pycom09g11720

6-phosphofructokinase 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000762
Physical Location & Seq
Reverse (-)
9917423 .. 9922479
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11720.10

Sequence Viewer

Length: 1560 bp
ATGGCCAGCACCGCTTCCATTTCTAGGCCCAGGTTCCGCTGCTTCGACCCTACTAACTCTTACACTGCGACTCCCGATCACCCTCGCTACTTCTCGGCTTCCCCTTCCACCTCCAGCCCCTCCACTCCCCGAGTGAACGGCCGCTCCAGTGGCAGCATGGCAATTTCCGACAACTCCGAGCCCAAAATCGCCACGGGCCCCGGTGGCTACGTCCTCGAAGATGTTCCTCACTTAGTTGATTACATCCCCGATCTCCCCACATATCCCAATCCATTACAAGACAATCCGTCCTATTCGATTGTTGTTCATATGGATAGTCCAAGGGGGACACACTTTCGACGCTCTGGACCACGTCAGAAGGTGTATTTCGAGTCAAATGATGTGCATGCGTGCATTGTGACATGTGGTGGTCTATGTCCAGGACTGAATACAGTTATTAGGGAAATAGTATGCGGTCTGTACCACATGTATGGTGTCAAAAGAATTCTTGGAATAGAGGGTGGATATAGAGGGTTTTATGCCAGAAATACAATCCCCTTAACACCGAAGGTTGTGAATGACATCCATAAACGAGGTGGCACCATCCTTGGAACCTCGCGAGGTGGGCATGTTACCTCAAAGATTGTGGACAGCATCCAGGACAGAGGAATTAATCAGGTTTATGTAATTGGAGGAGATGGAACTCAGAAAGGAGCTTCGGTGATTTTTGAGGAAATCAGGAGGCGTGGCCTCAAAGTAGTGGTGGCAGGAATTCCCAAAACCATCGATAATGACATTCCGGTTATAGACAGGTCTTTTGGTTTCGACACTGCTGTTGAGGAGGCTCAACGAGCCATTAATGCAGCCCATGTTGAAGCAGAAAGCGTTGAGAATGGTATTGGCTTCGTCAAGTTAATGGGCCGCTACAGTGGATTCATAGCAATGTATGCTACTCTTGCTAGCCGAGACGTCGACTGTTGTCTGATTCCAGAATCGCCTTTTTATCTGGAAGGACCAGGTGGACTTTATGAATACATTGAAAGAAGACTGAAGGAGGATGGTCACCTGGTTATAGTAATGGCTGAAGGTGCAGGACAAGAACTTCTTGCTGAAAGCATGAACCAGCAGGATGCTTCCGGAAATAAGCTTCTCCAAGATGTTGGTCTGTGGTTATCGCAAAATATTAGGGACCACTTCTCGAAACAGAGAAATATCACTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCAATTCCAAGCAATGCATCTGATAATGTATATTGCACCCTCCTTGCTCACAGTGCCATTCATGGTGCAATGGCTGGATACACGGGCTTCACTGTCGGCCCTGTTAACGGGAGGCATGCTTACATTCCGTTCCGTTGGGTGACCGAGAAGCAGAACAAGGTTGTGATCACTGACAGAATGTGGGCACGGTTACTGTCGTCAACCAATCAGCCAAGCTTCTTGGACCTCAAGGAGGTTGAAGCAATTAAGAAAGAGGAAAAGGAAGAGGAAACAAAAGCCCAACTGATAGTTGGGGAAAACCATACTGAAGCAACTTCAGCGTAG

Protein Analysis

520

Amino Acids

57.05

Weight (kDa)

6.58

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 951
AccB1I GGYRCC 1 cut(s) 578
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 951
AccII CGCG 1 cut(s) 598
AccIII TCCGGA 1 cut(s) 1115
AciI CCGC 5 cut(s) 12, 37, 142, 453, 901
AclWI GGATC 2 cut(s) 1211, 1225
AcoI YGGCCR 2 cut(s) 3, 139
AcsI RAATTY 2 cut(s) 483, 750
AcuI CTGAAG 3 cut(s) 1049, 1083, 1536
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 24, 1343, 1468
AflIII ACRYGT 2 cut(s) 401, 465
AgsI TTSAA 3 cut(s) 854, 1019, 1475
AhdI GACNNNNNGTC 1 cut(s) 286
AjiI CACGTC 1 cut(s) 353
AjnI CCWGG 5 cut(s) 29, 418, 636, 994, 1044
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 695, 1126, 1452
AluI AGCT 3 cut(s) 695, 1126, 1452
Alw26I GTCTC 1 cut(s) 939
AlwI GGATC 2 cut(s) 1211, 1225
Ama87I CYCGRG 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 1115
AoxI GGCC 7 cut(s) 3, 26, 139, 196, 727, 898, 1333
ApaI GGGCCC 1 cut(s) 200
ApeKI GCWGC 3 cut(s) 39, 153, 842
ApoI RAATTY 2 cut(s) 483, 750
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AseI ATTAAT 2 cut(s) 651, 837
Asp700I GAANNNNTTC 1 cut(s) 222
AspS9I GGNCC 9 cut(s) 27, 196, 197, 347, 898, 992, 1168, 1334, 1459
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 4 cut(s) 71, 712, 1034, 1387
AsuNHI GCTAGC 1 cut(s) 938
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 4 cut(s) 347, 992, 1168, 1459
BaeGI GKGCMC 2 cut(s) 200, 1423
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 2 cut(s) 183, 200
BbsI GAAGAC 1 cut(s) 1030
BbvI GCAGC 3 cut(s) 26, 165, 854
BccI CCATC 4 cut(s) 590, 671, 770, 1031
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 5 cut(s) 31, 420, 638, 996, 1046
BciVI GTATCC 1 cut(s) 1307
BclI TGATCA 1 cut(s) 1401
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 939
BfaI CTAG 2 cut(s) 24, 939
BfmI CTRYAG 1 cut(s) 904
BfuI GTATCC 1 cut(s) 1307
BglI GCCNNNNNGGC 1 cut(s) 204
BisI GCNGC 5 cut(s) 40, 142, 154, 843, 901
BlsI GCNGC 5 cut(s) 41, 143, 155, 844, 902
Bme1390I CCNGG 6 cut(s) 31, 201, 420, 638, 996, 1046
Bme18I GGWCC 4 cut(s) 347, 992, 1168, 1459
BmeRI GACNNNNNGTC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 129
BmgBI CACGTC 1 cut(s) 353
BmgT120I GGNCC 9 cut(s) 27, 196, 197, 347, 898, 992, 1168, 1334, 1459
BmiI GGNNCC 6 cut(s) 35, 198, 199, 580, 592, 1169
BmrFI CCNGG 6 cut(s) 31, 201, 420, 638, 996, 1046
BmsI GCATC 3 cut(s) 642, 1099, 1262
BmtI GCTAGC 1 cut(s) 942
BoxI GACNNNNGTC 1 cut(s) 957
BpiI GAAGAC 1 cut(s) 1030
BpmI CTGGAG 2 cut(s) 97, 130
BpuEI CTTGAG 1 cut(s) 1448
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 948
BsaJI CCNNGG 5 cut(s) 29, 192, 199, 320, 586
BsaWI WCCGGW 2 cut(s) 778, 1115
BsaXI ACNNNNNCTCC 6 cut(s) 55, 85, 128, 158, 684, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 1343, 1468
Bse1I ACTGG 1 cut(s) 147
Bse3DI GCAATG 3 cut(s) 927, 1255, 1311
BseAI TCCGGA 1 cut(s) 1115
BseBI CCWGG 5 cut(s) 31, 420, 638, 996, 1046
BseCI ATCGAT 1 cut(s) 765
BseDI CCNNGG 5 cut(s) 29, 192, 199, 320, 586
BseGI GGATG 6 cut(s) 243, 561, 582, 633, 1042, 1114
BseLI CCNNNNNNNGG 3 cut(s) 24, 1343, 1468
BseMI GCAATG 3 cut(s) 927, 1255, 1311
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 147
BseRI GAGGAG 2 cut(s) 687, 833
BseSI GKGCMC 2 cut(s) 200, 1423
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 26, 165, 854
BsgI GTGCAG 1 cut(s) 1089
Bsh1236I CGCG 1 cut(s) 598
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 7 cut(s) 5, 28, 141, 198, 729, 900, 1335
BshNI GGYRCC 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 765
BsiEI CGRYCG 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 3 cut(s) 201, 779, 1116
BslFI GGGAC 2 cut(s) 340, 1181
BslI CCNNNNNNNGG 3 cut(s) 24, 1343, 1468
BsmAI GTCTC 1 cut(s) 939
BsmBI CGTCTC 1 cut(s) 939
BsmFI GGGAC 2 cut(s) 340, 1181
BsnI GGCC 7 cut(s) 5, 28, 141, 198, 729, 900, 1335
BsoBI CYCGRG 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 3 cut(s) 183, 200, 1423
Bsp13I TCCGGA 1 cut(s) 1115
Bsp143I GATC 5 cut(s) 76, 250, 1216, 1230, 1401
Bsp68I TCGCGA 1 cut(s) 598
BspACI CCGC 5 cut(s) 12, 37, 142, 453, 901
BspANI GGCC 7 cut(s) 5, 28, 141, 198, 729, 900, 1335
BspCNI CTCAG 1 cut(s) 697
BspDI ATCGAT 1 cut(s) 765
BspEI TCCGGA 1 cut(s) 1115
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 6 cut(s) 35, 198, 199, 580, 592, 1169
BspOI GCTAGC 1 cut(s) 942
BspPI GGATC 2 cut(s) 1211, 1225
BspT107I GGYRCC 1 cut(s) 578
BsrBI CCGCTC 1 cut(s) 144
BsrDI GCAATG 3 cut(s) 927, 1255, 1311
BsrI ACTGG 1 cut(s) 147
BssECI CCNNGG 5 cut(s) 29, 192, 199, 320, 586
BssMI GATC 5 cut(s) 76, 250, 1216, 1230, 1401
BssNI GRCGYC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 320, 586
Bst2UI CCWGG 5 cut(s) 31, 420, 638, 996, 1046
Bst4CI ACNGT 7 cut(s) 433, 908, 956, 1289, 1330, 1425, 1431
Bst6I CTCTTC 1 cut(s) 1494
BstACI GRCGYC 1 cut(s) 948
BstAPI GCANNNNNTGC 1 cut(s) 926
BstC8I GCNNGC 5 cut(s) 7, 387, 391, 940, 1353
BstDEI CTNAG 2 cut(s) 232, 684
BstDSI CCRYGG 1 cut(s) 192
BstEII GGTNACC 2 cut(s) 1040, 1375
BstENI CCTNNNNNAGG 1 cut(s) 1466
BstF5I GGATG 6 cut(s) 243, 561, 582, 633, 1042, 1114
BstFNI CGCG 1 cut(s) 598
BstKTI GATC 5 cut(s) 79, 253, 1219, 1233, 1404
BstMAI GTCTC 1 cut(s) 939
BstMBI GATC 5 cut(s) 76, 250, 1216, 1230, 1401
BstMCI CGRYCG 1 cut(s) 142
BstNI CCWGG 5 cut(s) 31, 420, 638, 996, 1046
BstNSI RCATGY 5 cut(s) 389, 405, 469, 611, 1355
BstPAI GACNNNNGTC 1 cut(s) 957
BstPI GGTNACC 2 cut(s) 1040, 1375
BstSCI CCNGG 6 cut(s) 29, 199, 418, 636, 994, 1044
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 2 cut(s) 200, 1423
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 3 cut(s) 26, 165, 854
BstV2I GAAGAC 1 cut(s) 1030
BstX2I RGATCY 1 cut(s) 1216
BstXI CCANNNNNNTGG 2 cut(s) 470, 1139
BstYI RGATCY 1 cut(s) 1216
BstZI CGGCCG 1 cut(s) 139
Bsu15I ATCGAT 1 cut(s) 765
BsuI GTATCC 1 cut(s) 1307
BsuRI GGCC 7 cut(s) 5, 28, 141, 198, 729, 900, 1335
BsuTUI ATCGAT 1 cut(s) 765
BtgI CCRYGG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 353
BtsCI GGATG 6 cut(s) 243, 561, 582, 633, 1042, 1114
BtsI GCAGTG 2 cut(s) 63, 807
BtsIMutI CAGTG 7 cut(s) 63, 154, 807, 913, 1294, 1326, 1404
BtuMI TCGCGA 1 cut(s) 598
Cac8I GCNNGC 5 cut(s) 7, 387, 391, 940, 1353
Cfr13I GGNCC 9 cut(s) 27, 196, 197, 347, 898, 992, 1168, 1334, 1459
ClaI ATCGAT 1 cut(s) 765
CseI GACGC 1 cut(s) 348
CsiI ACCWGGT 2 cut(s) 994, 1044
Csp6I GTAC 1 cut(s) 460
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 2 cut(s) 232, 684
DpnI GATC 5 cut(s) 78, 252, 1218, 1232, 1403
DpnII GATC 5 cut(s) 76, 250, 1216, 1230, 1401
DriI GACNNNNNGTC 1 cut(s) 286
EaeI YGGCCR 2 cut(s) 3, 139
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 1494
Eam1105I GACNNNNNGTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 1494
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 320, 586
Eco24I GRGCYC 2 cut(s) 183, 200
Eco47I GGWCC 4 cut(s) 347, 992, 1168, 1459
Eco52I CGGCCG 1 cut(s) 139
Eco57I CTGAAG 3 cut(s) 1049, 1083, 1536
Eco88I CYCGRG 1 cut(s) 129
Eco91I GGTNACC 2 cut(s) 1040, 1375
EcoNI CCTNNNNNAGG 1 cut(s) 1466
EcoO109I RGGNCCY 1 cut(s) 197
EcoO65I GGTNACC 2 cut(s) 1040, 1375
EcoRI GAATTC 2 cut(s) 483, 750
EcoRII CCWGG 5 cut(s) 29, 418, 636, 994, 1044
EcoT14I CCWWGG 2 cut(s) 320, 586
EcoT22I ATGCAT 1 cut(s) 1255
EcoT38I GRGCYC 2 cut(s) 183, 200
ErhI CCWWGG 2 cut(s) 320, 586
Esp3I CGTCTC 1 cut(s) 939
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 2 cut(s) 340, 1181
FauNDI CATATG 1 cut(s) 309
FbaI TGATCA 1 cut(s) 1401
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 5 cut(s) 40, 142, 154, 843, 901
FokI GGATG 6 cut(s) 230, 548, 569, 620, 1049, 1121
FriOI GRGCYC 2 cut(s) 183, 200
Fsp4HI GCNGC 5 cut(s) 40, 142, 154, 843, 901
FspBI CTAG 2 cut(s) 24, 939
GluI GCNGC 5 cut(s) 40, 142, 154, 843, 901
GsuI CTGGAG 2 cut(s) 97, 130
HaeIII GGCC 7 cut(s) 5, 28, 141, 198, 729, 900, 1335
HapII CCGG 3 cut(s) 201, 779, 1116
HgaI GACGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 948
HincII GTYRAC 3 cut(s) 952, 1342, 1437
HindII GTYRAC 3 cut(s) 952, 1342, 1437
HindIII AAGCTT 2 cut(s) 1124, 1450
HinfI GANTC 5 cut(s) 70, 371, 912, 964, 971
HpaI GTTAAC 1 cut(s) 1342
HpaII CCGG 3 cut(s) 201, 779, 1116
HphI GGTGA 4 cut(s) 71, 712, 1034, 1387
Hpy166II GTNNAC 6 cut(s) 136, 628, 952, 1001, 1342, 1437
Hpy188I TCNGA 6 cut(s) 169, 178, 357, 687, 963, 1258
Hpy188III TCNNGA 8 cut(s) 74, 345, 597, 718, 968, 986, 1116, 1177
Hpy8I GTNNAC 6 cut(s) 136, 628, 952, 1001, 1342, 1437
Hpy99I CGWCG 2 cut(s) 342, 953
HpyAV CCTTC 6 cut(s) 114, 352, 541, 983, 1024, 1058
HpyCH4III ACNGT 7 cut(s) 433, 908, 956, 1289, 1330, 1425, 1431
HpyCH4IV ACGT 3 cut(s) 210, 352, 948
HpyCH4V TGCA 8 cut(s) 385, 393, 842, 1070, 1238, 1253, 1272, 1304
HpyF3I CTNAG 2 cut(s) 232, 684
HpySE526I ACGT 3 cut(s) 210, 352, 948
Hsp92I GRCGYC 1 cut(s) 948
Kpn2I TCCGGA 1 cut(s) 1115
Ksp22I TGATCA 1 cut(s) 1401
KspAI GTTAAC 1 cut(s) 1342
Kzo9I GATC 5 cut(s) 76, 250, 1216, 1230, 1401
LmnI GCTCC 2 cut(s) 149, 692
Lsp1109I GCAGC 3 cut(s) 26, 165, 854
LweI GCATC 3 cut(s) 642, 1099, 1262
MabI ACCWGGT 2 cut(s) 994, 1044
MaeI CTAG 2 cut(s) 24, 939
MaeII ACGT 3 cut(s) 210, 352, 948
MaeIII GTNAC 5 cut(s) 397, 610, 1040, 1375, 1425
MalI GATC 5 cut(s) 78, 252, 1218, 1232, 1403
MbiI CCGCTC 1 cut(s) 144
MboI GATC 5 cut(s) 76, 250, 1216, 1230, 1401
MboII GAAGA 3 cut(s) 230, 1035, 1511
MflI RGATCY 1 cut(s) 1216
MhlI GDGCHC 3 cut(s) 183, 200, 1423
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 162, 483, 648, 666, 750, 1239, 1479
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 64, 380
MmeI TCCRAC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1255
MroI TCCGGA 1 cut(s) 1115
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 539, 651, 837, 893, 1341, 1482
MslI CAYNNNNRTG 2 cut(s) 468, 920
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 3 cut(s) 201, 779, 1116
MspR9I CCNGG 6 cut(s) 31, 201, 420, 638, 996, 1046
MvaI CCWGG 5 cut(s) 31, 420, 638, 996, 1046
MvnI CGCG 1 cut(s) 598
NciI CCSGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 309
NdeII GATC 5 cut(s) 76, 250, 1216, 1230, 1401
NheI GCTAGC 1 cut(s) 938
NlaIV GGNNCC 6 cut(s) 35, 198, 199, 580, 592, 1169
NmeAIII GCCGAG 2 cut(s) 74, 968
NmuCI GTSAC 3 cut(s) 397, 1040, 1375
NruI TCGCGA 1 cut(s) 598
NsiI ATGCAT 1 cut(s) 1255
NspI RCATGY 5 cut(s) 389, 405, 469, 611, 1355
PaeI GCATGC 2 cut(s) 389, 1355
PciI ACATGT 2 cut(s) 401, 465
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 912, 964, 971
PflFI GACNNNGTC 1 cut(s) 351
PfoI TCCNGGA 2 cut(s) 418, 636
PkrI GCNGC 5 cut(s) 41, 143, 155, 844, 902
PleI GAGTC 2 cut(s) 64, 379
PpsI GAGTC 2 cut(s) 64, 379
PscI ACATGT 2 cut(s) 401, 465
PshAI GACNNNNGTC 1 cut(s) 957
PshBI ATTAAT 2 cut(s) 651, 837
Psp6I CCWGG 5 cut(s) 29, 418, 636, 994, 1044
PspEI GGTNACC 2 cut(s) 1040, 1375
PspGI CCWGG 5 cut(s) 29, 418, 636, 994, 1044
PspN4I GGNNCC 6 cut(s) 35, 198, 199, 580, 592, 1169
PspOMI GGGCCC 1 cut(s) 196
PspPI GGNCC 9 cut(s) 27, 196, 197, 347, 898, 992, 1168, 1334, 1459
PsuI RGATCY 1 cut(s) 1216
PsyI GACNNNGTC 1 cut(s) 351
RruI TCGCGA 1 cut(s) 598
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 2 cut(s) 468, 920
SalI GTCGAC 1 cut(s) 950
SaqAI TTAA 6 cut(s) 539, 651, 837, 893, 1341, 1482
SatI GCNGC 5 cut(s) 40, 142, 154, 843, 901
Sau3AI GATC 5 cut(s) 76, 250, 1216, 1230, 1401
Sau96I GGNCC 9 cut(s) 27, 196, 197, 347, 898, 992, 1168, 1334, 1459
SchI GAGTC 2 cut(s) 64, 380
ScrFI CCNGG 6 cut(s) 31, 201, 420, 638, 996, 1046
SduI GDGCHC 3 cut(s) 183, 200, 1423
SexAI ACCWGGT 2 cut(s) 994, 1044
SfaNI GCATC 3 cut(s) 642, 1099, 1262
SfcI CTRYAG 1 cut(s) 904
SinI GGWCC 4 cut(s) 347, 992, 1168, 1459
SmiMI CAYNNNNRTG 2 cut(s) 468, 920
SmlI CTYRAG 1 cut(s) 1463
SmoI CTYRAG 1 cut(s) 1463
SphI GCATGC 2 cut(s) 389, 1355
Sse9I AATT 7 cut(s) 162, 483, 648, 666, 750, 1239, 1479
SsiI CCGC 5 cut(s) 12, 37, 142, 453, 901
SspI AATATT 1 cut(s) 1162
SspMI CTAG 2 cut(s) 24, 939
StyD4I CCNGG 6 cut(s) 29, 199, 418, 636, 994, 1044
StyI CCWWGG 2 cut(s) 320, 586
TaaI ACNGT 7 cut(s) 433, 908, 956, 1289, 1330, 1425, 1431
TaiI ACGT 3 cut(s) 213, 355, 951
TaqI TCGA 9 cut(s) 45, 216, 296, 337, 369, 765, 804, 951, 1178
TaqII GACCGA 1 cut(s) 1394
TasI AATT 7 cut(s) 162, 483, 648, 666, 750, 1239, 1479
TauI GCSGC 2 cut(s) 144, 903
TfiI GAWTC 3 cut(s) 912, 964, 971
Tru1I TTAA 6 cut(s) 539, 651, 837, 893, 1341, 1482
Tru9I TTAA 6 cut(s) 539, 651, 837, 893, 1341, 1482
TscAI CASTG 7 cut(s) 70, 154, 814, 913, 1294, 1333, 1411
TseFI GTSAC 3 cut(s) 397, 1040, 1375
TseI GCWGC 3 cut(s) 39, 153, 842
Tsp45I GTSAC 3 cut(s) 397, 1040, 1375
TspDTI ATGAA 5 cut(s) 296, 904, 1023, 1112, 1286
TspGWI ACGGA 4 cut(s) 276, 1223, 1353, 1358
TspRI CASTG 7 cut(s) 70, 154, 814, 913, 1294, 1333, 1411
Tth111I GACNNNGTC 1 cut(s) 351
VpaK11BI GGWCC 4 cut(s) 347, 992, 1168, 1459
VspI ATTAAT 2 cut(s) 651, 837
XagI CCTNNNNNAGG 1 cut(s) 1466
XapI RAATTY 2 cut(s) 483, 750
XceI RCATGY 5 cut(s) 389, 405, 469, 611, 1355
XcmI CCANNNNNNNNNTGG 3 cut(s) 154, 572, 1523
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 2 cut(s) 24, 939
ZraI GACGTC 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.