Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 95 - 400 | 5.1e-63 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 1167 |
| AciI | CCGC | 3 cut(s) | 5, 266, 704 |
| AclWI | GGATC | 5 cut(s) | 678, 978, 1070, 1077, 1112 |
| AcyI | GRCGYC | 1 cut(s) | 613 |
| AfaI | GTAC | 2 cut(s) | 472, 1445 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1244, 1294, 1390 |
| AflIII | ACRYGT | 2 cut(s) | 293, 357 |
| AgeI | ACCGGT | 1 cut(s) | 670 |
| AgsI | TTSAA | 5 cut(s) | 28, 881, 911, 1338, 1360 |
| AjiI | CACGTC | 1 cut(s) | 67 |
| AjnI | CCWGG | 2 cut(s) | 310, 546 |
| AluBI | AGCT | 9 cut(s) | 737, 749, 784, 812, 987, 1027, 1093, 1157, 1353 |
| AluI | AGCT | 9 cut(s) | 737, 749, 784, 812, 987, 1027, 1093, 1157, 1353 |
| Alw21I | GWGCWC | 1 cut(s) | 60 |
| Alw26I | GTCTC | 1 cut(s) | 1416 |
| AlwI | GGATC | 5 cut(s) | 678, 978, 1070, 1077, 1112 |
| AoxI | GGCC | 4 cut(s) | 496, 619, 714, 1235 |
| ApeKI | GCWGC | 2 cut(s) | 734, 1154 |
| AseI | ATTAAT | 1 cut(s) | 729 |
| AsiGI | ACCGGT | 1 cut(s) | 670 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1252 |
| AspA2I | CCTAGG | 1 cut(s) | 437 |
| AspLEI | GCGC | 1 cut(s) | 1287 |
| AspS9I | GGNCC | 3 cut(s) | 239, 715, 1235 |
| AsuHPI | GGTGA | 2 cut(s) | 26, 337 |
| AvaII | GGWCC | 1 cut(s) | 239 |
| AvrII | CCTAGG | 1 cut(s) | 437 |
| BaeGI | GKGCMC | 2 cut(s) | 311, 1324 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 690, 723 |
| BanII | GRGCYC | 1 cut(s) | 318 |
| BbsI | GAAGAC | 3 cut(s) | 69, 174, 1255 |
| Bbv12I | GWGCWC | 1 cut(s) | 60 |
| BbvCI | CCTCAGC | 1 cut(s) | 954 |
| BbvI | GCAGC | 2 cut(s) | 746, 1166 |
| BccI | CCATC | 2 cut(s) | 383, 563 |
| BceAI | ACGGC | 3 cut(s) | 600, 1178, 1340 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 161, 195 |
| BciT130I | CCWGG | 2 cut(s) | 312, 548 |
| BclI | TGATCA | 2 cut(s) | 327, 1131 |
| BcoDI | GTCTC | 1 cut(s) | 1416 |
| BfaI | CTAG | 3 cut(s) | 438, 1415, 1447 |
| BfmI | CTRYAG | 2 cut(s) | 141, 796 |
| BfoI | RGCGCY | 1 cut(s) | 1288 |
| BisI | GCNGC | 2 cut(s) | 735, 1155 |
| BlnI | CCTAGG | 1 cut(s) | 437 |
| BlsI | GCNGC | 2 cut(s) | 736, 1156 |
| BmcAI | AGTACT | 1 cut(s) | 1445 |
| Bme1390I | CCNGG | 2 cut(s) | 312, 548 |
| Bme18I | GGWCC | 1 cut(s) | 239 |
| BmgBI | CACGTC | 1 cut(s) | 67 |
| BmgT120I | GGNCC | 3 cut(s) | 239, 715, 1235 |
| BmiI | GGNNCC | 1 cut(s) | 1328 |
| BmrFI | CCNGG | 2 cut(s) | 312, 548 |
| BmsI | GCATC | 2 cut(s) | 596, 1000 |
| BpiI | GAAGAC | 3 cut(s) | 69, 174, 1255 |
| Bpu10I | CCTNAGC | 1 cut(s) | 954 |
| BsaAI | YACGTR | 1 cut(s) | 55 |
| BsaHI | GRCGYC | 1 cut(s) | 613 |
| BsaJI | CCNNGG | 5 cut(s) | 310, 311, 437, 478, 616 |
| BsaWI | WCCGGW | 2 cut(s) | 11, 670 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 576, 606 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1244, 1294, 1390 |
| Bse118I | RCCGGY | 2 cut(s) | 47, 670 |
| Bse1I | ACTGG | 1 cut(s) | 831 |
| Bse3DI | GCAATG | 1 cut(s) | 1212 |
| BseBI | CCWGG | 2 cut(s) | 312, 548 |
| BseDI | CCNNGG | 5 cut(s) | 310, 311, 437, 478, 616 |
| BseGI | GGATG | 2 cut(s) | 274, 1320 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1244, 1294, 1390 |
| BseMI | GCAATG | 1 cut(s) | 1212 |
| BseMII | CTCAG | 3 cut(s) | 945, 1070, 1272 |
| BseNI | ACTGG | 1 cut(s) | 831 |
| BseRI | GAGGAG | 2 cut(s) | 597, 725 |
| BseSI | GKGCMC | 2 cut(s) | 311, 1324 |
| BseXI | GCAGC | 2 cut(s) | 746, 1166 |
| BseYI | CCCAGC | 1 cut(s) | 717 |
| BsgI | GTGCAG | 1 cut(s) | 1156 |
| BshFI | GGCC | 4 cut(s) | 498, 621, 716, 1237 |
| BshTI | ACCGGT | 1 cut(s) | 670 |
| BsiHKAI | GWGCWC | 1 cut(s) | 60 |
| BsiSI | CCGG | 3 cut(s) | 12, 48, 671 |
| BslFI | GGGAC | 4 cut(s) | 232, 496, 804, 850 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1244, 1294, 1390 |
| BsmAI | GTCTC | 1 cut(s) | 1416 |
| BsmFI | GGGAC | 4 cut(s) | 232, 496, 804, 850 |
| BsmI | GAATGC | 1 cut(s) | 525 |
| BsnI | GGCC | 4 cut(s) | 498, 621, 716, 1237 |
| Bsp1286I | GDGCHC | 4 cut(s) | 60, 311, 318, 1324 |
| BspACI | CCGC | 3 cut(s) | 5, 266, 704 |
| BspANI | GGCC | 4 cut(s) | 498, 621, 716, 1237 |
| BspCNI | CTCAG | 3 cut(s) | 946, 1069, 1273 |
| BspHI | TCATGA | 1 cut(s) | 529 |
| BspLI | GGNNCC | 1 cut(s) | 1328 |
| BspPI | GGATC | 5 cut(s) | 678, 978, 1070, 1077, 1112 |
| BspQI | GCTCTTC | 1 cut(s) | 1389 |
| BsrDI | GCAATG | 1 cut(s) | 1212 |
| BsrFI | RCCGGY | 2 cut(s) | 47, 670 |
| BsrI | ACTGG | 1 cut(s) | 831 |
| BssAI | RCCGGY | 2 cut(s) | 47, 670 |
| BssECI | CCNNGG | 5 cut(s) | 310, 311, 437, 478, 616 |
| BssNI | GRCGYC | 1 cut(s) | 613 |
| BssT1I | CCWWGG | 2 cut(s) | 437, 478 |
| Bst2UI | CCWGG | 2 cut(s) | 312, 548 |
| Bst4CI | ACNGT | 5 cut(s) | 178, 325, 424, 800, 848 |
| Bst6I | CTCTTC | 2 cut(s) | 975, 1389 |
| BstACI | GRCGYC | 1 cut(s) | 613 |
| BstBAI | YACGTR | 1 cut(s) | 55 |
| BstC8I | GCNNGC | 5 cut(s) | 234, 279, 723, 989, 1182 |
| BstDEI | CTNAG | 3 cut(s) | 954, 1056, 1281 |
| BstDSI | CCRYGG | 1 cut(s) | 616 |
| BstEII | GGTNACC | 1 cut(s) | 14 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| BstF5I | GGATG | 2 cut(s) | 274, 1320 |
| BstH2I | RGCGCY | 1 cut(s) | 1288 |
| BstHHI | GCGC | 1 cut(s) | 1287 |
| BstMAI | GTCTC | 1 cut(s) | 1416 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1190 |
| BstNI | CCWGG | 2 cut(s) | 312, 548 |
| BstNSI | RCATGY | 3 cut(s) | 281, 297, 361 |
| BstPI | GGTNACC | 1 cut(s) | 14 |
| BstSCI | CCNGG | 2 cut(s) | 310, 546 |
| BstSFI | CTRYAG | 2 cut(s) | 141, 796 |
| BstSLI | GKGCMC | 2 cut(s) | 311, 1324 |
| BstV1I | GCAGC | 2 cut(s) | 746, 1166 |
| BstV2I | GAAGAC | 3 cut(s) | 69, 174, 1255 |
| BstX2I | RGATCY | 3 cut(s) | 683, 970, 1117 |
| BstYI | RGATCY | 3 cut(s) | 683, 970, 1117 |
| BsuRI | GGCC | 4 cut(s) | 498, 621, 716, 1237 |
| BtgI | CCRYGG | 1 cut(s) | 616 |
| BtgZI | GCGATG | 1 cut(s) | 582 |
| BtrI | CACGTC | 1 cut(s) | 67 |
| BtsCI | GGATG | 2 cut(s) | 274, 1320 |
| BtsI | GCAGTG | 2 cut(s) | 116, 1175 |
| BtsIMutI | CAGTG | 4 cut(s) | 116, 805, 1175, 1278 |
| Cac8I | GCNNGC | 5 cut(s) | 234, 279, 723, 989, 1182 |
| CciI | TCATGA | 1 cut(s) | 529 |
| CfoI | GCGC | 1 cut(s) | 1287 |
| Cfr10I | RCCGGY | 2 cut(s) | 47, 670 |
| Cfr13I | GGNCC | 3 cut(s) | 239, 715, 1235 |
| CseI | GACGC | 2 cut(s) | 621, 801 |
| CsiI | ACCWGGT | 1 cut(s) | 546 |
| Csp6I | GTAC | 2 cut(s) | 471, 1444 |
| CspAI | ACCGGT | 1 cut(s) | 670 |
| CviAII | CATG | 9 cut(s) | 278, 294, 358, 500, 530, 740, 997, 1129, 1199 |
| CviQI | GTAC | 2 cut(s) | 471, 1444 |
| DdeI | CTNAG | 3 cut(s) | 954, 1056, 1281 |
| Eam1104I | CTCTTC | 2 cut(s) | 975, 1389 |
| EarI | CTCTTC | 2 cut(s) | 975, 1389 |
| EciI | GGCGGA | 1 cut(s) | 281 |
| Eco130I | CCWWGG | 2 cut(s) | 437, 478 |
| Eco24I | GRGCYC | 1 cut(s) | 318 |
| Eco32I | GATATC | 1 cut(s) | 1053 |
| Eco47I | GGWCC | 1 cut(s) | 239 |
| Eco91I | GGTNACC | 1 cut(s) | 14 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| EcoO65I | GGTNACC | 1 cut(s) | 14 |
| EcoRII | CCWGG | 2 cut(s) | 310, 546 |
| EcoRV | GATATC | 1 cut(s) | 1053 |
| EcoT14I | CCWWGG | 2 cut(s) | 437, 478 |
| EcoT38I | GRGCYC | 1 cut(s) | 318 |
| ErhI | CCWWGG | 2 cut(s) | 437, 478 |
| FaeI | CATG | 9 cut(s) | 281, 297, 361, 503, 533, 743, 1000, 1132, 1202 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1058, 1090, 1240, 1272 |
| FaqI | GGGAC | 4 cut(s) | 232, 496, 804, 850 |
| FatI | CATG | 9 cut(s) | 277, 293, 357, 499, 529, 739, 996, 1128, 1198 |
| FauNDI | CATATG | 1 cut(s) | 936 |
| FbaI | TGATCA | 2 cut(s) | 327, 1131 |
| FblI | GTMKAC | 1 cut(s) | 1167 |
| Fnu4HI | GCNGC | 2 cut(s) | 735, 1155 |
| FokI | GGATG | 2 cut(s) | 281, 1327 |
| FriOI | GRGCYC | 1 cut(s) | 318 |
| Fsp4HI | GCNGC | 2 cut(s) | 735, 1155 |
| FspBI | CTAG | 3 cut(s) | 438, 1415, 1447 |
| GlaI | GCGC | 1 cut(s) | 1286 |
| GluI | GCNGC | 2 cut(s) | 735, 1155 |
| GsaI | CCCAGC | 1 cut(s) | 721 |
| HaeII | RGCGCY | 1 cut(s) | 1288 |
| HaeIII | GGCC | 4 cut(s) | 498, 621, 716, 1237 |
| HapII | CCGG | 3 cut(s) | 12, 48, 671 |
| HgaI | GACGC | 2 cut(s) | 621, 801 |
| HhaI | GCGC | 1 cut(s) | 1287 |
| Hin1I | GRCGYC | 1 cut(s) | 613 |
| Hin1II | CATG | 9 cut(s) | 281, 297, 361, 503, 533, 743, 1000, 1132, 1202 |
| Hin6I | GCGC | 1 cut(s) | 1285 |
| HinP1I | GCGC | 1 cut(s) | 1285 |
| HincII | GTYRAC | 3 cut(s) | 163, 370, 520 |
| HindII | GTYRAC | 3 cut(s) | 163, 370, 520 |
| HinfI | GANTC | 3 cut(s) | 856, 863, 918 |
| HpaI | GTTAAC | 1 cut(s) | 163 |
| HpaII | CCGG | 3 cut(s) | 12, 48, 671 |
| HphI | GGTGA | 2 cut(s) | 26, 337 |
| Hpy188I | TCNGA | 5 cut(s) | 94, 383, 1059, 1107, 1146 |
| Hpy188III | TCNNGA | 8 cut(s) | 414, 530, 836, 860, 878, 977, 1017, 1357 |
| HpyAV | CCTTC | 4 cut(s) | 236, 875, 880, 1412 |
| HpyCH4III | ACNGT | 5 cut(s) | 178, 325, 424, 800, 848 |
| HpyCH4IV | ACGT | 4 cut(s) | 54, 66, 463, 1371 |
| HpyCH4V | TGCA | 7 cut(s) | 121, 277, 734, 991, 1079, 1154, 1173 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1190 |
| HpyF3I | CTNAG | 3 cut(s) | 954, 1056, 1281 |
| HpySE526I | ACGT | 4 cut(s) | 54, 66, 463, 1371 |
| Hsp92I | GRCGYC | 1 cut(s) | 613 |
| Hsp92II | CATG | 9 cut(s) | 281, 297, 361, 503, 533, 743, 1000, 1132, 1202 |
| HspAI | GCGC | 1 cut(s) | 1285 |
| Ksp22I | TGATCA | 2 cut(s) | 327, 1131 |
| KspAI | GTTAAC | 1 cut(s) | 163 |
| LguI | GCTCTTC | 1 cut(s) | 1389 |
| LmnI | GCTCC | 4 cut(s) | 584, 959, 1202, 1332 |
| Lsp1109I | GCAGC | 2 cut(s) | 746, 1166 |
| LweI | GCATC | 2 cut(s) | 596, 1000 |
| MabI | ACCWGGT | 1 cut(s) | 546 |
| MaeI | CTAG | 3 cut(s) | 438, 1415, 1447 |
| MaeII | ACGT | 4 cut(s) | 54, 66, 463, 1371 |
| MaeIII | GTNAC | 4 cut(s) | 14, 50, 289, 1402 |
| MfeI | CAATTG | 1 cut(s) | 1426 |
| MflI | RGATCY | 3 cut(s) | 683, 970, 1117 |
| MhlI | GDGCHC | 4 cut(s) | 60, 311, 318, 1324 |
| MluCI | AATT | 3 cut(s) | 336, 540, 1426 |
| MlyI | GAGTC | 2 cut(s) | 872, 912 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1252 |
| MseI | TTAA | 5 cut(s) | 162, 543, 729, 1032, 1269 |
| MslI | CAYNNNNRTG | 4 cut(s) | 360, 462, 534, 812 |
| MspA1I | CMGCKG | 1 cut(s) | 1157 |
| MspI | CCGG | 3 cut(s) | 12, 48, 671 |
| MspR9I | CCNGG | 2 cut(s) | 312, 548 |
| MunI | CAATTG | 1 cut(s) | 1426 |
| Mva1269I | GAATGC | 1 cut(s) | 525 |
| MvaI | CCWGG | 2 cut(s) | 312, 548 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 722, 731, 818, 827, 959, 1190 |
| NdeI | CATATG | 1 cut(s) | 936 |
| NlaIII | CATG | 9 cut(s) | 281, 297, 361, 503, 533, 743, 1000, 1132, 1202 |
| NlaIV | GGNNCC | 1 cut(s) | 1328 |
| NmeAIII | GCCGAG | 1 cut(s) | 1374 |
| NmuCI | GTSAC | 1 cut(s) | 14 |
| NspI | RCATGY | 3 cut(s) | 281, 297, 361 |
| PaeI | GCATGC | 1 cut(s) | 281 |
| PagI | TCATGA | 1 cut(s) | 529 |
| PasI | CCCWGGG | 1 cut(s) | 311 |
| PciI | ACATGT | 2 cut(s) | 293, 357 |
| PciSI | GCTCTTC | 1 cut(s) | 1389 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 39 |
| PctI | GAATGC | 1 cut(s) | 525 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1252 |
| PfeI | GAWTC | 1 cut(s) | 856 |
| PflFI | GACNNNGTC | 1 cut(s) | 1163 |
| PinAI | ACCGGT | 1 cut(s) | 670 |
| PkrI | GCNGC | 2 cut(s) | 736, 1156 |
| PleI | GAGTC | 2 cut(s) | 871, 912 |
| PpsI | GAGTC | 2 cut(s) | 871, 912 |
| Ppu21I | YACGTR | 1 cut(s) | 55 |
| PscI | ACATGT | 2 cut(s) | 293, 357 |
| PshBI | ATTAAT | 1 cut(s) | 729 |
| Psp6I | CCWGG | 2 cut(s) | 310, 546 |
| PspEI | GGTNACC | 1 cut(s) | 14 |
| PspFI | CCCAGC | 1 cut(s) | 717 |
| PspGI | CCWGG | 2 cut(s) | 310, 546 |
| PspN4I | GGNNCC | 1 cut(s) | 1328 |
| PspPI | GGNCC | 3 cut(s) | 239, 715, 1235 |
| PsuI | RGATCY | 3 cut(s) | 683, 970, 1117 |
| PsyI | GACNNNGTC | 1 cut(s) | 1163 |
| PvuII | CAGCTG | 1 cut(s) | 1157 |
| RsaI | GTAC | 2 cut(s) | 472, 1445 |
| RsaNI | GTAC | 2 cut(s) | 471, 1444 |
| RseI | CAYNNNNRTG | 4 cut(s) | 360, 462, 534, 812 |
| SapI | GCTCTTC | 1 cut(s) | 1389 |
| SaqAI | TTAA | 5 cut(s) | 162, 543, 729, 1032, 1269 |
| SatI | GCNGC | 2 cut(s) | 735, 1155 |
| Sau96I | GGNCC | 3 cut(s) | 239, 715, 1235 |
| ScaI | AGTACT | 1 cut(s) | 1445 |
| SchI | GAGTC | 2 cut(s) | 872, 912 |
| ScrFI | CCNGG | 2 cut(s) | 312, 548 |
| SduI | GDGCHC | 4 cut(s) | 60, 311, 318, 1324 |
| SexAI | ACCWGGT | 1 cut(s) | 546 |
| SfaNI | GCATC | 2 cut(s) | 596, 1000 |
| SfcI | CTRYAG | 2 cut(s) | 141, 796 |
| SinI | GGWCC | 1 cut(s) | 239 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 360, 462, 534, 812 |
| SphI | GCATGC | 1 cut(s) | 281 |
| Sse9I | AATT | 3 cut(s) | 336, 540, 1426 |
| SsiI | CCGC | 3 cut(s) | 5, 266, 704 |
| SspMI | CTAG | 3 cut(s) | 438, 1415, 1447 |
| StyD4I | CCNGG | 2 cut(s) | 310, 546 |
| StyI | CCWWGG | 2 cut(s) | 437, 478 |
| TaaI | ACNGT | 5 cut(s) | 178, 325, 424, 800, 848 |
| TaiI | ACGT | 4 cut(s) | 57, 69, 466, 1374 |
| TaqI | TCGA | 3 cut(s) | 60, 509, 835 |
| TasI | AATT | 3 cut(s) | 336, 540, 1426 |
| TatI | WGTACW | 1 cut(s) | 1443 |
| TfiI | GAWTC | 1 cut(s) | 856 |
| Tru1I | TTAA | 5 cut(s) | 162, 543, 729, 1032, 1269 |
| Tru9I | TTAA | 5 cut(s) | 162, 543, 729, 1032, 1269 |
| TscAI | CASTG | 4 cut(s) | 123, 805, 1182, 1285 |
| TseFI | GTSAC | 1 cut(s) | 14 |
| TseI | GCWGC | 2 cut(s) | 734, 1154 |
| Tsp45I | GTSAC | 1 cut(s) | 14 |
| TspDTI | ATGAA | 8 cut(s) | 285, 446, 518, 796, 895, 1013, 1187, 1392 |
| TspRI | CASTG | 4 cut(s) | 123, 805, 1182, 1285 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1163 |
| VpaK11BI | GGWCC | 1 cut(s) | 239 |
| VspI | ATTAAT | 1 cut(s) | 729 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1388 |
| XceI | RCATGY | 3 cut(s) | 281, 297, 361 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 838 |
| XmaJI | CCTAGG | 1 cut(s) | 437 |
| XmiI | GTMKAC | 1 cut(s) | 1167 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1252 |
| XspI | CTAG | 3 cut(s) | 438, 1415, 1447 |
| ZrmI | AGTACT | 1 cut(s) | 1445 |