Rh2CG071300

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000762
Physical Location & Seq
Forward (+)
5851793 .. 5855293
3501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG071300.1

Sequence Viewer

Length: 1299 bp
ATGGATGATACTGTTGCCCAAAAGATCATTGGTCACAAGAATAGTCCAAGGGGCATCCATTTTCGGCGTGCTGGACCACGTCAAAGAGTGTATTTTGAGGCTGAGGATGTTCATGCGTGTATTGTGACTTGTGGGGGTCTATGCCCTGGACTCAACACTGTGATTAGGGAGATCGTATGTGGTTTGCACCATATGTATGGTGTAAAGAAAGTTTCAGGGATAGTAGGAGGATACAAGGGTTTCTATTCTCGAAACATAATTCCTTTGACTCCAAAGAGTGTGAATGATATTCACAAACGCGGTGGAACCATCCTTGGGACATCAAGGGGTGGCCATGACAAATCAAAGATAGTTGATAGCATTAAAGATCGAGGAATCAATCAGGTTTATATAATTGGAGGAGATGGAACCCAGAAGGGAGCATCTGTAATATATGAGGAAATTAAAAGACGTGGTCTCAAAGTAGTAGTTGCTGGAATCCCCAAAACCATTGATAATGACATTCCGGTGATTGACAAATCCTTTGGCTTTGACTCTGCCGTTGAGGAGGCTCAACGTGCAATTAATGCAGCACATGTTGAATCTGAAAGCTTTGAGAATTGTATTGGTGTTGTGAAGGTGATGGGTCGGTATAGTGGGTTTATTGCTATGTATGCAACGCTTGCAAGCCGAGATGTAGACTGTTGCTTGATTCCAGAATCACCCTTTTATCTTGAGGGTCCTGGTGGACTGTTTGAATACATAGAGAAACGACTCAAAGAAAATGGGCACATGGTTATAGTCATTGCTGAAGGTGCTGGCCAGGAGCTTCTATCTGAAAGCATAAGCTCTATGGACAAACAGGATGCTTCAGGAAACAAGCTCCTCCAAGATGTTGGCCTATGGATTTCCCAGAAGATCAAGGATCACTTCTCGAAAAAAAGTAAAATGGCGATAAACCTCAAATATATAGATCCTACGTATATGATCCGAGCTATTCCGAGCAATGGTTCTGATAATGTGTACTGCACACTTCTTGCTCAAAGTGCTGTCCATGGAGCAATGGCAGGCTACACAGGATTTATAGTAGGTCCTGTCAATGGGAGACACTCTTATATACCCTTTCATAGAATAACCGAGGAGCAGCACCAGGTTGTGATAACAGACCGAATGTGGGCTCGGCTCCTATCTTCCACACACCAACCAAGTTTCCTGAGTACTAAAGATGTCATTGAGGACAAGAGAGATGAAGAAGTTCATCAGGAACATTGTTCAGATAACAACTTGGTGAACAAGGAAATCAGTTATACCTGTAATTGA

Protein Analysis

432

Amino Acids

47.67

Weight (kDa)

7.62

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 40 - 345 7.4e-64 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 300
AciI CCGC 1 cut(s) 300
AclWI GGATC 3 cut(s) 912, 947, 961
AcoI YGGCCR 2 cut(s) 331, 799
AcuI CTGAAG 2 cut(s) 810, 834
AfaI GTAC 2 cut(s) 1004, 1198
AfiI CCNNNNNNNGG 4 cut(s) 315, 986, 1079, 1153
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 2 cut(s) 581, 737
AjiI CACGTC 2 cut(s) 80, 452
AjnI CCWGG 4 cut(s) 145, 721, 801, 1128
AluBI AGCT 5 cut(s) 591, 808, 828, 862, 974
AluI AGCT 5 cut(s) 591, 808, 828, 862, 974
Alw26I GTCTC 2 cut(s) 461, 1078
AlwI GGATC 3 cut(s) 912, 947, 961
AoxI GGCC 3 cut(s) 331, 799, 877
ApeKI GCWGC 2 cut(s) 569, 1123
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 1233
AspS9I GGNCC 3 cut(s) 74, 719, 1070
AsuHPI GGTGA 4 cut(s) 520, 631, 693, 1279
AvaII GGWCC 3 cut(s) 74, 719, 1070
BaeGI GKGCMC 1 cut(s) 771
BaeI ACNNNNGTAYC 2 cut(s) 223, 256
BalI TGGCCA 2 cut(s) 333, 801
BanII GRGCYC 1 cut(s) 1159
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 581, 1135
BccI CCATC 3 cut(s) 317, 398, 616
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 4 cut(s) 147, 723, 803, 1130
BciVI GTATCC 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 461, 1078
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 2 cut(s) 570, 1124
BlsI GCNGC 2 cut(s) 571, 1125
BmcAI AGTACT 1 cut(s) 1198
Bme1390I CCNGG 4 cut(s) 147, 723, 803, 1130
Bme18I GGWCC 3 cut(s) 74, 719, 1070
BmgBI CACGTC 2 cut(s) 80, 452
BmgT120I GGNCC 3 cut(s) 74, 719, 1070
BmiI GGNNCC 4 cut(s) 307, 409, 720, 1163
BmrFI CCNGG 4 cut(s) 147, 723, 803, 1130
BmsI GCATC 3 cut(s) 63, 431, 835
Bpu10I CCTNAGC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 734
BsaAI YACGTR 1 cut(s) 960
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 5 cut(s) 47, 145, 313, 1033, 1116
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bsc4I CCNNNNNNNGG 4 cut(s) 315, 986, 1079, 1153
Bse3DI GCAATG 3 cut(s) 783, 991, 1047
BseBI CCWGG 4 cut(s) 147, 723, 803, 1130
BseDI CCNNGG 5 cut(s) 47, 145, 313, 1033, 1116
BseGI GGATG 5 cut(s) 10, 54, 112, 309, 850
BseLI CCNNNNNNNGG 4 cut(s) 315, 986, 1079, 1153
BseMI GCAATG 3 cut(s) 783, 991, 1047
BseMII CTCAG 2 cut(s) 93, 1184
BseRI GAGGAG 4 cut(s) 414, 560, 854, 1133
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 2 cut(s) 581, 1135
BsgI GTGCAG 1 cut(s) 991
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 333, 801, 879
BsiSI CCGG 1 cut(s) 506
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 4 cut(s) 315, 986, 1079, 1153
BsmAI GTCTC 2 cut(s) 461, 1078
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 3 cut(s) 333, 801, 879
Bso31I GGTCTC 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 771, 1159
Bsp143I GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 1 cut(s) 300
BspANI GGCC 3 cut(s) 333, 801, 879
BspCNI CTCAG 2 cut(s) 94, 1185
BspFNI CGCG 1 cut(s) 300
BspLI GGNNCC 4 cut(s) 307, 409, 720, 1163
BspPI GGATC 3 cut(s) 912, 947, 961
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 3 cut(s) 783, 991, 1047
BssECI CCNNGG 5 cut(s) 47, 145, 313, 1033, 1116
BssMI GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
BssT1I CCWWGG 3 cut(s) 47, 313, 1033
Bst2UI CCWGG 4 cut(s) 147, 723, 803, 1130
Bst4CI ACNGT 4 cut(s) 13, 160, 683, 732
BstAPI GCANNNNNTGC 2 cut(s) 566, 662
BstBAI YACGTR 1 cut(s) 960
BstC8I GCNNGC 5 cut(s) 69, 663, 667, 799, 1048
BstDEI CTNAG 2 cut(s) 102, 1193
BstDSI CCRYGG 1 cut(s) 1033
BstF5I GGATG 5 cut(s) 10, 54, 112, 309, 850
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 7 cut(s) 27, 174, 370, 900, 907, 955, 969
BstMAI GTCTC 2 cut(s) 461, 1078
BstMBI GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
BstMWI GCNNNNNNNGC 6 cut(s) 557, 566, 653, 662, 794, 1025
BstNI CCWGG 4 cut(s) 147, 723, 803, 1130
BstNSI RCATGY 1 cut(s) 578
BstSCI CCNGG 4 cut(s) 145, 721, 801, 1128
BstSLI GKGCMC 1 cut(s) 771
BstSNI TACGTA 1 cut(s) 960
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 581, 1135
BstX2I RGATCY 1 cut(s) 952
BstXI CCANNNNNNTGG 2 cut(s) 197, 875
BstYI RGATCY 1 cut(s) 952
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 3 cut(s) 333, 801, 879
BtgI CCRYGG 1 cut(s) 1033
BtrI CACGTC 2 cut(s) 80, 452
BtsCI GGATG 5 cut(s) 10, 54, 112, 309, 850
BtsIMutI CAGTG 1 cut(s) 156
Cac8I GCNNGC 5 cut(s) 69, 663, 667, 799, 1048
Cfr13I GGNCC 3 cut(s) 74, 719, 1070
CsiI ACCWGGT 1 cut(s) 1128
Csp6I GTAC 2 cut(s) 1003, 1197
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 5 cut(s) 113, 335, 575, 772, 1034
CviQI GTAC 2 cut(s) 1003, 1197
DdeI CTNAG 2 cut(s) 102, 1193
DpnI GATC 7 cut(s) 26, 173, 369, 899, 906, 954, 968
DpnII GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
EaeI YGGCCR 2 cut(s) 331, 799
Eco105I TACGTA 1 cut(s) 960
Eco130I CCWWGG 3 cut(s) 47, 313, 1033
Eco24I GRGCYC 1 cut(s) 1159
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 74, 719, 1070
Eco57I CTGAAG 2 cut(s) 810, 834
EcoO109I RGGNCCY 2 cut(s) 719, 1070
EcoRII CCWGG 4 cut(s) 145, 721, 801, 1128
EcoT14I CCWWGG 3 cut(s) 47, 313, 1033
EcoT38I GRGCYC 1 cut(s) 1159
ErhI CCWWGG 3 cut(s) 47, 313, 1033
FaeI CATG 5 cut(s) 116, 338, 578, 775, 1037
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FaqI GGGAC 1 cut(s) 331
FatI CATG 5 cut(s) 112, 334, 574, 771, 1033
FauNDI CATATG 1 cut(s) 192
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 570, 1124
FokI GGATG 5 cut(s) 17, 41, 119, 296, 857
FriOI GRGCYC 1 cut(s) 1159
Fsp4HI GCNGC 2 cut(s) 570, 1124
GluI GCNGC 2 cut(s) 570, 1124
HaeIII GGCC 3 cut(s) 333, 801, 879
HapII CCGG 1 cut(s) 506
Hin1II CATG 5 cut(s) 116, 338, 578, 775, 1037
HindIII AAGCTT 1 cut(s) 589
HinfI GANTC 9 cut(s) 150, 268, 375, 477, 533, 581, 691, 698, 753
HpaII CCGG 1 cut(s) 506
HphI GGTGA 4 cut(s) 520, 631, 693, 1279
Hpy166II GTNNAC 4 cut(s) 679, 728, 1003, 1270
Hpy188I TCNGA 6 cut(s) 586, 817, 971, 981, 994, 1255
Hpy188III TCNNGA 7 cut(s) 249, 695, 713, 852, 913, 1192, 1241
Hpy8I GTNNAC 4 cut(s) 679, 728, 1003, 1270
HpyAV CCTTC 3 cut(s) 409, 610, 785
HpyCH4III ACNGT 4 cut(s) 13, 160, 683, 732
HpyCH4IV ACGT 4 cut(s) 79, 451, 556, 959
HpyCH4V TGCA 6 cut(s) 187, 560, 569, 656, 665, 1008
HpyF10VI GCNNNNNNNGC 6 cut(s) 557, 566, 653, 662, 794, 1025
HpyF3I CTNAG 2 cut(s) 102, 1193
HpySE526I ACGT 4 cut(s) 79, 451, 556, 959
Hsp92II CATG 5 cut(s) 116, 338, 578, 775, 1037
Kzo9I GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
LmnI GCTCC 6 cut(s) 419, 805, 867, 1037, 1120, 1167
Lsp1109I GCAGC 2 cut(s) 581, 1135
LweI GCATC 3 cut(s) 63, 431, 835
MabI ACCWGGT 1 cut(s) 1128
MaeII ACGT 4 cut(s) 79, 451, 556, 959
MaeIII GTNAC 2 cut(s) 32, 124
MalI GATC 7 cut(s) 26, 173, 369, 899, 906, 954, 968
MboI GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
MboII GAAGA 3 cut(s) 907, 1161, 1241
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 2 cut(s) 771, 1159
MlsI TGGCCA 2 cut(s) 333, 801
MluCI AATT 6 cut(s) 258, 393, 441, 561, 598, 1294
MluNI TGGCCA 2 cut(s) 333, 801
MlyI GAGTC 4 cut(s) 144, 262, 527, 747
Mox20I TGGCCA 2 cut(s) 333, 801
MroXI GAANNNNTTC 1 cut(s) 1233
MscI TGGCCA 2 cut(s) 333, 801
MseI TTAA 3 cut(s) 363, 444, 564
MslI CAYNNNNRTG 2 cut(s) 195, 506
Msp20I TGGCCA 2 cut(s) 333, 801
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 4 cut(s) 147, 723, 803, 1130
MvaI CCWGG 4 cut(s) 147, 723, 803, 1130
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 6 cut(s) 557, 566, 653, 662, 794, 1025
NcoI CCATGG 1 cut(s) 1033
NdeI CATATG 1 cut(s) 192
NdeII GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
NlaIII CATG 5 cut(s) 116, 338, 578, 775, 1037
NlaIV GGNNCC 4 cut(s) 307, 409, 720, 1163
NmeAIII GCCGAG 2 cut(s) 695, 1138
NmuCI GTSAC 2 cut(s) 32, 124
NspI RCATGY 1 cut(s) 578
PciI ACATGT 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 1233
PfeI GAWTC 5 cut(s) 375, 477, 581, 691, 698
PflFI GACNNNGTC 2 cut(s) 78, 453
PkrI GCNGC 2 cut(s) 571, 1125
PleI GAGTC 4 cut(s) 144, 262, 527, 747
PpsI GAGTC 4 cut(s) 144, 262, 527, 747
Ppu21I YACGTR 1 cut(s) 960
PpuMI RGGWCCY 2 cut(s) 719, 1070
PscI ACATGT 1 cut(s) 574
PshBI ATTAAT 1 cut(s) 564
Psp5II RGGWCCY 2 cut(s) 719, 1070
Psp6I CCWGG 4 cut(s) 145, 721, 801, 1128
PspGI CCWGG 4 cut(s) 145, 721, 801, 1128
PspN4I GGNNCC 4 cut(s) 307, 409, 720, 1163
PspPI GGNCC 3 cut(s) 74, 719, 1070
PspPPI RGGWCCY 2 cut(s) 719, 1070
PsuI RGATCY 1 cut(s) 952
PsyI GACNNNGTC 2 cut(s) 78, 453
RsaI GTAC 2 cut(s) 1004, 1198
RsaNI GTAC 2 cut(s) 1003, 1197
RseI CAYNNNNRTG 2 cut(s) 195, 506
SaqAI TTAA 3 cut(s) 363, 444, 564
SatI GCNGC 2 cut(s) 570, 1124
Sau3AI GATC 7 cut(s) 24, 171, 367, 897, 904, 952, 966
Sau96I GGNCC 3 cut(s) 74, 719, 1070
ScaI AGTACT 1 cut(s) 1198
SchI GAGTC 4 cut(s) 144, 262, 527, 747
ScrFI CCNGG 4 cut(s) 147, 723, 803, 1130
SduI GDGCHC 2 cut(s) 771, 1159
SexAI ACCWGGT 1 cut(s) 1128
SfaNI GCATC 3 cut(s) 63, 431, 835
SinI GGWCC 3 cut(s) 74, 719, 1070
SmiMI CAYNNNNRTG 2 cut(s) 195, 506
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
SnaBI TACGTA 1 cut(s) 960
Sse9I AATT 6 cut(s) 258, 393, 441, 561, 598, 1294
SsiI CCGC 1 cut(s) 300
StyD4I CCNGG 4 cut(s) 145, 721, 801, 1128
StyI CCWWGG 3 cut(s) 47, 313, 1033
TaaI ACNGT 4 cut(s) 13, 160, 683, 732
TaiI ACGT 4 cut(s) 82, 454, 559, 962
TaqI TCGA 3 cut(s) 250, 370, 914
TaqII GACCGA 1 cut(s) 1161
TasI AATT 6 cut(s) 258, 393, 441, 561, 598, 1294
TatI WGTACW 2 cut(s) 1002, 1196
TfiI GAWTC 5 cut(s) 375, 477, 581, 691, 698
Tru1I TTAA 3 cut(s) 363, 444, 564
Tru9I TTAA 3 cut(s) 363, 444, 564
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 2 cut(s) 32, 124
TseI GCWGC 2 cut(s) 569, 1123
Tsp45I GTSAC 2 cut(s) 32, 124
TspDTI ATGAA 4 cut(s) 101, 1094, 1226, 1242
TspRI CASTG 1 cut(s) 163
Tth111I GACNNNGTC 2 cut(s) 78, 453
VpaK11BI GGWCC 3 cut(s) 74, 719, 1070
VspI ATTAAT 1 cut(s) 564
XceI RCATGY 1 cut(s) 578
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 678
XmnI GAANNNNTTC 1 cut(s) 1233
ZrmI AGTACT 1 cut(s) 1198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.