Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 629 |
| Acc36I | ACCTGC | 1 cut(s) | 1343 |
| AccB1I | GGYRCC | 1 cut(s) | 182 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1552 |
| AccBSI | CCGCTC | 1 cut(s) | 1280 |
| AccI | GTMKAC | 1 cut(s) | 984 |
| AccII | CGCG | 1 cut(s) | 606 |
| AccIII | TCCGGA | 1 cut(s) | 1157 |
| AciI | CCGC | 7 cut(s) | 373, 606, 636, 779, 812, 942, 1280 |
| AclWI | GGATC | 3 cut(s) | 1218, 1267, 1563 |
| AcoI | YGGCCR | 3 cut(s) | 637, 1092, 1105 |
| AcuI | CTGAAG | 1 cut(s) | 62 |
| AcyI | GRCGYC | 1 cut(s) | 626 |
| AfaI | GTAC | 4 cut(s) | 49, 296, 1046, 1310 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 79, 80, 1385, 1552 |
| AflIII | ACRYGT | 2 cut(s) | 434, 1305 |
| AgeI | ACCGGT | 1 cut(s) | 185 |
| AgsI | TTSAA | 2 cut(s) | 671, 1588 |
| AjnI | CCWGG | 2 cut(s) | 451, 1107 |
| AluBI | AGCT | 5 cut(s) | 925, 1114, 1168, 1494, 1602 |
| AluI | AGCT | 5 cut(s) | 925, 1114, 1168, 1494, 1602 |
| Alw21I | GWGCWC | 1 cut(s) | 1505 |
| Alw26I | GTCTC | 4 cut(s) | 633, 767, 1214, 1384 |
| AlwI | GGATC | 3 cut(s) | 1218, 1267, 1563 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1552 |
| Ama87I | CYCGRG | 1 cut(s) | 1027 |
| Aor13HI | TCCGGA | 1 cut(s) | 1157 |
| AoxI | GGCC | 5 cut(s) | 637, 1024, 1092, 1105, 1376 |
| ApeKI | GCWGC | 3 cut(s) | 875, 990, 1426 |
| AseI | ATTAAT | 1 cut(s) | 870 |
| AsiGI | ACCGGT | 1 cut(s) | 185 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 207 |
| AspLEI | GCGC | 2 cut(s) | 880, 1334 |
| AspS9I | GGNCC | 5 cut(s) | 96, 380, 825, 1025, 1376 |
| AsuC2I | CCSGG | 5 cut(s) | 74, 542, 977, 1028, 1029 |
| AsuHPI | GGTGA | 2 cut(s) | 478, 999 |
| AsuII | TTCGAA | 1 cut(s) | 68 |
| AvaI | CYCGRG | 1 cut(s) | 1027 |
| AvaII | GGWCC | 3 cut(s) | 96, 380, 825 |
| BaeGI | GKGCMC | 3 cut(s) | 362, 1077, 1465 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 529, 562 |
| BalI | TGGCCA | 1 cut(s) | 1107 |
| BanI | GGYRCC | 1 cut(s) | 182 |
| BauI | CACGAG | 1 cut(s) | 630 |
| Bbv12I | GWGCWC | 1 cut(s) | 1505 |
| BbvI | GCAGC | 3 cut(s) | 887, 977, 1438 |
| BccI | CCATC | 5 cut(s) | 145, 524, 704, 803, 922 |
| BceAI | ACGGC | 3 cut(s) | 830, 1319, 1481 |
| BciT130I | CCWGG | 2 cut(s) | 453, 1109 |
| BciVI | GTATCC | 1 cut(s) | 530 |
| BcnI | CCSGG | 5 cut(s) | 74, 542, 977, 1028, 1029 |
| BcoDI | GTCTC | 4 cut(s) | 633, 767, 1214, 1384 |
| BfaI | CTAG | 2 cut(s) | 87, 1019 |
| BfmI | CTRYAG | 1 cut(s) | 937 |
| BfoI | RGCGCY | 1 cut(s) | 1335 |
| BfuAI | ACCTGC | 1 cut(s) | 1343 |
| BfuI | GTATCC | 1 cut(s) | 530 |
| BisI | GCNGC | 4 cut(s) | 637, 876, 991, 1427 |
| BlsI | GCNGC | 4 cut(s) | 638, 877, 992, 1428 |
| BmcAI | AGTACT | 1 cut(s) | 296 |
| Bme1390I | CCNGG | 7 cut(s) | 74, 453, 542, 977, 1028, 1029, 1109 |
| Bme18I | GGWCC | 3 cut(s) | 96, 380, 825 |
| BmeT110I | CYCGRG | 1 cut(s) | 1027 |
| BmgT120I | GGNCC | 5 cut(s) | 96, 380, 825, 1025, 1376 |
| BmiI | GGNNCC | 4 cut(s) | 184, 715, 1284, 1469 |
| BmrFI | CCNGG | 7 cut(s) | 74, 453, 542, 977, 1028, 1029, 1109 |
| BmrI | ACTGGG | 1 cut(s) | 1540 |
| BmsI | GCATC | 2 cut(s) | 737, 1304 |
| BmuI | ACTGGG | 1 cut(s) | 1540 |
| Bpu14I | TTCGAA | 1 cut(s) | 68 |
| BpuEI | CTTGAG | 1 cut(s) | 1519 |
| BpuMI | CCSGG | 5 cut(s) | 74, 542, 977, 1028, 1029 |
| Bsa29I | ATCGAT | 2 cut(s) | 798, 819 |
| BsaAI | YACGTR | 2 cut(s) | 557, 1266 |
| BsaHI | GRCGYC | 1 cut(s) | 626 |
| BsaI | GGTCTC | 1 cut(s) | 767 |
| BsaJI | CCNNGG | 6 cut(s) | 353, 451, 541, 619, 1027, 1339 |
| BsaWI | WCCGGW | 2 cut(s) | 185, 1157 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 79, 80, 1385, 1552 |
| Bse118I | RCCGGY | 1 cut(s) | 185 |
| Bse1I | ACTGG | 1 cut(s) | 1546 |
| Bse3DI | GCAATG | 4 cut(s) | 257, 807, 1297, 1353 |
| BseAI | TCCGGA | 1 cut(s) | 1157 |
| BseBI | CCWGG | 2 cut(s) | 453, 1109 |
| BseCI | ATCGAT | 2 cut(s) | 798, 819 |
| BseDI | CCNNGG | 6 cut(s) | 353, 451, 541, 619, 1027, 1339 |
| BseGI | GGATG | 4 cut(s) | 316, 415, 594, 985 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 79, 80, 1385, 1552 |
| BseMI | GCAATG | 4 cut(s) | 257, 807, 1297, 1353 |
| BseMII | CTCAG | 2 cut(s) | 294, 1113 |
| BseNI | ACTGG | 1 cut(s) | 1546 |
| BseRI | GAGGAG | 2 cut(s) | 720, 866 |
| BseSI | GKGCMC | 3 cut(s) | 362, 1077, 1465 |
| BseXI | GCAGC | 3 cut(s) | 887, 977, 1438 |
| BsgI | GTGCAG | 1 cut(s) | 1297 |
| Bsh1236I | CGCG | 1 cut(s) | 606 |
| BshFI | GGCC | 5 cut(s) | 639, 1026, 1094, 1107, 1378 |
| BshNI | GGYRCC | 1 cut(s) | 182 |
| BshTI | ACCGGT | 1 cut(s) | 185 |
| BshVI | ATCGAT | 2 cut(s) | 798, 819 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1505 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1027 |
| BsiSI | CCGG | 7 cut(s) | 74, 186, 370, 541, 976, 1028, 1158 |
| BslFI | GGGAC | 1 cut(s) | 637 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 79, 80, 1385, 1552 |
| BsmAI | GTCTC | 4 cut(s) | 633, 767, 1214, 1384 |
| BsmBI | CGTCTC | 2 cut(s) | 633, 1214 |
| BsmFI | GGGAC | 1 cut(s) | 637 |
| BsmI | GAATGC | 3 cut(s) | 4, 666, 1128 |
| BsnI | GGCC | 5 cut(s) | 639, 1026, 1094, 1107, 1378 |
| Bso31I | GGTCTC | 1 cut(s) | 767 |
| BsoBI | CYCGRG | 1 cut(s) | 1027 |
| Bsp119I | TTCGAA | 1 cut(s) | 68 |
| Bsp1286I | GDGCHC | 4 cut(s) | 362, 1077, 1465, 1505 |
| Bsp13I | TCCGGA | 1 cut(s) | 1157 |
| Bsp143I | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| Bsp19I | CCATGG | 1 cut(s) | 1339 |
| BspACI | CCGC | 7 cut(s) | 373, 606, 636, 779, 812, 942, 1280 |
| BspANI | GGCC | 5 cut(s) | 639, 1026, 1094, 1107, 1378 |
| BspCNI | CTCAG | 2 cut(s) | 293, 1114 |
| BspDI | ATCGAT | 2 cut(s) | 798, 819 |
| BspEI | TCCGGA | 1 cut(s) | 1157 |
| BspFNI | CGCG | 1 cut(s) | 606 |
| BspLI | GGNNCC | 4 cut(s) | 184, 715, 1284, 1469 |
| BspMI | ACCTGC | 1 cut(s) | 1343 |
| BspPI | GGATC | 3 cut(s) | 1218, 1267, 1563 |
| BspT104I | TTCGAA | 1 cut(s) | 68 |
| BspT107I | GGYRCC | 1 cut(s) | 182 |
| BspTNI | GGTCTC | 1 cut(s) | 767 |
| BsrBI | CCGCTC | 1 cut(s) | 1280 |
| BsrDI | GCAATG | 4 cut(s) | 257, 807, 1297, 1353 |
| BsrFI | RCCGGY | 1 cut(s) | 185 |
| BsrI | ACTGG | 1 cut(s) | 1546 |
| BssAI | RCCGGY | 1 cut(s) | 185 |
| BssECI | CCNNGG | 6 cut(s) | 353, 451, 541, 619, 1027, 1339 |
| BssMI | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| BssNI | GRCGYC | 1 cut(s) | 626 |
| BssSI | CACGAG | 1 cut(s) | 630 |
| BssT1I | CCWWGG | 3 cut(s) | 353, 619, 1339 |
| Bst2BI | CACGAG | 1 cut(s) | 630 |
| Bst2UI | CCWGG | 2 cut(s) | 453, 1109 |
| Bst4CI | ACNGT | 5 cut(s) | 17, 319, 350, 466, 1372 |
| BstACI | GRCGYC | 1 cut(s) | 626 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 872, 968 |
| BstBAI | YACGTR | 2 cut(s) | 557, 1266 |
| BstBI | TTCGAA | 1 cut(s) | 68 |
| BstC8I | GCNNGC | 7 cut(s) | 375, 491, 960, 969, 973, 1105, 1316 |
| BstDEI | CTNAG | 3 cut(s) | 280, 579, 1122 |
| BstDSI | CCRYGG | 1 cut(s) | 1339 |
| BstF5I | GGATG | 4 cut(s) | 316, 415, 594, 985 |
| BstFNI | CGCG | 1 cut(s) | 606 |
| BstH2I | RGCGCY | 1 cut(s) | 1335 |
| BstHHI | GCGC | 2 cut(s) | 880, 1334 |
| BstKTI | GATC | 7 cut(s) | 333, 657, 676, 1206, 1213, 1275, 1558 |
| BstMAI | GTCTC | 4 cut(s) | 633, 767, 1214, 1384 |
| BstMBI | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 863, 872, 959, 964, 968, 1100, 1331, 1500 |
| BstNI | CCWGG | 2 cut(s) | 453, 1109 |
| BstNSI | RCATGY | 3 cut(s) | 438, 884, 962 |
| BstSCI | CCNGG | 7 cut(s) | 72, 451, 540, 975, 1026, 1027, 1107 |
| BstSFI | CTRYAG | 1 cut(s) | 937 |
| BstSLI | GKGCMC | 3 cut(s) | 362, 1077, 1465 |
| BstSNI | TACGTA | 1 cut(s) | 1266 |
| BstUI | CGCG | 1 cut(s) | 606 |
| BstV1I | GCAGC | 3 cut(s) | 887, 977, 1438 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 503, 1181 |
| Bsu15I | ATCGAT | 2 cut(s) | 798, 819 |
| BsuI | GTATCC | 1 cut(s) | 530 |
| BsuRI | GGCC | 5 cut(s) | 639, 1026, 1094, 1107, 1378 |
| BsuTUI | ATCGAT | 2 cut(s) | 798, 819 |
| BtgI | CCRYGG | 1 cut(s) | 1339 |
| BtsCI | GGATG | 4 cut(s) | 316, 415, 594, 985 |
| BtsI | GCAGTG | 1 cut(s) | 840 |
| BtsIMutI | CAGTG | 3 cut(s) | 38, 315, 840 |
| BveI | ACCTGC | 1 cut(s) | 1343 |
| Cac8I | GCNNGC | 7 cut(s) | 375, 491, 960, 969, 973, 1105, 1316 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1552 |
| CfoI | GCGC | 2 cut(s) | 880, 1334 |
| Cfr10I | RCCGGY | 1 cut(s) | 185 |
| Cfr13I | GGNCC | 5 cut(s) | 96, 380, 825, 1025, 1376 |
| Cfr9I | CCCGGG | 1 cut(s) | 1027 |
| ClaI | ATCGAT | 2 cut(s) | 798, 819 |
| CseI | GACGC | 1 cut(s) | 1160 |
| Csp6I | GTAC | 4 cut(s) | 48, 295, 1045, 1309 |
| CspAI | ACCGGT | 1 cut(s) | 185 |
| CviAII | CATG | 9 cut(s) | 160, 419, 435, 881, 959, 1078, 1090, 1138, 1340 |
| CviQI | GTAC | 4 cut(s) | 48, 295, 1045, 1309 |
| DdeI | CTNAG | 3 cut(s) | 280, 579, 1122 |
| DpnI | GATC | 7 cut(s) | 332, 656, 675, 1205, 1212, 1274, 1557 |
| DpnII | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| EaeI | YGGCCR | 3 cut(s) | 637, 1092, 1105 |
| Eco105I | TACGTA | 1 cut(s) | 1266 |
| Eco130I | CCWWGG | 3 cut(s) | 353, 619, 1339 |
| Eco31I | GGTCTC | 1 cut(s) | 767 |
| Eco47I | GGWCC | 3 cut(s) | 96, 380, 825 |
| Eco57I | CTGAAG | 1 cut(s) | 62 |
| Eco88I | CYCGRG | 1 cut(s) | 1027 |
| EcoO109I | RGGNCCY | 2 cut(s) | 380, 825 |
| EcoRII | CCWGG | 2 cut(s) | 451, 1107 |
| EcoT14I | CCWWGG | 3 cut(s) | 353, 619, 1339 |
| EcoT22I | ATGCAT | 3 cut(s) | 6, 960, 1297 |
| ErhI | CCWWGG | 3 cut(s) | 353, 619, 1339 |
| Esp3I | CGTCTC | 2 cut(s) | 633, 1214 |
| FaeI | CATG | 9 cut(s) | 163, 422, 438, 884, 962, 1081, 1093, 1141, 1343 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 281, 313 |
| FaqI | GGGAC | 1 cut(s) | 637 |
| FatI | CATG | 9 cut(s) | 159, 418, 434, 880, 958, 1077, 1089, 1137, 1339 |
| FauI | CCCGC | 3 cut(s) | 366, 772, 935 |
| FauNDI | CATATG | 1 cut(s) | 498 |
| FblI | GTMKAC | 1 cut(s) | 984 |
| Fnu4HI | GCNGC | 4 cut(s) | 637, 876, 991, 1427 |
| FokI | GGATG | 4 cut(s) | 323, 422, 581, 992 |
| Fsp4HI | GCNGC | 4 cut(s) | 637, 876, 991, 1427 |
| FspBI | CTAG | 2 cut(s) | 87, 1019 |
| GlaI | GCGC | 2 cut(s) | 879, 1333 |
| GluI | GCNGC | 4 cut(s) | 637, 876, 991, 1427 |
| HaeII | RGCGCY | 1 cut(s) | 1335 |
| HaeIII | GGCC | 5 cut(s) | 639, 1026, 1094, 1107, 1378 |
| HapII | CCGG | 7 cut(s) | 74, 186, 370, 541, 976, 1028, 1158 |
| HgaI | GACGC | 1 cut(s) | 1160 |
| HhaI | GCGC | 2 cut(s) | 880, 1334 |
| Hin1I | GRCGYC | 1 cut(s) | 626 |
| Hin1II | CATG | 9 cut(s) | 163, 422, 438, 884, 962, 1081, 1093, 1141, 1343 |
| Hin6I | GCGC | 2 cut(s) | 878, 1332 |
| HinP1I | GCGC | 2 cut(s) | 878, 1332 |
| HincII | GTYRAC | 1 cut(s) | 661 |
| HindII | GTYRAC | 1 cut(s) | 661 |
| HindIII | AAGCTT | 1 cut(s) | 1166 |
| HpaII | CCGG | 7 cut(s) | 74, 186, 370, 541, 976, 1028, 1158 |
| HphI | GGTGA | 2 cut(s) | 478, 999 |
| Hpy166II | GTNNAC | 8 cut(s) | 13, 340, 589, 661, 694, 985, 1034, 1309 |
| Hpy188I | TCNGA | 9 cut(s) | 61, 123, 223, 409, 524, 892, 1123, 1277, 1300 |
| Hpy188III | TCNNGA | 8 cut(s) | 87, 200, 511, 671, 1001, 1158, 1498, 1559 |
| Hpy8I | GTNNAC | 8 cut(s) | 13, 340, 589, 661, 694, 985, 1034, 1309 |
| Hpy99I | CGWCG | 1 cut(s) | 759 |
| HpyAV | CCTTC | 4 cut(s) | 37, 1016, 1091, 1522 |
| HpyCH4III | ACNGT | 5 cut(s) | 17, 319, 350, 466, 1372 |
| HpyCH4IV | ACGT | 5 cut(s) | 556, 626, 862, 1265, 1305 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 863, 872, 959, 964, 968, 1100, 1331, 1500 |
| HpyF3I | CTNAG | 3 cut(s) | 280, 579, 1122 |
| HpySE526I | ACGT | 5 cut(s) | 556, 626, 862, 1265, 1305 |
| Hsp92I | GRCGYC | 1 cut(s) | 626 |
| Hsp92II | CATG | 9 cut(s) | 163, 422, 438, 884, 962, 1081, 1093, 1141, 1343 |
| HspAI | GCGC | 2 cut(s) | 878, 1332 |
| Kpn2I | TCCGGA | 1 cut(s) | 1157 |
| Kzo9I | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| LmnI | GCTCC | 4 cut(s) | 1111, 1323, 1343, 1473 |
| Lsp1109I | GCAGC | 3 cut(s) | 887, 977, 1438 |
| LweI | GCATC | 2 cut(s) | 737, 1304 |
| MaeI | CTAG | 2 cut(s) | 87, 1019 |
| MaeII | ACGT | 5 cut(s) | 556, 626, 862, 1265, 1305 |
| MaeIII | GTNAC | 1 cut(s) | 430 |
| MalI | GATC | 7 cut(s) | 332, 656, 675, 1205, 1212, 1274, 1557 |
| MbiI | CCGCTC | 1 cut(s) | 1280 |
| MboI | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| MboII | GAAGA | 5 cut(s) | 12, 215, 1213, 1467, 1544 |
| MhlI | GDGCHC | 4 cut(s) | 362, 1077, 1465, 1505 |
| MlsI | TGGCCA | 1 cut(s) | 1107 |
| MluCI | AATT | 6 cut(s) | 525, 699, 747, 867, 996, 1612 |
| MluNI | TGGCCA | 1 cut(s) | 1107 |
| MlyI | GAGTC | 4 cut(s) | 413, 450, 568, 1053 |
| MmeI | TCCRAC | 2 cut(s) | 387, 1162 |
| Mox20I | TGGCCA | 1 cut(s) | 1107 |
| Mph1103I | ATGCAT | 3 cut(s) | 6, 960, 1297 |
| MroI | TCCGGA | 1 cut(s) | 1157 |
| MroXI | GAANNNNTTC | 1 cut(s) | 207 |
| MscI | TGGCCA | 1 cut(s) | 1107 |
| MseI | TTAA | 4 cut(s) | 303, 750, 870, 995 |
| MslI | CAYNNNNRTG | 3 cut(s) | 501, 1088, 1563 |
| Msp20I | TGGCCA | 1 cut(s) | 1107 |
| MspA1I | CMGCKG | 1 cut(s) | 942 |
| MspI | CCGG | 7 cut(s) | 74, 186, 370, 541, 976, 1028, 1158 |
| MspR9I | CCNGG | 7 cut(s) | 74, 453, 542, 977, 1028, 1029, 1109 |
| Mva1269I | GAATGC | 3 cut(s) | 4, 666, 1128 |
| MvaI | CCWGG | 2 cut(s) | 453, 1109 |
| MvnI | CGCG | 1 cut(s) | 606 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 863, 872, 959, 964, 968, 1100, 1331, 1500 |
| NciI | CCSGG | 5 cut(s) | 74, 542, 977, 1028, 1029 |
| NcoI | CCATGG | 1 cut(s) | 1339 |
| NdeI | CATATG | 1 cut(s) | 498 |
| NdeII | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| NlaIII | CATG | 9 cut(s) | 163, 422, 438, 884, 962, 1081, 1093, 1141, 1343 |
| NlaIV | GGNNCC | 4 cut(s) | 184, 715, 1284, 1469 |
| NmeAIII | GCCGAG | 1 cut(s) | 1515 |
| NmuCI | GTSAC | 1 cut(s) | 430 |
| NsiI | ATGCAT | 3 cut(s) | 6, 960, 1297 |
| NspI | RCATGY | 3 cut(s) | 438, 884, 962 |
| NspV | TTCGAA | 1 cut(s) | 68 |
| PaeI | GCATGC | 1 cut(s) | 962 |
| PciI | ACATGT | 1 cut(s) | 434 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 65 |
| PctI | GAATGC | 3 cut(s) | 4, 666, 1128 |
| PdmI | GAANNNNTTC | 1 cut(s) | 207 |
| PfeI | GAWTC | 6 cut(s) | 565, 681, 783, 887, 1004, 1416 |
| PflFI | GACNNNGTC | 2 cut(s) | 384, 759 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1552 |
| PinAI | ACCGGT | 1 cut(s) | 185 |
| PkrI | GCNGC | 4 cut(s) | 638, 877, 992, 1428 |
| PleI | GAGTC | 4 cut(s) | 412, 450, 568, 1053 |
| PpsI | GAGTC | 4 cut(s) | 412, 450, 568, 1053 |
| Ppu21I | YACGTR | 2 cut(s) | 557, 1266 |
| PpuMI | RGGWCCY | 2 cut(s) | 380, 825 |
| PscI | ACATGT | 1 cut(s) | 434 |
| PshBI | ATTAAT | 1 cut(s) | 870 |
| Psp5II | RGGWCCY | 2 cut(s) | 380, 825 |
| Psp6I | CCWGG | 2 cut(s) | 451, 1107 |
| PspGI | CCWGG | 2 cut(s) | 451, 1107 |
| PspN4I | GGNNCC | 4 cut(s) | 184, 715, 1284, 1469 |
| PspPI | GGNCC | 5 cut(s) | 96, 380, 825, 1025, 1376 |
| PspPPI | RGGWCCY | 2 cut(s) | 380, 825 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1552 |
| PsyI | GACNNNGTC | 2 cut(s) | 384, 759 |
| RsaI | GTAC | 4 cut(s) | 49, 296, 1046, 1310 |
| RsaNI | GTAC | 4 cut(s) | 48, 295, 1045, 1309 |
| RseI | CAYNNNNRTG | 3 cut(s) | 501, 1088, 1563 |
| SaqAI | TTAA | 4 cut(s) | 303, 750, 870, 995 |
| SatI | GCNGC | 4 cut(s) | 637, 876, 991, 1427 |
| Sau3AI | GATC | 7 cut(s) | 330, 654, 673, 1203, 1210, 1272, 1555 |
| Sau96I | GGNCC | 5 cut(s) | 96, 380, 825, 1025, 1376 |
| ScaI | AGTACT | 1 cut(s) | 296 |
| SchI | GAGTC | 4 cut(s) | 413, 450, 568, 1053 |
| ScrFI | CCNGG | 7 cut(s) | 74, 453, 542, 977, 1028, 1029, 1109 |
| SduI | GDGCHC | 4 cut(s) | 362, 1077, 1465, 1505 |
| SfaNI | GCATC | 2 cut(s) | 737, 1304 |
| SfcI | CTRYAG | 1 cut(s) | 937 |
| SfuI | TTCGAA | 1 cut(s) | 68 |
| SgrAI | CRCCGGYG | 1 cut(s) | 185 |
| SinI | GGWCC | 3 cut(s) | 96, 380, 825 |
| SmaI | CCCGGG | 1 cut(s) | 1029 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 501, 1088, 1563 |
| SmlI | CTYRAG | 1 cut(s) | 1498 |
| SmoI | CTYRAG | 1 cut(s) | 1498 |
| SnaBI | TACGTA | 1 cut(s) | 1266 |
| SphI | GCATGC | 1 cut(s) | 962 |
| Sse9I | AATT | 6 cut(s) | 525, 699, 747, 867, 996, 1612 |
| SsiI | CCGC | 7 cut(s) | 373, 606, 636, 779, 812, 942, 1280 |
| SspMI | CTAG | 2 cut(s) | 87, 1019 |
| StyD4I | CCNGG | 7 cut(s) | 72, 451, 540, 975, 1026, 1027, 1107 |
| StyI | CCWWGG | 3 cut(s) | 353, 619, 1339 |
| TaaI | ACNGT | 5 cut(s) | 17, 319, 350, 466, 1372 |
| TaiI | ACGT | 5 cut(s) | 559, 629, 865, 1268, 1308 |
| TaqI | TCGA | 8 cut(s) | 68, 201, 402, 798, 819, 885, 1478, 1518 |
| TasI | AATT | 6 cut(s) | 525, 699, 747, 867, 996, 1612 |
| TatI | WGTACW | 3 cut(s) | 294, 1044, 1308 |
| TauI | GCSGC | 1 cut(s) | 639 |
| TfiI | GAWTC | 6 cut(s) | 565, 681, 783, 887, 1004, 1416 |
| Tru1I | TTAA | 4 cut(s) | 303, 750, 870, 995 |
| Tru9I | TTAA | 4 cut(s) | 303, 750, 870, 995 |
| TscAI | CASTG | 3 cut(s) | 45, 322, 847 |
| TseFI | GTSAC | 1 cut(s) | 430 |
| TseI | GCWGC | 3 cut(s) | 875, 990, 1426 |
| Tsp45I | GTSAC | 1 cut(s) | 430 |
| TspDTI | ATGAA | 3 cut(s) | 407, 1121, 1400 |
| TspMI | CCCGGG | 1 cut(s) | 1027 |
| TspRI | CASTG | 3 cut(s) | 45, 322, 847 |
| Tth111I | GACNNNGTC | 2 cut(s) | 384, 759 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1552 |
| VpaK11BI | GGWCC | 3 cut(s) | 96, 380, 825 |
| VspI | ATTAAT | 1 cut(s) | 870 |
| XbaI | TCTAGA | 1 cut(s) | 86 |
| XceI | RCATGY | 3 cut(s) | 438, 884, 962 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 605 |
| XmaI | CCCGGG | 1 cut(s) | 1027 |
| XmiI | GTMKAC | 1 cut(s) | 984 |
| XmnI | GAANNNNTTC | 1 cut(s) | 207 |
| XspI | CTAG | 2 cut(s) | 87, 1019 |
| ZraI | GACGTC | 1 cut(s) | 627 |
| ZrmI | AGTACT | 1 cut(s) | 296 |
| Zsp2I | ATGCAT | 3 cut(s) | 6, 960, 1297 |