AT4G32840

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000762
Physical Location & Seq
Reverse (-)
15844576 .. 15848552
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32840.1

Sequence Viewer

Length: 1389 bp
ATGGCTTCTAATGGCGTGGACGAGCAGATTAAGTTGGTGGAAGGACCTGCTGGTTATGTACTCGAAGATGTTCCTCATTTGAGCGATTACATTCTCGATCTCCCCACTTATCCAAACCCGTTGCAATCAAATGCTGCTTATTCAGTAGTAAGGCAGTATTTTGTTGATGAGGATGATACAGTCCAGGAAAAGATTGTTGTTCACAAGGATTCTCCTAGAGGGACACATTTTAGACGTGCAGGCCCGCGCCAAAAGGTGTACTTCAAGCCATCTGATGTGCGTGCCTGTATTGTCACTTGTGGTGGCCTATGCCCCGGGCTTAATACAGTTATCAGGGAGATCGTGTGTGGTCTTCATTTTATGTATGGTGTTACAGAAGTGATTGGTGTGGATTGCGGGTTTCGCGGGTTCTATTCTAAAAACACTGTTGCACTAACGCCGAAGACTGTGAGTGACATCCACAAGCGCGGGGGAACCATACTAGGAACCTCCCGAGGGGGACATGATACATCAAAGATTGTCGACAACATACAAGATCGTGAAATAAATCAGGTTTACATTATTGGAGGTGATGGAACCCAAAAGGGAGCAAATGCAATATATAAGGAAATTAGGCGCCGTGGACTTAAAGTTGCAGTTGCAGGAATACCAAAAACCATTGACAACGACATCCCAGTCATTGACAAGTCCTTTGGATTTGATACTGCGGTTGAGGAGGCTCAACGTGCTATTAATGCAGCGCATGTTGAAGCGACCAGTGTGGAAAACGGCATTGGAATTGTCAAGCTAATGGGTCGGTACAGTGGGTTCATTGCAATGTATGCTACTCTGGCTAGCCGAGATGTTGACTGCTGTCTGATTCCCGAGTCACCCTTTTACCTTGAAGGCAAAGGAGGGCTCTATGAGTTCATTGCAAAACGTCTCAGAGAAAATGGCCACATGGTTATAGTGATTGCTGAAGGAGCAGGACAGGATCTTGTTGCAGAGAGCATTGAACAACAAGATGCTTCAGGAAACAAGCTCCTTAAAGATGTAGGACTATGGATGAGTCTTAAAATCAAGGAATACTTTGCGAAGCACAATGTAATGGACATTACTCTTAAATACATTGATCCGACATACATGATCCGAGCTATTCCAGCCAATGCATCTGACAACGTCTACTCAACTCTCCTTGCCCAAAGCGCAGTCCATGGTGCAATGGCTGGCTATACCGGCTTTGTCTCTGGTCTTGTTAATGGAAGGCACACCTACATTCCCTTTAACCGAATAACCGAAAGACAGAACAAAGTTGTGATTACAGATAGAATGTGGGCGCGAATGTTGTCATCTACAAACCAGCCCAGCTTCATGAACCCTCCTAAAGGAACCACGGAGTTTACCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0048364 GO:0048731 GO:0048856 GO:0048869 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0090558 GO:0090627 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

462

Amino Acids

50.79

Weight (kDa)

6.61

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 95 - 398 4.8e-63 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 55
AccB1I GGYRCC 1 cut(s) 615
AccI GTMKAC 2 cut(s) 522, 1161
AccII CGCG 4 cut(s) 247, 405, 468, 1318
AciI CCGC 5 cut(s) 245, 396, 405, 468, 707
AclWI GGATC 3 cut(s) 981, 1106, 1120
AcoI YGGCCR 1 cut(s) 934
AcuI CTGAAG 2 cut(s) 978, 993
AcyI GRCGYC 1 cut(s) 616
AfaI GTAC 3 cut(s) 60, 260, 800
AfiI CCNNNNNNNGG 2 cut(s) 495, 1364
AgsI TTSAA 4 cut(s) 265, 749, 884, 995
AjiI CACGTC 1 cut(s) 236
AjnI CCWGG 1 cut(s) 183
AjuI GAANNNNNNNTTGG 2 cut(s) 756, 788
AluBI AGCT 4 cut(s) 787, 1021, 1133, 1347
AluI AGCT 4 cut(s) 787, 1021, 1133, 1347
Alw26I GTCTC 2 cut(s) 926, 1228
AlwI GGATC 3 cut(s) 981, 1106, 1120
Ama87I CYCGRG 3 cut(s) 314, 492, 863
AoxI GGCC 3 cut(s) 241, 304, 934
ApeKI GCWGC 2 cut(s) 134, 737
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AseI ATTAAT 1 cut(s) 732
Asp700I GAANNNNTTC 1 cut(s) 69
AspLEI GCGC 6 cut(s) 249, 468, 618, 742, 1187, 1318
AspS9I GGNCC 2 cut(s) 44, 242
AsuC2I CCSGG 2 cut(s) 315, 316
AsuHPI GGTGA 2 cut(s) 581, 861
AsuNHI GCTAGC 1 cut(s) 833
AvaI CYCGRG 3 cut(s) 314, 492, 863
AvaII GGWCC 1 cut(s) 44
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 900
BbsI GAAGAC 2 cut(s) 344, 449
BbvI GCAGC 2 cut(s) 121, 749
BccI CCATC 2 cut(s) 277, 566
BceAI ACGGC 2 cut(s) 603, 784
BciT130I CCWGG 1 cut(s) 185
BcnI CCSGG 2 cut(s) 315, 316
BcoDI GTCTC 2 cut(s) 926, 1228
BfaI CTAG 3 cut(s) 216, 482, 834
BfoI RGCGCY 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 55
BisI GCNGC 2 cut(s) 135, 738
BlsI GCNGC 2 cut(s) 136, 739
Bme1390I CCNGG 3 cut(s) 185, 315, 316
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 3 cut(s) 314, 492, 863
BmgBI CACGTC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 44, 242
BmiI GGNNCC 5 cut(s) 475, 487, 577, 617, 1369
BmrFI CCNGG 3 cut(s) 185, 315, 316
BmrI ACTGGG 1 cut(s) 668
BmsI GCATC 2 cut(s) 994, 1157
BmtI GCTAGC 1 cut(s) 837
BmuI ACTGGG 1 cut(s) 668
BoxI GACNNNNGTC 1 cut(s) 852
BpiI GAAGAC 2 cut(s) 344, 449
BpuMI CCSGG 2 cut(s) 315, 316
BsaHI GRCGYC 1 cut(s) 616
BsaJI CCNNGG 6 cut(s) 313, 314, 493, 619, 1192, 1371
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 1364
Bse118I RCCGGY 1 cut(s) 1214
Bse1I ACTGG 2 cut(s) 674, 756
Bse3DI GCAATG 4 cut(s) 810, 822, 909, 1206
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 6 cut(s) 313, 314, 493, 619, 1192, 1371
BseGI GGATG 4 cut(s) 178, 456, 669, 1050
BseLI CCNNNNNNNGG 2 cut(s) 495, 1364
BseMI GCAATG 4 cut(s) 810, 822, 909, 1206
BseMII CTCAG 1 cut(s) 937
BseNI ACTGG 2 cut(s) 674, 756
BseRI GAGGAG 1 cut(s) 728
BseXI GCAGC 2 cut(s) 121, 749
BseYI CCCAGC 1 cut(s) 1343
BsgI GTGCAG 1 cut(s) 258
Bsh1236I CGCG 4 cut(s) 247, 405, 468, 1318
BshFI GGCC 3 cut(s) 243, 306, 936
BshNI GGYRCC 1 cut(s) 615
BsiHKCI CYCGRG 3 cut(s) 314, 492, 863
BsiSI CCGG 2 cut(s) 315, 1215
BslFI GGGAC 2 cut(s) 235, 513
BslI CCNNNNNNNGG 2 cut(s) 495, 1364
BsmAI GTCTC 2 cut(s) 926, 1228
BsmBI CGTCTC 1 cut(s) 926
BsmFI GGGAC 2 cut(s) 235, 513
BsnI GGCC 3 cut(s) 243, 306, 936
BsoBI CYCGRG 3 cut(s) 314, 492, 863
Bsp1286I GDGCHC 1 cut(s) 900
Bsp143I GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
Bsp19I CCATGG 1 cut(s) 1192
BspACI CCGC 5 cut(s) 245, 396, 405, 468, 707
BspANI GGCC 3 cut(s) 243, 306, 936
BspCNI CTCAG 1 cut(s) 936
BspFNI CGCG 4 cut(s) 247, 405, 468, 1318
BspHI TCATGA 1 cut(s) 1350
BspLI GGNNCC 5 cut(s) 475, 487, 577, 617, 1369
BspMI ACCTGC 1 cut(s) 55
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 3 cut(s) 981, 1106, 1120
BspT107I GGYRCC 1 cut(s) 615
BsrDI GCAATG 4 cut(s) 810, 822, 909, 1206
BsrFI RCCGGY 1 cut(s) 1214
BsrI ACTGG 2 cut(s) 674, 756
BssAI RCCGGY 1 cut(s) 1214
BssECI CCNNGG 6 cut(s) 313, 314, 493, 619, 1192, 1371
BssMI GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
BssNI GRCGYC 1 cut(s) 616
BssT1I CCWWGG 1 cut(s) 1192
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 5 cut(s) 181, 328, 427, 448, 803
BstACI GRCGYC 1 cut(s) 616
BstAPI GCANNNNNTGC 1 cut(s) 821
BstC8I GCNNGC 5 cut(s) 241, 245, 282, 835, 1207
BstDEI CTNAG 1 cut(s) 923
BstDSI CCRYGG 3 cut(s) 619, 1192, 1371
BstENI CCTNNNNNAGG 1 cut(s) 1362
BstF5I GGATG 4 cut(s) 178, 456, 669, 1050
BstFNI CGCG 4 cut(s) 247, 405, 468, 1318
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 6 cut(s) 249, 468, 618, 742, 1187, 1318
BstKTI GATC 6 cut(s) 100, 342, 538, 976, 1114, 1128
BstMAI GTCTC 2 cut(s) 926, 1228
BstMBI GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
BstMWI GCNNNNNNNGC 9 cut(s) 402, 725, 734, 821, 830, 962, 1139, 1184, 1215
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 746
BstPAI GACNNNNGTC 1 cut(s) 852
BstSCI CCNGG 3 cut(s) 183, 313, 314
BstUI CGCG 4 cut(s) 247, 405, 468, 1318
BstV1I GCAGC 2 cut(s) 121, 749
BstV2I GAAGAC 2 cut(s) 344, 449
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BsuRI GGCC 3 cut(s) 243, 306, 936
BtgI CCRYGG 3 cut(s) 619, 1192, 1371
BtrI CACGTC 1 cut(s) 236
BtsCI GGATG 4 cut(s) 178, 456, 669, 1050
BtsIMutI CAGTG 3 cut(s) 423, 763, 808
BveI ACCTGC 1 cut(s) 55
Cac8I GCNNGC 5 cut(s) 241, 245, 282, 835, 1207
CciI TCATGA 1 cut(s) 1350
CfoI GCGC 6 cut(s) 249, 468, 618, 742, 1187, 1318
Cfr10I RCCGGY 1 cut(s) 1214
Cfr13I GGNCC 2 cut(s) 44, 242
Cfr9I CCCGGG 1 cut(s) 314
Csp6I GTAC 3 cut(s) 59, 259, 799
CviAII CATG 6 cut(s) 503, 743, 940, 1123, 1193, 1351
CviQI GTAC 3 cut(s) 59, 259, 799
DdeI CTNAG 1 cut(s) 923
DinI GGCGCC 1 cut(s) 617
DpnI GATC 6 cut(s) 99, 341, 537, 975, 1113, 1127
DpnII GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
DrdI GACNNNNNNGTC 1 cut(s) 674
DseDI GACNNNNNNGTC 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 934
Eco130I CCWWGG 1 cut(s) 1192
Eco24I GRGCYC 1 cut(s) 900
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 2 cut(s) 978, 993
Eco88I CYCGRG 3 cut(s) 314, 492, 863
EcoNI CCTNNNNNAGG 1 cut(s) 1362
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 1192
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 1 cut(s) 900
EgeI GGCGCC 1 cut(s) 617
EheI GGCGCC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 1192
Esp3I CGTCTC 1 cut(s) 926
FaeI CATG 6 cut(s) 506, 746, 943, 1126, 1196, 1354
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 1052, 1084
FaqI GGGAC 2 cut(s) 235, 513
FatI CATG 6 cut(s) 502, 742, 939, 1122, 1192, 1350
FauI CCCGC 4 cut(s) 252, 389, 398, 461
FblI GTMKAC 2 cut(s) 522, 1161
Fnu4HI GCNGC 2 cut(s) 135, 738
FokI GGATG 4 cut(s) 185, 443, 656, 1057
FriOI GRGCYC 1 cut(s) 900
Fsp4HI GCNGC 2 cut(s) 135, 738
FspBI CTAG 3 cut(s) 216, 482, 834
GlaI GCGC 6 cut(s) 248, 467, 617, 741, 1186, 1317
GluI GCNGC 2 cut(s) 135, 738
GsaI CCCAGC 1 cut(s) 1347
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 3 cut(s) 243, 306, 936
HapII CCGG 2 cut(s) 315, 1215
HhaI GCGC 6 cut(s) 249, 468, 618, 742, 1187, 1318
Hin1I GRCGYC 1 cut(s) 616
Hin1II CATG 6 cut(s) 506, 746, 943, 1126, 1196, 1354
Hin6I GCGC 6 cut(s) 247, 466, 616, 740, 1185, 1316
HinP1I GCGC 6 cut(s) 247, 466, 616, 740, 1185, 1316
HincII GTYRAC 2 cut(s) 523, 847
HindII GTYRAC 2 cut(s) 523, 847
HinfI GANTC 4 cut(s) 209, 859, 866, 1048
HpaII CCGG 2 cut(s) 315, 1215
HphI GGTGA 2 cut(s) 581, 861
Hpy166II GTNNAC 9 cut(s) 19, 202, 259, 523, 556, 623, 847, 1162, 1380
Hpy188I TCNGA 6 cut(s) 274, 858, 926, 1116, 1130, 1153
Hpy188III TCNNGA 6 cut(s) 95, 492, 539, 863, 1011, 1351
Hpy8I GTNNAC 9 cut(s) 19, 202, 259, 523, 556, 623, 847, 1162, 1380
HpyAV CCTTC 4 cut(s) 35, 878, 953, 1236
HpyCH4III ACNGT 5 cut(s) 181, 328, 427, 448, 803
HpyCH4IV ACGT 4 cut(s) 235, 724, 919, 1158
HpyF10VI GCNNNNNNNGC 9 cut(s) 402, 725, 734, 821, 830, 962, 1139, 1184, 1215
HpyF3I CTNAG 1 cut(s) 923
HpySE526I ACGT 4 cut(s) 235, 724, 919, 1158
Hsp92I GRCGYC 1 cut(s) 616
Hsp92II CATG 6 cut(s) 506, 746, 943, 1126, 1196, 1354
HspAI GCGC 6 cut(s) 247, 466, 616, 740, 1185, 1316
KasI GGCGCC 1 cut(s) 615
Kzo9I GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
LmnI GCTCC 3 cut(s) 587, 962, 1026
Lsp1109I GCAGC 2 cut(s) 121, 749
LweI GCATC 2 cut(s) 994, 1157
MaeI CTAG 3 cut(s) 216, 482, 834
MaeII ACGT 4 cut(s) 235, 724, 919, 1158
MaeIII GTNAC 4 cut(s) 292, 370, 452, 867
MalI GATC 6 cut(s) 99, 341, 537, 975, 1113, 1127
MboI GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
MboII GAAGA 3 cut(s) 77, 344, 454
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 1 cut(s) 900
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 2 cut(s) 609, 777
MluNI TGGCCA 1 cut(s) 936
Mly113I GGCGCC 1 cut(s) 616
MlyI GAGTC 2 cut(s) 875, 1057
MmeI TCCRAC 1 cut(s) 1139
Mox20I TGGCCA 1 cut(s) 936
Mph1103I ATGCAT 1 cut(s) 1150
MroXI GAANNNNTTC 1 cut(s) 69
MscI TGGCCA 1 cut(s) 936
MseI TTAA 9 cut(s) 30, 321, 627, 732, 1026, 1053, 1101, 1236, 1263
MslI CAYNNNNRTG 1 cut(s) 815
Msp20I TGGCCA 1 cut(s) 936
MspI CCGG 2 cut(s) 315, 1215
MspR9I CCNGG 3 cut(s) 185, 315, 316
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 4 cut(s) 247, 405, 468, 1318
MwoI GCNNNNNNNGC 9 cut(s) 402, 725, 734, 821, 830, 962, 1139, 1184, 1215
NarI GGCGCC 1 cut(s) 616
NciI CCSGG 2 cut(s) 315, 316
NcoI CCATGG 1 cut(s) 1192
NdeII GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 6 cut(s) 506, 746, 943, 1126, 1196, 1354
NlaIV GGNNCC 5 cut(s) 475, 487, 577, 617, 1369
NmeAIII GCCGAG 1 cut(s) 863
NmuCI GTSAC 3 cut(s) 292, 452, 867
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 1 cut(s) 746
PagI TCATGA 1 cut(s) 1350
PdmI GAANNNNTTC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 209, 859
PflFI GACNNNGTC 1 cut(s) 1157
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 2 cut(s) 136, 739
PleI GAGTC 2 cut(s) 874, 1056
PluTI GGCGCC 1 cut(s) 619
PpsI GAGTC 2 cut(s) 874, 1056
PpuMI RGGWCCY 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 852
PshBI ATTAAT 1 cut(s) 732
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 1343
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 5 cut(s) 475, 487, 577, 617, 1369
PspPI GGNCC 2 cut(s) 44, 242
PspPPI RGGWCCY 1 cut(s) 44
PsrI GAACNNNNNNTAC 2 cut(s) 791, 823
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 1157
RsaI GTAC 3 cut(s) 60, 260, 800
RsaNI GTAC 3 cut(s) 59, 259, 799
RseI CAYNNNNRTG 1 cut(s) 815
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 9 cut(s) 30, 321, 627, 732, 1026, 1053, 1101, 1236, 1263
SatI GCNGC 2 cut(s) 135, 738
Sau3AI GATC 6 cut(s) 97, 339, 535, 973, 1111, 1125
Sau96I GGNCC 2 cut(s) 44, 242
SchI GAGTC 2 cut(s) 875, 1057
ScrFI CCNGG 3 cut(s) 185, 315, 316
SduI GDGCHC 1 cut(s) 900
SfaNI GCATC 2 cut(s) 994, 1157
SfoI GGCGCC 1 cut(s) 617
SinI GGWCC 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 815
Sse9I AATT 2 cut(s) 609, 777
SsiI CCGC 5 cut(s) 245, 396, 405, 468, 707
SspDI GGCGCC 1 cut(s) 615
SspMI CTAG 3 cut(s) 216, 482, 834
StyD4I CCNGG 3 cut(s) 183, 313, 314
StyI CCWWGG 1 cut(s) 1192
TaaI ACNGT 5 cut(s) 181, 328, 427, 448, 803
TaiI ACGT 4 cut(s) 238, 727, 922, 1161
TaqI TCGA 3 cut(s) 63, 96, 522
TasI AATT 2 cut(s) 609, 777
TatI WGTACW 2 cut(s) 58, 258
TfiI GAWTC 2 cut(s) 209, 859
Tru1I TTAA 9 cut(s) 30, 321, 627, 732, 1026, 1053, 1101, 1236, 1263
Tru9I TTAA 9 cut(s) 30, 321, 627, 732, 1026, 1053, 1101, 1236, 1263
TscAI CASTG 3 cut(s) 430, 763, 808
TseFI GTSAC 3 cut(s) 292, 452, 867
TseI GCWGC 2 cut(s) 134, 737
Tsp45I GTSAC 3 cut(s) 292, 452, 867
TspDTI ATGAA 5 cut(s) 344, 799, 898, 1339, 1367
TspGWI ACGGA 1 cut(s) 1388
TspMI CCCGGG 1 cut(s) 314
TspRI CASTG 3 cut(s) 430, 763, 808
Tth111I GACNNNGTC 1 cut(s) 1157
VpaK11BI GGWCC 1 cut(s) 44
VspI ATTAAT 1 cut(s) 732
XagI CCTNNNNNAGG 1 cut(s) 1362
XceI RCATGY 1 cut(s) 746
XmaI CCCGGG 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 522, 1161
XmnI GAANNNNTTC 1 cut(s) 69
XspI CTAG 3 cut(s) 216, 482, 834
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.