Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 712, 1071 |
| AciI | CCGC | 2 cut(s) | 5, 266 |
| AclWI | GGATC | 5 cut(s) | 882, 974, 981, 1016, 1258 |
| AcsI | RAATTY | 1 cut(s) | 642 |
| AcuI | CTGAAG | 3 cut(s) | 41, 717, 903 |
| AcyI | GRCGYC | 2 cut(s) | 45, 613 |
| AfaI | GTAC | 4 cut(s) | 472, 675, 809, 1349 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1148, 1198, 1294 |
| AflIII | ACRYGT | 1 cut(s) | 357 |
| AgeI | ACCGGT | 1 cut(s) | 670 |
| AgsI | TTSAA | 5 cut(s) | 785, 815, 938, 1242, 1280 |
| AjnI | CCWGG | 3 cut(s) | 310, 371, 1048 |
| AleI | CACNNNNGTG | 1 cut(s) | 462 |
| AluBI | AGCT | 4 cut(s) | 891, 931, 1061, 1257 |
| AluI | AGCT | 4 cut(s) | 891, 931, 1061, 1257 |
| AlwI | GGATC | 5 cut(s) | 882, 974, 981, 1016, 1258 |
| AoxI | GGCC | 3 cut(s) | 496, 619, 1139 |
| ApeKI | GCWGC | 1 cut(s) | 1058 |
| ApoI | RAATTY | 1 cut(s) | 642 |
| AseI | ATTAAT | 1 cut(s) | 1005 |
| AsiGI | ACCGGT | 1 cut(s) | 670 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1156 |
| AspA2I | CCTAGG | 1 cut(s) | 437 |
| AspLEI | GCGC | 1 cut(s) | 1191 |
| AspS9I | GGNCC | 2 cut(s) | 239, 1139 |
| AsuHPI | GGTGA | 4 cut(s) | 26, 337, 361, 578 |
| AvaII | GGWCC | 1 cut(s) | 239 |
| AvrII | CCTAGG | 1 cut(s) | 437 |
| BaeGI | GKGCMC | 1 cut(s) | 1228 |
| BanII | GRGCYC | 1 cut(s) | 318 |
| BauI | CACGAG | 1 cut(s) | 489 |
| BbsI | GAAGAC | 1 cut(s) | 1159 |
| BbvCI | CCTCAGC | 1 cut(s) | 858 |
| BbvI | GCAGC | 1 cut(s) | 1070 |
| BccI | CCATC | 2 cut(s) | 383, 563 |
| BceAI | ACGGC | 2 cut(s) | 600, 1082 |
| BciT130I | CCWGG | 3 cut(s) | 312, 373, 1050 |
| BclI | TGATCA | 2 cut(s) | 327, 1035 |
| BfaI | CTAG | 3 cut(s) | 438, 1319, 1351 |
| BfmI | CTRYAG | 1 cut(s) | 141 |
| BfoI | RGCGCY | 1 cut(s) | 1192 |
| BisI | GCNGC | 1 cut(s) | 1059 |
| BlnI | CCTAGG | 1 cut(s) | 437 |
| BlsI | GCNGC | 1 cut(s) | 1060 |
| BmcAI | AGTACT | 1 cut(s) | 1349 |
| Bme1390I | CCNGG | 3 cut(s) | 312, 373, 1050 |
| Bme18I | GGWCC | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 2 cut(s) | 239, 1139 |
| BmiI | GGNNCC | 1 cut(s) | 586 |
| BmrFI | CCNGG | 3 cut(s) | 312, 373, 1050 |
| BmsI | GCATC | 1 cut(s) | 904 |
| BpiI | GAAGAC | 1 cut(s) | 1159 |
| Bpu10I | CCTNAGC | 1 cut(s) | 858 |
| BsaBI | GATNNNNATC | 1 cut(s) | 29 |
| BsaHI | GRCGYC | 2 cut(s) | 45, 613 |
| BsaJI | CCNNGG | 6 cut(s) | 310, 311, 372, 437, 478, 616 |
| BsaWI | WCCGGW | 2 cut(s) | 11, 670 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 576, 606 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1148, 1198, 1294 |
| Bse118I | RCCGGY | 2 cut(s) | 47, 670 |
| Bse3DI | GCAATG | 1 cut(s) | 1116 |
| Bse8I | GATNNNNATC | 1 cut(s) | 29 |
| BseBI | CCWGG | 3 cut(s) | 312, 373, 1050 |
| BseDI | CCNNGG | 6 cut(s) | 310, 311, 372, 437, 478, 616 |
| BseGI | GGATG | 3 cut(s) | 274, 453, 1224 |
| BseJI | GATNNNNATC | 1 cut(s) | 29 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1148, 1198, 1294 |
| BseMI | GCAATG | 1 cut(s) | 1116 |
| BseMII | CTCAG | 3 cut(s) | 849, 974, 1176 |
| BseRI | GAGGAG | 1 cut(s) | 507 |
| BseSI | GKGCMC | 1 cut(s) | 1228 |
| BseXI | GCAGC | 1 cut(s) | 1070 |
| BsgI | GTGCAG | 1 cut(s) | 1060 |
| BshFI | GGCC | 3 cut(s) | 498, 621, 1141 |
| BshTI | ACCGGT | 1 cut(s) | 670 |
| BsiSI | CCGG | 3 cut(s) | 12, 48, 671 |
| BslFI | GGGAC | 3 cut(s) | 232, 496, 754 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1148, 1198, 1294 |
| BsmFI | GGGAC | 3 cut(s) | 232, 496, 754 |
| BsmI | GAATGC | 1 cut(s) | 525 |
| BsnI | GGCC | 3 cut(s) | 498, 621, 1141 |
| Bsp1286I | GDGCHC | 2 cut(s) | 318, 1228 |
| BspACI | CCGC | 2 cut(s) | 5, 266 |
| BspANI | GGCC | 3 cut(s) | 498, 621, 1141 |
| BspCNI | CTCAG | 3 cut(s) | 850, 973, 1177 |
| BspHI | TCATGA | 1 cut(s) | 529 |
| BspLI | GGNNCC | 1 cut(s) | 586 |
| BspPI | GGATC | 5 cut(s) | 882, 974, 981, 1016, 1258 |
| BspQI | GCTCTTC | 1 cut(s) | 1293 |
| BsrDI | GCAATG | 1 cut(s) | 1116 |
| BsrFI | RCCGGY | 2 cut(s) | 47, 670 |
| BssAI | RCCGGY | 2 cut(s) | 47, 670 |
| BssECI | CCNNGG | 6 cut(s) | 310, 311, 372, 437, 478, 616 |
| BssNI | GRCGYC | 2 cut(s) | 45, 613 |
| BssSI | CACGAG | 1 cut(s) | 489 |
| BssT1I | CCWWGG | 2 cut(s) | 437, 478 |
| Bst2BI | CACGAG | 1 cut(s) | 489 |
| Bst2UI | CCWGG | 3 cut(s) | 312, 373, 1050 |
| Bst4CI | ACNGT | 4 cut(s) | 178, 325, 691, 752 |
| Bst6I | CTCTTC | 1 cut(s) | 1293 |
| BstACI | GRCGYC | 2 cut(s) | 45, 613 |
| BstC8I | GCNNGC | 4 cut(s) | 234, 279, 893, 1086 |
| BstDEI | CTNAG | 4 cut(s) | 680, 858, 960, 1185 |
| BstDSI | CCRYGG | 1 cut(s) | 616 |
| BstF5I | GGATG | 3 cut(s) | 274, 453, 1224 |
| BstH2I | RGCGCY | 1 cut(s) | 1192 |
| BstHHI | GCGC | 1 cut(s) | 1191 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 863, 1094 |
| BstNI | CCWGG | 3 cut(s) | 312, 373, 1050 |
| BstNSI | RCATGY | 2 cut(s) | 281, 361 |
| BstSCI | CCNGG | 3 cut(s) | 310, 371, 1048 |
| BstSFI | CTRYAG | 1 cut(s) | 141 |
| BstSLI | GKGCMC | 1 cut(s) | 1228 |
| BstV1I | GCAGC | 1 cut(s) | 1070 |
| BstV2I | GAAGAC | 1 cut(s) | 1159 |
| BstX2I | RGATCY | 2 cut(s) | 874, 1021 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 464 |
| BstYI | RGATCY | 2 cut(s) | 874, 1021 |
| BsuRI | GGCC | 3 cut(s) | 498, 621, 1141 |
| BtgI | CCRYGG | 1 cut(s) | 616 |
| BtsCI | GGATG | 3 cut(s) | 274, 453, 1224 |
| BtsI | GCAGTG | 2 cut(s) | 116, 1079 |
| BtsIMutI | CAGTG | 3 cut(s) | 116, 174, 1079 |
| Cac8I | GCNNGC | 4 cut(s) | 234, 279, 893, 1086 |
| CciI | TCATGA | 1 cut(s) | 529 |
| CfoI | GCGC | 1 cut(s) | 1191 |
| Cfr10I | RCCGGY | 2 cut(s) | 47, 670 |
| Cfr13I | GGNCC | 2 cut(s) | 239, 1139 |
| CseI | GACGC | 2 cut(s) | 53, 621 |
| Csp6I | GTAC | 4 cut(s) | 471, 674, 808, 1348 |
| CspAI | ACCGGT | 1 cut(s) | 670 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 448, 483 |
| CviAII | CATG | 9 cut(s) | 278, 358, 500, 530, 548, 708, 901, 1033, 1103 |
| CviQI | GTAC | 4 cut(s) | 471, 674, 808, 1348 |
| DdeI | CTNAG | 4 cut(s) | 680, 858, 960, 1185 |
| Eam1104I | CTCTTC | 1 cut(s) | 1293 |
| EarI | CTCTTC | 1 cut(s) | 1293 |
| EciI | GGCGGA | 1 cut(s) | 281 |
| Eco130I | CCWWGG | 2 cut(s) | 437, 478 |
| Eco24I | GRGCYC | 1 cut(s) | 318 |
| Eco32I | GATATC | 1 cut(s) | 957 |
| Eco47I | GGWCC | 1 cut(s) | 239 |
| Eco57I | CTGAAG | 3 cut(s) | 41, 717, 903 |
| EcoRI | GAATTC | 1 cut(s) | 642 |
| EcoRII | CCWGG | 3 cut(s) | 310, 371, 1048 |
| EcoRV | GATATC | 1 cut(s) | 957 |
| EcoT14I | CCWWGG | 2 cut(s) | 437, 478 |
| EcoT38I | GRGCYC | 1 cut(s) | 318 |
| ErhI | CCWWGG | 2 cut(s) | 437, 478 |
| FaeI | CATG | 9 cut(s) | 281, 361, 503, 533, 551, 711, 904, 1036, 1106 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 783, 815, 962, 994, 1144, 1176 |
| FaqI | GGGAC | 3 cut(s) | 232, 496, 754 |
| FatI | CATG | 9 cut(s) | 277, 357, 499, 529, 547, 707, 900, 1032, 1102 |
| FauNDI | CATATG | 1 cut(s) | 840 |
| FbaI | TGATCA | 2 cut(s) | 327, 1035 |
| FblI | GTMKAC | 2 cut(s) | 712, 1071 |
| Fnu4HI | GCNGC | 1 cut(s) | 1059 |
| FokI | GGATG | 3 cut(s) | 281, 440, 1231 |
| FriOI | GRGCYC | 1 cut(s) | 318 |
| Fsp4HI | GCNGC | 1 cut(s) | 1059 |
| FspBI | CTAG | 3 cut(s) | 438, 1319, 1351 |
| GlaI | GCGC | 1 cut(s) | 1190 |
| GluI | GCNGC | 1 cut(s) | 1059 |
| HaeII | RGCGCY | 1 cut(s) | 1192 |
| HaeIII | GGCC | 3 cut(s) | 498, 621, 1141 |
| HapII | CCGG | 3 cut(s) | 12, 48, 671 |
| HgaI | GACGC | 2 cut(s) | 53, 621 |
| HhaI | GCGC | 1 cut(s) | 1191 |
| Hin1I | GRCGYC | 2 cut(s) | 45, 613 |
| Hin1II | CATG | 9 cut(s) | 281, 361, 503, 533, 551, 711, 904, 1036, 1106 |
| Hin6I | GCGC | 1 cut(s) | 1189 |
| HinP1I | GCGC | 1 cut(s) | 1189 |
| HincII | GTYRAC | 3 cut(s) | 163, 432, 520 |
| HindII | GTYRAC | 3 cut(s) | 163, 432, 520 |
| HinfI | GANTC | 4 cut(s) | 760, 767, 822, 1230 |
| HpaI | GTTAAC | 1 cut(s) | 163 |
| HpaII | CCGG | 3 cut(s) | 12, 48, 671 |
| HphI | GGTGA | 4 cut(s) | 26, 337, 361, 578 |
| Hpy188I | TCNGA | 5 cut(s) | 21, 94, 886, 963, 1011 |
| Hpy99I | CGWCG | 2 cut(s) | 47, 83 |
| HpyAV | CCTTC | 4 cut(s) | 236, 779, 784, 1316 |
| HpyCH4III | ACNGT | 4 cut(s) | 178, 325, 691, 752 |
| HpyCH4IV | ACGT | 2 cut(s) | 54, 463 |
| HpyCH4V | TGCA | 6 cut(s) | 121, 277, 895, 983, 1058, 1077 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 863, 1094 |
| HpyF3I | CTNAG | 4 cut(s) | 680, 858, 960, 1185 |
| HpySE526I | ACGT | 2 cut(s) | 54, 463 |
| Hsp92I | GRCGYC | 2 cut(s) | 45, 613 |
| Hsp92II | CATG | 9 cut(s) | 281, 361, 503, 533, 551, 711, 904, 1036, 1106 |
| HspAI | GCGC | 1 cut(s) | 1189 |
| Ksp22I | TGATCA | 2 cut(s) | 327, 1035 |
| KspAI | GTTAAC | 1 cut(s) | 163 |
| LguI | GCTCTTC | 1 cut(s) | 1293 |
| LmnI | GCTCC | 3 cut(s) | 584, 863, 1106 |
| Lsp1109I | GCAGC | 1 cut(s) | 1070 |
| LweI | GCATC | 1 cut(s) | 904 |
| MaeI | CTAG | 3 cut(s) | 438, 1319, 1351 |
| MaeII | ACGT | 2 cut(s) | 54, 463 |
| MaeIII | GTNAC | 3 cut(s) | 50, 289, 367 |
| MflI | RGATCY | 2 cut(s) | 874, 1021 |
| MhlI | GDGCHC | 2 cut(s) | 318, 1228 |
| MluCI | AATT | 5 cut(s) | 540, 558, 642, 693, 1330 |
| MlyI | GAGTC | 3 cut(s) | 776, 816, 1224 |
| MmeI | TCCRAC | 1 cut(s) | 1331 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1156 |
| MseI | TTAA | 4 cut(s) | 162, 543, 1005, 1173 |
| MslI | CAYNNNNRTG | 3 cut(s) | 360, 462, 534 |
| MspA1I | CMGCKG | 1 cut(s) | 1061 |
| MspI | CCGG | 3 cut(s) | 12, 48, 671 |
| MspR9I | CCNGG | 3 cut(s) | 312, 373, 1050 |
| Mva1269I | GAATGC | 1 cut(s) | 525 |
| MvaI | CCWGG | 3 cut(s) | 312, 373, 1050 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 863, 1094 |
| NdeI | CATATG | 1 cut(s) | 840 |
| NlaIII | CATG | 9 cut(s) | 281, 361, 503, 533, 551, 711, 904, 1036, 1106 |
| NlaIV | GGNNCC | 1 cut(s) | 586 |
| NmeAIII | GCCGAG | 1 cut(s) | 1278 |
| NmuCI | GTSAC | 2 cut(s) | 289, 367 |
| NspI | RCATGY | 2 cut(s) | 281, 361 |
| OliI | CACNNNNGTG | 1 cut(s) | 462 |
| PaeI | GCATGC | 1 cut(s) | 281 |
| PagI | TCATGA | 1 cut(s) | 529 |
| PasI | CCCWGGG | 1 cut(s) | 311 |
| PciI | ACATGT | 1 cut(s) | 357 |
| PciSI | GCTCTTC | 1 cut(s) | 1293 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 39, 51 |
| PctI | GAATGC | 1 cut(s) | 525 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1156 |
| PfeI | GAWTC | 1 cut(s) | 760 |
| PflFI | GACNNNGTC | 1 cut(s) | 1067 |
| PinAI | ACCGGT | 1 cut(s) | 670 |
| PkrI | GCNGC | 1 cut(s) | 1060 |
| PleI | GAGTC | 3 cut(s) | 775, 816, 1224 |
| PpsI | GAGTC | 3 cut(s) | 775, 816, 1224 |
| PscI | ACATGT | 1 cut(s) | 357 |
| PshBI | ATTAAT | 1 cut(s) | 1005 |
| Psp6I | CCWGG | 3 cut(s) | 310, 371, 1048 |
| PspGI | CCWGG | 3 cut(s) | 310, 371, 1048 |
| PspN4I | GGNNCC | 1 cut(s) | 586 |
| PspPI | GGNCC | 2 cut(s) | 239, 1139 |
| PsuI | RGATCY | 2 cut(s) | 874, 1021 |
| PsyI | GACNNNGTC | 1 cut(s) | 1067 |
| PvuII | CAGCTG | 1 cut(s) | 1061 |
| RsaI | GTAC | 4 cut(s) | 472, 675, 809, 1349 |
| RsaNI | GTAC | 4 cut(s) | 471, 674, 808, 1348 |
| RseI | CAYNNNNRTG | 3 cut(s) | 360, 462, 534 |
| SapI | GCTCTTC | 1 cut(s) | 1293 |
| SaqAI | TTAA | 4 cut(s) | 162, 543, 1005, 1173 |
| SatI | GCNGC | 1 cut(s) | 1059 |
| Sau96I | GGNCC | 2 cut(s) | 239, 1139 |
| ScaI | AGTACT | 1 cut(s) | 1349 |
| SchI | GAGTC | 3 cut(s) | 776, 816, 1224 |
| ScrFI | CCNGG | 3 cut(s) | 312, 373, 1050 |
| SduI | GDGCHC | 2 cut(s) | 318, 1228 |
| SfaNI | GCATC | 1 cut(s) | 904 |
| SfcI | CTRYAG | 1 cut(s) | 141 |
| SinI | GGWCC | 1 cut(s) | 239 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 360, 462, 534 |
| SphI | GCATGC | 1 cut(s) | 281 |
| Sse9I | AATT | 5 cut(s) | 540, 558, 642, 693, 1330 |
| SsiI | CCGC | 2 cut(s) | 5, 266 |
| SspI | AATATT | 1 cut(s) | 155 |
| SspMI | CTAG | 3 cut(s) | 438, 1319, 1351 |
| StyD4I | CCNGG | 3 cut(s) | 310, 371, 1048 |
| StyI | CCWWGG | 2 cut(s) | 437, 478 |
| TaaI | ACNGT | 4 cut(s) | 178, 325, 691, 752 |
| TaiI | ACGT | 2 cut(s) | 57, 466 |
| TaqI | TCGA | 4 cut(s) | 60, 78, 509, 739 |
| TasI | AATT | 5 cut(s) | 540, 558, 642, 693, 1330 |
| TatI | WGTACW | 2 cut(s) | 807, 1347 |
| TfiI | GAWTC | 1 cut(s) | 760 |
| Tru1I | TTAA | 4 cut(s) | 162, 543, 1005, 1173 |
| Tru9I | TTAA | 4 cut(s) | 162, 543, 1005, 1173 |
| TscAI | CASTG | 3 cut(s) | 123, 181, 1086 |
| TseFI | GTSAC | 2 cut(s) | 289, 367 |
| TseI | GCWGC | 1 cut(s) | 1058 |
| Tsp45I | GTSAC | 2 cut(s) | 289, 367 |
| TspDTI | ATGAA | 6 cut(s) | 41, 285, 518, 696, 917, 1091 |
| TspRI | CASTG | 3 cut(s) | 123, 181, 1086 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1067 |
| VpaK11BI | GGWCC | 1 cut(s) | 239 |
| VspI | ATTAAT | 1 cut(s) | 1005 |
| XapI | RAATTY | 1 cut(s) | 642 |
| XceI | RCATGY | 2 cut(s) | 281, 361 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 464 |
| XmaJI | CCTAGG | 1 cut(s) | 437 |
| XmiI | GTMKAC | 2 cut(s) | 712, 1071 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1156 |
| XspI | CTAG | 3 cut(s) | 438, 1319, 1351 |
| ZrmI | AGTACT | 1 cut(s) | 1349 |