AT4G29220

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000762
Physical Location & Seq
Reverse (-)
14403347 .. 14406592
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29220.1

Sequence Viewer

Length: 1422 bp
ATGTCATCTTCTGTTCCAAACTCGGACCGGAAGATTGTTACTGGTCCCGCCGGTTATATTCTTGAAGACGTTCCTCATTTCTCCGACGACTTTCCTGATCATCCTACATATCCAAATCCATTACAAGACAATGCAGCTTACTCTGTTGTTAAGCAGTATTTTGTCGATGAGGATGACACGGTTCCTCAAAAAATAGTAGTTCATCCTGATAGTCCTAGGGGAACGCATTTCCGTCGTGCAGGACCACGTCAAAGGGTTTACTTTGAGTCGGATGATGTTCTTGCTTGCATTGTTACATGTGGCGGTCTATGTCCCGGGCTTAATACTGTGATCAGAGAGATAGTTTGTGGATTATCTTATATGTATGGTGTCAAGAGAATCCTTGGCATTGATGGAGGTTACAGAGGATTTTACGCGAGAAACACAATCCATTTGGACTTAAAAACAGTGAATGATATCCATAGAAGCGGAGGAACTATCCTCGGGACTTCAAGAGGCGGTCACAACACCACCAAGATAGTAGATAGTATTCAAGATCGCGGGATTAACCAGGTTTATATAATCGGTGGAGATGGATCACAGAAAGGAGCAGCTGCTATATTCGAGGAGATTAGGAAACGCAAGCTTAAAGTTGCGGTTGCCGGAATCCCGAAAACAATTGACAATGACATTCCTATTATCGATAGATCTTTCGGGTTTGACACAGCTGTAGAAGAGGCTCAACGTGCTATCAATGCAGCACATGTCGAAGCTACAAGCTTTGAGAATGGTATTGGTCTTGTGAAGTTAATGGGACGGTACAGCGGATTCATTGCAATGCATGCCACTCTAGCCAGCCGAGATGTGGACTGTTGCTTGATCCCGGAATCTCCATTTTTTCTTGAAGGCTCGGGCGGGCTTTTTGAATTTATCGATAAACGGTTAAAGGAGAGTGGTCATATGGTGATTGTAATTGCAGAAGGTGCGGGACAAGATCTGTTGTCTGAAAGCATGAAAGAGTCCACAACTCTCAAAGATGCCTCTGGAAACAAACTTCTTCAAGACATTGGCCTATGGATCTCCCAAAGAATCAAGGATCATTTTGCCAAGAAGATGACCCTTACCCTCAAATATATAGATCCAACCTACATGATACGAGCTGTTCCGAGCAATGCATCAGACAATGTATGCTGCACGCTTTTAGCTCAAAGCGCGGTTCATGGAGTGATGGCTGGTTACAATGGTTTCACTGTTGGCCTTGTCAATGGAAGACATACTTACATTCCCTTCAATAGGATCACGGAGAAACAGAACAAGGTGGTGATCACTGACAGAATGTGGGCGAGGCTTTTGTCTTCGACGAACCAACCGAGTTTCATGAAGCAAGCTGACAAGATCCACTCAAACCAGTTGGTTGGTGAACCAGGGACCATGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0048364 GO:0048731 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0090558 GO:0090627 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

473

Amino Acids

51.99

Weight (kDa)

7.22

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 96 - 400 3.2e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1416
AccII CGCG 3 cut(s) 416, 540, 1193
AclWI GGATC 7 cut(s) 583, 853, 1064, 1083, 1112, 1283, 1369
AcsI RAATTY 1 cut(s) 905
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 3 cut(s) 844, 1272, 1416
AflIII ACRYGT 2 cut(s) 296, 742
AgsI TTSAA 7 cut(s) 65, 492, 533, 884, 905, 1040, 1270
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 2 cut(s) 549, 1402
AluBI AGCT 9 cut(s) 137, 593, 625, 707, 752, 759, 1139, 1184, 1367
AluI AGCT 9 cut(s) 137, 593, 625, 707, 752, 759, 1139, 1184, 1367
AlwI GGATC 7 cut(s) 583, 853, 1064, 1083, 1112, 1283, 1369
Ama87I CYCGRG 3 cut(s) 314, 482, 889
AoxI GGCC 2 cut(s) 1048, 1234
ApeKI GCWGC 5 cut(s) 134, 590, 593, 737, 1170
ApoI RAATTY 1 cut(s) 905
Asp700I GAANNNNTTC 1 cut(s) 69
AspA2I CCTAGG 1 cut(s) 215
AspLEI GCGC 1 cut(s) 1193
AspS9I GGNCC 4 cut(s) 25, 44, 242, 1407
AsuC2I CCSGG 3 cut(s) 315, 316, 863
AsuHPI GGTGA 3 cut(s) 955, 1312, 1409
AvaI CYCGRG 3 cut(s) 314, 482, 889
AvaII GGWCC 4 cut(s) 25, 44, 242, 1407
AvrII CCTAGG 1 cut(s) 215
BbsI GAAGAC 3 cut(s) 72, 1255, 1326
BbvI GCAGC 5 cut(s) 146, 580, 602, 749, 1157
BccI CCATC 3 cut(s) 386, 566, 1201
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 551, 1404
BclI TGATCA 3 cut(s) 97, 330, 1302
BcnI CCSGG 3 cut(s) 315, 316, 863
BfaI CTAG 2 cut(s) 216, 830
BfmI CTRYAG 1 cut(s) 708
BglII AGATCT 2 cut(s) 686, 973
BisI GCNGC 5 cut(s) 135, 591, 594, 738, 1171
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 5 cut(s) 136, 592, 595, 739, 1172
Bme1390I CCNGG 5 cut(s) 315, 316, 551, 863, 1404
Bme18I GGWCC 4 cut(s) 25, 44, 242, 1407
BmeT110I CYCGRG 3 cut(s) 314, 482, 889
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 4 cut(s) 25, 44, 242, 1407
BmiI GGNNCC 3 cut(s) 46, 183, 1408
BmrFI CCNGG 5 cut(s) 315, 316, 551, 863, 1404
BmsI GCATC 2 cut(s) 1006, 1163
BpiI GAAGAC 3 cut(s) 72, 1255, 1326
BpuMI CCSGG 3 cut(s) 315, 316, 863
Bsa29I ATCGAT 2 cut(s) 681, 912
BsaJI CCNNGG 5 cut(s) 215, 314, 382, 481, 1403
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 844, 1272, 1416
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 2 cut(s) 46, 1387
Bse3DI GCAATG 3 cut(s) 810, 822, 1156
BseBI CCWGG 2 cut(s) 551, 1404
BseCI ATCGAT 2 cut(s) 681, 912
BseDI CCNNGG 5 cut(s) 215, 314, 382, 481, 1403
BseGI GGATG 4 cut(s) 100, 178, 202, 277
BseLI CCNNNNNNNGG 3 cut(s) 844, 1272, 1416
BseMI GCAATG 3 cut(s) 810, 822, 1156
BseNI ACTGG 2 cut(s) 46, 1387
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 5 cut(s) 146, 580, 602, 749, 1157
BsgI GTGCAG 2 cut(s) 258, 1156
Bsh1236I CGCG 3 cut(s) 416, 540, 1193
BshFI GGCC 2 cut(s) 1050, 1236
BshVI ATCGAT 2 cut(s) 681, 912
BsiHKCI CYCGRG 3 cut(s) 314, 482, 889
BsiSI CCGG 5 cut(s) 28, 51, 315, 642, 863
BslFI GGGAC 5 cut(s) 30, 297, 499, 807, 981
BslI CCNNNNNNNGG 3 cut(s) 844, 1272, 1416
BsmFI GGGAC 5 cut(s) 30, 297, 499, 807, 981
BsnI GGCC 2 cut(s) 1050, 1236
BsoBI CYCGRG 3 cut(s) 314, 482, 889
BspANI GGCC 2 cut(s) 1050, 1236
BspDI ATCGAT 2 cut(s) 681, 912
BspFNI CGCG 3 cut(s) 416, 540, 1193
BspHI TCATGA 1 cut(s) 1356
BspLI GGNNCC 3 cut(s) 46, 183, 1408
BspPI GGATC 7 cut(s) 583, 853, 1064, 1083, 1112, 1283, 1369
BsrDI GCAATG 3 cut(s) 810, 822, 1156
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 2 cut(s) 46, 1387
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 5 cut(s) 215, 314, 382, 481, 1403
BssT1I CCWWGG 2 cut(s) 215, 382
Bst2UI CCWGG 2 cut(s) 551, 1404
Bst4CI ACNGT 7 cut(s) 181, 328, 448, 798, 851, 921, 1231
Bst6I CTCTTC 1 cut(s) 708
BstAPI GCANNNNNTGC 2 cut(s) 821, 962
BstC8I GCNNGC 7 cut(s) 286, 623, 822, 835, 896, 1175, 1365
BstENI CCTNNNNNAGG 1 cut(s) 1270
BstF5I GGATG 4 cut(s) 100, 178, 202, 277
BstFNI CGCG 3 cut(s) 416, 540, 1193
BstHHI GCGC 1 cut(s) 1193
BstMWI GCNNNNNNNGC 6 cut(s) 725, 734, 821, 830, 962, 1190
BstNI CCWGG 2 cut(s) 551, 1404
BstNSI RCATGY 3 cut(s) 300, 746, 824
BstSCI CCNGG 5 cut(s) 313, 314, 549, 861, 1402
BstSFI CTRYAG 1 cut(s) 708
BstUI CGCG 3 cut(s) 416, 540, 1193
BstV1I GCAGC 5 cut(s) 146, 580, 602, 749, 1157
BstV2I GAAGAC 3 cut(s) 72, 1255, 1326
BstX2I RGATCY 5 cut(s) 686, 973, 1056, 1117, 1374
BstXI CCANNNNNNTGG 1 cut(s) 1394
BstYI RGATCY 5 cut(s) 686, 973, 1056, 1117, 1374
Bsu15I ATCGAT 2 cut(s) 681, 912
BsuRI GGCC 2 cut(s) 1050, 1236
BsuTUI ATCGAT 2 cut(s) 681, 912
BtrI CACGTC 1 cut(s) 248
BtsCI GGATG 4 cut(s) 100, 178, 202, 277
BtsIMutI CAGTG 3 cut(s) 453, 1227, 1305
Cac8I GCNNGC 7 cut(s) 286, 623, 822, 835, 896, 1175, 1365
CciI TCATGA 1 cut(s) 1356
CfoI GCGC 1 cut(s) 1193
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 4 cut(s) 25, 44, 242, 1407
Cfr9I CCCGGG 1 cut(s) 314
ClaI ATCGAT 2 cut(s) 681, 912
CpoI CGGWCCG 1 cut(s) 25
CsiI ACCWGGT 1 cut(s) 549
Csp6I GTAC 1 cut(s) 799
CspI CGGWCCG 1 cut(s) 25
CviAII CATG 8 cut(s) 297, 743, 821, 991, 1129, 1199, 1357, 1411
CviQI GTAC 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 708
EarI CTCTTC 1 cut(s) 708
Eco130I CCWWGG 2 cut(s) 215, 382
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 4 cut(s) 25, 44, 242, 1407
Eco88I CYCGRG 3 cut(s) 314, 482, 889
EcoNI CCTNNNNNAGG 1 cut(s) 1270
EcoRII CCWGG 2 cut(s) 549, 1402
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 2 cut(s) 215, 382
EcoT22I ATGCAT 2 cut(s) 822, 1156
ErhI CCWWGG 2 cut(s) 215, 382
FaeI CATG 8 cut(s) 300, 746, 824, 994, 1132, 1202, 1360, 1414
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 5 cut(s) 30, 297, 499, 807, 981
FatI CATG 8 cut(s) 296, 742, 820, 990, 1128, 1198, 1356, 1410
FauI CCCGC 4 cut(s) 55, 533, 887, 958
FauNDI CATATG 1 cut(s) 939
FbaI TGATCA 3 cut(s) 97, 330, 1302
Fnu4HI GCNGC 5 cut(s) 135, 591, 594, 738, 1171
FokI GGATG 4 cut(s) 87, 185, 189, 284
Fsp4HI GCNGC 5 cut(s) 135, 591, 594, 738, 1171
FspBI CTAG 2 cut(s) 216, 830
GlaI GCGC 1 cut(s) 1192
GluI GCNGC 5 cut(s) 135, 591, 594, 738, 1171
HaeIII GGCC 2 cut(s) 1050, 1236
HapII CCGG 5 cut(s) 28, 51, 315, 642, 863
HhaI GCGC 1 cut(s) 1193
Hin1II CATG 8 cut(s) 300, 746, 824, 994, 1132, 1202, 1360, 1414
Hin6I GCGC 1 cut(s) 1191
HinP1I GCGC 1 cut(s) 1191
HindIII AAGCTT 2 cut(s) 623, 757
HinfI GANTC 7 cut(s) 266, 378, 645, 807, 866, 998, 1068
HpaII CCGG 5 cut(s) 28, 51, 315, 642, 863
HphI GGTGA 3 cut(s) 955, 1312, 1409
Hpy166II GTNNAC 4 cut(s) 259, 847, 1002, 1400
Hpy188I TCNGA 7 cut(s) 25, 85, 271, 335, 985, 1146, 1159
Hpy8I GTNNAC 4 cut(s) 259, 847, 1002, 1400
Hpy99I CGWCG 3 cut(s) 89, 237, 1342
HpyAV CCTTC 3 cut(s) 878, 953, 1276
HpyCH4III ACNGT 7 cut(s) 181, 328, 448, 798, 851, 921, 1231
HpyCH4IV ACGT 3 cut(s) 69, 247, 724
HpyCH4V TGCA 9 cut(s) 134, 239, 288, 737, 815, 820, 956, 1154, 1173
HpyF10VI GCNNNNNNNGC 6 cut(s) 725, 734, 821, 830, 962, 1190
HpySE526I ACGT 3 cut(s) 69, 247, 724
Hsp92II CATG 8 cut(s) 300, 746, 824, 994, 1132, 1202, 1360, 1414
HspAI GCGC 1 cut(s) 1191
Ksp22I TGATCA 3 cut(s) 97, 330, 1302
LmnI GCTCC 1 cut(s) 587
Lsp1109I GCAGC 5 cut(s) 146, 580, 602, 749, 1157
LweI GCATC 2 cut(s) 1006, 1163
MabI ACCWGGT 1 cut(s) 549
MaeI CTAG 2 cut(s) 216, 830
MaeII ACGT 3 cut(s) 69, 247, 724
MaeIII GTNAC 5 cut(s) 37, 292, 398, 500, 1214
MboII GAAGA 7 cut(s) 43, 77, 725, 1028, 1102, 1260, 1326
MfeI CAATTG 1 cut(s) 657
MflI RGATCY 5 cut(s) 686, 973, 1056, 1117, 1374
MluCI AATT 3 cut(s) 657, 905, 951
MlyI GAGTC 2 cut(s) 275, 1007
MmeI TCCRAC 3 cut(s) 108, 249, 1145
Mph1103I ATGCAT 2 cut(s) 822, 1156
MroXI GAANNNNTTC 1 cut(s) 69
MseI TTAA 7 cut(s) 150, 321, 440, 546, 627, 788, 923
MslI CAYNNNNRTG 1 cut(s) 815
MspA1I CMGCKG 3 cut(s) 593, 707, 804
MspI CCGG 5 cut(s) 28, 51, 315, 642, 863
MspR9I CCNGG 5 cut(s) 315, 316, 551, 863, 1404
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 2 cut(s) 551, 1404
MvnI CGCG 3 cut(s) 416, 540, 1193
MwoI GCNNNNNNNGC 6 cut(s) 725, 734, 821, 830, 962, 1190
NciI CCSGG 3 cut(s) 315, 316, 863
NdeI CATATG 1 cut(s) 939
NlaIII CATG 8 cut(s) 300, 746, 824, 994, 1132, 1202, 1360, 1414
NlaIV GGNNCC 3 cut(s) 46, 183, 1408
NmeAIII GCCGAG 1 cut(s) 863
NmuCI GTSAC 1 cut(s) 500
NsiI ATGCAT 2 cut(s) 822, 1156
NspI RCATGY 3 cut(s) 300, 746, 824
PaeI GCATGC 1 cut(s) 824
PagI TCATGA 1 cut(s) 1356
PciI ACATGT 2 cut(s) 296, 742
PcsI WCGNNNNNNNCGW 1 cut(s) 1346
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 5 cut(s) 378, 645, 807, 866, 1068
PflFI GACNNNGTC 1 cut(s) 246
PflMI CCANNNNNTGG 1 cut(s) 1416
PfoI TCCNGGA 1 cut(s) 861
PkrI GCNGC 5 cut(s) 136, 592, 595, 739, 1172
PleI GAGTC 2 cut(s) 274, 1006
PpsI GAGTC 2 cut(s) 274, 1006
PscI ACATGT 2 cut(s) 296, 742
Psp6I CCWGG 2 cut(s) 549, 1402
PspGI CCWGG 2 cut(s) 549, 1402
PspN4I GGNNCC 3 cut(s) 46, 183, 1408
PspPI GGNCC 4 cut(s) 25, 44, 242, 1407
PsuI RGATCY 5 cut(s) 686, 973, 1056, 1117, 1374
PsyI GACNNNGTC 1 cut(s) 246
PvuII CAGCTG 2 cut(s) 593, 707
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 1 cut(s) 815
Rsr2I CGGWCCG 1 cut(s) 25
RsrII CGGWCCG 1 cut(s) 25
SaqAI TTAA 7 cut(s) 150, 321, 440, 546, 627, 788, 923
SatI GCNGC 5 cut(s) 135, 591, 594, 738, 1171
Sau96I GGNCC 4 cut(s) 25, 44, 242, 1407
SchI GAGTC 2 cut(s) 275, 1007
ScrFI CCNGG 5 cut(s) 315, 316, 551, 863, 1404
SexAI ACCWGGT 1 cut(s) 549
SfaNI GCATC 2 cut(s) 1006, 1163
SfcI CTRYAG 1 cut(s) 708
SinI GGWCC 4 cut(s) 25, 44, 242, 1407
SmaI CCCGGG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 815
SphI GCATGC 1 cut(s) 824
Sse9I AATT 3 cut(s) 657, 905, 951
SspMI CTAG 2 cut(s) 216, 830
StyD4I CCNGG 5 cut(s) 313, 314, 549, 861, 1402
StyI CCWWGG 2 cut(s) 215, 382
TaaI ACNGT 7 cut(s) 181, 328, 448, 798, 851, 921, 1231
TaiI ACGT 3 cut(s) 72, 250, 727
TaqI TCGA 6 cut(s) 165, 603, 681, 747, 912, 1337
TasI AATT 3 cut(s) 657, 905, 951
TfiI GAWTC 5 cut(s) 378, 645, 807, 866, 1068
Tru1I TTAA 7 cut(s) 150, 321, 440, 546, 627, 788, 923
Tru9I TTAA 7 cut(s) 150, 321, 440, 546, 627, 788, 923
TscAI CASTG 3 cut(s) 453, 1234, 1312
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 5 cut(s) 134, 590, 593, 737, 1170
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 6 cut(s) 191, 799, 1007, 1187, 1345, 1373
TspGWI ACGGA 2 cut(s) 221, 1295
TspMI CCCGGG 1 cut(s) 314
TspRI CASTG 3 cut(s) 453, 1234, 1312
Tth111I GACNNNGTC 1 cut(s) 246
Van91I CCANNNNNTGG 1 cut(s) 1416
VpaK11BI GGWCC 4 cut(s) 25, 44, 242, 1407
XagI CCTNNNNNAGG 1 cut(s) 1270
XapI RAATTY 1 cut(s) 905
XceI RCATGY 3 cut(s) 300, 746, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XmaI CCCGGG 1 cut(s) 314
XmaJI CCTAGG 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 2 cut(s) 216, 830
Zsp2I ATGCAT 2 cut(s) 822, 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.