Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 136 - 441 | 1.3e-61 | Phosphofructokinase |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1001 |
| AatII | GACGTC | 1 cut(s) | 192 |
| Acc36I | ACCTGC | 1 cut(s) | 1001 |
| AccI | GTMKAC | 1 cut(s) | 1290 |
| AccII | CGCG | 1 cut(s) | 367 |
| AciI | CCGC | 4 cut(s) | 70, 73, 135, 365 |
| AclWI | GGATC | 2 cut(s) | 1235, 1249 |
| AcoI | YGGCCR | 4 cut(s) | 3, 619, 1054, 1533 |
| AcsI | RAATTY | 3 cut(s) | 129, 498, 765 |
| AcuI | CTGAAG | 3 cut(s) | 61, 1098, 1508 |
| AcyI | GRCGYC | 2 cut(s) | 56, 189 |
| AdeI | CACNNNGTG | 1 cut(s) | 617 |
| AfaI | GTAC | 4 cut(s) | 476, 485, 656, 697 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 964 |
| AflIII | ACRYGT | 2 cut(s) | 416, 480 |
| AgsI | TTSAA | 5 cut(s) | 386, 869, 1025, 1034, 1157 |
| AjiI | CACGTC | 2 cut(s) | 356, 964 |
| AjnI | CCWGG | 2 cut(s) | 101, 433 |
| AluBI | AGCT | 7 cut(s) | 170, 710, 860, 1076, 1096, 1103, 1150 |
| AluI | AGCT | 7 cut(s) | 170, 710, 860, 1076, 1096, 1103, 1150 |
| Alw26I | GTCTC | 2 cut(s) | 954, 1402 |
| AlwI | GGATC | 2 cut(s) | 1235, 1249 |
| Ama87I | CYCGRG | 1 cut(s) | 683 |
| AoxI | GGCC | 6 cut(s) | 3, 619, 742, 1054, 1165, 1533 |
| ApeKI | GCWGC | 2 cut(s) | 50, 857 |
| ApoI | RAATTY | 3 cut(s) | 129, 498, 765 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 794, 826 |
| AseI | ATTAAT | 2 cut(s) | 666, 852 |
| AspS9I | GGNCC | 3 cut(s) | 362, 1192, 1483 |
| AsuHPI | GGTGA | 5 cut(s) | 25, 151, 981, 1342, 1411 |
| AvaI | CYCGRG | 1 cut(s) | 683 |
| AvaII | GGWCC | 3 cut(s) | 362, 1192, 1483 |
| BaeGI | GKGCMC | 1 cut(s) | 1447 |
| BalI | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| BauI | CACGAG | 1 cut(s) | 612 |
| BbsI | GAAGAC | 1 cut(s) | 192 |
| BbvI | GCAGC | 2 cut(s) | 37, 869 |
| BccI | CCATC | 3 cut(s) | 686, 1134, 1401 |
| BceAI | ACGGC | 3 cut(s) | 812, 1069, 1463 |
| BciT130I | CCWGG | 2 cut(s) | 103, 435 |
| BciVI | GTATCC | 1 cut(s) | 512 |
| BcoDI | GTCTC | 2 cut(s) | 954, 1402 |
| BfaI | CTAG | 1 cut(s) | 1100 |
| BfmI | CTRYAG | 1 cut(s) | 919 |
| BfuAI | ACCTGC | 1 cut(s) | 1001 |
| BfuI | GTATCC | 1 cut(s) | 512 |
| BisI | GCNGC | 3 cut(s) | 51, 73, 858 |
| BlsI | GCNGC | 3 cut(s) | 52, 74, 859 |
| Bme1390I | CCNGG | 2 cut(s) | 103, 435 |
| Bme18I | GGWCC | 3 cut(s) | 362, 1192, 1483 |
| BmeT110I | CYCGRG | 1 cut(s) | 683 |
| BmgBI | CACGTC | 2 cut(s) | 356, 964 |
| BmgT120I | GGNCC | 3 cut(s) | 362, 1192, 1483 |
| BmrFI | CCNGG | 2 cut(s) | 103, 435 |
| BmsI | GCATC | 1 cut(s) | 1123 |
| BpiI | GAAGAC | 1 cut(s) | 192 |
| BsaAI | YACGTR | 1 cut(s) | 228 |
| BsaHI | GRCGYC | 2 cut(s) | 56, 189 |
| BsaJI | CCNNGG | 3 cut(s) | 101, 114, 601 |
| BsaWI | WCCGGW | 2 cut(s) | 388, 793 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 82, 112, 699, 729 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 964 |
| Bse118I | RCCGGY | 1 cut(s) | 161 |
| Bse1I | ACTGG | 1 cut(s) | 1536 |
| Bse3DI | GCAATG | 1 cut(s) | 1335 |
| BseBI | CCWGG | 2 cut(s) | 103, 435 |
| BseDI | CCNNGG | 3 cut(s) | 101, 114, 601 |
| BseGI | GGATG | 4 cut(s) | 210, 397, 576, 1138 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 964 |
| BseMI | GCAATG | 1 cut(s) | 1335 |
| BseNI | ACTGG | 1 cut(s) | 1536 |
| BseRI | GAGGAG | 2 cut(s) | 848, 1526 |
| BseSI | GKGCMC | 1 cut(s) | 1447 |
| BseXI | GCAGC | 2 cut(s) | 37, 869 |
| BseYI | CCCAGC | 1 cut(s) | 144 |
| BsgI | GTGCAG | 2 cut(s) | 1104, 1279 |
| Bsh1236I | CGCG | 1 cut(s) | 367 |
| BshFI | GGCC | 6 cut(s) | 5, 621, 744, 1056, 1167, 1535 |
| BsiHKCI | CYCGRG | 1 cut(s) | 683 |
| BsiSI | CCGG | 3 cut(s) | 162, 389, 794 |
| BslFI | GGGAC | 2 cut(s) | 176, 619 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 964 |
| BsmAI | GTCTC | 2 cut(s) | 954, 1402 |
| BsmBI | CGTCTC | 1 cut(s) | 954 |
| BsmFI | GGGAC | 2 cut(s) | 176, 619 |
| BsmI | GAATGC | 2 cut(s) | 648, 734 |
| BsnI | GGCC | 6 cut(s) | 5, 621, 744, 1056, 1167, 1535 |
| BsoBI | CYCGRG | 1 cut(s) | 683 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1447 |
| Bsp143I | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| BspACI | CCGC | 4 cut(s) | 70, 73, 135, 365 |
| BspANI | GGCC | 6 cut(s) | 5, 621, 744, 1056, 1167, 1535 |
| BspFNI | CGCG | 1 cut(s) | 367 |
| BspMI | ACCTGC | 1 cut(s) | 1001 |
| BspPI | GGATC | 2 cut(s) | 1235, 1249 |
| BsrDI | GCAATG | 1 cut(s) | 1335 |
| BsrFI | RCCGGY | 1 cut(s) | 161 |
| BsrI | ACTGG | 1 cut(s) | 1536 |
| BssAI | RCCGGY | 1 cut(s) | 161 |
| BssECI | CCNNGG | 3 cut(s) | 101, 114, 601 |
| BssMI | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| BssNAI | GTATAC | 1 cut(s) | 1291 |
| BssNI | GRCGYC | 2 cut(s) | 56, 189 |
| BssSI | CACGAG | 1 cut(s) | 612 |
| BssT1I | CCWWGG | 2 cut(s) | 114, 601 |
| Bst1107I | GTATAC | 1 cut(s) | 1291 |
| Bst2BI | CACGAG | 1 cut(s) | 612 |
| Bst2UI | CCWGG | 2 cut(s) | 103, 435 |
| Bst4CI | ACNGT | 4 cut(s) | 448, 488, 923, 1354 |
| BstACI | GRCGYC | 2 cut(s) | 56, 189 |
| BstBAI | YACGTR | 1 cut(s) | 228 |
| BstC8I | GCNNGC | 5 cut(s) | 172, 955, 1112, 1116, 1377 |
| BstF5I | GGATG | 4 cut(s) | 210, 397, 576, 1138 |
| BstFNI | CGCG | 1 cut(s) | 367 |
| BstKTI | GATC | 5 cut(s) | 64, 156, 220, 1243, 1257 |
| BstMAI | GTCTC | 2 cut(s) | 954, 1402 |
| BstMBI | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 11, 78, 171, 845, 854, 941, 950, 1082 |
| BstNI | CCWGG | 2 cut(s) | 103, 435 |
| BstNSI | RCATGY | 5 cut(s) | 408, 420, 484, 1114, 1379 |
| BstSCI | CCNGG | 2 cut(s) | 101, 433 |
| BstSFI | CTRYAG | 1 cut(s) | 919 |
| BstSLI | GKGCMC | 1 cut(s) | 1447 |
| BstSNI | TACGTA | 1 cut(s) | 228 |
| BstUI | CGCG | 1 cut(s) | 367 |
| BstV1I | GCAGC | 2 cut(s) | 37, 869 |
| BstV2I | GAAGAC | 1 cut(s) | 192 |
| BstX2I | RGATCY | 1 cut(s) | 1240 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 587 |
| BstYI | RGATCY | 1 cut(s) | 1240 |
| BstZ17I | GTATAC | 1 cut(s) | 1291 |
| BsuI | GTATCC | 1 cut(s) | 512 |
| BsuRI | GGCC | 6 cut(s) | 5, 621, 744, 1056, 1167, 1535 |
| BtrI | CACGTC | 2 cut(s) | 356, 964 |
| BtsCI | GGATG | 4 cut(s) | 210, 397, 576, 1138 |
| BtsI | GCAGTG | 2 cut(s) | 762, 822 |
| BtsIMutI | CAGTG | 3 cut(s) | 762, 822, 928 |
| BveI | ACCTGC | 1 cut(s) | 1001 |
| Cac8I | GCNNGC | 5 cut(s) | 172, 955, 1112, 1116, 1377 |
| Cfr10I | RCCGGY | 1 cut(s) | 161 |
| Cfr13I | GGNCC | 3 cut(s) | 362, 1192, 1483 |
| CseI | GACGC | 3 cut(s) | 64, 356, 1266 |
| Csp6I | GTAC | 4 cut(s) | 475, 484, 655, 696 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 621, 656 |
| CviQI | GTAC | 4 cut(s) | 475, 484, 655, 696 |
| DpnI | GATC | 5 cut(s) | 63, 155, 219, 1242, 1256 |
| DpnII | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| DraIII | CACNNNGTG | 1 cut(s) | 617 |
| EaeI | YGGCCR | 4 cut(s) | 3, 619, 1054, 1533 |
| EciI | GGCGGA | 1 cut(s) | 124 |
| Eco105I | TACGTA | 1 cut(s) | 228 |
| Eco130I | CCWWGG | 2 cut(s) | 114, 601 |
| Eco47I | GGWCC | 3 cut(s) | 362, 1192, 1483 |
| Eco57I | CTGAAG | 3 cut(s) | 61, 1098, 1508 |
| Eco88I | CYCGRG | 1 cut(s) | 683 |
| EcoRI | GAATTC | 2 cut(s) | 498, 765 |
| EcoRII | CCWGG | 2 cut(s) | 101, 433 |
| EcoT14I | CCWWGG | 2 cut(s) | 114, 601 |
| EcoT22I | ATGCAT | 1 cut(s) | 406 |
| ErhI | CCWWGG | 2 cut(s) | 114, 601 |
| Esp3I | CGTCTC | 1 cut(s) | 954 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1181, 1213 |
| FaqI | GGGAC | 2 cut(s) | 176, 619 |
| FauI | CCCGC | 1 cut(s) | 77 |
| FblI | GTMKAC | 1 cut(s) | 1290 |
| Fnu4HI | GCNGC | 3 cut(s) | 51, 73, 858 |
| FokI | GGATG | 4 cut(s) | 197, 404, 563, 1145 |
| Fsp4HI | GCNGC | 3 cut(s) | 51, 73, 858 |
| FspBI | CTAG | 1 cut(s) | 1100 |
| GluI | GCNGC | 3 cut(s) | 51, 73, 858 |
| GsaI | CCCAGC | 1 cut(s) | 148 |
| HaeIII | GGCC | 6 cut(s) | 5, 621, 744, 1056, 1167, 1535 |
| HapII | CCGG | 3 cut(s) | 162, 389, 794 |
| HgaI | GACGC | 3 cut(s) | 64, 356, 1266 |
| Hin1I | GRCGYC | 2 cut(s) | 56, 189 |
| HindIII | AAGCTT | 1 cut(s) | 1148 |
| HinfI | GANTC | 4 cut(s) | 979, 986, 1160, 1523 |
| HpaII | CCGG | 3 cut(s) | 162, 389, 794 |
| HphI | GGTGA | 5 cut(s) | 25, 151, 981, 1342, 1411 |
| Hpy166II | GTNNAC | 5 cut(s) | 493, 676, 698, 1016, 1291 |
| Hpy188I | TCNGA | 4 cut(s) | 205, 372, 1269, 1282 |
| Hpy188III | TCNNGA | 7 cut(s) | 182, 612, 983, 1001, 1130, 1140, 1157 |
| Hpy8I | GTNNAC | 5 cut(s) | 493, 676, 698, 1016, 1291 |
| Hpy99I | CGWCG | 3 cut(s) | 58, 357, 1358 |
| HpyAV | CCTTC | 6 cut(s) | 264, 367, 998, 1073, 1483, 1573 |
| HpyCH4III | ACNGT | 4 cut(s) | 448, 488, 923, 1354 |
| HpyCH4IV | ACGT | 6 cut(s) | 177, 189, 227, 355, 586, 963 |
| HpyCH4V | TGCA | 7 cut(s) | 404, 732, 755, 857, 1085, 1296, 1328 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 11, 78, 171, 845, 854, 941, 950, 1082 |
| HpySE526I | ACGT | 6 cut(s) | 177, 189, 227, 355, 586, 963 |
| Hsp92I | GRCGYC | 2 cut(s) | 56, 189 |
| Kzo9I | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| LmnI | GCTCC | 2 cut(s) | 707, 1007 |
| Lsp1109I | GCAGC | 2 cut(s) | 37, 869 |
| LweI | GCATC | 1 cut(s) | 1123 |
| MaeI | CTAG | 1 cut(s) | 1100 |
| MaeII | ACGT | 6 cut(s) | 177, 189, 227, 355, 586, 963 |
| MaeIII | GTNAC | 4 cut(s) | 412, 987, 1399, 1543 |
| MalI | GATC | 5 cut(s) | 63, 155, 219, 1242, 1256 |
| MboI | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| MboII | GAAGA | 5 cut(s) | 9, 197, 1145, 1195, 1505 |
| MflI | RGATCY | 1 cut(s) | 1240 |
| MhlI | GDGCHC | 1 cut(s) | 1447 |
| MlsI | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| MluCI | AATT | 6 cut(s) | 129, 450, 498, 546, 663, 765 |
| MluNI | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| MlyI | GAGTC | 1 cut(s) | 995 |
| MmeI | TCCRAC | 1 cut(s) | 51 |
| Mox20I | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| Mph1103I | ATGCAT | 1 cut(s) | 406 |
| MscI | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| MslI | CAYNNNNRTG | 2 cut(s) | 585, 1131 |
| Msp20I | TGGCCA | 3 cut(s) | 5, 621, 1535 |
| MspI | CCGG | 3 cut(s) | 162, 389, 794 |
| MspR9I | CCNGG | 2 cut(s) | 103, 435 |
| Mva1269I | GAATGC | 2 cut(s) | 648, 734 |
| MvaI | CCWGG | 2 cut(s) | 103, 435 |
| MvnI | CGCG | 1 cut(s) | 367 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 11, 78, 171, 845, 854, 941, 950, 1082 |
| NdeII | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| NmeAIII | GCCGAG | 1 cut(s) | 983 |
| NmuCI | GTSAC | 3 cut(s) | 412, 987, 1399 |
| NsiI | ATGCAT | 1 cut(s) | 406 |
| NspI | RCATGY | 5 cut(s) | 408, 420, 484, 1114, 1379 |
| PaeI | GCATGC | 2 cut(s) | 1114, 1379 |
| PaqCI | CACCTGC | 1 cut(s) | 1001 |
| PciI | ACATGT | 2 cut(s) | 416, 480 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 288 |
| PctI | GAATGC | 2 cut(s) | 648, 734 |
| PfeI | GAWTC | 3 cut(s) | 979, 1160, 1523 |
| PflFI | GACNNNGTC | 1 cut(s) | 366 |
| PkrI | GCNGC | 3 cut(s) | 52, 74, 859 |
| PleI | GAGTC | 1 cut(s) | 994 |
| PpsI | GAGTC | 1 cut(s) | 994 |
| Ppu21I | YACGTR | 1 cut(s) | 228 |
| PscI | ACATGT | 2 cut(s) | 416, 480 |
| PshBI | ATTAAT | 2 cut(s) | 666, 852 |
| Psp6I | CCWGG | 2 cut(s) | 101, 433 |
| PspFI | CCCAGC | 1 cut(s) | 144 |
| PspGI | CCWGG | 2 cut(s) | 101, 433 |
| PspPI | GGNCC | 3 cut(s) | 362, 1192, 1483 |
| PsuI | RGATCY | 1 cut(s) | 1240 |
| PsyI | GACNNNGTC | 1 cut(s) | 366 |
| RsaI | GTAC | 4 cut(s) | 476, 485, 656, 697 |
| RsaNI | GTAC | 4 cut(s) | 475, 484, 655, 696 |
| RseI | CAYNNNNRTG | 2 cut(s) | 585, 1131 |
| SatI | GCNGC | 3 cut(s) | 51, 73, 858 |
| Sau3AI | GATC | 5 cut(s) | 61, 153, 217, 1240, 1254 |
| Sau96I | GGNCC | 3 cut(s) | 362, 1192, 1483 |
| SchI | GAGTC | 1 cut(s) | 995 |
| ScrFI | CCNGG | 2 cut(s) | 103, 435 |
| SduI | GDGCHC | 1 cut(s) | 1447 |
| SfaNI | GCATC | 1 cut(s) | 1123 |
| SfcI | CTRYAG | 1 cut(s) | 919 |
| SgrAI | CRCCGGYG | 1 cut(s) | 161 |
| SinI | GGWCC | 3 cut(s) | 362, 1192, 1483 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 585, 1131 |
| SnaBI | TACGTA | 1 cut(s) | 228 |
| SphI | GCATGC | 2 cut(s) | 1114, 1379 |
| Sse9I | AATT | 6 cut(s) | 129, 450, 498, 546, 663, 765 |
| SsiI | CCGC | 4 cut(s) | 70, 73, 135, 365 |
| SspI | AATATT | 1 cut(s) | 720 |
| SspMI | CTAG | 1 cut(s) | 1100 |
| StyD4I | CCNGG | 2 cut(s) | 101, 433 |
| StyI | CCWWGG | 2 cut(s) | 114, 601 |
| TaaI | ACNGT | 4 cut(s) | 448, 488, 923, 1354 |
| TaiI | ACGT | 6 cut(s) | 180, 192, 230, 358, 589, 966 |
| TaqI | TCGA | 3 cut(s) | 183, 291, 352 |
| TasI | AATT | 6 cut(s) | 129, 450, 498, 546, 663, 765 |
| TatI | WGTACW | 2 cut(s) | 474, 654 |
| TauI | GCSGC | 1 cut(s) | 75 |
| TfiI | GAWTC | 3 cut(s) | 979, 1160, 1523 |
| TscAI | CASTG | 3 cut(s) | 762, 829, 928 |
| TseFI | GTSAC | 3 cut(s) | 412, 987, 1399 |
| TseI | GCWGC | 2 cut(s) | 50, 857 |
| Tsp45I | GTSAC | 3 cut(s) | 412, 987, 1399 |
| TspDTI | ATGAA | 4 cut(s) | 84, 389, 1136, 1310 |
| TspGWI | ACGGA | 1 cut(s) | 1247 |
| TspRI | CASTG | 3 cut(s) | 762, 829, 928 |
| Tth111I | GACNNNGTC | 1 cut(s) | 366 |
| VpaK11BI | GGWCC | 3 cut(s) | 362, 1192, 1483 |
| VspI | ATTAAT | 2 cut(s) | 666, 852 |
| XapI | RAATTY | 3 cut(s) | 129, 498, 765 |
| XceI | RCATGY | 5 cut(s) | 408, 420, 484, 1114, 1379 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 500, 587, 961 |
| XmiI | GTMKAC | 1 cut(s) | 1290 |
| XspI | CTAG | 1 cut(s) | 1100 |
| ZraI | GACGTC | 1 cut(s) | 190 |
| Zsp2I | ATGCAT | 1 cut(s) | 406 |