCAGTGGCAGGAATTCCAAAAACCATTGATAATGACATTCCGGTTATAGACAAGTCCTTTGGTTTTGACACTGCTGTTGAGGAGGCTCAACGAGCCATTAATGCAGCTCATGTTGAAGCAGAAAGCATTGAGAATGGTATTGGCGTTGTCAAGTTAATGGGTCGCTACAGTGGATTTATAGCTATGTATGCTACTCTTGCCAGCCGAGATGTGGATTGTTGCCTGATTCCTGAGTCGCCTTTCTATCTGGAAGGAGCAGGTGGACTGTTTGAATACATTGAAAAAAGACTTAAAGAGAATGGCCACATGGTTATAGTAATAGCTGAAGGCACAGGACAAGAGCTTCTAGCTGAAAGCATGCTTGCCATGAATCATCAGGACGCTTCTGGAAATAAGCTTCTTCAAGATTCTGGTCTGTGGTTATCACAGAAGATTAAGGACCACTTCTCAAAACTGAACAAGATGTCTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCTATTCCAAGCAATGCATCTGATAATGTGTACTGCACCCTCCTTGCTCATAGTGTGGTTCATGGTGCAATGGCAGGATACACCGGCTTCACTGTCGGTTCTGTTAATGGAAGGCATGCTTATATTCCTTTCCATCGGGTGACGGAGAGACAGAACAAGGTTGTGATTACTGACAGAATGTGGGCACGGCTATTATCATCAACTAATCAGCCAAGTTTCTTGGATTTGAAGGAAGTAGCTAAAAAGAAAGGGGAAGAAACAGAAACCCACCTGGTCAATGGGGAAAACCATTTGGAGAAAACTGCTTTGGCTACGCCATTGGAGTAG

Protein Analysis

556

Amino Acids

61.07

Weight (kDa)

7.99

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 164 - 469 1e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1085
AatII GACGTC 1 cut(s) 246
Acc36I ACCTGC 1 cut(s) 1085
AccB1I GGYRCC 1 cut(s) 677
AccII CGCG 1 cut(s) 451
AciI CCGC 5 cut(s) 52, 100, 103, 189, 449
AclWI GGATC 2 cut(s) 1319, 1333
AcoI YGGCCR 3 cut(s) 9, 703, 1138
AcsI RAATTY 3 cut(s) 183, 582, 849
AcuI CTGAAG 2 cut(s) 91, 1182
AcyI GRCGYC 2 cut(s) 86, 243
AdeI CACNNNGTG 1 cut(s) 701
AfaI GTAC 3 cut(s) 560, 781, 1376
AfiI CCNNNNNNNGG 3 cut(s) 45, 687, 1048
AflIII ACRYGT 2 cut(s) 500, 564
AgsI TTSAA 7 cut(s) 126, 470, 953, 1109, 1118, 1241, 1573
AjnI CCWGG 3 cut(s) 131, 517, 1614
AjuI GAANNNNNNNTTGG 2 cut(s) 1634, 1666
AluBI AGCT 8 cut(s) 794, 944, 1019, 1160, 1180, 1187, 1234, 1583
AluI AGCT 8 cut(s) 794, 944, 1019, 1160, 1180, 1187, 1234, 1583
Alw26I GTCTC 1 cut(s) 1486
AlwI GGATC 2 cut(s) 1319, 1333
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 4 cut(s) 9, 703, 826, 1138
ApeKI GCWGC 3 cut(s) 54, 281, 941
ApoI RAATTY 3 cut(s) 183, 582, 849
ArsI GACNNNNNNTTYG 2 cut(s) 878, 910
AseI ATTAAT 2 cut(s) 750, 936
AspS9I GGNCC 2 cut(s) 446, 1276
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 2 cut(s) 86, 1495
AvaI CYCGRG 1 cut(s) 767
AvaII GGWCC 2 cut(s) 446, 1276
BaeGI GKGCMC 2 cut(s) 176, 1531
BalI TGGCCA 3 cut(s) 11, 705, 1140
BanI GGYRCC 1 cut(s) 677
BauI CACGAG 1 cut(s) 696
BbsI GAAGAC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 41, 268, 953
BccI CCATC 4 cut(s) 99, 689, 770, 1485
BceAI ACGGC 1 cut(s) 1547
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 3 cut(s) 133, 519, 1616
BciVI GTATCC 2 cut(s) 596, 1415
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 1486
BfaI CTAG 1 cut(s) 1184
BfmI CTRYAG 1 cut(s) 1003
BfuAI ACCTGC 1 cut(s) 1085
BfuI GTATCC 2 cut(s) 596, 1415
BisI GCNGC 4 cut(s) 55, 103, 282, 942
BlsI GCNGC 4 cut(s) 56, 104, 283, 943
Bme1390I CCNGG 4 cut(s) 133, 216, 519, 1616
Bme18I GGWCC 2 cut(s) 446, 1276
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 2 cut(s) 446, 1276
BmiI GGNNCC 2 cut(s) 50, 679
BmrFI CCNGG 4 cut(s) 133, 216, 519, 1616
BmsI GCATC 1 cut(s) 1370
BpiI GAAGAC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 216
BsaHI GRCGYC 2 cut(s) 86, 243
BsaJI CCNNGG 5 cut(s) 44, 131, 168, 214, 685
BsaWI WCCGGW 1 cut(s) 877
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 783, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 687, 1048
Bse118I RCCGGY 1 cut(s) 1427
Bse3DI GCAATG 2 cut(s) 1363, 1419
BseBI CCWGG 3 cut(s) 133, 519, 1616
BseDI CCNNGG 5 cut(s) 44, 131, 168, 214, 685
BseGI GGATG 3 cut(s) 264, 660, 681
BseLI CCNNNNNNNGG 3 cut(s) 45, 687, 1048
BseMI GCAATG 2 cut(s) 1363, 1419
BseMII CTCAG 1 cut(s) 1059
BseRI GAGGAG 3 cut(s) 33, 106, 932
BseSI GKGCMC 2 cut(s) 176, 1531
BseXI GCAGC 3 cut(s) 41, 268, 953
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 1363
Bsh1236I CGCG 1 cut(s) 451
BshFI GGCC 4 cut(s) 11, 705, 828, 1140
BshNI GGYRCC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 3 cut(s) 216, 878, 1428
BslFI GGGAC 3 cut(s) 197, 230, 703
BslI CCNNNNNNNGG 3 cut(s) 45, 687, 1048
BsmAI GTCTC 1 cut(s) 1486
BsmFI GGGAC 3 cut(s) 197, 230, 703
BsmI GAATGC 2 cut(s) 732, 818
BsnI GGCC 4 cut(s) 11, 705, 828, 1140
BsoBI CYCGRG 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 176, 1531
Bsp143I GATC 5 cut(s) 207, 271, 287, 1324, 1338
BspACI CCGC 5 cut(s) 52, 100, 103, 189, 449
BspANI GGCC 4 cut(s) 11, 705, 828, 1140
BspCNI CTCAG 1 cut(s) 1060
BspFNI CGCG 1 cut(s) 451
BspLI GGNNCC 2 cut(s) 50, 679
BspMI ACCTGC 1 cut(s) 1085
BspPI GGATC 2 cut(s) 1319, 1333
BspT107I GGYRCC 1 cut(s) 677
BsrDI GCAATG 2 cut(s) 1363, 1419
BsrFI RCCGGY 1 cut(s) 1427
BssAI RCCGGY 1 cut(s) 1427
BssECI CCNNGG 5 cut(s) 44, 131, 168, 214, 685
BssMI GATC 5 cut(s) 207, 271, 287, 1324, 1338
BssNI GRCGYC 2 cut(s) 86, 243
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 2 cut(s) 168, 685
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 3 cut(s) 133, 519, 1616
Bst4CI ACNGT 5 cut(s) 80, 532, 1007, 1104, 1438
BstACI GRCGYC 2 cut(s) 86, 243
BstC8I GCNNGC 6 cut(s) 226, 295, 1039, 1196, 1200, 1461
BstDEI CTNAG 1 cut(s) 1068
BstF5I GGATG 3 cut(s) 264, 660, 681
BstFNI CGCG 1 cut(s) 451
BstKTI GATC 5 cut(s) 210, 274, 290, 1327, 1341
BstMAI GTCTC 1 cut(s) 1486
BstMBI GATC 5 cut(s) 207, 271, 287, 1324, 1338
BstMWI GCNNNNNNNGC 9 cut(s) 17, 80, 108, 225, 290, 929, 938, 1025, 1034
BstNI CCWGG 3 cut(s) 133, 519, 1616
BstNSI RCATGY 4 cut(s) 504, 568, 1198, 1463
BstSCI CCNGG 4 cut(s) 131, 214, 517, 1614
BstSFI CTRYAG 1 cut(s) 1003
BstSLI GKGCMC 2 cut(s) 176, 1531
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 3 cut(s) 41, 268, 953
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 1324
BstXI CCANNNNNNTGG 2 cut(s) 569, 671
BstYI RGATCY 1 cut(s) 1324
BsuI GTATCC 2 cut(s) 596, 1415
BsuRI GGCC 4 cut(s) 11, 705, 828, 1140
BtsCI GGATG 3 cut(s) 264, 660, 681
BtsI GCAGTG 2 cut(s) 846, 906
BtsIMutI CAGTG 4 cut(s) 846, 906, 1012, 1434
BveI ACCTGC 1 cut(s) 1085
Cac8I GCNNGC 6 cut(s) 226, 295, 1039, 1196, 1200, 1461
Cfr10I RCCGGY 1 cut(s) 1427
Cfr13I GGNCC 2 cut(s) 446, 1276
CseI GACGC 4 cut(s) 94, 230, 440, 1226
CsiI ACCWGGT 1 cut(s) 1614
Csp6I GTAC 3 cut(s) 559, 780, 1375
CspCI CAANNNNNGTGG 2 cut(s) 705, 740
CviQI GTAC 3 cut(s) 559, 780, 1375
DdeI CTNAG 1 cut(s) 1068
DpnI GATC 5 cut(s) 209, 273, 289, 1326, 1340
DpnII GATC 5 cut(s) 207, 271, 287, 1324, 1338
DraIII CACNNNGTG 1 cut(s) 701
EaeI YGGCCR 3 cut(s) 9, 703, 1138
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 168, 685
Eco47I GGWCC 2 cut(s) 446, 1276
Eco57I CTGAAG 2 cut(s) 91, 1182
Eco88I CYCGRG 1 cut(s) 767
EcoRI GAATTC 2 cut(s) 582, 849
EcoRII CCWGG 3 cut(s) 131, 517, 1614
EcoT14I CCWWGG 2 cut(s) 168, 685
EcoT22I ATGCAT 1 cut(s) 1363
ErhI CCWWGG 2 cut(s) 168, 685
FalI AAGNNNNNCTT 4 cut(s) 1265, 1297, 1447, 1479
FaqI GGGAC 3 cut(s) 197, 230, 703
FauI CCCGC 1 cut(s) 107
Fnu4HI GCNGC 4 cut(s) 55, 103, 282, 942
FokI GGATG 3 cut(s) 251, 647, 668
Fsp4HI GCNGC 4 cut(s) 55, 103, 282, 942
FspBI CTAG 1 cut(s) 1184
GluI GCNGC 4 cut(s) 55, 103, 282, 942
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 4 cut(s) 11, 705, 828, 1140
HapII CCGG 3 cut(s) 216, 878, 1428
HgaI GACGC 4 cut(s) 94, 230, 440, 1226
Hin1I GRCGYC 2 cut(s) 86, 243
HindIII AAGCTT 1 cut(s) 1232
HinfI GANTC 4 cut(s) 1063, 1070, 1207, 1244
HpaII CCGG 3 cut(s) 216, 878, 1428
HphI GGTGA 2 cut(s) 86, 1495
Hpy166II GTNNAC 6 cut(s) 577, 727, 760, 782, 1100, 1375
Hpy188I TCNGA 3 cut(s) 259, 456, 1366
Hpy188III TCNNGA 9 cut(s) 236, 406, 696, 737, 1067, 1085, 1214, 1224, 1241
Hpy8I GTNNAC 6 cut(s) 577, 727, 760, 782, 1100, 1375
Hpy99I CGWCG 3 cut(s) 88, 224, 441
HpyAV CCTTC 6 cut(s) 348, 451, 1082, 1157, 1449, 1567
HpyCH4III ACNGT 5 cut(s) 80, 532, 1007, 1104, 1438
HpyCH4IV ACGT 3 cut(s) 231, 243, 670
HpyCH4V TGCA 6 cut(s) 816, 839, 941, 1361, 1380, 1412
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 80, 108, 225, 290, 929, 938, 1025, 1034
HpyF3I CTNAG 1 cut(s) 1068
HpySE526I ACGT 3 cut(s) 231, 243, 670
Hsp92I GRCGYC 2 cut(s) 86, 243
Kzo9I GATC 5 cut(s) 207, 271, 287, 1324, 1338
LmnI GCTCC 2 cut(s) 791, 1091
Lsp1109I GCAGC 3 cut(s) 41, 268, 953
LweI GCATC 1 cut(s) 1370
MabI ACCWGGT 1 cut(s) 1614
MaeI CTAG 1 cut(s) 1184
MaeII ACGT 3 cut(s) 231, 243, 670
MaeIII GTNAC 2 cut(s) 496, 1483
MalI GATC 5 cut(s) 209, 273, 289, 1326, 1340
MboI GATC 5 cut(s) 207, 271, 287, 1324, 1338
MboII GAAGA 6 cut(s) 15, 24, 251, 1229, 1279, 1610
MflI RGATCY 1 cut(s) 1324
MhlI GDGCHC 2 cut(s) 176, 1531
MlsI TGGCCA 3 cut(s) 11, 705, 1140
MluCI AATT 8 cut(s) 121, 183, 302, 534, 582, 630, 747, 849
MluNI TGGCCA 3 cut(s) 11, 705, 1140
MlyI GAGTC 1 cut(s) 1079
Mox20I TGGCCA 3 cut(s) 11, 705, 1140
Mph1103I ATGCAT 1 cut(s) 1363
MscI TGGCCA 3 cut(s) 11, 705, 1140
MslI CAYNNNNRTG 2 cut(s) 567, 669
Msp20I TGGCCA 3 cut(s) 11, 705, 1140
MspA1I CMGCKG 1 cut(s) 54
MspI CCGG 3 cut(s) 216, 878, 1428
MspR9I CCNGG 4 cut(s) 133, 216, 519, 1616
Mva1269I GAATGC 2 cut(s) 732, 818
MvaI CCWGG 3 cut(s) 133, 519, 1616
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 9 cut(s) 17, 80, 108, 225, 290, 929, 938, 1025, 1034
NciI CCSGG 1 cut(s) 216
NdeII GATC 5 cut(s) 207, 271, 287, 1324, 1338
NlaIV GGNNCC 2 cut(s) 50, 679
NmeAIII GCCGAG 1 cut(s) 1067
NmuCI GTSAC 2 cut(s) 496, 1483
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 4 cut(s) 504, 568, 1198, 1463
PaeI GCATGC 2 cut(s) 1198, 1463
PaqCI CACCTGC 1 cut(s) 1085
PciI ACATGT 2 cut(s) 500, 564
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 228, 372
PctI GAATGC 2 cut(s) 732, 818
PfeI GAWTC 3 cut(s) 1063, 1207, 1244
PflFI GACNNNGTC 1 cut(s) 450
PkrI GCNGC 4 cut(s) 56, 104, 283, 943
PleI GAGTC 1 cut(s) 1078
PpsI GAGTC 1 cut(s) 1078
PscI ACATGT 2 cut(s) 500, 564
PshBI ATTAAT 2 cut(s) 750, 936
Psp6I CCWGG 3 cut(s) 131, 517, 1614
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 3 cut(s) 131, 517, 1614
PspN4I GGNNCC 2 cut(s) 50, 679
PspPI GGNCC 2 cut(s) 446, 1276
PsuI RGATCY 1 cut(s) 1324
PsyI GACNNNGTC 1 cut(s) 450
RsaI GTAC 3 cut(s) 560, 781, 1376
RsaNI GTAC 3 cut(s) 559, 780, 1375
RseI CAYNNNNRTG 2 cut(s) 567, 669
SatI GCNGC 4 cut(s) 55, 103, 282, 942
Sau3AI GATC 5 cut(s) 207, 271, 287, 1324, 1338
Sau96I GGNCC 2 cut(s) 446, 1276
SchI GAGTC 1 cut(s) 1079
ScrFI CCNGG 4 cut(s) 133, 216, 519, 1616
SduI GDGCHC 2 cut(s) 176, 1531
SexAI ACCWGGT 1 cut(s) 1614
SfaNI GCATC 1 cut(s) 1370
SfcI CTRYAG 1 cut(s) 1003
SinI GGWCC 2 cut(s) 446, 1276
SmiMI CAYNNNNRTG 2 cut(s) 567, 669
SphI GCATGC 2 cut(s) 1198, 1463
Sse9I AATT 8 cut(s) 121, 183, 302, 534, 582, 630, 747, 849
SsiI CCGC 5 cut(s) 52, 100, 103, 189, 449
SspI AATATT 1 cut(s) 804
SspMI CTAG 1 cut(s) 1184
StyD4I CCNGG 4 cut(s) 131, 214, 517, 1614
StyI CCWWGG 2 cut(s) 168, 685
TaaI ACNGT 5 cut(s) 80, 532, 1007, 1104, 1438
TaiI ACGT 3 cut(s) 234, 246, 673
TaqI TCGA 4 cut(s) 60, 237, 286, 375
TasI AATT 8 cut(s) 121, 183, 302, 534, 582, 630, 747, 849
TatI WGTACW 1 cut(s) 1374
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 3 cut(s) 1063, 1207, 1244
TscAI CASTG 4 cut(s) 846, 913, 1012, 1441
TseFI GTSAC 2 cut(s) 496, 1483
TseI GCWGC 3 cut(s) 54, 281, 941
Tsp45I GTSAC 2 cut(s) 496, 1483
TspDTI ATGAA 3 cut(s) 473, 1220, 1394
TspGWI ACGGA 3 cut(s) 787, 1331, 1502
TspRI CASTG 4 cut(s) 846, 913, 1012, 1441
Tth111I GACNNNGTC 1 cut(s) 450
VpaK11BI GGWCC 2 cut(s) 446, 1276
VspI ATTAAT 2 cut(s) 750, 936
XapI RAATTY 3 cut(s) 183, 582, 849
XceI RCATGY 4 cut(s) 504, 568, 1198, 1463
XcmI CCANNNNNNNNNTGG 5 cut(s) 175, 584, 671, 1045, 1619
XspI CTAG 1 cut(s) 1184
ZraI GACGTC 1 cut(s) 244
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.