Rh6DG019000

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000762
Physical Location & Seq
Forward (+)
1727295 .. 1735403
8109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG019000.1

Sequence Viewer

Length: 717 bp
ATGGGAACGATGAGTGCGTTTCAAAAAATTCATACAAGAGACTCTTTTTTCTACGATGCCTGGCAGAACCCGCAACATCGTGCGAGTCTCCTTTCAACACAAGAACAGATTAGCTTTTCACTTCTATCTTTTGATGCACGGTTTCCCGATATGTGTAACCAACTAACACAAACAAAAGTCTTGAGCATCAAAGATCAGGGGGATGTTCCTACGAGATATATTTATCTTACTGCTGAGATTCTTAAAGAAGTGTTCAAGGTTCTGAGAAATGTTCAAATGCAGGACTCTGACATATTTTCGGGATTCTCGATACGACTGATAGTTTATAAGAGCTTCATCTGGGATTCCTCGGTATTTGCATCTGGAAGCCTCTATCAGATAGAGACTATCTACACTTCTACCTTTATCAATTATCAACAACGATCGAGAGAGAGAGAGATAAGAAATCGGTTGTGTTTCTTTGCTCTGCACGAAGAGATAGAGAGAGATCGGAGGCAAGTTCTCACCTCATCATCTGAGATCCGAGGGAGGATACATAGGGTTTTTGCCAGAAGTACAATTCCCTTAACACCAAAGATTGTGAATGACATCCATAAACGTGGTGACACCAACCTTGGGACATCACGAGGTAGCCATGATACCTCAAAGATTACGGACAGCATTCAGAACAGAGGAATTAAGATTTTAATGGCTACATTTGTTTTCCCATACTCTTGA

Protein Analysis

238

Amino Acids

27.71

Weight (kDa)

9.51

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AciI CCGC 1 cut(s) 71
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 1 cut(s) 27
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 1 cut(s) 615
AgsI TTSAA 4 cut(s) 23, 96, 256, 275
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 2 cut(s) 114, 333
AluI AGCT 2 cut(s) 114, 333
Alw26I GTCTC 3 cut(s) 33, 92, 377
AlwI GGATC 1 cut(s) 514
ApoI RAATTY 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 496, 614
BauI CACGAG 1 cut(s) 624
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 1 cut(s) 525
BcoDI GTCTC 3 cut(s) 33, 92, 377
BfuI GTATCC 1 cut(s) 525
Bme1390I CCNGG 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 4 cut(s) 46, 124, 195, 368
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 3 cut(s) 348, 523, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 615
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 348, 523, 613
BseGI GGATG 2 cut(s) 208, 588
BseLI CCNNNNNNNGG 1 cut(s) 615
BseMII CTCAG 3 cut(s) 225, 254, 507
BsgI GTGCAG 1 cut(s) 452
Bsh1285I CGRYCG 1 cut(s) 425
BsiEI CGRYCG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 631
BslI CCNNNNNNNGG 1 cut(s) 615
BsmAI GTCTC 3 cut(s) 33, 92, 377
BsmFI GGGAC 1 cut(s) 631
BsmI GAATGC 1 cut(s) 660
Bsp143I GATC 4 cut(s) 193, 422, 487, 519
BspACI CCGC 1 cut(s) 71
BspCNI CTCAG 3 cut(s) 226, 255, 508
BspPI GGATC 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 348, 523, 613
BssMI GATC 4 cut(s) 193, 422, 487, 519
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 1 cut(s) 613
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 468
BstDEI CTNAG 3 cut(s) 234, 263, 516
BstF5I GGATG 2 cut(s) 208, 588
BstKTI GATC 4 cut(s) 196, 425, 490, 522
BstMAI GTCTC 3 cut(s) 33, 92, 377
BstMBI GATC 4 cut(s) 193, 422, 487, 519
BstMCI CGRYCG 1 cut(s) 425
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 1 cut(s) 519
BsuI GTATCC 1 cut(s) 525
BtsCI GGATG 2 cut(s) 208, 588
Csp6I GTAC 1 cut(s) 555
CviAII CATG 1 cut(s) 635
CviJI RGCY 5 cut(s) 114, 333, 369, 633, 692
CviKI_1 RGCY 5 cut(s) 114, 333, 369, 633, 692
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 3 cut(s) 234, 263, 516
DpnI GATC 4 cut(s) 195, 424, 489, 521
DpnII GATC 4 cut(s) 193, 422, 487, 519
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 613
FaeI CATG 1 cut(s) 638
FaiI YATR 9 cut(s) 33, 152, 219, 293, 327, 537, 594, 636, 709
FalI AAGNNNNNCTT 2 cut(s) 28, 60
FaqI GGGAC 1 cut(s) 631
FatI CATG 1 cut(s) 634
FauI CCCGC 1 cut(s) 78
FokI GGATG 2 cut(s) 215, 575
Hin1II CATG 1 cut(s) 638
HinfI GANTC 6 cut(s) 41, 85, 238, 284, 303, 344
HphI GGTGA 2 cut(s) 496, 614
Hpy188I TCNGA 7 cut(s) 264, 289, 378, 492, 517, 524, 666
Hpy188III TCNNGA 8 cut(s) 146, 181, 300, 307, 363, 426, 624, 714
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 4 cut(s) 137, 280, 359, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 3 cut(s) 234, 263, 516
HpySE526I ACGT 1 cut(s) 598
Hsp92II CATG 1 cut(s) 638
Kzo9I GATC 4 cut(s) 193, 422, 487, 519
LpnPI CCDG 7 cut(s) 46, 73, 182, 266, 325, 348, 562
LweI GCATC 4 cut(s) 46, 124, 195, 368
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 2 cut(s) 155, 602
MalI GATC 4 cut(s) 195, 424, 489, 521
MboI GATC 4 cut(s) 193, 422, 487, 519
MboII GAAGA 1 cut(s) 485
MflI RGATCY 1 cut(s) 519
MluCI AATT 4 cut(s) 27, 409, 558, 675
MlyI GAGTC 3 cut(s) 35, 94, 278
MnlI CCTC 9 cut(s) 358, 380, 486, 517, 518, 522, 620, 652, 665
MseI TTAA 4 cut(s) 243, 566, 678, 686
MslI CAYNNNNRTG 1 cut(s) 597
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 660
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 4 cut(s) 193, 422, 487, 519
NlaIII CATG 1 cut(s) 638
NmuCI GTSAC 1 cut(s) 602
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 305
PctI GAATGC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 238, 303, 344
Ple19I CGATCG 1 cut(s) 425
PleI GAGTC 3 cut(s) 35, 93, 278
PpsI GAGTC 3 cut(s) 35, 93, 278
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PsuI RGATCY 1 cut(s) 519
PvuI CGATCG 1 cut(s) 425
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 597
SaqAI TTAA 4 cut(s) 243, 566, 678, 686
Sau3AI GATC 4 cut(s) 193, 422, 487, 519
SchI GAGTC 3 cut(s) 35, 94, 278
ScrFI CCNGG 1 cut(s) 61
SetI ASST 9 cut(s) 116, 261, 335, 404, 509, 601, 615, 631, 644
SfaNI GCATC 4 cut(s) 46, 124, 195, 368
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 4 cut(s) 27, 409, 558, 675
SsiI CCGC 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 1 cut(s) 141
TaiI ACGT 1 cut(s) 601
TaqI TCGA 2 cut(s) 308, 425
TasI AATT 4 cut(s) 27, 409, 558, 675
TatI WGTACW 1 cut(s) 554
TfiI GAWTC 3 cut(s) 238, 303, 344
Tru1I TTAA 4 cut(s) 243, 566, 678, 686
Tru9I TTAA 4 cut(s) 243, 566, 678, 686
TseFI GTSAC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 2 cut(s) 20, 325
TspGWI ACGGA 1 cut(s) 668
XapI RAATTY 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.