Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000762

Physical Location & Seq

Genomic Coordinates Forward (+)

5591215 .. 5594532

Gene Structure 3318 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1068900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGCATTCAGTTTACAGTTCTTCCTCTGCAATCGTTTCACTGAAGGGTACAGGTATTTCGGATTTTTCGAACCCGGTTTCGGGTTCCAGAACTTGGTTGAAGAGTTCTTCGGGTTCGGGTCAGAAAGTCGGGTGCTTTTCTCGTTCCCAGATGGAGAACATGGGTAATAAGGTGGTGGTCGGCACCGGTGGTTATGTTCTCGAAGATGTTCCTCATTTCTCGGATTACATTCCTCATCTGCCGACCTACCCAAATCCGTTGCAAGACAATCCTGCATACTCAGTTGTCAAGCAGTATTTTGTTAATGTGGATGACACTGTTGCCCAAAAGATCGTTGTTCACAACAATAGTCCAAGGGGCACACATTTCCGGCGGGCAGGACCGCGTCAAAGAGTGTATTTCGAGTCGGATGATGTTCATGCCTGTATTGTGACATGTGGGGGTCTATGTCCTGGACTCAACACAGTGATTAGGGAGATAGTATGTGGCTTGCACCATATGTATGGTGTCAAGAGAGTTCTCGGAATTGATGGAGGATACCGGGGATTTTATTCACGTAATACGATTCCTTTGACTCCTAAGAGCGTGAACGACATCCATAAACGTGGTGGAACAATCCTTGGGACATCTCGTGGTGGCCACAATACCACAAAGATCGTTGACAGCATTCAAGATCGCGGAATCAATCAGGTTTACATAATTGGAGGAGATGGAACCCAAAAGGGTGCATCTGTGATATATGAGGAAATTAAGCGACGAGGTCTCAAAGTAGCAGTAGCCGGAATCCCCAAAACCATCGACAATGACATTCCGGTTATCGATAGGTCCTTTGGCTTTGACACTGCCGTTGAGGAGGCGCAACGTGCAATTAATGCAGCGCATGTCGAATCTGAAAGCATTGAGAATGGCATTGGTGTTGTAAAGCTGATGGGTCGCTACAGCGGGTTTATCGCTATGTATGCAACGCTTGCAAGCCGGGATGTAGACTGCTGCTTAATTCCTGAATCACCCTTTTACCTAGAAGGCCCGGGTGGACTTTTCGAGTACATAGAGAAACGACTCATAGAAAATGGGCACATGGTCATAGTCATGGCCGAAGGTGCAGGCCAGGAGCTTCTTTCTGAGAGCATTCATTCCATGACCAAACAGGACGCTTCAGGAAACAAGCTTCTCCAAGATGTTGGACTATGGATTTCCCAGAAGATCAAGGATCATTTTAAGGAGGGGAACAAAATGAGCATAAACCTCAAATATATAGATCCTACGTATATGATCCGAGCGGTTCCAAGCAATGCATCTGATAACGTGTACTGCACGCTCCTTGCTCAAAGCGCCGTCCATGGAGCAATGGCAGGTTATACAGGATTTACAGTTGGGCCTGTCAATGGGAGACATTCTTATATACCCTTCCATAGAATCATTGAGCAGCAGAACAAGGTTGTGATAACTGACAGAATGTGGGCGCGGCTCCTATCTTCGACAAACCAGCCGAGCTTCTTGAGTACCAAAGATGTCATCGAGGACAAGGCGGAAGAACCACCAACCCAGTTGCTGGATCACGAAAATGGTTTGGAGGTCAACTTGTTGAACAAGAAAATCAACTCTGCTTGTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230

Pfam Domains

Protein Families

PFK

Protein Analysis

539

Amino Acids

58.89

Weight (kDa)

7.28

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	142 - 447	2.1e-63	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26270 AT4G29220 AT4G32840 AT5G56630
fragaria_vesca	FvH4_1g06570 FvH4_2g35190 FvH4_6g32610 FvH4_6g32610 FvH4_6g32610
malus_domestica	MD02G1068900.v1.1 MD08G1109700.v1.1 MD09G1200000.v1.1 MD15G1089400.v1.1 MD17G1180600.v1.1 MD17G1180700.v1.1 MD17G1180800.v1.1
prunus_persica	Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.7G216700_v2.0.a1
pyrus_communis	pycom02g05460 pycom07g22220 pycom09g11720 pycom15g08380 pycom17g19000 pycom17g19010 pycom17g19050
rosa_chinensis	RchiOBHm_Chr2g0092311 RchiOBHm_Chr2g0141061 RchiOBHm_Chr6g0306751
rosa_laevigata	RLG00000010768 RLG00000016296 RLG00000019912
rosa_multiflora	Rmu_sc0003289.1_g000020 Rmu_sc0010596.1_g000001 Rmu_sc0025856.1_g000001 Rmu_sc0032226.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104240 Rroxscaffold_2G00149270 Rroxscaffold_4G00313720 Rroxscaffold_7G00161490
rosa_rugosa	Rorug02G0024700 Rorug02G0366600 Rorug02G0366800 Rorug04G0176400 Rorug06G0352400 Rorug06G0352500
rosa_samantha	Rh2AG417900 Rh2BG070100 Rh2BG427800 Rh2CG071300 Rh2CG404200 Rh2DG069100 Rh2DG437400 Rh2DG437800 Rh4CG359100 Rh6AG464500 Rh6BG436300 Rh6CG478800 Rh6DG019000 Rh6DG465900 Rh6DG478600 Rh7DG340800
rosa_wichuraiana	Rw2G006170 Rw2G034210 Rw2G034270 Rw6G040480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1343
AccB1I	GGYRCC	1 cut(s)	182
AccB7I	CCANNNNNTGG	2 cut(s)	93, 1552
AccBSI	CCGCTC	1 cut(s)	1280
AccI	GTMKAC	1 cut(s)	984
AccII	CGCG	3 cut(s)	385, 678, 1465
AciI	CCGC	7 cut(s)	373, 383, 678, 942, 1280, 1465, 1529
AclWI	GGATC	4 cut(s)	1218, 1253, 1267, 1563
AcoI	YGGCCR	2 cut(s)	637, 1092
AcuI	CTGAAG	2 cut(s)	62, 1140
AfaI	GTAC	4 cut(s)	49, 1046, 1310, 1504
AfiI	CCNNNNNNNGG	5 cut(s)	79, 80, 93, 1385, 1552
AflIII	ACRYGT	2 cut(s)	434, 1305
AgeI	ACCGGT	1 cut(s)	185
AgsI	TTSAA	3 cut(s)	100, 671, 1588
AjnI	CCWGG	2 cut(s)	451, 1107
AluBI	AGCT	4 cut(s)	925, 1114, 1168, 1494
AluI	AGCT	4 cut(s)	925, 1114, 1168, 1494
Alw26I	GTCTC	2 cut(s)	767, 1384
AlwI	GGATC	4 cut(s)	1218, 1253, 1267, 1563
AlwNI	CAGNNNCTG	1 cut(s)	1552
Ama87I	CYCGRG	1 cut(s)	1027
AoxI	GGCC	5 cut(s)	637, 1024, 1092, 1105, 1376
ApeKI	GCWGC	3 cut(s)	875, 990, 1426
AseI	ATTAAT	1 cut(s)	870
AsiGI	ACCGGT	1 cut(s)	185
Asp700I	GAANNNNTTC	1 cut(s)	207
AspLEI	GCGC	4 cut(s)	859, 880, 1334, 1465
AspS9I	GGNCC	4 cut(s)	380, 825, 1025, 1376
AsuC2I	CCSGG	5 cut(s)	74, 542, 977, 1028, 1029
AsuHPI	GGTGA	1 cut(s)	999
AsuII	TTCGAA	1 cut(s)	68
AvaI	CYCGRG	1 cut(s)	1027
AvaII	GGWCC	2 cut(s)	380, 825
BaeGI	GKGCMC	2 cut(s)	362, 1077
BalI	TGGCCA	1 cut(s)	639
BanI	GGYRCC	1 cut(s)	182
BauI	CACGAG	1 cut(s)	630
BbvI	GCAGC	3 cut(s)	887, 977, 1438
BccI	CCATC	5 cut(s)	145, 524, 704, 803, 922
BceAI	ACGGC	2 cut(s)	830, 1319
BciT130I	CCWGG	2 cut(s)	453, 1109
BciVI	GTATCC	1 cut(s)	530
BcnI	CCSGG	5 cut(s)	74, 542, 977, 1028, 1029
BcoDI	GTCTC	2 cut(s)	767, 1384
BfaI	CTAG	1 cut(s)	1019
BfmI	CTRYAG	1 cut(s)	937
BfoI	RGCGCY	1 cut(s)	1335
BfuAI	ACCTGC	1 cut(s)	1343
BfuI	GTATCC	1 cut(s)	530
BisI	GCNGC	4 cut(s)	876, 991, 1427, 1466
BlsI	GCNGC	4 cut(s)	877, 992, 1428, 1467
Bme1390I	CCNGG	7 cut(s)	74, 453, 542, 977, 1028, 1029, 1109
Bme18I	GGWCC	2 cut(s)	380, 825
BmeT110I	CYCGRG	1 cut(s)	1027
BmgT120I	GGNCC	4 cut(s)	380, 825, 1025, 1376
BmiI	GGNNCC	5 cut(s)	85, 184, 715, 1284, 1469
BmrFI	CCNGG	7 cut(s)	74, 453, 542, 977, 1028, 1029, 1109
BmrI	ACTGGG	1 cut(s)	1540
BmsI	GCATC	2 cut(s)	737, 1304
BmuI	ACTGGG	1 cut(s)	1540
Bpu14I	TTCGAA	1 cut(s)	68
BpuEI	CTTGAG	1 cut(s)	1519
BpuMI	CCSGG	5 cut(s)	74, 542, 977, 1028, 1029
Bsa29I	ATCGAT	1 cut(s)	819
BsaAI	YACGTR	2 cut(s)	557, 1266
BsaI	GGTCTC	1 cut(s)	767
BsaJI	CCNNGG	5 cut(s)	353, 541, 619, 1027, 1339
BsaWI	WCCGGW	2 cut(s)	185, 811
Bsc4I	CCNNNNNNNGG	5 cut(s)	79, 80, 93, 1385, 1552
Bse118I	RCCGGY	1 cut(s)	185
Bse1I	ACTGG	1 cut(s)	1546
Bse3DI	GCAATG	2 cut(s)	1297, 1353
BseBI	CCWGG	2 cut(s)	453, 1109
BseCI	ATCGAT	1 cut(s)	819
BseDI	CCNNGG	5 cut(s)	353, 541, 619, 1027, 1339
BseGI	GGATG	4 cut(s)	316, 415, 594, 985
BseLI	CCNNNNNNNGG	5 cut(s)	79, 80, 93, 1385, 1552
BseMI	GCAATG	2 cut(s)	1297, 1353
BseMII	CTCAG	2 cut(s)	294, 1113
BseNI	ACTGG	1 cut(s)	1546
BseRI	GAGGAG	2 cut(s)	720, 866
BseSI	GKGCMC	2 cut(s)	362, 1077
BseXI	GCAGC	3 cut(s)	887, 977, 1438
BsgI	GTGCAG	2 cut(s)	1122, 1297
Bsh1236I	CGCG	3 cut(s)	385, 678, 1465
BshFI	GGCC	5 cut(s)	639, 1026, 1094, 1107, 1378
BshNI	GGYRCC	1 cut(s)	182
BshTI	ACCGGT	1 cut(s)	185
BshVI	ATCGAT	1 cut(s)	819
BsiHKCI	CYCGRG	1 cut(s)	1027
BsiSI	CCGG	8 cut(s)	74, 186, 370, 541, 780, 812, 976, 1028
BslFI	GGGAC	1 cut(s)	637
BslI	CCNNNNNNNGG	5 cut(s)	79, 80, 93, 1385, 1552
BsmAI	GTCTC	2 cut(s)	767, 1384
BsmFI	GGGAC	1 cut(s)	637
BsmI	GAATGC	3 cut(s)	4, 666, 1128
BsnI	GGCC	5 cut(s)	639, 1026, 1094, 1107, 1378
Bso31I	GGTCTC	1 cut(s)	767
BsoBI	CYCGRG	1 cut(s)	1027
Bsp119I	TTCGAA	1 cut(s)	68
Bsp1286I	GDGCHC	2 cut(s)	362, 1077
Bsp143I	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
Bsp19I	CCATGG	1 cut(s)	1339
BspACI	CCGC	7 cut(s)	373, 383, 678, 942, 1280, 1465, 1529
BspANI	GGCC	5 cut(s)	639, 1026, 1094, 1107, 1378
BspCNI	CTCAG	2 cut(s)	293, 1114
BspDI	ATCGAT	1 cut(s)	819
BspFNI	CGCG	3 cut(s)	385, 678, 1465
BspLI	GGNNCC	5 cut(s)	85, 184, 715, 1284, 1469
BspMI	ACCTGC	1 cut(s)	1343
BspPI	GGATC	4 cut(s)	1218, 1253, 1267, 1563
BspT104I	TTCGAA	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	182
BspTNI	GGTCTC	1 cut(s)	767
BsrBI	CCGCTC	1 cut(s)	1280
BsrDI	GCAATG	2 cut(s)	1297, 1353
BsrFI	RCCGGY	1 cut(s)	185
BsrI	ACTGG	1 cut(s)	1546
BssAI	RCCGGY	1 cut(s)	185
BssECI	CCNNGG	5 cut(s)	353, 541, 619, 1027, 1339
BssMI	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
BssSI	CACGAG	1 cut(s)	630
BssT1I	CCWWGG	3 cut(s)	353, 619, 1339
Bst2BI	CACGAG	1 cut(s)	630
Bst2UI	CCWGG	2 cut(s)	453, 1109
Bst4CI	ACNGT	4 cut(s)	17, 319, 466, 1372
Bst6I	CTCTTC	1 cut(s)	95
BstAPI	GCANNNNNTGC	2 cut(s)	872, 968
BstBAI	YACGTR	2 cut(s)	557, 1266
BstBI	TTCGAA	1 cut(s)	68
BstC8I	GCNNGC	6 cut(s)	375, 491, 969, 973, 1105, 1316
BstDEI	CTNAG	3 cut(s)	280, 579, 1122
BstDSI	CCRYGG	1 cut(s)	1339
BstF5I	GGATG	4 cut(s)	316, 415, 594, 985
BstFNI	CGCG	3 cut(s)	385, 678, 1465
BstH2I	RGCGCY	1 cut(s)	1335
BstHHI	GCGC	4 cut(s)	859, 880, 1334, 1465
BstKTI	GATC	8 cut(s)	333, 657, 676, 1206, 1213, 1261, 1275, 1558
BstMAI	GTCTC	2 cut(s)	767, 1384
BstMBI	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
BstMWI	GCNNNNNNNGC	6 cut(s)	863, 872, 959, 968, 1100, 1331
BstNI	CCWGG	2 cut(s)	453, 1109
BstNSI	RCATGY	2 cut(s)	438, 884
BstSCI	CCNGG	7 cut(s)	72, 451, 540, 975, 1026, 1027, 1107
BstSFI	CTRYAG	1 cut(s)	937
BstSLI	GKGCMC	2 cut(s)	362, 1077
BstSNI	TACGTA	1 cut(s)	1266
BstUI	CGCG	3 cut(s)	385, 678, 1465
BstV1I	GCAGC	3 cut(s)	887, 977, 1438
BstX2I	RGATCY	1 cut(s)	1258
BstXI	CCANNNNNNTGG	3 cut(s)	503, 605, 1181
BstYI	RGATCY	1 cut(s)	1258
Bsu15I	ATCGAT	1 cut(s)	819
BsuI	GTATCC	1 cut(s)	530
BsuRI	GGCC	5 cut(s)	639, 1026, 1094, 1107, 1378
BsuTUI	ATCGAT	1 cut(s)	819
BtgI	CCRYGG	1 cut(s)	1339
BtsCI	GGATG	4 cut(s)	316, 415, 594, 985
BtsI	GCAGTG	1 cut(s)	840
BtsIMutI	CAGTG	4 cut(s)	38, 315, 471, 840
BveI	ACCTGC	1 cut(s)	1343
Cac8I	GCNNGC	6 cut(s)	375, 491, 969, 973, 1105, 1316
CaiI	CAGNNNCTG	1 cut(s)	1552
CfoI	GCGC	4 cut(s)	859, 880, 1334, 1465
Cfr10I	RCCGGY	1 cut(s)	185
Cfr13I	GGNCC	4 cut(s)	380, 825, 1025, 1376
Cfr9I	CCCGGG	1 cut(s)	1027
ClaI	ATCGAT	1 cut(s)	819
CseI	GACGC	2 cut(s)	374, 1160
Csp6I	GTAC	4 cut(s)	48, 1045, 1309, 1503
CspAI	ACCGGT	1 cut(s)	185
CviAII	CATG	8 cut(s)	160, 419, 435, 881, 1078, 1090, 1138, 1340
CviQI	GTAC	4 cut(s)	48, 1045, 1309, 1503
DdeI	CTNAG	3 cut(s)	280, 579, 1122
DpnI	GATC	8 cut(s)	332, 656, 675, 1205, 1212, 1260, 1274, 1557
DpnII	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
EaeI	YGGCCR	2 cut(s)	637, 1092
Eam1104I	CTCTTC	1 cut(s)	95
EarI	CTCTTC	1 cut(s)	95
EciI	GGCGGA	1 cut(s)	1544
Eco105I	TACGTA	1 cut(s)	1266
Eco130I	CCWWGG	3 cut(s)	353, 619, 1339
Eco31I	GGTCTC	1 cut(s)	767
Eco47I	GGWCC	2 cut(s)	380, 825
Eco57I	CTGAAG	2 cut(s)	62, 1140
Eco88I	CYCGRG	1 cut(s)	1027
EcoO109I	RGGNCCY	1 cut(s)	825
EcoRII	CCWGG	2 cut(s)	451, 1107
EcoT14I	CCWWGG	3 cut(s)	353, 619, 1339
EcoT22I	ATGCAT	2 cut(s)	6, 1297
ErhI	CCWWGG	3 cut(s)	353, 619, 1339
FaeI	CATG	8 cut(s)	163, 422, 438, 884, 1081, 1093, 1141, 1343
FaqI	GGGAC	1 cut(s)	637
FatI	CATG	8 cut(s)	159, 418, 434, 880, 1077, 1089, 1137, 1339
FauI	CCCGC	2 cut(s)	366, 935
FauNDI	CATATG	1 cut(s)	498
FblI	GTMKAC	1 cut(s)	984
Fnu4HI	GCNGC	4 cut(s)	876, 991, 1427, 1466
FokI	GGATG	4 cut(s)	323, 422, 581, 992
Fsp4HI	GCNGC	4 cut(s)	876, 991, 1427, 1466
FspBI	CTAG	1 cut(s)	1019
GlaI	GCGC	4 cut(s)	858, 879, 1333, 1464
GluI	GCNGC	4 cut(s)	876, 991, 1427, 1466
HaeII	RGCGCY	1 cut(s)	1335
HaeIII	GGCC	5 cut(s)	639, 1026, 1094, 1107, 1378
HapII	CCGG	8 cut(s)	74, 186, 370, 541, 780, 812, 976, 1028
HgaI	GACGC	2 cut(s)	374, 1160
HhaI	GCGC	4 cut(s)	859, 880, 1334, 1465
Hin1II	CATG	8 cut(s)	163, 422, 438, 884, 1081, 1093, 1141, 1343
Hin6I	GCGC	4 cut(s)	857, 878, 1332, 1463
HinP1I	GCGC	4 cut(s)	857, 878, 1332, 1463
HincII	GTYRAC	2 cut(s)	661, 1579
HindII	GTYRAC	2 cut(s)	661, 1579
HindIII	AAGCTT	1 cut(s)	1166
HpaII	CCGG	8 cut(s)	74, 186, 370, 541, 780, 812, 976, 1028
HphI	GGTGA	1 cut(s)	999
Hpy166II	GTNNAC	9 cut(s)	13, 340, 589, 661, 694, 985, 1034, 1309, 1579
Hpy188I	TCNGA	9 cut(s)	61, 123, 223, 409, 524, 892, 1123, 1277, 1300
Hpy188III	TCNNGA	8 cut(s)	87, 200, 511, 671, 1001, 1158, 1498, 1559
Hpy8I	GTNNAC	9 cut(s)	13, 340, 589, 661, 694, 985, 1034, 1309, 1579
Hpy99I	CGWCG	1 cut(s)	759
HpyAV	CCTTC	4 cut(s)	37, 1016, 1091, 1417
HpyCH4III	ACNGT	4 cut(s)	17, 319, 466, 1372
HpyCH4IV	ACGT	5 cut(s)	556, 604, 862, 1265, 1305
HpyF10VI	GCNNNNNNNGC	6 cut(s)	863, 872, 959, 968, 1100, 1331
HpyF3I	CTNAG	3 cut(s)	280, 579, 1122
HpySE526I	ACGT	5 cut(s)	556, 604, 862, 1265, 1305
Hsp92II	CATG	8 cut(s)	163, 422, 438, 884, 1081, 1093, 1141, 1343
HspAI	GCGC	4 cut(s)	857, 878, 1332, 1463
Kzo9I	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
LmnI	GCTCC	4 cut(s)	1111, 1323, 1343, 1473
Lsp1109I	GCAGC	3 cut(s)	887, 977, 1438
LweI	GCATC	2 cut(s)	737, 1304
MaeI	CTAG	1 cut(s)	1019
MaeII	ACGT	5 cut(s)	556, 604, 862, 1265, 1305
MaeIII	GTNAC	1 cut(s)	430
MalI	GATC	8 cut(s)	332, 656, 675, 1205, 1212, 1260, 1274, 1557
MbiI	CCGCTC	1 cut(s)	1280
MboI	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
MboII	GAAGA	7 cut(s)	12, 99, 112, 215, 1213, 1467, 1544
MflI	RGATCY	1 cut(s)	1258
MhlI	GDGCHC	2 cut(s)	362, 1077
MlsI	TGGCCA	1 cut(s)	639
MluCI	AATT	6 cut(s)	525, 699, 747, 867, 996, 1612
MluNI	TGGCCA	1 cut(s)	639
MlyI	GAGTC	4 cut(s)	413, 450, 568, 1053
MmeI	TCCRAC	2 cut(s)	387, 1162
Mox20I	TGGCCA	1 cut(s)	639
Mph1103I	ATGCAT	2 cut(s)	6, 1297
MroXI	GAANNNNTTC	1 cut(s)	207
MscI	TGGCCA	1 cut(s)	639
MseI	TTAA	5 cut(s)	303, 750, 870, 995, 1218
MslI	CAYNNNNRTG	4 cut(s)	501, 603, 1088, 1563
Msp20I	TGGCCA	1 cut(s)	639
MspA1I	CMGCKG	1 cut(s)	942
MspI	CCGG	8 cut(s)	74, 186, 370, 541, 780, 812, 976, 1028
MspR9I	CCNGG	7 cut(s)	74, 453, 542, 977, 1028, 1029, 1109
Mva1269I	GAATGC	3 cut(s)	4, 666, 1128
MvaI	CCWGG	2 cut(s)	453, 1109
MvnI	CGCG	3 cut(s)	385, 678, 1465
MwoI	GCNNNNNNNGC	6 cut(s)	863, 872, 959, 968, 1100, 1331
NciI	CCSGG	5 cut(s)	74, 542, 977, 1028, 1029
NcoI	CCATGG	1 cut(s)	1339
NdeI	CATATG	1 cut(s)	498
NdeII	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
NlaIII	CATG	8 cut(s)	163, 422, 438, 884, 1081, 1093, 1141, 1343
NlaIV	GGNNCC	5 cut(s)	85, 184, 715, 1284, 1469
NmeAIII	GCCGAG	1 cut(s)	1515
NmuCI	GTSAC	1 cut(s)	430
NsiI	ATGCAT	2 cut(s)	6, 1297
NspI	RCATGY	2 cut(s)	438, 884
NspV	TTCGAA	1 cut(s)	68
PciI	ACATGT	1 cut(s)	434
PcsI	WCGNNNNNNNCGW	1 cut(s)	65
PctI	GAATGC	3 cut(s)	4, 666, 1128
PdmI	GAANNNNTTC	1 cut(s)	207
PfeI	GAWTC	6 cut(s)	565, 681, 783, 887, 1004, 1416
PflFI	GACNNNGTC	2 cut(s)	384, 759
PflMI	CCANNNNNTGG	2 cut(s)	93, 1552
PfoI	TCCNGGA	1 cut(s)	451
PinAI	ACCGGT	1 cut(s)	185
PkrI	GCNGC	4 cut(s)	877, 992, 1428, 1467
PleI	GAGTC	4 cut(s)	412, 450, 568, 1053
PpsI	GAGTC	4 cut(s)	412, 450, 568, 1053
Ppu21I	YACGTR	2 cut(s)	557, 1266
PpuMI	RGGWCCY	1 cut(s)	825
PscI	ACATGT	1 cut(s)	434
PshBI	ATTAAT	1 cut(s)	870
Psp5II	RGGWCCY	1 cut(s)	825
Psp6I	CCWGG	2 cut(s)	451, 1107
PspGI	CCWGG	2 cut(s)	451, 1107
PspN4I	GGNNCC	5 cut(s)	85, 184, 715, 1284, 1469
PspPI	GGNCC	4 cut(s)	380, 825, 1025, 1376
PspPPI	RGGWCCY	1 cut(s)	825
PstNI	CAGNNNCTG	1 cut(s)	1552
PsuI	RGATCY	1 cut(s)	1258
PsyI	GACNNNGTC	2 cut(s)	384, 759
RsaI	GTAC	4 cut(s)	49, 1046, 1310, 1504
RsaNI	GTAC	4 cut(s)	48, 1045, 1309, 1503
RseI	CAYNNNNRTG	4 cut(s)	501, 603, 1088, 1563
SaqAI	TTAA	5 cut(s)	303, 750, 870, 995, 1218
SatI	GCNGC	4 cut(s)	876, 991, 1427, 1466
Sau3AI	GATC	8 cut(s)	330, 654, 673, 1203, 1210, 1258, 1272, 1555
Sau96I	GGNCC	4 cut(s)	380, 825, 1025, 1376
SchI	GAGTC	4 cut(s)	413, 450, 568, 1053
ScrFI	CCNGG	7 cut(s)	74, 453, 542, 977, 1028, 1029, 1109
SduI	GDGCHC	2 cut(s)	362, 1077
SfaNI	GCATC	2 cut(s)	737, 1304
SfcI	CTRYAG	1 cut(s)	937
SfuI	TTCGAA	1 cut(s)	68
SgrAI	CRCCGGYG	1 cut(s)	185
SinI	GGWCC	2 cut(s)	380, 825
SmaI	CCCGGG	1 cut(s)	1029
SmiMI	CAYNNNNRTG	4 cut(s)	501, 603, 1088, 1563
SmlI	CTYRAG	1 cut(s)	1498
SmoI	CTYRAG	1 cut(s)	1498
SnaBI	TACGTA	1 cut(s)	1266
Sse9I	AATT	6 cut(s)	525, 699, 747, 867, 996, 1612
SsiI	CCGC	7 cut(s)	373, 383, 678, 942, 1280, 1465, 1529
SspMI	CTAG	1 cut(s)	1019
StyD4I	CCNGG	7 cut(s)	72, 451, 540, 975, 1026, 1027, 1107
StyI	CCWWGG	3 cut(s)	353, 619, 1339
TaaI	ACNGT	4 cut(s)	17, 319, 466, 1372
TaiI	ACGT	5 cut(s)	559, 607, 865, 1268, 1308
TaqI	TCGA	9 cut(s)	68, 201, 402, 798, 819, 885, 1041, 1478, 1518
TasI	AATT	6 cut(s)	525, 699, 747, 867, 996, 1612
TatI	WGTACW	2 cut(s)	1044, 1308
TauI	GCSGC	1 cut(s)	1468
TfiI	GAWTC	6 cut(s)	565, 681, 783, 887, 1004, 1416
Tru1I	TTAA	5 cut(s)	303, 750, 870, 995, 1218
Tru9I	TTAA	5 cut(s)	303, 750, 870, 995, 1218
TscAI	CASTG	4 cut(s)	45, 322, 471, 847
TseFI	GTSAC	1 cut(s)	430
TseI	GCWGC	3 cut(s)	875, 990, 1426
Tsp45I	GTSAC	1 cut(s)	430
TspDTI	ATGAA	2 cut(s)	407, 1121
TspGWI	ACGGA	1 cut(s)	246
TspMI	CCCGGG	1 cut(s)	1027
TspRI	CASTG	4 cut(s)	45, 322, 471, 847
Tth111I	GACNNNGTC	2 cut(s)	384, 759
Van91I	CCANNNNNTGG	2 cut(s)	93, 1552
VpaK11BI	GGWCC	2 cut(s)	380, 825
VspI	ATTAAT	1 cut(s)	870
XceI	RCATGY	2 cut(s)	438, 884
XcmI	CCANNNNNNNNNTGG	1 cut(s)	605
XmaI	CCCGGG	1 cut(s)	1027
XmiI	GTMKAC	1 cut(s)	984
XmnI	GAANNNNTTC	1 cut(s)	207
XspI	CTAG	1 cut(s)	1019
Zsp2I	ATGCAT	2 cut(s)	6, 1297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.