Rroxscaffold_7G00161490

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000762
Physical Location & Seq
Reverse (-)
4225974 .. 4230103
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161490.1

Sequence Viewer

Length: 1509 bp
ATGGCGGTTTCCGGTGATCTGAAGATGAAGCTCGTCAAAGGCGATGCCGGTTACGTCCTCGAAGATGTGCCTCATATCGCCGATTACTTGTCCGATCTTCCTACCTATCCCAATCCACTGCAAATCAATCCTGCTTACTCTGTAGTCAAGTACGAACTACTTTTCCAGAACAGAACAATGATCTATGCTTTTGGTTTGATTCGTTTCCAGCAATATTTTGTTAACCAAGATGACACTGTTGCTCAAAAGATTGTTGTGCACAAGGATAGTCCAAGAGGGACACACCTTCGGCGAGCAGGACCACGCCAAAAGGTGTATTTTGAGGCGGATGAAGTGCATGCCTGTATTGTGACTTGTGGTGGTTTGTGCCCTGGGCTCAACACAGTGATCAGGGAAATTGTCTGTGGACTTTATCACATGTATGGTGTCACCAGGGTTCTTGGAATAGATGGAGGATATAGTGGTTTCTATTCCCGAAATACTATTGAGTTGACACCTAGGGTTGTGAATGACATCCACAAACGTGGTGGTACTATCCTTGGGACATCACGAGGAGGCTATGATACCTCGAAGATAGTTGACAGCATTCATGATCGTGGAATTAACCATGTTTACATAATTGGAGGTGATGGAACTCAAAAAGGAGCATCTGTGATATTTGAGGAAATTAGACGCCGTGGCCTCAAAGTTTCTGTTGTAGGAGTTCCTAAAACAATAGACAATGACATACCGGTTATAGATAGATCCTTTGGCTTTGATACTGCAGTTGAGGAGGCCCAGCGTGCCATATGTGCAGCTCATGTGGAAGCTGAAAGTATTGAGCATGGTATTGGTCTTGTGAAGCTAATGGGACGCTACAGCGGTTTCATAGCTATGTATGCTACACTTGCCAGTCGAGATGTGGACTGTTGTTTGATTCCAGAGTCCCCCTTCTTTCTTGAAGGACGAGGTGGACTTTTTGAGTTCATTGAAAAACGACTCAAAGAAAATGGACATATGGTTATTGTGATTGCTGAGGGAGCAGGACAGGATCTTCTTTCAGAGAGCTTGCAATCCATGAACCAGCAAGATGCTTCAGGAAACAAGCTACTTCAAGATGTTGGGTTATGGATATCTCAGAGGATCAAGGATCACTTTGTGAGACAACAAAAGATGTCTATTAATCTGAAATATATAGATCCTACATACATGATCAGGGCTATTCCAGGTAATGCAGCTGACAATGTCTACTGCACACTGCTGGCTCAAAGTGCCGTTCATGGAGCAATGGCAGGGTTCACGGGCTTTGTAGTTGGGCCTGTCAATGGAAGACATTCTTACATTCCTTTTAATCATATTACTGATAGACAGAACAAGGTTGTGATTACCGATAGGATGTGGGCACGACTCCTTTCTTCAACCAACCAGCCGAGCTTCTTGAACCACAAAGACGTTGATGAAGTCCAAAAAGTGGAAGAGCCTCCAACTCAACTGCTAGAAGGGGAAAATTGTGAAGATAGCAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

502

Amino Acids

55.74

Weight (kDa)

6.25

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 115 - 420 9.9e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1450
AccI GTMKAC 1 cut(s) 1227
AciI CCGC 3 cut(s) 5, 326, 861
AclWI GGATC 5 cut(s) 738, 1038, 1130, 1137, 1172
AcuI CTGAAG 2 cut(s) 41, 1059
AcyI GRCGYC 1 cut(s) 673
AdeI CACNNNGTG 1 cut(s) 1138
AfaI GTAC 3 cut(s) 152, 532, 1505
AfiI CCNNNNNNNGG 2 cut(s) 1304, 1450
AflIII ACRYGT 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 730
AgsI TTSAA 5 cut(s) 941, 971, 1094, 1398, 1420
AjnI CCWGG 3 cut(s) 370, 431, 1204
AleI CACNNNNGTG 1 cut(s) 522
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 9 cut(s) 31, 797, 809, 844, 872, 1047, 1087, 1217, 1413
AluI AGCT 9 cut(s) 31, 797, 809, 844, 872, 1047, 1087, 1217, 1413
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 1135
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 5 cut(s) 738, 1038, 1130, 1137, 1172
AoxI GGCC 3 cut(s) 679, 774, 1295
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 794, 1214
AseI ATTAAT 1 cut(s) 1161
AsiGI ACCGGT 1 cut(s) 730
Asp700I GAANNNNTTC 1 cut(s) 1312
AspA2I CCTAGG 1 cut(s) 497
AspS9I GGNCC 3 cut(s) 299, 775, 1295
AsuHPI GGTGA 3 cut(s) 26, 421, 638
AvaII GGWCC 1 cut(s) 299
AvrII CCTAGG 1 cut(s) 497
BaeGI GKGCMC 3 cut(s) 261, 371, 1384
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BanII GRGCYC 1 cut(s) 378
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 1315
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 2 cut(s) 806, 1226
BccI CCATC 2 cut(s) 443, 623
BceAI ACGGC 2 cut(s) 660, 1238
BciT130I CCWGG 3 cut(s) 372, 433, 1206
BclI TGATCA 2 cut(s) 387, 1191
BcoDI GTCTC 1 cut(s) 1135
BfaI CTAG 2 cut(s) 498, 1475
BfmI CTRYAG 3 cut(s) 141, 762, 856
BisI GCNGC 2 cut(s) 795, 1215
BlnI CCTAGG 1 cut(s) 497
BlsI GCNGC 2 cut(s) 796, 1216
BmcAI AGTACT 1 cut(s) 1505
Bme1390I CCNGG 3 cut(s) 372, 433, 1206
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 3 cut(s) 299, 775, 1295
BmrFI CCNGG 3 cut(s) 372, 433, 1206
BmsI GCATC 3 cut(s) 34, 656, 1060
BpiI GAAGAC 1 cut(s) 1315
Bpu10I CCTNAGC 1 cut(s) 1014
BsaHI GRCGYC 1 cut(s) 673
BsaJI CCNNGG 6 cut(s) 370, 371, 432, 497, 538, 676
BsaWI WCCGGW 2 cut(s) 11, 730
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 1304, 1450
Bse118I RCCGGY 2 cut(s) 47, 730
Bse1I ACTGG 1 cut(s) 891
Bse3DI GCAATG 1 cut(s) 1272
BseBI CCWGG 3 cut(s) 372, 433, 1206
BseDI CCNNGG 6 cut(s) 370, 371, 432, 497, 538, 676
BseGI GGATG 3 cut(s) 334, 513, 1380
BseLI CCNNNNNNNGG 2 cut(s) 1304, 1450
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 2 cut(s) 1005, 1130
BseNI ACTGG 1 cut(s) 891
BseRI GAGGAG 2 cut(s) 567, 785
BseSI GKGCMC 3 cut(s) 261, 371, 1384
BseXI GCAGC 2 cut(s) 806, 1226
BseYI CCCAGC 1 cut(s) 777
BsgI GTGCAG 2 cut(s) 813, 1216
BshFI GGCC 3 cut(s) 681, 776, 1297
BshTI ACCGGT 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 3 cut(s) 12, 48, 731
BslFI GGGAC 4 cut(s) 292, 556, 864, 910
BslI CCNNNNNNNGG 2 cut(s) 1304, 1450
BsmAI GTCTC 1 cut(s) 1135
BsmFI GGGAC 4 cut(s) 292, 556, 864, 910
BsmI GAATGC 1 cut(s) 585
BsnI GGCC 3 cut(s) 681, 776, 1297
Bsp1286I GDGCHC 4 cut(s) 261, 371, 378, 1384
BspACI CCGC 3 cut(s) 5, 326, 861
BspANI GGCC 3 cut(s) 681, 776, 1297
BspCNI CTCAG 2 cut(s) 1006, 1129
BspHI TCATGA 1 cut(s) 589
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 5 cut(s) 738, 1038, 1130, 1137, 1172
BspQI GCTCTTC 1 cut(s) 1449
BsrDI GCAATG 1 cut(s) 1272
BsrFI RCCGGY 2 cut(s) 47, 730
BsrI ACTGG 1 cut(s) 891
BssAI RCCGGY 2 cut(s) 47, 730
BssECI CCNNGG 6 cut(s) 370, 371, 432, 497, 538, 676
BssNI GRCGYC 1 cut(s) 673
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 497, 538
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 372, 433, 1206
Bst4CI ACNGT 3 cut(s) 238, 385, 908
Bst6I CTCTTC 1 cut(s) 1449
BstACI GRCGYC 1 cut(s) 673
BstC8I GCNNGC 5 cut(s) 294, 339, 783, 1049, 1242
BstDEI CTNAG 2 cut(s) 1014, 1116
BstDSI CCRYGG 1 cut(s) 676
BstF5I GGATG 3 cut(s) 334, 513, 1380
BstMAI GTCTC 1 cut(s) 1135
BstMWI GCNNNNNNNGC 6 cut(s) 782, 791, 878, 887, 1019, 1250
BstNI CCWGG 3 cut(s) 372, 433, 1206
BstNSI RCATGY 2 cut(s) 341, 421
BstSCI CCNGG 3 cut(s) 370, 431, 1204
BstSFI CTRYAG 3 cut(s) 141, 762, 856
BstSLI GKGCMC 3 cut(s) 261, 371, 1384
BstV1I GCAGC 2 cut(s) 806, 1226
BstV2I GAAGAC 1 cut(s) 1315
BstX2I RGATCY 3 cut(s) 743, 1030, 1177
BstXI CCANNNNNNTGG 1 cut(s) 524
BstYI RGATCY 3 cut(s) 743, 1030, 1177
BsuRI GGCC 3 cut(s) 681, 776, 1297
BtgI CCRYGG 1 cut(s) 676
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 334, 513, 1380
BtsI GCAGTG 2 cut(s) 116, 1235
BtsIMutI CAGTG 4 cut(s) 116, 234, 390, 1235
Cac8I GCNNGC 5 cut(s) 294, 339, 783, 1049, 1242
CciI TCATGA 1 cut(s) 589
Cfr10I RCCGGY 2 cut(s) 47, 730
Cfr13I GGNCC 3 cut(s) 299, 775, 1295
CseI GACGC 2 cut(s) 681, 861
Csp6I GTAC 3 cut(s) 151, 531, 1504
CspAI ACCGGT 1 cut(s) 730
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviAII CATG 9 cut(s) 338, 418, 590, 608, 800, 824, 1057, 1189, 1259
CviQI GTAC 3 cut(s) 151, 531, 1504
DdeI CTNAG 2 cut(s) 1014, 1116
DraIII CACNNNGTG 1 cut(s) 1138
Eam1104I CTCTTC 1 cut(s) 1449
EarI CTCTTC 1 cut(s) 1449
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 2 cut(s) 497, 538
Eco24I GRGCYC 1 cut(s) 378
Eco32I GATATC 1 cut(s) 1113
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 41, 1059
EcoRII CCWGG 3 cut(s) 370, 431, 1204
EcoRV GATATC 1 cut(s) 1113
EcoT14I CCWWGG 2 cut(s) 497, 538
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 497, 538
FaeI CATG 9 cut(s) 341, 421, 593, 611, 803, 827, 1060, 1192, 1262
FalI AAGNNNNNCTT 4 cut(s) 1118, 1150, 1300, 1332
FaqI GGGAC 4 cut(s) 292, 556, 864, 910
FatI CATG 9 cut(s) 337, 417, 589, 607, 799, 823, 1056, 1188, 1258
FauNDI CATATG 2 cut(s) 788, 996
FbaI TGATCA 2 cut(s) 387, 1191
FblI GTMKAC 1 cut(s) 1227
Fnu4HI GCNGC 2 cut(s) 795, 1215
FokI GGATG 3 cut(s) 341, 500, 1387
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 2 cut(s) 795, 1215
FspBI CTAG 2 cut(s) 498, 1475
GluI GCNGC 2 cut(s) 795, 1215
GsaI CCCAGC 1 cut(s) 781
HaeIII GGCC 3 cut(s) 681, 776, 1297
HapII CCGG 3 cut(s) 12, 48, 731
HgaI GACGC 2 cut(s) 681, 861
Hin1I GRCGYC 1 cut(s) 673
Hin1II CATG 9 cut(s) 341, 421, 593, 611, 803, 827, 1060, 1192, 1262
HincII GTYRAC 3 cut(s) 223, 492, 580
HindII GTYRAC 3 cut(s) 223, 492, 580
HinfI GANTC 5 cut(s) 199, 916, 923, 978, 1386
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 3 cut(s) 12, 48, 731
HphI GGTGA 3 cut(s) 26, 421, 638
Hpy188I TCNGA 5 cut(s) 21, 94, 1042, 1119, 1167
HpyAV CCTTC 4 cut(s) 296, 935, 940, 1472
HpyCH4III ACNGT 3 cut(s) 238, 385, 908
HpyCH4IV ACGT 3 cut(s) 54, 523, 1431
HpyCH4V TGCA 8 cut(s) 121, 259, 337, 764, 794, 1051, 1214, 1233
HpyF10VI GCNNNNNNNGC 6 cut(s) 782, 791, 878, 887, 1019, 1250
HpyF3I CTNAG 2 cut(s) 1014, 1116
HpySE526I ACGT 3 cut(s) 54, 523, 1431
Hsp92I GRCGYC 1 cut(s) 673
Hsp92II CATG 9 cut(s) 341, 421, 593, 611, 803, 827, 1060, 1192, 1262
Ksp22I TGATCA 2 cut(s) 387, 1191
KspAI GTTAAC 1 cut(s) 223
LguI GCTCTTC 1 cut(s) 1449
LmnI GCTCC 3 cut(s) 644, 1019, 1262
Lsp1109I GCAGC 2 cut(s) 806, 1226
LweI GCATC 3 cut(s) 34, 656, 1060
MaeI CTAG 2 cut(s) 498, 1475
MaeII ACGT 3 cut(s) 54, 523, 1431
MaeIII GTNAC 3 cut(s) 50, 349, 427
MboII GAAGA 9 cut(s) 34, 74, 89, 583, 1025, 1320, 1386, 1466, 1505
MflI RGATCY 3 cut(s) 743, 1030, 1177
MhlI GDGCHC 4 cut(s) 261, 371, 378, 1384
MluCI AATT 5 cut(s) 396, 600, 618, 666, 1486
MlyI GAGTC 3 cut(s) 932, 972, 1380
MmeI TCCRAC 1 cut(s) 1487
MroXI GAANNNNTTC 1 cut(s) 1312
MseI TTAA 4 cut(s) 222, 603, 1161, 1329
MslI CAYNNNNRTG 4 cut(s) 420, 522, 594, 872
MspA1I CMGCKG 2 cut(s) 861, 1217
MspI CCGG 3 cut(s) 12, 48, 731
MspR9I CCNGG 3 cut(s) 372, 433, 1206
Mva1269I GAATGC 1 cut(s) 585
MvaI CCWGG 3 cut(s) 372, 433, 1206
MwoI GCNNNNNNNGC 6 cut(s) 782, 791, 878, 887, 1019, 1250
NdeI CATATG 2 cut(s) 788, 996
NlaIII CATG 9 cut(s) 341, 421, 593, 611, 803, 827, 1060, 1192, 1262
NmeAIII GCCGAG 1 cut(s) 1434
NmuCI GTSAC 2 cut(s) 349, 427
NspI RCATGY 2 cut(s) 341, 421
OliI CACNNNNGTG 1 cut(s) 522
PaeI GCATGC 1 cut(s) 341
PagI TCATGA 1 cut(s) 589
PasI CCCWGGG 1 cut(s) 371
PciI ACATGT 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 1449
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 1312
PfeI GAWTC 2 cut(s) 199, 916
PflFI GACNNNGTC 1 cut(s) 1223
PflMI CCANNNNNTGG 1 cut(s) 1450
PinAI ACCGGT 1 cut(s) 730
PkrI GCNGC 2 cut(s) 796, 1216
PleI GAGTC 3 cut(s) 931, 972, 1380
PpsI GAGTC 3 cut(s) 931, 972, 1380
PscI ACATGT 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 1161
Psp6I CCWGG 3 cut(s) 370, 431, 1204
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 3 cut(s) 370, 431, 1204
PspPI GGNCC 3 cut(s) 299, 775, 1295
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 3 cut(s) 743, 1030, 1177
PsyI GACNNNGTC 1 cut(s) 1223
PvuII CAGCTG 1 cut(s) 1217
RsaI GTAC 3 cut(s) 152, 532, 1505
RsaNI GTAC 3 cut(s) 151, 531, 1504
RseI CAYNNNNRTG 4 cut(s) 420, 522, 594, 872
SapI GCTCTTC 1 cut(s) 1449
SaqAI TTAA 4 cut(s) 222, 603, 1161, 1329
SatI GCNGC 2 cut(s) 795, 1215
Sau96I GGNCC 3 cut(s) 299, 775, 1295
ScaI AGTACT 1 cut(s) 1505
SchI GAGTC 3 cut(s) 932, 972, 1380
ScrFI CCNGG 3 cut(s) 372, 433, 1206
SduI GDGCHC 4 cut(s) 261, 371, 378, 1384
SfaNI GCATC 3 cut(s) 34, 656, 1060
SfcI CTRYAG 3 cut(s) 141, 762, 856
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 4 cut(s) 420, 522, 594, 872
SphI GCATGC 1 cut(s) 341
Sse9I AATT 5 cut(s) 396, 600, 618, 666, 1486
SsiI CCGC 3 cut(s) 5, 326, 861
SspI AATATT 1 cut(s) 215
SspMI CTAG 2 cut(s) 498, 1475
StyD4I CCNGG 3 cut(s) 370, 431, 1204
StyI CCWWGG 2 cut(s) 497, 538
TaaI ACNGT 3 cut(s) 238, 385, 908
TaiI ACGT 3 cut(s) 57, 526, 1434
TaqI TCGA 3 cut(s) 60, 569, 895
TasI AATT 5 cut(s) 396, 600, 618, 666, 1486
TatI WGTACW 1 cut(s) 1503
TfiI GAWTC 2 cut(s) 199, 916
Tru1I TTAA 4 cut(s) 222, 603, 1161, 1329
Tru9I TTAA 4 cut(s) 222, 603, 1161, 1329
TscAI CASTG 4 cut(s) 123, 241, 390, 1242
TseFI GTSAC 2 cut(s) 349, 427
TseI GCWGC 2 cut(s) 794, 1214
Tsp45I GTSAC 2 cut(s) 349, 427
TspDTI ATGAA 8 cut(s) 41, 345, 578, 856, 955, 1073, 1247, 1452
TspRI CASTG 4 cut(s) 123, 241, 390, 1242
Tth111I GACNNNGTC 1 cut(s) 1223
Van91I CCANNNNNTGG 1 cut(s) 1450
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 299
VspI ATTAAT 1 cut(s) 1161
XceI RCATGY 2 cut(s) 341, 421
XcmI CCANNNNNNNNNTGG 2 cut(s) 524, 898
XmaJI CCTAGG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 1227
XmnI GAANNNNTTC 1 cut(s) 1312
XspI CTAG 2 cut(s) 498, 1475
ZrmI AGTACT 1 cut(s) 1505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.