Rmu_sc0010596.1_g000001

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010596.1
OG0000762
Physical Location & Seq
Forward (+)
140 .. 3875
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010596.1_g000001.1.cds

Sequence Viewer

Length: 1449 bp
atggcggtttccggtgatctgaagatgaagctcgtcaaaggcgacgccggttacgttctcgaagatgtgcctcatctcgccgattacttgtccgatcttcctacttatcccaatccactgcaaatcaatcctgcttactctgtagtcaagcagtattttgttaaccaagatgacactgttgctcaaaagattgttgtgcacaaggatagtccaagagggacacaccttcggcgagcaggaccacgccaaaaggtgtattttgaggcggatgaagtgcatgcctgtattgtgacttgtggtggtttgtgccctgggctcaacacggtgatcagggagattgtctgtggactttatcacatgtatggtgtcaccagggttcttggaatagatggaggatatagtggtttctattcccgaaatactattgagttgacacctagggttgtgaatgacatccacaaacgtggtggtactatccttgggacatcacgaggaggccatgatacctcgaagatagttgacagcattcatgatcgtggaattaaccatgtttacataattggaggtgatggaactcaaaaaggagcatctgtgatatttgaggaagttagacgccgtggcctcaaagtttctgttgtaggagttcctaaaacaatagacaatgacataccggttatagatagatcctttggctttgatactgcggttgaggaggcccagcgtgccatatgtgcagctcatgtggaagctgaaagtattgagcatggtattggtcttgtgaagctaatgggacgctacagcggtttcatagctatgtatgctacacttgccagtcgagatgtggactgttgtttgattccagagtcccccttctttcttgaaggacgaggtggactttttgagtatattgaaaaacgactcaaagaaaatggacatatggttattgtgcttgctgagggagcaggacaggatcttctttcagagagcttgcaatccatgaaccagcaagatgcttcaggaaacaagctacttcaagatgttgggttatggatatctcagaggatcaaggatcactttgcaagacaacaaaagatgtctattaatctgaaatatatagatcctacatacatgatcagggctattccaagtaatgcagctgacaatgtctactgcacactgctggctcaaagtgccgttcatggagcaatggcagggttcacgggctttgtagttgggcctgtcaatggaagacattcttacattccttttaatcatattactgagaaacagaacaaggttgtgattaccgataggatgtgggcacgactcctttcttcaactaaccagccgagcttcttgaaccccaaagacgttgatgaagtccaaaaagaggaagagcctccaactcaactgctagaaggggaaaattgtcaagatagcaagtactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

482

Amino Acids

53.18

Weight (kDa)

6.16

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1167
AciI CCGC 4 cut(s) 5, 266, 704, 801
AclWI GGATC 5 cut(s) 678, 978, 1070, 1077, 1112
AcuI CTGAAG 2 cut(s) 41, 999
AcyI GRCGYC 2 cut(s) 45, 613
AfaI GTAC 2 cut(s) 472, 1445
AfiI CCNNNNNNNGG 2 cut(s) 1244, 1390
AflIII ACRYGT 1 cut(s) 357
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 5 cut(s) 881, 911, 1034, 1338, 1360
AjnI CCWGG 2 cut(s) 310, 371
AleI CACNNNNGTG 1 cut(s) 462
AluBI AGCT 9 cut(s) 31, 737, 749, 784, 812, 987, 1027, 1157, 1353
AluI AGCT 9 cut(s) 31, 737, 749, 784, 812, 987, 1027, 1157, 1353
Alw21I GWGCWC 1 cut(s) 201
Alw44I GTGCAC 1 cut(s) 197
AlwI GGATC 5 cut(s) 678, 978, 1070, 1077, 1112
AoxI GGCC 4 cut(s) 496, 619, 714, 1235
ApaLI GTGCAC 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 734, 1154
AseI ATTAAT 1 cut(s) 1101
AsiGI ACCGGT 1 cut(s) 670
Asp700I GAANNNNTTC 1 cut(s) 1252
AspA2I CCTAGG 1 cut(s) 437
AspS9I GGNCC 3 cut(s) 239, 715, 1235
AsuHPI GGTGA 4 cut(s) 26, 337, 361, 578
AvaII GGWCC 1 cut(s) 239
AvrII CCTAGG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 201, 311, 1324
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BanII GRGCYC 1 cut(s) 318
BauI CACGAG 1 cut(s) 489
BbsI GAAGAC 1 cut(s) 1255
Bbv12I GWGCWC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 2 cut(s) 746, 1166
BccI CCATC 2 cut(s) 383, 563
BceAI ACGGC 2 cut(s) 600, 1178
BciT130I CCWGG 2 cut(s) 312, 373
BclI TGATCA 2 cut(s) 327, 1131
BfaI CTAG 2 cut(s) 438, 1415
BfmI CTRYAG 2 cut(s) 141, 796
BisI GCNGC 2 cut(s) 735, 1155
BlnI CCTAGG 1 cut(s) 437
BlsI GCNGC 2 cut(s) 736, 1156
BmcAI AGTACT 1 cut(s) 1445
Bme1390I CCNGG 2 cut(s) 312, 373
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 3 cut(s) 239, 715, 1235
BmrFI CCNGG 2 cut(s) 312, 373
BmsI GCATC 2 cut(s) 596, 1000
BpiI GAAGAC 1 cut(s) 1255
Bpu10I CCTNAGC 1 cut(s) 954
BsaHI GRCGYC 2 cut(s) 45, 613
BsaJI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BsaWI WCCGGW 2 cut(s) 11, 670
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 1244, 1390
Bse118I RCCGGY 2 cut(s) 47, 670
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 312, 373
BseDI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BseGI GGATG 3 cut(s) 274, 453, 1320
BseLI CCNNNNNNNGG 2 cut(s) 1244, 1390
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 3 cut(s) 945, 1070, 1272
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 2 cut(s) 507, 725
BseSI GKGCMC 3 cut(s) 201, 311, 1324
BseXI GCAGC 2 cut(s) 746, 1166
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 2 cut(s) 753, 1156
BshFI GGCC 4 cut(s) 498, 621, 716, 1237
BshTI ACCGGT 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 3 cut(s) 12, 48, 671
BslFI GGGAC 4 cut(s) 232, 496, 804, 850
BslI CCNNNNNNNGG 2 cut(s) 1244, 1390
BsmFI GGGAC 4 cut(s) 232, 496, 804, 850
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 4 cut(s) 498, 621, 716, 1237
Bsp1286I GDGCHC 4 cut(s) 201, 311, 318, 1324
BspACI CCGC 4 cut(s) 5, 266, 704, 801
BspANI GGCC 4 cut(s) 498, 621, 716, 1237
BspCNI CTCAG 3 cut(s) 946, 1069, 1273
BspHI TCATGA 1 cut(s) 529
BspPI GGATC 5 cut(s) 678, 978, 1070, 1077, 1112
BspQI GCTCTTC 1 cut(s) 1389
BsrDI GCAATG 1 cut(s) 1212
BsrFI RCCGGY 2 cut(s) 47, 670
BsrI ACTGG 1 cut(s) 831
BssAI RCCGGY 2 cut(s) 47, 670
BssECI CCNNGG 6 cut(s) 310, 311, 372, 437, 478, 616
BssNI GRCGYC 2 cut(s) 45, 613
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 437, 478
Bst2BI CACGAG 1 cut(s) 489
Bst2UI CCWGG 2 cut(s) 312, 373
Bst4CI ACNGT 3 cut(s) 178, 325, 848
Bst6I CTCTTC 1 cut(s) 1389
BstACI GRCGYC 2 cut(s) 45, 613
BstC8I GCNNGC 6 cut(s) 234, 279, 723, 951, 989, 1182
BstDEI CTNAG 3 cut(s) 954, 1056, 1281
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 3 cut(s) 274, 453, 1320
BstMWI GCNNNNNNNGC 6 cut(s) 722, 731, 818, 827, 959, 1190
BstNI CCWGG 2 cut(s) 312, 373
BstNSI RCATGY 2 cut(s) 281, 361
BstSCI CCNGG 2 cut(s) 310, 371
BstSFI CTRYAG 2 cut(s) 141, 796
BstSLI GKGCMC 3 cut(s) 201, 311, 1324
BstV1I GCAGC 2 cut(s) 746, 1166
BstV2I GAAGAC 1 cut(s) 1255
BstX2I RGATCY 3 cut(s) 683, 970, 1117
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 3 cut(s) 683, 970, 1117
BsuRI GGCC 4 cut(s) 498, 621, 716, 1237
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 3 cut(s) 274, 453, 1320
BtsI GCAGTG 2 cut(s) 116, 1175
BtsIMutI CAGTG 3 cut(s) 116, 174, 1175
Cac8I GCNNGC 6 cut(s) 234, 279, 723, 951, 989, 1182
CciI TCATGA 1 cut(s) 529
Cfr10I RCCGGY 2 cut(s) 47, 670
Cfr13I GGNCC 3 cut(s) 239, 715, 1235
CseI GACGC 3 cut(s) 53, 621, 801
Csp6I GTAC 2 cut(s) 471, 1444
CspAI ACCGGT 1 cut(s) 670
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviQI GTAC 2 cut(s) 471, 1444
DdeI CTNAG 3 cut(s) 954, 1056, 1281
Eam1104I CTCTTC 1 cut(s) 1389
EarI CTCTTC 1 cut(s) 1389
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 437, 478
Eco24I GRGCYC 1 cut(s) 318
Eco32I GATATC 1 cut(s) 1053
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 41, 999
EcoRII CCWGG 2 cut(s) 310, 371
EcoRV GATATC 1 cut(s) 1053
EcoT14I CCWWGG 2 cut(s) 437, 478
EcoT38I GRGCYC 1 cut(s) 318
ErhI CCWWGG 2 cut(s) 437, 478
FalI AAGNNNNNCTT 4 cut(s) 1058, 1090, 1240, 1272
FaqI GGGAC 4 cut(s) 232, 496, 804, 850
FauNDI CATATG 2 cut(s) 728, 936
FbaI TGATCA 2 cut(s) 327, 1131
FblI GTMKAC 1 cut(s) 1167
Fnu4HI GCNGC 2 cut(s) 735, 1155
FokI GGATG 3 cut(s) 281, 440, 1327
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 2 cut(s) 735, 1155
FspBI CTAG 2 cut(s) 438, 1415
GluI GCNGC 2 cut(s) 735, 1155
GsaI CCCAGC 1 cut(s) 721
HaeIII GGCC 4 cut(s) 498, 621, 716, 1237
HapII CCGG 3 cut(s) 12, 48, 671
HgaI GACGC 3 cut(s) 53, 621, 801
Hin1I GRCGYC 2 cut(s) 45, 613
HincII GTYRAC 3 cut(s) 163, 432, 520
HindII GTYRAC 3 cut(s) 163, 432, 520
HinfI GANTC 4 cut(s) 856, 863, 918, 1326
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 3 cut(s) 12, 48, 671
HphI GGTGA 4 cut(s) 26, 337, 361, 578
Hpy188I TCNGA 5 cut(s) 21, 94, 982, 1059, 1107
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 4 cut(s) 236, 875, 880, 1412
HpyCH4III ACNGT 3 cut(s) 178, 325, 848
HpyCH4IV ACGT 3 cut(s) 54, 463, 1371
HpyCH4V TGCA 8 cut(s) 121, 199, 277, 734, 991, 1079, 1154, 1173
HpyF10VI GCNNNNNNNGC 6 cut(s) 722, 731, 818, 827, 959, 1190
HpyF3I CTNAG 3 cut(s) 954, 1056, 1281
HpySE526I ACGT 3 cut(s) 54, 463, 1371
Hsp92I GRCGYC 2 cut(s) 45, 613
Ksp22I TGATCA 2 cut(s) 327, 1131
KspAI GTTAAC 1 cut(s) 163
LguI GCTCTTC 1 cut(s) 1389
LmnI GCTCC 3 cut(s) 584, 959, 1202
Lsp1109I GCAGC 2 cut(s) 746, 1166
LweI GCATC 2 cut(s) 596, 1000
MaeI CTAG 2 cut(s) 438, 1415
MaeII ACGT 3 cut(s) 54, 463, 1371
MaeIII GTNAC 3 cut(s) 50, 289, 367
MboII GAAGA 8 cut(s) 34, 74, 89, 523, 965, 1260, 1326, 1406
MflI RGATCY 3 cut(s) 683, 970, 1117
MhlI GDGCHC 4 cut(s) 201, 311, 318, 1324
MluCI AATT 3 cut(s) 540, 558, 1426
MlyI GAGTC 3 cut(s) 872, 912, 1320
MmeI TCCRAC 1 cut(s) 1427
MroXI GAANNNNTTC 1 cut(s) 1252
MseI TTAA 4 cut(s) 162, 543, 1101, 1269
MslI CAYNNNNRTG 4 cut(s) 360, 462, 534, 812
MspA1I CMGCKG 2 cut(s) 801, 1157
MspI CCGG 3 cut(s) 12, 48, 671
MspR9I CCNGG 2 cut(s) 312, 373
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 2 cut(s) 312, 373
MwoI GCNNNNNNNGC 6 cut(s) 722, 731, 818, 827, 959, 1190
NdeI CATATG 2 cut(s) 728, 936
NmeAIII GCCGAG 1 cut(s) 1374
NmuCI GTSAC 2 cut(s) 289, 367
NspI RCATGY 2 cut(s) 281, 361
OliI CACNNNNGTG 1 cut(s) 462
PaeI GCATGC 1 cut(s) 281
PagI TCATGA 1 cut(s) 529
PasI CCCWGGG 1 cut(s) 311
PciI ACATGT 1 cut(s) 357
PciSI GCTCTTC 1 cut(s) 1389
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 51
PctI GAATGC 1 cut(s) 525
PdmI GAANNNNTTC 1 cut(s) 1252
PfeI GAWTC 1 cut(s) 856
PflFI GACNNNGTC 1 cut(s) 1163
PinAI ACCGGT 1 cut(s) 670
PkrI GCNGC 2 cut(s) 736, 1156
PleI GAGTC 3 cut(s) 871, 912, 1320
PpsI GAGTC 3 cut(s) 871, 912, 1320
PscI ACATGT 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 1101
Psp6I CCWGG 2 cut(s) 310, 371
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 2 cut(s) 310, 371
PspPI GGNCC 3 cut(s) 239, 715, 1235
PsuI RGATCY 3 cut(s) 683, 970, 1117
PsyI GACNNNGTC 1 cut(s) 1163
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 2 cut(s) 472, 1445
RsaNI GTAC 2 cut(s) 471, 1444
RseI CAYNNNNRTG 4 cut(s) 360, 462, 534, 812
SapI GCTCTTC 1 cut(s) 1389
SaqAI TTAA 4 cut(s) 162, 543, 1101, 1269
SatI GCNGC 2 cut(s) 735, 1155
Sau96I GGNCC 3 cut(s) 239, 715, 1235
ScaI AGTACT 1 cut(s) 1445
SchI GAGTC 3 cut(s) 872, 912, 1320
ScrFI CCNGG 2 cut(s) 312, 373
SduI GDGCHC 4 cut(s) 201, 311, 318, 1324
SfaNI GCATC 2 cut(s) 596, 1000
SfcI CTRYAG 2 cut(s) 141, 796
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 4 cut(s) 360, 462, 534, 812
SphI GCATGC 1 cut(s) 281
Sse9I AATT 3 cut(s) 540, 558, 1426
SsiI CCGC 4 cut(s) 5, 266, 704, 801
SspMI CTAG 2 cut(s) 438, 1415
StyD4I CCNGG 2 cut(s) 310, 371
StyI CCWWGG 2 cut(s) 437, 478
TaaI ACNGT 3 cut(s) 178, 325, 848
TaiI ACGT 3 cut(s) 57, 466, 1374
TaqI TCGA 3 cut(s) 60, 509, 835
TasI AATT 3 cut(s) 540, 558, 1426
TatI WGTACW 1 cut(s) 1443
TfiI GAWTC 1 cut(s) 856
Tru1I TTAA 4 cut(s) 162, 543, 1101, 1269
Tru9I TTAA 4 cut(s) 162, 543, 1101, 1269
TscAI CASTG 3 cut(s) 123, 181, 1182
TseFI GTSAC 2 cut(s) 289, 367
TseI GCWGC 2 cut(s) 734, 1154
Tsp45I GTSAC 2 cut(s) 289, 367
TspDTI ATGAA 7 cut(s) 41, 285, 518, 796, 1013, 1187, 1392
TspRI CASTG 3 cut(s) 123, 181, 1182
Tth111I GACNNNGTC 1 cut(s) 1163
VneI GTGCAC 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 239
VspI ATTAAT 1 cut(s) 1101
XceI RCATGY 2 cut(s) 281, 361
XcmI CCANNNNNNNNNTGG 2 cut(s) 464, 838
XmaJI CCTAGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 1167
XmnI GAANNNNTTC 1 cut(s) 1252
XspI CTAG 2 cut(s) 438, 1415
ZrmI AGTACT 1 cut(s) 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.