Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 78 |
| Acc65I | GGTACC | 1 cut(s) | 479 |
| AccB1I | GGYRCC | 1 cut(s) | 479 |
| AccI | GTMKAC | 2 cut(s) | 852, 1176 |
| AccII | CGCG | 1 cut(s) | 1333 |
| AciI | CCGC | 4 cut(s) | 17, 477, 622, 1202 |
| AclWI | GGATC | 4 cut(s) | 549, 1121, 1135, 1400 |
| AcsI | RAATTY | 1 cut(s) | 651 |
| AcuI | CTGAAG | 2 cut(s) | 300, 1008 |
| AcyI | GRCGYC | 1 cut(s) | 75 |
| AfaI | GTAC | 6 cut(s) | 387, 481, 493, 914, 1136, 1226 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| AflII | CTTAAG | 1 cut(s) | 1115 |
| AflIII | ACRYGT | 2 cut(s) | 302, 366 |
| AgsI | TTSAA | 9 cut(s) | 136, 271, 755, 890, 920, 1043, 1279, 1347, 1369 |
| AleI | CACNNNNGTG | 1 cut(s) | 471 |
| Alw21I | GWGCWC | 1 cut(s) | 69 |
| Alw26I | GTCTC | 1 cut(s) | 382 |
| AlwI | GGATC | 4 cut(s) | 549, 1121, 1135, 1400 |
| Ama87I | CYCGRG | 1 cut(s) | 319 |
| AoxI | GGCC | 2 cut(s) | 628, 1244 |
| ApeKI | GCWGC | 4 cut(s) | 596, 743, 1093, 1163 |
| ApoI | RAATTY | 1 cut(s) | 651 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 680, 712 |
| AseI | ATTAAT | 3 cut(s) | 552, 738, 1110 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1261 |
| Asp718I | GGTACC | 1 cut(s) | 479 |
| AspLEI | GCGC | 1 cut(s) | 1333 |
| AspS9I | GGNCC | 2 cut(s) | 248, 1244 |
| AsuC2I | CCSGG | 4 cut(s) | 21, 320, 321, 1145 |
| AsuHPI | GGTGA | 3 cut(s) | 346, 587, 1375 |
| AsuII | TTCGAA | 1 cut(s) | 11 |
| AvaI | CYCGRG | 1 cut(s) | 319 |
| AvaII | GGWCC | 1 cut(s) | 248 |
| BaeGI | GKGCMC | 1 cut(s) | 320 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 397, 430, 699, 732 |
| BanI | GGYRCC | 1 cut(s) | 479 |
| BanII | GRGCYC | 1 cut(s) | 327 |
| BauI | CACGAG | 1 cut(s) | 498 |
| BbsI | GAAGAC | 2 cut(s) | 78, 1404 |
| Bbv12I | GWGCWC | 1 cut(s) | 69 |
| BbvCI | CCTCAGC | 1 cut(s) | 963 |
| BbvI | GCAGC | 4 cut(s) | 608, 755, 1105, 1175 |
| BccI | CCATC | 4 cut(s) | 90, 491, 572, 1421 |
| BciVI | GTATCC | 1 cut(s) | 398 |
| BclI | TGATCA | 1 cut(s) | 336 |
| BcnI | CCSGG | 4 cut(s) | 21, 320, 321, 1145 |
| BcoDI | GTCTC | 1 cut(s) | 382 |
| BfmI | CTRYAG | 2 cut(s) | 711, 805 |
| BfrI | CTTAAG | 1 cut(s) | 1115 |
| BfuI | GTATCC | 1 cut(s) | 398 |
| BglII | AGATCT | 2 cut(s) | 692, 979 |
| BisI | GCNGC | 5 cut(s) | 597, 623, 744, 1094, 1164 |
| BlsI | GCNGC | 5 cut(s) | 598, 624, 745, 1095, 1165 |
| BmcAI | AGTACT | 1 cut(s) | 387 |
| Bme1390I | CCNGG | 4 cut(s) | 21, 320, 321, 1145 |
| Bme18I | GGWCC | 1 cut(s) | 248 |
| BmeT110I | CYCGRG | 1 cut(s) | 319 |
| BmgT120I | GGNCC | 2 cut(s) | 248, 1244 |
| BmiI | GGNNCC | 1 cut(s) | 481 |
| BmrFI | CCNGG | 4 cut(s) | 21, 320, 321, 1145 |
| BmsI | GCATC | 1 cut(s) | 1009 |
| BpiI | GAAGAC | 2 cut(s) | 78, 1404 |
| Bpu10I | CCTNAGC | 1 cut(s) | 963 |
| Bpu14I | TTCGAA | 1 cut(s) | 11 |
| BpuEI | CTTGAG | 1 cut(s) | 914 |
| BpuMI | CCSGG | 4 cut(s) | 21, 320, 321, 1145 |
| BsaAI | YACGTR | 2 cut(s) | 64, 1134 |
| BsaBI | GATNNNNATC | 1 cut(s) | 12 |
| BsaHI | GRCGYC | 1 cut(s) | 75 |
| BsaJI | CCNNGG | 3 cut(s) | 319, 320, 447 |
| BsaWI | WCCGGW | 1 cut(s) | 679 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 585, 615 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1253 |
| Bse1I | ACTGG | 1 cut(s) | 840 |
| Bse3DI | GCAATG | 2 cut(s) | 1165, 1221 |
| Bse8I | GATNNNNATC | 1 cut(s) | 12 |
| BseDI | CCNNGG | 3 cut(s) | 319, 320, 447 |
| BseGI | GGATG | 5 cut(s) | 184, 1024, 1329, 1432, 1467 |
| BseJI | GATNNNNATC | 1 cut(s) | 12 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| BseMI | GCAATG | 2 cut(s) | 1165, 1221 |
| BseMII | CTCAG | 3 cut(s) | 954, 1056, 1430 |
| BseNI | ACTGG | 1 cut(s) | 840 |
| BseRI | GAGGAG | 2 cut(s) | 516, 734 |
| BseSI | GKGCMC | 1 cut(s) | 320 |
| BseXI | GCAGC | 4 cut(s) | 608, 755, 1105, 1175 |
| BseYI | CCCAGC | 2 cut(s) | 274, 1472 |
| BsgI | GTGCAG | 3 cut(s) | 50, 990, 1165 |
| Bsh1236I | CGCG | 1 cut(s) | 1333 |
| BshFI | GGCC | 2 cut(s) | 630, 1246 |
| BshNI | GGYRCC | 1 cut(s) | 479 |
| BsiHKAI | GWGCWC | 1 cut(s) | 69 |
| BsiHKCI | CYCGRG | 1 cut(s) | 319 |
| BsiSI | CCGG | 5 cut(s) | 21, 238, 320, 680, 1144 |
| BslFI | GGGAC | 4 cut(s) | 62, 241, 813, 859 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1253 |
| BsmAI | GTCTC | 1 cut(s) | 382 |
| BsmFI | GGGAC | 4 cut(s) | 62, 241, 813, 859 |
| BsmI | GAATGC | 2 cut(s) | 534, 1261 |
| BsnI | GGCC | 2 cut(s) | 630, 1246 |
| BsoBI | CYCGRG | 1 cut(s) | 319 |
| Bsp119I | TTCGAA | 1 cut(s) | 11 |
| Bsp1286I | GDGCHC | 3 cut(s) | 69, 320, 327 |
| Bsp143I | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| BspACI | CCGC | 4 cut(s) | 17, 477, 622, 1202 |
| BspANI | GGCC | 2 cut(s) | 630, 1246 |
| BspCNI | CTCAG | 3 cut(s) | 955, 1057, 1429 |
| BspFNI | CGCG | 1 cut(s) | 1333 |
| BspHI | TCATGA | 1 cut(s) | 1481 |
| BspLI | GGNNCC | 1 cut(s) | 481 |
| BspMAI | CTGCAG | 1 cut(s) | 715 |
| BspPI | GGATC | 4 cut(s) | 549, 1121, 1135, 1400 |
| BspT104I | TTCGAA | 1 cut(s) | 11 |
| BspT107I | GGYRCC | 1 cut(s) | 479 |
| BspTI | CTTAAG | 1 cut(s) | 1115 |
| BsrDI | GCAATG | 2 cut(s) | 1165, 1221 |
| BsrI | ACTGG | 1 cut(s) | 840 |
| BssECI | CCNNGG | 3 cut(s) | 319, 320, 447 |
| BssMI | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| BssNI | GRCGYC | 1 cut(s) | 75 |
| BssSI | CACGAG | 1 cut(s) | 498 |
| BssT1I | CCWWGG | 1 cut(s) | 447 |
| Bst2BI | CACGAG | 1 cut(s) | 498 |
| Bst4CI | ACNGT | 6 cut(s) | 187, 334, 776, 809, 857, 1439 |
| BstACI | GRCGYC | 1 cut(s) | 75 |
| BstAFI | CTTAAG | 1 cut(s) | 1115 |
| BstBAI | YACGTR | 2 cut(s) | 64, 1134 |
| BstBI | TTCGAA | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 3 cut(s) | 243, 998, 1104 |
| BstDEI | CTNAG | 3 cut(s) | 963, 1065, 1416 |
| BstF5I | GGATG | 5 cut(s) | 184, 1024, 1329, 1432, 1467 |
| BstFNI | CGCG | 1 cut(s) | 1333 |
| BstHHI | GCGC | 1 cut(s) | 1333 |
| BstKTI | GATC | 8 cut(s) | 106, 339, 544, 695, 982, 1129, 1143, 1408 |
| BstMAI | GTCTC | 1 cut(s) | 382 |
| BstMBI | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 57, 731, 740, 827, 836, 968, 1099, 1199 |
| BstNSI | RCATGY | 3 cut(s) | 306, 370, 1480 |
| BstSCI | CCNGG | 4 cut(s) | 19, 318, 319, 1143 |
| BstSFI | CTRYAG | 2 cut(s) | 711, 805 |
| BstSLI | GKGCMC | 1 cut(s) | 320 |
| BstSNI | TACGTA | 1 cut(s) | 1134 |
| BstUI | CGCG | 1 cut(s) | 1333 |
| BstV1I | GCAGC | 4 cut(s) | 608, 755, 1105, 1175 |
| BstV2I | GAAGAC | 2 cut(s) | 78, 1404 |
| BstX2I | RGATCY | 4 cut(s) | 692, 979, 1126, 1405 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 473 |
| BstYI | RGATCY | 4 cut(s) | 692, 979, 1126, 1405 |
| BsuI | GTATCC | 1 cut(s) | 398 |
| BsuRI | GGCC | 2 cut(s) | 630, 1246 |
| BtsCI | GGATG | 5 cut(s) | 184, 1024, 1329, 1432, 1467 |
| BtsIMutI | CAGTG | 1 cut(s) | 814 |
| Cac8I | GCNNGC | 3 cut(s) | 243, 998, 1104 |
| CciI | TCATGA | 1 cut(s) | 1481 |
| CfoI | GCGC | 1 cut(s) | 1333 |
| Cfr13I | GGNCC | 2 cut(s) | 248, 1244 |
| Cfr9I | CCCGGG | 1 cut(s) | 319 |
| CseI | GACGC | 1 cut(s) | 810 |
| Csp6I | GTAC | 6 cut(s) | 386, 480, 492, 913, 1135, 1225 |
| CviQI | GTAC | 6 cut(s) | 386, 480, 492, 913, 1135, 1225 |
| DdeI | CTNAG | 3 cut(s) | 963, 1065, 1416 |
| DpnI | GATC | 8 cut(s) | 105, 338, 543, 694, 981, 1128, 1142, 1407 |
| DpnII | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| EciI | GGCGGA | 1 cut(s) | 6 |
| Eco105I | TACGTA | 1 cut(s) | 1134 |
| Eco130I | CCWWGG | 1 cut(s) | 447 |
| Eco24I | GRGCYC | 1 cut(s) | 327 |
| Eco32I | GATATC | 1 cut(s) | 463 |
| Eco47I | GGWCC | 1 cut(s) | 248 |
| Eco57I | CTGAAG | 2 cut(s) | 300, 1008 |
| Eco88I | CYCGRG | 1 cut(s) | 319 |
| EcoRI | GAATTC | 1 cut(s) | 651 |
| EcoRV | GATATC | 1 cut(s) | 463 |
| EcoT14I | CCWWGG | 1 cut(s) | 447 |
| EcoT38I | GRGCYC | 1 cut(s) | 327 |
| ErhI | CCWWGG | 1 cut(s) | 447 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 38, 70, 888, 920, 1067, 1099, 1249, 1281 |
| FaqI | GGGAC | 4 cut(s) | 62, 241, 813, 859 |
| FauNDI | CATATG | 1 cut(s) | 945 |
| FbaI | TGATCA | 1 cut(s) | 336 |
| FblI | GTMKAC | 2 cut(s) | 852, 1176 |
| Fnu4HI | GCNGC | 5 cut(s) | 597, 623, 744, 1094, 1164 |
| FokI | GGATG | 5 cut(s) | 191, 1031, 1336, 1439, 1454 |
| FriOI | GRGCYC | 1 cut(s) | 327 |
| Fsp4HI | GCNGC | 5 cut(s) | 597, 623, 744, 1094, 1164 |
| GlaI | GCGC | 1 cut(s) | 1332 |
| GluI | GCNGC | 5 cut(s) | 597, 623, 744, 1094, 1164 |
| GsaI | CCCAGC | 2 cut(s) | 278, 1476 |
| HaeIII | GGCC | 2 cut(s) | 630, 1246 |
| HapII | CCGG | 5 cut(s) | 21, 238, 320, 680, 1144 |
| HgaI | GACGC | 1 cut(s) | 810 |
| HhaI | GCGC | 1 cut(s) | 1333 |
| Hin1I | GRCGYC | 1 cut(s) | 75 |
| Hin6I | GCGC | 1 cut(s) | 1331 |
| HinP1I | GCGC | 1 cut(s) | 1331 |
| HincII | GTYRAC | 3 cut(s) | 172, 529, 853 |
| HindII | GTYRAC | 3 cut(s) | 172, 529, 853 |
| HinfI | GANTC | 8 cut(s) | 8, 13, 132, 865, 872, 927, 1078, 1335 |
| HpaI | GTTAAC | 1 cut(s) | 172 |
| HpaII | CCGG | 5 cut(s) | 21, 238, 320, 680, 1144 |
| HphI | GGTGA | 3 cut(s) | 346, 587, 1375 |
| Hpy166II | GTNNAC | 9 cut(s) | 172, 208, 356, 529, 562, 853, 902, 1177, 1227 |
| Hpy188I | TCNGA | 5 cut(s) | 103, 222, 991, 1066, 1419 |
| Hpy8I | GTNNAC | 9 cut(s) | 172, 208, 356, 529, 562, 853, 902, 1177, 1227 |
| HpyAV | CCTTC | 6 cut(s) | 31, 253, 884, 889, 1083, 1251 |
| HpyCH4III | ACNGT | 6 cut(s) | 187, 334, 776, 809, 857, 1439 |
| HpyCH4IV | ACGT | 8 cut(s) | 27, 63, 75, 174, 494, 730, 1133, 1173 |
| HpyCH4V | TGCA | 9 cut(s) | 31, 518, 647, 713, 743, 971, 1000, 1163, 1182 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 57, 731, 740, 827, 836, 968, 1099, 1199 |
| HpyF3I | CTNAG | 3 cut(s) | 963, 1065, 1416 |
| HpySE526I | ACGT | 8 cut(s) | 27, 63, 75, 174, 494, 730, 1133, 1173 |
| Hsp92I | GRCGYC | 1 cut(s) | 75 |
| HspAI | GCGC | 1 cut(s) | 1331 |
| KpnI | GGTACC | 1 cut(s) | 483 |
| Ksp22I | TGATCA | 1 cut(s) | 336 |
| KspAI | GTTAAC | 1 cut(s) | 172 |
| Kzo9I | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| LmnI | GCTCC | 1 cut(s) | 593 |
| Lsp1109I | GCAGC | 4 cut(s) | 608, 755, 1105, 1175 |
| LweI | GCATC | 1 cut(s) | 1009 |
| MaeII | ACGT | 8 cut(s) | 27, 63, 75, 174, 494, 730, 1133, 1173 |
| MaeIII | GTNAC | 4 cut(s) | 298, 495, 1079, 1381 |
| MalI | GATC | 8 cut(s) | 105, 338, 543, 694, 981, 1128, 1142, 1407 |
| MboI | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| MboII | GAAGA | 6 cut(s) | 83, 98, 974, 1335, 1409, 1415 |
| MflI | RGATCY | 4 cut(s) | 692, 979, 1126, 1405 |
| MhlI | GDGCHC | 3 cut(s) | 69, 320, 327 |
| MluCI | AATT | 5 cut(s) | 345, 549, 567, 615, 651 |
| MlyI | GAGTC | 4 cut(s) | 881, 921, 1087, 1329 |
| MmeI | TCCRAC | 2 cut(s) | 1404, 1436 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1261 |
| MseI | TTAA | 5 cut(s) | 171, 552, 738, 1110, 1116 |
| MslI | CAYNNNNRTG | 3 cut(s) | 369, 471, 821 |
| MspA1I | CMGCKG | 2 cut(s) | 278, 599 |
| MspCI | CTTAAG | 1 cut(s) | 1115 |
| MspI | CCGG | 5 cut(s) | 21, 238, 320, 680, 1144 |
| MspR9I | CCNGG | 4 cut(s) | 21, 320, 321, 1145 |
| Mva1269I | GAATGC | 2 cut(s) | 534, 1261 |
| MvnI | CGCG | 1 cut(s) | 1333 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 57, 731, 740, 827, 836, 968, 1099, 1199 |
| NciI | CCSGG | 4 cut(s) | 21, 320, 321, 1145 |
| NdeI | CATATG | 1 cut(s) | 945 |
| NdeII | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| NlaIV | GGNNCC | 1 cut(s) | 481 |
| NmeAIII | GCCGAG | 1 cut(s) | 1383 |
| NmuCI | GTSAC | 3 cut(s) | 495, 1079, 1381 |
| NspI | RCATGY | 3 cut(s) | 306, 370, 1480 |
| NspV | TTCGAA | 1 cut(s) | 11 |
| OliI | CACNNNNGTG | 1 cut(s) | 471 |
| PagI | TCATGA | 1 cut(s) | 1481 |
| PciI | ACATGT | 2 cut(s) | 302, 366 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 218 |
| PctI | GAATGC | 2 cut(s) | 534, 1261 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1261 |
| PfeI | GAWTC | 4 cut(s) | 8, 13, 132, 865 |
| PflFI | GACNNNGTC | 1 cut(s) | 1172 |
| PkrI | GCNGC | 5 cut(s) | 598, 624, 745, 1095, 1165 |
| PleI | GAGTC | 4 cut(s) | 880, 921, 1086, 1329 |
| PpsI | GAGTC | 4 cut(s) | 880, 921, 1086, 1329 |
| Ppu21I | YACGTR | 2 cut(s) | 64, 1134 |
| PscI | ACATGT | 2 cut(s) | 302, 366 |
| PshBI | ATTAAT | 3 cut(s) | 552, 738, 1110 |
| PspFI | CCCAGC | 2 cut(s) | 274, 1472 |
| PspN4I | GGNNCC | 1 cut(s) | 481 |
| PspPI | GGNCC | 2 cut(s) | 248, 1244 |
| PstI | CTGCAG | 1 cut(s) | 715 |
| PsuI | RGATCY | 4 cut(s) | 692, 979, 1126, 1405 |
| PsyI | GACNNNGTC | 1 cut(s) | 1172 |
| PvuII | CAGCTG | 2 cut(s) | 278, 599 |
| RsaI | GTAC | 6 cut(s) | 387, 481, 493, 914, 1136, 1226 |
| RsaNI | GTAC | 6 cut(s) | 386, 480, 492, 913, 1135, 1225 |
| RseI | CAYNNNNRTG | 3 cut(s) | 369, 471, 821 |
| SalI | GTCGAC | 1 cut(s) | 851 |
| SaqAI | TTAA | 5 cut(s) | 171, 552, 738, 1110, 1116 |
| SatI | GCNGC | 5 cut(s) | 597, 623, 744, 1094, 1164 |
| Sau3AI | GATC | 8 cut(s) | 103, 336, 541, 692, 979, 1126, 1140, 1405 |
| Sau96I | GGNCC | 2 cut(s) | 248, 1244 |
| ScaI | AGTACT | 1 cut(s) | 387 |
| SchI | GAGTC | 4 cut(s) | 881, 921, 1087, 1329 |
| ScrFI | CCNGG | 4 cut(s) | 21, 320, 321, 1145 |
| SduI | GDGCHC | 3 cut(s) | 69, 320, 327 |
| SfaNI | GCATC | 1 cut(s) | 1009 |
| SfcI | CTRYAG | 2 cut(s) | 711, 805 |
| SfuI | TTCGAA | 1 cut(s) | 11 |
| SinI | GGWCC | 1 cut(s) | 248 |
| SmaI | CCCGGG | 1 cut(s) | 321 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 369, 471, 821 |
| SmlI | CTYRAG | 2 cut(s) | 929, 1115 |
| SmoI | CTYRAG | 2 cut(s) | 929, 1115 |
| SnaBI | TACGTA | 1 cut(s) | 1134 |
| Sse9I | AATT | 5 cut(s) | 345, 549, 567, 615, 651 |
| SsiI | CCGC | 4 cut(s) | 17, 477, 622, 1202 |
| StyD4I | CCNGG | 4 cut(s) | 19, 318, 319, 1143 |
| StyI | CCWWGG | 1 cut(s) | 447 |
| TaaI | ACNGT | 6 cut(s) | 187, 334, 776, 809, 857, 1439 |
| TaiI | ACGT | 8 cut(s) | 30, 66, 78, 177, 497, 733, 1136, 1176 |
| TaqI | TCGA | 5 cut(s) | 11, 69, 380, 844, 852 |
| TasI | AATT | 5 cut(s) | 345, 549, 567, 615, 651 |
| TatI | WGTACW | 2 cut(s) | 385, 912 |
| TauI | GCSGC | 1 cut(s) | 625 |
| TfiI | GAWTC | 4 cut(s) | 8, 13, 132, 865 |
| Tru1I | TTAA | 5 cut(s) | 171, 552, 738, 1110, 1116 |
| Tru9I | TTAA | 5 cut(s) | 171, 552, 738, 1110, 1116 |
| TscAI | CASTG | 1 cut(s) | 814 |
| TseFI | GTSAC | 3 cut(s) | 495, 1079, 1381 |
| TseI | GCWGC | 4 cut(s) | 596, 743, 1093, 1163 |
| Tsp45I | GTSAC | 3 cut(s) | 495, 1079, 1381 |
| TspDTI | ATGAA | 5 cut(s) | 50, 275, 805, 1022, 1196 |
| TspGWI | ACGGA | 3 cut(s) | 114, 227, 414 |
| TspMI | CCCGGG | 1 cut(s) | 319 |
| TspRI | CASTG | 1 cut(s) | 814 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1172 |
| Vha464I | CTTAAG | 1 cut(s) | 1115 |
| VpaK11BI | GGWCC | 1 cut(s) | 248 |
| VspI | ATTAAT | 3 cut(s) | 552, 738, 1110 |
| XapI | RAATTY | 1 cut(s) | 651 |
| XceI | RCATGY | 3 cut(s) | 306, 370, 1480 |
| XmaI | CCCGGG | 1 cut(s) | 319 |
| XmiI | GTMKAC | 2 cut(s) | 852, 1176 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1261 |
| ZraI | GACGTC | 1 cut(s) | 76 |
| ZrmI | AGTACT | 1 cut(s) | 387 |