Rorug06G0352500

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000762
Physical Location & Seq
Forward (+)
50162388 .. 50163809
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0352500.1

Sequence Viewer

Length: 828 bp
ATGTCACGTTCCCACAAAGCCCTCGACTCACGGCACCCCTTAGATTCGTGTACATTTCAACTCCATAGCTGGAGACCTTTCCAGCTTCAACAGCAGACCACCACCGCACCCACCTCCAAAACCCTAGACTCCGATCCCACCAACCCCAAACCCTACTACCCCAAGCGTCCATGCCTCTCCAACCGAGCGACGTCGTTTTCCATCGACGCCATCGACATGTCCAGGCTGACTTTGGTCGACGACGACCGGACGATCTCCGGAGGGCACCATAAGCACGGGAGCTTCCGATTCATTGACAAGAAGAGGCGGCGGCGCGGGTCCAGGTCGGTTTCGGGTCGGAGCAGCGATCGGAGCGGGACCCGGAGGTGCTGCTCCGTCGGGGCTTCGGCTGCTTACGGGACGTGTTCGGATTTTCCGGTGGCGATTGGTACGGACTCGAGTGGGGAGCTGTTTGGGAATGGGGATGCGAATTGGGCCTCGGATGTGAGTGAGGCTAGGAATTCGAGGAAGGAGAGGGATGGAGTTGGGAGTGGAGAGAAGGAGAATCCGGGTGTCGGGTTTGGGCCGAGTGGGGGCTTTGATGCTCAGGGAAATGAGTCCGGATATGGTAGTGAACCGGGTTACCGTGGGGACGCGGAGTTCGGATATGGTGATGAACTTGATGAGGAGGAGGATGATGCTCGTCTATTGTTTTGGGGCAATCATTTCGGAGATAGTGATACTATGATGGAGGTTGTGGGTGAGAATACTTTCACGGATCAAAAATCGCATCACAGATGTCGCCGTAAGAAACATGATTGCAGAATGGTTGTTGATTCCCTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

275

Amino Acids

30.39

Weight (kDa)

6.56

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 33, 264
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 315, 635
AccIII TCCGGA 2 cut(s) 257, 599
AciI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
AclWI GGATC 2 cut(s) 128, 765
AcsI RAATTY 1 cut(s) 499
AcyI GRCGYC 2 cut(s) 191, 207
AfaI GTAC 2 cut(s) 52, 430
AfiI CCNNNNNNNGG 2 cut(s) 554, 572
AflIII ACRYGT 2 cut(s) 216, 401
AgsI TTSAA 2 cut(s) 59, 89
AjiI CACGTC 1 cut(s) 402
AjnI CCWGG 2 cut(s) 221, 320
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 4 cut(s) 69, 85, 282, 448
AluI AGCT 4 cut(s) 69, 85, 282, 448
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 128, 765
Ama87I CYCGRG 1 cut(s) 436
Aor13HI TCCGGA 2 cut(s) 257, 599
AoxI GGCC 2 cut(s) 474, 563
ApeKI GCWGC 3 cut(s) 342, 369, 389
ApoI RAATTY 1 cut(s) 499
ArsI GACNNNNNNTTYG 2 cut(s) 623, 655
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 4 cut(s) 318, 357, 474, 563
AsuC2I CCSGG 3 cut(s) 361, 549, 618
AsuHPI GGTGA 2 cut(s) 662, 752
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 318, 357
AxyI CCTNAGG 1 cut(s) 821
BaeGI GKGCMC 1 cut(s) 267
BanI GGYRCC 2 cut(s) 33, 264
BbvI GCAGC 3 cut(s) 354, 356, 376
BccI CCATC 4 cut(s) 209, 218, 512, 721
BceAI ACGGC 2 cut(s) 47, 768
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 2 cut(s) 223, 322
BcnI CCSGG 3 cut(s) 361, 549, 618
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 125, 495
BisI GCNGC 5 cut(s) 308, 311, 343, 370, 390
BlsI GCNGC 5 cut(s) 309, 312, 344, 371, 391
Bme1390I CCNGG 5 cut(s) 223, 322, 361, 549, 618
Bme18I GGWCC 2 cut(s) 318, 357
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 402
BmgT120I GGNCC 4 cut(s) 318, 357, 474, 563
BmiI GGNNCC 5 cut(s) 35, 266, 319, 358, 359
BmrFI CCNGG 5 cut(s) 223, 322, 361, 549, 618
BmsI GCATC 4 cut(s) 454, 571, 667, 778
BoxI GACNNNNGTC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 585
BpuMI CCSGG 3 cut(s) 361, 549, 618
BsaHI GRCGYC 2 cut(s) 191, 207
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 477, 625
BsaWI WCCGGW 4 cut(s) 246, 257, 415, 599
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 572
Bse21I CCTNAGG 1 cut(s) 821
BseAI TCCGGA 2 cut(s) 257, 599
BseBI CCWGG 2 cut(s) 223, 322
BseDI CCNNGG 2 cut(s) 477, 625
BseGI GGATG 4 cut(s) 469, 487, 523, 679
BseLI CCNNNNNNNGG 2 cut(s) 554, 572
BseMII CTCAG 2 cut(s) 599, 812
BseRI GAGGAG 2 cut(s) 680, 683
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 3 cut(s) 354, 356, 376
Bsh1236I CGCG 2 cut(s) 315, 635
Bsh1285I CGRYCG 2 cut(s) 247, 349
BshFI GGCC 2 cut(s) 476, 565
BshNI GGYRCC 2 cut(s) 33, 264
BsiEI CGRYCG 2 cut(s) 247, 349
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 7 cut(s) 247, 258, 361, 416, 548, 600, 617
BslFI GGGAC 3 cut(s) 370, 412, 644
BslI CCNNNNNNNGG 2 cut(s) 554, 572
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 3 cut(s) 370, 412, 644
BsnI GGCC 2 cut(s) 476, 565
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 267
Bsp13I TCCGGA 2 cut(s) 257, 599
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 4 cut(s) 133, 252, 346, 757
BspACI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
BspANI GGCC 2 cut(s) 476, 565
BspCNI CTCAG 2 cut(s) 598, 813
BspEI TCCGGA 2 cut(s) 257, 599
BspFNI CGCG 2 cut(s) 315, 635
BspLI GGNNCC 5 cut(s) 35, 266, 319, 358, 359
BspPI GGATC 2 cut(s) 128, 765
BspT107I GGYRCC 2 cut(s) 33, 264
BspTNI GGTCTC 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 354
BsrGI TGTACA 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 477, 625
BssMI GATC 4 cut(s) 133, 252, 346, 757
BssNI GRCGYC 2 cut(s) 191, 207
Bst2UI CCWGG 2 cut(s) 223, 322
Bst4CI ACNGT 1 cut(s) 626
Bst6I CTCTTC 1 cut(s) 296
BstACI GRCGYC 2 cut(s) 191, 207
BstAUI TGTACA 1 cut(s) 50
BstDEI CTNAG 3 cut(s) 40, 585, 821
BstDSI CCRYGG 1 cut(s) 625
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 4 cut(s) 469, 487, 523, 679
BstFNI CGCG 2 cut(s) 315, 635
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 4 cut(s) 136, 255, 349, 760
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 4 cut(s) 133, 252, 346, 757
BstMCI CGRYCG 2 cut(s) 247, 349
BstMWI GCNNNNNNNGC 5 cut(s) 91, 271, 351, 389, 473
BstNI CCWGG 2 cut(s) 223, 322
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 233
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 5 cut(s) 221, 320, 359, 547, 616
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 2 cut(s) 315, 635
BstV1I GCAGC 3 cut(s) 354, 356, 376
Bsu36I CCTNAGG 1 cut(s) 821
BsuRI GGCC 2 cut(s) 476, 565
BtgI CCRYGG 1 cut(s) 625
BtrI CACGTC 1 cut(s) 402
BtsCI GGATG 4 cut(s) 469, 487, 523, 679
CfoI GCGC 1 cut(s) 315
Cfr13I GGNCC 4 cut(s) 318, 357, 474, 563
CseI GACGC 3 cut(s) 155, 215, 641
Csp6I GTAC 2 cut(s) 51, 429
CviAII CATG 3 cut(s) 171, 217, 794
CviQI GTAC 2 cut(s) 51, 429
DdeI CTNAG 3 cut(s) 40, 585, 821
DpnI GATC 4 cut(s) 135, 254, 348, 759
DpnII GATC 4 cut(s) 133, 252, 346, 757
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 318, 357
Eco81I CCTNAGG 1 cut(s) 821
Eco88I CYCGRG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 357
EcoO65I GGTNACC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 2 cut(s) 221, 320
FaeI CATG 3 cut(s) 174, 220, 797
FaiI YATR 8 cut(s) 66, 172, 218, 270, 606, 648, 725, 795
FaqI GGGAC 3 cut(s) 370, 412, 644
FatI CATG 3 cut(s) 170, 216, 793
FauI CCCGC 2 cut(s) 308, 347
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 5 cut(s) 308, 311, 343, 370, 390
FokI GGATG 4 cut(s) 476, 494, 530, 686
Fsp4HI GCNGC 5 cut(s) 308, 311, 343, 370, 390
FspBI CTAG 2 cut(s) 125, 495
GlaI GCGC 1 cut(s) 314
GluI GCNGC 5 cut(s) 308, 311, 343, 370, 390
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 2 cut(s) 476, 565
HapII CCGG 7 cut(s) 247, 258, 361, 416, 548, 600, 617
HgaI GACGC 3 cut(s) 155, 215, 641
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 2 cut(s) 191, 207
Hin1II CATG 3 cut(s) 174, 220, 797
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 8 cut(s) 26, 44, 128, 288, 434, 544, 596, 815
HpaII CCGG 7 cut(s) 247, 258, 361, 416, 548, 600, 617
HphI GGTGA 2 cut(s) 662, 752
Hpy166II GTNNAC 3 cut(s) 51, 238, 614
Hpy188I TCNGA 8 cut(s) 133, 287, 339, 351, 409, 481, 644, 710
Hpy188III TCNNGA 2 cut(s) 258, 600
Hpy8I GTNNAC 3 cut(s) 51, 238, 614
Hpy99I CGWCG 6 cut(s) 193, 196, 209, 242, 245, 380
HpyAV CCTTC 2 cut(s) 502, 532
HpyCH4III ACNGT 1 cut(s) 626
HpyCH4IV ACGT 3 cut(s) 7, 191, 401
HpyCH4V TGCA 1 cut(s) 801
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 271, 351, 389, 473
HpyF3I CTNAG 3 cut(s) 40, 585, 821
HpySE526I ACGT 3 cut(s) 7, 191, 401
Hsp92I GRCGYC 2 cut(s) 191, 207
Hsp92II CATG 3 cut(s) 174, 220, 797
HspAI GCGC 1 cut(s) 313
KflI GGGWCCC 1 cut(s) 357
Kpn2I TCCGGA 2 cut(s) 257, 599
Kzo9I GATC 4 cut(s) 133, 252, 346, 757
LmnI GCTCC 5 cut(s) 279, 339, 351, 377, 445
Lsp1109I GCAGC 3 cut(s) 354, 356, 376
LweI GCATC 4 cut(s) 454, 571, 667, 778
MaeI CTAG 2 cut(s) 125, 495
MaeII ACGT 3 cut(s) 7, 191, 401
MaeIII GTNAC 2 cut(s) 3, 620
MalI GATC 4 cut(s) 135, 254, 348, 759
MbiI CCGCTC 1 cut(s) 354
MboI GATC 4 cut(s) 133, 252, 346, 757
MboII GAAGA 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 2 cut(s) 469, 499
MlyI GAGTC 4 cut(s) 20, 122, 428, 605
MmeI TCCRAC 2 cut(s) 204, 317
MroI TCCGGA 2 cut(s) 257, 599
MroXI GAANNNNTTC 1 cut(s) 749
MslI CAYNNNNRTG 1 cut(s) 215
MspI CCGG 7 cut(s) 247, 258, 361, 416, 548, 600, 617
MspR9I CCNGG 5 cut(s) 223, 322, 361, 549, 618
MvaI CCWGG 2 cut(s) 223, 322
MvnI CGCG 2 cut(s) 315, 635
MwoI GCNNNNNNNGC 5 cut(s) 91, 271, 351, 389, 473
NciI CCSGG 3 cut(s) 361, 549, 618
NdeII GATC 4 cut(s) 133, 252, 346, 757
NlaIII CATG 3 cut(s) 174, 220, 797
NlaIV GGNNCC 5 cut(s) 35, 266, 319, 358, 359
NmeAIII GCCGAG 1 cut(s) 591
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 436
PciI ACATGT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 4 cut(s) 44, 288, 544, 815
PkrI GCNGC 5 cut(s) 309, 312, 344, 371, 391
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 4 cut(s) 20, 122, 428, 604
PpsI GAGTC 4 cut(s) 20, 122, 428, 604
PpuMI RGGWCCY 1 cut(s) 357
PscI ACATGT 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 233
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 221, 320
PspEI GGTNACC 1 cut(s) 620
PspGI CCWGG 2 cut(s) 221, 320
PspN4I GGNNCC 5 cut(s) 35, 266, 319, 358, 359
PspPI GGNCC 4 cut(s) 318, 357, 474, 563
PspPPI RGGWCCY 1 cut(s) 357
PspXI VCTCGAGB 1 cut(s) 436
PvuI CGATCG 1 cut(s) 349
RsaI GTAC 2 cut(s) 52, 430
RsaNI GTAC 2 cut(s) 51, 429
RseI CAYNNNNRTG 1 cut(s) 215
SalI GTCGAC 1 cut(s) 236
SatI GCNGC 5 cut(s) 308, 311, 343, 370, 390
Sau3AI GATC 4 cut(s) 133, 252, 346, 757
Sau96I GGNCC 4 cut(s) 318, 357, 474, 563
SchI GAGTC 4 cut(s) 20, 122, 428, 605
ScrFI CCNGG 5 cut(s) 223, 322, 361, 549, 618
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 4 cut(s) 454, 571, 667, 778
Sfr274I CTCGAG 1 cut(s) 436
SinI GGWCC 2 cut(s) 318, 357
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 2 cut(s) 469, 499
SsiI CCGC 6 cut(s) 105, 307, 310, 315, 354, 635
SspMI CTAG 2 cut(s) 125, 495
StyD4I CCNGG 5 cut(s) 221, 320, 359, 547, 616
TaaI ACNGT 1 cut(s) 626
TaiI ACGT 3 cut(s) 10, 194, 404
TaqI TCGA 6 cut(s) 24, 204, 213, 237, 437, 503
TasI AATT 2 cut(s) 469, 499
TatI WGTACW 1 cut(s) 50
TauI GCSGC 2 cut(s) 310, 313
TfiI GAWTC 4 cut(s) 44, 288, 544, 815
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 342, 369, 389
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 280, 669
TspGWI ACGGA 3 cut(s) 364, 446, 770
VpaK11BI GGWCC 2 cut(s) 318, 357
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 125, 495
ZraI GACGTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.