MD11G1182200.v1.1

Somatic embryogenesis receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001737
Physical Location & Seq
Forward (+)
22450724 .. 22455859
5136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1182200.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGCGGCGGCATCCAGGTTCTTCTTCACTCTAACCCTAACCCTAAGCGTATGCCTCCTTCCGCTGGTGTATGCCAACTCCGAAGGCGACGCTTTGTACACGTTCCGGCGGAGCTTGTCGGATCCCGATAACCTGCTCCAGAGCTGGGATCCTACCCTCGTCAACCCTTGCACTTGGTTTCACATCACCTGCAACCAGGACAATCGCGTTACCCGAGTGGATTTAGGTAATTCAAACCTCTCTGGACATTTGGTACCTGAACTAGGAAAGCTTGAGCATCTTCAATATCTGGAGCTTTACAAAAATAACATTCAAGGAACTATTCCAGCTGAGCTTGGTAACCTGAAGAGCCTCATTAGCTTGGACTTATATAACAACAACATATCGGGGACCATTCCTCCGTCGTTGGGGAAATTGAAATCACTTGTGTTTTTGCGTCTCAATGACAATAGGTTAACTGGGCCAATCCCCAGGGATCTTACGGGTATTTCAAGCCTCAAAGTTGTGGATGTATCAAACAATAATTTATGTGGGACGATTCCTACCAGTGGACCATTTGAGCACATCCCCTTGAACAACTTTGAGAACAACCATCGACTCGAAGGTCCAGAATTGTTGGGGCTTGCAAGTTACGATACAAACTGCTCGTAA

Protein Analysis

217

Amino Acids

23.87

Weight (kDa)

5.47

Isoelectric Point (pI)

30.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 65 3.8e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 74 - 171 3.7e-14 Leucine-rich repeat region
LRR_8 PF13855 93 - 152 1.5e-07 Leucine rich repeat
LRR_4 PF12799 93 - 133 6.5e-06 Leucine Rich repeats (2 copies)
LRR_4 PF12799 116 - 152 4.6e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 197
AasI GACNNNNNNGTC 1 cut(s) 603
Acc36I ACCTGC 2 cut(s) 141, 197
Acc65I GGTACC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 253
AccII CGCG 1 cut(s) 207
AciI CCGC 4 cut(s) 5, 8, 62, 109
AclWI GGATC 5 cut(s) 116, 129, 143, 156, 483
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 2 cut(s) 98, 255
AfiI CCNNNNNNNGG 5 cut(s) 64, 145, 263, 407, 548
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 6 cut(s) 234, 284, 314, 418, 492, 574
AjnI CCWGG 3 cut(s) 14, 195, 470
AluBI AGCT 7 cut(s) 114, 144, 271, 295, 329, 334, 360
AluI AGCT 7 cut(s) 114, 144, 271, 295, 329, 334, 360
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 5 cut(s) 116, 129, 143, 156, 483
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 461
Asp718I GGTACC 1 cut(s) 253
AspS9I GGNCC 4 cut(s) 390, 461, 551, 605
AsuHPI GGTGA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 3 cut(s) 390, 551, 605
BamHI GGATCC 2 cut(s) 121, 148
BanI GGYRCC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
Bbv12I GWGCWC 1 cut(s) 564
BccI CCATC 1 cut(s) 600
BciT130I CCWGG 3 cut(s) 16, 197, 472
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 141, 197
BisI GCNGC 2 cut(s) 6, 9
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 3 cut(s) 16, 197, 472
Bme18I GGWCC 3 cut(s) 390, 551, 605
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 4 cut(s) 390, 461, 551, 605
BmiI GGNNCC 4 cut(s) 123, 150, 255, 391
BmrFI CCNGG 3 cut(s) 16, 197, 472
BmrI ACTGGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 20, 286
BmuI ACTGGG 1 cut(s) 468
BpmI CTGGAG 2 cut(s) 122, 311
Bpu10I CCTNAGC 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 470, 471
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 145, 263, 407, 548
Bse1I ACTGG 2 cut(s) 463, 546
BseBI CCWGG 3 cut(s) 16, 197, 472
BseDI CCNNGG 2 cut(s) 470, 471
BseGI GGATG 3 cut(s) 11, 514, 564
BseLI CCNNNNNNNGG 5 cut(s) 64, 145, 263, 407, 548
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 463, 546
BseYI CCCAGC 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 463
BshNI GGYRCC 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 2 cut(s) 403, 547
BslI CCNNNNNNNGG 5 cut(s) 64, 145, 263, 407, 548
BsmAI GTCTC 1 cut(s) 443
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 2 cut(s) 403, 547
BsnI GGCC 1 cut(s) 463
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 564
Bsp1407I TGTACA 1 cut(s) 96
Bsp143I GATC 3 cut(s) 121, 148, 475
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 4 cut(s) 5, 8, 62, 109
BspANI GGCC 1 cut(s) 463
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 207
BspLI GGNNCC 4 cut(s) 123, 150, 255, 391
BspMI ACCTGC 2 cut(s) 141, 197
BspPI GGATC 5 cut(s) 116, 129, 143, 156, 483
BspQI GCTCTTC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 253
BsrGI TGTACA 1 cut(s) 96
BsrI ACTGG 2 cut(s) 463, 546
BssECI CCNNGG 2 cut(s) 470, 471
BssMI GATC 3 cut(s) 121, 148, 475
Bst2UI CCWGG 3 cut(s) 16, 197, 472
Bst6I CTCTTC 1 cut(s) 341
BstAUI TGTACA 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 2 cut(s) 44, 330
BstEII GGTNACC 1 cut(s) 338
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 3 cut(s) 11, 514, 564
BstFNI CGCG 1 cut(s) 207
BstKTI GATC 3 cut(s) 124, 151, 478
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 3 cut(s) 121, 148, 475
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNI CCWGG 3 cut(s) 16, 197, 472
BstPI GGTNACC 1 cut(s) 338
BstSCI CCNGG 3 cut(s) 14, 195, 470
BstUI CGCG 1 cut(s) 207
BstX2I RGATCY 3 cut(s) 121, 148, 475
BstYI RGATCY 3 cut(s) 121, 148, 475
BsuRI GGCC 1 cut(s) 463
BtsCI GGATG 3 cut(s) 11, 514, 564
BtsIMutI CAGTG 1 cut(s) 553
BveI ACCTGC 2 cut(s) 141, 197
Cac8I GCNNGC 1 cut(s) 624
Cfr13I GGNCC 4 cut(s) 390, 461, 551, 605
CseI GACGC 2 cut(s) 98, 425
Csp6I GTAC 2 cut(s) 97, 254
CviQI GTAC 2 cut(s) 97, 254
DdeI CTNAG 2 cut(s) 44, 330
DpnI GATC 3 cut(s) 123, 150, 477
DpnII GATC 3 cut(s) 121, 148, 475
DrdI GACNNNNNNGTC 1 cut(s) 603
DseDI GACNNNNNNGTC 1 cut(s) 603
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
EciI GGCGGA 1 cut(s) 124
Eco47I GGWCC 3 cut(s) 390, 551, 605
Eco57I CTGAAG 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 213
Eco91I GGTNACC 1 cut(s) 338
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO65I GGTNACC 1 cut(s) 338
EcoRII CCWGG 3 cut(s) 14, 195, 470
Esp3I CGTCTC 1 cut(s) 443
FaiI YATR 6 cut(s) 52, 72, 370, 372, 383, 529
FaqI GGGAC 2 cut(s) 403, 547
Fnu4HI GCNGC 2 cut(s) 6, 9
FokI GGATG 2 cut(s) 521, 551
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 263
GluI GCNGC 2 cut(s) 6, 9
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 2 cut(s) 122, 311
HaeIII GGCC 1 cut(s) 463
HapII CCGG 1 cut(s) 106
HgaI GACGC 2 cut(s) 98, 425
HincII GTYRAC 2 cut(s) 163, 456
HindII GTYRAC 2 cut(s) 163, 456
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 2 cut(s) 538, 597
HpaI GTTAAC 1 cut(s) 456
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 99, 163, 456, 551
Hpy188I TCNGA 2 cut(s) 82, 121
Hpy188III TCNNGA 5 cut(s) 125, 139, 243, 290, 608
Hpy8I GTNNAC 4 cut(s) 99, 163, 456, 551
Hpy99I CGWCG 2 cut(s) 92, 406
HpyAV CCTTC 3 cut(s) 68, 77, 596
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 171, 192, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 2 cut(s) 44, 330
HpySE526I ACGT 1 cut(s) 101
KpnI GGTACC 1 cut(s) 257
KspAI GTTAAC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 121, 148, 475
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 3 cut(s) 111, 141, 292
LweI GCATC 2 cut(s) 20, 286
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 208, 338, 629
MalI GATC 3 cut(s) 123, 150, 477
MboI GATC 3 cut(s) 121, 148, 475
MboII GAAGA 4 cut(s) 13, 16, 272, 358
MflI RGATCY 3 cut(s) 121, 148, 475
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 4 cut(s) 229, 413, 523, 611
MlyI GAGTC 1 cut(s) 591
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 6 cut(s) 65, 167, 248, 362, 408, 506
MseI TTAA 1 cut(s) 455
MspA1I CMGCKG 2 cut(s) 64, 329
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 3 cut(s) 16, 197, 472
MvaI CCWGG 3 cut(s) 16, 197, 472
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 357
NdeII GATC 3 cut(s) 121, 148, 475
NlaIV GGNNCC 4 cut(s) 123, 150, 255, 391
PaqCI CACCTGC 1 cut(s) 197
PasI CCCWGGG 1 cut(s) 471
PciSI GCTCTTC 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PfeI GAWTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 1 cut(s) 591
PpsI GAGTC 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 14, 195, 470
PspEI GGTNACC 1 cut(s) 338
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 3 cut(s) 14, 195, 470
PspN4I GGNNCC 4 cut(s) 123, 150, 255, 391
PspPI GGNCC 4 cut(s) 390, 461, 551, 605
PsuI RGATCY 3 cut(s) 121, 148, 475
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 98, 255
RsaNI GTAC 2 cut(s) 97, 254
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 1 cut(s) 455
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 3 cut(s) 121, 148, 475
Sau96I GGNCC 4 cut(s) 390, 461, 551, 605
SchI GAGTC 1 cut(s) 591
ScrFI CCNGG 3 cut(s) 16, 197, 472
SduI GDGCHC 1 cut(s) 564
SfaNI GCATC 2 cut(s) 20, 286
SinI GGWCC 3 cut(s) 390, 551, 605
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 4 cut(s) 229, 413, 523, 611
SsiI CCGC 4 cut(s) 5, 8, 62, 109
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 14, 195, 470
TaiI ACGT 1 cut(s) 104
TaqI TCGA 2 cut(s) 595, 600
TasI AATT 4 cut(s) 229, 413, 523, 611
TatI WGTACW 1 cut(s) 96
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TscAI CASTG 1 cut(s) 553
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 3 cut(s) 390, 551, 605
XagI CCTNNNNNAGG 1 cut(s) 261
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.