Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 141 - 447 | 2.4e-62 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1019 |
| AatII | GACGTC | 1 cut(s) | 210 |
| Acc36I | ACCTGC | 1 cut(s) | 1019 |
| AccB1I | GGYRCC | 1 cut(s) | 611 |
| AccI | GTMKAC | 1 cut(s) | 1308 |
| AccII | CGCG | 1 cut(s) | 385 |
| AciI | CCGC | 6 cut(s) | 22, 70, 73, 153, 383, 534 |
| AclWI | GGATC | 4 cut(s) | 1253, 1267, 1496, 1509 |
| AcoI | YGGCCR | 3 cut(s) | 3, 637, 1072 |
| AcsI | RAATTY | 3 cut(s) | 147, 516, 783 |
| AcuI | CTGAAG | 2 cut(s) | 61, 1116 |
| AcyI | GRCGYC | 2 cut(s) | 56, 207 |
| AdeI | CACNNNGTG | 1 cut(s) | 635 |
| AfaI | GTAC | 4 cut(s) | 107, 494, 715, 1046 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 15, 621, 982, 1573 |
| AflIII | ACRYGT | 2 cut(s) | 434, 498 |
| AgsI | TTSAA | 5 cut(s) | 96, 404, 887, 1052, 1175 |
| AjiI | CACGTC | 1 cut(s) | 374 |
| AjnI | CCWGG | 3 cut(s) | 101, 451, 1548 |
| AluBI | AGCT | 8 cut(s) | 728, 878, 953, 1094, 1114, 1121, 1168, 1517 |
| AluI | AGCT | 8 cut(s) | 728, 878, 953, 1094, 1114, 1121, 1168, 1517 |
| Alw26I | GTCTC | 2 cut(s) | 702, 1420 |
| AlwI | GGATC | 4 cut(s) | 1253, 1267, 1496, 1509 |
| AoxI | GGCC | 4 cut(s) | 3, 637, 760, 1072 |
| ApeKI | GCWGC | 3 cut(s) | 24, 50, 875 |
| ApoI | RAATTY | 3 cut(s) | 147, 516, 783 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 812, 844 |
| AseI | ATTAAT | 2 cut(s) | 684, 870 |
| AspLEI | GCGC | 1 cut(s) | 1103 |
| AspS9I | GGNCC | 2 cut(s) | 380, 1210 |
| AsuHPI | GGTGA | 4 cut(s) | 56, 169, 1360, 1429 |
| AvaII | GGWCC | 2 cut(s) | 380, 1210 |
| BaeGI | GKGCMC | 2 cut(s) | 140, 1465 |
| BalI | TGGCCA | 2 cut(s) | 5, 639 |
| BamHI | GGATCC | 1 cut(s) | 1501 |
| BanI | GGYRCC | 1 cut(s) | 611 |
| BanII | GRGCYC | 1 cut(s) | 168 |
| BauI | CACGAG | 1 cut(s) | 630 |
| BbsI | GAAGAC | 1 cut(s) | 210 |
| BbvI | GCAGC | 3 cut(s) | 11, 37, 887 |
| BccI | CCATC | 2 cut(s) | 623, 1419 |
| BceAI | ACGGC | 2 cut(s) | 1087, 1481 |
| BciT130I | CCWGG | 3 cut(s) | 103, 453, 1550 |
| BciVI | GTATCC | 2 cut(s) | 530, 1349 |
| BcoDI | GTCTC | 2 cut(s) | 702, 1420 |
| BfaI | CTAG | 1 cut(s) | 1118 |
| BfmI | CTRYAG | 1 cut(s) | 937 |
| BfuAI | ACCTGC | 1 cut(s) | 1019 |
| BfuI | GTATCC | 2 cut(s) | 530, 1349 |
| BisI | GCNGC | 4 cut(s) | 25, 51, 73, 876 |
| BlsI | GCNGC | 4 cut(s) | 26, 52, 74, 877 |
| Bme1390I | CCNGG | 3 cut(s) | 103, 453, 1550 |
| Bme18I | GGWCC | 2 cut(s) | 380, 1210 |
| BmgBI | CACGTC | 1 cut(s) | 374 |
| BmgT120I | GGNCC | 2 cut(s) | 380, 1210 |
| BmiI | GGNNCC | 3 cut(s) | 20, 613, 1503 |
| BmrFI | CCNGG | 3 cut(s) | 103, 453, 1550 |
| BmsI | GCATC | 1 cut(s) | 1304 |
| BpiI | GAAGAC | 1 cut(s) | 210 |
| BsaHI | GRCGYC | 2 cut(s) | 56, 207 |
| BsaJI | CCNNGG | 4 cut(s) | 14, 101, 132, 619 |
| BsaWI | WCCGGW | 1 cut(s) | 811 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 113, 143, 717, 747 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 15, 621, 982, 1573 |
| Bse118I | RCCGGY | 2 cut(s) | 179, 1361 |
| Bse3DI | GCAATG | 2 cut(s) | 1297, 1353 |
| BseBI | CCWGG | 3 cut(s) | 103, 453, 1550 |
| BseDI | CCNNGG | 4 cut(s) | 14, 101, 132, 619 |
| BseGI | GGATG | 3 cut(s) | 228, 594, 615 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 15, 621, 982, 1573 |
| BseMI | GCAATG | 2 cut(s) | 1297, 1353 |
| BseRI | GAGGAG | 1 cut(s) | 866 |
| BseSI | GKGCMC | 2 cut(s) | 140, 1465 |
| BseXI | GCAGC | 3 cut(s) | 11, 37, 887 |
| BsgI | GTGCAG | 1 cut(s) | 1297 |
| Bsh1236I | CGCG | 1 cut(s) | 385 |
| BshFI | GGCC | 4 cut(s) | 5, 639, 762, 1074 |
| BshNI | GGYRCC | 1 cut(s) | 611 |
| BsiSI | CCGG | 3 cut(s) | 180, 812, 1362 |
| BslFI | GGGAC | 2 cut(s) | 194, 637 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 15, 621, 982, 1573 |
| BsmAI | GTCTC | 2 cut(s) | 702, 1420 |
| BsmBI | CGTCTC | 1 cut(s) | 702 |
| BsmFI | GGGAC | 2 cut(s) | 194, 637 |
| BsmI | GAATGC | 2 cut(s) | 666, 752 |
| BsnI | GGCC | 4 cut(s) | 5, 639, 762, 1074 |
| Bsp1286I | GDGCHC | 3 cut(s) | 140, 168, 1465 |
| Bsp143I | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| BspACI | CCGC | 6 cut(s) | 22, 70, 73, 153, 383, 534 |
| BspANI | GGCC | 4 cut(s) | 5, 639, 762, 1074 |
| BspFNI | CGCG | 1 cut(s) | 385 |
| BspLI | GGNNCC | 3 cut(s) | 20, 613, 1503 |
| BspMI | ACCTGC | 1 cut(s) | 1019 |
| BspPI | GGATC | 4 cut(s) | 1253, 1267, 1496, 1509 |
| BspT107I | GGYRCC | 1 cut(s) | 611 |
| BsrDI | GCAATG | 2 cut(s) | 1297, 1353 |
| BsrFI | RCCGGY | 2 cut(s) | 179, 1361 |
| BssAI | RCCGGY | 2 cut(s) | 179, 1361 |
| BssECI | CCNNGG | 4 cut(s) | 14, 101, 132, 619 |
| BssMI | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| BssNAI | GTATAC | 1 cut(s) | 1309 |
| BssNI | GRCGYC | 2 cut(s) | 56, 207 |
| BssSI | CACGAG | 1 cut(s) | 630 |
| BssT1I | CCWWGG | 2 cut(s) | 132, 619 |
| Bst1107I | GTATAC | 1 cut(s) | 1309 |
| Bst2BI | CACGAG | 1 cut(s) | 630 |
| Bst2UI | CCWGG | 3 cut(s) | 103, 453, 1550 |
| Bst4CI | ACNGT | 5 cut(s) | 18, 466, 713, 941, 1372 |
| BstACI | GRCGYC | 2 cut(s) | 56, 207 |
| BstC8I | GCNNGC | 5 cut(s) | 190, 973, 1130, 1134, 1395 |
| BstDSI | CCRYGG | 1 cut(s) | 14 |
| BstF5I | GGATG | 3 cut(s) | 228, 594, 615 |
| BstFNI | CGCG | 1 cut(s) | 385 |
| BstHHI | GCGC | 1 cut(s) | 1103 |
| BstKTI | GATC | 6 cut(s) | 64, 174, 238, 1261, 1275, 1504 |
| BstMAI | GTCTC | 2 cut(s) | 702, 1420 |
| BstMBI | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 78, 189, 863, 872, 959, 968, 1100 |
| BstNI | CCWGG | 3 cut(s) | 103, 453, 1550 |
| BstNSI | RCATGY | 4 cut(s) | 426, 438, 502, 1397 |
| BstSCI | CCNGG | 3 cut(s) | 101, 451, 1548 |
| BstSFI | CTRYAG | 1 cut(s) | 937 |
| BstSLI | GKGCMC | 2 cut(s) | 140, 1465 |
| BstUI | CGCG | 1 cut(s) | 385 |
| BstV1I | GCAGC | 3 cut(s) | 11, 37, 887 |
| BstV2I | GAAGAC | 1 cut(s) | 210 |
| BstX2I | RGATCY | 2 cut(s) | 1258, 1501 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 605 |
| BstYI | RGATCY | 2 cut(s) | 1258, 1501 |
| BstZ17I | GTATAC | 1 cut(s) | 1309 |
| BsuI | GTATCC | 2 cut(s) | 530, 1349 |
| BsuRI | GGCC | 4 cut(s) | 5, 639, 762, 1074 |
| BtgI | CCRYGG | 1 cut(s) | 14 |
| BtrI | CACGTC | 1 cut(s) | 374 |
| BtsCI | GGATG | 3 cut(s) | 228, 594, 615 |
| BtsI | GCAGTG | 2 cut(s) | 780, 840 |
| BtsIMutI | CAGTG | 3 cut(s) | 780, 840, 946 |
| BveI | ACCTGC | 1 cut(s) | 1019 |
| Cac8I | GCNNGC | 5 cut(s) | 190, 973, 1130, 1134, 1395 |
| CfoI | GCGC | 1 cut(s) | 1103 |
| Cfr10I | RCCGGY | 2 cut(s) | 179, 1361 |
| Cfr13I | GGNCC | 2 cut(s) | 380, 1210 |
| CseI | GACGC | 4 cut(s) | 64, 194, 374, 1160 |
| CsiI | ACCWGGT | 1 cut(s) | 1548 |
| Csp6I | GTAC | 4 cut(s) | 106, 493, 714, 1045 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 639, 674 |
| CviAII | CATG | 9 cut(s) | 423, 435, 499, 641, 881, 1078, 1270, 1340, 1394 |
| CviQI | GTAC | 4 cut(s) | 106, 493, 714, 1045 |
| DpnI | GATC | 6 cut(s) | 63, 173, 237, 1260, 1274, 1503 |
| DpnII | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| DraIII | CACNNNGTG | 1 cut(s) | 635 |
| EaeI | YGGCCR | 3 cut(s) | 3, 637, 1072 |
| EciI | GGCGGA | 2 cut(s) | 142, 549 |
| Eco130I | CCWWGG | 2 cut(s) | 132, 619 |
| Eco24I | GRGCYC | 1 cut(s) | 168 |
| Eco47I | GGWCC | 2 cut(s) | 380, 1210 |
| Eco57I | CTGAAG | 2 cut(s) | 61, 1116 |
| EcoRI | GAATTC | 2 cut(s) | 516, 783 |
| EcoRII | CCWGG | 3 cut(s) | 101, 451, 1548 |
| EcoT14I | CCWWGG | 2 cut(s) | 132, 619 |
| EcoT22I | ATGCAT | 1 cut(s) | 1297 |
| EcoT38I | GRGCYC | 1 cut(s) | 168 |
| ErhI | CCWWGG | 2 cut(s) | 132, 619 |
| Esp3I | CGTCTC | 1 cut(s) | 702 |
| FaeI | CATG | 9 cut(s) | 426, 438, 502, 644, 884, 1081, 1273, 1343, 1397 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1199, 1231, 1381, 1413 |
| FaqI | GGGAC | 2 cut(s) | 194, 637 |
| FatI | CATG | 9 cut(s) | 422, 434, 498, 640, 880, 1077, 1269, 1339, 1393 |
| FauI | CCCGC | 1 cut(s) | 77 |
| FblI | GTMKAC | 1 cut(s) | 1308 |
| Fnu4HI | GCNGC | 4 cut(s) | 25, 51, 73, 876 |
| FokI | GGATG | 3 cut(s) | 215, 581, 602 |
| FriOI | GRGCYC | 1 cut(s) | 168 |
| Fsp4HI | GCNGC | 4 cut(s) | 25, 51, 73, 876 |
| FspBI | CTAG | 1 cut(s) | 1118 |
| GlaI | GCGC | 1 cut(s) | 1102 |
| GluI | GCNGC | 4 cut(s) | 25, 51, 73, 876 |
| HaeIII | GGCC | 4 cut(s) | 5, 639, 762, 1074 |
| HapII | CCGG | 3 cut(s) | 180, 812, 1362 |
| HgaI | GACGC | 4 cut(s) | 64, 194, 374, 1160 |
| HhaI | GCGC | 1 cut(s) | 1103 |
| Hin1I | GRCGYC | 2 cut(s) | 56, 207 |
| Hin1II | CATG | 9 cut(s) | 426, 438, 502, 644, 884, 1081, 1273, 1343, 1397 |
| Hin6I | GCGC | 1 cut(s) | 1101 |
| HinP1I | GCGC | 1 cut(s) | 1101 |
| HindIII | AAGCTT | 1 cut(s) | 1166 |
| HinfI | GANTC | 4 cut(s) | 997, 1004, 1141, 1178 |
| HpaII | CCGG | 3 cut(s) | 180, 812, 1362 |
| HphI | GGTGA | 4 cut(s) | 56, 169, 1360, 1429 |
| Hpy166II | GTNNAC | 5 cut(s) | 511, 661, 694, 716, 1309 |
| Hpy188I | TCNGA | 5 cut(s) | 163, 223, 390, 1300, 1506 |
| Hpy8I | GTNNAC | 5 cut(s) | 511, 661, 694, 716, 1309 |
| Hpy99I | CGWCG | 4 cut(s) | 58, 188, 375, 1376 |
| HpyAV | CCTTC | 6 cut(s) | 282, 385, 1016, 1091, 1383, 1501 |
| HpyCH4III | ACNGT | 5 cut(s) | 18, 466, 713, 941, 1372 |
| HpyCH4IV | ACGT | 4 cut(s) | 195, 207, 373, 604 |
| HpyCH4V | TGCA | 7 cut(s) | 422, 750, 773, 875, 1295, 1314, 1346 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 78, 189, 863, 872, 959, 968, 1100 |
| HpySE526I | ACGT | 4 cut(s) | 195, 207, 373, 604 |
| Hsp92I | GRCGYC | 2 cut(s) | 56, 207 |
| Hsp92II | CATG | 9 cut(s) | 426, 438, 502, 644, 884, 1081, 1273, 1343, 1397 |
| HspAI | GCGC | 1 cut(s) | 1101 |
| Kzo9I | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| LmnI | GCTCC | 2 cut(s) | 725, 1025 |
| Lsp1109I | GCAGC | 3 cut(s) | 11, 37, 887 |
| LweI | GCATC | 1 cut(s) | 1304 |
| MabI | ACCWGGT | 1 cut(s) | 1548 |
| MaeI | CTAG | 1 cut(s) | 1118 |
| MaeII | ACGT | 4 cut(s) | 195, 207, 373, 604 |
| MaeIII | GTNAC | 2 cut(s) | 430, 1417 |
| MalI | GATC | 6 cut(s) | 63, 173, 237, 1260, 1274, 1503 |
| MboI | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| MboII | GAAGA | 4 cut(s) | 215, 1163, 1213, 1544 |
| MflI | RGATCY | 2 cut(s) | 1258, 1501 |
| MhlI | GDGCHC | 3 cut(s) | 140, 168, 1465 |
| MlsI | TGGCCA | 2 cut(s) | 5, 639 |
| MluCI | AATT | 8 cut(s) | 91, 147, 468, 516, 564, 681, 783, 1633 |
| MluNI | TGGCCA | 2 cut(s) | 5, 639 |
| MlyI | GAGTC | 1 cut(s) | 1013 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 639 |
| Mph1103I | ATGCAT | 1 cut(s) | 1297 |
| MscI | TGGCCA | 2 cut(s) | 5, 639 |
| MslI | CAYNNNNRTG | 1 cut(s) | 603 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 639 |
| MspA1I | CMGCKG | 1 cut(s) | 24 |
| MspI | CCGG | 3 cut(s) | 180, 812, 1362 |
| MspR9I | CCNGG | 3 cut(s) | 103, 453, 1550 |
| Mva1269I | GAATGC | 2 cut(s) | 666, 752 |
| MvaI | CCWGG | 3 cut(s) | 103, 453, 1550 |
| MvnI | CGCG | 1 cut(s) | 385 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 78, 189, 863, 872, 959, 968, 1100 |
| NdeII | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| NlaIII | CATG | 9 cut(s) | 426, 438, 502, 644, 884, 1081, 1273, 1343, 1397 |
| NlaIV | GGNNCC | 3 cut(s) | 20, 613, 1503 |
| NmeAIII | GCCGAG | 1 cut(s) | 1001 |
| NmuCI | GTSAC | 2 cut(s) | 430, 1417 |
| NsiI | ATGCAT | 1 cut(s) | 1297 |
| NspI | RCATGY | 4 cut(s) | 426, 438, 502, 1397 |
| PaeI | GCATGC | 1 cut(s) | 1397 |
| PaqCI | CACCTGC | 1 cut(s) | 1019 |
| PciI | ACATGT | 2 cut(s) | 434, 498 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 192, 306 |
| PctI | GAATGC | 2 cut(s) | 666, 752 |
| PfeI | GAWTC | 3 cut(s) | 997, 1141, 1178 |
| PflFI | GACNNNGTC | 1 cut(s) | 384 |
| PkrI | GCNGC | 4 cut(s) | 26, 52, 74, 877 |
| PleI | GAGTC | 1 cut(s) | 1012 |
| PpsI | GAGTC | 1 cut(s) | 1012 |
| PscI | ACATGT | 2 cut(s) | 434, 498 |
| PshBI | ATTAAT | 2 cut(s) | 684, 870 |
| Psp6I | CCWGG | 3 cut(s) | 101, 451, 1548 |
| PspGI | CCWGG | 3 cut(s) | 101, 451, 1548 |
| PspN4I | GGNNCC | 3 cut(s) | 20, 613, 1503 |
| PspPI | GGNCC | 2 cut(s) | 380, 1210 |
| PsuI | RGATCY | 2 cut(s) | 1258, 1501 |
| PsyI | GACNNNGTC | 1 cut(s) | 384 |
| RsaI | GTAC | 4 cut(s) | 107, 494, 715, 1046 |
| RsaNI | GTAC | 4 cut(s) | 106, 493, 714, 1045 |
| RseI | CAYNNNNRTG | 1 cut(s) | 603 |
| SatI | GCNGC | 4 cut(s) | 25, 51, 73, 876 |
| Sau3AI | GATC | 6 cut(s) | 61, 171, 235, 1258, 1272, 1501 |
| Sau96I | GGNCC | 2 cut(s) | 380, 1210 |
| SchI | GAGTC | 1 cut(s) | 1013 |
| ScrFI | CCNGG | 3 cut(s) | 103, 453, 1550 |
| SduI | GDGCHC | 3 cut(s) | 140, 168, 1465 |
| SexAI | ACCWGGT | 1 cut(s) | 1548 |
| SfaNI | GCATC | 1 cut(s) | 1304 |
| SfcI | CTRYAG | 1 cut(s) | 937 |
| SgrAI | CRCCGGYG | 1 cut(s) | 179 |
| SinI | GGWCC | 2 cut(s) | 380, 1210 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 603 |
| SphI | GCATGC | 1 cut(s) | 1397 |
| Sse9I | AATT | 8 cut(s) | 91, 147, 468, 516, 564, 681, 783, 1633 |
| SsiI | CCGC | 6 cut(s) | 22, 70, 73, 153, 383, 534 |
| SspI | AATATT | 2 cut(s) | 296, 738 |
| SspMI | CTAG | 1 cut(s) | 1118 |
| StyD4I | CCNGG | 3 cut(s) | 101, 451, 1548 |
| StyI | CCWWGG | 2 cut(s) | 132, 619 |
| TaaI | ACNGT | 5 cut(s) | 18, 466, 713, 941, 1372 |
| TaiI | ACGT | 4 cut(s) | 198, 210, 376, 607 |
| TaqI | TCGA | 4 cut(s) | 30, 201, 309, 370 |
| TasI | AATT | 8 cut(s) | 91, 147, 468, 516, 564, 681, 783, 1633 |
| TatI | WGTACW | 2 cut(s) | 492, 1044 |
| TauI | GCSGC | 1 cut(s) | 75 |
| TfiI | GAWTC | 3 cut(s) | 997, 1141, 1178 |
| TscAI | CASTG | 3 cut(s) | 780, 847, 946 |
| TseFI | GTSAC | 2 cut(s) | 430, 1417 |
| TseI | GCWGC | 3 cut(s) | 24, 50, 875 |
| Tsp45I | GTSAC | 2 cut(s) | 430, 1417 |
| TspDTI | ATGAA | 2 cut(s) | 1154, 1328 |
| TspGWI | ACGGA | 2 cut(s) | 1265, 1436 |
| TspRI | CASTG | 3 cut(s) | 780, 847, 946 |
| Tth111I | GACNNNGTC | 1 cut(s) | 384 |
| VpaK11BI | GGWCC | 2 cut(s) | 380, 1210 |
| VspI | ATTAAT | 2 cut(s) | 684, 870 |
| XapI | RAATTY | 3 cut(s) | 147, 516, 783 |
| XceI | RCATGY | 4 cut(s) | 426, 438, 502, 1397 |
| XcmI | CCANNNNNNNNNTGG | 5 cut(s) | 139, 518, 605, 979, 1553 |
| XmiI | GTMKAC | 1 cut(s) | 1308 |
| XspI | CTAG | 1 cut(s) | 1118 |
| ZraI | GACGTC | 1 cut(s) | 208 |
| Zsp2I | ATGCAT | 1 cut(s) | 1297 |