MD15G1089400.v1.1

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000762
Physical Location & Seq
Reverse (-)
6220463 .. 6224279
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1089400.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGGCGATTCGAATCCAGCCGGAAACGTGCAGATGAAGGTGGTCAAGGGCGACTTCGGCTACGTTCTCGAAGACGTCCCCCATCTCTCCGATTACTTGTCCGATCTTCCTACTTATCCCAATCCGTTGCGATTCAATCCTGCTTATTCAGTTGTAAAGCAGTATTTTGTTAACGTGGATGACACCGTTGCTCAAAAGATTGTTGTTCACACGGATAGTCCGAGAGGGACACACTTCCGGCGGGCAGGACCACGCCAGAAGGTTTATTTCAACCCGGCTGAAGTACATGCTTGTATTGTAACATGTGGTGGTTTGTGCCCGGGGCTCAACACGGTTATCAGGGAAATTGTGTGTGGACTTTATCACATGTATGGTGTCTCGAAAGTACTTGGGATAGACGGAGGATACAAGGGTTTCTATGCCAGAAACACCGTTACCTTGACACCCAAGGTTGTCAATGACATCCACAAGCGTGGTGGTACCATCATTGGTACGTCGCGAGGAGGACATGATACTGCAAAGATAGTTGACAGCATTCAGGATCGTGGAATTAATCATGTTTACATAATTGGAGGTGATGGAACTCAAAAAGGAGCAGCTGTCATATATGAGGAAATTAGGCGGCGTGGCCTCAAAGTTTCTGTTGCAGGAATTCCAAAAACCATAGACAATGACATTCCGGTTATAGACAGATCTTTTGGCTTTGATACTGCGGTTGAGGAGGCTCAACGTGCCATTAATTCAGCTCATGTTGAAGCTGAAAGTATTGAGAATGGTATTGGTGTTGTGAAGCTAATGGGACGCTACAGTGGTTTCATAGCTATGTATGCTACACTTGCCAGTCGAGATGTCGACTGTTGTTTGATTCCGGAGTCCCCCTTCTATCTTGAAGGAAAAGGTGGACTTTTTGAGTACATTGAAAAACGACTCAAAGAAAATGGACATATGGTTATTGTGATAGCTGAGGGTGCAGGACAAGATCTTCTGTCAGAGAGCTTGCAATCTATGAACCAGCAGGATGCTTCAGGAAACAAGCAACTTAAAGATGTTGGGTTATGGATTTCTGAGAGGATAAAGAGTCACTTTGGAAGGCAGCAAAAGCTCGCCATTAATCTTAAGTATATAGATCCTACGTACATGATCCGGGCTATTCCAAGCAATGCAGCCGACAACGTCTACTGCACACTCCTTGCTCAAAGTGCGGTTCATGGAGCAATGGCAGGGTACACGGGCTTTATATGTGGGCCTGTCAATGGAAGGCATTCTTATATTCCTTTCAATCATATTACAGAGAAGCAGAACAAGGTTGTGATTACTGACAGGATGTGGGCGCGACTCCTTTCTTCAACCAACCAGCCGAGCTTCTTGAACCCCAAAGATGTCACCGAAATCCAAGATGAAGATCCTCCAACCCAGATGCTGGATGGGGAAAACTGTAAAGATAGCAAGTTCGCAACAAAAGACATCCCAAGCATGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

494

Amino Acids

54.14

Weight (kDa)

6.71

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 98 - 403 3.1e-63 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
Acc65I GGTACC 1 cut(s) 479
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 1420
AccI GTMKAC 2 cut(s) 852, 1176
AccII CGCG 2 cut(s) 499, 1333
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 4 cut(s) 241, 622, 713, 1202
AclWI GGATC 4 cut(s) 549, 1121, 1135, 1397
AcsI RAATTY 1 cut(s) 651
AcuI CTGAAG 2 cut(s) 300, 1008
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 7 cut(s) 285, 387, 481, 493, 914, 1136, 1226
AfiI CCNNNNNNNGG 2 cut(s) 1253, 1420
AflII CTTAAG 1 cut(s) 1115
AflIII ACRYGT 2 cut(s) 302, 366
AgsI TTSAA 8 cut(s) 136, 271, 755, 890, 920, 1279, 1347, 1369
AleI CACNNNNGTG 1 cut(s) 471
AluBI AGCT 9 cut(s) 599, 746, 758, 793, 821, 962, 996, 1102, 1362
AluI AGCT 9 cut(s) 599, 746, 758, 793, 821, 962, 996, 1102, 1362
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 4 cut(s) 549, 1121, 1135, 1397
AlwNI CAGNNNCTG 1 cut(s) 1420
Ama87I CYCGRG 1 cut(s) 319
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 2 cut(s) 628, 1244
ApeKI GCWGC 3 cut(s) 596, 1093, 1163
ApoI RAATTY 1 cut(s) 651
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
AseI ATTAAT 3 cut(s) 552, 738, 1110
Asp700I GAANNNNTTC 1 cut(s) 1261
Asp718I GGTACC 1 cut(s) 479
AspLEI GCGC 1 cut(s) 1333
AspS9I GGNCC 2 cut(s) 248, 1244
AsuC2I CCSGG 4 cut(s) 275, 320, 321, 1145
AsuHPI GGTGA 2 cut(s) 587, 1375
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 1 cut(s) 248
BaeGI GKGCMC 1 cut(s) 320
BaeI ACNNNNGTAYC 4 cut(s) 397, 430, 699, 732
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 78
BbvCI CCTCAGC 1 cut(s) 963
BbvI GCAGC 3 cut(s) 608, 1105, 1175
BccI CCATC 4 cut(s) 90, 491, 572, 1418
BciVI GTATCC 1 cut(s) 398
BcnI CCSGG 4 cut(s) 275, 320, 321, 1145
BcoDI GTCTC 1 cut(s) 382
BfmI CTRYAG 1 cut(s) 805
BfrI CTTAAG 1 cut(s) 1115
BfuI GTATCC 1 cut(s) 398
BglII AGATCT 2 cut(s) 692, 979
BisI GCNGC 4 cut(s) 597, 623, 1094, 1164
BlsI GCNGC 4 cut(s) 598, 624, 1095, 1165
BmcAI AGTACT 1 cut(s) 387
Bme1390I CCNGG 4 cut(s) 275, 320, 321, 1145
Bme18I GGWCC 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 248, 1244
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 4 cut(s) 275, 320, 321, 1145
BmsI GCATC 2 cut(s) 1009, 1407
BpiI GAAGAC 1 cut(s) 78
Bpu10I CCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 11
BpuMI CCSGG 4 cut(s) 275, 320, 321, 1145
BsaAI YACGTR 1 cut(s) 1134
BsaBI GATNNNNATC 2 cut(s) 12, 1401
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 319, 320, 447
BsaWI WCCGGW 2 cut(s) 679, 868
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 1253, 1420
Bse1I ACTGG 1 cut(s) 840
Bse3DI GCAATG 2 cut(s) 1165, 1221
Bse8I GATNNNNATC 2 cut(s) 12, 1401
BseAI TCCGGA 1 cut(s) 868
BseDI CCNNGG 3 cut(s) 319, 320, 447
BseGI GGATG 6 cut(s) 184, 462, 1024, 1329, 1429, 1464
BseJI GATNNNNATC 2 cut(s) 12, 1401
BseLI CCNNNNNNNGG 2 cut(s) 1253, 1420
BseMI GCAATG 2 cut(s) 1165, 1221
BseMII CTCAG 2 cut(s) 954, 1056
BseNI ACTGG 1 cut(s) 840
BseRI GAGGAG 2 cut(s) 516, 734
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 3 cut(s) 608, 1105, 1175
BsgI GTGCAG 3 cut(s) 50, 990, 1165
Bsh1236I CGCG 2 cut(s) 499, 1333
BshFI GGCC 2 cut(s) 630, 1246
BshNI GGYRCC 1 cut(s) 479
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 7 cut(s) 21, 238, 275, 320, 680, 869, 1144
BslFI GGGAC 4 cut(s) 62, 241, 813, 859
BslI CCNNNNNNNGG 2 cut(s) 1253, 1420
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 4 cut(s) 62, 241, 813, 859
BsmI GAATGC 2 cut(s) 534, 1261
BsnI GGCC 2 cut(s) 630, 1246
BsoBI CYCGRG 1 cut(s) 319
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 320, 327
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
Bsp68I TCGCGA 1 cut(s) 499
BspACI CCGC 4 cut(s) 241, 622, 713, 1202
BspANI GGCC 2 cut(s) 630, 1246
BspCNI CTCAG 2 cut(s) 955, 1057
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 2 cut(s) 499, 1333
BspLI GGNNCC 1 cut(s) 481
BspPI GGATC 4 cut(s) 549, 1121, 1135, 1397
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 1115
BsrDI GCAATG 2 cut(s) 1165, 1221
BsrI ACTGG 1 cut(s) 840
BssECI CCNNGG 3 cut(s) 319, 320, 447
BssMI GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 6 cut(s) 187, 334, 433, 809, 857, 1436
BstACI GRCGYC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 1115
BstBAI YACGTR 1 cut(s) 1134
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 243, 998, 1104
BstDEI CTNAG 2 cut(s) 963, 1065
BstF5I GGATG 6 cut(s) 184, 462, 1024, 1329, 1429, 1464
BstFNI CGCG 2 cut(s) 499, 1333
BstHHI GCGC 1 cut(s) 1333
BstKTI GATC 7 cut(s) 106, 544, 695, 982, 1129, 1143, 1405
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
BstMWI GCNNNNNNNGC 7 cut(s) 57, 731, 827, 836, 968, 1099, 1199
BstNSI RCATGY 4 cut(s) 290, 306, 370, 1477
BstSCI CCNGG 4 cut(s) 273, 318, 319, 1143
BstSFI CTRYAG 1 cut(s) 805
BstSLI GKGCMC 1 cut(s) 320
BstSNI TACGTA 1 cut(s) 1134
BstUI CGCG 2 cut(s) 499, 1333
BstV1I GCAGC 3 cut(s) 608, 1105, 1175
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 4 cut(s) 692, 979, 1126, 1402
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 4 cut(s) 692, 979, 1126, 1402
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 2 cut(s) 630, 1246
BtsCI GGATG 6 cut(s) 184, 462, 1024, 1329, 1429, 1464
BtsIMutI CAGTG 1 cut(s) 814
BtuMI TCGCGA 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 243, 998, 1104
CaiI CAGNNNCTG 1 cut(s) 1420
CfoI GCGC 1 cut(s) 1333
Cfr13I GGNCC 2 cut(s) 248, 1244
Cfr9I CCCGGG 1 cut(s) 319
CseI GACGC 1 cut(s) 810
Csp6I GTAC 7 cut(s) 284, 386, 480, 492, 913, 1135, 1225
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 9 cut(s) 287, 303, 367, 509, 557, 749, 1138, 1208, 1474
CviQI GTAC 7 cut(s) 284, 386, 480, 492, 913, 1135, 1225
DdeI CTNAG 2 cut(s) 963, 1065
DpnI GATC 7 cut(s) 105, 543, 694, 981, 1128, 1142, 1404
DpnII GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
Eco105I TACGTA 1 cut(s) 1134
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 248
Eco57I CTGAAG 2 cut(s) 300, 1008
Eco88I CYCGRG 1 cut(s) 319
EcoRI GAATTC 1 cut(s) 651
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 9 cut(s) 290, 306, 370, 512, 560, 752, 1141, 1211, 1477
FalI AAGNNNNNCTT 9 cut(s) 38, 70, 888, 920, 1067, 1099, 1249, 1281, 1462
FaqI GGGAC 4 cut(s) 62, 241, 813, 859
FatI CATG 9 cut(s) 286, 302, 366, 508, 556, 748, 1137, 1207, 1473
FauI CCCGC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 945
FblI GTMKAC 2 cut(s) 852, 1176
Fnu4HI GCNGC 4 cut(s) 597, 623, 1094, 1164
FokI GGATG 6 cut(s) 191, 449, 1031, 1336, 1436, 1451
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 4 cut(s) 597, 623, 1094, 1164
GlaI GCGC 1 cut(s) 1332
GluI GCNGC 4 cut(s) 597, 623, 1094, 1164
HaeIII GGCC 2 cut(s) 630, 1246
HapII CCGG 7 cut(s) 21, 238, 275, 320, 680, 869, 1144
HgaI GACGC 1 cut(s) 810
HhaI GCGC 1 cut(s) 1333
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 9 cut(s) 290, 306, 370, 512, 560, 752, 1141, 1211, 1477
Hin6I GCGC 1 cut(s) 1331
HinP1I GCGC 1 cut(s) 1331
HincII GTYRAC 3 cut(s) 172, 529, 853
HindII GTYRAC 3 cut(s) 172, 529, 853
HinfI GANTC 8 cut(s) 8, 13, 132, 865, 872, 927, 1078, 1335
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 7 cut(s) 21, 238, 275, 320, 680, 869, 1144
HphI GGTGA 2 cut(s) 587, 1375
Hpy166II GTNNAC 9 cut(s) 172, 208, 356, 529, 562, 853, 902, 1177, 1227
Hpy188I TCNGA 5 cut(s) 91, 103, 222, 991, 1066
Hpy8I GTNNAC 9 cut(s) 172, 208, 356, 529, 562, 853, 902, 1177, 1227
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 6 cut(s) 31, 253, 884, 889, 1083, 1251
HpyCH4III ACNGT 6 cut(s) 187, 334, 433, 809, 857, 1436
HpyCH4IV ACGT 8 cut(s) 27, 63, 75, 174, 494, 730, 1133, 1173
HpyCH4V TGCA 7 cut(s) 31, 518, 647, 971, 1000, 1163, 1182
HpyF10VI GCNNNNNNNGC 7 cut(s) 57, 731, 827, 836, 968, 1099, 1199
HpyF3I CTNAG 2 cut(s) 963, 1065
HpySE526I ACGT 8 cut(s) 27, 63, 75, 174, 494, 730, 1133, 1173
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 9 cut(s) 290, 306, 370, 512, 560, 752, 1141, 1211, 1477
HspAI GCGC 1 cut(s) 1331
Kpn2I TCCGGA 1 cut(s) 868
KpnI GGTACC 1 cut(s) 483
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
LmnI GCTCC 2 cut(s) 593, 1211
Lsp1109I GCAGC 3 cut(s) 608, 1105, 1175
LweI GCATC 2 cut(s) 1009, 1407
MaeII ACGT 8 cut(s) 27, 63, 75, 174, 494, 730, 1133, 1173
MaeIII GTNAC 4 cut(s) 298, 433, 1079, 1381
MalI GATC 7 cut(s) 105, 543, 694, 981, 1128, 1142, 1404
MboI GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
MboII GAAGA 5 cut(s) 83, 98, 974, 1335, 1412
MflI RGATCY 4 cut(s) 692, 979, 1126, 1402
MhlI GDGCHC 2 cut(s) 320, 327
MluCI AATT 6 cut(s) 345, 549, 567, 615, 651, 739
MlyI GAGTC 4 cut(s) 881, 921, 1087, 1329
MmeI TCCRAC 1 cut(s) 1433
MroI TCCGGA 1 cut(s) 868
MroXI GAANNNNTTC 1 cut(s) 1261
MseI TTAA 6 cut(s) 171, 552, 738, 1041, 1110, 1116
MslI CAYNNNNRTG 3 cut(s) 369, 471, 821
MspA1I CMGCKG 1 cut(s) 599
MspCI CTTAAG 1 cut(s) 1115
MspI CCGG 7 cut(s) 21, 238, 275, 320, 680, 869, 1144
MspR9I CCNGG 4 cut(s) 275, 320, 321, 1145
Mva1269I GAATGC 2 cut(s) 534, 1261
MvnI CGCG 2 cut(s) 499, 1333
MwoI GCNNNNNNNGC 7 cut(s) 57, 731, 827, 836, 968, 1099, 1199
NciI CCSGG 4 cut(s) 275, 320, 321, 1145
NdeI CATATG 1 cut(s) 945
NdeII GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
NlaIII CATG 9 cut(s) 290, 306, 370, 512, 560, 752, 1141, 1211, 1477
NlaIV GGNNCC 1 cut(s) 481
NmeAIII GCCGAG 1 cut(s) 1383
NmuCI GTSAC 2 cut(s) 1079, 1381
NruI TCGCGA 1 cut(s) 499
NspI RCATGY 4 cut(s) 290, 306, 370, 1477
NspV TTCGAA 1 cut(s) 11
OliI CACNNNNGTG 1 cut(s) 471
PciI ACATGT 2 cut(s) 302, 366
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PctI GAATGC 2 cut(s) 534, 1261
PdmI GAANNNNTTC 1 cut(s) 1261
PfeI GAWTC 4 cut(s) 8, 13, 132, 865
PflFI GACNNNGTC 1 cut(s) 1172
PflMI CCANNNNNTGG 1 cut(s) 1420
PkrI GCNGC 4 cut(s) 598, 624, 1095, 1165
PleI GAGTC 4 cut(s) 880, 921, 1086, 1329
PpsI GAGTC 4 cut(s) 880, 921, 1086, 1329
Ppu21I YACGTR 1 cut(s) 1134
PscI ACATGT 2 cut(s) 302, 366
PshBI ATTAAT 3 cut(s) 552, 738, 1110
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 2 cut(s) 248, 1244
PstNI CAGNNNCTG 1 cut(s) 1420
PsuI RGATCY 4 cut(s) 692, 979, 1126, 1402
PsyI GACNNNGTC 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 599
RruI TCGCGA 1 cut(s) 499
RsaI GTAC 7 cut(s) 285, 387, 481, 493, 914, 1136, 1226
RsaNI GTAC 7 cut(s) 284, 386, 480, 492, 913, 1135, 1225
RseI CAYNNNNRTG 3 cut(s) 369, 471, 821
SalI GTCGAC 1 cut(s) 851
SaqAI TTAA 6 cut(s) 171, 552, 738, 1041, 1110, 1116
SatI GCNGC 4 cut(s) 597, 623, 1094, 1164
Sau3AI GATC 7 cut(s) 103, 541, 692, 979, 1126, 1140, 1402
Sau96I GGNCC 2 cut(s) 248, 1244
ScaI AGTACT 1 cut(s) 387
SchI GAGTC 4 cut(s) 881, 921, 1087, 1329
ScrFI CCNGG 4 cut(s) 275, 320, 321, 1145
SduI GDGCHC 2 cut(s) 320, 327
SfaNI GCATC 2 cut(s) 1009, 1407
SfcI CTRYAG 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 1 cut(s) 248
SmaI CCCGGG 1 cut(s) 321
SmiMI CAYNNNNRTG 3 cut(s) 369, 471, 821
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SnaBI TACGTA 1 cut(s) 1134
Sse9I AATT 6 cut(s) 345, 549, 567, 615, 651, 739
SsiI CCGC 4 cut(s) 241, 622, 713, 1202
StyD4I CCNGG 4 cut(s) 273, 318, 319, 1143
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 6 cut(s) 187, 334, 433, 809, 857, 1436
TaiI ACGT 8 cut(s) 30, 66, 78, 177, 497, 733, 1136, 1176
TaqI TCGA 5 cut(s) 11, 69, 380, 844, 852
TasI AATT 6 cut(s) 345, 549, 567, 615, 651, 739
TatI WGTACW 3 cut(s) 283, 385, 912
TauI GCSGC 1 cut(s) 625
TfiI GAWTC 4 cut(s) 8, 13, 132, 865
Tru1I TTAA 6 cut(s) 171, 552, 738, 1041, 1110, 1116
Tru9I TTAA 6 cut(s) 171, 552, 738, 1041, 1110, 1116
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 2 cut(s) 1079, 1381
TseI GCWGC 3 cut(s) 596, 1093, 1163
Tsp45I GTSAC 2 cut(s) 1079, 1381
TspDTI ATGAA 5 cut(s) 50, 805, 1022, 1196, 1413
TspGWI ACGGA 3 cut(s) 114, 227, 414
TspMI CCCGGG 1 cut(s) 319
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 1 cut(s) 1172
Van91I CCANNNNNTGG 1 cut(s) 1420
Vha464I CTTAAG 1 cut(s) 1115
VpaK11BI GGWCC 1 cut(s) 248
VspI ATTAAT 3 cut(s) 552, 738, 1110
XapI RAATTY 1 cut(s) 651
XceI RCATGY 4 cut(s) 290, 306, 370, 1477
XcmI CCANNNNNNNNNTGG 2 cut(s) 473, 1421
XmaI CCCGGG 1 cut(s) 319
XmiI GTMKAC 2 cut(s) 852, 1176
XmnI GAANNNNTTC 1 cut(s) 1261
ZraI GACGTC 1 cut(s) 76
ZrmI AGTACT 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.