Rorug06G0352400

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000762
Physical Location & Seq
Forward (+)
50155238 .. 50161590
6353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0352400.1

Sequence Viewer

Length: 3081 bp
ATGGCGTCTCCGACGTCCCCAACGTCGTCGTGCAGCTCTGCGCACTCGTCGACATTTGCATCGTCCAAATTCTCGGACCTTCCGGTGATGGCTCTCCGGGAGAAAATCGTCGAGAAAATCCTCGAGAATCGCGTCACTCTCATCGTCGGCGAGACCGGTTGCGGGAAAAGCTCGCAAATACCGCAGTTTCTTCTGGAAGCGAATGTGAAGCCAATTCTATGTACACAGCCTAGGAGGTTTGCGGTAGTAGCTGTTGCAAAAATGGTTGCACAAGCTCGTAATAGTGAGCTTGGTGGAGAGGTTGGATATCACATAGGCCACTCAAAGCACTTGTCACCAAGATCAGTGATTGCTTTTAAAACTGCTGGAGTTTTACTGGATGAAATGCGGGACAAGGGGATGCATGCGCTTGATTACAAGGTCATTGTTCTCGATGAAGTGCATGAAAGATCTGTGGAGTCTGATCTTGTTCTTGTTTGTGTGAAGCAGTTTATGATGAAGAACAATAACTTGAGGGTGGTGTTGATGTCTGCAACTGCTGATATTAATAGGTACAGGGATTACTTTAAGGATCTTGGCAGGGATGAACGAGTGGAAGTGCTTGCAATCTCTACCTCTGGCCAGAAAACCATTTTTCAGAAAAGGGTTTCTTATCTTGAAGAGGTGACTGATCTTCTCAACATCAATTCGGAGTCGCTTTCTTTGACATATTGTTCAGGACCAAGTCCTTCATTGGCCAAAGCTGATATGAAGCCTGAAGTGCACAATATTATTCATCAATTGGTGTTGCATATTCACGAGCAAGAGCCCGACATTGAAAAGAGCATTTTGATTTTTCTTCCAACATACTATGCCCTGGAGCAGCAATGGTTCCTTCTGAAGCCTCTTAGTTCATCTTTTAAAGTTCACATTTTACATAGCAGCATTGATACTGAACAAGCTCTCATGACCATGAAGATCTGGAAATCCCATCGTAAAGTGATACTGGCCACAAACATTGCTGAATCATCAGTAACAATTCCCAAGGTGGCTTACGTTATTGATTCATGCCGATCTTTACAAGTCTTCTGGCACACTTATGAGAAAAGGGAATGTGCAAGCCTTGTTTGGGTTTCTAAGTCTCAGGCTGATCAGCGTAAAGGGAGAACTGGTCGAACATGTGATGGCCAGATTTATCGGTTGGTTACTCGATCATTTTATGGCCAGCTTGAGGATTACGAAGGTCCCTCTATACTGAGGTTATCATTGAGGATGCAAGTGCTTCACATTTGCTGCTCTGATTCCAAAGCCATTAATGATCCCAAGGCCTTGTTGCAGAAGGCCTTAGATCCACCACCTCCTGATGTTATTGAAGACGCGTTGAATTTGCTGGTTCATATGCAAGCATTGGAAAGAACACCTCCAAGGGGTCGGTATGAGCCTACATTTTATGGGCGATTGCTGGCCAGTTTCTCATTGTCTTTTGATGCCTCTGTGCTTGTACTTAAGTTTGGAGATGTTGGAATGCTGCGTGAAGGCATTCTTCTGGGCATATTAATGGATACACAACCTCTGCCTATTCTTCGTCCTTTTGGAGATGAACTTCTGAGTTCACAATATGCTGATAGCTACTTTAGTGGAGATGATTACAGTACTGGCCTAACGGGAAAGAAGGAAACAGCATTTATGGGAAACTTGTGCGCATTTCAATTTTGGCAACGTGTTTTCAAGGATAAACACCGTGTTGAGAATTTGAAGCAACTTCTTCAGTTTGATGTGATGAAAGCTACTATATTGGAACTTCCCAAGATGGAAGACTGGTGCTCTTTCCATAATCTTGTGCACTCATCACTGAATCATGTCTCTGAAATATATGAAGATATTCTTCATTCAGTGCACCGGTTTCGCCCCAAGTTTTTGTCCACATCTAATGGCTTACCATCCTACTATGATCCGTATGAATATGAGCATACATGTATTCTTACATGTCAACAACCAAATGGAGATACAGATGCTCTTGCTACGGACGATAGGCATCTTCAGCCATCTGGTGAAACAACAAAATGTTTTGCTGTACCTTTTGTTGCTTCAGATCACTTCCGACAGAATGATGTGGCTAAAAAGTTGGCAATGATAGTGAAACAGATAAGAGTACCGAATACCGAAGATGCATCGAGTAATCAGGGTTTAAATGTTGGTGACGGTTATCATGTCGATGGGGAGGCTCCTGTTTGTATTTATTTTATCAATGGGTCCTGCAAATGGGGCAGTGAATGCAGGTTTTCTCATTCACTCAAAGGACAGAGAACTCAGTGCAAATTTTTCTTTACTCTAAAGGGTTGTCGAAACGGAGAGTCATGCATGTTTTCTCATAATGAGAGTCCATCACTAACATCATCCAGCTCATCTAGCTCATCCAGCTCAAACTTTTGCCTGCCAGAAGATGGCAAAGCTACAGCTCTGTCCCTATTAAATCTGTTTTCCTTTTCTTCAGATGGATACCTTCTTTTACTGGATGACACCAATCTACATTTTACCTCAAAGTTAGCTAGCTTTTATGAACCATCCAAAATAATCTCCACAACATGTTTGTCAGACACATCCATATTTGACCCTTCATTGACGGGTATCCAAATTTTGTGGGGGCTTCATCACCCATATCAGACCATCATTTCCAAAGGAGTGAACCAGATCCCCTGGAAAGAAGTTAAGTACGTGCTCTGGTTTCCTAATTTTGAAAGCTACAGTGAGAATTTGGAAATACAGAAAACTCTCTTGCAGAGTTTCTTCGAGTATCTTGCCATCCGAATGTTGGGTGATGCCCTGGAAATGCGAGTCATTCTCACCATGAACAATATCAGGTTTTCACAGCTGCAGGCGGAGAAGTTTGGTAGGGATAGCTTTTTCTTCCTTAAAGAGTCATTCCCATTTGATGAGAACAGCTTCGGAGAGTTGCCAGACAAGCTCAGCACAAAGAAGCCAATGATGGCATCAAGGCCAACTTCCTATGTTTTCGACCTACACCCACCTTCAGACTTTCAGTTTGGTAACTATGCGGCCGTGCTTCAAAAATCTCTTCGCGGTGTCCAGGAACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

1026

Amino Acids

115.85

Weight (kDa)

6.43

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 34 - 181 3e-07 DEAD/DEAH box helicase
Helicase_C PF00271 269 - 386 2.2e-12 Helicase conserved C-terminal domain
zf-CCCH_4 PF18044 736 - 756 2.2e-06 CCCH-type zinc finger
zf_CCCH_4 PF18345 738 - 756 5.3e-06 Zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 17
Acc16I TGCGCA 2 cut(s) 42, 1681
Acc36I ACCTGC 1 cut(s) 2247
AccB7I CCANNNNNTGG 1 cut(s) 2423
AccI GTMKAC 1 cut(s) 50
AccII CGCG 3 cut(s) 132, 1358, 3060
AciI CCGC 7 cut(s) 162, 182, 242, 388, 2858, 3035, 3060
AclWI GGATC 5 cut(s) 579, 1292, 1322, 1925, 2665
AcoI YGGCCR 7 cut(s) 619, 735, 987, 1165, 1201, 1443, 3036
AcsI RAATTY 6 cut(s) 68, 1363, 1729, 2297, 2613, 2731
AcuI CTGAAG 7 cut(s) 777, 899, 1730, 2003, 2052, 2454, 2994
AcyI GRCGYC 2 cut(s) 5, 14
AfaI GTAC 7 cut(s) 223, 554, 1482, 1633, 2055, 2133, 2693
AfiI CCNNNNNNNGG 7 cut(s) 162, 1108, 1210, 1406, 2241, 2423, 2791
AflII CTTAAG 1 cut(s) 1484
AflIII ACRYGT 6 cut(s) 1157, 1356, 1699, 1952, 1964, 2564
AgeI ACCGGT 2 cut(s) 155, 1878
AgsI TTSAA 9 cut(s) 659, 818, 1352, 1363, 1688, 1708, 1735, 2717, 3047
AjnI CCWGG 4 cut(s) 855, 2675, 2802, 3066
Alw21I GWGCWC 5 cut(s) 765, 1805, 1824, 1878, 2700
Alw26I GTCTC 4 cut(s) 12, 146, 1125, 1846
Alw44I GTGCAC 3 cut(s) 761, 1820, 1874
AlwI GGATC 5 cut(s) 579, 1292, 1322, 1925, 2665
Ama87I CYCGRG 1 cut(s) 122
ApaLI GTGCAC 3 cut(s) 761, 1820, 1874
ApeKI GCWGC 6 cut(s) 33, 862, 921, 1272, 1507, 2851
ApoI RAATTY 6 cut(s) 68, 1363, 1729, 2297, 2613, 2731
ArsI GACNNNNNNTTYG 4 cut(s) 317, 349, 3005, 3037
AseI ATTAAT 3 cut(s) 546, 1293, 1535
AsiGI ACCGGT 2 cut(s) 155, 1878
Asp700I GAANNNNTTC 3 cut(s) 1518, 1860, 2921
AspA2I CCTAGG 1 cut(s) 230
AspLEI GCGC 3 cut(s) 43, 409, 1682
AspS9I GGNCC 4 cut(s) 76, 719, 1223, 2232
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 8 cut(s) 97, 327, 676, 2042, 2189, 2624, 2807, 2815
AsuNHI GCTAGC 1 cut(s) 2528
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 4 cut(s) 76, 719, 1223, 2232
AvrII CCTAGG 1 cut(s) 230
BaeGI GKGCMC 3 cut(s) 765, 1824, 1878
BalI TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
BanII GRGCYC 1 cut(s) 810
BauI CACGAG 1 cut(s) 797
BbsI GAAGAC 3 cut(s) 1057, 1359, 1800
Bbv12I GWGCWC 5 cut(s) 765, 1805, 1824, 1878, 2700
BbvI GCAGC 6 cut(s) 45, 874, 933, 1259, 1494, 2838
BceAI ACGGC 1 cut(s) 3023
BcgI CGANNNNNNTGC 4 cut(s) 1865, 1899, 2759, 2793
BciT130I CCWGG 4 cut(s) 857, 2677, 2804, 3068
BciVI GTATCC 3 cut(s) 1534, 2471, 2618
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 12, 146, 1125, 1846
BfaI CTAG 3 cut(s) 231, 2388, 2529
BfmI CTRYAG 3 cut(s) 2433, 2722, 2852
BfrI CTTAAG 1 cut(s) 1484
BfuAI ACCTGC 1 cut(s) 2247
BfuI GTATCC 3 cut(s) 1534, 2471, 2618
BglII AGATCT 2 cut(s) 449, 957
BisI GCNGC 7 cut(s) 34, 863, 922, 1273, 1508, 2852, 3036
BlnI CCTAGG 1 cut(s) 230
BlpI GCTNAGC 1 cut(s) 2945
BlsI GCNGC 7 cut(s) 35, 864, 923, 1274, 1509, 2853, 3037
BmcAI AGTACT 1 cut(s) 1633
Bme1390I CCNGG 5 cut(s) 98, 857, 2677, 2804, 3068
Bme18I GGWCC 4 cut(s) 76, 719, 1223, 2232
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 4 cut(s) 76, 719, 1223, 2232
BmiI GGNNCC 4 cut(s) 872, 1225, 2205, 2233
BmrFI CCNGG 5 cut(s) 98, 857, 2677, 2804, 3068
BmtI GCTAGC 1 cut(s) 2532
BpiI GAAGAC 3 cut(s) 1057, 1359, 1800
BplI GAGNNNNNCTC 2 cut(s) 2805, 2837
BpmI CTGGAG 2 cut(s) 387, 878
Bpu1102I GCTNAGC 1 cut(s) 2945
BpuEI CTTGAG 2 cut(s) 532, 1229
BpuMI CCSGG 1 cut(s) 98
BsaAI YACGTR 1 cut(s) 2695
BsaBI GATNNNNATC 1 cut(s) 2013
BsaHI GRCGYC 2 cut(s) 5, 14
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 7 cut(s) 230, 855, 1023, 1302, 1403, 2675, 2802
BsaWI WCCGGW 3 cut(s) 82, 155, 1878
BsaXI ACNNNNNCTCC 2 cut(s) 1135, 1165
Bsc4I CCNNNNNNNGG 7 cut(s) 162, 1108, 1210, 1406, 2241, 2423, 2791
Bse118I RCCGGY 2 cut(s) 155, 1878
Bse1I ACTGG 7 cut(s) 381, 990, 1153, 1446, 1639, 1802, 2496
Bse3DI GCAATG 3 cut(s) 872, 996, 2115
Bse8I GATNNNNATC 1 cut(s) 2013
BseBI CCWGG 4 cut(s) 857, 2677, 2804, 3068
BseDI CCNNGG 7 cut(s) 230, 855, 1023, 1302, 1403, 2675, 2802
BseJI GATNNNNATC 1 cut(s) 2013
BseLI CCNNNNNNNGG 7 cut(s) 162, 1108, 1210, 1406, 2241, 2423, 2791
BseMI GCAATG 3 cut(s) 872, 996, 2115
BseMII CTCAG 5 cut(s) 1136, 1226, 1577, 2303, 2959
BseNI ACTGG 7 cut(s) 381, 990, 1153, 1446, 1639, 1802, 2496
BseSI GKGCMC 3 cut(s) 765, 1824, 1878
BseX3I CGGCCG 1 cut(s) 3036
BseXI GCAGC 6 cut(s) 45, 874, 933, 1259, 1494, 2838
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 3 cut(s) 132, 1358, 3060
Bsh1285I CGRYCG 1 cut(s) 3039
BshTI ACCGGT 2 cut(s) 155, 1878
BsiEI CGRYCG 1 cut(s) 3039
BsiHKAI GWGCWC 5 cut(s) 765, 1805, 1824, 1878, 2700
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 4 cut(s) 83, 97, 156, 1879
BslFI GGGAC 3 cut(s) 404, 1209, 2428
BslI CCNNNNNNNGG 7 cut(s) 162, 1108, 1210, 1406, 2241, 2423, 2791
BsmAI GTCTC 4 cut(s) 12, 146, 1125, 1846
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 404, 1209, 2428
BsmI GAATGC 3 cut(s) 1509, 1518, 2258
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 6 cut(s) 765, 810, 1805, 1824, 1878, 2700
Bsp1407I TGTACA 1 cut(s) 221
Bsp1720I GCTNAGC 1 cut(s) 2945
BspACI CCGC 7 cut(s) 162, 182, 242, 388, 2858, 3035, 3060
BspCNI CTCAG 5 cut(s) 1135, 1227, 1578, 2302, 2958
BspFNI CGCG 3 cut(s) 132, 1358, 3060
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 4 cut(s) 872, 1225, 2205, 2233
BspMAI CTGCAG 1 cut(s) 2856
BspMI ACCTGC 1 cut(s) 2247
BspOI GCTAGC 1 cut(s) 2532
BspPI GGATC 5 cut(s) 579, 1292, 1322, 1925, 2665
BspTI CTTAAG 1 cut(s) 1484
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 3 cut(s) 872, 996, 2115
BsrFI RCCGGY 2 cut(s) 155, 1878
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 7 cut(s) 381, 990, 1153, 1446, 1639, 1802, 2496
BssAI RCCGGY 2 cut(s) 155, 1878
BssECI CCNNGG 7 cut(s) 230, 855, 1023, 1302, 1403, 2675, 2802
BssNI GRCGYC 2 cut(s) 5, 14
BssSI CACGAG 1 cut(s) 797
BssT1I CCWWGG 4 cut(s) 230, 1023, 1302, 1403
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 4 cut(s) 857, 2677, 2804, 3068
Bst4CI ACNGT 4 cut(s) 1631, 1721, 2183, 2726
Bst6I CTCTTC 2 cut(s) 654, 3060
BstACI GRCGYC 2 cut(s) 5, 14
BstAFI CTTAAG 1 cut(s) 1484
BstAPI GCANNNNNTGC 1 cut(s) 2253
BstAUI TGTACA 1 cut(s) 221
BstBAI YACGTR 1 cut(s) 2695
BstDEI CTNAG 8 cut(s) 887, 1116, 1122, 1235, 1324, 1586, 2289, 2945
BstFNI CGCG 3 cut(s) 132, 1358, 3060
BstHHI GCGC 3 cut(s) 43, 409, 1682
BstMAI GTCTC 4 cut(s) 12, 146, 1125, 1846
BstMCI CGRYCG 1 cut(s) 3039
BstNI CCWGG 4 cut(s) 857, 2677, 2804, 3068
BstNSI RCATGY 6 cut(s) 407, 1161, 1956, 1968, 2344, 2568
BstSCI CCNGG 5 cut(s) 96, 855, 2675, 2802, 3066
BstSFI CTRYAG 3 cut(s) 2433, 2722, 2852
BstSLI GKGCMC 3 cut(s) 765, 1824, 1878
BstUI CGCG 3 cut(s) 132, 1358, 3060
BstV1I GCAGC 6 cut(s) 45, 874, 933, 1259, 1494, 2838
BstV2I GAAGAC 3 cut(s) 1057, 1359, 1800
BstX2I RGATCY 5 cut(s) 449, 571, 957, 1327, 2670
BstYI RGATCY 5 cut(s) 449, 571, 957, 1327, 2670
BstZI CGGCCG 1 cut(s) 3036
BsuI GTATCC 3 cut(s) 1534, 2471, 2618
BtsI GCAGTG 1 cut(s) 2254
BtsIMutI CAGTG 6 cut(s) 351, 1829, 1878, 2254, 2297, 2731
BveI ACCTGC 1 cut(s) 2247
CciI TCATGA 1 cut(s) 945
CfoI GCGC 3 cut(s) 43, 409, 1682
Cfr10I RCCGGY 2 cut(s) 155, 1878
Cfr13I GGNCC 4 cut(s) 76, 719, 1223, 2232
CseI GACGC 2 cut(s) 121, 1364
Csp6I GTAC 7 cut(s) 222, 553, 1481, 1632, 2054, 2132, 2692
CspAI ACCGGT 2 cut(s) 155, 1878
CspCI CAANNNNNGTGG 4 cut(s) 979, 1014, 2600, 2635
CviQI GTAC 7 cut(s) 222, 553, 1481, 1632, 2054, 2132, 2692
DdeI CTNAG 8 cut(s) 887, 1116, 1122, 1235, 1324, 1586, 2289, 2945
DraI TTTAAA 3 cut(s) 358, 901, 2169
EaeI YGGCCR 7 cut(s) 619, 735, 987, 1165, 1201, 1443, 3036
EagI CGGCCG 1 cut(s) 3036
Eam1104I CTCTTC 2 cut(s) 654, 3060
EarI CTCTTC 2 cut(s) 654, 3060
EciI GGCGGA 1 cut(s) 2873
EclXI CGGCCG 1 cut(s) 3036
Eco130I CCWWGG 4 cut(s) 230, 1023, 1302, 1403
Eco147I AGGCCT 2 cut(s) 1307, 1322
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 308
Eco47I GGWCC 4 cut(s) 76, 719, 1223, 2232
Eco52I CGGCCG 1 cut(s) 3036
Eco57I CTGAAG 7 cut(s) 777, 899, 1730, 2003, 2052, 2454, 2994
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 2 cut(s) 1223, 2232
EcoRII CCWGG 4 cut(s) 855, 2675, 2802, 3066
EcoRV GATATC 1 cut(s) 308
EcoT14I CCWWGG 4 cut(s) 230, 1023, 1302, 1403
EcoT22I ATGCAT 3 cut(s) 405, 2152, 2342
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 4 cut(s) 230, 1023, 1302, 1403
Esp3I CGTCTC 1 cut(s) 12
FalI AAGNNNNNCTT 8 cut(s) 634, 666, 1506, 1538, 1848, 1880, 2965, 2997
FaqI GGGAC 3 cut(s) 404, 1209, 2428
FauI CCCGC 2 cut(s) 155, 381
FauNDI CATATG 1 cut(s) 1377
FbaI TGATCA 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 7 cut(s) 34, 863, 922, 1273, 1508, 2852, 3036
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 7 cut(s) 34, 863, 922, 1273, 1508, 2852, 3036
FspAI RTGCGCAY 1 cut(s) 1681
FspBI CTAG 3 cut(s) 231, 2388, 2529
FspI TGCGCA 2 cut(s) 42, 1681
GlaI GCGC 3 cut(s) 42, 408, 1681
GluI GCNGC 7 cut(s) 34, 863, 922, 1273, 1508, 2852, 3036
GsuI CTGGAG 2 cut(s) 387, 878
HapII CCGG 4 cut(s) 83, 97, 156, 1879
HgaI GACGC 2 cut(s) 121, 1364
HhaI GCGC 3 cut(s) 43, 409, 1682
Hin1I GRCGYC 2 cut(s) 5, 14
Hin6I GCGC 3 cut(s) 41, 407, 1680
HinP1I GCGC 3 cut(s) 41, 407, 1680
HincII GTYRAC 2 cut(s) 51, 1970
HindII GTYRAC 2 cut(s) 51, 1970
HpaII CCGG 4 cut(s) 83, 97, 156, 1879
HphI GGTGA 8 cut(s) 97, 327, 676, 2042, 2189, 2624, 2807, 2815
Hpy99I CGWCG 6 cut(s) 16, 28, 31, 52, 113, 149
HpyCH4III ACNGT 4 cut(s) 1631, 1721, 2183, 2726
HpyCH4IV ACGT 5 cut(s) 14, 23, 1035, 1699, 2694
HpyF3I CTNAG 8 cut(s) 887, 1116, 1122, 1235, 1324, 1586, 2289, 2945
HpySE526I ACGT 5 cut(s) 14, 23, 1035, 1699, 2694
Hsp92I GRCGYC 2 cut(s) 5, 14
HspAI GCGC 3 cut(s) 41, 407, 1680
Ksp22I TGATCA 1 cut(s) 1129
LmnI GCTCC 2 cut(s) 859, 2209
Lsp1109I GCAGC 6 cut(s) 45, 874, 933, 1259, 1494, 2838
MaeI CTAG 3 cut(s) 231, 2388, 2529
MaeII ACGT 5 cut(s) 14, 23, 1035, 1699, 2694
MaeIII GTNAC 7 cut(s) 133, 333, 664, 1012, 1183, 2177, 3026
MfeI CAATTG 1 cut(s) 779
MflI RGATCY 5 cut(s) 449, 571, 957, 1327, 2670
MhlI GDGCHC 6 cut(s) 765, 810, 1805, 1824, 1878, 2700
MlsI TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
MluI ACGCGT 1 cut(s) 1356
MluNI TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
MlyI GAGTC 6 cut(s) 467, 701, 2342, 2368, 2823, 2906
MmeI TCCRAC 5 cut(s) 35, 283, 866, 1480, 2104
Mox20I TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
Mph1103I ATGCAT 3 cut(s) 405, 2152, 2342
MroXI GAANNNNTTC 3 cut(s) 1518, 1860, 2921
MscI TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
MslI CAYNNNNRTG 5 cut(s) 950, 1077, 1535, 2193, 2786
Msp20I TGGCCA 6 cut(s) 621, 737, 989, 1167, 1203, 1445
MspA1I CMGCKG 1 cut(s) 2851
MspCI CTTAAG 1 cut(s) 1484
MspI CCGG 4 cut(s) 83, 97, 156, 1879
MspR9I CCNGG 5 cut(s) 98, 857, 2677, 2804, 3068
MunI CAATTG 1 cut(s) 779
Mva1269I GAATGC 3 cut(s) 1509, 1518, 2258
MvaI CCWGG 4 cut(s) 857, 2677, 2804, 3068
MvnI CGCG 3 cut(s) 132, 1358, 3060
NciI CCSGG 1 cut(s) 98
NdeI CATATG 1 cut(s) 1377
NheI GCTAGC 1 cut(s) 2528
NlaIV GGNNCC 4 cut(s) 872, 1225, 2205, 2233
NmuCI GTSAC 4 cut(s) 133, 333, 664, 2177
NsbI TGCGCA 2 cut(s) 42, 1681
NsiI ATGCAT 3 cut(s) 405, 2152, 2342
NspI RCATGY 6 cut(s) 407, 1161, 1956, 1968, 2344, 2568
PaeI GCATGC 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 945
PceI AGGCCT 2 cut(s) 1307, 1322
PciI ACATGT 4 cut(s) 1157, 1952, 1964, 2564
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 129
PctI GAATGC 3 cut(s) 1509, 1518, 2258
PdmI GAANNNNTTC 3 cut(s) 1518, 1860, 2921
PfeI GAWTC 5 cut(s) 127, 1004, 1043, 1280, 1834
PflFI GACNNNGTC 1 cut(s) 723
PflMI CCANNNNNTGG 1 cut(s) 2423
PfoI TCCNGGA 2 cut(s) 96, 3066
PinAI ACCGGT 2 cut(s) 155, 1878
PkrI GCNGC 7 cut(s) 35, 864, 923, 1274, 1509, 2853, 3037
PleI GAGTC 6 cut(s) 466, 700, 2341, 2367, 2822, 2905
PpsI GAGTC 6 cut(s) 466, 700, 2341, 2367, 2822, 2905
Ppu21I YACGTR 1 cut(s) 2695
PpuMI RGGWCCY 2 cut(s) 1223, 2232
PscI ACATGT 4 cut(s) 1157, 1952, 1964, 2564
PshBI ATTAAT 3 cut(s) 546, 1293, 1535
Psp5II RGGWCCY 2 cut(s) 1223, 2232
Psp6I CCWGG 4 cut(s) 855, 2675, 2802, 3066
PspGI CCWGG 4 cut(s) 855, 2675, 2802, 3066
PspN4I GGNNCC 4 cut(s) 872, 1225, 2205, 2233
PspPI GGNCC 4 cut(s) 76, 719, 1223, 2232
PspPPI RGGWCCY 2 cut(s) 1223, 2232
PstI CTGCAG 1 cut(s) 2856
PsuI RGATCY 5 cut(s) 449, 571, 957, 1327, 2670
PsyI GACNNNGTC 1 cut(s) 723
PvuII CAGCTG 1 cut(s) 2851
RsaI GTAC 7 cut(s) 223, 554, 1482, 1633, 2055, 2133, 2693
RsaNI GTAC 7 cut(s) 222, 553, 1481, 1632, 2054, 2132, 2692
RseI CAYNNNNRTG 5 cut(s) 950, 1077, 1535, 2193, 2786
SalI GTCGAC 1 cut(s) 49
SatI GCNGC 7 cut(s) 34, 863, 922, 1273, 1508, 2852, 3036
Sau96I GGNCC 4 cut(s) 76, 719, 1223, 2232
ScaI AGTACT 1 cut(s) 1633
SchI GAGTC 6 cut(s) 467, 701, 2342, 2368, 2823, 2906
ScrFI CCNGG 5 cut(s) 98, 857, 2677, 2804, 3068
SduI GDGCHC 6 cut(s) 765, 810, 1805, 1824, 1878, 2700
SfcI CTRYAG 3 cut(s) 2433, 2722, 2852
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 4 cut(s) 76, 719, 1223, 2232
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 5 cut(s) 950, 1077, 1535, 2193, 2786
SmlI CTYRAG 4 cut(s) 122, 511, 1208, 1484
SmoI CTYRAG 4 cut(s) 122, 511, 1208, 1484
SphI GCATGC 1 cut(s) 407
SseBI AGGCCT 2 cut(s) 1307, 1322
SsiI CCGC 7 cut(s) 162, 182, 242, 388, 2858, 3035, 3060
SspI AATATT 1 cut(s) 769
SspMI CTAG 3 cut(s) 231, 2388, 2529
StuI AGGCCT 2 cut(s) 1307, 1322
StyD4I CCNGG 5 cut(s) 96, 855, 2675, 2802, 3066
StyI CCWWGG 4 cut(s) 230, 1023, 1302, 1403
TaaI ACNGT 4 cut(s) 1631, 1721, 2183, 2726
TaiI ACGT 5 cut(s) 17, 26, 1038, 1702, 2697
TatI WGTACW 3 cut(s) 221, 1480, 1631
TauI GCSGC 1 cut(s) 3038
TfiI GAWTC 5 cut(s) 127, 1004, 1043, 1280, 1834
TscAI CASTG 6 cut(s) 351, 1836, 1878, 2254, 2297, 2731
TseFI GTSAC 4 cut(s) 133, 333, 664, 2177
TseI GCWGC 6 cut(s) 33, 862, 921, 1272, 1507, 2851
Tsp45I GTSAC 4 cut(s) 133, 333, 664, 2177
TspGWI ACGGA 3 cut(s) 1923, 2018, 2343
TspRI CASTG 6 cut(s) 351, 1836, 1878, 2254, 2297, 2731
Tth111I GACNNNGTC 1 cut(s) 723
Van91I CCANNNNNTGG 1 cut(s) 2423
Vha464I CTTAAG 1 cut(s) 1484
VneI GTGCAC 3 cut(s) 761, 1820, 1874
VpaK11BI GGWCC 4 cut(s) 76, 719, 1223, 2232
VspI ATTAAT 3 cut(s) 546, 1293, 1535
XapI RAATTY 6 cut(s) 68, 1363, 1729, 2297, 2613, 2731
XceI RCATGY 6 cut(s) 407, 1161, 1956, 1968, 2344, 2568
XcmI CCANNNNNNNNNTGG 1 cut(s) 2788
XhoI CTCGAG 1 cut(s) 122
XmaJI CCTAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 3 cut(s) 1518, 1860, 2921
XspI CTAG 3 cut(s) 231, 2388, 2529
ZraI GACGTC 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 1633
Zsp2I ATGCAT 3 cut(s) 405, 2152, 2342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.