FvH4_3g14820

Somatic embryogenesis receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001737
Physical Location & Seq
Reverse (-)
9149505 .. 9150095
591 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14820.t1

Sequence Viewer

Length: 591 bp
ATGGCGACGACATGCTGGATGTTATTTGCTCTAACTCTAAGCGCAAGTCTCATTCCTGGAGCATATCCGCTCTCAGAGACAGAAGCTTTGATCGCGTTTAAGAAGAGCTTGGCTGATCCTCTAAACGTGCTCAAGACCTGGGATGCCAGTCAACCTAACCCATGTAGTTGGGAACACATTACCTGCAATGACCAAAATAAGGTCAATGGAGTGTTCTTAACTAAAAGCCAGCTCACTGGACAACTGGTACCTGAACTTGGATCGCTTTCGGCTCTCCATCAGATGATTCTTGATCAGAATGAAATCTCAGGACCAATTCCAGCTGAGCTTGGTAACCTGAGTATCCTGAGGGGTCTCGTATTAAGTTATAATGGATTAACAGGCAGCATTCCTCCTTCATTGGGTAATTTGAATTATCTTCTTACCTTTCAACTCGATCATAACCAATTATCTGGGAAAATCCCTTCTGAAATTGCATTAATCAAAAGCCTCCAGATCGCGGCTTTTAACGATAACAACGATTTATGTTGGGATTTTCAGCCCACACCAATATTTAAAGGCGATTATCCATTATGCCAGAAGATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_4 LRR_8

Protein Analysis

197

Amino Acids

21.43

Weight (kDa)

4.86

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 63 9.7e-14 Leucine rich repeat N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 191
Acc65I GGTACC 1 cut(s) 247
AccB1I GGYRCC 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 70
AccII CGCG 2 cut(s) 95, 500
AciI CCGC 2 cut(s) 68, 500
AclWI GGATC 2 cut(s) 110, 268
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 199, 257, 401, 499
AgsI TTSAA 2 cut(s) 412, 431
AjnI CCWGG 2 cut(s) 55, 137
AluBI AGCT 5 cut(s) 86, 108, 232, 323, 328
AluI AGCT 5 cut(s) 86, 108, 232, 323, 328
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 3 cut(s) 53, 71, 359
AlwI GGATC 2 cut(s) 110, 268
ApeKI GCWGC 1 cut(s) 384
ArsI GACNNNNNNTTYG 2 cut(s) 70, 102
AseI ATTAAT 1 cut(s) 479
Asp718I GGTACC 1 cut(s) 247
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 311
AvaII GGWCC 1 cut(s) 311
AxyI CCTNAGG 1 cut(s) 347
BaeI ACNNNNGTAYC 2 cut(s) 325, 358
BanI GGYRCC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 1 cut(s) 396
BccI CCATC 1 cut(s) 285
BciT130I CCWGG 2 cut(s) 57, 139
BciVI GTATCC 1 cut(s) 353
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 3 cut(s) 53, 71, 359
BfuAI ACCTGC 1 cut(s) 191
BfuI GTATCC 1 cut(s) 353
BisI GCNGC 2 cut(s) 385, 501
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 2 cut(s) 386, 502
Bme1390I CCNGG 2 cut(s) 57, 139
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 57, 139
BmsI GCATC 2 cut(s) 133, 573
BpmI CTGGAG 2 cut(s) 78, 476
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 1 cut(s) 138
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 257, 401, 499
Bse1I ACTGG 3 cut(s) 147, 241, 249
Bse21I CCTNAGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 2 cut(s) 57, 139
BseDI CCNNGG 1 cut(s) 138
BseGI GGATG 2 cut(s) 24, 148
BseLI CCNNNNNNNGG 4 cut(s) 199, 257, 401, 499
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 5 cut(s) 87, 315, 321, 329, 338
BseNI ACTGG 3 cut(s) 147, 241, 249
BseXI GCAGC 1 cut(s) 396
Bsh1236I CGCG 2 cut(s) 95, 500
BshNI GGYRCC 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 132
BslI CCNNNNNNNGG 4 cut(s) 199, 257, 401, 499
BsmAI GTCTC 3 cut(s) 53, 71, 359
BsmI GAATGC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 6 cut(s) 90, 115, 260, 292, 436, 495
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 2 cut(s) 68, 500
BspCNI CTCAG 5 cut(s) 86, 316, 320, 330, 339
BspFNI CGCG 2 cut(s) 95, 500
BspLI GGNNCC 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 2 cut(s) 110, 268
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 193
BsrI ACTGG 3 cut(s) 147, 241, 249
BssECI CCNNGG 1 cut(s) 138
BssMI GATC 6 cut(s) 90, 115, 260, 292, 436, 495
Bst2UI CCWGG 2 cut(s) 57, 139
Bst6I CTCTTC 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 6 cut(s) 38, 73, 307, 324, 338, 347
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 2 cut(s) 24, 148
BstFNI CGCG 2 cut(s) 95, 500
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 6 cut(s) 93, 118, 263, 295, 439, 498
BstMAI GTCTC 3 cut(s) 53, 71, 359
BstMBI GATC 6 cut(s) 90, 115, 260, 292, 436, 495
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNI CCWGG 2 cut(s) 57, 139
BstNSI RCATGY 1 cut(s) 15
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 55, 137
BstUI CGCG 2 cut(s) 95, 500
BstV1I GCAGC 1 cut(s) 396
BstXI CCANNNNNNTGG 3 cut(s) 168, 236, 452
Bsu36I CCTNAGG 1 cut(s) 347
BsuI GTATCC 1 cut(s) 353
BtsCI GGATG 2 cut(s) 24, 148
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 248
CviAII CATG 2 cut(s) 12, 162
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 6 cut(s) 38, 73, 307, 324, 338, 347
DpnI GATC 6 cut(s) 92, 117, 262, 294, 438, 497
DpnII GATC 6 cut(s) 90, 115, 260, 292, 436, 495
DraI TTTAAA 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 55, 137
FaeI CATG 2 cut(s) 15, 165
FaiI YATR 7 cut(s) 13, 64, 163, 369, 441, 526, 574
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 2 cut(s) 11, 161
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 385, 501
FokI GGATG 2 cut(s) 31, 155
Fsp4HI GCNGC 2 cut(s) 385, 501
GlaI GCGC 1 cut(s) 43
GluI GCNGC 2 cut(s) 385, 501
GsuI CTGGAG 2 cut(s) 78, 476
HhaI GCGC 1 cut(s) 44
Hin1II CATG 2 cut(s) 15, 165
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 84
HinfI GANTC 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 152
Hpy188I TCNGA 4 cut(s) 76, 282, 297, 469
Hpy188III TCNNGA 5 cut(s) 133, 290, 309, 346, 493
Hpy8I GTNNAC 1 cut(s) 152
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 405, 474
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 3 cut(s) 186, 476, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 6 cut(s) 38, 73, 307, 324, 338, 347
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 15, 165
HspAI GCGC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 6 cut(s) 90, 115, 260, 292, 436, 495
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 2 cut(s) 133, 573
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 332
MalI GATC 6 cut(s) 92, 117, 262, 294, 438, 497
MbiI CCGCTC 1 cut(s) 70
MboI GATC 6 cut(s) 90, 115, 260, 292, 436, 495
MboII GAAGA 2 cut(s) 115, 410
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 5 cut(s) 315, 406, 412, 446, 471
MnlI CCTC 4 cut(s) 129, 342, 402, 500
MseI TTAA 7 cut(s) 99, 218, 362, 377, 479, 507, 555
MspA1I CMGCKG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 57, 139
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 2 cut(s) 57, 139
MvnI CGCG 2 cut(s) 95, 500
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 6 cut(s) 90, 115, 260, 292, 436, 495
NlaIII CATG 2 cut(s) 15, 165
NlaIV GGNNCC 1 cut(s) 249
NspI RCATGY 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 55
PkrI GCNGC 2 cut(s) 386, 502
PshBI ATTAAT 1 cut(s) 479
PsiI TTATAA 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 55, 137
PspEI GGTNACC 1 cut(s) 332
PspGI CCWGG 2 cut(s) 55, 137
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 7 cut(s) 99, 218, 362, 377, 479, 507, 555
SatI GCNGC 2 cut(s) 385, 501
Sau3AI GATC 6 cut(s) 90, 115, 260, 292, 436, 495
Sau96I GGNCC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 57, 139
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 2 cut(s) 133, 573
SinI GGWCC 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 5 cut(s) 315, 406, 412, 446, 471
SsiI CCGC 2 cut(s) 68, 500
SspI AATATT 1 cut(s) 552
StyD4I CCNGG 2 cut(s) 55, 137
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 435
TasI AATT 5 cut(s) 315, 406, 412, 446, 471
TauI GCSGC 1 cut(s) 503
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 7 cut(s) 99, 218, 362, 377, 479, 507, 555
Tru9I TTAA 7 cut(s) 99, 218, 362, 377, 479, 507, 555
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 2 cut(s) 315, 387
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 311
VspI ATTAAT 1 cut(s) 479
XceI RCATGY 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.