FvH4_1g23370

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000479
Physical Location & Seq
Forward (+)
15215698 .. 15218489
2792 bp
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UTR
Exon/CDS
Intron
FvH4_1g23370.t1

Sequence Viewer

Length: 2355 bp
ATGGCGTTTGTGGTTTGTTATCTTCTTGCCTTTGCGTTCTTCAATTATGCTGGAGCACAACAACTGCAGTCTAATATAAGCCGGGGCTCTGCTTTAACACCCACTACCAACTCCTCATGGTTGTCTCGTTCTGGTATGTATGCCTTTGGCTTTTACAAGCAAGGCAATGGCTTTGCTGTGGGGATATTTATTGCCGGAATCCCCGAAAAGACTGTAGTGTGGACCGCAAATCGAGACGGTCGACTGGTCTCGGACAATGCCAGCTTGCTCTTCACAAGTGATGGGATTGCCTTGCAGTCGACACAAGGGCAAAGTTTGGTGGTTGATGCTCCGGTGCTTGTTTCCTCTGCTTCGATGCTGGATTCGGGTAATTTTGTGCTGTACAATGCGAGTCGGGCTATAGTATGGCAAAGCTTTGAGTACCCAACTGATACCTTGTTGCCCAATCAACCACTGATAGCAGGGATAGCAGGGAAGGAACTTGTGTCTGCTAAATCAGAAACTGATCACTCATCAGGCACTTTTCGTCTCGCTATGCAAGGTGATGGAAACCTTGTCCAGTACCCTGTGGATACTGGACCAGATGGTGGTACTCAATATGCTTATTATGCGTCTAATACGTTTACAGCAGGACCAAATGTGTCGCTAAATCTAGGTGTTGATGGCCGTCTCTTCTTGCTCAATGGTACTGGTGATGGAAATTTATTGAATCTCAGCCTCGGAGGTCTTCCAACTCAAGGAAGGTCTTATCTTATGAGAATTGATGCGGATGGAATTTTTCGTGTCTATTCGTATGATCTGAAACAGAAAGGAAATTGGTCCACTGAATGGTCATCTTCACAGGATAGATGTGACCCTCAAGGTGCATGTGGATATAATAGCTACTGTGTCACAATGGGAGCAGCAATTGACTGTATATGTCTTCCTGGATTCGAATATGTCAACGCGGGAGATCAGACTTTAGGTTGTGAGAGAAATTCTTCTGTTGGAGATGTTTGCAAATCAAAGAATTGGAATTGCAACTACACCATGCAAGAACTGGGTAGCACAAAATGGGAGGATGAGCCGTATATGGTTCTGAAACCTTCAGGCAAAGAAGATTGCAAACAGGCCTGTTTGGGTGATTTTAACTGTCAGGCTGCAGTTTTCGATGGTTTAAGCTGCAGCAAGCAGAGGCCTCCTTTGAGATATGGAAGAAGAGATGAGAGTACATCAAAGATACTTCTCCTCAAGGAGGTTGTCATGTCTTCTGCACCTCCAGCTCCACGTGCAGTTGTTCCGAAAAGAAGCAGGAAAAATGGTAGAAATTTATTCTTGATTGTTGGCGTGTCATTTACTGCTTTGGGGTTCATTCTGTTGGTGATTTCTGTAACTGTTTTATGGAAAAACAATGTCTGGGCATATAAAAGAATGAGTGAGGTCAATGGTGATGCTGAGTGGAATGAGGATGTGACTCCACGGCCATATGCTTATGAAGATATAGAAAAGATGACTAATAATTTCAATGAAGAGGTTGGTAGAGGAGCCTCAGGAACAGTATACAAAGGGGTGATTGGAAATAGCCAAAAACTAGTTGCTGTCAAGAGACTTGAGAAAGTTGCAGCTGAAGGTGACAGAGAATTTCAGACTGAAATGAGAGTTATTGGCAGAACTCATCACCGGAACTTAGTCCGTTTACTTGGGTATTGTCTTGACGGACCTAAGAAGCTTCTGGTGTATGAGTACATGAGCAATGGTTCACTTGCAGATATACTCTTCACACCTGAGAGGAAACTTCATTGGGAAGAAAGAATGGGAATCGCTCGCAAAATAGCACGAGGTTTTCTTTACCTCCATGAAGAGTGTGATACACAGATCATTCACTGTGACATAAAGCCTCAAAACATACTGATGGATGAGTACATGTGCCCAAAAATAACTGACTTTGGATTATCCAAGCTGCTTCAGCCTGACGAAACCCAAATATGTACTGGTATTCGAGGAACTAAAGGGTATGTGGCACCTGAGTGGCATAGGAAAATCCCTGTAACAGTTAAAGTAGATGTTTACAGCTTTGGAATTGTGCTGTTGGAGATAGTATTCTGTCGAAGGAATGTGGACTGGAGCCTTCCTCATGATGAAGCTATCTTGGATGAATTGGTCTACCATTATTTTGAGAGTGGTGAGCTCAGAAAATTGGTTGGGGATGAAGACATGGACAGCAGGCAATTGGAAAGGGTGGTTAAGGTCGGAATTTGGTGCATCCAGGATGAGCCGTCGCTCCGTCCTTCTATGAAGAAGGTTCTACTTATGTTGGAAGGGACTGTTGAAATCCCAGTTCCTCCTAGTCCGGTTTCATTTCTCAGTACCATCTAG

Protein Analysis

785

Amino Acids

87.01

Weight (kDa)

5.8

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 152 3.6e-14 D-mannose binding lectin
S_locus_glycop PF00954 252 - 312 4.2e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 501 - 762 6.7e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 502 - 762 3.8e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000479)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23370 FvH4_1g23380
malus_domestica MD15G1391000.v1.1 MD15G1391100.v1.1
prunus_persica Prupe.6G158300_v2.0.a1 Prupe.6G158400_v2.0.a1 Prupe.6G158700_v2.0.a1 Prupe.6G158900_v2.0.a1 Prupe.6G159000_v2.0.a1 Prupe.6G164400_v2.0.a1 Prupe.6G164900_v2.0.a1
pyrus_communis pycom15g35040 pycom15g35130 pycom15g35150
rosa_chinensis RchiOBHm_Chr2g0118321 RchiOBHm_Chr2g0118331 RchiOBHm_Chr2g0118351 RchiOBHm_Chr2g0118361 RchiOBHm_Chr2g0118371 RchiOBHm_Chr2g0118591 RchiOBHm_Chr2g0118601 RchiOBHm_Chr2g0118631 RchiOBHm_Chr2g0118641 RchiOBHm_Chr2g0118651
rosa_laevigata RLG00000018409 RLG00000018410 RLG00000018411 RLG00000018412 RLG00000018413 RLG00000018414
rosa_multiflora Rmu_co8003212.1_g000001 Rmu_co8379303.1_g000001 Rmu_co8418889.1_g000001 Rmu_sc0000373.1_g000002 Rmu_sc0000373.1_g000006 Rmu_sc0000373.1_g000013 Rmu_sc0000373.1_g000014 Rmu_sc0000373.1_g000015 Rmu_sc0000373.1_g000017 Rmu_sc0000373.1_g000018 Rmu_sc0008015.1_g000001 Rmu_sc0016207.1_g000001 Rmu_sc0017451.1_g000001
rosa_roxburghii Rroxscaffold_2G00124920 Rroxscaffold_2G00124930 Rroxscaffold_2G00124940 Rroxscaffold_2G00124960 Rroxscaffold_2G00124970 Rroxscaffold_2G00124990 Rroxscaffold_2G00125010 Rroxscaffold_2G00125600 Rroxscaffold_2G00125610 Rroxscaffold_2G00125640 Rroxscaffold_2G00125650 Rroxscaffold_2G00125670 Rroxscaffold_2G00125680
rosa_rugosa Rorug02G0217500 Rorug02G0217600 Rorug02G0217700 Rorug02G0217700 Rorug02G0217700 Rorug02G0217700 Rorug02G0217700 Rorug02G0217800
rosa_samantha Rh2AG273800 Rh2AG273900 Rh2AG274200 Rh2AG274300 Rh2BG285900 Rh2BG286000 Rh2BG286200 Rh2BG286300 Rh2BG286400 Rh2DG281700 Rh2DG281800 Rh2DG281900 Rh2DG282000 Rh2DG299900 Rh2DG300000 Rh2DG300100 Rh2DG300300 Rh2DG300600 Rh2DG300800 Rh2DG301200 Rh2DG301300
rosa_wichuraiana Rw2G021790 Rw2G021800 Rw2G021810 Rw2G021820 Rw2G021830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1999
AccB7I CCANNNNNTGG 2 cut(s) 587, 828
AccI GTMKAC 4 cut(s) 241, 299, 1539, 2142
AccII CGCG 1 cut(s) 947
AciI CCGC 3 cut(s) 225, 767, 947
AcoI YGGCCR 2 cut(s) 664, 1460
AcsI RAATTY 6 cut(s) 700, 774, 976, 1306, 1619, 2232
AcuI CTGAAG 3 cut(s) 1071, 1626, 1928
AcvI CACGTG 1 cut(s) 1268
AfiI CCNNNNNNNGG 4 cut(s) 587, 737, 828, 1234
AflIII ACRYGT 1 cut(s) 1902
AgsI TTSAA 4 cut(s) 43, 709, 1504, 2309
AhlI ACTAGT 1 cut(s) 1570
AjnI CCWGG 2 cut(s) 925, 2244
AleI CACNNNNGTG 1 cut(s) 2005
Alw21I GWGCWC 2 cut(s) 58, 2169
Alw26I GTCTC 6 cut(s) 129, 228, 253, 533, 674, 1579
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 4 cut(s) 664, 1110, 1175, 1460
ApeKI GCWGC 6 cut(s) 902, 1139, 1161, 1164, 1601, 1939
ApoI RAATTY 6 cut(s) 700, 774, 976, 1306, 1619, 2232
Asp700I GAANNNNTTC 1 cut(s) 979
AspS9I GGNCC 5 cut(s) 222, 578, 632, 819, 1697
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 9 cut(s) 554, 704, 1133, 1372, 1439, 1561, 1622, 1649, 2174
AsuII TTCGAA 1 cut(s) 933
AvaII GGWCC 5 cut(s) 222, 578, 632, 819, 1697
AxyI CCTNAGG 1 cut(s) 1528
BaeGI GKGCMC 1 cut(s) 1910
BanI GGYRCC 1 cut(s) 1999
BanII GRGCYC 2 cut(s) 89, 2169
BauI CACGAG 1 cut(s) 1815
BbrPI CACGTG 1 cut(s) 1268
BbsI GAAGAC 4 cut(s) 719, 914, 1239, 2196
Bbv12I GWGCWC 2 cut(s) 58, 2169
BbvI GCAGC 6 cut(s) 914, 1126, 1148, 1176, 1613, 1926
BccI CCATC 8 cut(s) 275, 539, 578, 656, 689, 764, 1145, 1885
BceAI ACGGC 4 cut(s) 651, 1051, 1475, 2239
BciT130I CCWGG 2 cut(s) 927, 2246
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 6 cut(s) 129, 228, 253, 533, 674, 1579
BcuI ACTAGT 1 cut(s) 1570
BfaI CTAG 4 cut(s) 653, 1571, 2325, 2353
BfmI CTRYAG 5 cut(s) 65, 213, 399, 1140, 1162
BfuI GTATCC 1 cut(s) 565
BisI GCNGC 6 cut(s) 903, 1140, 1162, 1165, 1602, 1940
BlsI GCNGC 6 cut(s) 904, 1141, 1163, 1166, 1603, 1941
Bme1390I CCNGG 3 cut(s) 83, 927, 2246
Bme18I GGWCC 5 cut(s) 222, 578, 632, 819, 1697
BmgT120I GGNCC 5 cut(s) 222, 578, 632, 819, 1697
BmiI GGNNCC 3 cut(s) 1525, 2001, 2105
BmrFI CCNGG 3 cut(s) 83, 927, 2246
BmrI ACTGGG 2 cut(s) 1049, 2309
BmsI GCATC 5 cut(s) 316, 345, 754, 1420, 2250
BmuI ACTGGG 2 cut(s) 1049, 2309
BpiI GAAGAC 4 cut(s) 719, 914, 1239, 2196
BplI GAGNNNNNCTC 2 cut(s) 2095, 2127
BpmI CTGGAG 3 cut(s) 72, 1242, 2122
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 4 cut(s) 720, 843, 1214, 1610
BpuMI CCSGG 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 1268
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 82, 718, 1457
BsaWI WCCGGW 3 cut(s) 331, 1659, 2329
Bsc4I CCNNNNNNNGG 4 cut(s) 587, 737, 828, 1234
Bse1I ACTGG 8 cut(s) 249, 559, 580, 694, 1044, 1975, 2105, 2315
Bse21I CCTNAGG 1 cut(s) 1528
Bse3DI GCAATG 2 cut(s) 172, 1738
BseBI CCWGG 2 cut(s) 927, 2246
BseDI CCNNGG 3 cut(s) 82, 718, 1457
BseGI GGATG 8 cut(s) 775, 1066, 1453, 1900, 2137, 2191, 2241, 2254
BseLI CCNNNNNNNGG 4 cut(s) 587, 737, 828, 1234
BseMI GCAATG 2 cut(s) 172, 1738
BseMII CTCAG 6 cut(s) 727, 1425, 1542, 1755, 1995, 2182
BseNI ACTGG 8 cut(s) 249, 559, 580, 694, 1044, 1975, 2105, 2315
BseRI GAGGAG 3 cut(s) 103, 1217, 1536
BseSI GKGCMC 1 cut(s) 1910
BseXI GCAGC 6 cut(s) 914, 1126, 1148, 1176, 1613, 1926
BsgI GTGCAG 2 cut(s) 1236, 1290
Bsh1236I CGCG 1 cut(s) 947
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 4 cut(s) 666, 1112, 1177, 1462
BshNI GGYRCC 1 cut(s) 1999
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 2 cut(s) 58, 2169
BsiSI CCGG 5 cut(s) 82, 195, 332, 1660, 2330
BslFI GGGAC 1 cut(s) 2314
BslI CCNNNNNNNGG 4 cut(s) 587, 737, 828, 1234
BsmAI GTCTC 6 cut(s) 129, 228, 253, 533, 674, 1579
BsmBI CGTCTC 3 cut(s) 228, 533, 674
BsmFI GGGAC 1 cut(s) 2314
BsnI GGCC 4 cut(s) 666, 1112, 1177, 1462
Bso31I GGTCTC 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 4 cut(s) 58, 89, 1910, 2169
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 4 cut(s) 505, 796, 952, 1854
BspACI CCGC 3 cut(s) 225, 767, 947
BspANI GGCC 4 cut(s) 666, 1112, 1177, 1462
BspCNI CTCAG 7 cut(s) 726, 1426, 1541, 1756, 1996, 2181, 2355
BspFNI CGCG 1 cut(s) 947
BspHI TCATGA 1 cut(s) 2113
BspLI GGNNCC 3 cut(s) 1525, 2001, 2105
BspMAI CTGCAG 3 cut(s) 69, 1144, 1166
BspQI GCTCTTC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 933
BspT107I GGYRCC 1 cut(s) 1999
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 172, 1738
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 8 cut(s) 249, 559, 580, 694, 1044, 1975, 2105, 2315
BssECI CCNNGG 3 cut(s) 82, 718, 1457
BssMI GATC 4 cut(s) 505, 796, 952, 1854
BssNAI GTATAC 1 cut(s) 1540
BssSI CACGAG 1 cut(s) 1815
Bst1107I GTATAC 1 cut(s) 1540
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 2 cut(s) 927, 2246
Bst6I CTCTTC 6 cut(s) 275, 677, 1192, 1503, 1760, 1833
BstAUI TGTACA 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 1268
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 5 cut(s) 262, 266, 1169, 1804, 2204
BstDEI CTNAG 9 cut(s) 713, 1434, 1528, 1666, 1701, 1764, 2004, 2168, 2342
BstDSI CCRYGG 1 cut(s) 1457
BstENI CCTNNNNNAGG 1 cut(s) 1232
BstF5I GGATG 8 cut(s) 775, 1066, 1453, 1900, 2137, 2191, 2241, 2254
BstFNI CGCG 1 cut(s) 947
BstKTI GATC 4 cut(s) 508, 799, 955, 1857
BstMAI GTCTC 6 cut(s) 129, 228, 253, 533, 674, 1579
BstMBI GATC 4 cut(s) 505, 796, 952, 1854
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 6 cut(s) 395, 467, 608, 1259, 1268, 1945
BstNI CCWGG 2 cut(s) 927, 2246
BstNSI RCATGY 2 cut(s) 870, 1906
BstSCI CCNGG 3 cut(s) 81, 925, 2244
BstSFI CTRYAG 5 cut(s) 65, 213, 399, 1140, 1162
BstSLI GKGCMC 1 cut(s) 1910
BstUI CGCG 1 cut(s) 947
BstV1I GCAGC 6 cut(s) 914, 1126, 1148, 1176, 1613, 1926
BstV2I GAAGAC 4 cut(s) 719, 914, 1239, 2196
BstZ17I GTATAC 1 cut(s) 1540
Bsu36I CCTNAGG 1 cut(s) 1528
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 4 cut(s) 666, 1112, 1177, 1462
BtgI CCRYGG 1 cut(s) 1457
BtsCI GGATG 8 cut(s) 775, 1066, 1453, 1900, 2137, 2191, 2241, 2254
BtsIMutI CAGTG 3 cut(s) 452, 822, 1861
Cac8I GCNNGC 5 cut(s) 262, 266, 1169, 1804, 2204
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 2113
Cfr13I GGNCC 5 cut(s) 222, 578, 632, 819, 1697
CseI GACGC 1 cut(s) 600
CviAII CATG 9 cut(s) 117, 867, 1030, 1243, 1726, 1835, 1903, 2114, 2194
DdeI CTNAG 9 cut(s) 713, 1434, 1528, 1666, 1701, 1764, 2004, 2168, 2342
DpnI GATC 4 cut(s) 507, 798, 954, 1856
DpnII GATC 4 cut(s) 505, 796, 952, 1854
EaeI YGGCCR 2 cut(s) 664, 1460
Eam1104I CTCTTC 6 cut(s) 275, 677, 1192, 1503, 1760, 1833
EarI CTCTTC 6 cut(s) 275, 677, 1192, 1503, 1760, 1833
Ecl136II GAGCTC 1 cut(s) 2167
Eco147I AGGCCT 2 cut(s) 1112, 1177
Eco24I GRGCYC 2 cut(s) 89, 2169
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 5 cut(s) 222, 578, 632, 819, 1697
Eco53kI GAGCTC 1 cut(s) 2167
Eco57I CTGAAG 3 cut(s) 1071, 1626, 1928
Eco72I CACGTG 1 cut(s) 1268
Eco81I CCTNAGG 1 cut(s) 1528
EcoICRI GAGCTC 1 cut(s) 2167
EcoNI CCTNNNNNAGG 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 925, 2244
EcoT38I GRGCYC 2 cut(s) 89, 2169
Esp3I CGTCTC 3 cut(s) 228, 533, 674
FaeI CATG 9 cut(s) 120, 870, 1033, 1246, 1729, 1838, 1906, 2117, 2197
FaqI GGGAC 1 cut(s) 2314
FatI CATG 9 cut(s) 116, 866, 1029, 1242, 1725, 1834, 1902, 2113, 2193
FauI CCCGC 1 cut(s) 940
FauNDI CATATG 1 cut(s) 1465
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 4 cut(s) 241, 299, 1539, 2142
Fnu4HI GCNGC 6 cut(s) 903, 1140, 1162, 1165, 1602, 1940
FokI GGATG 8 cut(s) 782, 1073, 1460, 1907, 2144, 2198, 2228, 2261
FriOI GRGCYC 2 cut(s) 89, 2169
Fsp4HI GCNGC 6 cut(s) 903, 1140, 1162, 1165, 1602, 1940
FspBI CTAG 4 cut(s) 653, 1571, 2325, 2353
GluI GCNGC 6 cut(s) 903, 1140, 1162, 1165, 1602, 1940
GsuI CTGGAG 3 cut(s) 72, 1242, 2122
HaeIII GGCC 4 cut(s) 666, 1112, 1177, 1462
HapII CCGG 5 cut(s) 82, 195, 332, 1660, 2330
HgaI GACGC 1 cut(s) 600
Hin1II CATG 9 cut(s) 120, 870, 1033, 1246, 1729, 1838, 1906, 2117, 2197
HincII GTYRAC 3 cut(s) 242, 300, 943
HindII GTYRAC 3 cut(s) 242, 300, 943
HindIII AAGCTT 2 cut(s) 412, 1706
HinfI GANTC 7 cut(s) 198, 362, 391, 709, 930, 1453, 1797
HpaII CCGG 5 cut(s) 82, 195, 332, 1660, 2330
HphI GGTGA 9 cut(s) 554, 704, 1133, 1372, 1439, 1561, 1622, 1649, 2174
Hpy188III TCNNGA 6 cut(s) 233, 1315, 1530, 1582, 1691, 2114
Hpy99I CGWCG 1 cut(s) 2260
HpyAV CCTTC 9 cut(s) 469, 735, 1095, 1601, 2082, 2117, 2272, 2277, 2291
HpyCH4IV ACGT 2 cut(s) 620, 1267
HpyF10VI GCNNNNNNNGC 6 cut(s) 395, 467, 608, 1259, 1268, 1945
HpyF3I CTNAG 9 cut(s) 713, 1434, 1528, 1666, 1701, 1764, 2004, 2168, 2342
HpySE526I ACGT 2 cut(s) 620, 1267
Hsp92II CATG 9 cut(s) 120, 870, 1033, 1246, 1729, 1838, 1906, 2117, 2197
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 4 cut(s) 505, 796, 952, 1854
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 7 cut(s) 53, 334, 899, 1267, 1523, 2103, 2265
Lsp1109I GCAGC 6 cut(s) 914, 1126, 1148, 1176, 1613, 1926
LweI GCATC 5 cut(s) 316, 345, 754, 1420, 2250
MaeI CTAG 4 cut(s) 653, 1571, 2325, 2353
MaeII ACGT 2 cut(s) 620, 1267
MaeIII GTNAC 7 cut(s) 851, 889, 1369, 1450, 1610, 1865, 2026
MalI GATC 4 cut(s) 507, 798, 954, 1856
MboI GATC 4 cut(s) 505, 796, 952, 1854
MfeI CAATTG 2 cut(s) 906, 2207
MhlI GDGCHC 4 cut(s) 58, 89, 1910, 2169
MlyI GAGTC 2 cut(s) 400, 1447
MmeI TCCRAC 5 cut(s) 755, 967, 2049, 2209, 2274
MroXI GAANNNNTTC 1 cut(s) 979
MseI TTAA 5 cut(s) 95, 1128, 1157, 2034, 2223
MslI CAYNNNNRTG 2 cut(s) 1889, 2005
MspA1I CMGCKG 1 cut(s) 1604
MspI CCGG 5 cut(s) 82, 195, 332, 1660, 2330
MspR9I CCNGG 3 cut(s) 83, 927, 2246
MunI CAATTG 2 cut(s) 906, 2207
MvaI CCWGG 2 cut(s) 927, 2246
MvnI CGCG 1 cut(s) 947
MwoI GCNNNNNNNGC 6 cut(s) 395, 467, 608, 1259, 1268, 1945
NciI CCSGG 1 cut(s) 83
NdeI CATATG 1 cut(s) 1465
NdeII GATC 4 cut(s) 505, 796, 952, 1854
NlaIII CATG 9 cut(s) 120, 870, 1033, 1246, 1729, 1838, 1906, 2117, 2197
NlaIV GGNNCC 3 cut(s) 1525, 2001, 2105
NmuCI GTSAC 5 cut(s) 851, 889, 1450, 1610, 1865
NspI RCATGY 2 cut(s) 870, 1906
NspV TTCGAA 1 cut(s) 933
OliI CACNNNNGTG 1 cut(s) 2005
PagI TCATGA 1 cut(s) 2113
PceI AGGCCT 2 cut(s) 1112, 1177
PciI ACATGT 1 cut(s) 1902
PciSI GCTCTTC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 979
PfeI GAWTC 5 cut(s) 198, 362, 709, 930, 1797
PflMI CCANNNNNTGG 2 cut(s) 587, 828
PfoI TCCNGGA 2 cut(s) 925, 2244
PkrI GCNGC 6 cut(s) 904, 1141, 1163, 1166, 1603, 1941
PleI GAGTC 2 cut(s) 399, 1447
PmaCI CACGTG 1 cut(s) 1268
PmlI CACGTG 1 cut(s) 1268
PpsI GAGTC 2 cut(s) 399, 1447
Ppu21I YACGTR 1 cut(s) 1268
PscI ACATGT 1 cut(s) 1902
Psp124BI GAGCTC 1 cut(s) 2169
Psp6I CCWGG 2 cut(s) 925, 2244
PspCI CACGTG 1 cut(s) 1268
PspGI CCWGG 2 cut(s) 925, 2244
PspN4I GGNNCC 3 cut(s) 1525, 2001, 2105
PspPI GGNCC 5 cut(s) 222, 578, 632, 819, 1697
PstI CTGCAG 3 cut(s) 69, 1144, 1166
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 1604
RseI CAYNNNNRTG 2 cut(s) 1889, 2005
SacI GAGCTC 1 cut(s) 2169
SalI GTCGAC 2 cut(s) 240, 298
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 5 cut(s) 95, 1128, 1157, 2034, 2223
SatI GCNGC 6 cut(s) 903, 1140, 1162, 1165, 1602, 1940
Sau3AI GATC 4 cut(s) 505, 796, 952, 1854
Sau96I GGNCC 5 cut(s) 222, 578, 632, 819, 1697
SchI GAGTC 2 cut(s) 400, 1447
ScrFI CCNGG 3 cut(s) 83, 927, 2246
SduI GDGCHC 4 cut(s) 58, 89, 1910, 2169
SfaNI GCATC 5 cut(s) 316, 345, 754, 1420, 2250
SfcI CTRYAG 5 cut(s) 65, 213, 399, 1140, 1162
SfuI TTCGAA 1 cut(s) 933
SinI GGWCC 5 cut(s) 222, 578, 632, 819, 1697
SmiMI CAYNNNNRTG 2 cut(s) 1889, 2005
SmlI CTYRAG 4 cut(s) 735, 858, 1229, 1589
SmoI CTYRAG 4 cut(s) 735, 858, 1229, 1589
SpeI ACTAGT 1 cut(s) 1570
SseBI AGGCCT 2 cut(s) 1112, 1177
SsiI CCGC 3 cut(s) 225, 767, 947
SspMI CTAG 4 cut(s) 653, 1571, 2325, 2353
SstI GAGCTC 1 cut(s) 2169
StuI AGGCCT 2 cut(s) 1112, 1177
StyD4I CCNGG 3 cut(s) 81, 925, 2244
TaiI ACGT 2 cut(s) 623, 1270
TaqI TCGA 8 cut(s) 232, 241, 299, 353, 933, 1149, 1978, 2086
TatI WGTACW 5 cut(s) 381, 1208, 1722, 1899, 1967
TfiI GAWTC 5 cut(s) 198, 362, 709, 930, 1797
Tru1I TTAA 5 cut(s) 95, 1128, 1157, 2034, 2223
Tru9I TTAA 5 cut(s) 95, 1128, 1157, 2034, 2223
TscAI CASTG 3 cut(s) 459, 829, 1868
TseFI GTSAC 5 cut(s) 851, 889, 1450, 1610, 1865
TseI GCWGC 6 cut(s) 902, 1139, 1161, 1164, 1601, 1939
Tsp45I GTSAC 5 cut(s) 851, 889, 1450, 1610, 1865
TspGWI ACGGA 3 cut(s) 1661, 1710, 2252
TspRI CASTG 3 cut(s) 459, 829, 1868
Van91I CCANNNNNTGG 2 cut(s) 587, 828
VpaK11BI GGWCC 5 cut(s) 222, 578, 632, 819, 1697
XagI CCTNNNNNAGG 1 cut(s) 1232
XapI RAATTY 6 cut(s) 700, 774, 976, 1306, 1619, 2232
XceI RCATGY 2 cut(s) 870, 1906
XcmI CCANNNNNNNNNTGG 2 cut(s) 1036, 1967
XmiI GTMKAC 4 cut(s) 241, 299, 1539, 2142
XmnI GAANNNNTTC 1 cut(s) 979
XspI CTAG 4 cut(s) 653, 1571, 2325, 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.