Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000762

Physical Location & Seq

Genomic Coordinates Reverse (-)

21030097 .. 21035353

Gene Structure 5257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1180700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGCAATTTCCGATAACGGCGAGCCCAAAATCGCCACCGGCCCCTGCGGCTACGTCCTCGAAGATGTTCCTCACTTAGTTGATTACATCCCCGATCTCCCAACTTATCACAATCCATTACAAGACAATCCTTCCTATTCGGTTGTCAAGCAGTATTTCGTCAATGCGGATGATACCGTTGCCCAAAAGATTGTTGTCCACAAGGATAGTCCAAGGGGGACGCACTTTCGACGCTCTGGACCACGTCAGAAGGTGTATTTTGAGTCAAGTGAAGTGCACGCGTGTATCGTGACATGTGGTGGTCTATGTCCAGGACTGAATACAGTTATTAGAGAAATAGTATGCGGCCTGAACCACATGTATGGTGTCAAAAGAATTATCGGAATAGAGGTTGGATACAGAGGGTTTTATGCCAGAAATACAATCCCCTTAACGCCAAAGGTTGTGAATGACATCCATAAACGTGGTGGCACCATCCTTGGGACATCACGAGGCGGGCATGATACCTCAAAGATTGTGGACAGCATCCAGGACAGAGGAATTAATCAGGTTTATATACTTGGGGGAGACGGAACTCAGAGAGGAGCTTCGGTGATTTTTGAGGAAATCAGGAGGCGTGGCCTCAAAGTAGCGGTGGCAGGAATTCCAAAAACCATCGATAATGACATTCCGGTTATAGACAAATCTTTTGGTTTTGACACTGCTGTTGAGGAGGCTCAGCGAGCCATTAGTGCAGCCCATGTTGAAGCAGGAAGCATTGAAAATGGTATTGGTGTCGTCAAGTTAATGGGCCGCTATAGTGGATTCATAGCAATGTATGCTACTCTTGCTAGCCGAGACGTGGACTGTTGTCTGATTCCAGAATCACCTTTCTATCTGGAAGGACCAGGTGGACTTTATGAATATATTGAAAGAAGACTGAAGGAGAATGGTCACATGGTTATAGTAATAGCCGAAGGTGCAGGACAAGAGCTTCTTTCTGAAAGCATGAACCAGCAGGATGCTTCCGGAAATAAGCTTCTCCAAGATGTTGGTCTGTGGTTATCGCAAAATATTAGGGATCACTTCTTGAAACGGAGAAAGATGACTATAAATCTCAAATACATAGATCCCACTTACATGATCCGTGCTATTCCAAGCAATGCATCTGATAATGTATATTGCACCCTCCTTGCTCATAGTGCAATTCATGGTGCGATGGCTGGATACACGGGATTCACTGTCGGCCCTGTTAACGGAAGGCATGCTTACATTCCGTTCCATCGGGTTACCCGGGGGCAGAACAAGGTTGTGATAACTGACAGAATGTGGGCACGGTTATTGTCATCGACCAATCAGCCGAGCTTCTTGGACCTCAAAGAGGTCGAAGCAAAGATGAAAGAGGAAACAAAAGCCCAACTGATTGTTGGGGAAAACCATACTGAAGCGACTGAAGCGTCTAGACCAAAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230

Pfam Domains

Protein Families

PFK

Protein Analysis

485

Amino Acids

53.34

Weight (kDa)

7.26

Isoelectric Point (pI)

39.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	95 - 397	2.3e-60	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26270 AT4G29220 AT4G32840 AT5G56630
fragaria_vesca	FvH4_1g06570 FvH4_2g35190 FvH4_6g32610 FvH4_6g32610 FvH4_6g32610
malus_domestica	MD02G1068900.v1.1 MD08G1109700.v1.1 MD09G1200000.v1.1 MD15G1089400.v1.1 MD17G1180600.v1.1 MD17G1180700.v1.1 MD17G1180800.v1.1
prunus_persica	Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.7G216700_v2.0.a1
pyrus_communis	pycom02g05460 pycom07g22220 pycom09g11720 pycom15g08380 pycom17g19000 pycom17g19010 pycom17g19050
rosa_chinensis	RchiOBHm_Chr2g0092311 RchiOBHm_Chr2g0141061 RchiOBHm_Chr6g0306751
rosa_laevigata	RLG00000010768 RLG00000016296 RLG00000019912
rosa_multiflora	Rmu_sc0003289.1_g000020 Rmu_sc0010596.1_g000001 Rmu_sc0025856.1_g000001 Rmu_sc0032226.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104240 Rroxscaffold_2G00149270 Rroxscaffold_4G00313720 Rroxscaffold_7G00161490
rosa_rugosa	Rorug02G0024700 Rorug02G0366600 Rorug02G0366800 Rorug04G0176400 Rorug06G0352400 Rorug06G0352500
rosa_samantha	Rh2AG417900 Rh2BG070100 Rh2BG427800 Rh2CG071300 Rh2CG404200 Rh2DG069100 Rh2DG437400 Rh2DG437800 Rh4CG359100 Rh6AG464500 Rh6BG436300 Rh6CG478800 Rh6DG019000 Rh6DG465900 Rh6DG478600 Rh7DG340800
rosa_wichuraiana	Rw2G006170 Rw2G034210 Rw2G034270 Rw6G040480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1435
AccB1I	GGYRCC	1 cut(s)	470
AccII	CGCG	1 cut(s)	281
AccIII	TCCGGA	1 cut(s)	1007
AciI	CCGC	6 cut(s)	48, 167, 345, 495, 632, 793
AclWI	GGATC	3 cut(s)	1068, 1103, 1117
AcsI	RAATTY	1 cut(s)	642
AcuI	CTGAAG	3 cut(s)	941, 1443, 1452
AfiI	CCNNNNNNNGG	4 cut(s)	480, 841, 1235, 1360
AflIII	ACRYGT	3 cut(s)	279, 293, 357
AgsI	TTSAA	4 cut(s)	746, 761, 911, 1072
AjiI	CACGTC	2 cut(s)	245, 841
AjnI	CCWGG	3 cut(s)	310, 528, 886
AjuI	GAANNNNNNNTTGG	2 cut(s)	753, 785
AluBI	AGCT	4 cut(s)	587, 973, 1018, 1344
AluI	AGCT	4 cut(s)	587, 973, 1018, 1344
Alw21I	GWGCWC	1 cut(s)	279
Alw26I	GTCTC	2 cut(s)	561, 831
Alw44I	GTGCAC	1 cut(s)	275
AlwI	GGATC	3 cut(s)	1068, 1103, 1117
Ama87I	CYCGRG	1 cut(s)	1272
Aor13HI	TCCGGA	1 cut(s)	1007
AoxI	GGCC	5 cut(s)	40, 346, 619, 790, 1225
ApaLI	GTGCAC	1 cut(s)	275
ApeKI	GCWGC	1 cut(s)	734
ApoI	RAATTY	1 cut(s)	642
ArsI	GACNNNNNNTTYG	2 cut(s)	671, 703
AseI	ATTAAT	1 cut(s)	543
Asp700I	GAANNNNTTC	1 cut(s)	66
AspS9I	GGNCC	6 cut(s)	41, 239, 790, 884, 1226, 1351
AsuC2I	CCSGG	2 cut(s)	1273, 1274
AsuHPI	GGTGA	2 cut(s)	604, 858
AsuNHI	GCTAGC	1 cut(s)	830
AvaI	CYCGRG	1 cut(s)	1272
AvaII	GGWCC	3 cut(s)	239, 884, 1351
BaeGI	GKGCMC	2 cut(s)	279, 1315
BaeI	ACNNNNGTAYC	2 cut(s)	268, 301
BanI	GGYRCC	1 cut(s)	470
BanII	GRGCYC	1 cut(s)	27
BauI	CACGAG	1 cut(s)	489
BbsI	GAAGAC	1 cut(s)	922
Bbv12I	GWGCWC	1 cut(s)	279
BbvI	GCAGC	1 cut(s)	746
BccI	CCATC	4 cut(s)	482, 662, 1192, 1269
BceAI	ACGGC	1 cut(s)	34
BciT130I	CCWGG	3 cut(s)	312, 530, 888
BciVI	GTATCC	2 cut(s)	389, 1199
BcnI	CCSGG	2 cut(s)	1273, 1274
BcoDI	GTCTC	2 cut(s)	561, 831
BfaI	CTAG	2 cut(s)	831, 1440
BfmI	CTRYAG	1 cut(s)	796
BfuI	GTATCC	2 cut(s)	389, 1199
BglI	GCCNNNNNGGC	1 cut(s)	48
BisI	GCNGC	4 cut(s)	49, 346, 735, 793
BlpI	GCTNAGC	1 cut(s)	717
BlsI	GCNGC	4 cut(s)	50, 347, 736, 794
Bme1390I	CCNGG	5 cut(s)	312, 530, 888, 1273, 1274
Bme18I	GGWCC	3 cut(s)	239, 884, 1351
BmeT110I	CYCGRG	1 cut(s)	1272
BmgBI	CACGTC	2 cut(s)	245, 841
BmgT120I	GGNCC	6 cut(s)	41, 239, 790, 884, 1226, 1351
BmiI	GGNNCC	2 cut(s)	43, 472
BmrFI	CCNGG	5 cut(s)	312, 530, 888, 1273, 1274
BmsI	GCATC	3 cut(s)	534, 991, 1154
BmtI	GCTAGC	1 cut(s)	834
BoxI	GACNNNNGTC	1 cut(s)	849
BpiI	GAAGAC	1 cut(s)	922
Bpu1102I	GCTNAGC	1 cut(s)	717
BpuMI	CCSGG	2 cut(s)	1273, 1274
Bsa29I	ATCGAT	1 cut(s)	657
BsaJI	CCNNGG	4 cut(s)	212, 478, 1272, 1273
BsaWI	WCCGGW	2 cut(s)	670, 1007
BsaXI	ACNNNNNCTCC	2 cut(s)	576, 606
Bsc4I	CCNNNNNNNGG	4 cut(s)	480, 841, 1235, 1360
Bse118I	RCCGGY	1 cut(s)	38
Bse3DI	GCAATG	2 cut(s)	819, 1147
BseAI	TCCGGA	1 cut(s)	1007
BseBI	CCWGG	3 cut(s)	312, 530, 888
BseCI	ATCGAT	1 cut(s)	657
BseDI	CCNNGG	4 cut(s)	212, 478, 1272, 1273
BseGI	GGATG	6 cut(s)	87, 175, 453, 474, 525, 1006
BseLI	CCNNNNNNNGG	4 cut(s)	480, 841, 1235, 1360
BseMI	GCAATG	2 cut(s)	819, 1147
BseMII	CTCAG	2 cut(s)	590, 731
BseRI	GAGGAG	2 cut(s)	597, 725
BseSI	GKGCMC	2 cut(s)	279, 1315
BseXI	GCAGC	1 cut(s)	746
BsgI	GTGCAG	2 cut(s)	753, 981
Bsh1236I	CGCG	1 cut(s)	281
BshFI	GGCC	5 cut(s)	42, 348, 621, 792, 1227
BshNI	GGYRCC	1 cut(s)	470
BshVI	ATCGAT	1 cut(s)	657
BsiHKAI	GWGCWC	1 cut(s)	279
BsiHKCI	CYCGRG	1 cut(s)	1272
BsiSI	CCGG	4 cut(s)	39, 671, 1008, 1273
BslFI	GGGAC	2 cut(s)	232, 496
BslI	CCNNNNNNNGG	4 cut(s)	480, 841, 1235, 1360
BsmAI	GTCTC	2 cut(s)	561, 831
BsmBI	CGTCTC	2 cut(s)	561, 831
BsmFI	GGGAC	2 cut(s)	232, 496
BsnI	GGCC	5 cut(s)	42, 348, 621, 792, 1227
BsoBI	CYCGRG	1 cut(s)	1272
Bsp1286I	GDGCHC	3 cut(s)	27, 279, 1315
Bsp13I	TCCGGA	1 cut(s)	1007
Bsp143I	GATC	4 cut(s)	94, 1060, 1108, 1122
Bsp1720I	GCTNAGC	1 cut(s)	717
BspACI	CCGC	6 cut(s)	48, 167, 345, 495, 632, 793
BspANI	GGCC	5 cut(s)	42, 348, 621, 792, 1227
BspCNI	CTCAG	2 cut(s)	589, 730
BspDI	ATCGAT	1 cut(s)	657
BspEI	TCCGGA	1 cut(s)	1007
BspFNI	CGCG	1 cut(s)	281
BspLI	GGNNCC	2 cut(s)	43, 472
BspOI	GCTAGC	1 cut(s)	834
BspPI	GGATC	3 cut(s)	1068, 1103, 1117
BspT107I	GGYRCC	1 cut(s)	470
BsrDI	GCAATG	2 cut(s)	819, 1147
BsrFI	RCCGGY	1 cut(s)	38
BssAI	RCCGGY	1 cut(s)	38
BssECI	CCNNGG	4 cut(s)	212, 478, 1272, 1273
BssMI	GATC	4 cut(s)	94, 1060, 1108, 1122
BssSI	CACGAG	1 cut(s)	489
BssT1I	CCWWGG	2 cut(s)	212, 478
Bst2BI	CACGAG	1 cut(s)	489
Bst2UI	CCWGG	3 cut(s)	312, 530, 888
Bst4CI	ACNGT	5 cut(s)	178, 325, 848, 1222, 1317
BstAPI	GCANNNNNTGC	1 cut(s)	818
BstC8I	GCNNGC	6 cut(s)	23, 279, 497, 723, 832, 1245
BstDEI	CTNAG	3 cut(s)	76, 576, 717
BstEII	GGTNACC	1 cut(s)	1267
BstENI	CCTNNNNNAGG	1 cut(s)	1358
BstF5I	GGATG	6 cut(s)	87, 175, 453, 474, 525, 1006
BstFNI	CGCG	1 cut(s)	281
BstKTI	GATC	4 cut(s)	97, 1063, 1111, 1125
BstMAI	GTCTC	2 cut(s)	561, 831
BstMBI	GATC	4 cut(s)	94, 1060, 1108, 1122
BstMWI	GCNNNNNNNGC	8 cut(s)	48, 722, 731, 818, 827, 959, 1181, 1433
BstNI	CCWGG	3 cut(s)	312, 530, 888
BstNSI	RCATGY	3 cut(s)	297, 361, 1247
BstPAI	GACNNNNGTC	1 cut(s)	849
BstPI	GGTNACC	1 cut(s)	1267
BstSCI	CCNGG	5 cut(s)	310, 528, 886, 1271, 1272
BstSFI	CTRYAG	1 cut(s)	796
BstSLI	GKGCMC	2 cut(s)	279, 1315
BstUI	CGCG	1 cut(s)	281
BstV1I	GCAGC	1 cut(s)	746
BstV2I	GAAGAC	1 cut(s)	922
BstX2I	RGATCY	1 cut(s)	1108
BstXI	CCANNNNNNTGG	3 cut(s)	362, 464, 1031
BstYI	RGATCY	1 cut(s)	1108
Bsu15I	ATCGAT	1 cut(s)	657
BsuI	GTATCC	2 cut(s)	389, 1199
BsuRI	GGCC	5 cut(s)	42, 348, 621, 792, 1227
BsuTUI	ATCGAT	1 cut(s)	657
BtgZI	GCGATG	1 cut(s)	1211
BtrI	CACGTC	2 cut(s)	245, 841
BtsCI	GGATG	6 cut(s)	87, 175, 453, 474, 525, 1006
BtsI	GCAGTG	1 cut(s)	699
BtsIMutI	CAGTG	2 cut(s)	699, 1218
Cac8I	GCNNGC	6 cut(s)	23, 279, 497, 723, 832, 1245
Cfr10I	RCCGGY	1 cut(s)	38
Cfr13I	GGNCC	6 cut(s)	41, 239, 790, 884, 1226, 1351
Cfr9I	CCCGGG	1 cut(s)	1272
ClaI	ATCGAT	1 cut(s)	657
CseI	GACGC	3 cut(s)	229, 240, 1425
CsiI	ACCWGGT	1 cut(s)	886
CspCI	CAANNNNNGTGG	2 cut(s)	498, 533
CviAII	CATG	9 cut(s)	294, 358, 500, 740, 937, 988, 1120, 1190, 1244
DdeI	CTNAG	3 cut(s)	76, 576, 717
DpnI	GATC	4 cut(s)	96, 1062, 1110, 1124
DpnII	GATC	4 cut(s)	94, 1060, 1108, 1122
DrdI	GACNNNNNNGTC	1 cut(s)	1435
DseDI	GACNNNNNNGTC	1 cut(s)	1435
Eco130I	CCWWGG	2 cut(s)	212, 478
Eco24I	GRGCYC	1 cut(s)	27
Eco47I	GGWCC	3 cut(s)	239, 884, 1351
Eco57I	CTGAAG	3 cut(s)	941, 1443, 1452
Eco88I	CYCGRG	1 cut(s)	1272
Eco91I	GGTNACC	1 cut(s)	1267
EcoNI	CCTNNNNNAGG	1 cut(s)	1358
EcoO65I	GGTNACC	1 cut(s)	1267
EcoRI	GAATTC	1 cut(s)	642
EcoRII	CCWGG	3 cut(s)	310, 528, 886
EcoT14I	CCWWGG	2 cut(s)	212, 478
EcoT22I	ATGCAT	1 cut(s)	1147
EcoT38I	GRGCYC	1 cut(s)	27
ErhI	CCWWGG	2 cut(s)	212, 478
Esp3I	CGTCTC	2 cut(s)	561, 831
FaeI	CATG	9 cut(s)	297, 361, 503, 743, 940, 991, 1123, 1193, 1247
FalI	AAGNNNNNCTT	4 cut(s)	960, 992, 1231, 1263
FaqI	GGGAC	2 cut(s)	232, 496
FatI	CATG	9 cut(s)	293, 357, 499, 739, 936, 987, 1119, 1189, 1243
FauI	CCCGC	1 cut(s)	488
Fnu4HI	GCNGC	4 cut(s)	49, 346, 735, 793
FokI	GGATG	6 cut(s)	74, 182, 440, 461, 512, 1013
FriOI	GRGCYC	1 cut(s)	27
Fsp4HI	GCNGC	4 cut(s)	49, 346, 735, 793
FspBI	CTAG	2 cut(s)	831, 1440
GluI	GCNGC	4 cut(s)	49, 346, 735, 793
HaeIII	GGCC	5 cut(s)	42, 348, 621, 792, 1227
HapII	CCGG	4 cut(s)	39, 671, 1008, 1273
HgaI	GACGC	3 cut(s)	229, 240, 1425
Hin1II	CATG	9 cut(s)	297, 361, 503, 743, 940, 991, 1123, 1193, 1247
HincII	GTYRAC	1 cut(s)	1234
HindII	GTYRAC	1 cut(s)	1234
HindIII	AAGCTT	1 cut(s)	1016
HinfI	GANTC	5 cut(s)	263, 804, 856, 863, 1215
HpaI	GTTAAC	1 cut(s)	1234
HpaII	CCGG	4 cut(s)	39, 671, 1008, 1273
HphI	GGTGA	2 cut(s)	604, 858
Hpy166II	GTNNAC	6 cut(s)	199, 277, 520, 844, 893, 1234
Hpy188I	TCNGA	7 cut(s)	13, 249, 383, 579, 855, 982, 1150
Hpy188III	TCNNGA	9 cut(s)	237, 289, 489, 610, 860, 878, 1008, 1069, 1440
Hpy8I	GTNNAC	6 cut(s)	199, 277, 520, 844, 893, 1234
Hpy99I	CGWCG	1 cut(s)	234
HpyAV	CCTTC	6 cut(s)	141, 244, 875, 916, 950, 1233
HpyCH4III	ACNGT	5 cut(s)	178, 325, 848, 1222, 1317
HpyCH4IV	ACGT	4 cut(s)	54, 244, 463, 840
HpyCH4V	TGCA	6 cut(s)	277, 734, 962, 1145, 1164, 1184
HpyF10VI	GCNNNNNNNGC	8 cut(s)	48, 722, 731, 818, 827, 959, 1181, 1433
HpyF3I	CTNAG	3 cut(s)	76, 576, 717
HpySE526I	ACGT	4 cut(s)	54, 244, 463, 840
Hsp92II	CATG	9 cut(s)	297, 361, 503, 743, 940, 991, 1123, 1193, 1247
Kpn2I	TCCGGA	1 cut(s)	1007
KspAI	GTTAAC	1 cut(s)	1234
Kzo9I	GATC	4 cut(s)	94, 1060, 1108, 1122
LmnI	GCTCC	1 cut(s)	584
Lsp1109I	GCAGC	1 cut(s)	746
LweI	GCATC	3 cut(s)	534, 991, 1154
MabI	ACCWGGT	1 cut(s)	886
MaeI	CTAG	2 cut(s)	831, 1440
MaeII	ACGT	4 cut(s)	54, 244, 463, 840
MaeIII	GTNAC	3 cut(s)	289, 932, 1267
MalI	GATC	4 cut(s)	96, 1062, 1110, 1124
MboI	GATC	4 cut(s)	94, 1060, 1108, 1122
MboII	GAAGA	2 cut(s)	74, 927
MflI	RGATCY	1 cut(s)	1108
MhlI	GDGCHC	3 cut(s)	27, 279, 1315
MluCI	AATT	5 cut(s)	6, 375, 540, 642, 1185
MluI	ACGCGT	1 cut(s)	279
MlyI	GAGTC	1 cut(s)	272
MmeI	TCCRAC	1 cut(s)	373
Mph1103I	ATGCAT	1 cut(s)	1147
MroI	TCCGGA	1 cut(s)	1007
MroXI	GAANNNNTTC	1 cut(s)	66
MseI	TTAA	4 cut(s)	431, 543, 785, 1233
MslI	CAYNNNNRTG	4 cut(s)	360, 462, 812, 1118
MspI	CCGG	4 cut(s)	39, 671, 1008, 1273
MspR9I	CCNGG	5 cut(s)	312, 530, 888, 1273, 1274
MvaI	CCWGG	3 cut(s)	312, 530, 888
MvnI	CGCG	1 cut(s)	281
MwoI	GCNNNNNNNGC	8 cut(s)	48, 722, 731, 818, 827, 959, 1181, 1433
NciI	CCSGG	2 cut(s)	1273, 1274
NdeII	GATC	4 cut(s)	94, 1060, 1108, 1122
NheI	GCTAGC	1 cut(s)	830
NlaIII	CATG	9 cut(s)	297, 361, 503, 743, 940, 991, 1123, 1193, 1247
NlaIV	GGNNCC	2 cut(s)	43, 472
NmeAIII	GCCGAG	2 cut(s)	860, 1365
NmuCI	GTSAC	2 cut(s)	289, 932
NsiI	ATGCAT	1 cut(s)	1147
NspI	RCATGY	3 cut(s)	297, 361, 1247
PaeI	GCATGC	1 cut(s)	1247
PciI	ACATGT	2 cut(s)	293, 357
PcsI	WCGNNNNNNNCGW	1 cut(s)	285
PdmI	GAANNNNTTC	1 cut(s)	66
PfeI	GAWTC	4 cut(s)	804, 856, 863, 1215
PflFI	GACNNNGTC	1 cut(s)	243
PfoI	TCCNGGA	2 cut(s)	310, 528
PkrI	GCNGC	4 cut(s)	50, 347, 736, 794
PleI	GAGTC	1 cut(s)	271
PpsI	GAGTC	1 cut(s)	271
PscI	ACATGT	2 cut(s)	293, 357
PshAI	GACNNNNGTC	1 cut(s)	849
PshBI	ATTAAT	1 cut(s)	543
Psp6I	CCWGG	3 cut(s)	310, 528, 886
PspEI	GGTNACC	1 cut(s)	1267
PspGI	CCWGG	3 cut(s)	310, 528, 886
PspN4I	GGNNCC	2 cut(s)	43, 472
PspPI	GGNCC	6 cut(s)	41, 239, 790, 884, 1226, 1351
PsuI	RGATCY	1 cut(s)	1108
PsyI	GACNNNGTC	1 cut(s)	243
RseI	CAYNNNNRTG	4 cut(s)	360, 462, 812, 1118
SaqAI	TTAA	4 cut(s)	431, 543, 785, 1233
SatI	GCNGC	4 cut(s)	49, 346, 735, 793
Sau3AI	GATC	4 cut(s)	94, 1060, 1108, 1122
Sau96I	GGNCC	6 cut(s)	41, 239, 790, 884, 1226, 1351
SchI	GAGTC	1 cut(s)	272
ScrFI	CCNGG	5 cut(s)	312, 530, 888, 1273, 1274
SduI	GDGCHC	3 cut(s)	27, 279, 1315
SexAI	ACCWGGT	1 cut(s)	886
SfaNI	GCATC	3 cut(s)	534, 991, 1154
SfcI	CTRYAG	1 cut(s)	796
SinI	GGWCC	3 cut(s)	239, 884, 1351
SmaI	CCCGGG	1 cut(s)	1274
SmiMI	CAYNNNNRTG	4 cut(s)	360, 462, 812, 1118
SphI	GCATGC	1 cut(s)	1247
Sse9I	AATT	5 cut(s)	6, 375, 540, 642, 1185
SsiI	CCGC	6 cut(s)	48, 167, 345, 495, 632, 793
SspI	AATATT	1 cut(s)	1054
SspMI	CTAG	2 cut(s)	831, 1440
StyD4I	CCNGG	5 cut(s)	310, 528, 886, 1271, 1272
StyI	CCWWGG	2 cut(s)	212, 478
TaaI	ACNGT	5 cut(s)	178, 325, 848, 1222, 1317
TaiI	ACGT	4 cut(s)	57, 247, 466, 843
TaqI	TCGA	5 cut(s)	60, 229, 657, 1328, 1365
TasI	AATT	5 cut(s)	6, 375, 540, 642, 1185
TauI	GCSGC	3 cut(s)	51, 348, 795
TfiI	GAWTC	4 cut(s)	804, 856, 863, 1215
Tru1I	TTAA	4 cut(s)	431, 543, 785, 1233
Tru9I	TTAA	4 cut(s)	431, 543, 785, 1233
TscAI	CASTG	2 cut(s)	706, 1225
TseFI	GTSAC	2 cut(s)	289, 932
TseI	GCWGC	1 cut(s)	734
Tsp45I	GTSAC	2 cut(s)	289, 932
TspDTI	ATGAA	5 cut(s)	796, 915, 1004, 1178, 1391
TspGWI	ACGGA	5 cut(s)	585, 1090, 1115, 1245, 1251
TspMI	CCCGGG	1 cut(s)	1272
TspRI	CASTG	2 cut(s)	706, 1225
Tth111I	GACNNNGTC	1 cut(s)	243
VneI	GTGCAC	1 cut(s)	275
VpaK11BI	GGWCC	3 cut(s)	239, 884, 1351
VspI	ATTAAT	1 cut(s)	543
XagI	CCTNNNNNAGG	1 cut(s)	1358
XapI	RAATTY	1 cut(s)	642
XbaI	TCTAGA	1 cut(s)	1439
XceI	RCATGY	3 cut(s)	297, 361, 1247
XcmI	CCANNNNNNNNNTGG	2 cut(s)	464, 1403
XmaI	CCCGGG	1 cut(s)	1272
XmnI	GAANNNNTTC	1 cut(s)	66
XspI	CTAG	2 cut(s)	831, 1440
Zsp2I	ATGCAT	1 cut(s)	1147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.