RLG00000019912

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000762
Physical Location & Seq
Forward (+)
58455682 .. 58460800
5119 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019912

Sequence Viewer

Length: 1614 bp
ATGGCCAACTCTGCTTCTTCCATTTTCACCACCACTCCACGGTTCCGCTGCTTCGACCCCACCAACTCTTACGCTGCGACGCCCGATCACCCCCGCCGCTTCAGCACCACCTCCAATTCAACTCCCAGGTACTACAACAACAACAACAACAACAACTTCAAGGGCACTATGGGATTTTCCGCCAACTCCGAGCCCAAGATCATCACCGGCGACGCTGGCTACGTTCTCGAAGACGTCCCTCACTTGTCCGATTACATCCCCGATCTCCCTACATATCCCAATCCATTACAGGACAATCCTTCCTATTCAGTTGTTAAGCAGTACTTCGTTAATGTCGATGATAGTGTTGCCCAGACGATTGTAGTCCAGAAAGATAGTCCAAGAGGCACACATTTTCGACGTGCTGGACCGCGTCAGAAGGTCTATTTTGAACCAGATGATGTTCGTGCGTGTATTGTGACATGTGGTGGTTTATGTCCAGGCTTAAATACAGTAATTAGAGAAATAGTATGTGGGTTGTACCACATGTATGGTGTCCACCGAATTCTTGGAATAGAGGGAGGATACAGAGGGTTTTATGCCAGAAATACAATTCCCTTAACACCGAAGGTTGTGAATGACATCCATAAACGTGGTGGCACCATCCTTGGGACATCACGAGGTGGCCATGATACCTCAAAGATTGTGGACAGCATTCAGGACAGAGGAATTAATCAGGTATACATACTTGGGGGAGATGGTACACAGAAAGGAGCTTCCGTAATATTTGAGGAATGCAGGAGGCGTGGCCTCAATGTTGTAGTGGCAGGAATTCCAAAAACCATTGATAATGACATTCCGTTATCCAATTTAAGAGTATTTTACAGAACATCAACTGAAAAGGGGATCACAAGGTTATGTTTAGGGGTATGCCTCCTGTTTCCTTATTATCTGATATTATCTTGTCATACAGGATTTATAGCTATGTATGCTACTCTTGCCAGCCGAGATGTGGATTGTTGCCTGATTCCAGAGTCGCCTTTCTATCTGGAAGGAGCAGGTGGATTGTTTGAGTACATTGAAAAAAGACTTAAAGAGAACGGTCACATGGTTATAGTAATAGCTGAAGGCGCAGGACAAGAGCTTCTAGCTGAAAGCAGGCTTGCTATGAATCATCAGGACGCTTCTGGAAATAAGCTTCTTCAAGATTCTGGTCTGTGGTTATCGCAGAAGATTAAGGACCACTTCTCAAAACTGAACAAGATGTCTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCTATTCCAAGCAATGCATCTGATAATGTATACTGCACCCTCCTTGCTCATAGTGTGGTTCACGGTGCAATGGCAGGATACACAGGCTTCACTGTTGGCCCTGTTAATGGAAGGCATGCTTATATTCCTTTCCATCGGGTGACAGAGAGACAGAACAAGGTTGTGATTACTGACAGAATGTGGGCACGGCTATTATCATCAACTAATCAGCCAAGTTTCTTGGATTTGAAGGAAGTAGCTAAAAAGAAAGGGGAAGAAACAGAAACCCACCTGGTCAATGGGGAAAACCATTTGGAGGAAACTGCTTCGGCTACGCCAAAGGAGTAG

Protein Analysis

538

Amino Acids

59.55

Weight (kDa)

8.32

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 150 - 286 8.9e-31 Phosphofructokinase
PFK PF00365 316 - 450 3.4e-09 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1028
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 1028
AccB1I GGYRCC 1 cut(s) 638
AccI GTMKAC 2 cut(s) 720, 1317
AccII CGCG 1 cut(s) 412
AciI CCGC 5 cut(s) 46, 94, 97, 180, 410
AclWI GGATC 3 cut(s) 893, 1262, 1276
AcoI YGGCCR 2 cut(s) 3, 664
AcsI RAATTY 2 cut(s) 543, 810
AcuI CTGAAG 2 cut(s) 85, 1125
AcyI GRCGYC 2 cut(s) 80, 234
AdeI CACNNNGTG 1 cut(s) 662
AfaI GTAC 5 cut(s) 131, 323, 521, 742, 1055
AfiI CCNNNNNNNGG 5 cut(s) 39, 648, 991, 1394, 1582
AflIII ACRYGT 2 cut(s) 461, 525
AgsI TTSAA 6 cut(s) 120, 160, 431, 1061, 1184, 1516
AjiI CACGTC 1 cut(s) 401
AjnI CCWGG 3 cut(s) 125, 478, 1557
AluBI AGCT 7 cut(s) 755, 962, 1103, 1123, 1130, 1177, 1526
AluI AGCT 7 cut(s) 755, 962, 1103, 1123, 1130, 1177, 1526
Alw26I GTCTC 1 cut(s) 1429
AlwI GGATC 3 cut(s) 893, 1262, 1276
AoxI GGCC 4 cut(s) 3, 664, 787, 1384
ApeKI GCWGC 2 cut(s) 48, 74
ApoI RAATTY 2 cut(s) 543, 810
AseI ATTAAT 1 cut(s) 711
AspLEI GCGC 1 cut(s) 1112
AspS9I GGNCC 3 cut(s) 407, 1219, 1385
AsuHPI GGTGA 4 cut(s) 19, 80, 196, 1438
AvaII GGWCC 2 cut(s) 407, 1219
BaeGI GKGCMC 2 cut(s) 167, 1474
BalI TGGCCA 2 cut(s) 5, 666
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 1 cut(s) 195
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 35, 61
BccI CCATC 3 cut(s) 650, 731, 1428
BceAI ACGGC 1 cut(s) 1490
BciT130I CCWGG 3 cut(s) 127, 480, 1559
BciVI GTATCC 2 cut(s) 557, 1358
BcoDI GTCTC 1 cut(s) 1429
BfaI CTAG 1 cut(s) 1127
BfuAI ACCTGC 1 cut(s) 1028
BfuI GTATCC 2 cut(s) 557, 1358
BisI GCNGC 3 cut(s) 49, 75, 97
BlsI GCNGC 3 cut(s) 50, 76, 98
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 3 cut(s) 127, 480, 1559
Bme18I GGWCC 2 cut(s) 407, 1219
BmgBI CACGTC 1 cut(s) 401
BmgT120I GGNCC 3 cut(s) 407, 1219, 1385
BmiI GGNNCC 2 cut(s) 44, 640
BmrFI CCNGG 3 cut(s) 127, 480, 1559
BmsI GCATC 1 cut(s) 1313
BpiI GAAGAC 1 cut(s) 237
BsaHI GRCGYC 2 cut(s) 80, 234
BsaJI CCNNGG 3 cut(s) 38, 125, 646
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 744, 774
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 648, 991, 1394, 1582
Bse118I RCCGGY 1 cut(s) 206
Bse3DI GCAATG 2 cut(s) 1306, 1362
BseBI CCWGG 3 cut(s) 127, 480, 1559
BseDI CCNNGG 3 cut(s) 38, 125, 646
BseGI GGATG 3 cut(s) 255, 621, 642
BseLI CCNNNNNNNGG 5 cut(s) 39, 648, 991, 1394, 1582
BseMI GCAATG 2 cut(s) 1306, 1362
BseSI GKGCMC 2 cut(s) 167, 1474
BseXI GCAGC 2 cut(s) 35, 61
BsgI GTGCAG 1 cut(s) 1306
Bsh1236I CGCG 1 cut(s) 412
BshFI GGCC 4 cut(s) 5, 666, 789, 1386
BshNI GGYRCC 1 cut(s) 638
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 221, 664
BslI CCNNNNNNNGG 5 cut(s) 39, 648, 991, 1394, 1582
BsmAI GTCTC 1 cut(s) 1429
BsmFI GGGAC 2 cut(s) 221, 664
BsmI GAATGC 2 cut(s) 693, 779
BsnI GGCC 4 cut(s) 5, 666, 789, 1386
Bsp1286I GDGCHC 3 cut(s) 167, 195, 1474
Bsp143I GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
BspACI CCGC 5 cut(s) 46, 94, 97, 180, 410
BspANI GGCC 4 cut(s) 5, 666, 789, 1386
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 44, 640
BspMI ACCTGC 1 cut(s) 1028
BspPI GGATC 3 cut(s) 893, 1262, 1276
BspT107I GGYRCC 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 1306, 1362
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 38, 125, 646
BssMI GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
BssNAI GTATAC 2 cut(s) 721, 1318
BssNI GRCGYC 2 cut(s) 80, 234
BssSI CACGAG 1 cut(s) 657
BssT1I CCWWGG 1 cut(s) 646
Bst1107I GTATAC 2 cut(s) 721, 1318
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 3 cut(s) 127, 480, 1559
Bst4CI ACNGT 5 cut(s) 42, 493, 1082, 1352, 1381
BstACI GRCGYC 2 cut(s) 80, 234
BstC8I GCNNGC 5 cut(s) 217, 982, 1139, 1143, 1404
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 255, 621, 642
BstFNI CGCG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 1112
BstKTI GATC 6 cut(s) 88, 201, 265, 888, 1270, 1284
BstMAI GTCTC 1 cut(s) 1429
BstMBI GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
BstMWI GCNNNNNNNGC 6 cut(s) 11, 102, 216, 968, 977, 1109
BstNI CCWGG 3 cut(s) 127, 480, 1559
BstNSI RCATGY 3 cut(s) 465, 529, 1406
BstSCI CCNGG 3 cut(s) 125, 478, 1557
BstSLI GKGCMC 2 cut(s) 167, 1474
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 35, 61
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 1267
BstXI CCANNNNNNTGG 2 cut(s) 530, 632
BstYI RGATCY 1 cut(s) 1267
BstZ17I GTATAC 2 cut(s) 721, 1318
BsuI GTATCC 2 cut(s) 557, 1358
BsuRI GGCC 4 cut(s) 5, 666, 789, 1386
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 401
BtsCI GGATG 3 cut(s) 255, 621, 642
BtsIMutI CAGTG 1 cut(s) 1377
BveI ACCTGC 1 cut(s) 1028
Cac8I GCNNGC 5 cut(s) 217, 982, 1139, 1143, 1404
CfoI GCGC 1 cut(s) 1112
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 3 cut(s) 407, 1219, 1385
CseI GACGC 4 cut(s) 88, 221, 401, 1169
CsiI ACCWGGT 1 cut(s) 1557
Csp6I GTAC 5 cut(s) 130, 322, 520, 741, 1054
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviAII CATG 6 cut(s) 462, 526, 668, 1087, 1279, 1403
CviQI GTAC 5 cut(s) 130, 322, 520, 741, 1054
DpnI GATC 6 cut(s) 87, 200, 264, 887, 1269, 1283
DpnII GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
DraIII CACNNNGTG 1 cut(s) 662
EaeI YGGCCR 2 cut(s) 3, 664
EciI GGCGGA 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 646
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 407, 1219
Eco57I CTGAAG 2 cut(s) 85, 1125
EcoRI GAATTC 2 cut(s) 543, 810
EcoRII CCWGG 3 cut(s) 125, 478, 1557
EcoT14I CCWWGG 1 cut(s) 646
EcoT22I ATGCAT 1 cut(s) 1306
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 646
FaeI CATG 6 cut(s) 465, 529, 671, 1090, 1282, 1406
FalI AAGNNNNNCTT 6 cut(s) 308, 340, 1208, 1240, 1390, 1422
FaqI GGGAC 2 cut(s) 221, 664
FatI CATG 6 cut(s) 461, 525, 667, 1086, 1278, 1402
FauI CCCGC 1 cut(s) 101
FblI GTMKAC 2 cut(s) 720, 1317
Fnu4HI GCNGC 3 cut(s) 49, 75, 97
FokI GGATG 3 cut(s) 242, 608, 629
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 49, 75, 97
FspBI CTAG 1 cut(s) 1127
GlaI GCGC 1 cut(s) 1111
GluI GCNGC 3 cut(s) 49, 75, 97
HaeIII GGCC 4 cut(s) 5, 666, 789, 1386
HapII CCGG 1 cut(s) 207
HgaI GACGC 4 cut(s) 88, 221, 401, 1169
HhaI GCGC 1 cut(s) 1112
Hin1I GRCGYC 2 cut(s) 80, 234
Hin1II CATG 6 cut(s) 465, 529, 671, 1090, 1282, 1406
Hin6I GCGC 1 cut(s) 1110
HinP1I GCGC 1 cut(s) 1110
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 4 cut(s) 1006, 1013, 1150, 1187
HpaII CCGG 1 cut(s) 207
HphI GGTGA 4 cut(s) 19, 80, 196, 1438
Hpy166II GTNNAC 6 cut(s) 538, 688, 721, 743, 1318, 1348
Hpy188I TCNGA 5 cut(s) 190, 250, 417, 933, 1309
Hpy188III TCNNGA 9 cut(s) 227, 367, 657, 698, 1010, 1028, 1157, 1167, 1184
Hpy8I GTNNAC 6 cut(s) 538, 688, 721, 743, 1318, 1348
Hpy99I CGWCG 3 cut(s) 82, 215, 402
HpyAV CCTTC 7 cut(s) 309, 412, 601, 1025, 1100, 1392, 1510
HpyCH4III ACNGT 5 cut(s) 42, 493, 1082, 1352, 1381
HpyCH4IV ACGT 4 cut(s) 222, 234, 400, 631
HpyCH4V TGCA 4 cut(s) 777, 1304, 1323, 1355
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 102, 216, 968, 977, 1109
HpySE526I ACGT 4 cut(s) 222, 234, 400, 631
Hsp92I GRCGYC 2 cut(s) 80, 234
Hsp92II CATG 6 cut(s) 465, 529, 671, 1090, 1282, 1406
HspAI GCGC 1 cut(s) 1110
Kzo9I GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
LmnI GCTCC 2 cut(s) 752, 1034
Lsp1109I GCAGC 2 cut(s) 35, 61
LweI GCATC 1 cut(s) 1313
MabI ACCWGGT 1 cut(s) 1557
MaeI CTAG 1 cut(s) 1127
MaeII ACGT 4 cut(s) 222, 234, 400, 631
MaeIII GTNAC 3 cut(s) 457, 1082, 1426
MalI GATC 6 cut(s) 87, 200, 264, 887, 1269, 1283
MboI GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
MboII GAAGA 5 cut(s) 9, 242, 1172, 1222, 1553
MflI RGATCY 1 cut(s) 1267
MhlI GDGCHC 3 cut(s) 167, 195, 1474
MlsI TGGCCA 2 cut(s) 5, 666
MluCI AATT 7 cut(s) 115, 495, 543, 591, 708, 810, 847
MluNI TGGCCA 2 cut(s) 5, 666
MlyI GAGTC 1 cut(s) 1022
Mox20I TGGCCA 2 cut(s) 5, 666
Mph1103I ATGCAT 1 cut(s) 1306
MscI TGGCCA 2 cut(s) 5, 666
MseI TTAA 9 cut(s) 315, 330, 485, 599, 711, 851, 1071, 1215, 1392
MslI CAYNNNNRTG 2 cut(s) 528, 630
Msp20I TGGCCA 2 cut(s) 5, 666
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 3 cut(s) 127, 480, 1559
Mva1269I GAATGC 2 cut(s) 693, 779
MvaI CCWGG 3 cut(s) 127, 480, 1559
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 6 cut(s) 11, 102, 216, 968, 977, 1109
NdeII GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
NlaIII CATG 6 cut(s) 465, 529, 671, 1090, 1282, 1406
NlaIV GGNNCC 2 cut(s) 44, 640
NmeAIII GCCGAG 1 cut(s) 1010
NmuCI GTSAC 3 cut(s) 457, 1082, 1426
NsiI ATGCAT 1 cut(s) 1306
NspI RCATGY 3 cut(s) 465, 529, 1406
PaeI GCATGC 1 cut(s) 1406
PaqCI CACCTGC 1 cut(s) 1028
PciI ACATGT 2 cut(s) 461, 525
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 333
PctI GAATGC 2 cut(s) 693, 779
PfeI GAWTC 3 cut(s) 1006, 1150, 1187
PflFI GACNNNGTC 1 cut(s) 411
PkrI GCNGC 3 cut(s) 50, 76, 98
PleI GAGTC 1 cut(s) 1021
PpsI GAGTC 1 cut(s) 1021
PscI ACATGT 2 cut(s) 461, 525
PshBI ATTAAT 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 125, 478, 1557
PspGI CCWGG 3 cut(s) 125, 478, 1557
PspN4I GGNNCC 2 cut(s) 44, 640
PspPI GGNCC 3 cut(s) 407, 1219, 1385
PsuI RGATCY 1 cut(s) 1267
PsyI GACNNNGTC 1 cut(s) 411
RsaI GTAC 5 cut(s) 131, 323, 521, 742, 1055
RsaNI GTAC 5 cut(s) 130, 322, 520, 741, 1054
RseI CAYNNNNRTG 2 cut(s) 528, 630
SaqAI TTAA 9 cut(s) 315, 330, 485, 599, 711, 851, 1071, 1215, 1392
SatI GCNGC 3 cut(s) 49, 75, 97
Sau3AI GATC 6 cut(s) 85, 198, 262, 885, 1267, 1281
Sau96I GGNCC 3 cut(s) 407, 1219, 1385
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 3 cut(s) 127, 480, 1559
SduI GDGCHC 3 cut(s) 167, 195, 1474
SexAI ACCWGGT 1 cut(s) 1557
SfaNI GCATC 1 cut(s) 1313
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 2 cut(s) 407, 1219
SmiMI CAYNNNNRTG 2 cut(s) 528, 630
SphI GCATGC 1 cut(s) 1406
Sse9I AATT 7 cut(s) 115, 495, 543, 591, 708, 810, 847
SsiI CCGC 5 cut(s) 46, 94, 97, 180, 410
SspI AATATT 1 cut(s) 765
SspMI CTAG 1 cut(s) 1127
StyD4I CCNGG 3 cut(s) 125, 478, 1557
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 5 cut(s) 42, 493, 1082, 1352, 1381
TaiI ACGT 4 cut(s) 225, 237, 403, 634
TaqI TCGA 4 cut(s) 54, 228, 336, 397
TasI AATT 7 cut(s) 115, 495, 543, 591, 708, 810, 847
TatI WGTACW 2 cut(s) 321, 1053
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 3 cut(s) 1006, 1150, 1187
Tru1I TTAA 9 cut(s) 315, 330, 485, 599, 711, 851, 1071, 1215, 1392
Tru9I TTAA 9 cut(s) 315, 330, 485, 599, 711, 851, 1071, 1215, 1392
TscAI CASTG 1 cut(s) 1384
TseFI GTSAC 3 cut(s) 457, 1082, 1426
TseI GCWGC 2 cut(s) 48, 74
Tsp45I GTSAC 3 cut(s) 457, 1082, 1426
TspDTI ATGAA 1 cut(s) 1163
TspGWI ACGGA 3 cut(s) 748, 828, 1274
TspRI CASTG 1 cut(s) 1384
Tth111I GACNNNGTC 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 407, 1219
VspI ATTAAT 1 cut(s) 711
XapI RAATTY 2 cut(s) 543, 810
XceI RCATGY 3 cut(s) 465, 529, 1406
XcmI CCANNNNNNNNNTGG 4 cut(s) 545, 632, 988, 1562
XmiI GTMKAC 2 cut(s) 720, 1317
XspI CTAG 1 cut(s) 1127
ZraI GACGTC 1 cut(s) 235
ZrmI AGTACT 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.