Rh2CG404200

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000762
Physical Location & Seq
Forward (+)
55054394 .. 55059882
5489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG404200.1

Sequence Viewer

Length: 1626 bp
ATGGCCAACTCTGCTTCTTCCATTTCCACCACCACTCCACGGTTCCGCTGCTTCGACCCCACCAACTCTTACGCTGCGACGCCCGATCACCCCCGCCGCTTCAGCACCACCTCCAATTCAACTCCCAGGTACTACAACAACAACAACAACTCCAAGGGCACTATGGGAATTTCCGCCAACTCCGAGCCCAAGATCATCACCGGCGCCGCTGGCTACGTTCTCGAAGACGTTCCTCACTTGTCCGATTACATACCCGATCTCCCTACATATCCCAATCCATTACAGGACAATCCTTCCTATTCAGTTGTTAAGCAGTATTTCGTTAATGTCGATGATAGTGTTGCCCAGACGATTGTAGTCCAGAAAGATAGTCCAAGAGGCACACATTTTCGACGTGCTGGACCACGTCAGAAGGTCTATTTTGAACCAGATGATGTTCGTGCGTGTATTGTGACATGTGGTGGTTTATGTCCAGGCTTAAATACAGTAATTAGAGAAATAGTATGTGGGTTGTACTACATGTATGGTGTCCACCGAATTCTTGGAATAGAGGGAGGATACAGAGGGTTTTATGCCAGAAATACAATTCCCTTAACACCAAAGGTTGTGAATGACATCCATAAACGTGGTGGCACCATCCTTGGGACATCACGAGGTGGCCATGATACCTCAAAGATTGTGGACAGCATTCAGGACAGAGGAATTAATCAGGTTTACATACTTGGGGGAGACGGTACACAGAAAGGAGCTTCTGTAATATTTGAGGAATGCAGGAGGCGTGGACTCAATGTTGCAGTGGCAGGAATTCCAAAAACCATTGATAATGATATTCCGGTTATAGACAAGTCCTTTGGTTTTGACACTGCTGTTGAGGAGGCTCAACGAGCCATTAATGCAGCTCATGTTGAAGCAGAAAGCATTGAGAATGGTATCGGCGTTGTCAAGTTAATGGGTCGCTACAGTGGATTTATAGCTATGTATGCTACTCTTGCCAGCCGAGATGTGGATTGTTGCCTGATTCCAGAGTCGCCTTTCTATCTGGAAGGAGCAGGTGGATTGTTTGAGTACATTGAAAAAAGACTTAAAGAGAACGGCCACATGGTTATAGTAATAGCTGAAGGCGCAGAACAAGAGCTTCTAGCTGAAAGCAGGCTTGCTATGAATCATCAGGACGCTTCTGGAAATAAGCTTCTTCAAGATTCTGGTCTGTGGTTATCGCAGAAGATTAAGGACCACTTCTCAAAACTGAACAAGATGTCTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCTATTCCAAGCAATGCATCTGATAATGTATACTGCACCCTCCTTGCTCATAGTGTGGTTCATGGTGCAATGGCAGGATACACCGGCTTCACCGTCGGCTCTGTTAATGGAAGGCATGCTTATATTCCTTTCCATCGGGTGACGGAGAGACAGAACAAGGTTGTGATTACTGACAGAATGTGGGCACGGCTATTATCATCAACTAATCAGCCAAGTTTCTTGGATCCGAAGGAAGTAGCTAAAAAGAAAGAGGAAGAAACAGAAACCCACCTGGTCAATGGGGAAAACCATGTGGAGGAAACTGCTTCGGCTACGCCAAAGGAGTAG

Protein Analysis

541

Amino Acids

59.55

Weight (kDa)

6.61

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 148 - 454 9.7e-63 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1040
Acc36I ACCTGC 1 cut(s) 1040
AccB1I GGYRCC 2 cut(s) 203, 632
AccI GTMKAC 1 cut(s) 1329
AciI CCGC 5 cut(s) 46, 94, 97, 174, 207
AclWI GGATC 4 cut(s) 1274, 1288, 1517, 1530
AcoI YGGCCR 3 cut(s) 3, 658, 1093
AcsI RAATTY 3 cut(s) 168, 537, 804
AcuI CTGAAG 2 cut(s) 85, 1137
AcyI GRCGYC 2 cut(s) 80, 204
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 4 cut(s) 131, 515, 736, 1067
AfiI CCNNNNNNNGG 4 cut(s) 39, 642, 1003, 1594
AflIII ACRYGT 2 cut(s) 455, 519
AgsI TTSAA 5 cut(s) 120, 425, 908, 1073, 1196
AjiI CACGTC 2 cut(s) 395, 407
AjnI CCWGG 3 cut(s) 125, 472, 1569
AluBI AGCT 8 cut(s) 749, 899, 974, 1115, 1135, 1142, 1189, 1538
AluI AGCT 8 cut(s) 749, 899, 974, 1115, 1135, 1142, 1189, 1538
Alw26I GTCTC 2 cut(s) 723, 1441
AlwI GGATC 4 cut(s) 1274, 1288, 1517, 1530
AoxI GGCC 3 cut(s) 3, 658, 1093
ApeKI GCWGC 3 cut(s) 48, 74, 896
ApoI RAATTY 3 cut(s) 168, 537, 804
ArsI GACNNNNNNTTYG 2 cut(s) 833, 865
AseI ATTAAT 2 cut(s) 705, 891
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 2 cut(s) 206, 1124
AspS9I GGNCC 2 cut(s) 401, 1231
AsuHPI GGTGA 4 cut(s) 80, 190, 1381, 1450
AvaII GGWCC 2 cut(s) 401, 1231
BaeGI GKGCMC 2 cut(s) 161, 1486
BalI TGGCCA 2 cut(s) 5, 660
BamHI GGATCC 1 cut(s) 1522
BanI GGYRCC 2 cut(s) 203, 632
BanII GRGCYC 1 cut(s) 189
BauI CACGAG 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 35, 61, 908
BccI CCATC 2 cut(s) 644, 1440
BceAI ACGGC 2 cut(s) 1108, 1502
BciT130I CCWGG 3 cut(s) 127, 474, 1571
BciVI GTATCC 2 cut(s) 551, 1370
BcoDI GTCTC 2 cut(s) 723, 1441
BfaI CTAG 1 cut(s) 1139
BfmI CTRYAG 1 cut(s) 958
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 1040
BfuI GTATCC 2 cut(s) 551, 1370
BisI GCNGC 5 cut(s) 49, 75, 97, 207, 897
BlsI GCNGC 5 cut(s) 50, 76, 98, 208, 898
Bme1390I CCNGG 3 cut(s) 127, 474, 1571
Bme18I GGWCC 2 cut(s) 401, 1231
BmgBI CACGTC 2 cut(s) 395, 407
BmgT120I GGNCC 2 cut(s) 401, 1231
BmiI GGNNCC 4 cut(s) 44, 205, 634, 1524
BmrFI CCNGG 3 cut(s) 127, 474, 1571
BmsI GCATC 1 cut(s) 1325
BpiI GAAGAC 1 cut(s) 231
BsaHI GRCGYC 2 cut(s) 80, 204
BsaJI CCNNGG 4 cut(s) 38, 125, 153, 640
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 8 cut(s) 19, 49, 134, 164, 243, 273, 738, 768
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 642, 1003, 1594
Bse118I RCCGGY 2 cut(s) 200, 1382
Bse3DI GCAATG 2 cut(s) 1318, 1374
BseBI CCWGG 3 cut(s) 127, 474, 1571
BseDI CCNNGG 4 cut(s) 38, 125, 153, 640
BseGI GGATG 2 cut(s) 615, 636
BseLI CCNNNNNNNGG 4 cut(s) 39, 642, 1003, 1594
BseMI GCAATG 2 cut(s) 1318, 1374
BseRI GAGGAG 1 cut(s) 887
BseSI GKGCMC 2 cut(s) 161, 1486
BseXI GCAGC 3 cut(s) 35, 61, 908
BsgI GTGCAG 1 cut(s) 1318
BshFI GGCC 3 cut(s) 5, 660, 1095
BshNI GGYRCC 2 cut(s) 203, 632
BsiSI CCGG 3 cut(s) 201, 833, 1383
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 4 cut(s) 39, 642, 1003, 1594
BsmAI GTCTC 2 cut(s) 723, 1441
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 2 cut(s) 687, 773
BsnI GGCC 3 cut(s) 5, 660, 1095
Bsp1286I GDGCHC 3 cut(s) 161, 189, 1486
Bsp143I GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
BspACI CCGC 5 cut(s) 46, 94, 97, 174, 207
BspANI GGCC 3 cut(s) 5, 660, 1095
BspLI GGNNCC 4 cut(s) 44, 205, 634, 1524
BspMI ACCTGC 1 cut(s) 1040
BspPI GGATC 4 cut(s) 1274, 1288, 1517, 1530
BspT107I GGYRCC 2 cut(s) 203, 632
BsrDI GCAATG 2 cut(s) 1318, 1374
BsrFI RCCGGY 2 cut(s) 200, 1382
BssAI RCCGGY 2 cut(s) 200, 1382
BssECI CCNNGG 4 cut(s) 38, 125, 153, 640
BssMI GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
BssNAI GTATAC 1 cut(s) 1330
BssNI GRCGYC 2 cut(s) 80, 204
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 2 cut(s) 153, 640
Bst1107I GTATAC 1 cut(s) 1330
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 3 cut(s) 127, 474, 1571
Bst4CI ACNGT 5 cut(s) 42, 487, 734, 962, 1393
BstACI GRCGYC 2 cut(s) 80, 204
BstC8I GCNNGC 5 cut(s) 211, 994, 1151, 1155, 1416
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 615, 636
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 206, 1124
BstKTI GATC 6 cut(s) 88, 195, 259, 1282, 1296, 1525
BstMAI GTCTC 2 cut(s) 723, 1441
BstMBI GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
BstMWI GCNNNNNNNGC 8 cut(s) 11, 102, 210, 884, 893, 980, 989, 1121
BstNI CCWGG 3 cut(s) 127, 474, 1571
BstNSI RCATGY 3 cut(s) 459, 523, 1418
BstSCI CCNGG 3 cut(s) 125, 472, 1569
BstSFI CTRYAG 1 cut(s) 958
BstSLI GKGCMC 2 cut(s) 161, 1486
BstV1I GCAGC 3 cut(s) 35, 61, 908
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 2 cut(s) 1279, 1522
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 2 cut(s) 1279, 1522
BstZ17I GTATAC 1 cut(s) 1330
BsuI GTATCC 2 cut(s) 551, 1370
BsuRI GGCC 3 cut(s) 5, 660, 1095
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 2 cut(s) 395, 407
BtsCI GGATG 2 cut(s) 615, 636
BtsI GCAGTG 2 cut(s) 801, 861
BtsIMutI CAGTG 3 cut(s) 801, 861, 967
BveI ACCTGC 1 cut(s) 1040
Cac8I GCNNGC 5 cut(s) 211, 994, 1151, 1155, 1416
CfoI GCGC 2 cut(s) 206, 1124
Cfr10I RCCGGY 2 cut(s) 200, 1382
Cfr13I GGNCC 2 cut(s) 401, 1231
CseI GACGC 2 cut(s) 88, 1181
CsiI ACCWGGT 1 cut(s) 1569
Csp6I GTAC 4 cut(s) 130, 514, 735, 1066
CspCI CAANNNNNGTGG 2 cut(s) 660, 695
CviAII CATG 9 cut(s) 456, 520, 662, 902, 1099, 1291, 1361, 1415, 1589
CviQI GTAC 4 cut(s) 130, 514, 735, 1066
DinI GGCGCC 1 cut(s) 205
DpnI GATC 6 cut(s) 87, 194, 258, 1281, 1295, 1524
DpnII GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
DraIII CACNNNGTG 1 cut(s) 656
EaeI YGGCCR 3 cut(s) 3, 658, 1093
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 153, 640
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 401, 1231
Eco57I CTGAAG 2 cut(s) 85, 1137
EcoRI GAATTC 2 cut(s) 537, 804
EcoRII CCWGG 3 cut(s) 125, 472, 1569
EcoT14I CCWWGG 2 cut(s) 153, 640
EcoT22I ATGCAT 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 189
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 153, 640
Esp3I CGTCTC 1 cut(s) 723
FaeI CATG 9 cut(s) 459, 523, 665, 905, 1102, 1294, 1364, 1418, 1592
FalI AAGNNNNNCTT 4 cut(s) 1220, 1252, 1402, 1434
FaqI GGGAC 1 cut(s) 658
FatI CATG 9 cut(s) 455, 519, 661, 901, 1098, 1290, 1360, 1414, 1588
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 1 cut(s) 1329
Fnu4HI GCNGC 5 cut(s) 49, 75, 97, 207, 897
FokI GGATG 2 cut(s) 602, 623
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 5 cut(s) 49, 75, 97, 207, 897
FspBI CTAG 1 cut(s) 1139
GlaI GCGC 2 cut(s) 205, 1123
GluI GCNGC 5 cut(s) 49, 75, 97, 207, 897
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 3 cut(s) 5, 660, 1095
HapII CCGG 3 cut(s) 201, 833, 1383
HgaI GACGC 2 cut(s) 88, 1181
HhaI GCGC 2 cut(s) 206, 1124
Hin1I GRCGYC 2 cut(s) 80, 204
Hin1II CATG 9 cut(s) 459, 523, 665, 905, 1102, 1294, 1364, 1418, 1592
Hin6I GCGC 2 cut(s) 204, 1122
HinP1I GCGC 2 cut(s) 204, 1122
HindIII AAGCTT 1 cut(s) 1187
HinfI GANTC 5 cut(s) 783, 1018, 1025, 1162, 1199
HpaII CCGG 3 cut(s) 201, 833, 1383
HphI GGTGA 4 cut(s) 80, 190, 1381, 1450
Hpy166II GTNNAC 6 cut(s) 532, 682, 715, 737, 782, 1330
Hpy188I TCNGA 5 cut(s) 184, 244, 411, 1321, 1527
Hpy188III TCNNGA 9 cut(s) 221, 361, 651, 692, 1022, 1040, 1169, 1179, 1196
Hpy8I GTNNAC 6 cut(s) 532, 682, 715, 737, 782, 1330
Hpy99I CGWCG 3 cut(s) 82, 396, 1397
HpyAV CCTTC 6 cut(s) 303, 406, 1037, 1112, 1404, 1522
HpyCH4III ACNGT 5 cut(s) 42, 487, 734, 962, 1393
HpyCH4IV ACGT 5 cut(s) 216, 228, 394, 406, 625
HpyCH4V TGCA 6 cut(s) 771, 794, 896, 1316, 1335, 1367
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 102, 210, 884, 893, 980, 989, 1121
HpySE526I ACGT 5 cut(s) 216, 228, 394, 406, 625
Hsp92I GRCGYC 2 cut(s) 80, 204
Hsp92II CATG 9 cut(s) 459, 523, 665, 905, 1102, 1294, 1364, 1418, 1592
HspAI GCGC 2 cut(s) 204, 1122
KasI GGCGCC 1 cut(s) 203
Kzo9I GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
LmnI GCTCC 2 cut(s) 746, 1046
Lsp1109I GCAGC 3 cut(s) 35, 61, 908
LweI GCATC 1 cut(s) 1325
MabI ACCWGGT 1 cut(s) 1569
MaeI CTAG 1 cut(s) 1139
MaeII ACGT 5 cut(s) 216, 228, 394, 406, 625
MaeIII GTNAC 2 cut(s) 451, 1438
MalI GATC 6 cut(s) 87, 194, 258, 1281, 1295, 1524
MboI GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
MboII GAAGA 5 cut(s) 9, 236, 1184, 1234, 1565
MflI RGATCY 2 cut(s) 1279, 1522
MhlI GDGCHC 3 cut(s) 161, 189, 1486
MlsI TGGCCA 2 cut(s) 5, 660
MluCI AATT 7 cut(s) 115, 168, 489, 537, 585, 702, 804
MluNI TGGCCA 2 cut(s) 5, 660
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 777, 1034
Mox20I TGGCCA 2 cut(s) 5, 660
Mph1103I ATGCAT 1 cut(s) 1318
MroXI GAANNNNTTC 1 cut(s) 228
MscI TGGCCA 2 cut(s) 5, 660
MslI CAYNNNNRTG 1 cut(s) 624
Msp20I TGGCCA 2 cut(s) 5, 660
MspA1I CMGCKG 2 cut(s) 48, 209
MspI CCGG 3 cut(s) 201, 833, 1383
MspR9I CCNGG 3 cut(s) 127, 474, 1571
Mva1269I GAATGC 2 cut(s) 687, 773
MvaI CCWGG 3 cut(s) 127, 474, 1571
MwoI GCNNNNNNNGC 8 cut(s) 11, 102, 210, 884, 893, 980, 989, 1121
NarI GGCGCC 1 cut(s) 204
NdeII GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
NlaIII CATG 9 cut(s) 459, 523, 665, 905, 1102, 1294, 1364, 1418, 1592
NlaIV GGNNCC 4 cut(s) 44, 205, 634, 1524
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 2 cut(s) 451, 1438
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 3 cut(s) 459, 523, 1418
PaeI GCATGC 1 cut(s) 1418
PaqCI CACCTGC 1 cut(s) 1040
PciI ACATGT 2 cut(s) 455, 519
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 2 cut(s) 687, 773
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 3 cut(s) 1018, 1162, 1199
PflFI GACNNNGTC 1 cut(s) 405
PkrI GCNGC 5 cut(s) 50, 76, 98, 208, 898
PleI GAGTC 2 cut(s) 777, 1033
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 2 cut(s) 777, 1033
PscI ACATGT 2 cut(s) 455, 519
PshBI ATTAAT 2 cut(s) 705, 891
Psp6I CCWGG 3 cut(s) 125, 472, 1569
PspGI CCWGG 3 cut(s) 125, 472, 1569
PspN4I GGNNCC 4 cut(s) 44, 205, 634, 1524
PspPI GGNCC 2 cut(s) 401, 1231
PsuI RGATCY 2 cut(s) 1279, 1522
PsyI GACNNNGTC 1 cut(s) 405
RsaI GTAC 4 cut(s) 131, 515, 736, 1067
RsaNI GTAC 4 cut(s) 130, 514, 735, 1066
RseI CAYNNNNRTG 1 cut(s) 624
SatI GCNGC 5 cut(s) 49, 75, 97, 207, 897
Sau3AI GATC 6 cut(s) 85, 192, 256, 1279, 1293, 1522
Sau96I GGNCC 2 cut(s) 401, 1231
SchI GAGTC 2 cut(s) 777, 1034
ScrFI CCNGG 3 cut(s) 127, 474, 1571
SduI GDGCHC 3 cut(s) 161, 189, 1486
SexAI ACCWGGT 1 cut(s) 1569
SfaNI GCATC 1 cut(s) 1325
SfcI CTRYAG 1 cut(s) 958
SfoI GGCGCC 1 cut(s) 205
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 2 cut(s) 401, 1231
SmiMI CAYNNNNRTG 1 cut(s) 624
SphI GCATGC 1 cut(s) 1418
Sse9I AATT 7 cut(s) 115, 168, 489, 537, 585, 702, 804
SsiI CCGC 5 cut(s) 46, 94, 97, 174, 207
SspDI GGCGCC 1 cut(s) 203
SspI AATATT 1 cut(s) 759
SspMI CTAG 1 cut(s) 1139
StyD4I CCNGG 3 cut(s) 125, 472, 1569
StyI CCWWGG 2 cut(s) 153, 640
TaaI ACNGT 5 cut(s) 42, 487, 734, 962, 1393
TaiI ACGT 5 cut(s) 219, 231, 397, 409, 628
TaqI TCGA 4 cut(s) 54, 222, 330, 391
TasI AATT 7 cut(s) 115, 168, 489, 537, 585, 702, 804
TatI WGTACW 2 cut(s) 513, 1065
TauI GCSGC 2 cut(s) 99, 209
TfiI GAWTC 3 cut(s) 1018, 1162, 1199
TscAI CASTG 3 cut(s) 801, 868, 967
TseFI GTSAC 2 cut(s) 451, 1438
TseI GCWGC 3 cut(s) 48, 74, 896
Tsp45I GTSAC 2 cut(s) 451, 1438
TspDTI ATGAA 2 cut(s) 1175, 1349
TspGWI ACGGA 2 cut(s) 1286, 1457
TspRI CASTG 3 cut(s) 801, 868, 967
Tth111I GACNNNGTC 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 401, 1231
VspI ATTAAT 2 cut(s) 705, 891
XapI RAATTY 3 cut(s) 168, 537, 804
XceI RCATGY 3 cut(s) 459, 523, 1418
XcmI CCANNNNNNNNNTGG 5 cut(s) 160, 539, 626, 1000, 1574
XmiI GTMKAC 1 cut(s) 1329
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 1 cut(s) 1139
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.