Rh6BG436300

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000762
Physical Location & Seq
Reverse (-)
65587056 .. 65591693
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG436300.1

Sequence Viewer

Length: 1134 bp
ATGGCGGTTTCCGGTGATCTGAAGATGAAGCTCGTCGAAGGCGACGCCGGTTACGTCCTCGAAGATGTGCCTCATCTCGCCGATTACTTGTCCGATCTTCCTACTTATCCCAATCCACTGCAAATCAATCCTGCTTACTCTGTAGTCAAGCAATATTTTGTTAACCAAGATGACACTGTTGCTCAAAAGATTGTTGTGCACAAGGATAGTCCAAGAGGGACACACCTTCGGCGAGCAGGACCACGCCAAAAGGTGTATTTTGAGGCGGATGAAGTGCATGCCTGTGTTGTGACTTGTGGTGGTTTGTGCCCTGGGCTCAACACGGTGATCAGGGAAATTGTCTGTGGACTTTATCACATGTATGGTGTCACCAGGGTTCTTGGAATAAATGGAGGATATAGTGGTTTCTATTCCCGAAATACTATTGAGTTGACACCAAGGGTTGTGAATGACATCCACAAACGTGGTGGTACTATCCTTGGGACATCACGAGGAGGCTATGATACCTCGAAGATAGTTGACAGCATTCATGATCGTGGAATTAACCATGTTTACATAATTGGAGGTGATGGAACTCAAAAAGGAGCATCTGTGATATTTGAGGAAGTTAGACGCCGTGGCCTCAAAGTTTCTGTTGTAGGAGTTCCTAAAACAATAGACAATGACATACCGGTTATAGATAGATCCTTTGGCTTTGATACTGCGGTTGAGGAGGCCCAGCGTGCCATATGTGCAGCTCATGTGGAATCTGAAAGTATTGAGCATGGTATTGGTCTTGTGAAGCTAATGGGACGCTACAGCGGTTTCATAGCTATGTATGCTACACTTGCCAGTCGAGATGTGGACTGTTGTTTGATTCCAGAGTCCCCCTTCTTTCTTGAAGGACGAGGTGGACTTTTTGAGTTCATTGAAAGACGACTCAAAGAAAATGGACATATGGTTATTGTGTTGGCTGAGGGAGCAGGACAGGATCTTCTTTCAGAGAGCTTGCAATCCATGAACCAGCAAGATGCTTCAGGAAACAAGCTACTTCAAGATGTTGGGTTATGGATATCTCAGAGGATCAAGGTATTACTTGCAATCCCTTATATTTTCTTCTTTGCATCACATGATATCATGAAAGAGTCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

377

Amino Acids

41.5

Weight (kDa)

5.96

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 95 - 334 2.7e-57 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 266, 704, 801
AclWI GGATC 3 cut(s) 678, 978, 1070
AcuI CTGAAG 2 cut(s) 41, 999
AcyI GRCGYC 2 cut(s) 45, 613
AfaI GTAC 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 357
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 3 cut(s) 881, 911, 1034
AjnI CCWGG 2 cut(s) 310, 371
AleI CACNNNNGTG 1 cut(s) 462
AluBI AGCT 6 cut(s) 31, 737, 784, 812, 987, 1027
AluI AGCT 6 cut(s) 31, 737, 784, 812, 987, 1027
Alw21I GWGCWC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 197
AlwI GGATC 3 cut(s) 678, 978, 1070
AoxI GGCC 2 cut(s) 619, 714
ApaLI GTGCAC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 734
AsiGI ACCGGT 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 239, 715
AsuHPI GGTGA 4 cut(s) 26, 337, 361, 578
AvaII GGWCC 1 cut(s) 239
BaeGI GKGCMC 2 cut(s) 201, 311
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BanII GRGCYC 1 cut(s) 318
BauI CACGAG 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 1 cut(s) 746
BccI CCATC 1 cut(s) 563
BceAI ACGGC 1 cut(s) 600
BciT130I CCWGG 2 cut(s) 312, 373
BclI TGATCA 1 cut(s) 327
BfmI CTRYAG 2 cut(s) 141, 796
BisI GCNGC 1 cut(s) 735
BlsI GCNGC 1 cut(s) 736
Bme1390I CCNGG 2 cut(s) 312, 373
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 239, 715
BmrFI CCNGG 2 cut(s) 312, 373
BmsI GCATC 3 cut(s) 596, 1000, 1112
Bpu10I CCTNAGC 1 cut(s) 954
BsaHI GRCGYC 2 cut(s) 45, 613
BsaJI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BsaWI WCCGGW 2 cut(s) 11, 670
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bse118I RCCGGY 2 cut(s) 47, 670
Bse1I ACTGG 1 cut(s) 831
BseBI CCWGG 2 cut(s) 312, 373
BseDI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BseGI GGATG 2 cut(s) 274, 453
BseMII CTCAG 2 cut(s) 945, 1070
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 2 cut(s) 507, 725
BseSI GKGCMC 2 cut(s) 201, 311
BseXI GCAGC 1 cut(s) 746
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 753
BshFI GGCC 2 cut(s) 621, 716
BshTI ACCGGT 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 3 cut(s) 12, 48, 671
BslFI GGGAC 4 cut(s) 232, 496, 804, 850
BsmFI GGGAC 4 cut(s) 232, 496, 804, 850
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 2 cut(s) 621, 716
Bsp1286I GDGCHC 3 cut(s) 201, 311, 318
Bsp143I GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
BspACI CCGC 4 cut(s) 5, 266, 704, 801
BspANI GGCC 2 cut(s) 621, 716
BspCNI CTCAG 2 cut(s) 946, 1069
BspHI TCATGA 2 cut(s) 529, 1116
BspPI GGATC 3 cut(s) 678, 978, 1070
BsrFI RCCGGY 2 cut(s) 47, 670
BsrI ACTGG 1 cut(s) 831
BssAI RCCGGY 2 cut(s) 47, 670
BssECI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BssMI GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
BssNI GRCGYC 2 cut(s) 45, 613
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 437, 478
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 312, 373
Bst4CI ACNGT 3 cut(s) 178, 325, 848
BstACI GRCGYC 2 cut(s) 45, 613
BstC8I GCNNGC 4 cut(s) 234, 279, 723, 989
BstDEI CTNAG 2 cut(s) 954, 1056
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 2 cut(s) 274, 453
BstKTI GATC 7 cut(s) 19, 97, 330, 535, 686, 973, 1065
BstMBI GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
BstMWI GCNNNNNNNGC 5 cut(s) 722, 731, 818, 827, 959
BstNI CCWGG 2 cut(s) 312, 373
BstNSI RCATGY 2 cut(s) 281, 361
BstSCI CCNGG 2 cut(s) 310, 371
BstSFI CTRYAG 2 cut(s) 141, 796
BstSLI GKGCMC 2 cut(s) 201, 311
BstV1I GCAGC 1 cut(s) 746
BstX2I RGATCY 2 cut(s) 683, 970
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 2 cut(s) 683, 970
BsuRI GGCC 2 cut(s) 621, 716
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 2 cut(s) 274, 453
BtsI GCAGTG 1 cut(s) 116
BtsIMutI CAGTG 2 cut(s) 116, 174
Cac8I GCNNGC 4 cut(s) 234, 279, 723, 989
CciI TCATGA 2 cut(s) 529, 1116
Cfr10I RCCGGY 2 cut(s) 47, 670
Cfr13I GGNCC 2 cut(s) 239, 715
CseI GACGC 3 cut(s) 53, 621, 801
Csp6I GTAC 1 cut(s) 471
CspAI ACCGGT 1 cut(s) 670
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 9 cut(s) 278, 358, 530, 548, 740, 764, 997, 1109, 1117
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 2 cut(s) 954, 1056
DpnI GATC 7 cut(s) 18, 96, 329, 534, 685, 972, 1064
DpnII GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 437, 478
Eco24I GRGCYC 1 cut(s) 318
Eco32I GATATC 2 cut(s) 1053, 1114
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 41, 999
EcoRII CCWGG 2 cut(s) 310, 371
EcoRV GATATC 2 cut(s) 1053, 1114
EcoT14I CCWWGG 2 cut(s) 437, 478
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 437, 478
FaeI CATG 9 cut(s) 281, 361, 533, 551, 743, 767, 1000, 1112, 1120
FaqI GGGAC 4 cut(s) 232, 496, 804, 850
FatI CATG 9 cut(s) 277, 357, 529, 547, 739, 763, 996, 1108, 1116
FauNDI CATATG 2 cut(s) 728, 936
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 1 cut(s) 735
FokI GGATG 2 cut(s) 281, 440
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 1 cut(s) 735
GluI GCNGC 1 cut(s) 735
GsaI CCCAGC 1 cut(s) 721
HaeIII GGCC 2 cut(s) 621, 716
HapII CCGG 3 cut(s) 12, 48, 671
HgaI GACGC 3 cut(s) 53, 621, 801
Hin1I GRCGYC 2 cut(s) 45, 613
Hin1II CATG 9 cut(s) 281, 361, 533, 551, 743, 767, 1000, 1112, 1120
HincII GTYRAC 3 cut(s) 163, 432, 520
HindII GTYRAC 3 cut(s) 163, 432, 520
HinfI GANTC 5 cut(s) 746, 856, 863, 918, 1124
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 3 cut(s) 12, 48, 671
HphI GGTGA 4 cut(s) 26, 337, 361, 578
Hpy166II GTNNAC 8 cut(s) 163, 199, 347, 432, 520, 553, 844, 893
Hpy188I TCNGA 5 cut(s) 21, 94, 751, 982, 1059
Hpy188III TCNNGA 9 cut(s) 414, 489, 530, 836, 860, 878, 1017, 1034, 1117
Hpy8I GTNNAC 8 cut(s) 163, 199, 347, 432, 520, 553, 844, 893
Hpy99I CGWCG 2 cut(s) 38, 47
HpyAV CCTTC 4 cut(s) 32, 236, 875, 880
HpyCH4III ACNGT 3 cut(s) 178, 325, 848
HpyCH4IV ACGT 2 cut(s) 54, 463
HpyCH4V TGCA 7 cut(s) 121, 199, 277, 734, 991, 1079, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 722, 731, 818, 827, 959
HpyF3I CTNAG 2 cut(s) 954, 1056
HpySE526I ACGT 2 cut(s) 54, 463
Hsp92I GRCGYC 2 cut(s) 45, 613
Hsp92II CATG 9 cut(s) 281, 361, 533, 551, 743, 767, 1000, 1112, 1120
Ksp22I TGATCA 1 cut(s) 327
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
LmnI GCTCC 2 cut(s) 584, 959
Lsp1109I GCAGC 1 cut(s) 746
LweI GCATC 3 cut(s) 596, 1000, 1112
MaeII ACGT 2 cut(s) 54, 463
MaeIII GTNAC 3 cut(s) 50, 289, 367
MalI GATC 7 cut(s) 18, 96, 329, 534, 685, 972, 1064
MboI GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
MboII GAAGA 6 cut(s) 34, 74, 89, 523, 965, 1087
MflI RGATCY 2 cut(s) 683, 970
MhlI GDGCHC 3 cut(s) 201, 311, 318
MluCI AATT 3 cut(s) 336, 540, 558
MlyI GAGTC 3 cut(s) 872, 912, 1133
MseI TTAA 2 cut(s) 162, 543
MslI CAYNNNNRTG 5 cut(s) 282, 360, 462, 534, 812
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 3 cut(s) 12, 48, 671
MspR9I CCNGG 2 cut(s) 312, 373
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 2 cut(s) 312, 373
MwoI GCNNNNNNNGC 5 cut(s) 722, 731, 818, 827, 959
NdeI CATATG 2 cut(s) 728, 936
NdeII GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
NlaIII CATG 9 cut(s) 281, 361, 533, 551, 743, 767, 1000, 1112, 1120
NmuCI GTSAC 2 cut(s) 289, 367
NspI RCATGY 2 cut(s) 281, 361
OliI CACNNNNGTG 1 cut(s) 462
PaeI GCATGC 1 cut(s) 281
PagI TCATGA 2 cut(s) 529, 1116
PasI CCCWGGG 1 cut(s) 311
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 51
PctI GAATGC 1 cut(s) 525
PfeI GAWTC 2 cut(s) 746, 856
PinAI ACCGGT 1 cut(s) 670
PkrI GCNGC 1 cut(s) 736
PleI GAGTC 3 cut(s) 871, 912, 1132
PpsI GAGTC 3 cut(s) 871, 912, 1132
PscI ACATGT 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 310, 371
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 2 cut(s) 310, 371
PspPI GGNCC 2 cut(s) 239, 715
PsuI RGATCY 2 cut(s) 683, 970
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 5 cut(s) 282, 360, 462, 534, 812
SaqAI TTAA 2 cut(s) 162, 543
SatI GCNGC 1 cut(s) 735
Sau3AI GATC 7 cut(s) 16, 94, 327, 532, 683, 970, 1062
Sau96I GGNCC 2 cut(s) 239, 715
SchI GAGTC 3 cut(s) 872, 912, 1133
ScrFI CCNGG 2 cut(s) 312, 373
SduI GDGCHC 3 cut(s) 201, 311, 318
SfaNI GCATC 3 cut(s) 596, 1000, 1112
SfcI CTRYAG 2 cut(s) 141, 796
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 5 cut(s) 282, 360, 462, 534, 812
SphI GCATGC 1 cut(s) 281
Sse9I AATT 3 cut(s) 336, 540, 558
SsiI CCGC 4 cut(s) 5, 266, 704, 801
SspI AATATT 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 310, 371
StyI CCWWGG 2 cut(s) 437, 478
TaaI ACNGT 3 cut(s) 178, 325, 848
TaiI ACGT 2 cut(s) 57, 466
TaqI TCGA 4 cut(s) 36, 60, 509, 835
TasI AATT 3 cut(s) 336, 540, 558
TfiI GAWTC 2 cut(s) 746, 856
Tru1I TTAA 2 cut(s) 162, 543
Tru9I TTAA 2 cut(s) 162, 543
TscAI CASTG 2 cut(s) 123, 181
TseFI GTSAC 2 cut(s) 289, 367
TseI GCWGC 1 cut(s) 734
Tsp45I GTSAC 2 cut(s) 289, 367
TspDTI ATGAA 7 cut(s) 41, 285, 518, 796, 895, 1013, 1133
TspRI CASTG 2 cut(s) 123, 181
VneI GTGCAC 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 239
XceI RCATGY 2 cut(s) 281, 361
XcmI CCANNNNNNNNNTGG 2 cut(s) 464, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.