Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 136 - 441 | 1.8e-63 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 966 |
| AccII | CGCG | 1 cut(s) | 588 |
| AciI | CCGC | 2 cut(s) | 194, 588 |
| AclWI | GGATC | 4 cut(s) | 147, 1200, 1235, 1249 |
| AcoI | YGGCCR | 2 cut(s) | 619, 1087 |
| AcuI | CTGAAG | 3 cut(s) | 62, 1098, 1122 |
| AfaI | GTAC | 5 cut(s) | 278, 1250, 1292, 1320, 1486 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 603, 1274, 1367, 1441 |
| AflIII | ACRYGT | 2 cut(s) | 89, 862 |
| AgsI | TTSAA | 4 cut(s) | 63, 185, 869, 1025 |
| AjiI | CACGTC | 3 cut(s) | 190, 368, 740 |
| AjnI | CCWGG | 5 cut(s) | 49, 433, 1009, 1089, 1416 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1261, 1293 |
| AluBI | AGCT | 6 cut(s) | 66, 879, 1096, 1116, 1150, 1262 |
| AluI | AGCT | 6 cut(s) | 66, 879, 1096, 1116, 1150, 1262 |
| Alw26I | GTCTC | 3 cut(s) | 51, 749, 1366 |
| AlwI | GGATC | 4 cut(s) | 147, 1200, 1235, 1249 |
| AoxI | GGCC | 3 cut(s) | 619, 1087, 1165 |
| ApeKI | GCWGC | 2 cut(s) | 857, 1411 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 794, 826 |
| AseI | ATTAAT | 1 cut(s) | 852 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1521 |
| AspS9I | GGNCC | 3 cut(s) | 362, 1007, 1358 |
| AsuC2I | CCSGG | 1 cut(s) | 100 |
| AsuHPI | GGTGA | 4 cut(s) | 808, 919, 981, 1231 |
| AvaII | GGWCC | 3 cut(s) | 362, 1007, 1358 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 511, 544 |
| BalI | TGGCCA | 2 cut(s) | 621, 1089 |
| BanII | GRGCYC | 1 cut(s) | 1447 |
| BbsI | GAAGAC | 1 cut(s) | 192 |
| BbvCI | CCTCAGC | 1 cut(s) | 390 |
| BbvI | GCAGC | 2 cut(s) | 869, 1423 |
| BccI | CCATC | 4 cut(s) | 38, 605, 686, 904 |
| BceAI | ACGGC | 1 cut(s) | 812 |
| BciT130I | CCWGG | 5 cut(s) | 51, 435, 1011, 1091, 1418 |
| BciVI | GTATCC | 1 cut(s) | 512 |
| BcnI | CCSGG | 1 cut(s) | 100 |
| BcoDI | GTCTC | 3 cut(s) | 51, 749, 1366 |
| BfaI | CTAG | 1 cut(s) | 75 |
| BfmI | CTRYAG | 1 cut(s) | 264 |
| BfuI | GTATCC | 1 cut(s) | 512 |
| BisI | GCNGC | 3 cut(s) | 194, 858, 1412 |
| BlsI | GCNGC | 3 cut(s) | 195, 859, 1413 |
| BmcAI | AGTACT | 2 cut(s) | 278, 1486 |
| Bme1390I | CCNGG | 6 cut(s) | 51, 100, 435, 1011, 1091, 1418 |
| Bme18I | GGWCC | 3 cut(s) | 362, 1007, 1358 |
| BmgBI | CACGTC | 3 cut(s) | 190, 368, 740 |
| BmgT120I | GGNCC | 3 cut(s) | 362, 1007, 1358 |
| BmiI | GGNNCC | 4 cut(s) | 595, 697, 1008, 1451 |
| BmrFI | CCNGG | 6 cut(s) | 51, 100, 435, 1011, 1091, 1418 |
| BmsI | GCATC | 2 cut(s) | 719, 1123 |
| BpiI | GAAGAC | 1 cut(s) | 192 |
| BpmI | CTGGAG | 1 cut(s) | 33 |
| Bpu10I | CCTNAGC | 1 cut(s) | 390 |
| BpuEI | CTTGAG | 1 cut(s) | 1022 |
| BpuMI | CCSGG | 1 cut(s) | 100 |
| BsaAI | YACGTR | 2 cut(s) | 90, 1248 |
| BsaI | GGTCTC | 1 cut(s) | 749 |
| BsaJI | CCNNGG | 4 cut(s) | 335, 433, 601, 1404 |
| BsaWI | WCCGGW | 1 cut(s) | 793 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 699, 729 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 603, 1274, 1367, 1441 |
| Bse3DI | GCAATG | 3 cut(s) | 239, 1071, 1335 |
| BseBI | CCWGG | 5 cut(s) | 51, 435, 1011, 1091, 1418 |
| BseDI | CCNNGG | 4 cut(s) | 335, 433, 601, 1404 |
| BseGI | GGATG | 4 cut(s) | 298, 400, 597, 1138 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 603, 1274, 1367, 1441 |
| BseMI | GCAATG | 3 cut(s) | 239, 1071, 1335 |
| BseMII | CTCAG | 2 cut(s) | 381, 1472 |
| BseRI | GAGGAG | 4 cut(s) | 702, 848, 1142, 1421 |
| BseXI | GCAGC | 2 cut(s) | 869, 1423 |
| BsgI | GTGCAG | 1 cut(s) | 1279 |
| Bsh1236I | CGCG | 1 cut(s) | 588 |
| BshFI | GGCC | 3 cut(s) | 621, 1089, 1167 |
| BsiSI | CCGG | 2 cut(s) | 99, 794 |
| BslFI | GGGAC | 2 cut(s) | 179, 619 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 603, 1274, 1367, 1441 |
| BsmAI | GTCTC | 3 cut(s) | 51, 749, 1366 |
| BsmFI | GGGAC | 2 cut(s) | 179, 619 |
| BsmI | GAATGC | 1 cut(s) | 342 |
| BsnI | GGCC | 3 cut(s) | 621, 1089, 1167 |
| Bso31I | GGTCTC | 1 cut(s) | 749 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1447 |
| Bsp1407I | TGTACA | 1 cut(s) | 1318 |
| Bsp143I | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| BspACI | CCGC | 2 cut(s) | 194, 588 |
| BspANI | GGCC | 3 cut(s) | 621, 1089, 1167 |
| BspCNI | CTCAG | 2 cut(s) | 382, 1473 |
| BspFNI | CGCG | 1 cut(s) | 588 |
| BspLI | GGNNCC | 4 cut(s) | 595, 697, 1008, 1451 |
| BspPI | GGATC | 4 cut(s) | 147, 1200, 1235, 1249 |
| BspTNI | GGTCTC | 1 cut(s) | 749 |
| BsrDI | GCAATG | 3 cut(s) | 239, 1071, 1335 |
| BsrGI | TGTACA | 1 cut(s) | 1318 |
| BssECI | CCNNGG | 4 cut(s) | 335, 433, 601, 1404 |
| BssMI | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| BssT1I | CCWWGG | 2 cut(s) | 335, 601 |
| Bst2UI | CCWGG | 5 cut(s) | 51, 435, 1011, 1091, 1418 |
| Bst4CI | ACNGT | 5 cut(s) | 301, 448, 971, 1020, 1277 |
| Bst6I | CTCTTC | 1 cut(s) | 22 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 854, 950 |
| BstAUI | TGTACA | 1 cut(s) | 1318 |
| BstBAI | YACGTR | 2 cut(s) | 90, 1248 |
| BstC8I | GCNNGC | 5 cut(s) | 357, 951, 955, 1087, 1336 |
| BstDEI | CTNAG | 3 cut(s) | 150, 390, 1481 |
| BstF5I | GGATG | 4 cut(s) | 298, 400, 597, 1138 |
| BstFNI | CGCG | 1 cut(s) | 588 |
| BstKTI | GATC | 7 cut(s) | 142, 462, 658, 1188, 1195, 1243, 1257 |
| BstMAI | GTCTC | 3 cut(s) | 51, 749, 1366 |
| BstMBI | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 845, 854, 941, 950, 1082, 1313 |
| BstNI | CCWGG | 5 cut(s) | 51, 435, 1011, 1091, 1418 |
| BstNSI | RCATGY | 1 cut(s) | 866 |
| BstSCI | CCNGG | 6 cut(s) | 49, 98, 433, 1009, 1089, 1416 |
| BstSFI | CTRYAG | 1 cut(s) | 264 |
| BstSNI | TACGTA | 1 cut(s) | 1248 |
| BstUI | CGCG | 1 cut(s) | 588 |
| BstV1I | GCAGC | 2 cut(s) | 869, 1423 |
| BstV2I | GAAGAC | 1 cut(s) | 192 |
| BstX2I | RGATCY | 1 cut(s) | 1240 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 485, 1163 |
| BstYI | RGATCY | 1 cut(s) | 1240 |
| BsuI | GTATCC | 1 cut(s) | 512 |
| BsuRI | GGCC | 3 cut(s) | 621, 1089, 1167 |
| BtrI | CACGTC | 3 cut(s) | 190, 368, 740 |
| BtsCI | GGATG | 4 cut(s) | 298, 400, 597, 1138 |
| BtsIMutI | CAGTG | 1 cut(s) | 444 |
| Cac8I | GCNNGC | 5 cut(s) | 357, 951, 955, 1087, 1336 |
| Cfr13I | GGNCC | 3 cut(s) | 362, 1007, 1358 |
| CsiI | ACCWGGT | 1 cut(s) | 1416 |
| Csp6I | GTAC | 5 cut(s) | 277, 1249, 1291, 1319, 1485 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 571, 606 |
| CviAII | CATG | 7 cut(s) | 136, 401, 623, 863, 1060, 1252, 1322 |
| CviQI | GTAC | 5 cut(s) | 277, 1249, 1291, 1319, 1485 |
| DdeI | CTNAG | 3 cut(s) | 150, 390, 1481 |
| DpnI | GATC | 7 cut(s) | 141, 461, 657, 1187, 1194, 1242, 1256 |
| DpnII | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| EaeI | YGGCCR | 2 cut(s) | 619, 1087 |
| Eam1104I | CTCTTC | 1 cut(s) | 22 |
| EarI | CTCTTC | 1 cut(s) | 22 |
| Eco105I | TACGTA | 1 cut(s) | 1248 |
| Eco130I | CCWWGG | 2 cut(s) | 335, 601 |
| Eco24I | GRGCYC | 1 cut(s) | 1447 |
| Eco31I | GGTCTC | 1 cut(s) | 749 |
| Eco32I | GATATC | 1 cut(s) | 577 |
| Eco47I | GGWCC | 3 cut(s) | 362, 1007, 1358 |
| Eco57I | CTGAAG | 3 cut(s) | 62, 1098, 1122 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1007, 1358 |
| EcoRII | CCWGG | 5 cut(s) | 49, 433, 1009, 1089, 1416 |
| EcoRV | GATATC | 1 cut(s) | 577 |
| EcoT14I | CCWWGG | 2 cut(s) | 335, 601 |
| EcoT38I | GRGCYC | 1 cut(s) | 1447 |
| ErhI | CCWWGG | 2 cut(s) | 335, 601 |
| FaeI | CATG | 7 cut(s) | 139, 404, 626, 866, 1063, 1255, 1325 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 263, 295, 1181, 1213 |
| FaqI | GGGAC | 2 cut(s) | 179, 619 |
| FatI | CATG | 7 cut(s) | 135, 400, 622, 862, 1059, 1251, 1321 |
| FauNDI | CATATG | 1 cut(s) | 480 |
| FblI | GTMKAC | 1 cut(s) | 966 |
| Fnu4HI | GCNGC | 3 cut(s) | 194, 858, 1412 |
| FokI | GGATG | 4 cut(s) | 305, 407, 584, 1145 |
| FriOI | GRGCYC | 1 cut(s) | 1447 |
| Fsp4HI | GCNGC | 3 cut(s) | 194, 858, 1412 |
| FspBI | CTAG | 1 cut(s) | 75 |
| GluI | GCNGC | 3 cut(s) | 194, 858, 1412 |
| GsuI | CTGGAG | 1 cut(s) | 33 |
| HaeIII | GGCC | 3 cut(s) | 621, 1089, 1167 |
| HapII | CCGG | 2 cut(s) | 99, 794 |
| Hin1II | CATG | 7 cut(s) | 139, 404, 626, 866, 1063, 1255, 1325 |
| HindIII | AAGCTT | 1 cut(s) | 877 |
| HpaII | CCGG | 2 cut(s) | 99, 794 |
| HphI | GGTGA | 4 cut(s) | 808, 919, 981, 1231 |
| Hpy166II | GTNNAC | 5 cut(s) | 87, 676, 967, 1016, 1291 |
| Hpy188I | TCNGA | 9 cut(s) | 42, 58, 205, 225, 874, 1105, 1259, 1282, 1543 |
| Hpy188III | TCNNGA | 9 cut(s) | 182, 412, 537, 983, 1001, 1140, 1201, 1480, 1529 |
| Hpy8I | GTNNAC | 5 cut(s) | 87, 676, 967, 1016, 1291 |
| HpyAV | CCTTC | 3 cut(s) | 697, 898, 1073 |
| HpyCH4III | ACNGT | 5 cut(s) | 301, 448, 971, 1020, 1277 |
| HpyCH4IV | ACGT | 7 cut(s) | 89, 177, 189, 367, 739, 844, 1247 |
| HpyCH4V | TGCA | 7 cut(s) | 244, 475, 848, 857, 944, 953, 1296 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 845, 854, 941, 950, 1082, 1313 |
| HpyF3I | CTNAG | 3 cut(s) | 150, 390, 1481 |
| HpySE526I | ACGT | 7 cut(s) | 89, 177, 189, 367, 739, 844, 1247 |
| Hsp92II | CATG | 7 cut(s) | 139, 404, 626, 866, 1063, 1255, 1325 |
| Kzo9I | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| LmnI | GCTCC | 6 cut(s) | 707, 1093, 1155, 1325, 1408, 1455 |
| Lsp1109I | GCAGC | 2 cut(s) | 869, 1423 |
| LweI | GCATC | 2 cut(s) | 719, 1123 |
| MabI | ACCWGGT | 1 cut(s) | 1416 |
| MaeI | CTAG | 1 cut(s) | 75 |
| MaeII | ACGT | 7 cut(s) | 89, 177, 189, 367, 739, 844, 1247 |
| MaeIII | GTNAC | 3 cut(s) | 320, 412, 1055 |
| MalI | GATC | 7 cut(s) | 141, 461, 657, 1187, 1194, 1242, 1256 |
| MboI | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| MboII | GAAGA | 7 cut(s) | 9, 12, 197, 212, 1195, 1449, 1529 |
| MflI | RGATCY | 1 cut(s) | 1240 |
| MhlI | GDGCHC | 1 cut(s) | 1447 |
| MlsI | TGGCCA | 2 cut(s) | 621, 1089 |
| MluCI | AATT | 6 cut(s) | 546, 681, 729, 849, 886, 1582 |
| MluNI | TGGCCA | 2 cut(s) | 621, 1089 |
| MlyI | GAGTC | 4 cut(s) | 432, 550, 815, 1035 |
| MmeI | TCCRAC | 1 cut(s) | 248 |
| Mox20I | TGGCCA | 2 cut(s) | 621, 1089 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1521 |
| MscI | TGGCCA | 2 cut(s) | 621, 1089 |
| MseI | TTAA | 4 cut(s) | 285, 651, 732, 852 |
| MslI | CAYNNNNRTG | 2 cut(s) | 483, 794 |
| Msp20I | TGGCCA | 2 cut(s) | 621, 1089 |
| MspI | CCGG | 2 cut(s) | 99, 794 |
| MspR9I | CCNGG | 6 cut(s) | 51, 100, 435, 1011, 1091, 1418 |
| Mva1269I | GAATGC | 1 cut(s) | 342 |
| MvaI | CCWGG | 5 cut(s) | 51, 435, 1011, 1091, 1418 |
| MvnI | CGCG | 1 cut(s) | 588 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 845, 854, 941, 950, 1082, 1313 |
| NciI | CCSGG | 1 cut(s) | 100 |
| NdeI | CATATG | 1 cut(s) | 480 |
| NdeII | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| NlaIII | CATG | 7 cut(s) | 139, 404, 626, 866, 1063, 1255, 1325 |
| NlaIV | GGNNCC | 4 cut(s) | 595, 697, 1008, 1451 |
| NmeAIII | GCCGAG | 2 cut(s) | 983, 1426 |
| NmuCI | GTSAC | 3 cut(s) | 320, 412, 1055 |
| NspI | RCATGY | 1 cut(s) | 866 |
| PciI | ACATGT | 1 cut(s) | 862 |
| PctI | GAATGC | 1 cut(s) | 342 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1521 |
| PfeI | GAWTC | 6 cut(s) | 59, 663, 765, 869, 979, 986 |
| PflFI | GACNNNGTC | 2 cut(s) | 366, 741 |
| PkrI | GCNGC | 3 cut(s) | 195, 859, 1413 |
| PleI | GAGTC | 4 cut(s) | 432, 550, 815, 1035 |
| PpsI | GAGTC | 4 cut(s) | 432, 550, 815, 1035 |
| Ppu21I | YACGTR | 2 cut(s) | 90, 1248 |
| PpuMI | RGGWCCY | 2 cut(s) | 1007, 1358 |
| PscI | ACATGT | 1 cut(s) | 862 |
| PshBI | ATTAAT | 1 cut(s) | 852 |
| Psp5II | RGGWCCY | 2 cut(s) | 1007, 1358 |
| Psp6I | CCWGG | 5 cut(s) | 49, 433, 1009, 1089, 1416 |
| PspGI | CCWGG | 5 cut(s) | 49, 433, 1009, 1089, 1416 |
| PspN4I | GGNNCC | 4 cut(s) | 595, 697, 1008, 1451 |
| PspPI | GGNCC | 3 cut(s) | 362, 1007, 1358 |
| PspPPI | RGGWCCY | 2 cut(s) | 1007, 1358 |
| PsuI | RGATCY | 1 cut(s) | 1240 |
| PsyI | GACNNNGTC | 2 cut(s) | 366, 741 |
| RsaI | GTAC | 5 cut(s) | 278, 1250, 1292, 1320, 1486 |
| RsaNI | GTAC | 5 cut(s) | 277, 1249, 1291, 1319, 1485 |
| RseI | CAYNNNNRTG | 2 cut(s) | 483, 794 |
| SaqAI | TTAA | 4 cut(s) | 285, 651, 732, 852 |
| SatI | GCNGC | 3 cut(s) | 194, 858, 1412 |
| Sau3AI | GATC | 7 cut(s) | 139, 459, 655, 1185, 1192, 1240, 1254 |
| Sau96I | GGNCC | 3 cut(s) | 362, 1007, 1358 |
| ScaI | AGTACT | 2 cut(s) | 278, 1486 |
| SchI | GAGTC | 4 cut(s) | 432, 550, 815, 1035 |
| ScrFI | CCNGG | 6 cut(s) | 51, 100, 435, 1011, 1091, 1418 |
| SduI | GDGCHC | 1 cut(s) | 1447 |
| SexAI | ACCWGGT | 1 cut(s) | 1416 |
| SfaNI | GCATC | 2 cut(s) | 719, 1123 |
| SfcI | CTRYAG | 1 cut(s) | 264 |
| SinI | GGWCC | 3 cut(s) | 362, 1007, 1358 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 483, 794 |
| SmlI | CTYRAG | 1 cut(s) | 1001 |
| SmoI | CTYRAG | 1 cut(s) | 1001 |
| SnaBI | TACGTA | 1 cut(s) | 1248 |
| Sse9I | AATT | 6 cut(s) | 546, 681, 729, 849, 886, 1582 |
| SsiI | CCGC | 2 cut(s) | 194, 588 |
| SspMI | CTAG | 1 cut(s) | 75 |
| StyD4I | CCNGG | 6 cut(s) | 49, 98, 433, 1009, 1089, 1416 |
| StyI | CCWWGG | 2 cut(s) | 335, 601 |
| TaaI | ACNGT | 5 cut(s) | 301, 448, 971, 1020, 1277 |
| TaiI | ACGT | 7 cut(s) | 92, 180, 192, 370, 742, 847, 1250 |
| TaqI | TCGA | 3 cut(s) | 538, 658, 1202 |
| TaqII | GACCGA | 1 cut(s) | 1449 |
| TasI | AATT | 6 cut(s) | 546, 681, 729, 849, 886, 1582 |
| TatI | WGTACW | 4 cut(s) | 276, 1290, 1318, 1484 |
| TauI | GCSGC | 1 cut(s) | 196 |
| TfiI | GAWTC | 6 cut(s) | 59, 663, 765, 869, 979, 986 |
| Tru1I | TTAA | 4 cut(s) | 285, 651, 732, 852 |
| Tru9I | TTAA | 4 cut(s) | 285, 651, 732, 852 |
| TscAI | CASTG | 1 cut(s) | 451 |
| TseFI | GTSAC | 3 cut(s) | 320, 412, 1055 |
| TseI | GCWGC | 2 cut(s) | 857, 1411 |
| Tsp45I | GTSAC | 3 cut(s) | 320, 412, 1055 |
| TspDTI | ATGAA | 5 cut(s) | 335, 389, 1382, 1514, 1530 |
| TspRI | CASTG | 1 cut(s) | 451 |
| Tth111I | GACNNNGTC | 2 cut(s) | 366, 741 |
| VpaK11BI | GGWCC | 3 cut(s) | 362, 1007, 1358 |
| VspI | ATTAAT | 1 cut(s) | 852 |
| XceI | RCATGY | 1 cut(s) | 866 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 314, 587 |
| XmiI | GTMKAC | 1 cut(s) | 966 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1521 |
| XspI | CTAG | 1 cut(s) | 75 |
| ZrmI | AGTACT | 2 cut(s) | 278, 1486 |