Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000762

Physical Location & Seq

Genomic Coordinates Reverse (-)

18332280 .. 18337418

Gene Structure 5139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1200000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGCCAGCTCCGCTTCCATTTCTAGGCCCAGGTTCCGCTGCTTCGACCCTAGTAACTCTTACCCTGCGACTCCCGATCACCCTCGCTACTTCTCGTCTTCCCCTCCCACCTCCAGCCCCTCCACTCCCCGAGTGAACGGCCGCTCCAGTGGCAGCATGGCAATTTCCGACAACTCCGAGCTCAAAATCGCCACCGGCCCCGGTGGCTACGTCCTCGAAGATGTTCCTCACTTAGTTGATTACATCCCCGATCTCCCCACATATCCCAATCCATTACAAGACAATCCGTCCTATTCGGTTGTTAAGCAGTATTTCGTTAATGCAGATGATACCGTTGCTCAAAAGATTGTTGTTCATAAGGATAGTCCAAGGGGGACACACTTTCGACGCTCTGGACCACGTCAGAAGGTGTATTTCGAGTCAAATGATGTGCATGCGTGCATTGTGACATGTGGTGGTCTCTGTCCAGGACTGAATACAGTTATTAGGGAAATAGTATGCGGTCTGTACCACATGTATGGTGTCAGAAGAATTCTCGGAATAGAGGGTGGATATAGAGGGTTTTATGCCAGAAATACAATCCCCTTAACACCGAAGGTTGTGAATGACATCCATAAACGAGGTGGCACCATCCTTGGAACCTCGCGAGGTGGGCATGTTACCTCAAAGATTGTGGACAGCATCCAGGACAGAGGAATTAATCAGGTTTATGTAATTGGAGGAGATGGAACTCAGAAAGGAGCTTCGGTGATTTTTGAGGAAATCAGGAGGCGTGGCCTCAAAGTAGTGGTGGCAGGAATTCCCAAAACCATCGATAATGACATTCCGGTTATAGACAGGTCTTTTGGTTTTGACACTGCTGTTGAGGAGGCTCAACGAGCCATTAATGCAGCTCATGTTGAAGCAGAAAGCGTTGAGAATGGTATTGGCTTCGTCAAGTTAATGGGCCGCTACAGTGGTTTCATAGCAATGTATGCTACTCTTGGTAGCCGAGACGTCGACTGCTGTCTGATTCCAGAGTCACCTTTTTATCTGGAAGGACCAGGCGGACTTTATGAATACATTGAAAGAAGACTGAAGGAGGATGGTCACCTGGTTATAGTAATGGCTGAAGGTGCAGGACAAGAGCTTCTTGCTGAAAGCATGAACCAACAGGATGCTTCTGGAAATAAGCTTCTCCAAGATGTTGGTCTCTGGTTATCTCAAAATATCAGGGACCACTTCTCGAAACAGAGAAATATGGCTATAAATCTCAAATATATAGATCCTACTTACATGATCCGTGCGATTCCAAGTAATGCATCTGATAATGTATATTGCACCCTCCTTGCTCACAGTGCCATTCATGGTGCGATGGCTGGATACACAGGCTTCACTGTCGGCCCTGTTAACGGGAGGCATGCTTACATTCCGTTCCGTCGGGTGACCGAGAGGCAGAACAAGGTTGTGATCACTGACAGAATGTGGGCACGGTTACTGTCATCAACCAATCAGCCGAGCTTCTTGGACCTGAAAGAGGTCGAAGCAATGAAGAAAGAGGAAAAGGAAGAGGAAACAAAAGCCCAACTGATAGTTGGGGAAAACCATACTGAAGCAACTTCAGCGTCTTTACCGAAGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230

Pfam Domains

Protein Families

PFK

Protein Analysis

541

Amino Acids

59.43

Weight (kDa)

6.95

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	147 - 449	1.6e-60	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000762)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26270 AT4G29220 AT4G32840 AT5G56630
fragaria_vesca	FvH4_1g06570 FvH4_2g35190 FvH4_6g32610 FvH4_6g32610 FvH4_6g32610
malus_domestica	MD02G1068900.v1.1 MD08G1109700.v1.1 MD09G1200000.v1.1 MD15G1089400.v1.1 MD17G1180600.v1.1 MD17G1180700.v1.1 MD17G1180800.v1.1
prunus_persica	Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.1G444000_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.3G056600_v2.0.a1 Prupe.7G216700_v2.0.a1
pyrus_communis	pycom02g05460 pycom07g22220 pycom09g11720 pycom15g08380 pycom17g19000 pycom17g19010 pycom17g19050
rosa_chinensis	RchiOBHm_Chr2g0092311 RchiOBHm_Chr2g0141061 RchiOBHm_Chr6g0306751
rosa_laevigata	RLG00000010768 RLG00000016296 RLG00000019912
rosa_multiflora	Rmu_sc0003289.1_g000020 Rmu_sc0010596.1_g000001 Rmu_sc0025856.1_g000001 Rmu_sc0032226.1_g000001
rosa_roxburghii	Rroxscaffold_2G00104240 Rroxscaffold_2G00149270 Rroxscaffold_4G00313720 Rroxscaffold_7G00161490
rosa_rugosa	Rorug02G0024700 Rorug02G0366600 Rorug02G0366800 Rorug04G0176400 Rorug06G0352400 Rorug06G0352500
rosa_samantha	Rh2AG417900 Rh2BG070100 Rh2BG427800 Rh2CG071300 Rh2CG404200 Rh2DG069100 Rh2DG437400 Rh2DG437800 Rh4CG359100 Rh6AG464500 Rh6BG436300 Rh6CG478800 Rh6DG019000 Rh6DG465900 Rh6DG478600 Rh7DG340800
rosa_wichuraiana	Rw2G006170 Rw2G034210 Rw2G034270 Rw6G040480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	999
AccB1I	GGYRCC	1 cut(s)	626
AccBSI	CCGCTC	1 cut(s)	144
AccI	GTMKAC	1 cut(s)	999
AccII	CGCG	1 cut(s)	646
AciI	CCGC	6 cut(s)	12, 37, 142, 501, 949, 1047
AclWI	GGATC	2 cut(s)	1259, 1273
AcoI	YGGCCR	2 cut(s)	3, 139
AcsI	RAATTY	2 cut(s)	531, 798
AcuI	CTGAAG	4 cut(s)	1097, 1131, 1584, 1611
AcyI	GRCGYC	1 cut(s)	996
AfaI	GTAC	1 cut(s)	509
AfiI	CCNNNNNNNGG	3 cut(s)	24, 1391, 1516
AflIII	ACRYGT	2 cut(s)	449, 513
AgsI	TTSAA	2 cut(s)	902, 1067
AhdI	GACNNNNNGTC	1 cut(s)	286
AjiI	CACGTC	1 cut(s)	401
AjnI	CCWGG	5 cut(s)	29, 466, 684, 1042, 1092
AloI	GAACNNNNNNTCC	2 cut(s)	128, 160
AluBI	AGCT	7 cut(s)	9, 181, 743, 893, 1129, 1174, 1500
AluI	AGCT	7 cut(s)	9, 181, 743, 893, 1129, 1174, 1500
Alw21I	GWGCWC	1 cut(s)	183
Alw26I	GTCTC	3 cut(s)	464, 987, 1196
AlwI	GGATC	2 cut(s)	1259, 1273
Ama87I	CYCGRG	1 cut(s)	129
AoxI	GGCC	7 cut(s)	3, 26, 139, 196, 775, 946, 1381
ApeKI	GCWGC	3 cut(s)	39, 153, 890
ApoI	RAATTY	2 cut(s)	531, 798
ArsI	GACNNNNNNTTYG	2 cut(s)	827, 859
AseI	ATTAAT	2 cut(s)	699, 885
Asp700I	GAANNNNTTC	1 cut(s)	222
AspS9I	GGNCC	8 cut(s)	27, 197, 395, 946, 1040, 1216, 1382, 1507
AsuC2I	CCSGG	1 cut(s)	201
AsuHPI	GGTGA	5 cut(s)	71, 760, 1014, 1082, 1435
AvaI	CYCGRG	1 cut(s)	129
AvaII	GGWCC	4 cut(s)	395, 1040, 1216, 1507
BaeGI	GKGCMC	1 cut(s)	1471
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	626
BanII	GRGCYC	1 cut(s)	183
BbsI	GAAGAC	2 cut(s)	90, 1078
Bbv12I	GWGCWC	1 cut(s)	183
BbvI	GCAGC	3 cut(s)	26, 165, 902
BccI	CCATC	5 cut(s)	638, 719, 818, 1079, 1348
BceAI	ACGGC	1 cut(s)	154
BciT130I	CCWGG	5 cut(s)	31, 468, 686, 1044, 1094
BciVI	GTATCC	1 cut(s)	1355
BclI	TGATCA	1 cut(s)	1449
BcnI	CCSGG	1 cut(s)	201
BcoDI	GTCTC	3 cut(s)	464, 987, 1196
BfaI	CTAG	2 cut(s)	24, 51
BfmI	CTRYAG	1 cut(s)	952
BfuI	GTATCC	1 cut(s)	1355
BglI	GCCNNNNNGGC	1 cut(s)	204
BisI	GCNGC	5 cut(s)	40, 142, 154, 891, 949
BlsI	GCNGC	5 cut(s)	41, 143, 155, 892, 950
Bme1390I	CCNGG	6 cut(s)	31, 201, 468, 686, 1044, 1094
Bme18I	GGWCC	4 cut(s)	395, 1040, 1216, 1507
BmeRI	GACNNNNNGTC	1 cut(s)	286
BmeT110I	CYCGRG	1 cut(s)	129
BmgBI	CACGTC	1 cut(s)	401
BmgT120I	GGNCC	8 cut(s)	27, 197, 395, 946, 1040, 1216, 1382, 1507
BmiI	GGNNCC	5 cut(s)	35, 199, 628, 640, 1217
BmrFI	CCNGG	6 cut(s)	31, 201, 468, 686, 1044, 1094
BmsI	GCATC	3 cut(s)	690, 1147, 1310
BoxI	GACNNNNGTC	1 cut(s)	1005
BpiI	GAAGAC	2 cut(s)	90, 1078
BpmI	CTGGAG	2 cut(s)	97, 130
BpuMI	CCSGG	1 cut(s)	201
Bsa29I	ATCGAT	1 cut(s)	813
BsaHI	GRCGYC	1 cut(s)	996
BsaI	GGTCTC	2 cut(s)	464, 1196
BsaJI	CCNNGG	4 cut(s)	29, 199, 368, 634
BsaWI	WCCGGW	1 cut(s)	826
BsaXI	ACNNNNNCTCC	4 cut(s)	128, 158, 732, 762
Bsc4I	CCNNNNNNNGG	3 cut(s)	24, 1391, 1516
Bse118I	RCCGGY	1 cut(s)	194
Bse1I	ACTGG	1 cut(s)	147
Bse3DI	GCAATG	2 cut(s)	975, 1533
BseBI	CCWGG	5 cut(s)	31, 468, 686, 1044, 1094
BseCI	ATCGAT	1 cut(s)	813
BseDI	CCNNGG	4 cut(s)	29, 199, 368, 634
BseGI	GGATG	6 cut(s)	243, 609, 630, 681, 1090, 1162
BseLI	CCNNNNNNNGG	3 cut(s)	24, 1391, 1516
BseMI	GCAATG	2 cut(s)	975, 1533
BseMII	CTCAG	1 cut(s)	746
BseNI	ACTGG	1 cut(s)	147
BseRI	GAGGAG	2 cut(s)	735, 881
BseSI	GKGCMC	1 cut(s)	1471
BseX3I	CGGCCG	1 cut(s)	139
BseXI	GCAGC	3 cut(s)	26, 165, 902
BsgI	GTGCAG	1 cut(s)	1137
Bsh1236I	CGCG	1 cut(s)	646
Bsh1285I	CGRYCG	1 cut(s)	142
BshFI	GGCC	7 cut(s)	5, 28, 141, 198, 777, 948, 1383
BshNI	GGYRCC	1 cut(s)	626
BshVI	ATCGAT	1 cut(s)	813
BsiEI	CGRYCG	1 cut(s)	142
BsiHKAI	GWGCWC	1 cut(s)	183
BsiHKCI	CYCGRG	1 cut(s)	129
BsiSI	CCGG	3 cut(s)	195, 201, 827
BslFI	GGGAC	2 cut(s)	388, 1229
BslI	CCNNNNNNNGG	3 cut(s)	24, 1391, 1516
BsmAI	GTCTC	3 cut(s)	464, 987, 1196
BsmBI	CGTCTC	1 cut(s)	987
BsmFI	GGGAC	2 cut(s)	388, 1229
BsnI	GGCC	7 cut(s)	5, 28, 141, 198, 777, 948, 1383
Bso31I	GGTCTC	2 cut(s)	464, 1196
BsoBI	CYCGRG	1 cut(s)	129
Bsp1286I	GDGCHC	2 cut(s)	183, 1471
Bsp143I	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
Bsp68I	TCGCGA	1 cut(s)	646
BspACI	CCGC	6 cut(s)	12, 37, 142, 501, 949, 1047
BspANI	GGCC	7 cut(s)	5, 28, 141, 198, 777, 948, 1383
BspCNI	CTCAG	1 cut(s)	745
BspDI	ATCGAT	1 cut(s)	813
BspFNI	CGCG	1 cut(s)	646
BspLI	GGNNCC	5 cut(s)	35, 199, 628, 640, 1217
BspPI	GGATC	2 cut(s)	1259, 1273
BspT107I	GGYRCC	1 cut(s)	626
BspTNI	GGTCTC	2 cut(s)	464, 1196
BsrBI	CCGCTC	1 cut(s)	144
BsrDI	GCAATG	2 cut(s)	975, 1533
BsrFI	RCCGGY	1 cut(s)	194
BsrI	ACTGG	1 cut(s)	147
BssAI	RCCGGY	1 cut(s)	194
BssECI	CCNNGG	4 cut(s)	29, 199, 368, 634
BssMI	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
BssNI	GRCGYC	1 cut(s)	996
BssT1I	CCWWGG	2 cut(s)	368, 634
Bst2UI	CCWGG	5 cut(s)	31, 468, 686, 1044, 1094
Bst4CI	ACNGT	7 cut(s)	334, 481, 956, 1337, 1378, 1473, 1479
Bst6I	CTCTTC	1 cut(s)	1542
BstACI	GRCGYC	1 cut(s)	996
BstAPI	GCANNNNNTGC	1 cut(s)	974
BstC8I	GCNNGC	4 cut(s)	7, 435, 439, 1401
BstDEI	CTNAG	2 cut(s)	232, 732
BstEII	GGTNACC	2 cut(s)	1088, 1423
BstENI	CCTNNNNNAGG	1 cut(s)	1514
BstF5I	GGATG	6 cut(s)	243, 609, 630, 681, 1090, 1162
BstFNI	CGCG	1 cut(s)	646
BstKTI	GATC	5 cut(s)	79, 253, 1267, 1281, 1452
BstMAI	GTCTC	3 cut(s)	464, 987, 1196
BstMBI	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
BstMCI	CGRYCG	1 cut(s)	142
BstNI	CCWGG	5 cut(s)	31, 468, 686, 1044, 1094
BstNSI	RCATGY	5 cut(s)	437, 453, 517, 659, 1403
BstPAI	GACNNNNGTC	1 cut(s)	1005
BstPI	GGTNACC	2 cut(s)	1088, 1423
BstSCI	CCNGG	6 cut(s)	29, 199, 466, 684, 1042, 1092
BstSFI	CTRYAG	1 cut(s)	952
BstSLI	GKGCMC	1 cut(s)	1471
BstUI	CGCG	1 cut(s)	646
BstV1I	GCAGC	3 cut(s)	26, 165, 902
BstV2I	GAAGAC	2 cut(s)	90, 1078
BstX2I	RGATCY	1 cut(s)	1264
BstXI	CCANNNNNNTGG	2 cut(s)	518, 1187
BstYI	RGATCY	1 cut(s)	1264
BstZI	CGGCCG	1 cut(s)	139
Bsu15I	ATCGAT	1 cut(s)	813
BsuI	GTATCC	1 cut(s)	1355
BsuRI	GGCC	7 cut(s)	5, 28, 141, 198, 777, 948, 1383
BsuTUI	ATCGAT	1 cut(s)	813
BtgZI	GCGATG	1 cut(s)	1367
BtrI	CACGTC	1 cut(s)	401
BtsCI	GGATG	6 cut(s)	243, 609, 630, 681, 1090, 1162
BtsI	GCAGTG	1 cut(s)	855
BtsIMutI	CAGTG	6 cut(s)	154, 855, 961, 1342, 1374, 1452
BtuMI	TCGCGA	1 cut(s)	646
Cac8I	GCNNGC	4 cut(s)	7, 435, 439, 1401
Cfr10I	RCCGGY	1 cut(s)	194
Cfr13I	GGNCC	8 cut(s)	27, 197, 395, 946, 1040, 1216, 1382, 1507
ClaI	ATCGAT	1 cut(s)	813
CseI	GACGC	2 cut(s)	396, 1593
CsiI	ACCWGGT	1 cut(s)	1092
Csp6I	GTAC	1 cut(s)	508
CspCI	CAANNNNNGTGG	2 cut(s)	654, 689
CviQI	GTAC	1 cut(s)	508
DdeI	CTNAG	2 cut(s)	232, 732
DpnI	GATC	5 cut(s)	78, 252, 1266, 1280, 1451
DpnII	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
DriI	GACNNNNNGTC	1 cut(s)	286
EaeI	YGGCCR	2 cut(s)	3, 139
EagI	CGGCCG	1 cut(s)	139
Eam1104I	CTCTTC	1 cut(s)	1542
Eam1105I	GACNNNNNGTC	1 cut(s)	286
EarI	CTCTTC	1 cut(s)	1542
EciI	GGCGGA	1 cut(s)	1062
Ecl136II	GAGCTC	1 cut(s)	181
EclXI	CGGCCG	1 cut(s)	139
Eco130I	CCWWGG	2 cut(s)	368, 634
Eco24I	GRGCYC	1 cut(s)	183
Eco31I	GGTCTC	2 cut(s)	464, 1196
Eco47I	GGWCC	4 cut(s)	395, 1040, 1216, 1507
Eco52I	CGGCCG	1 cut(s)	139
Eco53kI	GAGCTC	1 cut(s)	181
Eco57I	CTGAAG	4 cut(s)	1097, 1131, 1584, 1611
Eco88I	CYCGRG	1 cut(s)	129
Eco91I	GGTNACC	2 cut(s)	1088, 1423
EcoICRI	GAGCTC	1 cut(s)	181
EcoNI	CCTNNNNNAGG	1 cut(s)	1514
EcoO65I	GGTNACC	2 cut(s)	1088, 1423
EcoRI	GAATTC	2 cut(s)	531, 798
EcoRII	CCWGG	5 cut(s)	29, 466, 684, 1042, 1092
EcoT14I	CCWWGG	2 cut(s)	368, 634
EcoT22I	ATGCAT	1 cut(s)	1303
EcoT38I	GRGCYC	1 cut(s)	183
ErhI	CCWWGG	2 cut(s)	368, 634
Esp3I	CGTCTC	1 cut(s)	987
FalI	AAGNNNNNCTT	2 cut(s)	1116, 1148
FaqI	GGGAC	2 cut(s)	388, 1229
FbaI	TGATCA	1 cut(s)	1449
FblI	GTMKAC	1 cut(s)	999
Fnu4HI	GCNGC	5 cut(s)	40, 142, 154, 891, 949
FokI	GGATG	6 cut(s)	230, 596, 617, 668, 1097, 1169
FriOI	GRGCYC	1 cut(s)	183
Fsp4HI	GCNGC	5 cut(s)	40, 142, 154, 891, 949
FspBI	CTAG	2 cut(s)	24, 51
GluI	GCNGC	5 cut(s)	40, 142, 154, 891, 949
GsuI	CTGGAG	2 cut(s)	97, 130
HaeIII	GGCC	7 cut(s)	5, 28, 141, 198, 777, 948, 1383
HapII	CCGG	3 cut(s)	195, 201, 827
HgaI	GACGC	2 cut(s)	396, 1593
Hin1I	GRCGYC	1 cut(s)	996
HincII	GTYRAC	2 cut(s)	1000, 1390
HindII	GTYRAC	2 cut(s)	1000, 1390
HindIII	AAGCTT	1 cut(s)	1172
HinfI	GANTC	5 cut(s)	70, 419, 1012, 1019, 1288
HpaI	GTTAAC	1 cut(s)	1390
HpaII	CCGG	3 cut(s)	195, 201, 827
HphI	GGTGA	5 cut(s)	71, 760, 1014, 1082, 1435
Hpy166II	GTNNAC	4 cut(s)	136, 676, 1000, 1390
Hpy188I	TCNGA	8 cut(s)	169, 178, 405, 527, 539, 735, 1011, 1306
Hpy188III	TCNNGA	8 cut(s)	74, 393, 645, 766, 1016, 1034, 1164, 1225
Hpy8I	GTNNAC	4 cut(s)	136, 676, 1000, 1390
Hpy99I	CGWCG	3 cut(s)	390, 1001, 1422
HpyAV	CCTTC	6 cut(s)	400, 589, 1031, 1072, 1106, 1609
HpyCH4III	ACNGT	7 cut(s)	334, 481, 956, 1337, 1378, 1473, 1479
HpyCH4IV	ACGT	3 cut(s)	210, 400, 996
HpyCH4V	TGCA	7 cut(s)	323, 433, 441, 890, 1118, 1301, 1320
HpyF3I	CTNAG	2 cut(s)	232, 732
HpySE526I	ACGT	3 cut(s)	210, 400, 996
Hsp92I	GRCGYC	1 cut(s)	996
Ksp22I	TGATCA	1 cut(s)	1449
KspAI	GTTAAC	1 cut(s)	1390
Kzo9I	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
LmnI	GCTCC	3 cut(s)	14, 149, 740
Lsp1109I	GCAGC	3 cut(s)	26, 165, 902
LweI	GCATC	3 cut(s)	690, 1147, 1310
MabI	ACCWGGT	1 cut(s)	1092
MaeI	CTAG	2 cut(s)	24, 51
MaeII	ACGT	3 cut(s)	210, 400, 996
MaeIII	GTNAC	7 cut(s)	53, 445, 658, 1020, 1088, 1423, 1473
MalI	GATC	5 cut(s)	78, 252, 1266, 1280, 1451
MbiI	CCGCTC	1 cut(s)	144
MboI	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
MboII	GAAGA	6 cut(s)	90, 230, 540, 1083, 1543, 1559
MflI	RGATCY	1 cut(s)	1264
MhlI	GDGCHC	2 cut(s)	183, 1471
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	5 cut(s)	162, 531, 696, 714, 798
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	64, 428, 1028
MmeI	TCCRAC	1 cut(s)	192
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	1303
MroXI	GAANNNNTTC	1 cut(s)	222
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	303, 318, 587, 699, 885, 941, 1389
MslI	CAYNNNNRTG	2 cut(s)	516, 968
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	39
MspI	CCGG	3 cut(s)	195, 201, 827
MspR9I	CCNGG	6 cut(s)	31, 201, 468, 686, 1044, 1094
MvaI	CCWGG	5 cut(s)	31, 468, 686, 1044, 1094
MvnI	CGCG	1 cut(s)	646
NciI	CCSGG	1 cut(s)	201
NdeII	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
NlaIV	GGNNCC	5 cut(s)	35, 199, 628, 640, 1217
NmeAIII	GCCGAG	2 cut(s)	1016, 1521
NmuCI	GTSAC	4 cut(s)	445, 1020, 1088, 1423
NruI	TCGCGA	1 cut(s)	646
NsiI	ATGCAT	1 cut(s)	1303
NspI	RCATGY	5 cut(s)	437, 453, 517, 659, 1403
PaeI	GCATGC	2 cut(s)	437, 1403
PciI	ACATGT	2 cut(s)	449, 513
PdmI	GAANNNNTTC	1 cut(s)	222
PfeI	GAWTC	2 cut(s)	1012, 1288
PflFI	GACNNNGTC	1 cut(s)	399
PfoI	TCCNGGA	2 cut(s)	466, 684
PkrI	GCNGC	5 cut(s)	41, 143, 155, 892, 950
PleI	GAGTC	3 cut(s)	64, 427, 1027
PpsI	GAGTC	3 cut(s)	64, 427, 1027
PscI	ACATGT	2 cut(s)	449, 513
PshAI	GACNNNNGTC	1 cut(s)	1005
PshBI	ATTAAT	2 cut(s)	699, 885
Psp124BI	GAGCTC	1 cut(s)	183
Psp6I	CCWGG	5 cut(s)	29, 466, 684, 1042, 1092
PspEI	GGTNACC	2 cut(s)	1088, 1423
PspGI	CCWGG	5 cut(s)	29, 466, 684, 1042, 1092
PspN4I	GGNNCC	5 cut(s)	35, 199, 628, 640, 1217
PspPI	GGNCC	8 cut(s)	27, 197, 395, 946, 1040, 1216, 1382, 1507
PsuI	RGATCY	1 cut(s)	1264
PsyI	GACNNNGTC	1 cut(s)	399
RruI	TCGCGA	1 cut(s)	646
RsaI	GTAC	1 cut(s)	509
RsaNI	GTAC	1 cut(s)	508
RseI	CAYNNNNRTG	2 cut(s)	516, 968
SacI	GAGCTC	1 cut(s)	183
SalI	GTCGAC	1 cut(s)	998
SaqAI	TTAA	7 cut(s)	303, 318, 587, 699, 885, 941, 1389
SatI	GCNGC	5 cut(s)	40, 142, 154, 891, 949
Sau3AI	GATC	5 cut(s)	76, 250, 1264, 1278, 1449
Sau96I	GGNCC	8 cut(s)	27, 197, 395, 946, 1040, 1216, 1382, 1507
SchI	GAGTC	3 cut(s)	64, 428, 1028
ScrFI	CCNGG	6 cut(s)	31, 201, 468, 686, 1044, 1094
SduI	GDGCHC	2 cut(s)	183, 1471
SexAI	ACCWGGT	1 cut(s)	1092
SfaNI	GCATC	3 cut(s)	690, 1147, 1310
SfcI	CTRYAG	1 cut(s)	952
SinI	GGWCC	4 cut(s)	395, 1040, 1216, 1507
SmiMI	CAYNNNNRTG	2 cut(s)	516, 968
SphI	GCATGC	2 cut(s)	437, 1403
Sse9I	AATT	5 cut(s)	162, 531, 696, 714, 798
SsiI	CCGC	6 cut(s)	12, 37, 142, 501, 949, 1047
SspMI	CTAG	2 cut(s)	24, 51
SstI	GAGCTC	1 cut(s)	183
StyD4I	CCNGG	6 cut(s)	29, 199, 466, 684, 1042, 1092
StyI	CCWWGG	2 cut(s)	368, 634
TaaI	ACNGT	7 cut(s)	334, 481, 956, 1337, 1378, 1473, 1479
TaiI	ACGT	3 cut(s)	213, 403, 999
TaqI	TCGA	8 cut(s)	45, 216, 385, 417, 813, 999, 1226, 1521
TaqII	GACCGA	1 cut(s)	1442
TasI	AATT	5 cut(s)	162, 531, 696, 714, 798
TauI	GCSGC	2 cut(s)	144, 951
TfiI	GAWTC	2 cut(s)	1012, 1288
Tru1I	TTAA	7 cut(s)	303, 318, 587, 699, 885, 941, 1389
Tru9I	TTAA	7 cut(s)	303, 318, 587, 699, 885, 941, 1389
TscAI	CASTG	6 cut(s)	154, 862, 961, 1342, 1381, 1459
TseFI	GTSAC	4 cut(s)	445, 1020, 1088, 1423
TseI	GCWGC	3 cut(s)	39, 153, 890
Tsp45I	GTSAC	4 cut(s)	445, 1020, 1088, 1423
TspDTI	ATGAA	6 cut(s)	344, 952, 1071, 1160, 1334, 1544
TspGWI	ACGGA	4 cut(s)	276, 1271, 1401, 1406
TspRI	CASTG	6 cut(s)	154, 862, 961, 1342, 1381, 1459
Tth111I	GACNNNGTC	1 cut(s)	399
VpaK11BI	GGWCC	4 cut(s)	395, 1040, 1216, 1507
VspI	ATTAAT	2 cut(s)	699, 885
XagI	CCTNNNNNAGG	1 cut(s)	1514
XapI	RAATTY	2 cut(s)	531, 798
XceI	RCATGY	5 cut(s)	437, 453, 517, 659, 1403
XcmI	CCANNNNNNNNNTGG	3 cut(s)	154, 620, 1571
XmiI	GTMKAC	1 cut(s)	999
XmnI	GAANNNNTTC	1 cut(s)	222
XspI	CTAG	2 cut(s)	24, 51
ZraI	GACGTC	1 cut(s)	997
Zsp2I	ATGCAT	1 cut(s)	1303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.